NO329977B1 - Fremgangsmate for pavisning av minst ett nukleinsyremal og sett for pavisning av minst ett nukleinsyremal i en prove - Google Patents
Fremgangsmate for pavisning av minst ett nukleinsyremal og sett for pavisning av minst ett nukleinsyremal i en prove Download PDFInfo
- Publication number
- NO329977B1 NO329977B1 NO20003115A NO20003115A NO329977B1 NO 329977 B1 NO329977 B1 NO 329977B1 NO 20003115 A NO20003115 A NO 20003115A NO 20003115 A NO20003115 A NO 20003115A NO 329977 B1 NO329977 B1 NO 329977B1
- Authority
- NO
- Norway
- Prior art keywords
- target
- nucleic acid
- specific
- anchors
- approx
- Prior art date
Links
- 238000000034 method Methods 0.000 title claims abstract description 127
- 150000007523 nucleic acids Chemical class 0.000 title claims description 117
- 102000039446 nucleic acids Human genes 0.000 title claims description 114
- 108020004707 nucleic acids Proteins 0.000 title claims description 114
- 239000000523 sample Substances 0.000 claims abstract description 262
- 230000001588 bifunctional effect Effects 0.000 claims abstract description 26
- 230000000694 effects Effects 0.000 claims abstract description 14
- 108091034117 Oligonucleotide Proteins 0.000 claims description 199
- 239000012634 fragment Substances 0.000 claims description 130
- 230000004224 protection Effects 0.000 claims description 126
- 238000009396 hybridization Methods 0.000 claims description 91
- 101710163270 Nuclease Proteins 0.000 claims description 90
- 108091032973 (ribonucleotides)n+m Proteins 0.000 claims description 84
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 claims description 66
- 238000001514 detection method Methods 0.000 claims description 60
- 230000014509 gene expression Effects 0.000 claims description 35
- 239000003795 chemical substances by application Substances 0.000 claims description 33
- 230000003993 interaction Effects 0.000 claims description 26
- 239000002773 nucleotide Substances 0.000 claims description 18
- 125000003729 nucleotide group Chemical group 0.000 claims description 18
- 201000010099 disease Diseases 0.000 claims description 15
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 15
- 239000000463 material Substances 0.000 claims description 12
- 239000003814 drug Substances 0.000 claims description 9
- 238000000338 in vitro Methods 0.000 claims description 6
- 230000003321 amplification Effects 0.000 claims description 4
- 238000001727 in vivo Methods 0.000 claims description 4
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 4
- 238000012408 PCR amplification Methods 0.000 claims 1
- 230000008672 reprogramming Effects 0.000 claims 1
- 230000001225 therapeutic effect Effects 0.000 claims 1
- 238000003556 assay Methods 0.000 abstract description 76
- 238000012360 testing method Methods 0.000 abstract description 68
- 239000000126 substance Substances 0.000 abstract description 45
- 238000003491 array Methods 0.000 abstract description 21
- 239000000203 mixture Substances 0.000 abstract description 19
- 238000007373 indentation Methods 0.000 abstract 1
- 125000005647 linker group Chemical group 0.000 description 143
- 108020004999 messenger RNA Proteins 0.000 description 82
- 108091060211 Expressed sequence tag Proteins 0.000 description 80
- 238000004458 analytical method Methods 0.000 description 62
- 108090000623 proteins and genes Proteins 0.000 description 58
- 230000000295 complement effect Effects 0.000 description 55
- 230000027455 binding Effects 0.000 description 54
- 238000009739 binding Methods 0.000 description 54
- 108020005187 Oligonucleotide Probes Proteins 0.000 description 44
- 239000002751 oligonucleotide probe Substances 0.000 description 44
- 108090000765 processed proteins & peptides Proteins 0.000 description 39
- 210000004027 cell Anatomy 0.000 description 38
- 108020004414 DNA Proteins 0.000 description 28
- 108091033319 polynucleotide Proteins 0.000 description 28
- 102000040430 polynucleotide Human genes 0.000 description 28
- 239000002157 polynucleotide Substances 0.000 description 26
- 230000000875 corresponding effect Effects 0.000 description 25
- 239000000758 substrate Substances 0.000 description 24
- 102000004190 Enzymes Human genes 0.000 description 23
- 108090000790 Enzymes Proteins 0.000 description 23
- 238000006243 chemical reaction Methods 0.000 description 23
- 150000001875 compounds Chemical class 0.000 description 23
- 239000000047 product Substances 0.000 description 23
- 102000004196 processed proteins & peptides Human genes 0.000 description 22
- 108091000080 Phosphotransferase Proteins 0.000 description 20
- 239000002299 complementary DNA Substances 0.000 description 20
- 102000020233 phosphotransferase Human genes 0.000 description 20
- 239000000872 buffer Substances 0.000 description 17
- 239000004033 plastic Substances 0.000 description 17
- 229920003023 plastic Polymers 0.000 description 17
- 102000004169 proteins and genes Human genes 0.000 description 17
- 238000011534 incubation Methods 0.000 description 16
- 108020003175 receptors Proteins 0.000 description 16
- 102000005962 receptors Human genes 0.000 description 16
- 102100031181 Glyceraldehyde-3-phosphate dehydrogenase Human genes 0.000 description 15
- 108020004445 glyceraldehyde-3-phosphate dehydrogenase Proteins 0.000 description 15
- 239000002585 base Substances 0.000 description 14
- 239000003446 ligand Substances 0.000 description 14
- 238000007792 addition Methods 0.000 description 13
- 239000003550 marker Substances 0.000 description 12
- 108010001336 Horseradish Peroxidase Proteins 0.000 description 11
- 238000003776 cleavage reaction Methods 0.000 description 11
- -1 rRNA Proteins 0.000 description 11
- 230000007017 scission Effects 0.000 description 11
- 230000035945 sensitivity Effects 0.000 description 11
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 10
- 102100034343 Integrase Human genes 0.000 description 10
- 108091005804 Peptidases Proteins 0.000 description 10
- 239000004365 Protease Substances 0.000 description 10
- 238000005516 engineering process Methods 0.000 description 10
- 239000000284 extract Substances 0.000 description 10
- 238000012216 screening Methods 0.000 description 10
- 238000005406 washing Methods 0.000 description 10
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 description 9
- 239000013615 primer Substances 0.000 description 9
- 238000011282 treatment Methods 0.000 description 9
- 102000035195 Peptidases Human genes 0.000 description 8
- 230000008901 benefit Effects 0.000 description 8
- 230000007423 decrease Effects 0.000 description 8
- 238000010790 dilution Methods 0.000 description 8
- 239000012895 dilution Substances 0.000 description 8
- 210000001519 tissue Anatomy 0.000 description 8
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 7
- 108091093037 Peptide nucleic acid Proteins 0.000 description 7
- 239000011521 glass Substances 0.000 description 7
- 238000002372 labelling Methods 0.000 description 7
- 230000001681 protective effect Effects 0.000 description 7
- 108090001090 Lectins Proteins 0.000 description 6
- 102000004856 Lectins Human genes 0.000 description 6
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
- 230000000692 anti-sense effect Effects 0.000 description 6
- 229960002685 biotin Drugs 0.000 description 6
- 239000011616 biotin Substances 0.000 description 6
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 description 6
- 239000002523 lectin Substances 0.000 description 6
- 238000002360 preparation method Methods 0.000 description 6
- 238000011002 quantification Methods 0.000 description 6
- 238000013518 transcription Methods 0.000 description 6
- 230000035897 transcription Effects 0.000 description 6
- OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 description 5
- 239000004793 Polystyrene Substances 0.000 description 5
- 102000014400 SH2 domains Human genes 0.000 description 5
- 108050003452 SH2 domains Proteins 0.000 description 5
- 108010090804 Streptavidin Proteins 0.000 description 5
- 208000037065 Subacute sclerosing leukoencephalitis Diseases 0.000 description 5
- 206010042297 Subacute sclerosing panencephalitis Diseases 0.000 description 5
- 150000001413 amino acids Chemical class 0.000 description 5
- 239000000427 antigen Substances 0.000 description 5
- 108091007433 antigens Proteins 0.000 description 5
- 102000036639 antigens Human genes 0.000 description 5
- 235000020958 biotin Nutrition 0.000 description 5
- 239000002738 chelating agent Substances 0.000 description 5
- 239000003153 chemical reaction reagent Substances 0.000 description 5
- 238000013461 design Methods 0.000 description 5
- 229940079593 drug Drugs 0.000 description 5
- 229940000406 drug candidate Drugs 0.000 description 5
- 238000003384 imaging method Methods 0.000 description 5
- 210000004185 liver Anatomy 0.000 description 5
- PXHVJJICTQNCMI-UHFFFAOYSA-N nickel Substances [Ni] PXHVJJICTQNCMI-UHFFFAOYSA-N 0.000 description 5
- 239000002245 particle Substances 0.000 description 5
- DCWXELXMIBXGTH-UHFFFAOYSA-N phosphotyrosine Chemical compound OC(=O)C(N)CC1=CC=C(OP(O)(O)=O)C=C1 DCWXELXMIBXGTH-UHFFFAOYSA-N 0.000 description 5
- 230000035790 physiological processes and functions Effects 0.000 description 5
- 229920002223 polystyrene Polymers 0.000 description 5
- 230000008569 process Effects 0.000 description 5
- 239000011541 reaction mixture Substances 0.000 description 5
- OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 5
- 230000004568 DNA-binding Effects 0.000 description 4
- 108700039887 Essential Genes Proteins 0.000 description 4
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 4
