ES2351942T3 - Analisis de la actividad de una toxina clostridial. - Google Patents
Analisis de la actividad de una toxina clostridial. Download PDFInfo
- Publication number
- ES2351942T3 ES2351942T3 ES06851224T ES06851224T ES2351942T3 ES 2351942 T3 ES2351942 T3 ES 2351942T3 ES 06851224 T ES06851224 T ES 06851224T ES 06851224 T ES06851224 T ES 06851224T ES 2351942 T3 ES2351942 T3 ES 2351942T3
- Authority
- ES
- Spain
- Prior art keywords
- clostridial toxin
- activity
- analysis
- cleavage site
- fluorescent member
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56911—Bacteria
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
- G01N2333/33—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Clostridium (G)
Abstract
Uso de una composición que comprende una población de células, población que comprende células que contienen un sustrato de toxina clostridial exógeno que puede ser localizado en la membrana plasmática de dichas células; en el que dichas células son capaces de permitir una intoxicación por una toxina clostridial posibilitando el mecanismo celular global mediante el cual una toxina clostridial escinde proteolíticamente un sustrato, y engloba la unión de una toxina clostridial con un receptor de baja o alta afinidad, la interiorización del complejo de toxina/receptor, la translocación de la cadena ligera de la toxina clostridial en el citoplasma y la modificación de la diana enzimática de un sustrato toxina clostridial; en el que las células capaces de permitir una intoxicación por una toxina clostridial son células que expresan uno o más receptores de toxina clostridial de baja o de alta afinidad endógenos para uno o más serotipos de toxina clostridial; expresan uno o más receptores de toxina clostridial de baja o de alta afinidad exógenos para uno o más serotipos de toxina clostridial; o expresan una combinación de receptores de toxina clostridial de baja o alta afinidad endógenos y receptores de toxina clostridial de baja o alta afinidad exógenos para uno o más serotipos de toxina clostridial; en el que dicho sustrato de toxina clostridial exógeno consiste en un miembro fluorescente, en el que dicho miembro fluorescente es un péptido que bien puede absorber inherentemente energía luminosa de una cierta longitud de onda y emitir energía luminosa de una longitud de onda diferente o que forma parte de un complejo que absorbe energía luminosa de una cierta longitud de onda y emite energía luminosa de una longitud de onda diferente; un dominio de dirección a la membrana que dirige el sustrato a la membrana celular; y una secuencia de reconocimiento de toxina clostridial que comprende un sitio de escisión que es un enlace escindible junto con elementos de reconocimiento para la proteolisis detectable en el enlace escindible por parte de una toxina clostridial, en la que el sitio de escisión se encuentra entre dicho miembro fluorescente y dicho dominio de dirección a la membrana; en el que más del 50% de dicha población de células comprende dichas células que contienen dicho sustrato de toxina clostridial exógeno para la determinación de la actividad de una toxina clostridial.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US66890905P | 2005-04-05 | 2005-04-05 |
Publications (1)
Publication Number | Publication Date |
---|---|
ES2351942T3 true ES2351942T3 (es) | 2011-02-14 |
Family
ID=38829390
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES06851224T Active ES2351942T3 (es) | 2005-04-05 | 2006-04-04 | Analisis de la actividad de una toxina clostridial. |
Country Status (9)
Country | Link |
---|---|
US (1) | US8067231B2 (es) |
EP (2) | EP2264458A1 (es) |
AT (1) | ATE483158T1 (es) |
AU (1) | AU2006340711C1 (es) |
CA (1) | CA2605160A1 (es) |
