PT1969125E - Microarns que regulam a proliferação e diferenciação de células musculares - Google Patents
Microarns que regulam a proliferação e diferenciação de células musculares Download PDFInfo
- Publication number
- PT1969125E PT1969125E PT06845222T PT06845222T PT1969125E PT 1969125 E PT1969125 E PT 1969125E PT 06845222 T PT06845222 T PT 06845222T PT 06845222 T PT06845222 T PT 06845222T PT 1969125 E PT1969125 E PT 1969125E
- Authority
- PT
- Portugal
- Prior art keywords
- mir
- quot
- expression
- inhibitor
- mirna
- Prior art date
Links
- 108091070501 miRNA Proteins 0.000 title description 193
- 230000024245 cell differentiation Effects 0.000 title description 11
- 230000004663 cell proliferation Effects 0.000 title description 11
- 210000000663 muscle cell Anatomy 0.000 title description 11
- 210000000107 myocyte Anatomy 0.000 claims abstract description 83
- 238000000034 method Methods 0.000 claims abstract description 73
- 108091028606 miR-1 stem-loop Proteins 0.000 claims description 202
- 108091060568 Mir-133 microRNA precursor family Proteins 0.000 claims description 157
- 108091023685 miR-133 stem-loop Proteins 0.000 claims description 157
- 125000003729 nucleotide group Chemical group 0.000 claims description 102
- 239000002773 nucleotide Substances 0.000 claims description 101
- 150000007523 nucleic acids Chemical class 0.000 claims description 91
- 230000004069 differentiation Effects 0.000 claims description 89
- 102000039446 nucleic acids Human genes 0.000 claims description 70
- 108020004707 nucleic acids Proteins 0.000 claims description 70
- 239000003112 inhibitor Substances 0.000 claims description 63
- 230000035755 proliferation Effects 0.000 claims description 59
- 239000013598 vector Substances 0.000 claims description 35
- 230000000295 complement effect Effects 0.000 claims description 31
- 208000029549 Muscle injury Diseases 0.000 claims description 28
- 238000000338 in vitro Methods 0.000 claims description 22
- 102000040430 polynucleotide Human genes 0.000 claims description 21
- 108091033319 polynucleotide Proteins 0.000 claims description 21
- 239000002157 polynucleotide Substances 0.000 claims description 21
- 230000005030 transcription termination Effects 0.000 claims description 11
- 241000124008 Mammalia Species 0.000 claims description 8
- 208000014674 injury Diseases 0.000 claims description 7
- 230000008733 trauma Effects 0.000 claims description 6
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims description 5
- 208000021642 Muscular disease Diseases 0.000 claims description 5
- 208000013875 Heart injury Diseases 0.000 claims description 4
- 230000003412 degenerative effect Effects 0.000 claims description 4
- 230000003387 muscular Effects 0.000 claims description 4
- 230000002708 enhancing effect Effects 0.000 claims description 3
- 208000029578 Muscle disease Diseases 0.000 claims description 2
- 230000014509 gene expression Effects 0.000 abstract description 227
- 210000004027 cell Anatomy 0.000 abstract description 168
- 239000000203 mixture Substances 0.000 abstract description 20
- 108090000623 proteins and genes Proteins 0.000 description 251
- 239000002679 microRNA Substances 0.000 description 222
- 108091092539 MiR-208 Proteins 0.000 description 135
- 108700011259 MicroRNAs Proteins 0.000 description 131
- 229920002477 rna polymer Polymers 0.000 description 125
- 102000053602 DNA Human genes 0.000 description 72
- 108020004414 DNA Proteins 0.000 description 72
- 230000006870 function Effects 0.000 description 58
- 102000004169 proteins and genes Human genes 0.000 description 57
- 210000002027 skeletal muscle Anatomy 0.000 description 54
- 241000699666 Mus <mouse, genus> Species 0.000 description 53
- 230000001105 regulatory effect Effects 0.000 description 53
- 230000000694 effects Effects 0.000 description 52
- 108090000765 processed proteins & peptides Proteins 0.000 description 50
- 235000018102 proteins Nutrition 0.000 description 50
- 230000035897 transcription Effects 0.000 description 50
- 238000013518 transcription Methods 0.000 description 50
- 102000004196 processed proteins & peptides Human genes 0.000 description 46
- 229920001184 polypeptide Polymers 0.000 description 45
- 210000001519 tissue Anatomy 0.000 description 44
- 210000003205 muscle Anatomy 0.000 description 42
- 238000004458 analytical method Methods 0.000 description 40
- 210000003098 myoblast Anatomy 0.000 description 39
- 210000001057 smooth muscle myoblast Anatomy 0.000 description 39
- 108010042291 Serum Response Factor Proteins 0.000 description 38
- 102100022056 Serum response factor Human genes 0.000 description 37
- 238000011161 development Methods 0.000 description 37
- 230000018109 developmental process Effects 0.000 description 37
- 238000000636 Northern blotting Methods 0.000 description 36
- 230000000747 cardiac effect Effects 0.000 description 36
- 239000005090 green fluorescent protein Substances 0.000 description 36
- 108091028043 Nucleic acid sequence Proteins 0.000 description 35
- 210000004413 cardiac myocyte Anatomy 0.000 description 35
- 239000001963 growth medium Substances 0.000 description 35
- 210000002216 heart Anatomy 0.000 description 34
- 102100021454 Histone deacetylase 4 Human genes 0.000 description 33
- 101710177324 Histone deacetylase 4 Proteins 0.000 description 33
- 238000009396 hybridization Methods 0.000 description 31
- 108020004999 messenger RNA Proteins 0.000 description 31
- 108091036066 Three prime untranslated region Proteins 0.000 description 29
- 230000033228 biological regulation Effects 0.000 description 29
- 108010043121 Green Fluorescent Proteins Proteins 0.000 description 28
- 102000004144 Green Fluorescent Proteins Human genes 0.000 description 28
- 241000282414 Homo sapiens Species 0.000 description 28
- 238000003752 polymerase chain reaction Methods 0.000 description 26
- 150000001413 amino acids Chemical class 0.000 description 24
- 230000005764 inhibitory process Effects 0.000 description 24
- 239000000047 product Substances 0.000 description 24
- 239000012634 fragment Substances 0.000 description 23
- 239000003550 marker Substances 0.000 description 23
- AUYYCJSJGJYCDS-LBPRGKRZSA-N Thyrolar Chemical class IC1=CC(C[C@H](N)C(O)=O)=CC(I)=C1OC1=CC=C(O)C(I)=C1 AUYYCJSJGJYCDS-LBPRGKRZSA-N 0.000 description 22
- 238000009739 binding Methods 0.000 description 22
- 230000001965 increasing effect Effects 0.000 description 22
- 210000004165 myocardium Anatomy 0.000 description 22
- 230000002018 overexpression Effects 0.000 description 22
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 21
- 230000027455 binding Effects 0.000 description 21
- 239000005495 thyroid hormone Substances 0.000 description 21
- 229940036555 thyroid hormone Drugs 0.000 description 21
- 102000040945 Transcription factor Human genes 0.000 description 20
- 108091023040 Transcription factor Proteins 0.000 description 20
- 239000002243 precursor Substances 0.000 description 20
- 239000000523 sample Substances 0.000 description 20
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 19
- 108700019146 Transgenes Proteins 0.000 description 19
- 230000009261 transgenic effect Effects 0.000 description 19
- 108060001084 Luciferase Proteins 0.000 description 18
- 101100351033 Mus musculus Pax7 gene Proteins 0.000 description 18
- 108700008625 Reporter Genes Proteins 0.000 description 18
- 238000012360 testing method Methods 0.000 description 18
- 241001465754 Metazoa Species 0.000 description 17
- 108091034117 Oligonucleotide Proteins 0.000 description 17
- 241000894007 species Species 0.000 description 17
- 210000002257 embryonic structure Anatomy 0.000 description 16
- 239000013604 expression vector Substances 0.000 description 16
- 230000001404 mediated effect Effects 0.000 description 16
- 230000035772 mutation Effects 0.000 description 16
- 230000003828 downregulation Effects 0.000 description 15
- 239000002609 medium Substances 0.000 description 15
- 230000037361 pathway Effects 0.000 description 15
- 102000040650 (ribonucleotides)n+m Human genes 0.000 description 14
- 239000005089 Luciferase Substances 0.000 description 14
- 241000251539 Vertebrata <Metazoa> Species 0.000 description 14
- 230000000692 anti-sense effect Effects 0.000 description 14
- 238000002474 experimental method Methods 0.000 description 14
- 230000008569 process Effects 0.000 description 14
- 230000014616 translation Effects 0.000 description 14
- 108091026890 Coding region Proteins 0.000 description 13
- 230000015572 biosynthetic process Effects 0.000 description 13
- 238000001890 transfection Methods 0.000 description 13
- 238000013519 translation Methods 0.000 description 13
- 230000003827 upregulation Effects 0.000 description 13
- 108090000715 Brain-derived neurotrophic factor Proteins 0.000 description 12
- 210000000349 chromosome Anatomy 0.000 description 12
- 238000003776 cleavage reaction Methods 0.000 description 12
- 210000001161 mammalian embryo Anatomy 0.000 description 12
- 230000007017 scission Effects 0.000 description 12
- 230000002103 transcriptional effect Effects 0.000 description 12
- 238000005406 washing Methods 0.000 description 12
- 102000004219 Brain-derived neurotrophic factor Human genes 0.000 description 11
- 102000004190 Enzymes Human genes 0.000 description 11
- 108090000790 Enzymes Proteins 0.000 description 11
- 102000005604 Myosin Heavy Chains Human genes 0.000 description 11
- 229940077737 brain-derived neurotrophic factor Drugs 0.000 description 11
- 229940088598 enzyme Drugs 0.000 description 11
- 230000002068 genetic effect Effects 0.000 description 11
- 238000001727 in vivo Methods 0.000 description 11
- 101000938422 Homo sapiens Eyes absent homolog 4 Proteins 0.000 description 10
- 241000699670 Mus sp. Species 0.000 description 10
- 108010084498 Myosin Heavy Chains Proteins 0.000 description 10
- 108010029485 Protein Isoforms Proteins 0.000 description 10
- 102000001708 Protein Isoforms Human genes 0.000 description 10
- 241000269370 Xenopus <genus> Species 0.000 description 10
- 125000000539 amino acid group Chemical group 0.000 description 10