- 229940124639 Selective inhibitor Drugs 0.000 description 4
- 230000015572 biosynthetic process Effects 0.000 description 4
- 210000000170 cell membrane Anatomy 0.000 description 4
- 238000007796 conventional method Methods 0.000 description 4
- 238000007876 drug discovery Methods 0.000 description 4
- 239000012530 fluid Substances 0.000 description 4
- 238000013507 mapping Methods 0.000 description 4
- 238000004949 mass spectrometry Methods 0.000 description 4
- 238000005259 measurement Methods 0.000 description 4
- 229910052751 metal Inorganic materials 0.000 description 4
- 239000002184 metal Substances 0.000 description 4
- 229920000642 polymer Polymers 0.000 description 4
- 229920001296 polysiloxane Polymers 0.000 description 4
- 230000002829 reductive effect Effects 0.000 description 4
- 150000003839 salts Chemical class 0.000 description 4
- 102000053602 DNA Human genes 0.000 description 3
- 101001050288 Homo sapiens Transcription factor Jun Proteins 0.000 description 3
- 101710151803 Mitochondrial intermediate peptidase 2 Proteins 0.000 description 3
- 101000819572 Mus musculus Glyceraldehyde-3-phosphate dehydrogenase Proteins 0.000 description 3
- 206010028980 Neoplasm Diseases 0.000 description 3
- 108091028043 Nucleic acid sequence Proteins 0.000 description 3
- 108090000412 Protein-Tyrosine Kinases Proteins 0.000 description 3
- 102000004022 Protein-Tyrosine Kinases Human genes 0.000 description 3
- 239000013614 RNA sample Substances 0.000 description 3
- 108010083644 Ribonucleases Proteins 0.000 description 3
- 102000006382 Ribonucleases Human genes 0.000 description 3
- 102100023132 Transcription factor Jun Human genes 0.000 description 3
- 108020000999 Viral RNA Proteins 0.000 description 3
- 241000700605 Viruses Species 0.000 description 3
- 239000000556 agonist Substances 0.000 description 3
- 125000003277 amino group Chemical group 0.000 description 3
- 239000005557 antagonist Substances 0.000 description 3
- 239000011230 binding agent Substances 0.000 description 3
- 238000007385 chemical modification Methods 0.000 description 3
- 230000002860 competitive effect Effects 0.000 description 3
- 238000000605 extraction Methods 0.000 description 3
- 238000002073 fluorescence micrograph Methods 0.000 description 3
- 238000000799 fluorescence microscopy Methods 0.000 description 3
- 238000013467 fragmentation Methods 0.000 description 3
- 238000006062 fragmentation reaction Methods 0.000 description 3
- 239000004009 herbicide Substances 0.000 description 3
- 230000002452 interceptive effect Effects 0.000 description 3
- 150000002500 ions Chemical group 0.000 description 3
- 230000000670 limiting effect Effects 0.000 description 3
- 150000002632 lipids Chemical class 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 238000004020 luminiscence type Methods 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 230000004048 modification Effects 0.000 description 3
- 238000012986 modification Methods 0.000 description 3
- 230000035772 mutation Effects 0.000 description 3
- 239000013642 negative control Substances 0.000 description 3
- 229910052759 nickel Inorganic materials 0.000 description 3
- 244000052769 pathogen Species 0.000 description 3
- 238000003909 pattern recognition Methods 0.000 description 3
- 239000000575 pesticide Substances 0.000 description 3
- 230000026731 phosphorylation Effects 0.000 description 3
- 238000006366 phosphorylation reaction Methods 0.000 description 3
- 239000013641 positive control Substances 0.000 description 3
- 238000000159 protein binding assay Methods 0.000 description 3
- 238000000926 separation method Methods 0.000 description 3
- 150000003384 small molecules Chemical class 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 230000009870 specific binding Effects 0.000 description 3
- 238000001228 spectrum Methods 0.000 description 3
- 238000003786 synthesis reaction Methods 0.000 description 3
- 230000002103 transcriptional effect Effects 0.000 description 3
- 238000012546 transfer Methods 0.000 description 3
- JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 description 2
- KXDHJXZQYSOELW-UHFFFAOYSA-M Carbamate Chemical compound NC([O-])=O KXDHJXZQYSOELW-UHFFFAOYSA-M 0.000 description 2
- 108091033380 Coding strand Proteins 0.000 description 2
- 102000004163 DNA-directed RNA polymerases Human genes 0.000 description 2
- 108090000626 DNA-directed RNA polymerases Proteins 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- 238000004252 FT/ICR mass spectrometry Methods 0.000 description 2
- ZHNUHDYFZUAESO-UHFFFAOYSA-N Formamide Chemical compound NC=O ZHNUHDYFZUAESO-UHFFFAOYSA-N 0.000 description 2
- 239000007995 HEPES buffer Substances 0.000 description 2
- 229930010555 Inosine Natural products 0.000 description 2
- UGQMRVRMYYASKQ-KQYNXXCUSA-N Inosine Chemical compound O[C@@H]1[C@H](O)[C@@H](CO)O[C@H]1N1C2=NC=NC(O)=C2N=C1 UGQMRVRMYYASKQ-KQYNXXCUSA-N 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 108010086093 Mung Bean Nuclease Proteins 0.000 description 2
- 108020004711 Nucleic Acid Probes Proteins 0.000 description 2
- 108010038807 Oligopeptides Proteins 0.000 description 2
- 102000015636 Oligopeptides Human genes 0.000 description 2
- 229910019142 PO4 Inorganic materials 0.000 description 2
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 2
- 108010001441 Phosphopeptides Proteins 0.000 description 2
- 241001136629 Pixus Species 0.000 description 2
- 229920001213 Polysorbate 20 Polymers 0.000 description 2
- 102000001253 Protein Kinase Human genes 0.000 description 2
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 2
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
- 108020004682 Single-Stranded DNA Proteins 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 239000007983 Tris buffer Substances 0.000 description 2
- 239000013504 Triton X-100 Substances 0.000 description 2
- 229920004890 Triton X-100 Polymers 0.000 description 2
- 108020005202 Viral DNA Proteins 0.000 description 2
- 230000033228 biological regulation Effects 0.000 description 2
- 230000000903 blocking effect Effects 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 230000023555 blood coagulation Effects 0.000 description 2
- 229910052791 calcium Inorganic materials 0.000 description 2
- 239000011575 calcium Substances 0.000 description 2
- 230000015556 catabolic process Effects 0.000 description 2
- 230000007541 cellular toxicity Effects 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 238000010276 construction Methods 0.000 description 2
- 230000002596 correlated effect Effects 0.000 description 2
- 230000008878 coupling Effects 0.000 description 2
- 238000010168 coupling process Methods 0.000 description 2
- 238000005859 coupling reaction Methods 0.000 description 2
- 238000006731 degradation reaction Methods 0.000 description 2
- 238000010511 deprotection reaction Methods 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 239000013024 dilution buffer Substances 0.000 description 2
- 230000002708 enhancing effect Effects 0.000 description 2
- 230000005284 excitation Effects 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 238000002875 fluorescence polarization Methods 0.000 description 2
- 239000007850 fluorescent dye Substances 0.000 description 2
- 238000010438 heat treatment Methods 0.000 description 2
- 230000007062 hydrolysis Effects 0.000 description 2
- 238000006460 hydrolysis reaction Methods 0.000 description 2
- 239000003112 inhibitor Substances 0.000 description 2
- 229910010272 inorganic material Inorganic materials 0.000 description 2
- 239000011147 inorganic material Substances 0.000 description 2
- 229960003786 inosine Drugs 0.000 description 2
- 229910052749 magnesium Inorganic materials 0.000 description 2
- 239000011777 magnesium Substances 0.000 description 2
- 238000002844 melting Methods 0.000 description 2
- 230000008018 melting Effects 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 150000002739 metals Chemical class 0.000 description 2
- YACKEPLHDIMKIO-UHFFFAOYSA-N methylphosphonic acid Chemical compound CP(O)(O)=O YACKEPLHDIMKIO-UHFFFAOYSA-N 0.000 description 2
- 238000003032 molecular docking Methods 0.000 description 2
- 238000007899 nucleic acid hybridization Methods 0.000 description 2
- 239000002853 nucleic acid probe Substances 0.000 description 2
- 239000011368 organic material Substances 0.000 description 2
- 230000036961 partial effect Effects 0.000 description 2
- 230000001717 pathogenic effect Effects 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 2
- 239000010452 phosphate Substances 0.000 description 2
- 230000004962 physiological condition Effects 0.000 description 2
- 229920002401 polyacrylamide Polymers 0.000 description 2
- 229920001223 polyethylene glycol Polymers 0.000 description 2
- 229920002704 polyhistidine Polymers 0.000 description 2
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 2
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 2
- 238000001556 precipitation Methods 0.000 description 2
- 108060006633 protein kinase Proteins 0.000 description 2
- PYWVYCXTNDRMGF-UHFFFAOYSA-N rhodamine B Chemical compound [Cl-].C=12C=CC(=[N+](CC)CC)C=C2OC2=CC(N(CC)CC)=CC=C2C=1C1=CC=CC=C1C(O)=O PYWVYCXTNDRMGF-UHFFFAOYSA-N 0.000 description 2
- 229910000162 sodium phosphate Inorganic materials 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 238000010561 standard procedure Methods 0.000 description 2
- 235000000346 sugar Nutrition 0.000 description 2
- 150000008163 sugars Chemical class 0.000 description 2
- IIACRCGMVDHOTQ-UHFFFAOYSA-M sulfamate Chemical compound NS([O-])(=O)=O IIACRCGMVDHOTQ-UHFFFAOYSA-M 0.000 description 2
