NO20051049L - Celle-baserte fluorescens-resonans energi overforings (FRET)-analyser for Clostridietoksiner - Google Patents

Celle-baserte fluorescens-resonans energi overforings (FRET)-analyser for Clostridietoksiner

Info

Publication number
NO20051049L
NO20051049L NO20051049A NO20051049A NO20051049L NO 20051049 L NO20051049 L NO 20051049L NO 20051049 A NO20051049 A NO 20051049A NO 20051049 A NO20051049 A NO 20051049A NO 20051049 L NO20051049 L NO 20051049L
Authority
NO
Norway
Prior art keywords
energy transfer
cell
donor fluorophore
resonance energy
clostridietoxin
Prior art date
Application number
NO20051049A
Other languages
English (en)
Norwegian (no)
Other versions
NO20051049D0 (no
Inventor
Lance E Steward
Kei Roger Aoki
Ester Fernandes-Salas
Original Assignee
Allergan Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=32041812&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=NO20051049(L) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Allergan Inc filed Critical Allergan Inc
Publication of NO20051049D0 publication Critical patent/NO20051049D0/no
Publication of NO20051049L publication Critical patent/NO20051049L/no

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/554—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being a biological cell or cell fragment, e.g. bacteria, yeast cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04—Antibacterial agents
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/33—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Clostridium (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4702—Regulators; Modulating activity
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4725—Proteoglycans, e.g. aggreccan
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/16011—Herpesviridae
    • C12N2710/16611—Simplexvirus, e.g. human herpesvirus 1, 2
    • C12N2710/16622—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/16011—Human Immunodeficiency Virus, HIV
    • C12N2740/16111—Human Immunodeficiency Virus, HIV concerning HIV env
    • C12N2740/16122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Zoology (AREA)
  • Immunology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Toxicology (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Virology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Communicable Diseases (AREA)
  • Veterinary Medicine (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Oncology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Chemical & Material Sciences (AREA)
NO20051049A 2002-09-27 2005-02-25 Celle-baserte fluorescens-resonans energi overforings (FRET)-analyser for Clostridietoksiner NO20051049L (no)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US10/261,161 US7183066B2 (en) 2002-09-27 2002-09-27 Cell-based fluorescence resonance energy transfer (FRET) assays for clostridial toxins
PCT/US2003/028092 WO2004029576A2 (fr) 2002-09-27 2003-09-04 Tests de fluorescence de cellule par transfert d'energie de resonance (fret) concernant des toxines clostridiennes

Publications (2)

Publication Number Publication Date
NO20051049D0 NO20051049D0 (no) 2005-02-25
NO20051049L true NO20051049L (no) 2005-06-21

Family

ID=32041812

Family Applications (1)

Application Number Title Priority Date Filing Date
NO20051049A NO20051049L (no) 2002-09-27 2005-02-25 Celle-baserte fluorescens-resonans energi overforings (FRET)-analyser for Clostridietoksiner

Country Status (17)

Country Link
US (3) US7183066B2 (fr)
EP (1) EP1543329B1 (fr)
JP (1) JP4554369B2 (fr)
KR (1) KR20050071506A (fr)
CN (1) CN1296712C (fr)
AU (2) AU2003282790B2 (fr)
BR (1) BR0314795A (fr)
CA (1) CA2500040C (fr)
CO (1) CO5721032A2 (fr)
DK (1) DK1543329T3 (fr)
IL (1) IL167410A (fr)
MX (1) MXPA05003128A (fr)
NO (1) NO20051049L (fr)
PL (1) PL376103A1 (fr)
RU (1) RU2005109385A (fr)
WO (1) WO2004029576A2 (fr)
ZA (1) ZA200501500B (fr)