- 230000007423 decrease Effects 0.000 description 10
- 238000010186 staining Methods 0.000 description 10
- 238000011830 transgenic mouse model Methods 0.000 description 10
- 108010002947 Connectin Proteins 0.000 description 9
- 102000004726 Connectin Human genes 0.000 description 9
- 241000701022 Cytomegalovirus Species 0.000 description 9
- 102100030910 Eyes absent homolog 4 Human genes 0.000 description 9
- 108700039691 Genetic Promoter Regions Proteins 0.000 description 9
- 241000699660 Mus musculus Species 0.000 description 9
- 108010051583 Ventricular Myosins Proteins 0.000 description 9
- 230000004071 biological effect Effects 0.000 description 9
- 229910052799 carbon Inorganic materials 0.000 description 9
- 230000001419 dependent effect Effects 0.000 description 9
- 208000015181 infectious disease Diseases 0.000 description 9
- 230000003993 interaction Effects 0.000 description 9
- 238000004519 manufacturing process Methods 0.000 description 9
- 230000007246 mechanism Effects 0.000 description 9
- 238000011282 treatment Methods 0.000 description 9
- SGKRLCUYIXIAHR-AKNGSSGZSA-N (4s,4ar,5s,5ar,6r,12ar)-4-(dimethylamino)-1,5,10,11,12a-pentahydroxy-6-methyl-3,12-dioxo-4a,5,5a,6-tetrahydro-4h-tetracene-2-carboxamide Chemical compound C1=CC=C2[C@H](C)[C@@H]([C@H](O)[C@@H]3[C@](C(O)=C(C(N)=O)C(=O)[C@H]3N(C)C)(O)C3=O)C3=C(O)C2=C1O SGKRLCUYIXIAHR-AKNGSSGZSA-N 0.000 description 8
- WOVKYSAHUYNSMH-RRKCRQDMSA-N 5-bromodeoxyuridine Chemical compound C1[C@H](O)[C@@H](CO)O[C@H]1N1C(=O)NC(=O)C(Br)=C1 WOVKYSAHUYNSMH-RRKCRQDMSA-N 0.000 description 8
- 101100518995 Caenorhabditis elegans pax-3 gene Proteins 0.000 description 8
- 108091033380 Coding strand Proteins 0.000 description 8
- 101100518997 Mus musculus Pax3 gene Proteins 0.000 description 8
- 108700029229 Transcriptional Regulatory Elements Proteins 0.000 description 8
- 238000003556 assay Methods 0.000 description 8
- 238000010367 cloning Methods 0.000 description 8
- 229960003722 doxycycline Drugs 0.000 description 8
- 238000012744 immunostaining Methods 0.000 description 8
- 238000002347 injection Methods 0.000 description 8
- 239000007924 injection Substances 0.000 description 8
- 230000000670 limiting effect Effects 0.000 description 8
- 108091063796 miR-206 stem-loop Proteins 0.000 description 8
- 230000001114 myogenic effect Effects 0.000 description 8
- 238000012340 reverse transcriptase PCR Methods 0.000 description 8
- 210000000419 skeletal muscle satellite cell Anatomy 0.000 description 8
- 230000022379 skeletal muscle tissue development Effects 0.000 description 8
- 102000004217 thyroid hormone receptors Human genes 0.000 description 8
- 108090000721 thyroid hormone receptors Proteins 0.000 description 8
- 241000894006 Bacteria Species 0.000 description 7
- 102100024785 Fibroblast growth factor 2 Human genes 0.000 description 7
- 108090000379 Fibroblast growth factor 2 Proteins 0.000 description 7
- 108010056785 Myogenin Proteins 0.000 description 7
- 102100032970 Myogenin Human genes 0.000 description 7
- 108020004459 Small interfering RNA Proteins 0.000 description 7
- 230000031018 biological processes and functions Effects 0.000 description 7
- 230000008859 change Effects 0.000 description 7
- 238000001514 detection method Methods 0.000 description 7
- 239000003623 enhancer Substances 0.000 description 7
- 239000000463 material Substances 0.000 description 7
- 230000004048 modification Effects 0.000 description 7
- 238000012986 modification Methods 0.000 description 7
- 210000001087 myotubule Anatomy 0.000 description 7
- 230000004962 physiological condition Effects 0.000 description 7
- 230000001124 posttranscriptional effect Effects 0.000 description 7
- 239000000243 solution Substances 0.000 description 7
- 238000003786 synthesis reaction Methods 0.000 description 7
- 108091032955 Bacterial small RNA Proteins 0.000 description 6
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 6
- ISAKRJDGNUQOIC-UHFFFAOYSA-N Uracil Chemical compound O=C1C=CNC(=O)N1 ISAKRJDGNUQOIC-UHFFFAOYSA-N 0.000 description 6
- 241000700605 Viruses Species 0.000 description 6
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 6
- 238000013459 approach Methods 0.000 description 6
- 230000008827 biological function Effects 0.000 description 6
- 239000003795 chemical substances by application Substances 0.000 description 6
- 230000006378 damage Effects 0.000 description 6
- 230000007547 defect Effects 0.000 description 6
- 229910052739 hydrogen Inorganic materials 0.000 description 6
- -1 miR-30 Proteins 0.000 description 6
- 238000012545 processing Methods 0.000 description 6
- 230000011664 signaling Effects 0.000 description 6
- 210000002023 somite Anatomy 0.000 description 6
- 230000001225 therapeutic effect Effects 0.000 description 6
- 241000252212 Danio rerio Species 0.000 description 5
- 241000255581 Drosophila <fruit fly, genus> Species 0.000 description 5
- 102000047351 Exportin-5 Human genes 0.000 description 5
- 101000847058 Homo sapiens Exportin-5 Proteins 0.000 description 5
- 101100344903 Homo sapiens MED13 gene Proteins 0.000 description 5
- 108010018650 MEF2 Transcription Factors Proteins 0.000 description 5
- 102100026161 Mediator of RNA polymerase II transcription subunit 13 Human genes 0.000 description 5
- 101100079042 Mus musculus Myef2 gene Proteins 0.000 description 5
- 102000009572 RNA Polymerase II Human genes 0.000 description 5
- 108010009460 RNA Polymerase II Proteins 0.000 description 5
- 102000014450 RNA Polymerase III Human genes 0.000 description 5
- 108010078067 RNA Polymerase III Proteins 0.000 description 5
- 108091081021 Sense strand Proteins 0.000 description 5
- IQFYYKKMVGJFEH-XLPZGREQSA-N Thymidine Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](CO)[C@@H](O)C1 IQFYYKKMVGJFEH-XLPZGREQSA-N 0.000 description 5
- 230000009471 action Effects 0.000 description 5
- 230000004913 activation Effects 0.000 description 5
- 230000008436 biogenesis Effects 0.000 description 5
- 238000006243 chemical reaction Methods 0.000 description 5
- 239000002299 complementary DNA Substances 0.000 description 5
- 150000001875 compounds Chemical class 0.000 description 5
- 230000001186 cumulative effect Effects 0.000 description 5
- 230000004927 fusion Effects 0.000 description 5
- 230000009368 gene silencing by RNA Effects 0.000 description 5
- 210000005003 heart tissue Anatomy 0.000 description 5
- 239000001257 hydrogen Substances 0.000 description 5
- 230000002401 inhibitory effect Effects 0.000 description 5
- 238000011068 loading method Methods 0.000 description 5
- 230000009456 molecular mechanism Effects 0.000 description 5
- 230000030147 nuclear export Effects 0.000 description 5
- 239000013612 plasmid Substances 0.000 description 5
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 5
- 108091007428 primary miRNA Proteins 0.000 description 5
- 210000000130 stem cell Anatomy 0.000 description 5
- 235000000346 sugar Nutrition 0.000 description 5
- 230000008685 targeting Effects 0.000 description 5
- 238000012546 transfer Methods 0.000 description 5
- 241000701161 unidentified adenovirus Species 0.000 description 5
- 241001430294 unidentified retrovirus Species 0.000 description 5
- 101100257696 Caenorhabditis elegans srf-3 gene Proteins 0.000 description 4
- 108010051609 Cardiac Myosins Proteins 0.000 description 4
- 102000013602 Cardiac Myosins Human genes 0.000 description 4
- 102000052052 Casein Kinase II Human genes 0.000 description 4
- 108010010919 Casein Kinase II Proteins 0.000 description 4
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 description 4
- ZHNUHDYFZUAESO-UHFFFAOYSA-N Formamide Chemical compound NC=O ZHNUHDYFZUAESO-UHFFFAOYSA-N 0.000 description 4
- 241000287828 Gallus gallus Species 0.000 description 4
- 102000034615 Glial cell line-derived neurotrophic factor Human genes 0.000 description 4
- 108091010837 Glial cell line-derived neurotrophic factor Proteins 0.000 description 4
- 241000282412 Homo Species 0.000 description 4
- 101100456626 Homo sapiens MEF2A gene Proteins 0.000 description 4
- 241001529936 Murinae Species 0.000 description 4
- 102100021148 Myocyte-specific enhancer factor 2A Human genes 0.000 description 4
- 102100039212 Myocyte-specific enhancer factor 2D Human genes 0.000 description 4
- 102000003505 Myosin Human genes 0.000 description 4
- 108060008487 Myosin Proteins 0.000 description 4
- 108020005497 Nuclear hormone receptor Proteins 0.000 description 4
- 241000700159 Rattus Species 0.000 description 4
- 241000700161 Rattus rattus Species 0.000 description 4
- 101001023030 Toxoplasma gondii Myosin-D Proteins 0.000 description 4
- 238000007792 addition Methods 0.000 description 4
- OIRDTQYFTABQOQ-KQYNXXCUSA-N adenosine Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O OIRDTQYFTABQOQ-KQYNXXCUSA-N 0.000 description 4
- 239000000074 antisense oligonucleotide Substances 0.000 description 4
- 238000012230 antisense oligonucleotides Methods 0.000 description 4
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 4
- 230000022131 cell cycle Effects 0.000 description 4
- 239000003153 chemical reaction reagent Substances 0.000 description 4
- 230000003081 coactivator Effects 0.000 description 4
- DDRJAANPRJIHGJ-UHFFFAOYSA-N creatinine Chemical compound CN1CC(=O)NC1=N DDRJAANPRJIHGJ-UHFFFAOYSA-N 0.000 description 4
- OPTASPLRGRRNAP-UHFFFAOYSA-N cytosine Chemical compound NC=1C=CNC(=O)N=1 OPTASPLRGRRNAP-UHFFFAOYSA-N 0.000 description 4
- 230000003247 decreasing effect Effects 0.000 description 4
- 238000012217 deletion Methods 0.000 description 4
- 230000037430 deletion Effects 0.000 description 4
- 239000012895 dilution Substances 0.000 description 4
- 238000010790 dilution Methods 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 230000012010 growth Effects 0.000 description 4
- 230000009067 heart development Effects 0.000 description 4
- 238000002744 homologous recombination Methods 0.000 description 4
- 230000006801 homologous recombination Effects 0.000 description 4
- 230000001939 inductive effect Effects 0.000 description 4
- 101150072601 lin-14 gene Proteins 0.000 description 4