DE (1) | DE602006017237D1 (es) |
DK (1) | DK1920248T3 (es) |
ES (1) | ES2351942T3 (es) |
WO (1) | WO2008036060A2 (es) |
Families Citing this family (20)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7332567B2 (en) * | 2001-08-28 | 2008-02-19 | Allergan, Inc. | Fret protease assays for clostridial toxins |
CA2504532C (en) * | 2002-10-31 | 2014-09-16 | Wisconsin Alumni Research Foundation | Botulinum neurotoxin b receptors and use thereof |
KR101604515B1 (ko) * | 2008-03-14 | 2016-03-17 | 알러간, 인코포레이티드 | 면역-기반 보툴리눔 독소 세로타입 a 활성 검정 |
PL3031825T3 (pl) | 2008-03-14 | 2020-02-28 | Allergan, Inc. | Immunologiczne oznaczenie aktywności toksyny botulinowej serotypu a |
US8709758B2 (en) * | 2008-07-28 | 2014-04-29 | University Of Louisville Research Foundation, Inc. | Methods and compositions for inhibition of neutrophil exocytosis |
RU2011139364A (ru) | 2009-03-13 | 2013-04-20 | Аллерган, Инк. | Клетки для применения в иммунологических тестах на активность ботулинического токсина серотипа а |
US9303285B2 (en) | 2012-01-04 | 2016-04-05 | Biomadison, Inc. | Methods and compounds for increasing sensitivity of botulinum assays |
CN107475293A (zh) | 2011-06-01 | 2017-12-15 | 拜奥麦迪逊公司 | 非‑fret肉毒梭菌测定法 |
US11325954B2 (en) | 2011-06-01 | 2022-05-10 | Biomadison, Inc. | Compositions and methods for stability testing of botulinum toxin |
US10908146B2 (en) | 2011-06-01 | 2021-02-02 | Biomadison, Inc. | Compositions and methods for improving sensitivity in cell based assays |
KR102007061B1 (ko) * | 2011-12-09 | 2019-08-02 | 앵스띠뛰 파스퇴르 | 다중 면역 스크리닝 분석 |
US10451621B2 (en) | 2011-12-31 | 2019-10-22 | Allergan, Inc. | Highly sensitive cell-based assay to detect the presence of active botulinum neurotoxin serotype-A |
KR20210107179A (ko) | 2012-01-04 | 2021-08-31 | 바이오메디슨, 인코퍼레이티드 | 결합 분석법 |
CA2903809A1 (en) | 2013-03-13 | 2014-10-02 | Tahoe Institute for Rural Health Research, LLC | Portable blood count monitor |
LT3060677T (lt) * | 2013-10-25 | 2018-08-27 | Biomadison, Inc. | Fermentų tyrimo su dublikuotais fluoroforais būdas |
US10408837B2 (en) | 2013-12-09 | 2019-09-10 | The United States Of America, As Represented By The Secretary, Department Of Health & Human Services | Peptide substrates recognizable by type E botulinum neurotoxin |
CN105535975B (zh) * | 2015-12-21 | 2018-05-18 | 中国人民解放军第二军医大学 | 囊泡相关膜蛋白1在防治肠道病毒71型感染中的应用 |
US11129906B1 (en) | 2016-12-07 | 2021-09-28 | David Gordon Bermudes | Chimeric protein toxins for expression by therapeutic bacteria |
WO2019126542A1 (en) | 2017-12-20 | 2019-06-27 | Allergan, Inc. | Botulinum toxin cell binding domain polypeptides and methods of use for treatments of fibrosis associated disorders |
CN116693638B (zh) * | 2023-05-15 | 2024-03-08 | 西北农林科技大学 | Pg1-lc蛋白作为snap-25的水解酶的应用 |
Family Cites Families (51)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5804604A (en) | 1989-12-21 | 1998-09-08 | Biogen, Inc. | Tat-derived transport polypeptides and fusion proteins |
US5670617A (en) | 1989-12-21 | 1997-09-23 | Biogen Inc | Nucleic acid conjugates of tat-derived transport polypeptides |
ES2180529T3 (es) | 1990-02-26 | 2003-02-16 | Univ Leland Stanford Junior | Identificacion y expresion de secuencias de adn de un receptor de esteroides de insectos. |
US5364791A (en) | 1992-05-14 | 1994-11-15 | Elisabetta Vegeto | Progesterone receptor having C. terminal hormone binding domain truncations |
AU685054C (en) | 1992-05-14 | 2003-02-27 | Baylor College Of Medicine | Mutated steroid hormone receptors, methods for their use and molecular switch for gene therapy |
DE69432299T2 (de) | 1993-06-10 | 2003-12-11 | Allergan Inc | Multiple Botulinum Toxine zur Behandlung von neuromuskulären Störungen und Zuständen |
