ATE540317T1 - Fret-protease-assays für clostridielle toxine - Google Patents
Fret-protease-assays für clostridielle toxineInfo
- Publication number
- ATE540317T1 ATE540317T1 AT07023843T AT07023843T ATE540317T1 AT E540317 T1 ATE540317 T1 AT E540317T1 AT 07023843 T AT07023843 T AT 07023843T AT 07023843 T AT07023843 T AT 07023843T AT E540317 T1 ATE540317 T1 AT E540317T1
- Authority
- AT
- Austria
- Prior art keywords
- clostridial toxin
- donor fluorophore
- acceptor
- lanthanide donor
- clostridial toxins
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
- C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/001—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof by chemical synthesis
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56911—Bacteria
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
- G01N2333/33—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Clostridium (G)
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Immunology (AREA)
- Biochemistry (AREA)
- Zoology (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- Genetics & Genomics (AREA)
- Hematology (AREA)
- Microbiology (AREA)
- Urology & Nephrology (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biomedical Technology (AREA)
- Wood Science & Technology (AREA)
- Gastroenterology & Hepatology (AREA)
- Virology (AREA)
- Food Science & Technology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Cell Biology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Tropical Medicine & Parasitology (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Toxicology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Enzymes And Modification Thereof (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US09/942,098 US7332567B2 (en) | 2001-08-28 | 2001-08-28 | Fret protease assays for clostridial toxins |
Publications (1)
Publication Number | Publication Date |
---|---|
ATE540317T1 true ATE540317T1 (de) | 2012-01-15 |
Family
ID=27613894
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AT07023843T ATE540317T1 (de) | 2001-08-28 | 2002-08-22 | Fret-protease-assays für clostridielle toxine |
AT02807745T ATE541213T1 (de) | 2001-08-28 | 2002-08-22 | Fret-protease-assays für clostridientoxine |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AT02807745T ATE541213T1 (de) | 2001-08-28 | 2002-08-22 | Fret-protease-assays für clostridientoxine |
Country Status (9)
Country | Link |
---|---|
US (10) | US7332567B2 (de) |
EP (2) | EP1438586B1 (de) |
JP (3) | JP2005520000A (de) |
AT (2) | ATE540317T1 (de) |
AU (1) | AU2002368109B8 (de) |
BR (1) | BRPI0212140B8 (de) |
CA (1) | CA2462686C (de) |
DK (2) | DK1901069T3 (de) |
WO (1) | WO2004031773A1 (de) |
Families Citing this family (52)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20060141572A1 (en) * | 1993-09-21 | 2006-06-29 | Smith Leonard A | Recombinant light chains of botulinum neurotoxins and light chain fusion proteins for use in research and clinical therapy |
FR2809733B1 (fr) * | 2000-06-02 | 2004-04-30 | Inst Nat Sante Rech Med | Substrat peptidique reconnu par la toxine botulique de type bont/b et son utilisation pour doser et/ou detecter ladite toxine ou des inhibiteurs correspondants |
US7354735B2 (en) * | 2001-05-24 | 2008-04-08 | Hong Kong University Of Science And Technology | Nucleic acid encoding fluorescent protein constructs and methods for detecting apoptosis |
US20080064054A1 (en) * | 2001-08-28 | 2008-03-13 | Ester Fernandez-Salas | Fluorescence resonance energy transfer (fret) assays for clostridial toxin activity |
