PT2459702T - Meio de cultura de células para a expressão da proteína adamts - Google Patents

Meio de cultura de células para a expressão da proteína adamts

Info

Publication number
PT2459702T
PT2459702T PT107408106T PT10740810T PT2459702T PT 2459702 T PT2459702 T PT 2459702T PT 107408106 T PT107408106 T PT 107408106T PT 10740810 T PT10740810 T PT 10740810T PT 2459702 T PT2459702 T PT 2459702T
Authority
PT
Portugal
Prior art keywords
culture medium
cell culture
protein expression
adamts protein
adamts
Prior art date
Application number
PT107408106T
Other languages
English (en)
Portuguese (pt)
Inventor
Grillberger Leopold
Spenger Alexandra
Hasslacher Meinhard
Grillberger Rana
Reiter Manfred
Original Assignee
Baxalta Inc
Baxalta GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Baxalta Inc, Baxalta GmbH filed Critical Baxalta Inc
Publication of PT2459702T publication Critical patent/PT2459702T/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6489—Metalloendopeptidases (3.4.24)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0681—Cells of the genital tract; Non-germinal cells from gonads
    • C12N5/0682—Cells of the female genital tract, e.g. endometrium; Non-germinal cells from ovaries, e.g. ovarian follicle cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0684—Cells of the urinary tract or kidneys
    • C12N5/0686—Kidney cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/24—Metalloendopeptidases (3.4.24)
    • C12Y304/24087—ADAMTS13 endopeptidase (3.4.24.87)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/12—Light metals, i.e. alkali, alkaline earth, Be, Al, Mg
    • C12N2500/14—Calcium; Ca chelators; Calcitonin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C12N2500/22—Zinc; Zn chelators
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/38—Vitamins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
    • C12N2500/95—Protein-free medium and culture conditions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/99—Serum-free medium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Urology & Nephrology (AREA)
  • Reproductive Health (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
PT107408106T 2009-07-31 2010-07-30 Meio de cultura de células para a expressão da proteína adamts PT2459702T (pt)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US23047709P 2009-07-31 2009-07-31

Publications (1)

Publication Number Publication Date
PT2459702T true PT2459702T (pt) 2016-08-02

Family

ID=42668009

Family Applications (1)

Application Number Title Priority Date Filing Date
PT107408106T PT2459702T (pt) 2009-07-31 2010-07-30 Meio de cultura de células para a expressão da proteína adamts

Country Status (21)

Country Link
US (7) US8313926B2 (enExample)
EP (1) EP2459702B1 (enExample)
JP (5) JP5451884B2 (enExample)
KR (5) KR101808496B1 (enExample)
CN (2) CN102549145B (enExample)
AU (1) AU2010278751B2 (enExample)
BR (2) BR122021005965B1 (enExample)
CA (1) CA2769361A1 (enExample)
CO (1) CO6501191A2 (enExample)
EA (1) EA023193B1 (enExample)
ES (1) ES2583258T3 (enExample)
HR (1) HRP20160783T1 (enExample)
HU (1) HUE029110T2 (enExample)
IN (1) IN2012DN00801A (enExample)
MX (2) MX2012001261A (enExample)
NZ (1) NZ597727A (enExample)
PL (1) PL2459702T3 (enExample)
PT (1) PT2459702T (enExample)
SG (1) SG178194A1 (enExample)
SI (1) SI2459702T1 (enExample)
WO (1) WO2011014838A1 (enExample)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NZ597727A (en) * 2009-07-31 2013-08-30 Baxter Int Cell culture medium for adamts protein expression
EA202090067A3 (ru) * 2010-07-08 2020-07-31 Баксалта Инкорпорейтид Способ получения рекомбинантного adamts13 в культуре клеток
SI2726600T1 (sl) 2011-07-01 2017-07-31 Amgen Inc. Sesalska celična kultura
EP2906683B1 (en) 2012-10-15 2017-05-31 Bristol-Myers Squibb Company Mammalian cell culture processes for protein production
EP2970916B1 (en) 2013-03-13 2021-04-14 Merck Sharp & Dohme Corp. Adapted lepidopteran insect cells for the production of recombinant proteins
US9217168B2 (en) 2013-03-14 2015-12-22 Momenta Pharmaceuticals, Inc. Methods of cell culture
AR095196A1 (es) 2013-03-15 2015-09-30 Regeneron Pharma Medio de cultivo celular libre de suero
KR102418824B1 (ko) * 2013-10-14 2022-07-11 아레스 트레이딩 에스.아. 포유동물의 고성능 유가식 배양을 위한 신규 배지
KR102391259B1 (ko) 2014-06-04 2022-04-26 암젠 인크 포유류 세포 배양물을 회수하는 방법
KR101867134B1 (ko) * 2015-03-23 2018-06-12 한양대학교 산학협력단 포유류 세포를 이용하여 목적 물질을 고효율로 생산하기 위한 세포 배양 배지, 이를 이용한 세포 배양 방법 및 목적 물질의 생산 방법
ES2929531T3 (es) * 2015-05-22 2022-11-30 Dupont Nutrition Biosci Aps Acetolactato descarboxilasa
TW202440903A (zh) 2015-08-04 2024-10-16 美商再生元醫藥公司 補充牛磺酸之細胞培養基及用法(一)
US11191818B2 (en) 2016-08-04 2021-12-07 Takeda Pharmaceutical Company Limited Use of ADAMTS13 for treating, ameliorating and/or preventing vaso-occlusive crisis in sickle cell disease, acute lung injury and/or acute respiratory distress syndrome
PL3535388T3 (pl) * 2016-11-04 2023-08-14 Takeda Pharmaceutical Company Limited Sposoby oczyszczania wirusów związanych z adenowirusami
KR20210084695A (ko) * 2017-07-06 2021-07-07 리제너론 파마슈티칼스 인코포레이티드 당단백질을 만들기 위한 세포 배양 과정
MA52007A (fr) * 2018-03-16 2021-01-20 Genzyme Corp Procédés d'amélioration de la viabilité cellulaire dans un bioréacteur de production
EP3807408A4 (en) * 2018-06-18 2022-03-30 Synthetic Biologics, Inc. METHODS OF MAKING ALKALINE PHOSPHATASE AGENTS
US12661390B2 (en) 2019-10-04 2026-06-23 The Children's Medical Center Corporation ADAMTS13 treatment to enhance graft survival
US12297451B1 (en) 2019-10-25 2025-05-13 Regeneron Pharmaceuticals, Inc. Cell culture medium
IL309560B2 (en) 2019-12-06 2025-05-01 Regeneron Pharma Anti-VEGF protein preparations and methods for their production
EP4127158A2 (en) 2020-04-02 2023-02-08 Takeda Pharmaceutical Company Limited Adamts13 variant, compositions, and uses thereof
MX2022013812A (es) 2020-05-08 2022-12-15 Regeneron Pharma Trampas y mini-trampas de vegf y metodos para el tratamiento de trastornos oculares y cancer.
CN115835904A (zh) 2020-05-22 2023-03-21 武田药品工业有限公司 用于治疗和诊断冠状病毒疾病并发症的adamts13组合物和方法
WO2021246557A1 (ko) * 2020-06-05 2021-12-09 주식회사 프로앱텍 특정한 갯수의 알부민이 연결된 요산산화효소-알부민 복합체 및 이의 제조 방법
CN111808822B (zh) * 2020-07-02 2024-01-12 北京百普赛斯生物科技股份有限公司 细胞培养用补料液及提高重组hek293细胞蛋白表达量的方法

