PL206158B1 - Sposób otrzymywania oczyszczonych, rekombinowanych wariantów alergenu Bet v 1 - Google Patents
Sposób otrzymywania oczyszczonych, rekombinowanych wariantów alergenu Bet v 1Info
- Publication number
- PL206158B1 PL206158B1 PL359713A PL35971301A PL206158B1 PL 206158 B1 PL206158 B1 PL 206158B1 PL 359713 A PL359713 A PL 359713A PL 35971301 A PL35971301 A PL 35971301A PL 206158 B1 PL206158 B1 PL 206158B1
- Authority
- PL
- Poland
- Prior art keywords
- purification
- protein
- recombinant
- solution
- chromatographic
- Prior art date
Links
- 238000000034 method Methods 0.000 title claims abstract description 57
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 title claims abstract description 21
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 title claims abstract description 21
- 239000013566 allergen Substances 0.000 claims abstract description 50
- 238000000746 purification Methods 0.000 claims abstract description 26
- 150000003839 salts Chemical class 0.000 claims abstract description 24
- 238000004587 chromatography analysis Methods 0.000 claims abstract description 21
- 239000012634 fragment Substances 0.000 claims abstract description 20
- 238000006386 neutralization reaction Methods 0.000 claims abstract description 12
- 230000001580 bacterial effect Effects 0.000 claims abstract description 11
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 10
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 90
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 claims description 48
- 239000000243 solution Substances 0.000 claims description 44
- 102000004169 proteins and genes Human genes 0.000 claims description 40
- 108090000623 proteins and genes Proteins 0.000 claims description 40
- 235000017557 sodium bicarbonate Nutrition 0.000 claims description 24
- 229910000030 sodium bicarbonate Inorganic materials 0.000 claims description 24
- 239000003480 eluent Substances 0.000 claims description 23
- 238000002523 gelfiltration Methods 0.000 claims description 14
- 238000002360 preparation method Methods 0.000 claims description 12
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims description 9
- 239000004202 carbamide Substances 0.000 claims description 9
- 230000002209 hydrophobic effect Effects 0.000 claims description 9
- 230000002452 interceptive effect Effects 0.000 claims description 9
- 230000000694 effects Effects 0.000 claims description 8
- 150000001413 amino acids Chemical class 0.000 claims description 7
- 239000000463 material Substances 0.000 claims description 6
- 239000013638 trimer Substances 0.000 claims description 6
- 239000012670 alkaline solution Substances 0.000 claims description 5
- 150000001450 anions Chemical class 0.000 claims description 5
- 238000011210 chromatographic step Methods 0.000 claims description 5
- 239000000872 buffer Substances 0.000 claims description 4
- 238000011097 chromatography purification Methods 0.000 claims description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 3
- 230000003321 amplification Effects 0.000 claims description 3
- 239000000356 contaminant Substances 0.000 claims description 3
- 229960000789 guanidine hydrochloride Drugs 0.000 claims description 3
- PJJJBBJSCAKJQF-UHFFFAOYSA-N guanidinium chloride Chemical compound [Cl-].NC(N)=[NH2+] PJJJBBJSCAKJQF-UHFFFAOYSA-N 0.000 claims description 3
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 3
- 210000001744 T-lymphocyte Anatomy 0.000 claims description 2
- 230000003472 neutralizing effect Effects 0.000 claims description 2
- 239000011780 sodium chloride Substances 0.000 claims description 2
- 235000019846 buffering salt Nutrition 0.000 claims 1
- 210000004027 cell Anatomy 0.000 abstract description 7
- 210000003000 inclusion body Anatomy 0.000 abstract description 3
- 239000002904 solvent Substances 0.000 abstract description 3
- 238000006243 chemical reaction Methods 0.000 abstract description 2
- 238000004925 denaturation Methods 0.000 abstract description 2
- 230000036425 denaturation Effects 0.000 abstract description 2
- 230000003381 solubilizing effect Effects 0.000 abstract 1
- 229960004784 allergens Drugs 0.000 description 39
- 206010020751 Hypersensitivity Diseases 0.000 description 16