US7332567B2 (en) * | 2001-08-28 | 2008-02-19 | Allergan, Inc. | Fret protease assays for clostridial toxins |
US7208285B2 (en) * | 2001-08-28 | 2007-04-24 | Allergan, Inc. | Fret protease assays for botulinum serotype A/E toxins |
US8022172B2 (en) * | 2001-08-28 | 2011-09-20 | Allergan, Inc. | Luminescence resonance energy transfer (LRET) assays for clostridial toxin activity |
US20060063221A1 (en) * | 2004-09-21 | 2006-03-23 | Williams Dudley J | Lanthanide-based substrates and methods for determining clostridial toxin activity |
US7374896B2 (en) * | 2001-08-28 | 2008-05-20 | Allergan, Inc. | GFP-SNAP25 fluorescence release assay for botulinum neurotoxin protease activity |
US7183066B2 (en) * | 2002-09-27 | 2007-02-27 | Allergan, Inc. | Cell-based fluorescence resonance energy transfer (FRET) assays for clostridial toxins |
AU2003272800A1 (en) * | 2002-10-01 | 2004-04-23 | University Of Maryland | Methods for identifying inhibitors of botulinum neurotoxins |
US20040115727A1 (en) * | 2002-12-11 | 2004-06-17 | Allergan, Inc., A Corporation | Evolved clostridial toxins with altered protease specificity |
CA2550401C (en) | 2003-12-19 | 2015-12-08 | Wisconsin Alumni Research Foundation | Method and compositions for detecting botulinum neurotoxin |
US20080274480A1 (en) * | 2004-08-04 | 2008-11-06 | Allergan, Inc. | Botulinum Toxin Type a Immunoresistant Assay |
WO2006101520A2 (en) * | 2004-09-20 | 2006-09-28 | University Of Massachusetts | Fluorescent proteins and related methods and compounds |
US7399607B2 (en) * | 2004-09-22 | 2008-07-15 | Allergan, Inc. | Fluorescence polarization assays for determining clostridial toxin activity |
EP1824971B1 (de) | 2004-11-22 | 2016-01-13 | New York University | Genetisch veränderte clostridiengene, durch die veränderten gene kodierte proteine und deren verwendungen |
WO2008036060A2 (en) | 2005-04-05 | 2008-03-27 | Allergan, Inc. | Clostridial toxin activity assays |
PT1869459E (pt) * | 2005-04-05 | 2010-08-10 | Allergan Inc | Ensaios fret à base de corantes lipofílicos para a actividade da toxina de clostrídeo |
DE602006013481D1 (de) | 2005-09-19 | 2010-05-20 | Allergan Inc | Mit clostriedientoxin aktivierbare clostridientoxine |
CA2602972C (en) * | 2005-10-12 | 2013-09-24 | Allergan, Inc. | Assays of molecular or subcellular interactivity using depolarization after resonance energy transfer (daret) |
US8753831B2 (en) * | 2007-06-05 | 2014-06-17 | City Of Hope | Methods for detection of botulinum neurotoxin |
CN101932936B (zh) | 2007-09-14 | 2016-04-27 | 拜奥麦迪逊公司 | 利用切割序列和间隔子的共振能量转移测定 |
KR101604515B1 (ko) | 2008-03-14 | 2016-03-17 | 알러간, 인코포레이티드 | 면역-기반 보툴리눔 독소 세로타입 a 활성 검정 |
KR101609894B1 (ko) | 2008-03-14 | 2016-04-08 | 알러간, 인코포레이티드 | 면역-기반 보툴리눔 독소 세로타입 a 활성 검정 |
RU2535003C2 (ru) | 2008-12-10 | 2014-12-10 | Аллерган, Инк. | Фармацевтические композиции токсина клостридий |
RU2543650C2 (ru) | 2009-03-13 | 2015-03-10 | Аллерган, Инк. | Иммунологические тесты на активность эндопептидаз с измененной нацеленностью |
KR20200146046A (ko) | 2009-03-13 | 2020-12-31 | 알러간, 인코포레이티드 | 면역 기반 보툴리눔 독소 혈청형 a 활성 검정에 유용한 세포 |
WO2010124998A1 (en) * | 2009-04-27 | 2010-11-04 | Merz Pharma Gmbh & Co. Kgaa | Means and methods for the determination of the amount of neurotoxin polypeptide and of its catalytic and proteolytic activities |
US8129139B2 (en) | 2009-07-13 | 2012-03-06 | Allergan, Inc. | Process for obtaining botulinum neurotoxin |