- 108091053735 lin-4 stem-loop Proteins 0.000 description 4
- 108091032363 lin-4-1 stem-loop Proteins 0.000 description 4
- 108091028008 lin-4-2 stem-loop Proteins 0.000 description 4
- 210000004962 mammalian cell Anatomy 0.000 description 4
- 101150014102 mef-2 gene Proteins 0.000 description 4
- 238000002493 microarray Methods 0.000 description 4
- 230000009756 muscle regeneration Effects 0.000 description 4
- 102000006255 nuclear receptors Human genes 0.000 description 4
- 108020004017 nuclear receptors Proteins 0.000 description 4
- 230000007170 pathology Effects 0.000 description 4
- 230000008488 polyadenylation Effects 0.000 description 4
- 230000008439 repair process Effects 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- 230000004044 response Effects 0.000 description 4
- 125000002652 ribonucleotide group Chemical group 0.000 description 4
- 230000001629 suppression Effects 0.000 description 4
- 230000002123 temporal effect Effects 0.000 description 4
- FWBHETKCLVMNFS-UHFFFAOYSA-N 4',6-Diamino-2-phenylindol Chemical compound C1=CC(C(=N)N)=CC=C1C1=CC2=CC=C(C(N)=N)C=C2N1 FWBHETKCLVMNFS-UHFFFAOYSA-N 0.000 description 3
- 102000007469 Actins Human genes 0.000 description 3
- 108010085238 Actins Proteins 0.000 description 3
- 241000271566 Aves Species 0.000 description 3
- 201000010046 Dilated cardiomyopathy Diseases 0.000 description 3
- 206010013801 Duchenne Muscular Dystrophy Diseases 0.000 description 3
- 102100031780 Endonuclease Human genes 0.000 description 3
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 3
- 102000003964 Histone deacetylase Human genes 0.000 description 3
- 108090000353 Histone deacetylase Proteins 0.000 description 3
- 108091092195 Intron Proteins 0.000 description 3
- 241000829100 Macaca mulatta polyomavirus 1 Species 0.000 description 3
- 108091027766 Mir-143 Proteins 0.000 description 3
- 108010081823 Myocardin Proteins 0.000 description 3
- 102100030217 Myocardin Human genes 0.000 description 3
- 241000283973 Oryctolagus cuniculus Species 0.000 description 3
- 108091000080 Phosphotransferase Proteins 0.000 description 3
- 238000012228 RNA interference-mediated gene silencing Methods 0.000 description 3
- 108091028664 Ribonucleotide Proteins 0.000 description 3
- 108020004566 Transfer RNA Proteins 0.000 description 3
- 241000269368 Xenopus laevis Species 0.000 description 3
- 238000002679 ablation Methods 0.000 description 3
- 238000009825 accumulation Methods 0.000 description 3
- 230000003321 amplification Effects 0.000 description 3
- 230000002238 attenuated effect Effects 0.000 description 3
- 210000004899 c-terminal region Anatomy 0.000 description 3
- 210000003855 cell nucleus Anatomy 0.000 description 3
- 230000001413 cellular effect Effects 0.000 description 3
- 235000013330 chicken meat Nutrition 0.000 description 3
- 230000002759 chromosomal effect Effects 0.000 description 3
- 230000001276 controlling effect Effects 0.000 description 3
- 210000000805 cytoplasm Anatomy 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 230000013020 embryo development Effects 0.000 description 3
- 210000001671 embryonic stem cell Anatomy 0.000 description 3
- 230000001605 fetal effect Effects 0.000 description 3
- 230000007045 gastrulation Effects 0.000 description 3
- 238000012252 genetic analysis Methods 0.000 description 3
- 230000035876 healing Effects 0.000 description 3
- 230000001976 improved effect Effects 0.000 description 3
- 238000002955 isolation Methods 0.000 description 3
- 238000011005 laboratory method Methods 0.000 description 3
- 108091023663 let-7 stem-loop Proteins 0.000 description 3
- 238000003468 luciferase reporter gene assay Methods 0.000 description 3
- 239000011159 matrix material Substances 0.000 description 3
- 230000000394 mitotic effect Effects 0.000 description 3
- 238000010369 molecular cloning Methods 0.000 description 3
- 239000000178 monomer Substances 0.000 description 3
- 230000004070 myogenic differentiation Effects 0.000 description 3
- 239000013642 negative control Substances 0.000 description 3
- 230000001537 neural effect Effects 0.000 description 3
- 238000003199 nucleic acid amplification method Methods 0.000 description 3
- 102000020233 phosphotransferase Human genes 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 102000005962 receptors Human genes 0.000 description 3
- 108020003175 receptors Proteins 0.000 description 3
- 230000009467 reduction Effects 0.000 description 3
- 230000000754 repressing effect Effects 0.000 description 3
- 239000002336 ribonucleotide Substances 0.000 description 3
- 210000002235 sarcomere Anatomy 0.000 description 3
- 238000002864 sequence alignment Methods 0.000 description 3
- 210000002966 serum Anatomy 0.000 description 3
- 238000002560 therapeutic procedure Methods 0.000 description 3
- 230000009452 underexpressoin Effects 0.000 description 3
- 238000011144 upstream manufacturing Methods 0.000 description 3
- 229940035893 uracil Drugs 0.000 description 3
- 238000011179 visual inspection Methods 0.000 description 3
- 108020005345 3' Untranslated Regions Proteins 0.000 description 2
- 244000105975 Antidesma platyphyllum Species 0.000 description 2
- DWRXFEITVBNRMK-UHFFFAOYSA-N Beta-D-1-Arabinofuranosylthymine Natural products O=C1NC(=O)C(C)=CN1C1C(O)C(O)C(CO)O1 DWRXFEITVBNRMK-UHFFFAOYSA-N 0.000 description 2
- 239000002126 C01EB10 - Adenosine Substances 0.000 description 2
- 241000244203 Caenorhabditis elegans Species 0.000 description 2
- 241000282472 Canis lupus familiaris Species 0.000 description 2
- 108020004705 Codon Proteins 0.000 description 2
- 208000002330 Congenital Heart Defects Diseases 0.000 description 2
- 206010056370 Congestive cardiomyopathy Diseases 0.000 description 2
- 241001559589 Cullen Species 0.000 description 2
- 238000000116 DAPI staining Methods 0.000 description 2
- 102000004163 DNA-directed RNA polymerases Human genes 0.000 description 2
- 108090000626 DNA-directed RNA polymerases Proteins 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- 108010042407 Endonucleases Proteins 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 108091029865 Exogenous DNA Proteins 0.000 description 2
- 241000233866 Fungi Species 0.000 description 2
- 102100031181 Glyceraldehyde-3-phosphate dehydrogenase Human genes 0.000 description 2
- NYHBQMYGNKIUIF-UUOKFMHZSA-N Guanosine Chemical compound C1=NC=2C(=O)NC(N)=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O NYHBQMYGNKIUIF-UUOKFMHZSA-N 0.000 description 2
- 102000018251 Hypoxanthine Phosphoribosyltransferase Human genes 0.000 description 2
- 108010091358 Hypoxanthine Phosphoribosyltransferase Proteins 0.000 description 2
- 102100025169 Max-binding protein MNT Human genes 0.000 description 2
- 108091062170 Mir-22 Proteins 0.000 description 2
- 108091093189 Mir-375 Proteins 0.000 description 2
- 101000783356 Naja sputatrix Cytotoxin Proteins 0.000 description 2
- 101710163270 Nuclease Proteins 0.000 description 2
- 108700020796 Oncogene Proteins 0.000 description 2
- 241000282577 Pan troglodytes Species 0.000 description 2
- 108020004511 Recombinant DNA Proteins 0.000 description 2
- 102000003661 Ribonuclease III Human genes 0.000 description 2
- 108010057163 Ribonuclease III Proteins 0.000 description 2
- 241000282849 Ruminantia Species 0.000 description 2
- 108091027967 Small hairpin RNA Proteins 0.000 description 2
- 108091060271 Small temporal RNA Proteins 0.000 description 2
- 241000282887 Suidae Species 0.000 description 2
- 241000282898 Sus scrofa Species 0.000 description 2
- 108091085833 TRAP family Proteins 0.000 description 2
- 239000002253 acid Chemical group 0.000 description 2
- 239000012190 activator Substances 0.000 description 2
- 229960005305 adenosine Drugs 0.000 description 2
- 210000001789 adipocyte Anatomy 0.000 description 2
- 230000019552 anatomical structure morphogenesis Effects 0.000 description 2
- 239000005557 antagonist Substances 0.000 description 2
- 238000003491 array Methods 0.000 description 2
- 210000002469 basement membrane Anatomy 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- IQFYYKKMVGJFEH-UHFFFAOYSA-N beta-L-thymidine Natural products O=C1NC(=O)C(C)=CN1C1OC(CO)C(O)C1 IQFYYKKMVGJFEH-UHFFFAOYSA-N 0.000 description 2
- 230000000903 blocking effect Effects 0.000 description 2
- 230000008308 brain morphogenesis Effects 0.000 description 2
- 239000002340 cardiotoxin Substances 0.000 description 2
- 231100000677 cardiotoxin Toxicity 0.000 description 2
- 230000015556 catabolic process Effects 0.000 description 2
- 238000004113 cell culture Methods 0.000 description 2
- 230000033077 cellular process Effects 0.000 description 2
- 238000000205 computational method Methods 0.000 description 2
- 208000028831 congenital heart disease Diseases 0.000 description 2
- 229940109239 creatinine Drugs 0.000 description 2
- 229940104302 cytosine Drugs 0.000 description 2
- 238000006731 degradation reaction Methods 0.000 description 2
- 239000005547 deoxyribonucleotide Substances 0.000 description 2
- 125000002637 deoxyribonucleotide group Chemical group 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 239000006185 dispersion Substances 0.000 description 2
- 231100000673 dose–response relationship Toxicity 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 238000004520 electroporation Methods 0.000 description 2
- 231100001129 embryonic lethality Toxicity 0.000 description 2
- 238000010195 expression analysis Methods 0.000 description 2
- 239000013613 expression plasmid Substances 0.000 description 2
- 239000000284 extract Substances 0.000 description 2
- 210000003414 extremity Anatomy 0.000 description 2
- 102000034356 gene-regulatory proteins Human genes 0.000 description 2
- 108091006104 gene-regulatory proteins Proteins 0.000 description 2
- 108020004445 glyceraldehyde-3-phosphate dehydrogenase Proteins 0.000 description 2
- UYTPUPDQBNUYGX-UHFFFAOYSA-N guanine Chemical compound O=C1NC(N)=NC2=C1N=CN2 UYTPUPDQBNUYGX-UHFFFAOYSA-N 0.000 description 2
- 235000009424 haa Nutrition 0.000 description 2
- 229940088597 hormone Drugs 0.000 description 2