- NVBFHJWHLNUMCV-UHFFFAOYSA-N sulfamide Chemical compound NS(N)(=O)=O NVBFHJWHLNUMCV-UHFFFAOYSA-N 0.000 description 2
- 230000002194 synthesizing effect Effects 0.000 description 2
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 2
- 241000701161 unidentified adenovirus Species 0.000 description 2
- 102000040650 (ribonucleotides)n+m Human genes 0.000 description 1
- TVZRAEYQIKYCPH-UHFFFAOYSA-N 3-(trimethylsilyl)propane-1-sulfonic acid Chemical compound C[Si](C)(C)CCCS(O)(=O)=O TVZRAEYQIKYCPH-UHFFFAOYSA-N 0.000 description 1
- 102000007469 Actins Human genes 0.000 description 1
- 108010085238 Actins Proteins 0.000 description 1
- 208000010370 Adenoviridae Infections Diseases 0.000 description 1
- 206010060931 Adenovirus infection Diseases 0.000 description 1
- 102000002260 Alkaline Phosphatase Human genes 0.000 description 1
- 108020004774 Alkaline Phosphatase Proteins 0.000 description 1
- 244000105975 Antidesma platyphyllum Species 0.000 description 1
- 108020005544 Antisense RNA Proteins 0.000 description 1
- 235000017060 Arachis glabrata Nutrition 0.000 description 1
- 244000105624 Arachis hypogaea Species 0.000 description 1
- 235000010777 Arachis hypogaea Nutrition 0.000 description 1
- 235000018262 Arachis monticola Nutrition 0.000 description 1
- 241000972773 Aulopiformes Species 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 108020004635 Complementary DNA Proteins 0.000 description 1
- 108010062580 Concanavalin A Proteins 0.000 description 1
- 201000003883 Cystic fibrosis Diseases 0.000 description 1
- 239000003155 DNA primer Substances 0.000 description 1
- 230000028937 DNA protection Effects 0.000 description 1
- 102000052510 DNA-Binding Proteins Human genes 0.000 description 1
- 108700020911 DNA-Binding Proteins Proteins 0.000 description 1
- 241000276484 Gadus ogac Species 0.000 description 1
- 102000002812 Heat-Shock Proteins Human genes 0.000 description 1
- 108010004889 Heat-Shock Proteins Proteins 0.000 description 1
- 241000282412 Homo Species 0.000 description 1
- 101000818543 Homo sapiens Tyrosine-protein kinase ZAP-70 Proteins 0.000 description 1
- 101710203526 Integrase Proteins 0.000 description 1
- 241000239218 Limulus Species 0.000 description 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
- 101100268066 Mus musculus Zap70 gene Proteins 0.000 description 1
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical class CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 description 1
- 239000000020 Nitrocellulose Substances 0.000 description 1
- 239000004677 Nylon Substances 0.000 description 1
- 102000001490 Opioid Peptides Human genes 0.000 description 1
- 108010093625 Opioid Peptides Proteins 0.000 description 1
- 238000002944 PCR assay Methods 0.000 description 1
- 108020002230 Pancreatic Ribonuclease Proteins 0.000 description 1
- 102000005891 Pancreatic ribonuclease Human genes 0.000 description 1
- 241000219833 Phaseolus Species 0.000 description 1
- 239000004743 Polypropylene Substances 0.000 description 1
- 102000009516 Protein Serine-Threonine Kinases Human genes 0.000 description 1
- 108010009341 Protein Serine-Threonine Kinases Proteins 0.000 description 1
- 108010046983 Ribonuclease T1 Proteins 0.000 description 1
- 101150001535 SRC gene Proteins 0.000 description 1
- 229910000831 Steel Inorganic materials 0.000 description 1
- 108020004566 Transfer RNA Proteins 0.000 description 1
- 239000007984 Tris EDTA buffer Substances 0.000 description 1
- 241000209140 Triticum Species 0.000 description 1
- 235000021307 Triticum Nutrition 0.000 description 1
- 102000004243 Tubulin Human genes 0.000 description 1
- 108090000704 Tubulin Proteins 0.000 description 1
- 102100021125 Tyrosine-protein kinase ZAP-70 Human genes 0.000 description 1
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 1
- 240000004922 Vigna radiata Species 0.000 description 1
- 235000010721 Vigna radiata var radiata Nutrition 0.000 description 1
- 235000011469 Vigna radiata var sublobata Nutrition 0.000 description 1
- CESGKXMBHGUQTB-VONOSFMSSA-N [(1S,2S,6R,10S,11R,13S,14R,15R)-1,6,14-trihydroxy-8-(hydroxymethyl)-4,12,12,15-tetramethyl-5-oxo-13-tetracyclo[8.5.0.02,6.011,13]pentadeca-3,8-dienyl] tetradecanoate Chemical compound C1=C(CO)C[C@]2(O)C(=O)C(C)=C[C@H]2[C@@]2(O)[C@H](C)[C@@H](O)[C@@]3(OC(=O)CCCCCCCCCCCCC)C(C)(C)[C@H]3[C@@H]21 CESGKXMBHGUQTB-VONOSFMSSA-N 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 238000000862 absorption spectrum Methods 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 208000011589 adenoviridae infectious disease Diseases 0.000 description 1
- 238000005377 adsorption chromatography Methods 0.000 description 1
- 238000001042 affinity chromatography Methods 0.000 description 1
- 239000000910 agglutinin Substances 0.000 description 1
- 150000001299 aldehydes Chemical class 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 238000005904 alkaline hydrolysis reaction Methods 0.000 description 1
- 229910052782 aluminium Inorganic materials 0.000 description 1
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 1
- 150000001408 amides Chemical class 0.000 description 1
- 150000001412 amines Chemical class 0.000 description 1
- 239000002269 analeptic agent Substances 0.000 description 1
- 238000004873 anchoring Methods 0.000 description 1
- 230000000890 antigenic effect Effects 0.000 description 1
- 239000002246 antineoplastic agent Substances 0.000 description 1
- 238000003782 apoptosis assay Methods 0.000 description 1
- 230000006907 apoptotic process Effects 0.000 description 1
- 238000000149 argon plasma sintering Methods 0.000 description 1
- 238000011948 assay development Methods 0.000 description 1
- 238000003149 assay kit Methods 0.000 description 1
- 238000000376 autoradiography Methods 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 238000012742 biochemical analysis Methods 0.000 description 1
- 238000010256 biochemical assay Methods 0.000 description 1
- 238000005842 biochemical reaction Methods 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 239000004202 carbamide Substances 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 1
- 239000003054 catalyst Substances 0.000 description 1
- 230000030833 cell death Effects 0.000 description 1
- 108091092328 cellular RNA Proteins 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 239000000919 ceramic Substances 0.000 description 1
- 229910010293 ceramic material Inorganic materials 0.000 description 1
- 238000002144 chemical decomposition reaction Methods 0.000 description 1
- 125000003636 chemical group Chemical group 0.000 description 1
- 238000002038 chemiluminescence detection Methods 0.000 description 1
- 238000004737 colorimetric analysis Methods 0.000 description 1
- 230000006957 competitive inhibition Effects 0.000 description 1
- 238000004590 computer program Methods 0.000 description 1
- 238000001218 confocal laser scanning microscopy Methods 0.000 description 1
- 238000004132 cross linking Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 125000004122 cyclic group Chemical group 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 239000003398 denaturant Substances 0.000 description 1
- 238000004925 denaturation Methods 0.000 description 1
- 230000036425 denaturation Effects 0.000 description 1
- 238000003795 desorption Methods 0.000 description 1
- 230000023077 detection of light stimulus Effects 0.000 description 1
- 238000002405 diagnostic procedure Methods 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 238000002408 directed self-assembly Methods 0.000 description 1
- BFMYDTVEBKDAKJ-UHFFFAOYSA-L disodium;(2',7'-dibromo-3',6'-dioxido-3-oxospiro[2-benzofuran-1,9'-xanthene]-4'-yl)mercury;hydrate Chemical compound O.[Na+].[Na+].O1C(=O)C2=CC=CC=C2C21C1=CC(Br)=C([O-])C([Hg])=C1OC1=C2C=C(Br)C([O-])=C1 BFMYDTVEBKDAKJ-UHFFFAOYSA-L 0.000 description 1
- UKWLRLAKGMZXJC-QIECWBMSSA-L disodium;[4-chloro-3-[(3r,5s)-1-chloro-3'-methoxyspiro[adamantane-4,4'-dioxetane]-3'-yl]phenyl] phosphate Chemical compound [Na+].[Na+].O1OC2([C@@H]3CC4C[C@H]2CC(Cl)(C4)C3)C1(OC)C1=CC(OP([O-])([O-])=O)=CC=C1Cl UKWLRLAKGMZXJC-QIECWBMSSA-L 0.000 description 1
- 239000012636 effector Substances 0.000 description 1
- 238000010291 electrical method Methods 0.000 description 1
- 238000005401 electroluminescence Methods 0.000 description 1
- 239000012149 elution buffer Substances 0.000 description 1
- 230000013020 embryo development Effects 0.000 description 1
- 238000000295 emission spectrum Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 238000006911 enzymatic reaction Methods 0.000 description 1
- 238000001952 enzyme assay Methods 0.000 description 1
- 238000005530 etching Methods 0.000 description 1
- 238000012869 ethanol precipitation Methods 0.000 description 1
- DEFVIWRASFVYLL-UHFFFAOYSA-N ethylene glycol bis(2-aminoethyl)tetraacetic acid Chemical compound OC(=O)CN(CC(O)=O)CCOCCOCCN(CC(O)=O)CC(O)=O DEFVIWRASFVYLL-UHFFFAOYSA-N 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 238000000695 excitation spectrum Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000002866 fluorescence resonance energy transfer Methods 0.000 description 1
- 229910052731 fluorine Inorganic materials 0.000 description 1
- 239000011737 fluorine Substances 0.000 description 1
- 125000000524 functional group Chemical group 0.000 description 1
- 238000001502 gel electrophoresis Methods 0.000 description 1
- 102000034356 gene-regulatory proteins Human genes 0.000 description 1
- 108091006104 gene-regulatory proteins Proteins 0.000 description 1