EP2483666A1 (de) | 2009-10-02 | 2012-08-08 | Allergan, Inc. | In-vitro-verfahren zur bestimmung der änderungen in einer proteinumgebung |
US9453254B2 (en) * | 2009-10-16 | 2016-09-27 | Biomadison, Inc. | Resonance energy transfer assay with synaptobrevin substrate moiety |
WO2011091419A2 (en) * | 2010-01-25 | 2011-07-28 | New York Universiy | Recombinant derivatives of botulinum neurotoxins engineered for trafficking studies and neuronal delivery |
US8569254B2 (en) | 2010-12-10 | 2013-10-29 | National Yang Ming University | Methods for modulating the expression and aggregation of CAG-expanded gene product in cells and methods for identifying agents useful for doing the same |
US9217172B2 (en) | 2011-09-29 | 2015-12-22 | Cellsnap, Llc | Compositions and methods for toxigenicity testing |
WO2013102088A2 (en) | 2011-12-31 | 2013-07-04 | Allergan, Inc. | Highly Sensitive Cell-Based Assay to Detect the Presence of Active Botulinum Neurotoxin Serotype-A |
US8962340B2 (en) * | 2012-12-03 | 2015-02-24 | Src, Inc. | Real-time assay for the detection of botulinum toxin |
GB2512691B (en) * | 2012-12-03 | 2016-06-29 | Src Inc | Real-time assay for the detection of botulinum toxin |
EP2943585B1 (de) * | 2013-01-09 | 2018-07-11 | Friedrich-Alexander-Universität Erlangen-Nürnberg | Verfahren für in-vitro-nachweis und überwachung einer erkrankung durch messung der erkrankungsassoziierten proteaseaktivität in extrazellulären vesikeln |
DK2948174T3 (da) | 2013-01-28 | 2019-12-02 | Univ New York | Fremgangsmåder til behandling under anvendelse af atoksiske neurotoksinderivater |
RU2704808C2 (ru) | 2013-06-28 | 2019-10-31 | Мерц Фарма Гмбх Энд Ко. Кгаа | Средства и способы для определения биологической активности полипептидов нейротоксина в клетках |
KR20170026624A (ko) | 2014-07-07 | 2017-03-08 | 알러간, 인코포레이티드 | 조직 샘플에서 절단된 snap25를 검출하는 방법 |
PT3177640T (pt) | 2014-08-08 | 2020-08-31 | Univ Leland Stanford Junior | Agentes pd-1 de alta afinidade e métodos de utilização |
CN107109389A (zh) * | 2014-08-12 | 2017-08-29 | 拜奥麦迪逊公司 | 具有修饰的轻链特异性的肉毒杆菌神经毒素及其生产方法 |
CA2969463A1 (en) | 2014-12-09 | 2016-06-16 | New York University | Clostridial neurotoxin fusion proteins, propeptide fusions, their expression, and use |
CN106117525B (zh) * | 2016-06-27 | 2018-01-05 | 温州医科大学 | pH敏感嵌段聚合物、FRET复合物及其制备方法 |
AU2017327369A1 (en) | 2016-09-13 | 2019-05-02 | Allergan, Inc. | Stabilized non-protein Clostridial toxin compositions |
WO2018073370A1 (en) * | 2016-10-21 | 2018-04-26 | Toxogen Gmbh | A functional detection assay devoid of antibodies for serotyping of botulinum neurotoxins |
US11129906B1 (en) | 2016-12-07 | 2021-09-28 | David Gordon Bermudes | Chimeric protein toxins for expression by therapeutic bacteria |
US11499965B2 (en) | 2017-02-08 | 2022-11-15 | Tokyo Institute Of Technology | Kit for detecting antigen or measuring its amount |
GB201702500D0 (en) * | 2017-02-16 | 2017-04-05 | Univ Sheffield | Stable vamp reporter assay |
CN109211863B (zh) * | 2018-10-26 | 2021-04-20 | 大连民族大学 | 利用Eu2+f-f跃迁光谱检测爆炸物TNP的方法 |
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US6197928B1 (en) * | 1997-03-14 | 2001-03-06 | The Regents Of The University Of California | Fluorescent protein sensors for detection of analytes |
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US6221355B1 (en) * | 1997-12-10 | 2001-04-24 | Washington University | Anti-pathogen system and methods of use thereof |