- 239000005556 hormone Substances 0.000 description 2
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 2
- 238000010874 in vitro model Methods 0.000 description 2
- 238000010348 incorporation Methods 0.000 description 2
- 230000006698 induction Effects 0.000 description 2
- 239000003999 initiator Substances 0.000 description 2
- 230000000977 initiatory effect Effects 0.000 description 2
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 description 2
- 230000003914 insulin secretion Effects 0.000 description 2
- 210000004153 islets of langerhan Anatomy 0.000 description 2
- 108091063478 let-7-1 stem-loop Proteins 0.000 description 2
- 108091049777 let-7-2 stem-loop Proteins 0.000 description 2
- 231100000225 lethality Toxicity 0.000 description 2
- 230000004807 localization Effects 0.000 description 2
- 230000035800 maturation Effects 0.000 description 2
- 210000003716 mesoderm Anatomy 0.000 description 2
- 230000004060 metabolic process Effects 0.000 description 2
- 108091045542 miR-1-2 stem-loop Proteins 0.000 description 2
- 108091070946 miR-128 stem-loop Proteins 0.000 description 2
- 108091047757 miR-133a-1 stem-loop Proteins 0.000 description 2
- 108091057540 miR-133a-2 stem-loop Proteins 0.000 description 2
- 108091087148 miR-20 stem-loop Proteins 0.000 description 2
- 108091066984 miR-20-1 stem-loop Proteins 0.000 description 2
- 108091076199 miR-20-2 stem-loop Proteins 0.000 description 2
- 238000010208 microarray analysis Methods 0.000 description 2
- 230000003278 mimic effect Effects 0.000 description 2
- 230000000877 morphologic effect Effects 0.000 description 2
- 238000010172 mouse model Methods 0.000 description 2
- 238000002703 mutagenesis Methods 0.000 description 2
- 231100000350 mutagenesis Toxicity 0.000 description 2
- 206010028417 myasthenia gravis Diseases 0.000 description 2
- 208000010125 myocardial infarction Diseases 0.000 description 2
- 238000000059 patterning Methods 0.000 description 2
- 150000004713 phosphodiesters Chemical class 0.000 description 2
- 229920000642 polymer Polymers 0.000 description 2
- 230000029279 positive regulation of transcription, DNA-dependent Effects 0.000 description 2
- 230000004481 post-translational protein modification Effects 0.000 description 2
- 150000003212 purines Chemical class 0.000 description 2
- 150000003230 pyrimidines Chemical class 0.000 description 2
- 230000002829 reductive effect Effects 0.000 description 2
- 230000008929 regeneration Effects 0.000 description 2
- 238000011069 regeneration method Methods 0.000 description 2
- 230000023276 regulation of development, heterochronic Effects 0.000 description 2
- 230000022532 regulation of transcription, DNA-dependent Effects 0.000 description 2
- 230000002441 reversible effect Effects 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- 230000019491 signal transduction Effects 0.000 description 2
- 239000002924 silencing RNA Substances 0.000 description 2
- 210000002363 skeletal muscle cell Anatomy 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 230000000392 somatic effect Effects 0.000 description 2
- 238000010561 standard procedure Methods 0.000 description 2
- 238000007619 statistical method Methods 0.000 description 2
- 238000006467 substitution reaction Methods 0.000 description 2
- JJAHTWIKCUJRDK-UHFFFAOYSA-N succinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate Chemical class C1CC(CN2C(C=CC2=O)=O)CCC1C(=O)ON1C(=O)CCC1=O JJAHTWIKCUJRDK-UHFFFAOYSA-N 0.000 description 2
- 230000002194 synthesizing effect Effects 0.000 description 2
- 101150024821 tetO gene Proteins 0.000 description 2
- 229940104230 thymidine Drugs 0.000 description 2
- RWQNBRDOKXIBIV-UHFFFAOYSA-N thymine Chemical compound CC1=CNC(=O)NC1=O RWQNBRDOKXIBIV-UHFFFAOYSA-N 0.000 description 2
- 210000001685 thyroid gland Anatomy 0.000 description 2
- 108091006108 transcriptional coactivators Proteins 0.000 description 2
- 108091006107 transcriptional repressors Proteins 0.000 description 2
- 230000032258 transport Effects 0.000 description 2
- 238000001262 western blot Methods 0.000 description 2
- GZCWLCBFPRFLKL-UHFFFAOYSA-N 1-prop-2-ynoxypropan-2-ol Chemical compound CC(O)COCC#C GZCWLCBFPRFLKL-UHFFFAOYSA-N 0.000 description 1
- NEWKHUASLBMWRE-UHFFFAOYSA-N 2-methyl-6-(phenylethynyl)pyridine Chemical compound CC1=CC=CC(C#CC=2C=CC=CC=2)=N1 NEWKHUASLBMWRE-UHFFFAOYSA-N 0.000 description 1
- OSJPPGNTCRNQQC-UWTATZPHSA-N 3-phospho-D-glyceric acid Chemical compound OC(=O)[C@H](O)COP(O)(O)=O OSJPPGNTCRNQQC-UWTATZPHSA-N 0.000 description 1
- NUKYPUAOHBNCPY-UHFFFAOYSA-N 4-aminopyridine Chemical compound NC1=CC=NC=C1 NUKYPUAOHBNCPY-UHFFFAOYSA-N 0.000 description 1
- 101710169336 5'-deoxyadenosine deaminase Proteins 0.000 description 1
- KDCGOANMDULRCW-UHFFFAOYSA-N 7H-purine Chemical compound N1=CNC2=NC=NC2=C1 KDCGOANMDULRCW-UHFFFAOYSA-N 0.000 description 1
- 108010051457 Acid Phosphatase Proteins 0.000 description 1
- 102000013563 Acid Phosphatase Human genes 0.000 description 1
- 229930024421 Adenine Natural products 0.000 description 1
- GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical compound NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 description 1
- 102100036664 Adenosine deaminase Human genes 0.000 description 1
- 206010001258 Adenoviral infections Diseases 0.000 description 1
- 108010087905 Adenovirus E1B Proteins Proteins 0.000 description 1
- 102100024321 Alkaline phosphatase, placental type Human genes 0.000 description 1
- 108700028369 Alleles Proteins 0.000 description 1
- 241000272814 Anser sp. Species 0.000 description 1
- 241000272517 Anseriformes Species 0.000 description 1
- 108010032595 Antibody Binding Sites Proteins 0.000 description 1
- 108020004519 Antisense Oligoribonucleotides Proteins 0.000 description 1
- 101100257701 Arabidopsis thaliana SRF8 gene Proteins 0.000 description 1
- 241000881665 Argonauta Species 0.000 description 1
- 241000972773 Aulopiformes Species 0.000 description 1
- 102100026189 Beta-galactosidase Human genes 0.000 description 1
- 241000157302 Bison bison athabascae Species 0.000 description 1
- 108010029692 Bisphosphoglycerate mutase Proteins 0.000 description 1
- 241000283690 Bos taurus Species 0.000 description 1
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 1
- 101100181929 Caenorhabditis elegans lin-3 gene Proteins 0.000 description 1
- 101100023365 Caenorhabditis elegans mif-2 gene Proteins 0.000 description 1
- 102100033620 Calponin-1 Human genes 0.000 description 1
- 241000282832 Camelidae Species 0.000 description 1
- 241000282461 Canis lupus Species 0.000 description 1
- 241000283707 Capra Species 0.000 description 1
- 101710132601 Capsid protein Proteins 0.000 description 1
- 208000005623 Carcinogenesis Diseases 0.000 description 1
- 206010007572 Cardiac hypertrophy Diseases 0.000 description 1
- 208000006029 Cardiomegaly Diseases 0.000 description 1
- 241001466804 Carnivora Species 0.000 description 1
- 108010078791 Carrier Proteins Proteins 0.000 description 1
- 102000014914 Carrier Proteins Human genes 0.000 description 1
- 241000282994 Cervidae Species 0.000 description 1
- 108010035563 Chloramphenicol O-acetyltransferase Proteins 0.000 description 1
- 108010077544 Chromatin Proteins 0.000 description 1
- 101710094648 Coat protein Proteins 0.000 description 1
- 102000008186 Collagen Human genes 0.000 description 1
- 108010035532 Collagen Proteins 0.000 description 1
- 108020004635 Complementary DNA Proteins 0.000 description 1
- 108020004394 Complementary RNA Proteins 0.000 description 1
- 241000777300 Congiopodidae Species 0.000 description 1
- 102000002585 Contractile Proteins Human genes 0.000 description 1
- 108010068426 Contractile Proteins Proteins 0.000 description 1
- MIKUYHXYGGJMLM-GIMIYPNGSA-N Crotonoside Natural products C1=NC2=C(N)NC(=O)N=C2N1[C@H]1O[C@@H](CO)[C@H](O)[C@@H]1O MIKUYHXYGGJMLM-GIMIYPNGSA-N 0.000 description 1
- NYHBQMYGNKIUIF-UHFFFAOYSA-N D-guanosine Natural products C1=2NC(N)=NC(=O)C=2N=CN1C1OC(CO)C(O)C1O NYHBQMYGNKIUIF-UHFFFAOYSA-N 0.000 description 1
- 101700024220 DACH2 Proteins 0.000 description 1
- 230000004543 DNA replication Effects 0.000 description 1
- 230000006820 DNA synthesis Effects 0.000 description 1
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 1
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 1
- 102100025694 Dachshund homolog 2 Human genes 0.000 description 1
- 206010011878 Deafness Diseases 0.000 description 1
- 206010011891 Deafness neurosensory Diseases 0.000 description 1
- 101100239693 Dictyostelium discoideum myoD gene Proteins 0.000 description 1
- 102100024746 Dihydrofolate reductase Human genes 0.000 description 1
- 108090000204 Dipeptidase 1 Proteins 0.000 description 1
- 108700001857 Drosophila eya Proteins 0.000 description 1
- 102100023272 Dual specificity mitogen-activated protein kinase kinase 5 Human genes 0.000 description 1
- 241000701959 Escherichia virus Lambda Species 0.000 description 1
- 108090000371 Esterases Proteins 0.000 description 1
- 108060002716 Exonuclease Proteins 0.000 description 1
- 241000282326 Felis catus Species 0.000 description 1
- 229920001917 Ficoll Polymers 0.000 description 1
- 108700028146 Genetic Enhancer Elements Proteins 0.000 description 1
- 241000282818 Giraffidae Species 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 102100021181 Golgi phosphoprotein 3 Human genes 0.000 description 1
- 102000002812 Heat-Shock Proteins Human genes 0.000 description 1
- 108010004889 Heat-Shock Proteins Proteins 0.000 description 1
- 108010068250 Herpes Simplex Virus Protein Vmw65 Proteins 0.000 description 1
- 108090000246 Histone acetyltransferases Proteins 0.000 description 1
- 102000003893 Histone acetyltransferases Human genes 0.000 description 1
- 102100021453 Histone deacetylase 5 Human genes 0.000 description 1
- 102000009331 Homeodomain Proteins Human genes 0.000 description 1
- 108010048671 Homeodomain Proteins Proteins 0.000 description 1
- 101000945318 Homo sapiens Calponin-1 Proteins 0.000 description 1
- 101001115390 Homo sapiens Dual specificity mitogen-activated protein kinase kinase 5 Proteins 0.000 description 1