- 230000009395 genetic defect Effects 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 230000007614 genetic variation Effects 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 108091005608 glycosylated proteins Proteins 0.000 description 1
- 102000035122 glycosylated proteins Human genes 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 235000009424 haa Nutrition 0.000 description 1
- 238000012203 high throughput assay Methods 0.000 description 1
- 238000012188 high-throughput screening assay Methods 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 108091008039 hormone receptors Proteins 0.000 description 1
- 239000005457 ice water Substances 0.000 description 1
- 238000012606 in vitro cell culture Methods 0.000 description 1
- 238000011065 in-situ storage Methods 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 230000028709 inflammatory response Effects 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 230000000977 initiatory effect Effects 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 206010022498 insulinoma Diseases 0.000 description 1
- 230000002427 irreversible effect Effects 0.000 description 1
- 238000005304 joining Methods 0.000 description 1
- 229910052747 lanthanoid Inorganic materials 0.000 description 1
- 230000004807 localization Effects 0.000 description 1
- UEGPKNKPLBYCNK-UHFFFAOYSA-L magnesium acetate Chemical compound [Mg+2].CC([O-])=O.CC([O-])=O UEGPKNKPLBYCNK-UHFFFAOYSA-L 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 102000006240 membrane receptors Human genes 0.000 description 1
- 108020004084 membrane receptors Proteins 0.000 description 1
- 230000005499 meniscus Effects 0.000 description 1
- 229910021645 metal ion Inorganic materials 0.000 description 1
- MYWUZJCMWCOHBA-VIFPVBQESA-N methamphetamine Chemical compound CN[C@@H](C)CC1=CC=CC=C1 MYWUZJCMWCOHBA-VIFPVBQESA-N 0.000 description 1
- 238000005459 micromachining Methods 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 238000010369 molecular cloning Methods 0.000 description 1
- 230000004001 molecular interaction Effects 0.000 description 1
- 239000003068 molecular probe Substances 0.000 description 1
- 235000019799 monosodium phosphate Nutrition 0.000 description 1
- 230000009826 neoplastic cell growth Effects 0.000 description 1
- 239000002858 neurotransmitter agent Substances 0.000 description 1
- 229920001220 nitrocellulos Polymers 0.000 description 1
- 230000006959 non-competitive inhibition Effects 0.000 description 1
- 230000009871 nonspecific binding Effects 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 238000010606 normalization Methods 0.000 description 1
- 229920001778 nylon Polymers 0.000 description 1
- 238000002966 oligonucleotide array Methods 0.000 description 1
- 150000002482 oligosaccharides Polymers 0.000 description 1
- 239000003399 opiate peptide Substances 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 210000003463 organelle Anatomy 0.000 description 1
- 208000021255 pancreatic insulinoma Diseases 0.000 description 1
- 239000000123 paper Substances 0.000 description 1
- 238000005192 partition Methods 0.000 description 1
- 230000001575 pathological effect Effects 0.000 description 1
- 230000007170 pathology Effects 0.000 description 1
- 239000013610 patient sample Substances 0.000 description 1
- 238000000059 patterning Methods 0.000 description 1
- 235000020232 peanut Nutrition 0.000 description 1
- 238000011170 pharmaceutical development Methods 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- 125000002467 phosphate group Chemical group [H]OP(=O)(O[H])O[*] 0.000 description 1
- 229940067631 phospholipid Drugs 0.000 description 1
- 150000003904 phospholipids Chemical class 0.000 description 1
- DCWXELXMIBXGTH-QMMMGPOBSA-N phosphonotyrosine Chemical group OC(=O)[C@@H](N)CC1=CC=C(OP(O)(O)=O)C=C1 DCWXELXMIBXGTH-QMMMGPOBSA-N 0.000 description 1
- 108091005981 phosphorylated proteins Proteins 0.000 description 1
- 239000002504 physiological saline solution Substances 0.000 description 1
- 239000013612 plasmid Substances 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 229920001155 polypropylene Polymers 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 230000034918 positive regulation of cell growth Effects 0.000 description 1
- 229940124606 potential therapeutic agent Drugs 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 150000003141 primary amines Chemical class 0.000 description 1
- 230000005522 programmed cell death Effects 0.000 description 1
- 108020001580 protein domains Proteins 0.000 description 1
- 150000003222 pyridines Chemical class 0.000 description 1
- WVIICGIFSIBFOG-UHFFFAOYSA-N pyrylium Chemical class C1=CC=[O+]C=C1 WVIICGIFSIBFOG-UHFFFAOYSA-N 0.000 description 1
- 239000010453 quartz Substances 0.000 description 1
- 230000002285 radioactive effect Effects 0.000 description 1
- 238000001525 receptor binding assay Methods 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000011514 reflex Effects 0.000 description 1
- 102000037983 regulatory factors Human genes 0.000 description 1
- 108091008025 regulatory factors Proteins 0.000 description 1
- 230000010076 replication Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 238000003757 reverse transcription PCR Methods 0.000 description 1
- 230000002441 reversible effect Effects 0.000 description 1
- 235000019515 salmon Nutrition 0.000 description 1
- 238000009738 saturating Methods 0.000 description 1
- 238000004621 scanning probe microscopy Methods 0.000 description 1
- 238000002821 scintillation proximity assay Methods 0.000 description 1
- 238000012106 screening analysis Methods 0.000 description 1
- 229910052814 silicon oxide Inorganic materials 0.000 description 1
- 239000002210 silicon-based material Substances 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- AJPJDKMHJJGVTQ-UHFFFAOYSA-M sodium dihydrogen phosphate Chemical compound [Na+].OP(O)([O-])=O AJPJDKMHJJGVTQ-UHFFFAOYSA-M 0.000 description 1
- 239000001488 sodium phosphate Substances 0.000 description 1
- 235000011008 sodium phosphates Nutrition 0.000 description 1
- 239000007790 solid phase Substances 0.000 description 1
- 230000007480 spreading Effects 0.000 description 1
- 238000003892 spreading Methods 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 239000010959 steel Substances 0.000 description 1
- 150000003431 steroids Chemical class 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000002198 surface plasmon resonance spectroscopy Methods 0.000 description 1
- 125000005207 tetraalkylammonium group Chemical group 0.000 description 1
- 229940124597 therapeutic agent Drugs 0.000 description 1
- 229940126585 therapeutic drug Drugs 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 239000003053 toxin Substances 0.000 description 1
- 231100000765 toxin Toxicity 0.000 description 1
- 108700012359 toxins Proteins 0.000 description 1
- 230000008733 trauma Effects 0.000 description 1
- RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 description 1
- 230000004614 tumor growth Effects 0.000 description 1
- 239000000717 tumor promoter Substances 0.000 description 1
- 238000009281 ultraviolet germicidal irradiation Methods 0.000 description 1
- 239000002435 venom Substances 0.000 description 1
- 231100000611 venom Toxicity 0.000 description 1
- 210000001048 venom Anatomy 0.000 description 1
- 238000012800 visualization Methods 0.000 description 1
- 239000001993 wax Substances 0.000 description 1
- 238000001262 western blot Methods 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B30/00—Methods of screening libraries
- C40B30/04—Methods of screening libraries by measuring the ability to specifically bind a target molecule, e.g. antibody-antigen binding, receptor-ligand binding
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00583—Features relative to the processes being carried out
- B01J2219/00603—Making arrays on substantially continuous surfaces
- B01J2219/00659—Two-dimensional arrays
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00583—Features relative to the processes being carried out
- B01J2219/00603—Making arrays on substantially continuous surfaces
- B01J2219/00659—Two-dimensional arrays
- B01J2219/00662—Two-dimensional arrays within two-dimensional arrays
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00718—Type of compounds synthesised
- B01J2219/0072—Organic compounds
- B01J2219/00722—Nucleotides
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B40/00—Libraries per se, e.g. arrays, mixtures
- C40B40/04—Libraries containing only organic compounds
- C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Biochemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- General Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Engineering & Computer Science (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
- Signal Processing For Digital Recording And Reproducing (AREA)
- Fats And Perfumes (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US6829197P | 1997-12-19 | 1997-12-19 | |
US09/109,076 US6232066B1 (en) | 1997-12-19 | 1998-07-02 | High throughput assay system |
PCT/US1998/027191 WO1999032663A2 (fr) | 1997-12-19 | 1998-12-21 | Systeme de dosage a haut rendement |
Publications (3)
Publication Number | Publication Date |
---|---|
NO20003115D0 NO20003115D0 (no) | 2000-06-16 |
NO20003115L NO20003115L (no) | 2000-08-21 |
NO329977B1 true NO329977B1 (no) | 2011-01-31 |
Family
ID=26748811
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
NO20003115A NO329977B1 (no) | 1997-12-19 | 2000-06-16 | Fremgangsmate for pavisning av minst ett nukleinsyremal og sett for pavisning av minst ett nukleinsyremal i en prove |
Country Status (19)