AU689115B2 (en) | 1993-06-10 | 1998-03-26 | Allergan, Inc. | Treatment of neuromuscular disorders and conditions with different botulinum serotype |
US5464758A (en) | 1993-06-14 | 1995-11-07 | Gossen; Manfred | Tight control of gene expression in eucaryotic cells by tetracycline-responsive promoters |
US5814618A (en) | 1993-06-14 | 1998-09-29 | Basf Aktiengesellschaft | Methods for regulating gene expression |
US6974578B1 (en) | 1993-12-28 | 2005-12-13 | Allergan, Inc. | Method for treating secretions and glands using botulinum toxin |
US5962637A (en) | 1994-06-03 | 1999-10-05 | Microbiological Research Authority | Toxin assay |
US5807746A (en) | 1994-06-13 | 1998-09-15 | Vanderbilt University | Method for importing biologically active molecules into cells |
US6495518B1 (en) | 1994-06-13 | 2002-12-17 | Vanderbilt University | Method for importing biologically active molecules into cells |
US5795737A (en) | 1994-09-19 | 1998-08-18 | The General Hospital Corporation | High level expression of proteins |
US5625048A (en) | 1994-11-10 | 1997-04-29 | The Regents Of The University Of California | Modified green fluorescent proteins |
DE69637147T2 (de) | 1995-07-28 | 2008-03-06 | Marie Curie Cancer Care | Transportproteine und deren verwendungen |
FR2739621B1 (fr) | 1995-10-05 | 1997-12-05 | Centre Nat Rech Scient | Peptides utilisables comme vecteurs pour l'adressage intracellulaire de molecules actives |
US5804387A (en) | 1996-02-01 | 1998-09-08 | The Board Of Trustees Of The Leland Stanford Junior University | FACS-optimized mutants of the green fluorescent protein (GFP) |
US6072041A (en) | 1996-06-03 | 2000-06-06 | Case Western Reserve University | Fusion proteins for protein delivery |
US6124128A (en) | 1996-08-16 | 2000-09-26 | The Regents Of The University Of California | Long wavelength engineered fluorescent proteins |
US5965699A (en) | 1996-11-06 | 1999-10-12 | The United States Of America As Represented By The Secretary Of The Army | Assay for the proteolytic activity of serotype a from clostridium botulinum |
US6017735A (en) | 1997-01-23 | 2000-01-25 | Marie Curie Cancer Care | Materials and methods for intracellular transport and their uses |
US6306993B1 (en) | 1997-05-21 | 2001-10-23 | The Board Of Trustees Of The Leland Stanford, Jr. University | Method and composition for enhancing transport across biological membranes |
US6248558B1 (en) | 1998-03-31 | 2001-06-19 | Vanderbilt University | Sequence and method for genetic engineering of proteins with cell membrane translocating activity |
AU3665899A (en) | 1998-04-28 | 1999-11-16 | Washington University | Methods for transducing fusion molecules |
US7090976B2 (en) | 1999-11-10 | 2006-08-15 | Rigel Pharmaceuticals, Inc. | Methods and compositions comprising Renilla GFP |
US6140132A (en) | 1998-06-09 | 2000-10-31 | The Regents Of The University Of California | Fluorescent protein sensors for measuring the pH of a biological sample |
WO2000034308A2 (en) | 1998-12-10 | 2000-06-15 | Washington University | Protein transduction system and methods of use thereof |
EP1157275A4 (en) | 1999-02-28 | 2003-01-15 | Univ Washington | NEW TRANSDUCTION MOLECULES AND METHOD FOR USING THE SAME |
US6767544B2 (en) | 2002-04-01 | 2004-07-27 | Allergan, Inc. | Methods for treating cardiovascular diseases with botulinum toxin |
GB9930519D0 (en) | 1999-12-24 | 2000-02-16 | Phogen Limited | Uses of transport proteins |
US6464986B1 (en) | 2000-04-14 | 2002-10-15 | Allegan Sales, Inc. | Method for treating pain by peripheral administration of a neurotoxin |
FR2809733B1 (fr) | 2000-06-02 | 2004-04-30 | Inst Nat Sante Rech Med | Substrat peptidique reconnu par la toxine botulique de type bont/b et son utilisation pour doser et/ou detecter ladite toxine ou des inhibiteurs correspondants |