- 101100388713 Homo sapiens EEF2 gene Proteins 0.000 description 1
- 101000899255 Homo sapiens Histone deacetylase 5 Proteins 0.000 description 1
- 101100456570 Homo sapiens MED12 gene Proteins 0.000 description 1
- 101100236776 Homo sapiens MED13L gene Proteins 0.000 description 1
- 101000582320 Homo sapiens Neurogenic differentiation factor 6 Proteins 0.000 description 1
- 101001059454 Homo sapiens Serine/threonine-protein kinase MARK2 Proteins 0.000 description 1
- 101000824035 Homo sapiens Serum response factor Proteins 0.000 description 1
- 101000819074 Homo sapiens Transcription factor GATA-4 Proteins 0.000 description 1
- 101000652736 Homo sapiens Transgelin Proteins 0.000 description 1
- 102000002265 Human Growth Hormone Human genes 0.000 description 1
- 108010000521 Human Growth Hormone Proteins 0.000 description 1
- 239000000854 Human Growth Hormone Substances 0.000 description 1
- 108090000144 Human Proteins Proteins 0.000 description 1
- 102000003839 Human Proteins Human genes 0.000 description 1
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 1
- 206010020850 Hyperthyroidism Diseases 0.000 description 1
- 102000004877 Insulin Human genes 0.000 description 1
- 102100023915 Insulin Human genes 0.000 description 1
- 108090001061 Insulin Proteins 0.000 description 1
- 102100039062 Interleukin enhancer-binding factor 3 Human genes 0.000 description 1
- 102100029874 Kappa-casein Human genes 0.000 description 1
- 150000008575 L-amino acids Chemical group 0.000 description 1
- ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 description 1
- ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 description 1
- 241001417534 Lutjanidae Species 0.000 description 1
- 108091007775 MIR1-1 Proteins 0.000 description 1
- 101710125418 Major capsid protein Proteins 0.000 description 1
- 102000010904 Mediator Complex Subunit 1 Human genes 0.000 description 1
- 108010062495 Mediator Complex Subunit 1 Proteins 0.000 description 1
- 102100021070 Mediator of RNA polymerase II transcription subunit 12 Human genes 0.000 description 1
- 102100034164 Mediator of RNA polymerase II transcription subunit 13-like Human genes 0.000 description 1
- 108091030146 MiRBase Proteins 0.000 description 1
- 108091028684 Mir-145 Proteins 0.000 description 1
- 241000713869 Moloney murine leukemia virus Species 0.000 description 1
- 101100496106 Mus musculus Clec2f gene Proteins 0.000 description 1
- 101100498160 Mus musculus Dach1 gene Proteins 0.000 description 1
- 101100230655 Mus musculus Hdac4 gene Proteins 0.000 description 1
- 101100025404 Mus musculus Myh6 gene Proteins 0.000 description 1
- 101000824036 Mus musculus Serum response factor Proteins 0.000 description 1
- 102100039229 Myocyte-specific enhancer factor 2C Human genes 0.000 description 1
- 102000036675 Myoglobin Human genes 0.000 description 1
- 108010062374 Myoglobin Proteins 0.000 description 1
- 201000009623 Myopathy Diseases 0.000 description 1
- 206010028643 Myopathy endocrine Diseases 0.000 description 1
- 201000002481 Myositis Diseases 0.000 description 1
- 241000244206 Nematoda Species 0.000 description 1
- 102100030589 Neurogenic differentiation factor 6 Human genes 0.000 description 1
- 101150114527 Nkx2-5 gene Proteins 0.000 description 1
- 108010010424 Nuclear Factor 90 Proteins Proteins 0.000 description 1
- 102000007999 Nuclear Proteins Human genes 0.000 description 1
- 108010089610 Nuclear Proteins Proteins 0.000 description 1
- 108020004711 Nucleic Acid Probes Proteins 0.000 description 1
- 108091005461 Nucleic proteins Chemical group 0.000 description 1
- 101710141454 Nucleoprotein Proteins 0.000 description 1
- 102000017946 PGC-1 Human genes 0.000 description 1
- 108700038399 PGC-1 Proteins 0.000 description 1
- 101150012394 PHO5 gene Proteins 0.000 description 1
- 241000282320 Panthera leo Species 0.000 description 1
- 241001278385 Panthera tigris altaica Species 0.000 description 1
- 241001494479 Pecora Species 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 102000035195 Peptidases Human genes 0.000 description 1
- 108700020962 Peroxidase Proteins 0.000 description 1
- 102000003992 Peroxidases Human genes 0.000 description 1
- 102000003921 Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha Human genes 0.000 description 1
- 108090000310 Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha Proteins 0.000 description 1
- 241000286209 Phasianidae Species 0.000 description 1
- 102000011025 Phosphoglycerate Mutase Human genes 0.000 description 1
- 108700019535 Phosphoprotein Phosphatases Proteins 0.000 description 1
- 102000045595 Phosphoprotein Phosphatases Human genes 0.000 description 1
- 102000009097 Phosphorylases Human genes 0.000 description 1
- 108010073135 Phosphorylases Proteins 0.000 description 1
- 239000004952 Polyamide Substances 0.000 description 1
- 108091000054 Prion Proteins 0.000 description 1
- 102000029797 Prion Human genes 0.000 description 1
- 101710083689 Probable capsid protein Proteins 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 108010001267 Protein Subunits Proteins 0.000 description 1
- 102000002067 Protein Subunits Human genes 0.000 description 1
- CZPWVGJYEJSRLH-UHFFFAOYSA-N Pyrimidine Chemical compound C1=CN=CN=C1 CZPWVGJYEJSRLH-UHFFFAOYSA-N 0.000 description 1
- LCTONWCANYUPML-UHFFFAOYSA-M Pyruvate Chemical compound CC(=O)C([O-])=O LCTONWCANYUPML-UHFFFAOYSA-M 0.000 description 1
- 101710086015 RNA ligase Proteins 0.000 description 1
- 239000013614 RNA sample Substances 0.000 description 1
- 230000006819 RNA synthesis Effects 0.000 description 1
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 description 1
- 108091030071 RNAI Proteins 0.000 description 1
- 238000010240 RT-PCR analysis Methods 0.000 description 1
- 108091027981 Response element Proteins 0.000 description 1
- 108010083644 Ribonucleases Proteins 0.000 description 1
- 102000006382 Ribonucleases Human genes 0.000 description 1
- 108010081734 Ribonucleoproteins Proteins 0.000 description 1
- 102000004389 Ribonucleoproteins Human genes 0.000 description 1
- 206010039203 Road traffic accident Diseases 0.000 description 1
- 230000018199 S phase Effects 0.000 description 1
- 108091006296 SLC2A1 Proteins 0.000 description 1
- 101150067809 SRF gene Proteins 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 208000009966 Sensorineural Hearing Loss Diseases 0.000 description 1
- 238000012300 Sequence Analysis Methods 0.000 description 1
- 102100028904 Serine/threonine-protein kinase MARK2 Human genes 0.000 description 1
- 241000700584 Simplexvirus Species 0.000 description 1
- 108010014480 T-box transcription factor 5 Proteins 0.000 description 1
- 102100024755 T-box transcription factor TBX5 Human genes 0.000 description 1
- 101710137500 T7 RNA polymerase Proteins 0.000 description 1
- 108091046869 Telomeric non-coding RNA Proteins 0.000 description 1
- 239000004098 Tetracycline Substances 0.000 description 1
- 108010022394 Threonine synthase Proteins 0.000 description 1
- 102000006601 Thymidine Kinase Human genes 0.000 description 1
- 108020004440 Thymidine kinase Proteins 0.000 description 1
- 241001414989 Thysanoptera Species 0.000 description 1
- 102000003978 Tissue Plasminogen Activator Human genes 0.000 description 1
- 108090000373 Tissue Plasminogen Activator Proteins 0.000 description 1
- 108010048999 Transcription Factor 3 Proteins 0.000 description 1
- 102100038313 Transcription factor E2-alpha Human genes 0.000 description 1
- 102100021380 Transcription factor GATA-4 Human genes 0.000 description 1
- 102000005937 Tropomyosin Human genes 0.000 description 1
- 108010030743 Tropomyosin Proteins 0.000 description 1
- 102000004243 Tubulin Human genes 0.000 description 1
- 108090000704 Tubulin Proteins 0.000 description 1
- 108091023045 Untranslated Region Proteins 0.000 description 1
- DRTQHJPVMGBUCF-XVFCMESISA-N Uridine Chemical group O[C@@H]1[C@H](O)[C@@H](CO)O[C@H]1N1C(=O)NC(=O)C=C1 DRTQHJPVMGBUCF-XVFCMESISA-N 0.000 description 1
- 102000003990 Urokinase-type plasminogen activator Human genes 0.000 description 1
- 108090000435 Urokinase-type plasminogen activator Proteins 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 101100460507 Xenopus laevis nkx-2.5 gene Proteins 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 230000005856 abnormality Effects 0.000 description 1
- 125000000218 acetic acid group Chemical group C(C)(=O)* 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- 239000008186 active pharmaceutical agent Substances 0.000 description 1
- 229960000643 adenine Drugs 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 239000000556 agonist Substances 0.000 description 1
- 125000000217 alkyl group Chemical group 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 150000001412 amines Chemical class 0.000 description 1
- 238000003277 amino acid sequence analysis Methods 0.000 description 1
- 125000003277 amino group Chemical group 0.000 description 1
- 239000004599 antimicrobial Substances 0.000 description 1
- 239000002825 antisense oligoribonucleotide Substances 0.000 description 1
- 238000003782 apoptosis assay Methods 0.000 description 1
- 230000010455 autoregulation Effects 0.000 description 1
- 201000006069 autosomal dominant nonsyndromic deafness 10 Diseases 0.000 description 1
- 208000035499 autosomal dominant nonsyndromic hearing loss 10 Diseases 0.000 description 1
- 125000000852 azido group Chemical group *N=[N+]=[N-] 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 208000036815 beta tubulin Diseases 0.000 description 1
- HMFHBZSHGGEWLO-TXICZTDVSA-N beta-D-ribose Chemical group OC[C@H]1O[C@@H](O)[C@H](O)[C@@H]1O HMFHBZSHGGEWLO-TXICZTDVSA-N 0.000 description 1
- 108010005774 beta-Galactosidase Proteins 0.000 description 1
- 102000006635 beta-lactamase Human genes 0.000 description 1
- 229910002056 binary alloy Inorganic materials 0.000 description 1
- 108091008324 binding proteins Proteins 0.000 description 1
- 238000012742 biochemical analysis Methods 0.000 description 1
- 238000002306 biochemical method Methods 0.000 description 1
