AU8769501A - Method for purifying recombinant proteins expressed as insoluble aggregates - Google Patents

Method for purifying recombinant proteins expressed as insoluble aggregates

Info

Publication number
AU8769501A
AU8769501A AU8769501A AU8769501A AU8769501A AU 8769501 A AU8769501 A AU 8769501A AU 8769501 A AU8769501 A AU 8769501A AU 8769501 A AU8769501 A AU 8769501A AU 8769501 A AU8769501 A AU 8769501A
Authority
AU
Australia
Prior art keywords
recombinant proteins
insoluble aggregates
purifying
proteins expressed
purifying recombinant
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
AU8769501A
Other languages
English (en)
Inventor
Roland Suck
Oliver Cromwell
Helmut Fiebig
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Merck Patent GmbH
Original Assignee
Merck Patent GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Merck Patent GmbH filed Critical Merck Patent GmbH
Publication of AU8769501A publication Critical patent/AU8769501A/xx
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
    • C07K1/1136—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by reversible modification of the secondary, tertiary or quarternary structure, e.g. using denaturating or stabilising agents
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Botany (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
AU8769501A 2000-09-08 2001-08-18 Method for purifying recombinant proteins expressed as insoluble aggregates Pending AU8769501A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10044360A DE10044360A1 (de) 2000-09-08 2000-09-08 Verfahren zur Aufreinigung von rekombinanten als unlösliche Aggregate exprimierte Proteinen
PCT/EP2001/009552 WO2002020559A1 (de) 2000-09-08 2001-08-18 Verfahren zur aufreinigung von rekombinanten als unlösliche aggregate exprimierte proteinen

Publications (1)

Publication Number Publication Date
AU8769501A true AU8769501A (en) 2002-03-22

Family

ID=7655449

Family Applications (2)

Application Number Title Priority Date Filing Date
AU8769501A Pending AU8769501A (en) 2000-09-08 2001-08-18 Method for purifying recombinant proteins expressed as insoluble aggregates
AU2001287695A Ceased AU2001287695B2 (en) 2000-09-08 2001-08-18 Method for purifying recombinant proteins expressed as insoluble aggregates

Family Applications After (1)

Application Number Title Priority Date Filing Date
AU2001287695A Ceased AU2001287695B2 (en) 2000-09-08 2001-08-18 Method for purifying recombinant proteins expressed as insoluble aggregates

Country Status (14)

Country Link
US (1) US6900034B2 (https=)
EP (1) EP1315740B1 (https=)
JP (1) JP5174312B2 (https=)
CN (1) CN1468255A (https=)
AT (1) ATE443712T1 (https=)
AU (2) AU8769501A (https=)
CA (1) CA2421438C (https=)
DE (2) DE10044360A1 (https=)
ES (1) ES2333939T3 (https=)
HU (1) HUP0303554A2 (https=)
PL (1) PL206158B1 (https=)
PT (1) PT1315740E (https=)
RU (1) RU2299214C2 (https=)
WO (1) WO2002020559A1 (https=)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1478662B2 (de) * 2002-02-27 2017-07-05 Merck Patent GmbH Verfahren zur herstellung von hypoallergenem birkenpollenhauptallergen rbet v 1
CA2487673C (en) * 2003-12-02 2010-11-02 F. Hoffmann-La Roche Ag Improved method for the recombinant production and purification of protein kinases
WO2010015697A1 (en) * 2008-08-08 2010-02-11 Janssen Pharmaceutica Nv Anti-allergen combinations of calcium and lanthanum salts
EP2721051B1 (en) 2011-06-15 2017-11-29 ASIT BioTech s.a. A method for the production of hydrolyzed allergens
CN104558100A (zh) * 2015-02-04 2015-04-29 吉林农业大学 包涵体的预处理方法

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4734362A (en) * 1986-02-03 1988-03-29 Cambridge Bioscience Corporation Process for purifying recombinant proteins, and products thereof
US5101018A (en) * 1989-06-12 1992-03-31 International Minerals & Chemical Corp. Method for recovering recombinant proteins
DK0432419T3 (da) 1989-12-05 1994-11-14 American Cyanamid Co Fremgangsmåde til solubilisering og naturering af somatotropiner under anvendelse af lav urinstofkoncentration
US5912327A (en) * 1996-09-30 1999-06-15 Human Genome Sciences, Inc. Method of purifying chemokines from inclusion bodies
SE9703531D0 (sv) * 1997-09-30 1997-09-30 Rudolf Valenta Non-anaphlactic forms of allergens and their use

Also Published As

Publication number Publication date
JP2004508384A (ja) 2004-03-18
EP1315740B1 (de) 2009-09-23
DE50115119D1 (de) 2009-11-05
CA2421438C (en) 2010-10-26
AU2001287695B2 (en) 2006-10-05
DE10044360A1 (de) 2002-03-21
CN1468255A (zh) 2004-01-14
US20030170815A1 (en) 2003-09-11
WO2002020559A1 (de) 2002-03-14
EP1315740A1 (de) 2003-06-04
CA2421438A1 (en) 2003-03-06
RU2299214C2 (ru) 2007-05-20
PL206158B1 (pl) 2010-07-30
JP5174312B2 (ja) 2013-04-03
US6900034B2 (en) 2005-05-31
ES2333939T3 (es) 2010-03-03
PT1315740E (pt) 2009-12-29
PL359713A1 (en) 2004-09-06
ATE443712T1 (de) 2009-10-15
HUP0303554A2 (hu) 2004-03-01

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