PT2459702T - Meio de cultura de células para a expressão da proteína adamts - Google Patents

Meio de cultura de células para a expressão da proteína adamts

Info

Publication number
PT2459702T
PT2459702T PT107408106T PT10740810T PT2459702T PT 2459702 T PT2459702 T PT 2459702T PT 107408106 T PT107408106 T PT 107408106T PT 10740810 T PT10740810 T PT 10740810T PT 2459702 T PT2459702 T PT 2459702T
Authority
PT
Portugal
Prior art keywords
culture medium
cell culture
protein expression
adamts protein
adamts
Prior art date
Application number
PT107408106T
Other languages
English (en)
Portuguese (pt)
Inventor
Grillberger Leopold
Spenger Alexandra
Hasslacher Meinhard
Grillberger Rana
Reiter Manfred
Original Assignee
Baxalta Inc
Baxalta GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Baxalta Inc, Baxalta GmbH filed Critical Baxalta Inc
Publication of PT2459702T publication Critical patent/PT2459702T/pt

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/48Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6489Metalloendopeptidases (3.4.24)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0681Cells of the genital tract; Non-germinal cells from gonads
    • C12N5/0682Cells of the female genital tract, e.g. endometrium; Non-germinal cells from ovaries, e.g. ovarian follicle cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0684Cells of the urinary tract or kidneys
    • C12N5/0686Kidney cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y304/00Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/24Metalloendopeptidases (3.4.24)
    • C12Y304/24087ADAMTS13 endopeptidase (3.4.24.87)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00Specific components of cell culture medium
    • C12N2500/05Inorganic components
    • C12N2500/10Metals; Metal chelators
    • C12N2500/12Light metals, i.e. alkali, alkaline earth, Be, Al, Mg
    • C12N2500/14Calcium; Ca chelators; Calcitonin
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00Specific components of cell culture medium
    • C12N2500/05Inorganic components
    • C12N2500/10Metals; Metal chelators
    • C12N2500/20Transition metals
    • C12N2500/22Zinc; Zn chelators
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00Specific components of cell culture medium
    • C12N2500/30Organic components
    • C12N2500/38Vitamins
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00Specific components of cell culture medium
    • C12N2500/90Serum-free medium, which may still contain naturally-sourced components
    • C12N2500/95Protein-free medium and culture conditions
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00Specific components of cell culture medium
    • C12N2500/99Serum-free medium
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00Genetically modified cells
    • C12N2510/02Cells for production

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Urology & Nephrology (AREA)
  • Reproductive Health (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
PT107408106T 2009-07-31 2010-07-30 Meio de cultura de células para a expressão da proteína adamts PT2459702T (pt)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US23047709P 2009-07-31 2009-07-31

Publications (1)

Publication Number Publication Date
PT2459702T true PT2459702T (pt) 2016-08-02

Family

ID=42668009

Family Applications (1)

Application Number Title Priority Date Filing Date
PT107408106T PT2459702T (pt) 2009-07-31 2010-07-30 Meio de cultura de células para a expressão da proteína adamts

Country Status (21)

Country Link
US (7) US8313926B2 (enExample)
EP (1) EP2459702B1 (enExample)
JP (5) JP5451884B2 (enExample)
KR (5) KR101756354B1 (enExample)
CN (2) CN104962539B (enExample)
AU (1) AU2010278751B2 (enExample)
BR (2) BR112012002151B1 (enExample)
CA (1) CA2769361A1 (enExample)
CO (1) CO6501191A2 (enExample)
EA (1) EA023193B1 (enExample)
ES (1) ES2583258T3 (enExample)
HR (1) HRP20160783T1 (enExample)
HU (1) HUE029110T2 (enExample)
IN (1) IN2012DN00801A (enExample)
MX (2) MX2012001261A (enExample)
NZ (1) NZ597727A (enExample)
PL (1) PL2459702T3 (enExample)
PT (1) PT2459702T (enExample)
SG (1) SG178194A1 (enExample)
SI (1) SI2459702T1 (enExample)
WO (1) WO2011014838A1 (enExample)

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US20230201319A1 (en) 2020-05-22 2023-06-29 Takeda Pharmaceutical Company Limited Adamts13 compositions and methods for treating and diagnosing complications of coronavirus disease
US20230020297A1 (en) * 2020-06-05 2023-01-19 Proabtech Co., Ltd. Uox-albumin conjugate with certain numbers of albumin conjugated thereto, and manufacturing method thereof
CN111808822B (zh) * 2020-07-02 2024-01-12 北京百普赛斯生物科技股份有限公司 细胞培养用补料液及提高重组hek293细胞蛋白表达量的方法

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Also Published As

Publication number Publication date
KR101573972B1 (ko) 2015-12-02
CN104962539B (zh) 2019-09-17
JP2013500724A (ja) 2013-01-10
BR112012002151A2 (pt) 2020-03-03
EA023193B1 (ru) 2016-05-31
BR122021005965B1 (pt) 2022-01-25
EP2459702B1 (en) 2016-05-04
US9127265B2 (en) 2015-09-08
JP6567707B2 (ja) 2019-08-28
CN102549145B (zh) 2015-05-20
NZ597727A (en) 2013-08-30
US8759026B2 (en) 2014-06-24
EA201270224A1 (ru) 2012-09-28
JP5451884B2 (ja) 2014-03-26
PL2459702T3 (pl) 2016-12-30
SG178194A1 (en) 2012-03-29
KR20120046301A (ko) 2012-05-09
BR112012002151B1 (pt) 2021-07-06
KR101808496B1 (ko) 2017-12-12
KR101574056B1 (ko) 2015-12-02
AU2010278751A1 (en) 2012-02-09
JP7083059B2 (ja) 2022-06-09
CA2769361A1 (en) 2011-02-03
US20190071660A1 (en) 2019-03-07
JP2016144471A (ja) 2016-08-12
HK1171790A1 (zh) 2013-04-05
US20130071906A1 (en) 2013-03-21
CN102549145A (zh) 2012-07-04
US20150329848A1 (en) 2015-11-19
US20110086413A1 (en) 2011-04-14
JP2019201661A (ja) 2019-11-28
JP6883071B2 (ja) 2021-06-09
WO2011014838A1 (en) 2011-02-03
KR20170081747A (ko) 2017-07-12
MX2012001261A (es) 2012-05-22
US10072254B2 (en) 2018-09-11
KR20150138425A (ko) 2015-12-09
KR101912552B1 (ko) 2018-10-26
JP2014061005A (ja) 2014-04-10
KR101756354B1 (ko) 2017-07-26
JP2018093881A (ja) 2018-06-21
CN104962539A (zh) 2015-10-07
US11254921B2 (en) 2022-02-22
ES2583258T3 (es) 2016-09-20
HUE029110T2 (en) 2017-02-28
JP2021112210A (ja) 2021-08-05
EP2459702A1 (en) 2012-06-06
SI2459702T1 (sl) 2016-11-30
US10724024B2 (en) 2020-07-28
US9441216B2 (en) 2016-09-13
US20200308566A1 (en) 2020-10-01
HRP20160783T1 (hr) 2016-08-12
JP5936232B2 (ja) 2016-06-22
KR20150044981A (ko) 2015-04-27
CO6501191A2 (es) 2012-08-15
JP6511007B2 (ja) 2019-05-08
US8313926B2 (en) 2012-11-20
US20140329294A1 (en) 2014-11-06
AU2010278751B2 (en) 2014-05-15
MX344970B (es) 2017-01-12
IN2012DN00801A (enExample) 2015-06-26
US20170051270A1 (en) 2017-02-23
KR20170140411A (ko) 2017-12-20

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