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DE69941085D1 (de) * | 1998-07-17 | 2009-08-20 | Univ Maryland | Manipulierte proteine zum analyten nachweis |
WO2000034308A2 (en) | 1998-12-10 | 2000-06-15 | Washington University | Protein transduction system and methods of use thereof |
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US6469154B1 (en) * | 1999-05-21 | 2002-10-22 | The Regents Of The University Of California | Fluorescent protein indicators |
CA2379532A1 (en) * | 1999-08-20 | 2001-03-15 | Imperial College Innovations Limited | Isoforms of snare molecules and the uses thereof in modulation of cellular exocytosis |
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US7491799B2 (en) * | 2000-07-21 | 2009-02-17 | Allergan, Inc. | Modified botulinum neurotoxins |
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US20030219462A1 (en) * | 2000-07-21 | 2003-11-27 | Allergan Sales, Inc | Clostridial neurotoxin compositions and modified clostridial neurotoxins |
CA2451909C (en) | 2000-09-25 | 2010-12-21 | James J. Schmidt | High throughput assays for the proteolytic activities of clostridial neurotoxins |
US7273722B2 (en) * | 2000-11-29 | 2007-09-25 | Allergan, Inc. | Neurotoxins with enhanced target specificity |
JP4686906B2 (ja) * | 2001-06-04 | 2011-05-25 | 株式会社日立製作所 | 波長多重用光伝送装置 |
US7332567B2 (en) * | 2001-08-28 | 2008-02-19 | Allergan, Inc. | Fret protease assays for clostridial toxins |
US20080064054A1 (en) * | 2001-08-28 | 2008-03-13 | Ester Fernandez-Salas | Fluorescence resonance energy transfer (fret) assays for clostridial toxin activity |
US20060063221A1 (en) * | 2004-09-21 | 2006-03-23 | Williams Dudley J | Lanthanide-based substrates and methods for determining clostridial toxin activity |
US8022172B2 (en) * | 2001-08-28 | 2011-09-20 | Allergan, Inc. | Luminescence resonance energy transfer (LRET) assays for clostridial toxin activity |
US7374896B2 (en) * | 2001-08-28 | 2008-05-20 | Allergan, Inc. | GFP-SNAP25 fluorescence release assay for botulinum neurotoxin protease activity |
US7208285B2 (en) * | 2001-08-28 | 2007-04-24 | Allergan, Inc. | Fret protease assays for botulinum serotype A/E toxins |
US6504006B1 (en) * | 2001-10-12 | 2003-01-07 | Nancy Rose Shine | Substrate peptides and assays for detecting and measuring proteolytic activity of serotype A neurotoxin from clostridium botulinum |
US7183066B2 (en) | 2002-09-27 | 2007-02-27 | Allergan, Inc. | Cell-based fluorescence resonance energy transfer (FRET) assays for clostridial toxins |
AU2003272800A1 (en) | 2002-10-01 | 2004-04-23 | University Of Maryland | Methods for identifying inhibitors of botulinum neurotoxins |
US20040115727A1 (en) * | 2002-12-11 | 2004-06-17 | Allergan, Inc., A Corporation | Evolved clostridial toxins with altered protease specificity |
US7611856B2 (en) * | 2003-11-05 | 2009-11-03 | Los Alamos National Security, Llc | Mass spectrometry-based methods for detection and differentiation of botulinum neurotoxins |
CA2550401C (en) * | 2003-12-19 | 2015-12-08 | Wisconsin Alumni Research Foundation | Method and compositions for detecting botulinum neurotoxin |
ATE533853T1 (de) * | 2004-02-24 | 2011-12-15 | Allergan Inc | Botulinumtoxin-screening-assays |
US7514088B2 (en) * | 2005-03-15 | 2009-04-07 | Allergan, Inc. | Multivalent Clostridial toxin derivatives and methods of their use |
US7811584B2 (en) * | 2004-06-30 | 2010-10-12 | Allergan, Inc. | Multivalent clostridial toxins |
US20060024331A1 (en) * | 2004-08-02 | 2006-02-02 | Ester Fernandez-Salas | Toxin compounds with enhanced membrane translocation characteristics |
