RU2299214C2 - СПОСОБ ВЫДЕЛЕНИЯ И ОЧИСТКИ РЕКОМБИНАНТНЫХ ВАРИАНТОВ Bet v 1 - Google Patents

СПОСОБ ВЫДЕЛЕНИЯ И ОЧИСТКИ РЕКОМБИНАНТНЫХ ВАРИАНТОВ Bet v 1 Download PDF

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Publication number
RU2299214C2
RU2299214C2 RU2003109430/13A RU2003109430A RU2299214C2 RU 2299214 C2 RU2299214 C2 RU 2299214C2 RU 2003109430/13 A RU2003109430/13 A RU 2003109430/13A RU 2003109430 A RU2003109430 A RU 2003109430A RU 2299214 C2 RU2299214 C2 RU 2299214C2
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RU
Russia
Prior art keywords
purification
nacl
chromatographic
solution
recombinant
Prior art date
Application number
RU2003109430/13A
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English (en)
Russian (ru)
Other versions
RU2003109430A (ru
Inventor
Роланд ЗУКК (DE)
Роланд ЗУКК
Оливер КРОМВЕЛЛЬ (DE)
Оливер КРОМВЕЛЛЬ
Хельмут ФИБИГ (DE)
Хельмут ФИБИГ
Original Assignee
Мерк Патент Гмбх
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Мерк Патент Гмбх filed Critical Мерк Патент Гмбх
Publication of RU2003109430A publication Critical patent/RU2003109430A/ru
Application granted granted Critical
Publication of RU2299214C2 publication Critical patent/RU2299214C2/ru

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
    • C07K1/1136—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by reversible modification of the secondary, tertiary or quarternary structure, e.g. using denaturating or stabilising agents
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Botany (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
RU2003109430/13A 2000-09-08 2001-08-18 СПОСОБ ВЫДЕЛЕНИЯ И ОЧИСТКИ РЕКОМБИНАНТНЫХ ВАРИАНТОВ Bet v 1 RU2299214C2 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10044360.5 2000-09-08
DE10044360A DE10044360A1 (de) 2000-09-08 2000-09-08 Verfahren zur Aufreinigung von rekombinanten als unlösliche Aggregate exprimierte Proteinen

Publications (2)

Publication Number Publication Date
RU2003109430A RU2003109430A (ru) 2004-10-27
RU2299214C2 true RU2299214C2 (ru) 2007-05-20

Family

ID=7655449

Family Applications (1)

Application Number Title Priority Date Filing Date
RU2003109430/13A RU2299214C2 (ru) 2000-09-08 2001-08-18 СПОСОБ ВЫДЕЛЕНИЯ И ОЧИСТКИ РЕКОМБИНАНТНЫХ ВАРИАНТОВ Bet v 1

Country Status (14)

Country Link
US (1) US6900034B2 (https=)
EP (1) EP1315740B1 (https=)
JP (1) JP5174312B2 (https=)
CN (1) CN1468255A (https=)
AT (1) ATE443712T1 (https=)
AU (2) AU8769501A (https=)
CA (1) CA2421438C (https=)
DE (2) DE10044360A1 (https=)
ES (1) ES2333939T3 (https=)
HU (1) HUP0303554A2 (https=)
PL (1) PL206158B1 (https=)
PT (1) PT1315740E (https=)
RU (1) RU2299214C2 (https=)
WO (1) WO2002020559A1 (https=)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1478662B2 (de) * 2002-02-27 2017-07-05 Merck Patent GmbH Verfahren zur herstellung von hypoallergenem birkenpollenhauptallergen rbet v 1
CA2487673C (en) * 2003-12-02 2010-11-02 F. Hoffmann-La Roche Ag Improved method for the recombinant production and purification of protein kinases
WO2010015697A1 (en) * 2008-08-08 2010-02-11 Janssen Pharmaceutica Nv Anti-allergen combinations of calcium and lanthanum salts
EP2721051B1 (en) 2011-06-15 2017-11-29 ASIT BioTech s.a. A method for the production of hydrolyzed allergens
CN104558100A (zh) * 2015-02-04 2015-04-29 吉林农业大学 包涵体的预处理方法

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0596459A2 (en) * 1986-02-03 1994-05-11 Cambridge Biotech Corporation Process for purifying recombinant proteins, and use of products thereof
RU2075509C1 (ru) * 1989-06-12 1997-03-20 Питман-Мур, Инк. Способ выделения рекомбинантного соматотропина

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK0432419T3 (da) 1989-12-05 1994-11-14 American Cyanamid Co Fremgangsmåde til solubilisering og naturering af somatotropiner under anvendelse af lav urinstofkoncentration
US5912327A (en) * 1996-09-30 1999-06-15 Human Genome Sciences, Inc. Method of purifying chemokines from inclusion bodies
SE9703531D0 (sv) * 1997-09-30 1997-09-30 Rudolf Valenta Non-anaphlactic forms of allergens and their use

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0596459A2 (en) * 1986-02-03 1994-05-11 Cambridge Biotech Corporation Process for purifying recombinant proteins, and use of products thereof
RU2075509C1 (ru) * 1989-06-12 1997-03-20 Питман-Мур, Инк. Способ выделения рекомбинантного соматотропина

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
К.HOFFMANN-SOMMERGRUBER ЕТ AL. High-level expression and purification of the major birch pollen allergen. Bet v 1. Protein expression and purification, 1999, 9, 33-39. *

Also Published As

Publication number Publication date
JP2004508384A (ja) 2004-03-18
EP1315740B1 (de) 2009-09-23
DE50115119D1 (de) 2009-11-05
CA2421438C (en) 2010-10-26
AU2001287695B2 (en) 2006-10-05
DE10044360A1 (de) 2002-03-21
CN1468255A (zh) 2004-01-14
US20030170815A1 (en) 2003-09-11
WO2002020559A1 (de) 2002-03-14
EP1315740A1 (de) 2003-06-04
CA2421438A1 (en) 2003-03-06
PL206158B1 (pl) 2010-07-30
JP5174312B2 (ja) 2013-04-03
US6900034B2 (en) 2005-05-31
ES2333939T3 (es) 2010-03-03
AU8769501A (en) 2002-03-22
PT1315740E (pt) 2009-12-29
PL359713A1 (en) 2004-09-06
ATE443712T1 (de) 2009-10-15
HUP0303554A2 (hu) 2004-03-01

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MM4A The patent is invalid due to non-payment of fees

Effective date: 20170819