Family Cites Families (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4797368A (en) 1985-03-15 1989-01-10 The United States Of America As Represented By The Department Of Health And Human Services Adeno-associated virus as eukaryotic expression vector
US5139941A (en) 1985-10-31 1992-08-18 University Of Florida Research Foundation, Inc. AAV transduction vectors
US5250421A (en) 1986-01-03 1993-10-05 Genetics Institute, Inc. Method for producing factor VIII:C-type proteins
CZ280901B6 (cs) 1988-10-06 1996-05-15 Hideaki Yamada Způsob kultivace bakterií
JPH0753104B2 (ja) * 1989-02-28 1995-06-07 秀明 山田 細菌の培養法
GB9022545D0 (en) 1990-10-17 1990-11-28 Wellcome Found Culture medium
ATE542891T1 (de) 1994-11-10 2012-02-15 Baxter Healthcare Sa Verfahren zur herstellung von biologischen produkten in protein-freier kultur
CA2211630A1 (en) 1995-02-23 1996-08-29 Quest International B.V. Peptides for tissue and cell culture media
US6013516A (en) 1995-10-06 2000-01-11 The Salk Institute For Biological Studies Vector and method of use for nucleic acid delivery to non-dividing cells
EP2221361A3 (en) 1996-08-30 2011-02-09 Life Technologies Corporation Method for producing a polypeptide in vitro in mammalian cells in a protein-free and serum-free culture medium
IT1285158B1 (it) 1996-09-17 1998-06-03 Angeletti P Ist Richerche Bio Polipeptidi solubili con l'attivita' di serino-proteasi di ns3 del virus dell'epatite c, e procedimento per la loro preparazione e il
UA59375C2 (uk) 1996-10-07 2003-09-15 Солюшіа Інк. Суміш для гідроксилювання, спосіб гідроксилювання ароматичних сполук та спосіб одержання фенолу
US6103529A (en) 1996-10-10 2000-08-15 Life Technologies, Inc. Animal cell culture media comprising peptides derived from rice
US5804420A (en) 1997-04-18 1998-09-08 Bayer Corporation Preparation of recombinant Factor VIII in a protein free medium
AT407255B (de) 1997-06-20 2001-02-26 Immuno Ag Rekombinanter zellklon mit erhöhter stabilität in serum- und proteinfreiem medium und verfahren zur gewinnung des stabilen zellklons
US6475725B1 (en) 1997-06-20 2002-11-05 Baxter Aktiengesellschaft Recombinant cell clones having increased stability and methods of making and using the same
US5994136A (en) 1997-12-12 1999-11-30 Cell Genesys, Inc. Method and means for producing high titer, safe, recombinant lentivirus vectors
US6406909B1 (en) 1998-07-10 2002-06-18 Chugai Seiyaku Kabushiki Kaisha Serum-free medium for culturing animal cells
AT409379B (de) 1999-06-02 2002-07-25 Baxter Ag Medium zur protein- und serumfreien kultivierung von zellen
US6926894B2 (en) * 2000-11-22 2005-08-09 Baxter Aktiengesellschaft Composition exhibiting a von willebrand factor (vWF) protease activity comprising a polypeptide chain with the amino acid sequence AAGGILHLELLV
AU2003295270A1 (en) 2002-12-23 2004-07-14 Fuji Photo Film B.V. Process for coating cell-culture support
MXPA06011815A (es) * 2004-04-16 2006-12-15 Wyeth Corp Proteinas adamts-8 y usos de las mismas.
US20060094104A1 (en) 2004-10-29 2006-05-04 Leopold Grillberger Animal protein-free media for cultivation of cells
JP2007174948A (ja) * 2005-12-27 2007-07-12 Noritsu Koki Co Ltd 酵素水製造装置
JP4880997B2 (ja) * 2005-12-28 2012-02-22 アルフレッサファーマ株式会社 Adamts13の安定化方法
ES2474573T3 (es) * 2006-01-04 2014-07-09 Baxter International Inc Medio de cultivo celular sin oligop�ptidos
BRPI0622214A2 (pt) * 2006-12-22 2012-01-03 Springer Carrier Ltda unidade de condicionamento de ar e mÉtodo de misturar ar em uma unidade de condicionamento de ar
US20090067805A1 (en) 2007-09-10 2009-03-12 Victor Company Of Japan, Limited Recording and reproducing device
SI2574677T1 (sl) * 2007-12-27 2017-11-30 Baxalta GmbH Procesi celične kulture
NZ597727A (en) * 2009-07-31 2013-08-30 Baxter Int Cell culture medium for adamts protein expression