US7892565B2 (en) * | 2004-09-01 | 2011-02-22 | Allergan, Inc. | Degradable clostridial toxins |
US7399607B2 (en) * | 2004-09-22 | 2008-07-15 | Allergan, Inc. | Fluorescence polarization assays for determining clostridial toxin activity |
PT1869459E (pt) * | 2005-04-05 | 2010-08-10 | Allergan Inc | Ensaios fret à base de corantes lipofílicos para a actividade da toxina de clostrídeo |
WO2008036060A2 (en) * | 2005-04-05 | 2008-03-27 | Allergan, Inc. | Clostridial toxin activity assays |
DE602006013481D1 (de) * | 2005-09-19 | 2010-05-20 | Allergan Inc | Mit clostriedientoxin aktivierbare clostridientoxine |
CA2602972C (en) * | 2005-10-12 | 2013-09-24 | Allergan, Inc. | Assays of molecular or subcellular interactivity using depolarization after resonance energy transfer (daret) |
US8273865B2 (en) * | 2006-03-15 | 2012-09-25 | Allergan, Inc. | Multivalent clostridial toxins |
US7691587B2 (en) * | 2006-06-01 | 2010-04-06 | Allergan, Inc. | Determining and reducing immunoresistance to a botulinum toxin therapy using botulinum toxin B peptides |
EP2038298A2 (de) * | 2006-07-11 | 2009-03-25 | Allergan, Inc. | Modifizierte clostridientoxine mit verbesserten translokationsfähigkeiten und geänderter targeting-aktivität für clostridientoxin-targetzellen |
US8067192B2 (en) * | 2007-06-05 | 2011-11-29 | City Of Hope | Methods for detection of botulinum neurotoxin |
AU2010353292A1 (en) * | 2009-12-16 | 2012-07-12 | Allergan, Inc. | Modified Clostridial toxins comprising an integrated protease cleavage site-binding domain |
-
2001
- 2001-08-28 US US09/942,098 patent/US7332567B2/en not_active Expired - Lifetime
-
2002
- 2002-08-22 DK DK07023843.1T patent/DK1901069T3/da active
- 2002-08-22 AU AU2002368109A patent/AU2002368109B8/en not_active Expired
- 2002-08-22 EP EP02807745A patent/EP1438586B1/de not_active Revoked
- 2002-08-22 AT AT07023843T patent/ATE540317T1/de active
- 2002-08-22 WO PCT/US2002/027212 patent/WO2004031773A1/en active Application Filing
- 2002-08-22 DK DK02807745.1T patent/DK1438586T3/da active
- 2002-08-22 BR BRPI0212140A patent/BRPI0212140B8/pt not_active IP Right Cessation
- 2002-08-22 JP JP2004541415A patent/JP2005520000A/ja active Pending
- 2002-08-22 EP EP07023843A patent/EP1901069B1/de not_active Revoked
- 2002-08-22 CA CA2462686A patent/CA2462686C/en not_active Expired - Lifetime
- 2002-08-22 AT AT02807745T patent/ATE541213T1/de active
-
2007
- 2007-07-20 US US11/780,925 patent/US20080038756A1/en not_active Abandoned
- 2007-07-20 US US11/780,872 patent/US20080032318A1/en not_active Abandoned
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2008
- 2008-03-12 JP JP2008062974A patent/JP2008174567A/ja active Pending
- 2008-03-12 JP JP2008062977A patent/JP2008201786A/ja active Pending
- 2008-10-21 US US12/192,798 patent/US20090042231A1/en not_active Abandoned
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2009
- 2009-11-17 US US12/620,417 patent/US8053209B2/en not_active Expired - Fee Related
- 2009-11-17 US US12/620,370 patent/US8003753B2/en not_active Expired - Fee Related
- 2009-11-17 US US12/620,388 patent/US8048643B2/en not_active Expired - Fee Related
- 2009-11-17 US US12/620,337 patent/US20100075346A1/en not_active Abandoned
- 2009-11-17 US US12/620,434 patent/US8013113B2/en not_active Expired - Fee Related
- 2009-11-17 US US12/620,400 patent/US8053208B2/en not_active Expired - Fee Related
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