Country | Link |
---|---|
US (1) | US6232066B1 (fr) |
EP (1) | EP1038033B1 (fr) |
JP (5) | JP2001526904A (fr) |
KR (1) | KR100708782B1 (fr) |
CN (1) | CN1292076C (fr) |
AT (1) | ATE392485T1 (fr) |
AU (2) | AU1936699A (fr) |
CA (1) | CA2315776C (fr) |
CZ (1) | CZ302381B6 (fr) |
DE (1) | DE69839373T2 (fr) |
DK (1) | DK1038033T3 (fr) |
EA (1) | EA006425B1 (fr) |
ES (1) | ES2306484T3 (fr) |
IL (2) | IL136862A0 (fr) |
MX (1) | MXPA00006058A (fr) |
NO (1) | NO329977B1 (fr) |
NZ (1) | NZ505319A (fr) |
PL (1) | PL193762B1 (fr) |
WO (1) | WO1999032663A2 (fr) |
Families Citing this family (212)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6720149B1 (en) * | 1995-06-07 | 2004-04-13 | Affymetrix, Inc. | Methods for concurrently processing multiple biological chip assays |
US7041510B2 (en) * | 1996-04-25 | 2006-05-09 | Bioarray Solutions Ltd. | System and method for programmable illumination pattern generation |
CA2255599C (fr) | 1996-04-25 | 2006-09-05 | Bioarray Solutions, Llc | Assemblage electrocinetique de particules proches des surfaces regule par la lumiere |
EP0990142A4 (fr) * | 1996-12-31 | 2000-09-27 | Genometrix Genomics Inc | Procede et dispositif d'analyse moleculaire multiplexee |
US20030096232A1 (en) | 1997-12-19 | 2003-05-22 | Kris Richard M. | High throughput assay system |
US6232066B1 (en) | 1997-12-19 | 2001-05-15 | Neogen, Inc. | High throughput assay system |
US6238869B1 (en) * | 1997-12-19 | 2001-05-29 | High Throughput Genomics, Inc. | High throughput assay system |
US20100105572A1 (en) * | 1997-12-19 | 2010-04-29 | Kris Richard M | High throughput assay system |
US20030039967A1 (en) * | 1997-12-19 | 2003-02-27 | Kris Richard M. | High throughput assay system using mass spectrometry |
EP2189791A3 (fr) | 1998-02-04 | 2011-03-09 | Life Technologies Corporation | Microréseaux et leurs utilisations |
EP2360271A1 (fr) | 1998-06-24 | 2011-08-24 | Illumina, Inc. | Décodage de capteurs de réseau avec des microsphères |
US20030138973A1 (en) * | 1998-07-14 | 2003-07-24 | Peter Wagner | Microdevices for screening biomolecules |
US6576478B1 (en) * | 1998-07-14 | 2003-06-10 | Zyomyx, Inc. | Microdevices for high-throughput screening of biomolecules |
US6780582B1 (en) * | 1998-07-14 | 2004-08-24 | Zyomyx, Inc. | Arrays of protein-capture agents and methods of use thereof |
US20020119579A1 (en) * | 1998-07-14 | 2002-08-29 | Peter Wagner | Arrays devices and methods of use thereof |
US6406921B1 (en) * | 1998-07-14 | 2002-06-18 | Zyomyx, Incorporated | Protein arrays for high-throughput screening |
US6908770B1 (en) * | 1998-07-16 | 2005-06-21 | Board Of Regents, The University Of Texas System | Fluid based analysis of multiple analytes by a sensor array |
US6950752B1 (en) * | 1998-10-27 | 2005-09-27 | Rosetta Inpharmatics Llc | Methods for removing artifact from biological profiles |
US6468476B1 (en) * | 1998-10-27 | 2002-10-22 | Rosetta Inpharmatics, Inc. | Methods for using-co-regulated genesets to enhance detection and classification of gene expression patterns |
AU2375600A (en) * | 1998-12-22 | 2000-07-12 | Stephen Felder | High throughput assay system using mass spectrometry |
US7612020B2 (en) * | 1998-12-28 | 2009-11-03 | Illumina, Inc. | Composite arrays utilizing microspheres with a hybridization chamber |
AU4058100A (en) * | 1999-04-09 | 2000-11-14 | Arcturus Engineering, Inc. | Generic cdna or protein array for customized assays |
US6221653B1 (en) * | 1999-04-27 | 2001-04-24 | Agilent Technologies, Inc. | Method of performing array-based hybridization assays using thermal inkjet deposition of sample fluids |
US6395483B1 (en) * | 1999-09-02 | 2002-05-28 | 3M Innovative Properties Company | Arrays with mask layers |
US6358557B1 (en) * | 1999-09-10 | 2002-03-19 | Sts Biopolymers, Inc. | Graft polymerization of substrate surfaces |
US7244559B2 (en) * | 1999-09-16 | 2007-07-17 | 454 Life Sciences Corporation | Method of sequencing a nucleic acid |
EP2343128A1 (fr) * | 1999-09-17 | 2011-07-13 | BioArray Solutions Ltd. | Substrat et chip pour conduire des essais biologiques |
US20030036070A1 (en) * | 1999-10-21 | 2003-02-20 | Shukti Chakravarti | Gene expression profiling of inflammatory bowel disease |
US6656432B1 (en) * | 1999-10-22 | 2003-12-02 | Ngk Insulators, Ltd. | Micropipette and dividedly injectable apparatus |
US6428957B1 (en) * | 1999-11-08 | 2002-08-06 | Agilent Technologies, Inc. | Systems tools and methods of assaying biological materials using spatially-addressable arrays |
JP4873812B2 (ja) * | 1999-11-15 | 2012-02-08 | キアジェン ゲイサーズバーグ インコーポレイテッド | マイクロアレイ上のrna:dnaハイブリッドの免疫検出 |
MXPA02006095A (es) * | 1999-12-21 | 2002-12-13 | Johnson & Johnson | Deteccion de acidos nucleicos. |
EP1257664A4 (fr) * | 2000-01-28 | 2006-04-05 | Althea Technologies Inc | Procedes d'analyse de l'expression genique |
EP1257805B1 (fr) * | 2000-02-10 | 2015-10-14 | Illumina, Inc. | Composant comprenant un substrat ayant une pluralité d'emplacements pour l'analyse simultanée de plusieurs échantillons a base de microspheres, dispositif comprenant le composant, et procédé de fabrication pour le composant |
US6770441B2 (en) * | 2000-02-10 | 2004-08-03 | Illumina, Inc. | Array compositions and methods of making same |
US20040014644A1 (en) * | 2000-03-14 | 2004-01-22 | Vladimir Efimov | Oligonucleotide analogues and methods of use for modulating gene expression |
CA2402319A1 (fr) | 2000-03-14 | 2001-09-20 | Active Motif | Analogues d'oligonucleotides, procedes de synthese et procedes de mise en oeuvre |
US6962906B2 (en) | 2000-03-14 | 2005-11-08 | Active Motif | Oligonucleotide analogues, methods of synthesis and methods of use |
CA2302827A1 (fr) * | 2000-04-12 | 2001-10-12 | Michelle Furtado | Denaturation de l'hybridation d'oligonucleotides et d'adn sur des surfaces |
US7338773B2 (en) * | 2000-04-14 | 2008-03-04 | Millipore Corporation | Multiplexed assays of cell migration |
WO2001083826A2 (fr) * | 2000-05-03 | 2001-11-08 | Massachusetts Institute Of Technology | Procedes et reactifs servant a assembler des molecules sur des supports solides |
TWI220927B (en) * | 2000-05-12 | 2004-09-11 | Rong-Seng Chang | Method for producing a micro-carrier |
US9709559B2 (en) | 2000-06-21 | 2017-07-18 | Bioarray Solutions, Ltd. | Multianalyte molecular analysis using application-specific random particle arrays |
AU2001272993B2 (en) | 2000-06-21 | 2005-03-10 | Bioarray Solutions, Ltd. | Multianalyte molecular analysis |
CN1137999C (zh) * | 2000-07-04 | 2004-02-11 | 清华大学 | 集成式微阵列装置 |
US6913879B1 (en) * | 2000-07-10 | 2005-07-05 | Telechem International Inc. | Microarray method of genotyping multiple samples at multiple LOCI |
AU2001278968A1 (en) * | 2000-07-19 | 2002-01-30 | Pointilliste, Inc. | Nested sorting and high throughput screening |
US7678539B2 (en) * | 2000-08-10 | 2010-03-16 | Corning Incorporated | Arrays of biological membranes and methods and use thereof |
CA2419544A1 (fr) * | 2000-08-14 | 2002-02-21 | Surface Logix, Inc. | Voies biomoleculaires |
EP1387894A4 (fr) * | 2000-08-24 | 2006-10-11 | Aviva Biosciences Corp | Procedes et compositions d'identification de molecules d'acide nucleique au moyen d'activites nucleolytiques et d'hybridation |
US6900013B1 (en) * | 2000-08-25 | 2005-05-31 | Aviva Biosciences Corporation | Methods and compositions for identifying nucleic acid molecules using nucleolytic activities and hybridization |
US20040185464A1 (en) * | 2000-09-15 | 2004-09-23 | Kris Richard M. | High throughput assay system |
US20020081589A1 (en) * | 2000-10-12 | 2002-06-27 | Jing-Shan Hu | Gene expression monitoring using universal arrays |
AU2001297830A1 (en) * | 2000-10-25 | 2002-12-09 | Exiqon A/S | Closed substrate platforms suitable for analysis of biomolecules |
US20020081617A1 (en) * | 2000-11-08 | 2002-06-27 | Tione Buranda | Fluorescence and FRET based assays for biomolecules on beads |
US6699665B1 (en) * | 2000-11-08 | 2004-03-02 | Surface Logix, Inc. | Multiple array system for integrating bioarrays |
US7351575B2 (en) * | 2000-11-08 | 2008-04-01 | Surface Logix, Inc. | Methods for processing biological materials using peelable and resealable devices |
US6937323B2 (en) | 2000-11-08 | 2005-08-30 | Burstein Technologies, Inc. | Interactive system for analyzing biological samples and processing related information and the use thereof |
US6905881B2 (en) | 2000-11-30 | 2005-06-14 | Paul Sammak | Microbead-based test plates and test methods for fluorescence imaging systems |
US7054258B2 (en) | 2000-12-08 | 2006-05-30 | Nagaoka & Co., Ltd. | Optical disc assemblies for performing assays |
WO2002046762A2 (fr) | 2000-12-08 | 2002-06-13 | Burstein Technologies, Inc. | Ensembles disques optiques pour l'execution de dosages biologiques |
US7079468B2 (en) | 2000-12-08 | 2006-07-18 | Burstein Technologies, Inc. | Optical discs for measuring analytes |
US6760298B2 (en) | 2000-12-08 | 2004-07-06 | Nagaoka & Co., Ltd. | Multiple data layer optical discs for detecting analytes |
US7776571B2 (en) * | 2000-12-12 | 2010-08-17 | Autogenomics, Inc. | Multi-substrate biochip unit |
US6869766B2 (en) * | 2000-12-22 | 2005-03-22 | The Regents Of The University Of California | Gene associated with regulation of adiposity and insulin response |
WO2002051537A2 (fr) * | 2000-12-22 | 2002-07-04 | Burstein Technologies, Inc. | Ensemble superficiel d'immobilisation de sondes de capture d'adn, et dosages a base de perles comprenant des biodisques optiques, et procede s'y rapportant |
US20090137416A1 (en) | 2001-01-16 | 2009-05-28 | Regeneron Pharmaceuticals, Inc. | Isolating Cells Expressing Secreted Proteins |
