ES2351942T3 - Analisis de la actividad de una toxina clostridial. - Google Patents

Analisis de la actividad de una toxina clostridial. Download PDF

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Publication number
ES2351942T3
ES2351942T3 ES06851224T ES06851224T ES2351942T3 ES 2351942 T3 ES2351942 T3 ES 2351942T3 ES 06851224 T ES06851224 T ES 06851224T ES 06851224 T ES06851224 T ES 06851224T ES 2351942 T3 ES2351942 T3 ES 2351942T3
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Spain
Prior art keywords
clostridial toxin
activity
analysis
cleavage site
fluorescent member
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ES06851224T
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English (en)
Inventor
Ester Fernandez-Salas
Kei Aoki
Lanoe Steward
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Allergan Inc
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Allergan Inc
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Application filed by Allergan Inc filed Critical Allergan Inc
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Publication of ES2351942T3 publication Critical patent/ES2351942T3/es
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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56911—Bacteria
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
    • G01N2333/33—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Clostridium (G)

Abstract

Uso de una composición que comprende una población de células, población que comprende células que contienen un sustrato de toxina clostridial exógeno que puede ser localizado en la membrana plasmática de dichas células; en el que dichas células son capaces de permitir una intoxicación por una toxina clostridial posibilitando el mecanismo celular global mediante el cual una toxina clostridial escinde proteolíticamente un sustrato, y engloba la unión de una toxina clostridial con un receptor de baja o alta afinidad, la interiorización del complejo de toxina/receptor, la translocación de la cadena ligera de la toxina clostridial en el citoplasma y la modificación de la diana enzimática de un sustrato toxina clostridial; en el que las células capaces de permitir una intoxicación por una toxina clostridial son células que expresan uno o más receptores de toxina clostridial de baja o de alta afinidad endógenos para uno o más serotipos de toxina clostridial; expresan uno o más receptores de toxina clostridial de baja o de alta afinidad exógenos para uno o más serotipos de toxina clostridial; o expresan una combinación de receptores de toxina clostridial de baja o alta afinidad endógenos y receptores de toxina clostridial de baja o alta afinidad exógenos para uno o más serotipos de toxina clostridial; en el que dicho sustrato de toxina clostridial exógeno consiste en un miembro fluorescente, en el que dicho miembro fluorescente es un péptido que bien puede absorber inherentemente energía luminosa de una cierta longitud de onda y emitir energía luminosa de una longitud de onda diferente o que forma parte de un complejo que absorbe energía luminosa de una cierta longitud de onda y emite energía luminosa de una longitud de onda diferente; un dominio de dirección a la membrana que dirige el sustrato a la membrana celular; y una secuencia de reconocimiento de toxina clostridial que comprende un sitio de escisión que es un enlace escindible junto con elementos de reconocimiento para la proteolisis detectable en el enlace escindible por parte de una toxina clostridial, en la que el sitio de escisión se encuentra entre dicho miembro fluorescente y dicho dominio de dirección a la membrana; en el que más del 50% de dicha población de células comprende dichas células que contienen dicho sustrato de toxina clostridial exógeno para la determinación de la actividad de una toxina clostridial.
ES06851224T 2005-04-05 2006-04-04 Analisis de la actividad de una toxina clostridial. Active ES2351942T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US66890905P 2005-04-05 2005-04-05

Publications (1)

Publication Number Publication Date
ES2351942T3 true ES2351942T3 (es) 2011-02-14

Family

ID=38829390

Family Applications (1)

Application Number Title Priority Date Filing Date
ES06851224T Active ES2351942T3 (es) 2005-04-05 2006-04-04 Analisis de la actividad de una toxina clostridial.