- 208000026935 allergic disease Diseases 0.000 description 15
- 230000007815 allergy Effects 0.000 description 15
- 239000011734 sodium Substances 0.000 description 6
- 239000000203 mixture Substances 0.000 description 5
- 239000000047 product Substances 0.000 description 5
- 230000005526 G1 to G0 transition Effects 0.000 description 4
- 239000002253 acid Substances 0.000 description 4
- 230000000172 allergic effect Effects 0.000 description 4
- 238000010828 elution Methods 0.000 description 4
- 238000004191 hydrophobic interaction chromatography Methods 0.000 description 4
- 239000012535 impurity Substances 0.000 description 4
- 150000002500 ions Chemical class 0.000 description 4
- 238000004153 renaturation Methods 0.000 description 4
- 238000000926 separation method Methods 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 238000002560 therapeutic procedure Methods 0.000 description 4
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 3
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 description 3
- 239000007983 Tris buffer Substances 0.000 description 3
- 239000013575 birch pollen allergen Substances 0.000 description 3
- 239000003795 chemical substances by application Substances 0.000 description 3
- 238000003745 diagnosis Methods 0.000 description 3
- 238000000502 dialysis Methods 0.000 description 3
- 239000012467 final product Substances 0.000 description 3
- 238000009169 immunotherapy Methods 0.000 description 3
- 238000012856 packing Methods 0.000 description 3
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 3
- 241000238631 Hexapoda Species 0.000 description 2
- NTYJJOPFIAHURM-UHFFFAOYSA-N Histamine Chemical compound NCCC1=CN=CN1 NTYJJOPFIAHURM-UHFFFAOYSA-N 0.000 description 2
- 239000012505 Superdex™ Substances 0.000 description 2
- 239000013543 active substance Substances 0.000 description 2
- 230000002411 adverse Effects 0.000 description 2
- 208000010668 atopic eczema Diseases 0.000 description 2
- 230000004071 biological effect Effects 0.000 description 2
- 238000011109 contamination Methods 0.000 description 2
- 238000011026 diafiltration Methods 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 238000003780 insertion Methods 0.000 description 2
- 230000037431 insertion Effects 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 230000000144 pharmacologic effect Effects 0.000 description 2
- 229920001184 polypeptide Polymers 0.000 description 2
- 102000004196 processed proteins & peptides Human genes 0.000 description 2
- 108090000765 processed proteins & peptides Proteins 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 229910052708 sodium Inorganic materials 0.000 description 2
- 238000001228 spectrum Methods 0.000 description 2
- 241000238876 Acari Species 0.000 description 1
- BVKZGUZCCUSVTD-UHFFFAOYSA-M Bicarbonate Chemical compound OC([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-M 0.000 description 1
- 108091028026 C-DNA Proteins 0.000 description 1
- 241000282472 Canis lupus familiaris Species 0.000 description 1
- 206010010744 Conjunctivitis allergic Diseases 0.000 description 1
- 102000004127 Cytokines Human genes 0.000 description 1
- 108090000695 Cytokines Proteins 0.000 description 1
- 241000283086 Equidae Species 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- 241000282326 Felis catus Species 0.000 description 1
- 102000003886 Glycoproteins Human genes 0.000 description 1
- 108090000288 Glycoproteins Proteins 0.000 description 1
- 108010003272 Hyaluronate lyase Proteins 0.000 description 1
- 102000001974 Hyaluronidases Human genes 0.000 description 1
- 241000257303 Hymenoptera Species 0.000 description 1
- 102100037611 Lysophospholipase Human genes 0.000 description 1
- 102000043276 Oncogene Human genes 0.000 description 1
- 108700020796 Oncogene Proteins 0.000 description 1
- 108010058864 Phospholipases A2 Proteins 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 206010039085 Rhinitis allergic Diseases 0.000 description 1
- 241000256856 Vespidae Species 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 230000001154 acute effect Effects 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 239000013567 aeroallergen Substances 0.000 description 1