Families Citing this family (67)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080038274A1 (en) * 1999-09-23 2008-02-14 Foster Keith A Inhibition of secretion from non-neuronal cells
US7354735B2 (en) * 2001-05-24 2008-04-08 Hong Kong University Of Science And Technology Nucleic acid encoding fluorescent protein constructs and methods for detecting apoptosis
US20080064054A1 (en) * 2001-08-28 2008-03-13 Ester Fernandez-Salas Fluorescence resonance energy transfer (fret) assays for clostridial toxin activity
US7374896B2 (en) 2001-08-28 2008-05-20 Allergan, Inc. GFP-SNAP25 fluorescence release assay for botulinum neurotoxin protease activity
US7208285B2 (en) * 2001-08-28 2007-04-24 Allergan, Inc. Fret protease assays for botulinum serotype A/E toxins
US7332567B2 (en) * 2001-08-28 2008-02-19 Allergan, Inc. Fret protease assays for clostridial toxins
US20060063221A1 (en) * 2004-09-21 2006-03-23 Williams Dudley J Lanthanide-based substrates and methods for determining clostridial toxin activity
US8022172B2 (en) * 2001-08-28 2011-09-20 Allergan, Inc. Luminescence resonance energy transfer (LRET) assays for clostridial toxin activity
US7183066B2 (en) * 2002-09-27 2007-02-27 Allergan, Inc. Cell-based fluorescence resonance energy transfer (FRET) assays for clostridial toxins
AU2003272800A1 (en) * 2002-10-01 2004-04-23 University Of Maryland Methods for identifying inhibitors of botulinum neurotoxins
JP5178009B2 (ja) * 2003-12-19 2013-04-10 ウィスコンシン アルムニ リサーチ ファンデイション ボツリヌス神経毒素検出のための方法および複合体
US8497081B2 (en) * 2004-02-24 2013-07-30 Allergan, Inc. Botulinum toxin screening assays
DK1718756T3 (da) * 2004-02-24 2012-02-27 Allergan Inc Botulinumtoksinscreeningassays
EP1784420B1 (fr) * 2004-09-01 2008-12-03 Allergan, Inc. Toxines clostridiales degradables
US8617838B2 (en) * 2004-09-20 2013-12-31 University Of Massachusetts Fluorescent proteins and related methods and compounds
US7399607B2 (en) * 2004-09-22 2008-07-15 Allergan, Inc. Fluorescence polarization assays for determining clostridial toxin activity
US8031338B2 (en) * 2004-12-02 2011-10-04 Vanderbilt University Measuring Forster resonance energy transfer with polarized and depolarized light
EP2264458A1 (fr) 2005-04-05 2010-12-22 Allergan, Inc. Analyses de l'activité de la toxine clostridienne
CA2604039C (fr) * 2005-04-05 2014-09-16 Allergan, Inc. Analyses fret a base de colorants lipophiles a la recherche d'une activite de la toxine clostridiale
DE102005020384A1 (de) * 2005-05-02 2006-11-09 Therainvention Gmbh Spektroskopisches Verfahren zum Nachweis von Analyten
DK2154151T3 (da) * 2005-09-19 2011-09-05 Allergan Inc Clostridiumtoksinaktiverbare clostridiumtoksiner
US7998749B2 (en) * 2005-10-12 2011-08-16 Allergan, Inc. Assays of molecular or subcellular interactivity using depolarization after resonance transfer energy (DARET)
FR2903495A1 (fr) * 2006-07-06 2008-01-11 Pharmaleads Soc Par Actions Si PROCEDE DE DETECTION D'INTERACTIONS A L'AIDE DU RAPPORTEUR DE FLUORESCENCE Pya
JP2008220302A (ja) * 2007-03-14 2008-09-25 Hiroshima Univ タンパク質蓄積による神経変性疾患治療薬物のスクリーニング系
US7733470B2 (en) * 2007-06-26 2010-06-08 Photon Technology International, Inc. Method and apparatus for real-time measurement and calculation of a fluorescent lifetime
CN105907718B (zh) 2007-09-14 2020-10-27 拜奥麦迪逊公司 利用切割序列和间隔子的共振能量转移测定
KR101604515B1 (ko) * 2008-03-14 2016-03-17 알러간, 인코포레이티드 면역-기반 보툴리눔 독소 세로타입 ａ 활성 검정
EP3556770A1 (fr) 2008-03-14 2019-10-23 Allergan, Inc. Essais d'activité de toxine botulique de sérotype a
CN105833254A (zh) 2008-12-10 2016-08-10 阿勒根公司 梭菌毒素药物组合物
US8492109B2 (en) * 2009-01-20 2013-07-23 Trustees Of Tufts College Methods for the delivery of toxins or enzymatically active portions thereof
JP5717657B2 (ja) 2009-03-13 2015-05-13 アラーガン、インコーポレイテッドＡｌｌｅｒｇａｎ，Ｉｎｃｏｒｐｏｒａｔｅｄ 免疫系ボツリヌス毒素血清型ａ活性アッセイに有用な細胞
AU2010223912B2 (en) 2009-03-13 2015-09-24 Allergan, Inc. Immuno-based retargeted endopeptidase activity assays
