RU2748026C2 - Способы и композиции для секреции гетерологичных полипептидов - Google Patents

Способы и композиции для секреции гетерологичных полипептидов Download PDF

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RU2748026C2
RU2748026C2 RU2016140244A RU2016140244A RU2748026C2 RU 2748026 C2 RU2748026 C2 RU 2748026C2 RU 2016140244 A RU2016140244 A RU 2016140244A RU 2016140244 A RU2016140244 A RU 2016140244A RU 2748026 C2 RU2748026 C2 RU 2748026C2
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antibody
signal peptide
polypeptide
polynucleotide
variant
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RU2016140244A
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RU2016140244A3 (enExample
RU2016140244A (ru
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Доротея РЕЙЛЛИ
Ичжоу ЧЖОУ
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Дженентек, Инк.
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/30—Immunoglobulins specific features characterized by aspects of specificity or valency
    • C07K2317/31—Immunoglobulins specific features characterized by aspects of specificity or valency multispecific
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
RU2016140244A 2014-03-14 2015-03-16 Способы и композиции для секреции гетерологичных полипептидов RU2748026C2 (ru)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US201461953629P 2014-03-14 2014-03-14
US61/953,629 2014-03-14
US201562107981P 2015-01-26 2015-01-26
US62/107,981 2015-01-26
US201562108476P 2015-01-27 2015-01-27
US62/108,476 2015-01-27
PCT/US2015/020783 WO2015139046A1 (en) 2014-03-14 2015-03-16 Methods and compositions for secretion of heterologous polypeptides

Publications (3)

Publication Number Publication Date
RU2016140244A RU2016140244A (ru) 2018-04-17
RU2016140244A3 RU2016140244A3 (enExample) 2019-05-22
RU2748026C2 true RU2748026C2 (ru) 2021-05-19

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RU2016140244A RU2748026C2 (ru) 2014-03-14 2015-03-16 Способы и композиции для секреции гетерологичных полипептидов

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US (2) US10435694B2 (enExample)
EP (1) EP3116999B1 (enExample)
JP (2) JP6644717B2 (enExample)
KR (1) KR102561695B1 (enExample)
CN (1) CN106103730B (enExample)
AU (1) AU2015229035B2 (enExample)
BR (1) BR112016020822A2 (enExample)
CA (1) CA2941687A1 (enExample)
ES (1) ES2897765T3 (enExample)
HR (1) HRP20211748T1 (enExample)
IL (1) IL247632B (enExample)
MA (1) MA39746A (enExample)
MX (2) MX2016011637A (enExample)
PL (1) PL3116999T3 (enExample)
RU (1) RU2748026C2 (enExample)
SG (1) SG11201607519VA (enExample)
SI (1) SI3116999T1 (enExample)
WO (1) WO2015139046A1 (enExample)

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SG11201607519VA (en) * 2014-03-14 2016-10-28 Genentech Inc Methods and compositions for secretion of heterologous polypeptides
EP3532618A4 (en) * 2016-10-27 2020-05-20 MedImmune, LLC SIGNAL POLYPEPTIDES FOR IMPROVED PROTEIN SECRETION
CN106676122B (zh) * 2017-01-24 2019-10-29 成都分子脉象生物科技有限公司 一种基于AraF信号肽的双功能DNA元件及其应用
US20210163607A1 (en) 2017-05-19 2021-06-03 Nippon Zenyaku Kogyo Co., Ltd. Method for suppressing regulatory t cell infiltration by ccr4 inhibition and method for treating canine neoplastic disease
GB201708277D0 (en) * 2017-05-24 2017-07-05 Ge Healthcare A Recombinant protein
KR101946789B1 (ko) * 2018-01-26 2019-02-12 (주)휴온스 이황화결합 이성질화효소 신호 펩타이드를 포함하는 재조합 벡터 및 이의 용도
JP2022514863A (ja) * 2018-12-19 2022-02-16 ヴェルサメブ アーゲー タンパク質をコードするｒｎａ
GB2590642B (en) * 2019-12-20 2024-02-14 Kymab Ltd Improved lambda antibodies
ES3026707T3 (en) * 2019-08-05 2025-06-12 Wacker Chemie Ag Bacterial strain for releasing a recombinant protein in a fermentation method
SE544059C2 (en) * 2020-02-07 2021-11-30 Cloneopt Ab Synthetically evolved dna constructs for regulating signal peptide performance as well as vectors and host cells thereof
SE543945C2 (en) * 2020-02-07 2021-10-05 Cloneopt Ab Synthetically evolved DNA constructs for regulating signal peptide performance as well as vectors and host cells thereof
US12570987B2 (en) 2020-02-07 2026-03-10 Cloneopt Ab Synthetically evolved DNA constructs for regulating signal peptide performance as well as vectors, host cells and recombinant proteins thereof
US20250368732A1 (en) * 2021-09-24 2025-12-04 Xbrane Biopharma Ab Dna constructs and host cells for expressing recombinant protein
WO2026132369A1 (en) * 2024-12-22 2026-06-25 F. Hoffmann-La Roche Ag Method for the generation of a bivalent, bispecific fragment antigen binding expressing mammalian cells

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