- 230000008033 biological extinction Effects 0.000 description 1
- 239000012620 biological material Substances 0.000 description 1
- 230000008512 biological response Effects 0.000 description 1
- 239000012472 biological sample Substances 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 125000004057 biotinyl group Chemical group [H]N1C(=O)N([H])[C@]2([H])[C@@]([H])(SC([H])([H])[C@]12[H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C(*)=O 0.000 description 1
- 210000002459 blastocyst Anatomy 0.000 description 1
- 229940098773 bovine serum albumin Drugs 0.000 description 1
- 210000004556 brain Anatomy 0.000 description 1
- 230000036952 cancer formation Effects 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 231100000504 carcinogenesis Toxicity 0.000 description 1
- 230000009084 cardiovascular function Effects 0.000 description 1
- 230000030833 cell death Effects 0.000 description 1
- 230000011712 cell development Effects 0.000 description 1
- 230000032823 cell division Effects 0.000 description 1
- 230000003915 cell function Effects 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 238000001516 cell proliferation assay Methods 0.000 description 1
- 108091092356 cellular DNA Proteins 0.000 description 1
- 108091092328 cellular RNA Proteins 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- 238000004182 chemical digestion Methods 0.000 description 1
- 230000000723 chemosensory effect Effects 0.000 description 1
- 210000003483 chromatin Anatomy 0.000 description 1
- 239000003593 chromogenic compound Substances 0.000 description 1
- 239000011436 cob Substances 0.000 description 1
- 229920001436 collagen Polymers 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 239000003184 complementary RNA Substances 0.000 description 1
- 239000000039 congener Substances 0.000 description 1
- 239000000356 contaminant Substances 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000012937 correction Methods 0.000 description 1
- 101150051397 csn3 gene Proteins 0.000 description 1
- 230000001086 cytosolic effect Effects 0.000 description 1
- 231100000895 deafness Toxicity 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 230000006735 deficit Effects 0.000 description 1
- 230000001934 delay Effects 0.000 description 1
- 238000009795 derivation Methods 0.000 description 1
- 201000001981 dermatomyositis Diseases 0.000 description 1
- 239000003599 detergent Substances 0.000 description 1
- QMQBBUPJKANITL-MYXGOWFTSA-N dextropropoxyphene hydrochloride Chemical compound [H+].[Cl-].C([C@](OC(=O)CC)([C@H](C)CN(C)C)C=1C=CC=CC=1)C1=CC=CC=C1 QMQBBUPJKANITL-MYXGOWFTSA-N 0.000 description 1
- 102000004419 dihydrofolate reductase Human genes 0.000 description 1
- 108020001096 dihydrofolate reductase Proteins 0.000 description 1
- PGUYAANYCROBRT-UHFFFAOYSA-N dihydroxy-selanyl-selanylidene-lambda5-phosphane Chemical compound OP(O)([SeH])=[Se] PGUYAANYCROBRT-UHFFFAOYSA-N 0.000 description 1
- 239000000539 dimer Substances 0.000 description 1
- 238000006471 dimerization reaction Methods 0.000 description 1
- NAGJZTKCGNOGPW-UHFFFAOYSA-K dioxido-sulfanylidene-sulfido-$l^{5}-phosphane Chemical compound [O-]P([O-])([S-])=S NAGJZTKCGNOGPW-UHFFFAOYSA-K 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 239000012636 effector Substances 0.000 description 1
- 210000002253 embryonic cardiomyocyte Anatomy 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000001317 epifluorescence microscopy Methods 0.000 description 1
- 150000002148 esters Chemical class 0.000 description 1
- 150000002170 ethers Chemical class 0.000 description 1
- ZMMJGEGLRURXTF-UHFFFAOYSA-N ethidium bromide Chemical compound [Br-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 ZMMJGEGLRURXTF-UHFFFAOYSA-N 0.000 description 1
- 229960005542 ethidium bromide Drugs 0.000 description 1
- 210000003527 eukaryotic cell Anatomy 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 102000013165 exonuclease Human genes 0.000 description 1
- 101150098472 eya4 gene Proteins 0.000 description 1
- 230000016221 eye-antennal disc development Effects 0.000 description 1
- 210000002458 fetal heart Anatomy 0.000 description 1
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 description 1
- 238000000799 fluorescence microscopy Methods 0.000 description 1
- 238000001943 fluorescence-activated cell sorting Methods 0.000 description 1
- 239000007850 fluorescent dye Substances 0.000 description 1
- 102000034287 fluorescent proteins Human genes 0.000 description 1
- 108091006047 fluorescent proteins Proteins 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 230000005714 functional activity Effects 0.000 description 1
- 238000001502 gel electrophoresis Methods 0.000 description 1
- 238000012224 gene deletion Methods 0.000 description 1
- 230000030279 gene silencing Effects 0.000 description 1
- 238000010363 gene targeting Methods 0.000 description 1
- 210000004602 germ cell Anatomy 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 230000002414 glycolytic effect Effects 0.000 description 1
- 230000036433 growing body Effects 0.000 description 1
- 239000003102 growth factor Substances 0.000 description 1
- 229940029575 guanosine Drugs 0.000 description 1
- 230000003394 haemopoietic effect Effects 0.000 description 1
- 229910052736 halogen Inorganic materials 0.000 description 1
- 150000002367 halogens Chemical class 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 208000016354 hearing loss disease Diseases 0.000 description 1
- 210000002837 heart atrium Anatomy 0.000 description 1
- 210000002064 heart cell Anatomy 0.000 description 1
- 230000004398 heart morphogenesis Effects 0.000 description 1
- 230000000004 hemodynamic effect Effects 0.000 description 1
- 230000011132 hemopoiesis Effects 0.000 description 1
- 125000000623 heterocyclic group Chemical group 0.000 description 1
- 102000055214 human SRF Human genes 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 206010020871 hypertrophic cardiomyopathy Diseases 0.000 description 1
- 230000001969 hypertrophic effect Effects 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 238000013388 immunohistochemistry analysis Methods 0.000 description 1
- 230000001771 impaired effect Effects 0.000 description 1
- 238000002513 implantation Methods 0.000 description 1
- 230000002779 inactivation Effects 0.000 description 1
- 239000000411 inducer Substances 0.000 description 1
- 238000001802 infusion Methods 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 208000021005 inheritance pattern Diseases 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 229940125396 insulin Drugs 0.000 description 1
- 230000000968 intestinal effect Effects 0.000 description 1
- 238000001361 intraarterial administration Methods 0.000 description 1
- 238000007918 intramuscular administration Methods 0.000 description 1
- 229940125425 inverse agonist Drugs 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 230000002427 irreversible effect Effects 0.000 description 1
- 238000012933 kinetic analysis Methods 0.000 description 1
- 238000011813 knockout mouse model Methods 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 231100000518 lethal Toxicity 0.000 description 1
- 230000001665 lethal effect Effects 0.000 description 1
- 239000003446 ligand Substances 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 230000003137 locomotive effect Effects 0.000 description 1
- 210000003141 lower extremity Anatomy 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 230000010311 mammalian development Effects 0.000 description 1
- 238000013507 mapping Methods 0.000 description 1
- 238000004949 mass spectrometry Methods 0.000 description 1
- 230000013011 mating Effects 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 description 1
- 108091044592 miR-1-1 stem-loop Proteins 0.000 description 1
- 108091063445 miR-13 stem-loop Proteins 0.000 description 1
- 108091049773 miR-14 stem-loop Proteins 0.000 description 1
- 108091028838 miR-2 stem-loop Proteins 0.000 description 1
- 108091071651 miR-208 stem-loop Proteins 0.000 description 1
- 108091084446 miR-208a stem-loop Proteins 0.000 description 1
- 108091062547 miR-208a-1 stem-loop Proteins 0.000 description 1
- 108091055375 miR-208a-2 stem-loop Proteins 0.000 description 1
- 108091046553 miR-26 stem-loop Proteins 0.000 description 1
- 108091023821 miR-26-1 stem-loop Proteins 0.000 description 1
- 108091045094 miR-26-2 stem-loop Proteins 0.000 description 1
- 108091007431 miR-29 Proteins 0.000 description 1
- 108091084679 miR-3 stem-loop Proteins 0.000 description 1
- 108091033354 miR-3-1 stem-loop Proteins 0.000 description 1
- 108091058771 miR-3-2 stem-loop Proteins 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 238000001000 micrograph Methods 0.000 description 1
- 238000007431 microscopic evaluation Methods 0.000 description 1
- 208000024191 minimally invasive lung adenocarcinoma Diseases 0.000 description 1
- 230000004898 mitochondrial function Effects 0.000 description 1
- 239000003068 molecular probe Substances 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 210000005087 mononuclear cell Anatomy 0.000 description 1
- 230000004220 muscle function Effects 0.000 description 1
- 230000002107 myocardial effect Effects 0.000 description 1
- 210000000653 nervous system Anatomy 0.000 description 1
- 208000015122 neurodegenerative disease Diseases 0.000 description 1
- 208000018360 neuromuscular disease Diseases 0.000 description 1
- 230000004031 neuronal differentiation Effects 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 238000010449 nuclear transplantation Methods 0.000 description 1
- 238000007899 nucleic acid hybridization Methods 0.000 description 1
- 239000002853 nucleic acid probe Substances 0.000 description 1
- 210000004940 nucleus Anatomy 0.000 description 1
- 229940046166 oligodeoxynucleotide Drugs 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 239000005022 packaging material Substances 0.000 description 1
- 239000004031 partial agonist Substances 0.000 description 1