US9247611B2 (en) * 2009-06-01 2016-01-26 Apple Inc. Light source with light sensor
US8129139B2 (en) 2009-07-13 2012-03-06 Allergan, Inc. Process for obtaining botulinum neurotoxin
WO2011039953A1 (fr) * 2009-09-29 2011-04-07 三井造船株式会社 Procédé et dispositif pour la mesure d'un fret
WO2011041779A1 (fr) 2009-10-02 2011-04-07 Allergan, Inc. Procédé pour déterminer des changements in vitro dans un environnement protéique
FR2958293B1 (fr) 2010-04-01 2014-09-26 Centre Nat Rech Scient Outils pour l'identification de ligands de lingo-1,lingo-2, lingo-3 et lingo-4, et utilisations
CN102243176B (zh) * 2010-05-14 2013-11-20 中国人民解放军军事医学科学院毒物药物研究所 一种体外分子水平荧光法排除筛选药物假阳性结果的方法
WO2012047325A2 (fr) * 2010-06-11 2012-04-12 Synaptic Research, Llc Procédés d'indication d'activité de protéase de règle n-terminale
EP2623514B1 (fr) * 2010-09-27 2017-07-26 Kyoto University Pont pour biocapteur fret unimoléculaire basé sur le principe du transfert d'énergie par résonance de fluorescence
US8569254B2 (en) 2010-12-10 2013-10-29 National Yang Ming University Methods for modulating the expression and aggregation of CAG-expanded gene product in cells and methods for identifying agents useful for doing the same
US9303285B2 (en) 2012-01-04 2016-04-05 Biomadison, Inc. Methods and compounds for increasing sensitivity of botulinum assays
US10908146B2 (en) 2011-06-01 2021-02-02 Biomadison, Inc. Compositions and methods for improving sensitivity in cell based assays
US11325954B2 (en) 2011-06-01 2022-05-10 Biomadison, Inc. Compositions and methods for stability testing of botulinum toxin
CN107475293A (zh) * 2011-06-01 2017-12-15 拜奥麦迪逊公司 非‑fret肉毒梭菌测定法
US10451621B2 (en) 2011-12-31 2019-10-22 Allergan, Inc. Highly sensitive cell-based assay to detect the presence of active botulinum neurotoxin serotype-A
WO2013103983A1 (fr) * 2012-01-06 2013-07-11 The Research Foundation Of State University Of New York Criblage à haut débit de cellules vivantes
GB201219024D0 (en) * 2012-10-23 2012-12-05 Syntaxin Ltd Assay
EP3270162B2 (fr) 2013-08-09 2023-08-16 BioMadison, Inc. Botulinumtoxin-assay mit erhöhter empfindlichkeit
US20150118701A1 (en) 2013-10-25 2015-04-30 Biomadison, Inc. Enzyme assay with duplicate fluorophores
KR20170026624A (ko) 2014-07-07 2017-03-08 알러간, 인코포레이티드 조직 샘플에서 절단된 snap25를 검출하는 방법
AU2015301737B2 (en) * 2014-08-12 2021-01-28 Biomadison, Inc. Botulinum neurotoxins with modified light chain specifity and methods for producing same
CN104359883A (zh) * 2014-11-13 2015-02-18 贵阳医学院 人源沉默信息调节因子6的活性荧光检测方法
KR102557798B1 (ko) 2014-12-23 2023-07-19 메르츠 파마 게엠베하 운트 코. 카가아 보툴리눔 독소 사전충전된 용기
CN105784656B (zh) * 2016-03-16 2019-01-01 大连理工大学 一种检测活细胞内RhoGDIα蛋白活性的生物探针
TWI737742B (zh) 2016-06-22 2021-09-01 德商梅茲製藥有限兩合公司 肉毒桿菌毒素的預填充式注射器系統、具有其之套組以及其之使用
RU2019110875A (ru) 2016-09-13 2020-10-15 Аллерган, Инк. Небелковые композиции клостридиального токсина
US11129906B1 (en) 2016-12-07 2021-09-28 David Gordon Bermudes Chimeric protein toxins for expression by therapeutic bacteria
CN108305912B (zh) * 2017-01-11 2024-03-26 中国科学院上海微系统与信息技术研究所 具有波长选择性的石墨烯仿生光探测器及其制备方法
EP3635126B1 (fr) * 2017-06-07 2021-07-07 Original G B.V. Procédé de détection de microbes altérant les aliments
JP7316266B2 (ja) * 2018-03-09 2023-07-27 ザルトリウス バイオアナリティカル インストゥルメンツ， インコーポレイテッド 細胞内ａｔｐの生細胞分析のための方法と組成物
CN108333342A (zh) * 2018-05-14 2018-07-27 广东药科大学 一种快速检测牛奶中四环素类药物残留的方法
BR112021005774A2 (pt) * 2018-09-28 2021-07-06 Inst Of Molecular And Cell Biology Biomedical Sciences Insts ensaios de neurotoxina de clostridiana à base de célula
CN109211863B (zh) * 2018-10-26 2021-04-20 大连民族大学 利用Eu2+f-f跃迁光谱检测爆炸物TNP的方法
TW202113072A (zh) * 2019-06-07 2021-04-01 美商辛那皮克研究有限責任公司 基因工程化細胞
US20210373021A1 (en) * 2020-06-01 2021-12-02 Galderma Holding S.A. Snap-25 reporter constructs and methods of using the same
CN112557353B (zh) * 2020-12-17 2022-02-18 天津工业大学 一种基于延迟发光光谱的细胞活力检测方法和装置