US6949638B2 (en) * | 2001-01-29 | 2005-09-27 | Affymetrix, Inc. | Photolithographic method and system for efficient mask usage in manufacturing DNA arrays |
AU2002255515A1 (en) * | 2001-02-05 | 2002-08-19 | Board Of Regents, The University Of Texas System | The use of mesoscale self-assembly and recognition to effect delivery of sensing reagent for arrayed sensors |
JP2002251091A (ja) * | 2001-02-27 | 2002-09-06 | Konica Corp | 画像定着装置および画像形成装置 |
AU2002307090A1 (en) * | 2001-04-03 | 2002-10-21 | Surromed, Inc. | Methods and reagents for multiplexed analyte capture, surface array self-assembly, and analysis of complex biological samples |
US20030170623A1 (en) * | 2001-04-13 | 2003-09-11 | Jingwen Chen | Multiplexed gene analysis on a mobile solid support |
US7572642B2 (en) * | 2001-04-18 | 2009-08-11 | Ambrigen, Llc | Assay based on particles, which specifically bind with targets in spatially distributed characteristic patterns |
KR20020089746A (ko) * | 2001-05-24 | 2002-11-30 | 주식회사 커벡스 | 크기변형유도 앵커 시발체, 이를 이용한 알티-피씨알 방법및 이를 이용한 진단 방법 |
US20030096264A1 (en) * | 2001-06-18 | 2003-05-22 | Psychiatric Genomics, Inc. | Multi-parameter high throughput screening assays (MPHTS) |
US20060234231A1 (en) * | 2001-06-20 | 2006-10-19 | Nuevolution A/S | Microarrays displaying encoded molecules |
WO2002102820A1 (fr) * | 2001-06-20 | 2002-12-27 | Nuevolution A/S | Derives nucleosidiques pour elaboration de bibliotheque combinatoire |
US7262063B2 (en) | 2001-06-21 | 2007-08-28 | Bio Array Solutions, Ltd. | Directed assembly of functional heterostructures |
WO2003004993A2 (fr) * | 2001-07-06 | 2003-01-16 | Millipore Corporation | Membrane composite a motifs et son procede de fabrication au pochoir |
US7141416B2 (en) | 2001-07-12 | 2006-11-28 | Burstein Technologies, Inc. | Multi-purpose optical analysis optical bio-disc for conducting assays and various reporting agents for use therewith |
AU2002367825A1 (en) * | 2001-07-30 | 2003-10-13 | Sts Biopolymers, Inc. | Graft polymer matrices |
US20050048566A1 (en) * | 2001-08-27 | 2005-03-03 | Delisi Charles | Apparatus, composition and method for proteome profiling |
US20030044320A1 (en) * | 2001-08-31 | 2003-03-06 | Shun Luo | High throughput screening micro array platform |
GB0124338D0 (en) * | 2001-10-10 | 2001-11-28 | Randox Lab Ltd | Biochips |
CA2741049C (fr) | 2001-10-15 | 2019-02-05 | Bioarray Solutions, Ltd. | Analyse multiplexee de loci polymorphique par detection indirecte de sonde |
AU2002363076A1 (en) * | 2001-10-26 | 2003-05-06 | Virtual Arrays, Inc. | Assay systems with adjustable fluid communication |
US7381375B2 (en) * | 2001-10-26 | 2008-06-03 | Millipore Corporation | Assay systems with adjustable fluid communication |
US20080187949A1 (en) * | 2001-10-26 | 2008-08-07 | Millipore Corporation | Multiplexed assays of cell migration |
AU2002360361A1 (en) | 2001-11-09 | 2003-06-10 | Biomicroarrays, Inc. | High surface area substrates for microarrays and methods to make same |
EP2196544A1 (fr) * | 2001-11-21 | 2010-06-16 | Applied Biosystems, LLC | Réactions de détection de ligature utilisant des marqueurs codés |
KR20030049802A (ko) * | 2001-12-17 | 2003-06-25 | 권윤정 | 바이오 칩에서 프로브와 시료 핵산의 혼성화 여부를 효소반응으로 구별하는 방법 |
US20060228723A1 (en) * | 2002-01-16 | 2006-10-12 | Keith Bradley | System and method for electronic sensing of biomolecules |
US20070178477A1 (en) * | 2002-01-16 | 2007-08-02 | Nanomix, Inc. | Nanotube sensor devices for DNA detection |
US6989235B2 (en) * | 2002-02-13 | 2006-01-24 | Motorola, Inc. | Single molecule detection of bio-agents using the F1-ATPase biomolecular motor |
EP2186897B1 (fr) * | 2002-03-15 | 2016-02-17 | Nuevolution A/S | Procédé amélioré pour la synthèse de molecules matricées |
AU2003228711C1 (en) * | 2002-04-26 | 2010-01-07 | Board Of Regents, The University Of Texas System | Method and system for the detection of cardiac risk factors |
US20040005612A1 (en) * | 2002-05-14 | 2004-01-08 | Giudice Linda C. | Endometrial genes in endometrial disorders |
US6841379B2 (en) | 2002-05-15 | 2005-01-11 | Beckman Coulter, Inc. | Conductive microplate |
JP4544989B2 (ja) * | 2002-05-24 | 2010-09-15 | ニンブルゲン システムズ インコーポレイテッド | 単一マイクロアレイ上で多数の試料のハイブリダイゼーション反応を行うマイクロアレイ及びその方法 |
US20030232384A1 (en) * | 2002-06-13 | 2003-12-18 | Eastman Kodak Company | Microarray system utilizing microtiter plates |
US9388459B2 (en) * | 2002-06-17 | 2016-07-12 | Affymetrix, Inc. | Methods for genotyping |
US7108976B2 (en) * | 2002-06-17 | 2006-09-19 | Affymetrix, Inc. | Complexity management of genomic DNA by locus specific amplification |
US20040053399A1 (en) * | 2002-07-17 | 2004-03-18 | Rudolf Gilmanshin | Methods and compositions for analyzing polymers using chimeric tags |
EP1539980B1 (fr) * | 2002-08-01 | 2016-02-17 | Nuevolution A/S | Synthese en plusieurs etapes de molecules synthetisees |
WO2004028682A2 (fr) * | 2002-09-27 | 2004-04-08 | Carlsberg A/S | Matrice polymere a codage spatial |
US7459273B2 (en) * | 2002-10-04 | 2008-12-02 | Affymetrix, Inc. | Methods for genotyping selected polymorphism |
US20080207465A1 (en) * | 2002-10-28 | 2008-08-28 | Millipore Corporation | Assay systems with adjustable fluid communication |
ES2368215T3 (es) | 2002-10-30 | 2011-11-15 | Nuevolution A/S | Codificación enzimática. |
ATE536548T1 (de) * | 2002-11-14 | 2011-12-15 | Vivalis | Mikrowell-array-chip zum nachweis eines antigenspezifischen lymphozyten, verfahren zum nachweis eines antigenspezifischen lymphozyten und verfahren zur klonierung eines antigenrezeptorgens eines antigenspezifischen lymphozyten |
US7526114B2 (en) | 2002-11-15 | 2009-04-28 | Bioarray Solutions Ltd. | Analysis, secure access to, and transmission of array images |
AU2003293511A1 (en) * | 2002-12-11 | 2004-06-30 | Arizona Board Of Regents, Acting For And On Behalf Of, Arizona State University | Polarization-enhanced detector with gold nanorods for detecting nanoscale rotational motion and method therefor |
EP2175019A3 (fr) | 2002-12-19 | 2011-04-06 | Nuevolution A/S | Procédés de synthèse guidée à fonction et structure quasi-sélectives |
EP1597577A4 (fr) * | 2003-02-10 | 2007-02-21 | Pointilliste Inc | Reseaux a assemblage autonome et utilisation de ceux-ci |
US20070026397A1 (en) | 2003-02-21 | 2007-02-01 | Nuevolution A/S | Method for producing second-generation library |
US20040185499A1 (en) * | 2003-03-20 | 2004-09-23 | Jolley Michael E. | Method of epitope scanning using fluorescence polarization |
US20050064452A1 (en) * | 2003-04-25 | 2005-03-24 | Schmid Matthew J. | System and method for the detection of analytes |
US20050009066A1 (en) * | 2003-05-15 | 2005-01-13 | Connolly Dennis M. | Methods for localizing target molecules in a flowing fluid sample |
WO2004104922A2 (fr) * | 2003-05-16 | 2004-12-02 | Board Of Regents, The University Of Texas System | Technologie de reconnaissance d'une image et d'une partie d'image |
US9317922B2 (en) | 2003-05-16 | 2016-04-19 | Board Of Regents The University Of Texas System | Image and part recognition technology |
CA2529016A1 (fr) * | 2003-06-12 | 2004-12-23 | Accupath Diagnostic Laboratories, Inc. | Procede et systeme pour l'analyse d'echantillons de cellules de forte densite |
US20040265883A1 (en) * | 2003-06-27 | 2004-12-30 | Biocept, Inc. | mRNA expression analysis |
US8114978B2 (en) | 2003-08-05 | 2012-02-14 | Affymetrix, Inc. | Methods for genotyping selected polymorphism |
CA2536565A1 (fr) * | 2003-09-10 | 2005-05-12 | Althea Technologies, Inc. | Profilage d'expression au moyen de microreseaux |
DE602004023960D1 (de) | 2003-09-18 | 2009-12-17 | Nuevolution As | Methode zur Gewinnung struktureller Informationen kodierter Moleküle und zur Selektion von Verbindungen |
US7927796B2 (en) | 2003-09-18 | 2011-04-19 | Bioarray Solutions, Ltd. | Number coding for identification of subtypes of coded types of solid phase carriers |
AU2004276761B2 (en) | 2003-09-22 | 2009-12-24 | Bioarray Solutions, Ltd. | Surface immobilized polyelectrolyte with multiple functional groups capable of covalently bonding to biomolecules |
WO2005069001A1 (fr) * | 2003-09-25 | 2005-07-28 | Toyama Prefecture | Puce de reseau de micropuits et son procede de fabrication |
JP2007521017A (ja) | 2003-10-28 | 2007-08-02 | バイオアレイ ソリューションズ リミテッド | 固定化捕捉プローブを用いる遺伝子発現分析法の最適化 |
WO2005045060A2 (fr) | 2003-10-29 | 2005-05-19 | Bioarray Solutions, Ltd. | Analyse multiplexee d'acide nucleique par fragmentation d'adn bicatenaire |
WO2005050170A2 (fr) * | 2003-11-17 | 2005-06-02 | Auburn University | Procedes et compositions permettant d'inhiber la formation d'agregats intracellulaires |
AU2004300168A1 (en) * | 2003-12-11 | 2005-06-30 | Board Of Regents, The University Of Texas System | Method and system for the analysis of saliva using a sensor array |
US7419820B2 (en) * | 2003-12-16 | 2008-09-02 | Canon Kabushiki Kaisha | Transfer sheet for transferring biologically active substance to culture plate |
JP2007518107A (ja) * | 2004-01-13 | 2007-07-05 | ユー.エス. ジェノミクス, インコーポレイテッド | ポリマーを使用する溶液中の分析物の検出および定量化 |
WO2005078122A2 (fr) * | 2004-02-17 | 2005-08-25 | Nuevolution A/S | Methode d'elimination visant a un enrichissement par hybridation de mesappariements |
US8101431B2 (en) | 2004-02-27 | 2012-01-24 | Board Of Regents, The University Of Texas System | Integration of fluids and reagents into self-contained cartridges containing sensor elements and reagent delivery systems |
US8105849B2 (en) | 2004-02-27 | 2012-01-31 | Board Of Regents, The University Of Texas System | Integration of fluids and reagents into self-contained cartridges containing sensor elements |
JP2007529758A (ja) * | 2004-03-19 | 2007-10-25 | ユー.エス. ジェノミクス, インコーポレイテッド | 単一分子の検出のための組成物および方法 |