- 230000036961 partial effect Effects 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- RGCLLPNLLBQHPF-HJWRWDBZSA-N phosphamidon Chemical compound CCN(CC)C(=O)C(\Cl)=C(/C)OP(=O)(OC)OC RGCLLPNLLBQHPF-HJWRWDBZSA-N 0.000 description 1
- KCRZDTROFIOPBP-UHFFFAOYSA-N phosphono 2,3-dihydroxypropanoate Chemical compound OCC(O)C(=O)OP(O)(O)=O KCRZDTROFIOPBP-UHFFFAOYSA-N 0.000 description 1
- PTMHPRAIXMAOOB-UHFFFAOYSA-L phosphoramidate Chemical compound NP([O-])([O-])=O PTMHPRAIXMAOOB-UHFFFAOYSA-L 0.000 description 1
- 108010031345 placental alkaline phosphatase Proteins 0.000 description 1
- 229920002647 polyamide Polymers 0.000 description 1
- 239000001267 polyvinylpyrrolidone Substances 0.000 description 1
- 235000013855 polyvinylpyrrolidone Nutrition 0.000 description 1
- 229920000036 polyvinylpyrrolidone Polymers 0.000 description 1
- 230000007542 postnatal development Effects 0.000 description 1
- 230000003334 potential effect Effects 0.000 description 1
- 244000144977 poultry Species 0.000 description 1
- 235000013594 poultry meat Nutrition 0.000 description 1
- 230000005522 programmed cell death Effects 0.000 description 1
- 210000001236 prokaryotic cell Anatomy 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 238000001243 protein synthesis Methods 0.000 description 1
- 230000004850 protein–protein interaction Effects 0.000 description 1
- 238000010188 recombinant method Methods 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000007261 regionalization Effects 0.000 description 1
- 230000014493 regulation of gene expression Effects 0.000 description 1
- 238000007634 remodeling Methods 0.000 description 1
- 230000010410 reperfusion Effects 0.000 description 1
- 230000003362 replicative effect Effects 0.000 description 1
- 108091008146 restriction endonucleases Proteins 0.000 description 1
- 230000001177 retroviral effect Effects 0.000 description 1
- 238000012552 review Methods 0.000 description 1
- 210000003705 ribosome Anatomy 0.000 description 1
- 235000019515 salmon Nutrition 0.000 description 1
- 210000000518 sarcolemma Anatomy 0.000 description 1
- JRPHGDYSKGJTKZ-UHFFFAOYSA-K selenophosphate Chemical compound [O-]P([O-])([O-])=[Se] JRPHGDYSKGJTKZ-UHFFFAOYSA-K 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 231100000879 sensorineural hearing loss Toxicity 0.000 description 1
- 208000023573 sensorineural hearing loss disease Diseases 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 230000004096 skeletal muscle tissue growth Effects 0.000 description 1
- 230000004877 small RNA loading onto RISC Effects 0.000 description 1
- 150000003384 small molecules Chemical class 0.000 description 1
- 210000002460 smooth muscle Anatomy 0.000 description 1
- 210000000329 smooth muscle myocyte Anatomy 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 230000033451 somitogenesis Effects 0.000 description 1
- 125000006850 spacer group Chemical group 0.000 description 1
- 238000002798 spectrophotometry method Methods 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 230000004936 stimulating effect Effects 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 238000007920 subcutaneous administration Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- 229910052717 sulfur Inorganic materials 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- 238000007910 systemic administration Methods 0.000 description 1
- 101150115978 tbx5 gene Proteins 0.000 description 1
- 108010057210 telomerase RNA Proteins 0.000 description 1
- 229960002180 tetracycline Drugs 0.000 description 1
- 229930101283 tetracycline Natural products 0.000 description 1
- 235000019364 tetracycline Nutrition 0.000 description 1
- 150000003522 tetracyclines Chemical class 0.000 description 1
- 230000004797 therapeutic response Effects 0.000 description 1
- RYYWUUFWQRZTIU-UHFFFAOYSA-K thiophosphate Chemical compound [O-]P([O-])([O-])=S RYYWUUFWQRZTIU-UHFFFAOYSA-K 0.000 description 1
- 229940113082 thymine Drugs 0.000 description 1
- 229960000187 tissue plasminogen activator Drugs 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 108091006106 transcriptional activators Proteins 0.000 description 1
- 230000006694 transcriptional co-activation Effects 0.000 description 1
- 230000037317 transdermal delivery Effects 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 238000003146 transient transfection Methods 0.000 description 1
- 230000007704 transition Effects 0.000 description 1
- 230000017105 transposition Effects 0.000 description 1
- 208000014903 transposition of the great arteries Diseases 0.000 description 1
- 241000701447 unidentified baculovirus Species 0.000 description 1
- 238000012762 unpaired Student’s t-test Methods 0.000 description 1
- 229960005356 urokinase Drugs 0.000 description 1
- 108700026220 vif Genes Proteins 0.000 description 1
- 239000013603 viral vector Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/7088—Compounds having three or more nucleosides or nucleotides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
- A61P17/02—Drugs for dermatological disorders for treating wounds, ulcers, burns, scars, keloids, or the like
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P21/00—Drugs for disorders of the muscular or neuromuscular system
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P21/00—Drugs for disorders of the muscular or neuromuscular system
- A61P21/04—Drugs for disorders of the muscular or neuromuscular system for myasthenia gravis
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P3/00—Drugs for disorders of the metabolism
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P5/00—Drugs for disorders of the endocrine system
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P9/00—Drugs for disorders of the cardiovascular system
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/11—Antisense
- C12N2310/113—Antisense targeting other non-coding nucleic acids, e.g. antagomirs
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/14—Type of nucleic acid interfering nucleic acids [NA]
- C12N2310/141—MicroRNAs, miRNAs
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/30—Chemical structure
- C12N2310/32—Chemical structure of the sugar
- C12N2310/321—2'-O-R Modification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/30—Chemical structure
- C12N2310/35—Nature of the modification
- C12N2310/351—Conjugate
- C12N2310/3517—Marker; Tag
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2330/00—Production
- C12N2330/10—Production naturally occurring
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Public Health (AREA)
- Medicinal Chemistry (AREA)
- Veterinary Medicine (AREA)
- Pharmacology & Pharmacy (AREA)
- Animal Behavior & Ethology (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Epidemiology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Neurology (AREA)
- Physical Education & Sports Medicine (AREA)
- Diabetes (AREA)
- Orthopedic Medicine & Surgery (AREA)
- Hematology (AREA)
- Obesity (AREA)
- Dermatology (AREA)
- Heart & Thoracic Surgery (AREA)
- Endocrinology (AREA)
- Cardiology (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US74954405P | 2005-12-12 | 2005-12-12 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| PT1969125E true PT1969125E (pt) | 2012-08-16 |
Family
ID=38163464
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PT06845222T PT1969125E (pt) | 2005-12-12 | 2006-12-12 | Microarns que regulam a proliferação e diferenciação de células musculares |
Country Status (14)
| Country | Link |
|---|---|
| US (3) | US8431542B2 (enExample) |
| EP (3) | EP2671949A1 (enExample) |
| JP (3) | JP2009519339A (enExample) |
| KR (2) | KR101485071B1 (enExample) |
| CN (2) | CN101448942B (enExample) |
| AU (1) | AU2006326517B2 (enExample) |
| BR (1) | BRPI0619755A2 (enExample) |
| CA (2) | CA2634046A1 (enExample) |
| DK (1) | DK1969125T3 (enExample) |
| ES (1) | ES2387250T3 (enExample) |
| NZ (3) | NZ569738A (enExample) |
| PL (1) | PL1969125T3 (enExample) |
| PT (1) | PT1969125E (enExample) |
| WO (1) | WO2007070483A2 (enExample) |
Families Citing this family (64)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2006107826A2 (en) | 2005-04-04 | 2006-10-12 | The Board Of Regents Of The University Of Texas System | Micro-rna's that regulate muscle cells |
| CN101448942B (zh) | 2005-12-12 | 2014-04-23 | 北卡罗来纳大学查珀尔希尔分校 | 调节肌细胞增殖和分化的microrna |
| EP2434017A3 (en) | 2006-08-01 | 2012-09-05 | Board of Regents of the University of Texas System | Identification of a micro-RNA that activates expression of beta-myosin heavy chain |
| GB2440782A (en) * | 2006-08-04 | 2008-02-13 | Cesare Peschle | Uses and compositions comprising miRNAs |
| HUE037173T2 (hu) | 2006-08-08 | 2018-08-28 | Univ Bonn Rheinische Friedrich Wilhelms | 5'-Foszfát-oligonukleotidok szerkezete és alkalmazása |
| CA2728575A1 (en) | 2007-06-29 | 2009-01-08 | Boston Biomedical, Inc. | Enabling the use of long dsrna for gene targeting in mammalian and other selected animal cells |
| WO2009018492A2 (en) * | 2007-07-31 | 2009-02-05 | The Board Of Regents Of The University Of Texas System | Micro-rnas that control myosin expression and myofiber identity |
| ES2621161T3 (es) * | 2007-07-31 | 2017-07-03 | The Board Of Regents Of The University Of Texas System | Familia de micro-ARN que modula la fibrosis y usos de la misma |
| CA2716343A1 (en) * | 2008-02-21 | 2009-08-27 | The Board Of Regents Of The University Of Texas System | Micro-rnas that modulate smooth muscle proliferation and differentiation and uses thereof |
| CA2718520C (en) * | 2008-03-17 | 2020-01-07 | The Board Of Regents Of The University Of Texas System | Identification of micro-rnas involved in neuromuscular synapse maintenance and regeneration |
| EP2297323A1 (en) | 2008-05-21 | 2011-03-23 | Hartmann, Gunther | 5' triphosphate oligonucleotide with blunt end and uses thereof |
| AU2009257663B2 (en) * | 2008-06-09 | 2014-06-26 | New York Medical College | Compositions comprising cardiac stem cells overexpressing specific microRNA and methods of their use in repairing damaged myocardium |
| GB0811715D0 (en) * | 2008-06-26 | 2008-07-30 | Angeletti P Ist Richerche Bio | Therapeutic agents |