Family Cites Families (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH05505102A (ja) * 1989-12-21 1993-08-05 ホワイトヘツド・インスチチユート・フオー・バイオメデイカル・リサーチ 真核細胞の中に分子を配達する方法
US5804604A (en) * 1989-12-21 1998-09-08 Biogen, Inc. Tat-derived transport polypeptides and fusion proteins
US5962637A (en) * 1994-06-03 1999-10-05 Microbiological Research Authority Toxin assay
GB9411138D0 (en) 1994-06-03 1994-07-27 Microbiological Res Authority Toxin assay
GB9508204D0 (en) * 1995-04-21 1995-06-07 Speywood Lab Ltd A novel agent able to modify peripheral afferent function
US6069010A (en) 1995-09-11 2000-05-30 Axys Pharmaceuticals, Inc. High throughput gene inactivation with large scale gene targeting
US6803188B1 (en) * 1996-01-31 2004-10-12 The Regents Of The University Of California Tandem fluorescent protein constructs
US5965699A (en) * 1996-11-06 1999-10-12 The United States Of America As Represented By The Secretary Of The Army Assay for the proteolytic activity of serotype a from clostridium botulinum
AU1818299A (en) 1997-12-10 1999-06-28 Washington University Anti-pathogen system and methods of use thereof
CA2330560A1 (fr) 1998-04-28 1999-11-04 Washington University Methodes de transduction de molecules de fusion
US7090976B2 (en) * 1999-11-10 2006-08-15 Rigel Pharmaceuticals, Inc. Methods and compositions comprising Renilla GFP
WO2000026408A2 (fr) 1998-10-30 2000-05-11 Cellomics, Inc. Systeme de criblage pour cellules
EP1137664A2 (fr) 1998-12-10 2001-10-04 Washington University Systeme de transduction de proteines et methodes d'utilisation
DE19856897A1 (de) * 1998-12-10 2000-06-15 Biotecon Ges Fuer Biotechnologische Entwicklung & Consulting Mbh Therapeutikum zur Unterdrückung von Schnarchgeräuschen
US7410769B2 (en) 2000-02-11 2008-08-12 Cellomics Inc. Peptide biosensors for anthrax protease
US6325899B1 (en) * 2000-03-10 2001-12-04 Action Caps, Llc Disposable and recyclable intermediates for use in electrostatic coating processes
FR2809733B1 (fr) * 2000-06-02 2004-04-30 Inst Nat Sante Rech Med Substrat peptidique reconnu par la toxine botulique de type bont/b et son utilisation pour doser et/ou detecter ladite toxine ou des inhibiteurs correspondants
WO2002025284A2 (fr) 2000-09-25 2002-03-28 U.S. Medical Research Institute Of Infectious Diseases Tests de detection rapide des activites proteolytiques des neurotoxines clostridiales
DE60117180T2 (de) * 2000-11-22 2006-08-10 The Regents Of The University Of California, Oakland Verfahren zur messung des aktivierungszustands von signalwegen in zellen
US20030104975A1 (en) * 2001-06-14 2003-06-05 Johan Auwerx Cofactor-based screening method for nuclear receptor modulators and related modulators
US7374896B2 (en) * 2001-08-28 2008-05-20 Allergan, Inc. GFP-SNAP25 fluorescence release assay for botulinum neurotoxin protease activity
US20080064054A1 (en) * 2001-08-28 2008-03-13 Ester Fernandez-Salas Fluorescence resonance energy transfer (fret) assays for clostridial toxin activity
US8022172B2 (en) * 2001-08-28 2011-09-20 Allergan, Inc. Luminescence resonance energy transfer (LRET) assays for clostridial toxin activity
US7208285B2 (en) * 2001-08-28 2007-04-24 Allergan, Inc. Fret protease assays for botulinum serotype A/E toxins
US7332567B2 (en) * 2001-08-28 2008-02-19 Allergan, Inc. Fret protease assays for clostridial toxins
US7183066B2 (en) * 2002-09-27 2007-02-27 Allergan, Inc. Cell-based fluorescence resonance energy transfer (FRET) assays for clostridial toxins
DK1718756T3 (da) * 2004-02-24 2012-02-27 Allergan Inc Botulinumtoksinscreeningassays
US7399607B2 (en) * 2004-09-22 2008-07-15 Allergan, Inc. Fluorescence polarization assays for determining clostridial toxin activity
CA2604039C (fr) * 2005-04-05 2014-09-16 Allergan, Inc. Analyses fret a base de colorants lipophiles a la recherche d'une activite de la toxine clostridiale
US7998749B2 (en) * 2005-10-12 2011-08-16 Allergan, Inc. Assays of molecular or subcellular interactivity using depolarization after resonance transfer energy (DARET)