JP5435864B2 (ja) * | 2004-05-28 | 2014-03-05 | アシュラジェン インコーポレイテッド | マイクロrnaに関与する方法および組成物 |
US7848889B2 (en) | 2004-08-02 | 2010-12-07 | Bioarray Solutions, Ltd. | Automated analysis of multiplexed probe-target interaction patterns: pattern matching and allele identification |
CN100352937C (zh) * | 2004-08-06 | 2007-12-05 | 中国海洋大学 | 对藻类进行定性与定量分析的方法 |
JP2008511008A (ja) * | 2004-08-24 | 2008-04-10 | ナノミックス・インコーポレーテッド | Dna検出用ナノチューブセンサー装置 |
US20060078894A1 (en) * | 2004-10-12 | 2006-04-13 | Winkler Matthew M | Methods and compositions for analyzing nucleic acids |
DK2302055T3 (da) | 2004-11-12 | 2014-10-13 | Asuragen Inc | Fremgangsmåder og sammensætninger involverende miRNA og miRNA-inhibitormolekyler |
US20060246576A1 (en) | 2005-04-06 | 2006-11-02 | Affymetrix, Inc. | Fluidic system and method for processing biological microarrays in personal instrumentation |
US7452671B2 (en) | 2005-04-29 | 2008-11-18 | Affymetrix, Inc. | Methods for genotyping with selective adaptor ligation |
EP1883710A4 (fr) * | 2005-05-03 | 2009-07-08 | Althea Technologies Inc | Compositions et procedes d'analyse d'acides nucleiques degrades |
EP1910824A4 (fr) | 2005-05-31 | 2012-11-21 | Labnow Inc | Méthodes et compositions en rapport avec la détermination et l utilisation de la numération de globules blancs |
US8486629B2 (en) | 2005-06-01 | 2013-07-16 | Bioarray Solutions, Ltd. | Creation of functionalized microparticle libraries by oligonucleotide ligation or elongation |
US20070081163A1 (en) * | 2005-06-03 | 2007-04-12 | Minhua Liang | Method and apparatus for scanned beam microarray assay |
AU2006261953B2 (en) * | 2005-06-24 | 2012-02-23 | Board Of Regents, The University Of Texas System | Systems and methods including self-contained cartridges with detection systems and fluid delivery systems |
WO2007056250A2 (fr) * | 2005-11-04 | 2007-05-18 | U.S. Genomics, Inc. | Analyse heterogene d'analytes en solution au moyen de polymeres |
US7889347B2 (en) * | 2005-11-21 | 2011-02-15 | Plexera Llc | Surface plasmon resonance spectrometer with an actuator driven angle scanning mechanism |
DE602006018648D1 (de) | 2005-12-01 | 2011-01-13 | Nuevolution As | Enzymvermittelnde kodierungsmethoden für eine effiziente synthese von grossen bibliotheken |
US7463358B2 (en) * | 2005-12-06 | 2008-12-09 | Lumera Corporation | Highly stable surface plasmon resonance plates, microarrays, and methods |
US11306351B2 (en) | 2005-12-21 | 2022-04-19 | Affymetrix, Inc. | Methods for genotyping |
US20110027890A1 (en) * | 2005-12-26 | 2011-02-03 | Kuraray Co., Ltd. | Material for cell culture |
US20090176208A1 (en) * | 2006-01-04 | 2009-07-09 | Simon Brodie | Methods for detecting, identifying and reporting the presence of animal pathological agents |
US20080003667A1 (en) * | 2006-05-19 | 2008-01-03 | Affymetrix, Inc. | Consumable elements for use with fluid processing and detection systems |
EP2636755A1 (fr) * | 2006-05-26 | 2013-09-11 | AltheaDx Incorporated | Analyse biochimique de cellules divisées |
US9957157B2 (en) * | 2006-09-19 | 2018-05-01 | University of Houtson System | Fabrication of self assembling nano-structures |
CA2663962A1 (fr) * | 2006-09-19 | 2008-03-27 | Asuragen, Inc. | Genes regules mir-15, mir-26, mir -31,mir -145, mir-147, mir-188, mir-215, mir-216 mir-331, mmu-mir-292-3p et voies de signalisation utiles comme cibles dans une intervention therapeutique |
US20080131878A1 (en) * | 2006-12-05 | 2008-06-05 | Asuragen, Inc. | Compositions and Methods for the Detection of Small RNA |
EP2104737B1 (fr) * | 2006-12-08 | 2013-04-10 | Asuragen, INC. | Fonctions et cibles de microarn let-7 |
EP2104734A2 (fr) * | 2006-12-08 | 2009-09-30 | Asuragen, INC. | Gènes et voies génétiques régulés par le mir-20 en tant que cibles en vue d'une intervention thérapeutique |
CN101627121A (zh) * | 2006-12-08 | 2010-01-13 | 奥斯瑞根公司 | 作为治疗干预的靶标的miRNA调控基因和路径 |
AU2007333107A1 (en) * | 2006-12-08 | 2008-06-19 | Asuragen, Inc. | miR-21 regulated genes and pathways as targets for therapeutic intervention |
CA2671270A1 (fr) * | 2006-12-29 | 2008-07-17 | Asuragen, Inc. | Genes et voies regules par mir-16 utiles comme cibles pour intervention therapeutique |
US20090232893A1 (en) * | 2007-05-22 | 2009-09-17 | Bader Andreas G | miR-143 REGULATED GENES AND PATHWAYS AS TARGETS FOR THERAPEUTIC INTERVENTION |
US20080293589A1 (en) * | 2007-05-24 | 2008-11-27 | Affymetrix, Inc. | Multiplex locus specific amplification |
US20090227533A1 (en) * | 2007-06-08 | 2009-09-10 | Bader Andreas G | miR-34 Regulated Genes and Pathways as Targets for Therapeutic Intervention |
US20100294665A1 (en) * | 2007-07-12 | 2010-11-25 | Richard Allen | Method and system for transferring and/or concentrating a sample |
JP4148367B1 (ja) | 2007-08-02 | 2008-09-10 | 富山県 | 細胞のスクリーニング方法 |
US20090060786A1 (en) * | 2007-08-29 | 2009-03-05 | Gibum Kim | Microfluidic apparatus for wide area microarrays |
EP2198050A1 (fr) * | 2007-09-14 | 2010-06-23 | Asuragen, INC. | Microarn exprimés de manière différentielle dans le cancer du col de l'utérus et leurs utilisations |
US9388457B2 (en) * | 2007-09-14 | 2016-07-12 | Affymetrix, Inc. | Locus specific amplification using array probes |
WO2009052386A1 (fr) * | 2007-10-18 | 2009-04-23 | Asuragen, Inc. | Micro arn exprimés différentiellement dans des maladies pulmonaires et leurs utilisations |
US8071562B2 (en) * | 2007-12-01 | 2011-12-06 | Mirna Therapeutics, Inc. | MiR-124 regulated genes and pathways as targets for therapeutic intervention |
US8004669B1 (en) | 2007-12-18 | 2011-08-23 | Plexera Llc | SPR apparatus with a high performance fluid delivery system |
WO2009086156A2 (fr) * | 2007-12-21 | 2009-07-09 | Asuragen, Inc. | Gènes et voies régulés par mir-10 servant de cibles dans le cadre d'une intervention thérapeutique |
EP2260110B1 (fr) * | 2008-02-08 | 2014-11-12 | Asuragen, INC. | Micro arn (mirna) exprimés différentiellement dans des noeuds lymphoïdes prélevés chez des patients atteints d'un cancer |
US9074244B2 (en) | 2008-03-11 | 2015-07-07 | Affymetrix, Inc. | Array-based translocation and rearrangement assays |
WO2009154835A2 (fr) * | 2008-03-26 | 2009-12-23 | Asuragen, Inc. | Compositions et procédés liés à mir-16 et à la thérapie contre le cancer de la prostate |
WO2009123748A1 (fr) * | 2008-04-05 | 2009-10-08 | Single Cell Technology, Inc. | Procédé d'obtention d'anticorps d'intérêt et des nucléotides codant pour ceux-ci |
WO2009126726A1 (fr) * | 2008-04-08 | 2009-10-15 | Asuragen, Inc | Procédés et compositions pour diagnostiquer et moduler le papillomavirus humain (hpv) |
EP2285960B1 (fr) | 2008-05-08 | 2015-07-08 | Asuragen, INC. | Compositions et procédés liés à la modulation de miarn-184 de néovascularisation ou d angiogenèse |
US20110159492A1 (en) * | 2008-06-04 | 2011-06-30 | Rimsza Lisa M | Diffuse Large B-Cell Lymphoma Markers and Uses Therefore |
ES2721173T3 (es) * | 2008-11-07 | 2019-07-29 | Hoffmann La Roche | Sustancias de relleno de grano fino para películas de reacción fotométricas |
US20100179213A1 (en) * | 2008-11-11 | 2010-07-15 | Mirna Therapeutics, Inc. | Methods and Compositions Involving miRNAs In Cancer Stem Cells |
JP5745752B2 (ja) * | 2009-05-21 | 2015-07-08 | オリンパス株式会社 | 細胞スクリーニング方法、その方法に用いる制御ソフトウェア、細胞スクリーニング装置、及び画像解析装置、並びに細胞スクリーニングシステム |
JP2013509863A (ja) | 2009-11-03 | 2013-03-21 | エイチティージー モレキュラー ダイアグノスティクス, インコーポレイテッド | 定量的ヌクレアーゼ保護シークエンシング(qNPS) |
DK2558577T3 (en) | 2010-04-16 | 2019-04-01 | Nuevolution As | Bi-functional complexes and methods for the preparation and use of such complexes |
JP5951755B2 (ja) | 2011-05-04 | 2016-07-13 | エイチティージー モレキュラー ダイアグノスティクス, インコーポレイテッド | 定量的ヌクレアーゼプロテクションアッセイ(qNPA)法および定量的ヌクレアーゼプロテクション配列決定(qNPS)法の改善 |
ES2578370T3 (es) | 2011-07-01 | 2016-07-26 | HTG Molecular Diagnostics, Inc | Métodos para detectar fusiones génicas |
WO2013040251A2 (fr) | 2011-09-13 | 2013-03-21 | Asurgen, Inc. | Méthodes et compositions incluant mir-135b, permettant de faire la distinction entre un cancer du pancréas et une maladie pancréatique bénigne |
MY157586A (en) * | 2011-09-14 | 2016-06-16 | Ngk Insulators Ltd | Method for detecting target nucleic acid |
US20140198314A1 (en) * | 2011-10-18 | 2014-07-17 | Zhiyong Li | Molecular sensing device |
CN104603291B (zh) | 2012-06-22 | 2018-04-06 | Htg分子诊断有限公司 | 黑素细胞病变中的分子恶性肿瘤 |
EP2867373B1 (fr) | 2012-06-29 | 2018-12-19 | HTG Molecular Diagnostics, Inc. | Procédés de protection d'une nucléase pour la détection de variants nucléotidiques |
EP2917715B1 (fr) | 2012-11-06 | 2019-04-03 | Biodot, Inc. | Impression commandée d'échantillon de cellules pour caryotypage |
TWI745610B (zh) | 2012-11-14 | 2021-11-11 | 美商再生元醫藥公司 | 重組細胞表面捕捉蛋白質 |
WO2014150853A1 (fr) * | 2013-03-15 | 2014-09-25 | Inanovate, Inc. | Mesure d'analyte(s) à l'aide d'un dosage longitudinal |
WO2016089588A1 (fr) | 2014-12-06 | 2016-06-09 | Children's Medical Center Corporation | Séquences de liaison à base d'acides nucléiques utilisables en vue de la détection et de la mesure d'interactions |
US11396650B2 (en) | 2015-06-02 | 2022-07-26 | Children's Medical Center Corporation | Nucleic acid complexes for screening barcoded compounds |
US12077807B2 (en) * | 2015-06-27 | 2024-09-03 | The Research Foundation For The State University Of New York | Compositions and methods for analyte detection using nanoswitches |
US11384399B2 (en) | 2015-09-29 | 2022-07-12 | Htg Molecular Diagnostics, Inc. | Methods for subtyping diffuse large B-cell lymphoma (DLBCL) |
US11713483B2 (en) | 2016-02-09 | 2023-08-01 | Children's Medical Center Corporation | Method for detection of analytes via polymer complexes |
AU2017336153B2 (en) * | 2016-09-30 | 2023-07-13 | Guardant Health, Inc. | Methods for multi-resolution analysis of cell-free nucleic acids |