- 239000013572 airborne allergen Substances 0.000 description 1
- 230000002009 allergenic effect Effects 0.000 description 1
- 208000002205 allergic conjunctivitis Diseases 0.000 description 1
- 208000030961 allergic reaction Diseases 0.000 description 1
- 201000010105 allergic rhinitis Diseases 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 208000006673 asthma Diseases 0.000 description 1
- 208000024998 atopic conjunctivitis Diseases 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000003139 buffering effect Effects 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 238000013375 chromatographic separation Methods 0.000 description 1
- 239000012501 chromatography medium Substances 0.000 description 1
- XLJMAIOERFSOGZ-UHFFFAOYSA-M cyanate Chemical compound [O-]C#N XLJMAIOERFSOGZ-UHFFFAOYSA-M 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 238000002405 diagnostic procedure Methods 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 239000000539 dimer Substances 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 239000012636 effector Substances 0.000 description 1
- 239000002158 endotoxin Substances 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 238000011049 filling Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- -1 for example Substances 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 238000007429 general method Methods 0.000 description 1
- 239000013574 grass pollen allergen Substances 0.000 description 1
- 210000002443 helper t lymphocyte Anatomy 0.000 description 1
- 229960001340 histamine Drugs 0.000 description 1
- 210000003630 histaminocyte Anatomy 0.000 description 1
- 229960002773 hyaluronidase Drugs 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 208000030603 inherited susceptibility to asthma Diseases 0.000 description 1
- 238000005342 ion exchange Methods 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 239000002655 kraft paper Substances 0.000 description 1
- 230000005923 long-lasting effect Effects 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 210000004400 mucous membrane Anatomy 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 229940094443 oxytocics prostaglandins Drugs 0.000 description 1
- 238000001139 pH measurement Methods 0.000 description 1
- 238000005192 partition Methods 0.000 description 1
- 239000000825 pharmaceutical preparation Substances 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 230000007096 poisonous effect Effects 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 239000011736 potassium bicarbonate Substances 0.000 description 1
- 235000015497 potassium bicarbonate Nutrition 0.000 description 1
- 229910000028 potassium bicarbonate Inorganic materials 0.000 description 1
- 150000003112 potassium compounds Chemical class 0.000 description 1
- TYJJADVDDVDEDZ-UHFFFAOYSA-M potassium hydrogencarbonate Chemical compound [K+].OC([O-])=O TYJJADVDDVDEDZ-UHFFFAOYSA-M 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 150000003180 prostaglandins Chemical class 0.000 description 1
- 238000001742 protein purification Methods 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000001226 reprecipitation Methods 0.000 description 1
- 239000012488 sample solution Substances 0.000 description 1
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 239000011550 stock solution Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 230000001131 transforming effect Effects 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
- C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
- C07K1/1136—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by reversible modification of the secondary, tertiary or quarternary structure, e.g. using denaturating or stabilising agents
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Analytical Chemistry (AREA)
- Gastroenterology & Hepatology (AREA)
- Botany (AREA)
- Crystallography & Structural Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Peptides Or Proteins (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE10044360A DE10044360A1 (de) | 2000-09-08 | 2000-09-08 | Verfahren zur Aufreinigung von rekombinanten als unlösliche Aggregate exprimierte Proteinen |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| PL359713A1 PL359713A1 (en) | 2004-09-06 |