Also Published As

Publication number Publication date
JP2016144471A (ja) 2016-08-12
SG178194A1 (en) 2012-03-29
US20190071660A1 (en) 2019-03-07
JP6567707B2 (ja) 2019-08-28
JP2013500724A (ja) 2013-01-10
US9441216B2 (en) 2016-09-13
US20200308566A1 (en) 2020-10-01
SI2459702T1 (sl) 2016-11-30
US10724024B2 (en) 2020-07-28
AU2010278751A1 (en) 2012-02-09
HK1171790A1 (zh) 2013-04-05
JP5451884B2 (ja) 2014-03-26
MX2012001261A (es) 2012-05-22
JP2019201661A (ja) 2019-11-28
US20140329294A1 (en) 2014-11-06
BR112012002151B1 (pt) 2021-07-06
US8313926B2 (en) 2012-11-20
US10072254B2 (en) 2018-09-11
ES2583258T3 (es) 2016-09-20
MX344970B (es) 2017-01-12
KR20170140411A (ko) 2017-12-20
JP6883071B2 (ja) 2021-06-09
KR101756354B1 (ko) 2017-07-26
AU2010278751B2 (en) 2014-05-15
US20130071906A1 (en) 2013-03-21
CN104962539B (zh) 2019-09-17
US20150329848A1 (en) 2015-11-19
BR122021005965B1 (pt) 2022-01-25
US11254921B2 (en) 2022-02-22
KR20170081747A (ko) 2017-07-12
JP2014061005A (ja) 2014-04-10
CO6501191A2 (es) 2012-08-15
EP2459702B1 (en) 2016-05-04
US8759026B2 (en) 2014-06-24
WO2011014838A1 (en) 2011-02-03
JP6511007B2 (ja) 2019-05-08
PL2459702T3 (pl) 2016-12-30
BR112012002151A2 (pt) 2020-03-03
KR101573972B1 (ko) 2015-12-02
EP2459702A1 (en) 2012-06-06
JP7083059B2 (ja) 2022-06-09
NZ597727A (en) 2013-08-30
KR20150044981A (ko) 2015-04-27
KR101912552B1 (ko) 2018-10-26
JP5936232B2 (ja) 2016-06-22
US9127265B2 (en) 2015-09-08
HUE029110T2 (en) 2017-02-28
JP2021112210A (ja) 2021-08-05
KR20120046301A (ko) 2012-05-09
JP2018093881A (ja) 2018-06-21
CN104962539A (zh) 2015-10-07
HRP20160783T1 (hr) 2016-08-12
EA023193B1 (ru) 2016-05-31
US20110086413A1 (en) 2011-04-14
US20170051270A1 (en) 2017-02-23
CA2769361A1 (en) 2011-02-03
KR101574056B1 (ko) 2015-12-02
CN102549145B (zh) 2015-05-20
KR20150138425A (ko) 2015-12-09
IN2012DN00801A (enExample) 2015-06-26
EA201270224A1 (ru) 2012-09-28
KR101808496B1 (ko) 2017-12-12
CN102549145A (zh) 2012-07-04

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