| MX2011008190A (es) | 2009-02-04 | 2011-10-06 | Univ Texas | Direccionamiento dual de mir-208 y mir-499 en el tratamiento de trastornos cardiacos. |
| DK2427472T3 (en) | 2009-05-05 | 2016-09-12 | Miragen Therapeutics | Lipophilic polynukleotidkonjugater |
| EP2435570A1 (en) * | 2009-05-25 | 2012-04-04 | Carola Ponzetto | Differentiation therapy for sarcomas |
| US20130034850A1 (en) * | 2010-02-26 | 2013-02-07 | Natinal Center of Neurology and Psychiatry | Marker for detecting myogenic disease and detection method using the same |
| KR20130069587A (ko) * | 2010-04-08 | 2013-06-26 | 교토후고리츠다이가쿠호진 | 체액 유래 검체를 사용한 횡문근육종의 검출 방법 |
| US9987309B2 (en) * | 2010-07-08 | 2018-06-05 | Duke University | Direct reprogramming of cells to cardiac myocyte fate |
| KR101235256B1 (ko) * | 2010-09-13 | 2013-02-21 | 서울대학교산학협력단 | ｍｉＲＮＡ를 타겟으로 한 신경퇴행성 질환 치료 |
| AU2011323085B2 (en) | 2010-11-05 | 2014-10-30 | Miragen Therapeutics | Base modified oligonucleotides |
| EP2638158A4 (en) * | 2010-11-11 | 2015-07-15 | Univ Miami | COMPOSITIONS, CELLS, KITS AND METHODS FOR AUTOLOGOUS STEM CELL THERAPY |
| US8642751B2 (en) | 2010-12-15 | 2014-02-04 | Miragen Therapeutics | MicroRNA inhibitors comprising locked nucleotides |
| EP2508530A1 (en) | 2011-03-28 | 2012-10-10 | Rheinische Friedrich-Wilhelms-Universität Bonn | Purification of triphosphorylated oligonucleotides using capture tags |
| CN103814131A (zh) * | 2011-05-09 | 2014-05-21 | 格拉斯哥大学理事会 | 在肺动脉高血压的治疗中调控microrna的方法 |
| US20140221464A1 (en) * | 2011-07-01 | 2014-08-07 | Board Of Regents, The University Of Texas System | Compositions and Methods for Treating Skeletal Myopathy |
| EP2731422B1 (en) * | 2011-07-12 | 2017-08-09 | Mart Saarma | miRNA antagonists for use in treating neurodegenerative disorders by increasing expression of GDNF |
| US9018188B2 (en) | 2011-09-13 | 2015-04-28 | Ottawa Hospital Research Institute | MicroRNA inhibitors |
| US9428749B2 (en) * | 2011-10-06 | 2016-08-30 | The Board Of Regents, The University Of Texas System | Control of whole body energy homeostasis by microRNA regulation |
| JP6208228B2 (ja) | 2012-06-21 | 2017-10-04 | ミラゲン セラピューティクス， インコーポレイテッド | ロックド核酸モチーフを含むオリゴヌクレオチドベースの阻害剤 |
| EP2712870A1 (en) | 2012-09-27 | 2014-04-02 | Rheinische Friedrich-Wilhelms-Universität Bonn | Novel RIG-I ligands and methods for producing them |
| US9752143B2 (en) | 2013-03-15 | 2017-09-05 | MiRagen Therapeutics, Inc. | Locked nucleic acid inhibitor of miR-145 and uses thereof |
| EP2970968B1 (en) | 2013-03-15 | 2018-01-10 | Miragen Therapeutics, Inc. | Bridged bicyclic nucleosides |
| WO2014201254A1 (en) * | 2013-06-12 | 2014-12-18 | University Of Washington Through Its Center For Commercialization | Methods for maturing cardiomyocytes and uses thereof |
| CN103710387B (zh) * | 2013-12-25 | 2015-10-14 | 天津农学院 | 一种促进牛骨骼肌卫星细胞体外增殖的方法 |
| JP6566536B2 (ja) * | 2014-06-16 | 2019-08-28 | 国立大学法人山口大学 | フォワードプライマー |
| CN104498497A (zh) * | 2014-12-10 | 2015-04-08 | 山东农业大学 | 一种与奶山羊乳腺上皮细胞凋亡相关的miRNA及其在奶山羊育种中的应用 |
| US10620109B2 (en) | 2015-01-14 | 2020-04-14 | Kyoto University | Method for selecting skeletal muscle progenitor cell |
| EP3247716A4 (en) | 2015-01-20 | 2018-10-17 | Miragen Therapeutics, Inc. | Mir-92 inhibitors and uses thereof |
| CN104877960B (zh) * | 2015-06-01 | 2017-09-19 | 东北农业大学 | miR‑2400显著促进牛骨骼肌卫星细胞增殖的生物功能 |
| CN117757794A (zh) | 2016-03-07 | 2024-03-26 | 美国政府(由卫生和人类服务部的部长所代表) | 微小rna及其使用方法 |
| CN105713972A (zh) * | 2016-03-16 | 2016-06-29 | 上海中医药大学 | miRNA在制备药物性心脏病生物标志物中的用途 |
| CN106399473B (zh) * | 2016-08-23 | 2019-12-06 | 南京大学 | 检测和评价力量训练效果的miRNA标志物或其组合及其应用 |
| EP3527212A4 (en) * | 2016-10-13 | 2020-05-06 | National Center of Neurology and Psychiatry | Myogenesis inducing agent |
| WO2018080658A1 (en) * | 2016-10-27 | 2018-05-03 | Aalborg University | Therapeutic targeting of a microrna to treat duchenne muscular dystrophy |
| CN108324946B (zh) * | 2017-01-20 | 2022-02-18 | 上海市东方医院 | miRNA708、和/或301簇的微小RNA在改善心脏功能方面的应用 |
| CN107096030A (zh) * | 2017-03-20 | 2017-08-29 | 天津农学院 | 一种利用miR‑143抑制剂延缓小鼠失神经后肌肉萎缩的方法 |
| WO2018187523A2 (en) * | 2017-04-05 | 2018-10-11 | University Of Cincinnati | Promotion of cardiomyocyte proliferation and regenerative treatment of the heart by inhibition of microrna-128 |
| WO2019035690A1 (ko) * | 2017-08-18 | 2019-02-21 | 서울대학교병원 | 근육 질환 및 신경근육 질환 예방, 치료 또는 진단을 위한 miR-18b의 용도 |
| KR102177130B1 (ko) | 2017-08-18 | 2020-11-10 | (주)큐라미스 | 근육 질환 및 신경근육 질환 예방, 치료 또는 진단을 위한 miR-18b의 용도 |
| WO2019113432A1 (en) * | 2017-12-08 | 2019-06-13 | University Of Washington | Methods and compositions for detecting and promoting cardiolipin remodeling and cardiomyocyte maturation and related methods of treating mitochondrial dysfunction |
| JP7318166B2 (ja) * | 2018-03-14 | 2023-08-01 | ベス イスラエル デアコネス メディカル センター | マイクロｒｎａ２２の阻害剤 |
| CN108690846A (zh) * | 2018-04-17 | 2018-10-23 | 上海大学 | 抑制MiR-29b基因表达的应用 |
| CN108823209B (zh) * | 2018-07-18 | 2021-04-09 | 华中农业大学 | 骨骼肌特异性启动子及应用 |
| AU2019333315B2 (en) | 2018-08-30 | 2022-09-29 | Tenaya Therapeutics, Inc. | Cardiac cell reprogramming with myocardin and ASCL1 |
| CN111041052B (zh) * | 2019-12-30 | 2021-05-28 | 嘉兴学院 | mir-206在抑制猪脂肪细胞内的甘油三酯生成以及瘦肉猪育种中的应用 |
| CA3173567A1 (en) * | 2020-03-02 | 2021-09-10 | Tenaya Therapeutics, Inc. | Gene vector control by cardiomyocyte-expressed micrornas |
| EP4127175A4 (en) | 2020-04-02 | 2025-01-22 | Mirecule, Inc. | Targeted inhibition using engineered oligonucleotides |
| CN112266912A (zh) * | 2020-10-22 | 2021-01-26 | 上海大学 | 靶向miR-29b的gRNA、AAV8-CRISPR/Cas9系统及其应用 |
| WO2023034966A1 (en) * | 2021-09-03 | 2023-03-09 | The University Of North Carolina At Chapel Hill | Compositions and methods of using the same for treating disorders associated with thymosin βeta 4 |
| CN114480672B (zh) * | 2022-02-22 | 2023-08-15 | 青岛农业大学 | 一种通过miR-145筛选高产肉率碱地黑牛的方法 |
| CN114748497B (zh) * | 2022-04-27 | 2024-10-18 | 广州市妇女儿童医疗中心 | 一种调控子宫肌收缩的miRNA分子miR-206及其应用 |
| CN115181722B (zh) * | 2022-08-30 | 2024-06-11 | 江苏农牧科技职业学院 | 一种鹅骨骼肌卫星细胞的体外分离和培养方法 |
| CN117511884B (zh) * | 2023-12-06 | 2024-06-11 | 中国农业科学院兰州畜牧与兽药研究所 | Rtl1在抑制牦牛成肌细胞分化中的应用 |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7803782B2 (en) * | 2003-05-28 | 2010-09-28 | Roche Madison Inc. | Intravenous delivery of polynucleotides to cells in mammalian limb |
| US20050124566A1 (en) | 2001-05-18 | 2005-06-09 | Sirna Therapeutics, Inc. | RNA interference mediated inhibition of myostatin gene expression using short interfering nucleic acid (siNA) |
| EP2390328A1 (en) * | 2001-09-28 | 2011-11-30 | Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. | MicroRNA molecules |
| US7781415B2 (en) * | 2003-02-07 | 2010-08-24 | Roche Madison Inc. | Process for delivering sirna to cardiac muscle tissue |
| KR20050115231A (ko) * | 2003-02-10 | 2005-12-07 | 내셔날 인스티튜트 오브 어드밴스드 인더스트리얼 사이언스 앤드 테크놀로지 | 포유동물 세포의 조절 |
| SE521643C2 (sv) * | 2003-02-26 | 2003-11-18 | Scania Cv Publ | Kraftöverföring med från en huvudväxel skild rangeväxel |
| EP2314688B1 (en) * | 2004-11-12 | 2014-07-16 | Asuragen, Inc. | Methods and compositions involving miRNA and miRNA inhibitor molecules |
| WO2006107826A2 (en) | 2005-04-04 | 2006-10-12 | The Board Of Regents Of The University Of Texas System | Micro-rna's that regulate muscle cells |
| WO2006113540A2 (en) | 2005-04-14 | 2006-10-26 | Duke University | Use of ntrosylated hemoglobin |
| CN101448942B (zh) | 2005-12-12 | 2014-04-23 | 北卡罗来纳大学查珀尔希尔分校 | 调节肌细胞增殖和分化的microrna |
-
2006
- 2006-12-12 CN CN200680052681.XA patent/CN101448942B/zh not_active Expired - Fee Related
- 2006-12-12 NZ NZ569738A patent/NZ569738A/en not_active IP Right Cessation
- 2006-12-12 EP EP13177425.9A patent/EP2671949A1/en not_active Withdrawn
- 2006-12-12 CA CA002634046A patent/CA2634046A1/en not_active Abandoned
- 2006-12-12 KR KR1020087017004A patent/KR101485071B1/ko not_active Expired - Fee Related
- 2006-12-12 WO PCT/US2006/047255 patent/WO2007070483A2/en not_active Ceased
- 2006-12-12 EP EP06845222A patent/EP1969125B1/en active Active
- 2006-12-12 BR BRPI0619755-8A patent/BRPI0619755A2/pt not_active IP Right Cessation
- 2006-12-12 CN CN201410112733.4A patent/CN104027818A/zh active Pending
- 2006-12-12 NZ NZ593688A patent/NZ593688A/xx not_active IP Right Cessation
- 2006-12-12 JP JP2008545718A patent/JP2009519339A/ja active Pending
- 2006-12-12 CA CA2903764A patent/CA2903764A1/en not_active Abandoned
- 2006-12-12 DK DK06845222.6T patent/DK1969125T3/da active
- 2006-12-12 PT PT06845222T patent/PT1969125E/pt unknown
- 2006-12-12 EP EP12166173A patent/EP2497827A1/en not_active Withdrawn
- 2006-12-12 AU AU2006326517A patent/AU2006326517B2/en not_active Ceased
- 2006-12-12 NZ NZ602571A patent/NZ602571A/en not_active IP Right Cessation
- 2006-12-12 PL PL06845222T patent/PL1969125T3/pl unknown
- 2006-12-12 US US12/086,109 patent/US8431542B2/en not_active Expired - Fee Related
- 2006-12-12 ES ES06845222T patent/ES2387250T3/es active Active
- 2006-12-12 KR KR1020147028814A patent/KR20140130562A/ko not_active Ceased
-
2013
- 2013-02-15 JP JP2013027633A patent/JP5796027B2/ja not_active Expired - Fee Related
- 2013-03-08 US US13/791,736 patent/US20130225658A1/en not_active Abandoned
- 2013-03-08 US US13/791,309 patent/US9072764B2/en not_active Expired - Fee Related
- 2013-12-09 JP JP2013253829A patent/JP5740460B2/ja not_active Expired - Fee Related
Also Published As
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US8431542B2 (en) | Micrornas that regulate muscle cell proliferation and differentiation | |
| US10612026B2 (en) | Pharmaceutical composition for treating cancer comprising microrna as active ingredient | |
| CA2827533A1 (en) | Enhanced biodistribution of oligomers | |
| AU2013202293B2 (en) | MicroRNAs that regulate muscle cell proliferation and differentiation | |
| US20100305195A1 (en) | microrna mediator of cardiomyopathy and heart failure | |
| HK1192280A (en) | Micro-rna mir-145 that regulate muscle cell proliferation and differentiation | |
| MX2008007552A (es) | Microacidos ribonucleicos que regulan la proliferacion y diferenciacion de celulas musculares | |
| Ge | Regulation of skeletal myogenesis by the mTOR signaling network |