US20040219619A1 (en) * | 2000-07-21 | 2004-11-04 | Ester Fernandez-Salas | Methods of identifying compounds that alter toxin persistence and/or protease activity |
US6841535B2 (en) | 2000-07-31 | 2005-01-11 | Active Motif | Peptide-mediated transfection agents and methods of use |
DE60131468D1 (de) | 2000-09-25 | 2007-12-27 | U S Medical Res Inst Of Infect | Hoch-durchsatz assays für proteolytische aktivitäten von clostridium neurotoxinen |
US6423319B1 (en) | 2000-10-04 | 2002-07-23 | Allergan Sales, Inc. | Methods for treating muscle injuries |
US7349691B2 (en) | 2001-07-03 | 2008-03-25 | Microsoft Corporation | System and apparatus for performing broadcast and localcast communications |
US20060063221A1 (en) | 2004-09-21 | 2006-03-23 | Williams Dudley J | Lanthanide-based substrates and methods for determining clostridial toxin activity |
US7374896B2 (en) | 2001-08-28 | 2008-05-20 | Allergan, Inc. | GFP-SNAP25 fluorescence release assay for botulinum neurotoxin protease activity |
US7208285B2 (en) | 2001-08-28 | 2007-04-24 | Allergan, Inc. | Fret protease assays for botulinum serotype A/E toxins |
US7332567B2 (en) | 2001-08-28 | 2008-02-19 | Allergan, Inc. | Fret protease assays for clostridial toxins |
JP4423851B2 (ja) | 2002-02-28 | 2010-03-03 | 日本精工株式会社 | 直動案内装置の製造方法 |
US6921538B2 (en) | 2002-05-10 | 2005-07-26 | Allergan, Inc. | Therapeutic treatments for neuropsychiatric disorders |
AU2002338692A1 (en) * | 2002-09-13 | 2004-05-04 | Otis Elevator Company | Safety monitoring system for a people conveyor |
US7183066B2 (en) | 2002-09-27 | 2007-02-27 | Allergan, Inc. | Cell-based fluorescence resonance energy transfer (FRET) assays for clostridial toxins |
WO2004031355A2 (en) * | 2002-10-01 | 2004-04-15 | University Of Maryland | Methods for identifying inhibitors of botulinum neurotoxins |
US7166692B2 (en) | 2003-03-04 | 2007-01-23 | Canbrex Bio Science Walkersville, Inc. | Intracellular delivery of small molecules, proteins, and nucleic acids |
EP1751284A4 (en) | 2003-12-19 | 2010-01-13 | Wisconsin Alumni Res Found | METHOD AND COMPOSITIONS FOR DETECTING BOTULINUM NEUROTOXINE |
DE602005003817T2 (de) | 2004-05-05 | 2008-12-04 | Pharmaleads | Mittels botulinumtoxin a (bont/a) erkennbare peptidsubstrate und verwendung |
US7399607B2 (en) | 2004-09-22 | 2008-07-15 | Allergan, Inc. | Fluorescence polarization assays for determining clostridial toxin activity |
-
2006
- 2006-04-04 EP EP10167481A patent/EP2264458A1/en not_active Withdrawn
- 2006-04-04 AU AU2006340711A patent/AU2006340711C1/en not_active Ceased
- 2006-04-04 US US11/908,451 patent/US8067231B2/en active Active
- 2006-04-04 EP EP06851224A patent/EP1920248B1/en not_active Revoked
- 2006-04-04 CA CA002605160A patent/CA2605160A1/en not_active Abandoned
- 2006-04-04 ES ES06851224T patent/ES2351942T3/es active Active
- 2006-04-04 AT AT06851224T patent/ATE483158T1/de not_active IP Right Cessation
- 2006-04-04 DE DE602006017237T patent/DE602006017237D1/de active Active
- 2006-04-04 DK DK06851224.3T patent/DK1920248T3/da active
- 2006-04-04 WO PCT/US2006/012825 patent/WO2008036060A2/en active Application Filing
Also Published As
Publication number | Publication date |
---|---|
EP2264458A1 (en) | 2010-12-22 |
AU2006340711A1 (en) | 2007-11-15 |
DK1920248T3 (da) | 2010-12-13 |
US20090191583A1 (en) | 2009-07-30 |
CA2605160A1 (en) | 2006-10-05 |
DE602006017237D1 (en) | 2010-11-11 |
AU2006340711B2 (en) | 2012-02-23 |
EP1920248A2 (en) | 2008-05-14 |
AU2006340711C1 (en) | 2013-02-07 |
ATE483158T1 (de) | 2010-10-15 |
AU2006340711A8 (en) | 2008-08-07 |
WO2008036060A2 (en) | 2008-03-27 |
EP1920248B1 (en) | 2010-09-29 |
WO2008036060A3 (en) | 2008-09-12 |
US8067231B2 (en) | 2011-11-29 |
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