WO2019014185A1 (fr) * | 2017-07-10 | 2019-01-17 | The Charles Stark Draper Laboratory, Inc. | Appareil permettant un stockage d'informations de haute densité dans des chaînes moléculaires |
WO2019069372A1 (fr) * | 2017-10-03 | 2019-04-11 | 株式会社ニコン | Procédé de mesure de cible de détection, support d'ancrage de sonde de capture, kit de détection et dispositif de fluide |
WO2019100080A1 (fr) | 2017-11-20 | 2019-05-23 | Children's Medical Center Corporation | Dosages de nanocommutateur commandé par une force pour détection de molécule unique dans des liquides biologiques complexes |
US11995558B2 (en) | 2018-05-17 | 2024-05-28 | The Charles Stark Draper Laboratory, Inc. | Apparatus for high density information storage in molecular chains |
GB2599709A (en) * | 2020-10-09 | 2022-04-13 | Qbd Qs Ip Ltd | Analysis system and method |
Family Cites Families (41)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4228237A (en) | 1978-09-21 | 1980-10-14 | Calbiochem-Behring Corp. | Methods for the detection and determination of ligands |
FI63596C (fi) | 1981-10-16 | 1983-07-11 | Orion Yhtymae Oy | Mikrobdiagnostiskt foerfarande som grundar sig pao skiktshybridisering av nukleinsyror och vid foerfarandet anvaenda kombinationer av reagenser |
US4994373A (en) | 1983-01-27 | 1991-02-19 | Enzo Biochem, Inc. | Method and structures employing chemically-labelled polynucleotide probes |
US5288609A (en) | 1984-04-27 | 1994-02-22 | Enzo Diagnostics, Inc. | Capture sandwich hybridization method and composition |
US4751177A (en) | 1985-06-13 | 1988-06-14 | Amgen | Methods and kits for performing nucleic acid hybridization assays |
US4868105A (en) | 1985-12-11 | 1989-09-19 | Chiron Corporation | Solution phase nucleic acid sandwich assay |
US4925785A (en) | 1986-03-07 | 1990-05-15 | Biotechnica Diagnostics, Inc. | Nucleic acid hybridization assays |
US5374524A (en) | 1988-05-10 | 1994-12-20 | E. I. Du Pont De Nemours And Company | Solution sandwich hybridization, capture and detection of amplified nucleic acids |
ATE173508T1 (de) | 1988-05-20 | 1998-12-15 | Hoffmann La Roche | Befestigung von sequenzspezifischen proben |
US6040138A (en) | 1995-09-15 | 2000-03-21 | Affymetrix, Inc. | Expression monitoring by hybridization to high density oligonucleotide arrays |
US5744101A (en) | 1989-06-07 | 1998-04-28 | Affymax Technologies N.V. | Photolabile nucleoside protecting groups |
US5547839A (en) | 1989-06-07 | 1996-08-20 | Affymax Technologies N.V. | Sequencing of surface immobilized polymers utilizing microflourescence detection |
US5143854A (en) | 1989-06-07 | 1992-09-01 | Affymax Technologies N.V. | Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof |
US5252743A (en) | 1989-11-13 | 1993-10-12 | Affymax Technologies N.V. | Spatially-addressable immobilization of anti-ligands on surfaces |
JPH05502585A (ja) * | 1989-12-04 | 1993-05-13 | ベクトン ディッキンソン アンド カンパニー | ポリマーに共有結合したオリゴヌクレオチドの利用により向上される標的核酸の捕獲 |
AU7762091A (en) | 1990-03-30 | 1991-10-30 | City Of Hope | Detection of minimal residual disease in lymphoid malignancies |
US5556748A (en) | 1991-07-30 | 1996-09-17 | Xenopore Corporation | Methods of sandwich hybridization for the quantitative analysis of oligonucleotides |
EP0534640B1 (fr) | 1991-09-23 | 1996-10-02 | Pfizer Inc. | Procédé pour détecter ARNm et ADN spécifiques dans les cellules |
US5324633A (en) | 1991-11-22 | 1994-06-28 | Affymax Technologies N.V. | Method and apparatus for measuring binding affinity |
US5985583A (en) * | 1992-06-23 | 1999-11-16 | Mount Sinai School Of Medicine Of The City University Of New York | Cloning and expression of gonadotropin-releasing hormone receptor |
US5605798A (en) | 1993-01-07 | 1997-02-25 | Sequenom, Inc. | DNA diagnostic based on mass spectrometry |
CA2162568C (fr) | 1993-05-10 | 2001-10-30 | Yuichi Oku | Methode et reactif pour la mise a l'essai d'un ligand ou simultanement de plusieurs ligands dans un groupe de ligands predetermine |
JPH079468A (ja) * | 1993-06-24 | 1995-01-13 | Sekisui Chem Co Ltd | 熱可塑性樹脂成型体の製造方法 |
DE19518217A1 (de) | 1994-05-11 | 1995-11-30 | Imtec Immundiagnostika Gmbh | Verfahren und Vorrichtung zur qualitativen und quantitativen Bestimmung von Desoxyribonukleinsäure (DNA) auf festen Phasen |
US5807522A (en) * | 1994-06-17 | 1998-09-15 | The Board Of Trustees Of The Leland Stanford Junior University | Methods for fabricating microarrays of biological samples |
ZA956776B (en) * | 1994-08-25 | 1996-03-20 | Akzo Nobel Nv | A process for making the product of a nucleic acid amplification reaction incapable of being a target for further amplification a diagnostic assay employing said process a kit and a container suitable for carrying out said process for assay |
US6974666B1 (en) | 1994-10-21 | 2005-12-13 | Appymetric, Inc. | Methods of enzymatic discrimination enhancement and surface-bound double-stranded DNA |
DE19516196A1 (de) | 1995-05-08 | 1996-11-14 | Boehringer Mannheim Gmbh | Verfahren zum quantitativen Nachweis von Nukleinsäuren |
WO1996035778A1 (fr) * | 1995-05-11 | 1996-11-14 | Human Genome Sciences, Inc. | Uridine diphosphate galactose-4-epimerase humaine |
US5545531A (en) | 1995-06-07 | 1996-08-13 | Affymax Technologies N.V. | Methods for making a device for concurrently processing multiple biological chip assays |
FR2737502B1 (fr) | 1995-07-31 | 1997-10-24 | Genset Sa | Procede de detection d'acides nucleiques utilisant des sondes nucleotidiques permettant a la fois une capture specifique et une detection |
WO1997007245A1 (fr) | 1995-08-14 | 1997-02-27 | Ely Michael Rabani | Procedes et dispositifs de sequençage multiplex parallele de polynucleotides |
US5661028A (en) | 1995-09-29 | 1997-08-26 | Lockheed Martin Energy Systems, Inc. | Large scale DNA microsequencing device |
JP2002515738A (ja) | 1996-01-23 | 2002-05-28 | アフィメトリックス,インコーポレイティド | 核酸分析法 |
CA2216227A1 (fr) * | 1996-01-24 | 1997-07-31 | Synaptic Pharmaceutical Corporation | Adn codant les recepteurs galr2 de la galanine et ses utilisations |
EP2368897B1 (fr) | 1996-02-09 | 2016-10-19 | Cornell Research Foundation, Inc. | Detection de différences entre séquences d'acide nucleique faisant appel à la réaction de détection par ligation et d'hybridation sur des réseaux adressables d'oligonucléotides |
EP0886681A1 (fr) | 1996-03-04 | 1998-12-30 | Genetrace Systems, Inc. | Methodes de criblage des acides nucleiques par spectrometrie de masse |
US5770370A (en) | 1996-06-14 | 1998-06-23 | David Sarnoff Research Center, Inc. | Nuclease protection assays |
EP0990142A4 (fr) | 1996-12-31 | 2000-09-27 | Genometrix Genomics Inc | Procede et dispositif d'analyse moleculaire multiplexee |
US6232066B1 (en) | 1997-12-19 | 2001-05-15 | Neogen, Inc. | High throughput assay system |
KR100709468B1 (ko) * | 2004-11-09 | 2007-04-18 | 주식회사 하이닉스반도체 | 플래시 메모리 소자의 플로팅 게이트 형성방법 |
-
1998
- 1998-07-02 US US09/109,076 patent/US6232066B1/en not_active Expired - Lifetime
- 1998-12-21 EP EP98964187A patent/EP1038033B1/fr not_active Expired - Lifetime
- 1998-12-21 WO PCT/US1998/027191 patent/WO1999032663A2/fr active IP Right Grant
- 1998-12-21 MX MXPA00006058A patent/MXPA00006058A/es not_active IP Right Cessation
- 1998-12-21 JP JP2000525580A patent/JP2001526904A/ja active Pending
- 1998-12-21 CN CNB988132737A patent/CN1292076C/zh not_active Expired - Lifetime
- 1998-12-21 CZ CZ20002259A patent/CZ302381B6/cs not_active IP Right Cessation
- 1998-12-21 KR KR1020007006774A patent/KR100708782B1/ko not_active IP Right Cessation
- 1998-12-21 DK DK98964187T patent/DK1038033T3/da active
- 1998-12-21 PL PL98342898A patent/PL193762B1/pl not_active IP Right Cessation
- 1998-12-21 DE DE69839373T patent/DE69839373T2/de not_active Expired - Lifetime
- 1998-12-21 AT AT98964187T patent/ATE392485T1/de active
- 1998-12-21 CA CA2315776A patent/CA2315776C/fr not_active Expired - Lifetime
- 1998-12-21 ES ES98964187T patent/ES2306484T3/es not_active Expired - Lifetime
- 1998-12-21 IL IL13686298A patent/IL136862A0/xx active IP Right Grant
- 1998-12-21 NZ NZ505319A patent/NZ505319A/xx unknown
- 1998-12-21 AU AU19366/99A patent/AU1936699A/en not_active Abandoned
- 1998-12-21 EA EA200000684A patent/EA006425B1/ru not_active IP Right Cessation
-
2000
- 2000-06-16 NO NO20003115A patent/NO329977B1/no not_active IP Right Cessation
- 2000-06-18 IL IL136862A patent/IL136862A/en not_active IP Right Cessation
-
2003
- 2003-08-12 AU AU2003234756A patent/AU2003234756A1/en not_active Abandoned
-
2009
- 2009-02-02 JP JP2009021456A patent/JP2009183286A/ja not_active Withdrawn
- 2009-10-09 JP JP2009234964A patent/JP2010046072A/ja active Pending
-
2011
- 2011-02-04 JP JP2011023036A patent/JP5049395B2/ja not_active Expired - Lifetime
-
2012
- 2012-02-03 JP JP2012022239A patent/JP2012090644A/ja not_active Withdrawn
Also Published As
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CA2315776C (fr) | Systeme de dosage a haut rendement | |
US6458533B1 (en) | High throughput assay system for monitoring ESTs | |
CA2377567C (fr) | Systeme d'analyse extremement productif | |
CA2452819C (fr) | Systeme de dosage a rendement eleve | |
US20100105572A1 (en) | High throughput assay system | |
WO2000037684A1 (fr) | Systeme d'analyse a haut rendement mettant en oeuvre la spectrometrie de masse | |
US20030039967A1 (en) | High throughput assay system using mass spectrometry | |
US20040185464A1 (en) | High throughput assay system | |
AU2007231625B2 (en) | High Throughput Assay System | |
AU2006201464B2 (en) | High Throughput Assay System |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
MM1K | Lapsed by not paying the annual fees |