Country Status (9)

Country Link
US (1) US8067231B2 (es)
EP (2) EP2264458A1 (es)
AT (1) ATE483158T1 (es)
AU (1) AU2006340711C1 (es)
CA (1) CA2605160A1 (es)
DE (1) DE602006017237D1 (es)
DK (1) DK1920248T3 (es)
ES (1) ES2351942T3 (es)
WO (1) WO2008036060A2 (es)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7332567B2 (en) * 2001-08-28 2008-02-19 Allergan, Inc. Fret protease assays for clostridial toxins
CA2504532C (en) * 2002-10-31 2014-09-16 Wisconsin Alumni Research Foundation Botulinum neurotoxin b receptors and use thereof
KR101604515B1 (ko) * 2008-03-14 2016-03-17 알러간, 인코포레이티드 면역-기반 보툴리눔 독소 세로타입 ａ 활성 검정
PL3031825T3 (pl) 2008-03-14 2020-02-28 Allergan, Inc. Immunologiczne oznaczenie aktywności toksyny botulinowej serotypu a
US8709758B2 (en) * 2008-07-28 2014-04-29 University Of Louisville Research Foundation, Inc. Methods and compositions for inhibition of neutrophil exocytosis
RU2011139364A (ru) 2009-03-13 2013-04-20 Аллерган, Инк. Клетки для применения в иммунологических тестах на активность ботулинического токсина серотипа а
US9303285B2 (en) 2012-01-04 2016-04-05 Biomadison, Inc. Methods and compounds for increasing sensitivity of botulinum assays
CN107475293A (zh) 2011-06-01 2017-12-15 拜奥麦迪逊公司 非‑fret肉毒梭菌测定法
US11325954B2 (en) 2011-06-01 2022-05-10 Biomadison, Inc. Compositions and methods for stability testing of botulinum toxin
US10908146B2 (en) 2011-06-01 2021-02-02 Biomadison, Inc. Compositions and methods for improving sensitivity in cell based assays
KR102007061B1 (ko) * 2011-12-09 2019-08-02 앵스띠뛰 파스퇴르 다중 면역 스크리닝 분석
US10451621B2 (en) 2011-12-31 2019-10-22 Allergan, Inc. Highly sensitive cell-based assay to detect the presence of active botulinum neurotoxin serotype-A
KR20210107179A (ko) 2012-01-04 2021-08-31 바이오메디슨, 인코퍼레이티드 결합 분석법
CA2903809A1 (en) 2013-03-13 2014-10-02 Tahoe Institute for Rural Health Research, LLC Portable blood count monitor
LT3060677T (lt) * 2013-10-25 2018-08-27 Biomadison, Inc. Fermentų tyrimo su dublikuotais fluoroforais būdas
US10408837B2 (en) 2013-12-09 2019-09-10 The United States Of America, As Represented By The Secretary, Department Of Health & Human Services Peptide substrates recognizable by type E botulinum neurotoxin
CN105535975B (zh) * 2015-12-21 2018-05-18 中国人民解放军第二军医大学 囊泡相关膜蛋白1在防治肠道病毒71型感染中的应用
US11129906B1 (en) 2016-12-07 2021-09-28 David Gordon Bermudes Chimeric protein toxins for expression by therapeutic bacteria
WO2019126542A1 (en) 2017-12-20 2019-06-27 Allergan, Inc. Botulinum toxin cell binding domain polypeptides and methods of use for treatments of fibrosis associated disorders
CN116693638B (zh) * 2023-05-15 2024-03-08 西北农林科技大学 Pg1-lc蛋白作为snap-25的水解酶的应用