| PL206158B1 true PL206158B1 (pl) | 2010-07-30 |
Family
ID=7655449
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PL359713A PL206158B1 (pl) | 2000-09-08 | 2001-08-18 | Sposób otrzymywania oczyszczonych, rekombinowanych wariantów alergenu Bet v 1 |
Country Status (14)
| Country | Link |
|---|---|
| US (1) | US6900034B2 (https=) |
| EP (1) | EP1315740B1 (https=) |
| JP (1) | JP5174312B2 (https=) |
| CN (1) | CN1468255A (https=) |
| AT (1) | ATE443712T1 (https=) |
| AU (2) | AU2001287695B2 (https=) |
| CA (1) | CA2421438C (https=) |
| DE (2) | DE10044360A1 (https=) |
| ES (1) | ES2333939T3 (https=) |
| HU (1) | HUP0303554A2 (https=) |
| PL (1) | PL206158B1 (https=) |
| PT (1) | PT1315740E (https=) |
| RU (1) | RU2299214C2 (https=) |
| WO (1) | WO2002020559A1 (https=) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2333951T5 (es) * | 2002-02-27 | 2017-11-16 | Merck Patent Gmbh | Método para producir alergenos principales rBet v 1 hipoalergénicos de abedul |
| CA2487673C (en) * | 2003-12-02 | 2010-11-02 | F. Hoffmann-La Roche Ag | Improved method for the recombinant production and purification of protein kinases |
| EP2323485B1 (en) * | 2008-08-08 | 2013-10-16 | Janssen Pharmaceutica, N.V. | Anti-allergen combinations of calcium and lanthanum salts |
| PL2721051T3 (pl) | 2011-06-15 | 2018-05-30 | Asit Biotech S.A. | Sposób wytwarzania hydrolizowanych alergenów |
| CN104558100A (zh) * | 2015-02-04 | 2015-04-29 | 吉林农业大学 | 包涵体的预处理方法 |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4734362A (en) * | 1986-02-03 | 1988-03-29 | Cambridge Bioscience Corporation | Process for purifying recombinant proteins, and products thereof |
| US5101018A (en) * | 1989-06-12 | 1992-03-31 | International Minerals & Chemical Corp. | Method for recovering recombinant proteins |
| ATE113060T1 (de) | 1989-12-05 | 1994-11-15 | American Cyanamid Co | Methode zur auflösung und naturation von somatotropin unter verwendung von einer niedrigen harnstoffkonzentration. |
| US5912327A (en) | 1996-09-30 | 1999-06-15 | Human Genome Sciences, Inc. | Method of purifying chemokines from inclusion bodies |
| SE9703531D0 (sv) * | 1997-09-30 | 1997-09-30 | Rudolf Valenta | Non-anaphlactic forms of allergens and their use |
-
2000
- 2000-09-08 DE DE10044360A patent/DE10044360A1/de not_active Withdrawn
-
2001
- 2001-08-18 JP JP2002525179A patent/JP5174312B2/ja not_active Expired - Fee Related
- 2001-08-18 AU AU2001287695A patent/AU2001287695B2/en not_active Ceased
- 2001-08-18 AU AU8769501A patent/AU8769501A/xx active Pending
- 2001-08-18 HU HU0303554A patent/HUP0303554A2/hu unknown
- 2001-08-18 CN CNA018153550A patent/CN1468255A/zh active Pending
- 2001-08-18 WO PCT/EP2001/009552 patent/WO2002020559A1/de not_active Ceased
- 2001-08-18 AT AT01967286T patent/ATE443712T1/de active
- 2001-08-18 ES ES01967286T patent/ES2333939T3/es not_active Expired - Lifetime
- 2001-08-18 CA CA2421438A patent/CA2421438C/en not_active Expired - Fee Related
- 2001-08-18 EP EP01967286A patent/EP1315740B1/de not_active Expired - Lifetime
- 2001-08-18 US US10/363,788 patent/US6900034B2/en not_active Expired - Lifetime
- 2001-08-18 RU RU2003109430/13A patent/RU2299214C2/ru not_active IP Right Cessation
- 2001-08-18 PL PL359713A patent/PL206158B1/pl unknown
- 2001-08-18 DE DE50115119T patent/DE50115119D1/de not_active Expired - Lifetime
- 2001-08-18 PT PT01967286T patent/PT1315740E/pt unknown
Also Published As
| Publication number | Publication date |
|---|---|
| DE50115119D1 (de) | 2009-11-05 |
| DE10044360A1 (de) | 2002-03-21 |
| JP2004508384A (ja) | 2004-03-18 |
| JP5174312B2 (ja) | 2013-04-03 |
| EP1315740B1 (de) | 2009-09-23 |
| AU8769501A (en) | 2002-03-22 |
| WO2002020559A1 (de) | 2002-03-14 |
| CA2421438A1 (en) | 2003-03-06 |
| PL359713A1 (en) | 2004-09-06 |
| ATE443712T1 (de) | 2009-10-15 |
| AU2001287695B2 (en) | 2006-10-05 |
| CN1468255A (zh) | 2004-01-14 |
| US20030170815A1 (en) | 2003-09-11 |
| PT1315740E (pt) | 2009-12-29 |
| CA2421438C (en) | 2010-10-26 |
| RU2299214C2 (ru) | 2007-05-20 |
| ES2333939T3 (es) | 2010-03-03 |
| EP1315740A1 (de) | 2003-06-04 |
| HUP0303554A2 (hu) | 2004-03-01 |
| US6900034B2 (en) | 2005-05-31 |
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