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MXPA05003128A (es) 2005-06-22
CN1685234A (zh) 2005-10-19
US20040072270A1 (en) 2004-04-15
US7183066B2 (en) 2007-02-27
JP2006502378A (ja) 2006-01-19
AU2009222589A1 (en) 2009-10-29
CA2500040C (fr) 2014-12-23
EP1543329B1 (fr) 2016-08-03
NO20051049D0 (no) 2005-02-25
PL376103A1 (en) 2005-12-12
AU2009222589B2 (en) 2011-12-22
WO2004029576A2 (fr) 2004-04-08
EP1543329A2 (fr) 2005-06-22
KR20050071506A (ko) 2005-07-07
US7749759B2 (en) 2010-07-06
CO5721032A2 (es) 2007-01-31
US20070122858A1 (en) 2007-05-31
JP4554369B2 (ja) 2010-09-29
IL167410A (en) 2011-12-29
ZA200501500B (en) 2005-10-26
EP1543329A4 (fr) 2006-10-18
AU2003282790B2 (en) 2009-07-02
RU2005109385A (ru) 2006-01-27
AU2003282790A1 (en) 2004-04-19
BR0314795A (pt) 2005-07-26
DK1543329T3 (en) 2016-12-05
US20090117572A1 (en) 2009-05-07
CA2500040A1 (fr) 2004-04-08
CN1296712C (zh) 2007-01-24

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