CN1468255A - 表达为不溶性聚集体的重组蛋白质的纯化方法 - Google Patents

表达为不溶性聚集体的重组蛋白质的纯化方法 Download PDF

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Publication number
CN1468255A
CN1468255A CNA018153550A CN01815355A CN1468255A CN 1468255 A CN1468255 A CN 1468255A CN A018153550 A CNA018153550 A CN A018153550A CN 01815355 A CN01815355 A CN 01815355A CN 1468255 A CN1468255 A CN 1468255A
Authority
CN
China
Prior art keywords
anaphylactogen
protein
purifying
variant
nacl
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CNA018153550A
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English (en)
Chinese (zh)
Inventor
R���տ�
R·苏克
ķ����ά
O·克罗姆维尔
H·费比
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Merck Patent GmbH
Original Assignee
Merck Patent GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Merck Patent GmbH filed Critical Merck Patent GmbH
Publication of CN1468255A publication Critical patent/CN1468255A/zh
Pending legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
    • C07K1/1136—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by reversible modification of the secondary, tertiary or quarternary structure, e.g. using denaturating or stabilising agents
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Botany (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CNA018153550A 2000-09-08 2001-08-18 表达为不溶性聚集体的重组蛋白质的纯化方法 Pending CN1468255A (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10044360.5 2000-09-08
DE10044360A DE10044360A1 (de) 2000-09-08 2000-09-08 Verfahren zur Aufreinigung von rekombinanten als unlösliche Aggregate exprimierte Proteinen

Publications (1)

Publication Number Publication Date
CN1468255A true CN1468255A (zh) 2004-01-14

Family

ID=7655449

Family Applications (1)

Application Number Title Priority Date Filing Date
CNA018153550A Pending CN1468255A (zh) 2000-09-08 2001-08-18 表达为不溶性聚集体的重组蛋白质的纯化方法

Country Status (14)

Country Link
US (1) US6900034B2 (https=)
EP (1) EP1315740B1 (https=)
JP (1) JP5174312B2 (https=)
CN (1) CN1468255A (https=)
AT (1) ATE443712T1 (https=)
AU (2) AU2001287695B2 (https=)
CA (1) CA2421438C (https=)
DE (2) DE10044360A1 (https=)
ES (1) ES2333939T3 (https=)
HU (1) HUP0303554A2 (https=)
PL (1) PL206158B1 (https=)
PT (1) PT1315740E (https=)
RU (1) RU2299214C2 (https=)
WO (1) WO2002020559A1 (https=)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102112000B (zh) * 2008-08-08 2015-02-25 詹森药业有限公司 钙盐及镧盐的抗过敏组合
CN104558100A (zh) * 2015-02-04 2015-04-29 吉林农业大学 包涵体的预处理方法

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2333951T5 (es) * 2002-02-27 2017-11-16 Merck Patent Gmbh Método para producir alergenos principales rBet v 1 hipoalergénicos de abedul
CA2487673C (en) * 2003-12-02 2010-11-02 F. Hoffmann-La Roche Ag Improved method for the recombinant production and purification of protein kinases
PL2721051T3 (pl) 2011-06-15 2018-05-30 Asit Biotech S.A. Sposób wytwarzania hydrolizowanych alergenów

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4734362A (en) * 1986-02-03 1988-03-29 Cambridge Bioscience Corporation Process for purifying recombinant proteins, and products thereof
US5101018A (en) * 1989-06-12 1992-03-31 International Minerals & Chemical Corp. Method for recovering recombinant proteins
ATE113060T1 (de) 1989-12-05 1994-11-15 American Cyanamid Co Methode zur auflösung und naturation von somatotropin unter verwendung von einer niedrigen harnstoffkonzentration.
US5912327A (en) 1996-09-30 1999-06-15 Human Genome Sciences, Inc. Method of purifying chemokines from inclusion bodies
SE9703531D0 (sv) * 1997-09-30 1997-09-30 Rudolf Valenta Non-anaphlactic forms of allergens and their use

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102112000B (zh) * 2008-08-08 2015-02-25 詹森药业有限公司 钙盐及镧盐的抗过敏组合
CN104558100A (zh) * 2015-02-04 2015-04-29 吉林农业大学 包涵体的预处理方法

Also Published As

Publication number Publication date
DE50115119D1 (de) 2009-11-05
DE10044360A1 (de) 2002-03-21
JP2004508384A (ja) 2004-03-18
JP5174312B2 (ja) 2013-04-03
EP1315740B1 (de) 2009-09-23
AU8769501A (en) 2002-03-22
WO2002020559A1 (de) 2002-03-14
CA2421438A1 (en) 2003-03-06
PL359713A1 (en) 2004-09-06
ATE443712T1 (de) 2009-10-15
AU2001287695B2 (en) 2006-10-05
US20030170815A1 (en) 2003-09-11
PT1315740E (pt) 2009-12-29
CA2421438C (en) 2010-10-26
RU2299214C2 (ru) 2007-05-20
ES2333939T3 (es) 2010-03-03
EP1315740A1 (de) 2003-06-04
HUP0303554A2 (hu) 2004-03-01
US6900034B2 (en) 2005-05-31
PL206158B1 (pl) 2010-07-30

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