AU2001231086A1 - Method of affinity purifying proteins using modified bis-arsenical fluorescein - Google Patents
Method of affinity purifying proteins using modified bis-arsenical fluoresceinInfo
- Publication number
- AU2001231086A1 AU2001231086A1 AU2001231086A AU3108601A AU2001231086A1 AU 2001231086 A1 AU2001231086 A1 AU 2001231086A1 AU 2001231086 A AU2001231086 A AU 2001231086A AU 3108601 A AU3108601 A AU 3108601A AU 2001231086 A1 AU2001231086 A1 AU 2001231086A1
- Authority
- AU
- Australia
- Prior art keywords
- purifying proteins
- modified bis
- affinity purifying
- arsenical
- fluorescein
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 title 1
- 102000004169 proteins and genes Human genes 0.000 title 1
- 108090000623 proteins and genes Proteins 0.000 title 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
- C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic Table
- C07F9/66—Arsenic compounds
- C07F9/70—Organo-arsenic compounds
- C07F9/80—Heterocyclic compounds
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K19/00—Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US17805400P | 2000-01-24 | 2000-01-24 | |
US60178054 | 2000-01-24 | ||
US09502664 | 2000-02-11 | ||
US09/502,664 US6831160B1 (en) | 2000-01-24 | 2000-02-11 | Method of affinity purifying proteins using modified bis-arsenical fluorescein |
PCT/US2001/002214 WO2001053325A2 (en) | 2000-01-24 | 2001-01-22 | Method of affinity purifying proteins using modified bis-arsenical fluorescein |
Publications (1)
Publication Number | Publication Date |
---|---|
AU2001231086A1 true AU2001231086A1 (en) | 2001-07-31 |
Family
ID=26873909
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AU2001231086A Abandoned AU2001231086A1 (en) | 2000-01-24 | 2001-01-22 | Method of affinity purifying proteins using modified bis-arsenical fluorescein |
Country Status (3)
Country | Link |
---|---|
US (2) | US6831160B1 (en) |
AU (1) | AU2001231086A1 (en) |
WO (1) | WO2001053325A2 (en) |
Families Citing this family (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7198924B2 (en) | 2000-12-11 | 2007-04-03 | Invitrogen Corporation | Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites |
US7223851B2 (en) | 2003-02-06 | 2007-05-29 | General Dynamics Advanced Information Systems, Inc. | Nucleic acid-binding polymers |
EP1697534B1 (en) | 2003-12-01 | 2010-06-02 | Life Technologies Corporation | Nucleic acid molecules containing recombination sites and methods of using the same |
EP1760092A1 (en) * | 2005-08-26 | 2007-03-07 | Applied Research Systems ARS Holding N.V. | System for screening cells for high expression of a protein of interest |
US7794963B2 (en) | 2007-11-02 | 2010-09-14 | E.I. Du Pont De Nemours And Company | Use of tetracysteine tags in fluorescence-activated cell sorting analysis of prokaryotic cells producing peptides or proteins |
PL2220107T3 (en) | 2007-11-12 | 2017-05-31 | Chreto Aps | Dual affinity polypeptides for purification |
US8647829B2 (en) * | 2009-02-20 | 2014-02-11 | General Electric Company | Switchable affinity binders |
CN102421794B (en) | 2009-05-07 | 2015-05-13 | 科雷托公司 | Method for purification of target polypeptides |
JP5476504B2 (en) * | 2010-03-19 | 2014-04-23 | エスアールアイ インターナショナル | Arsenic Fluorescent Agents and Assays |
WO2013076159A1 (en) * | 2011-11-22 | 2013-05-30 | Imec | Biarsenical nanoparticles for in vivo labeling |
KR20240043838A (en) * | 2022-09-27 | 2024-04-04 | 크리포 주식회사 | A fusion protein that forms self-assembly comprising an intrinsically disordered peptide tag in which major amino acids are composed of charged and hydrophobic amino acids, and a method for purifying recombinant protein using the same |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5264557A (en) | 1991-08-23 | 1993-11-23 | The United States Of America As Represented By The Department Of Health And Human Services | Polypeptide of a human cripto-related gene, CR-3 |
US5415999A (en) | 1993-07-09 | 1995-05-16 | Biocircuits Corporation | Fluorescent lipid polymer-macromolecular ligand compositions as detection element in ligand assays |
US5932474A (en) | 1997-10-21 | 1999-08-03 | The Regents Of The University Of California | Target sequences for synthetic molecules |
US6008378A (en) * | 1997-10-21 | 1999-12-28 | The Regents Of The University Of California | Synthetic molecules that specifically react with target sequences |
-
2000
- 2000-02-11 US US09/502,664 patent/US6831160B1/en not_active Expired - Fee Related
-
2001
- 2001-01-22 AU AU2001231086A patent/AU2001231086A1/en not_active Abandoned
- 2001-01-22 WO PCT/US2001/002214 patent/WO2001053325A2/en active Application Filing
-
2004
- 2004-12-14 US US11/012,853 patent/US20050233428A1/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
US20050233428A1 (en) | 2005-10-20 |
US6831160B1 (en) | 2004-12-14 |
WO2001053325A2 (en) | 2001-07-26 |
WO2001053325A3 (en) | 2002-03-07 |
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