Family Cites Families (51)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5804604A (en) 1989-12-21 1998-09-08 Biogen, Inc. Tat-derived transport polypeptides and fusion proteins
US5670617A (en) 1989-12-21 1997-09-23 Biogen Inc Nucleic acid conjugates of tat-derived transport polypeptides
ES2180529T3 (es) 1990-02-26 2003-02-16 Univ Leland Stanford Junior Identificacion y expresion de secuencias de adn de un receptor de esteroides de insectos.
US5364791A (en) 1992-05-14 1994-11-15 Elisabetta Vegeto Progesterone receptor having C. terminal hormone binding domain truncations
AU685054C (en) 1992-05-14 2003-02-27 Baylor College Of Medicine Mutated steroid hormone receptors, methods for their use and molecular switch for gene therapy
DE69432299T2 (de) 1993-06-10 2003-12-11 Allergan Inc Multiple Botulinum Toxine zur Behandlung von neuromuskulären Störungen und Zuständen
AU689115B2 (en) 1993-06-10 1998-03-26 Allergan, Inc. Treatment of neuromuscular disorders and conditions with different botulinum serotype
US5464758A (en) 1993-06-14 1995-11-07 Gossen; Manfred Tight control of gene expression in eucaryotic cells by tetracycline-responsive promoters
US5814618A (en) 1993-06-14 1998-09-29 Basf Aktiengesellschaft Methods for regulating gene expression
US6974578B1 (en) 1993-12-28 2005-12-13 Allergan, Inc. Method for treating secretions and glands using botulinum toxin
US5962637A (en) 1994-06-03 1999-10-05 Microbiological Research Authority Toxin assay
US5807746A (en) 1994-06-13 1998-09-15 Vanderbilt University Method for importing biologically active molecules into cells
US6495518B1 (en) 1994-06-13 2002-12-17 Vanderbilt University Method for importing biologically active molecules into cells
US5795737A (en) 1994-09-19 1998-08-18 The General Hospital Corporation High level expression of proteins
US5625048A (en) 1994-11-10 1997-04-29 The Regents Of The University Of California Modified green fluorescent proteins
DE69637147T2 (de) 1995-07-28 2008-03-06 Marie Curie Cancer Care Transportproteine und deren verwendungen
FR2739621B1 (fr) 1995-10-05 1997-12-05 Centre Nat Rech Scient Peptides utilisables comme vecteurs pour l'adressage intracellulaire de molecules actives
US5804387A (en) 1996-02-01 1998-09-08 The Board Of Trustees Of The Leland Stanford Junior University FACS-optimized mutants of the green fluorescent protein (GFP)
US6072041A (en) 1996-06-03 2000-06-06 Case Western Reserve University Fusion proteins for protein delivery
US6124128A (en) 1996-08-16 2000-09-26 The Regents Of The University Of California Long wavelength engineered fluorescent proteins
US5965699A (en) 1996-11-06 1999-10-12 The United States Of America As Represented By The Secretary Of The Army Assay for the proteolytic activity of serotype a from clostridium botulinum
US6017735A (en) 1997-01-23 2000-01-25 Marie Curie Cancer Care Materials and methods for intracellular transport and their uses
US6306993B1 (en) 1997-05-21 2001-10-23 The Board Of Trustees Of The Leland Stanford, Jr. University Method and composition for enhancing transport across biological membranes
US6248558B1 (en) 1998-03-31 2001-06-19 Vanderbilt University Sequence and method for genetic engineering of proteins with cell membrane translocating activity
AU3665899A (en) 1998-04-28 1999-11-16 Washington University Methods for transducing fusion molecules
US7090976B2 (en) 1999-11-10 2006-08-15 Rigel Pharmaceuticals, Inc. Methods and compositions comprising Renilla GFP
US6140132A (en) 1998-06-09 2000-10-31 The Regents Of The University Of California Fluorescent protein sensors for measuring the pH of a biological sample
WO2000034308A2 (en) 1998-12-10 2000-06-15 Washington University Protein transduction system and methods of use thereof
EP1157275A4 (en) 1999-02-28 2003-01-15 Univ Washington NEW TRANSDUCTION MOLECULES AND METHOD FOR USING THE SAME
US6767544B2 (en) 2002-04-01 2004-07-27 Allergan, Inc. Methods for treating cardiovascular diseases with botulinum toxin
GB9930519D0 (en) 1999-12-24 2000-02-16 Phogen Limited Uses of transport proteins
US6464986B1 (en) 2000-04-14 2002-10-15 Allegan Sales, Inc. Method for treating pain by peripheral administration of a neurotoxin
FR2809733B1 (fr) 2000-06-02 2004-04-30 Inst Nat Sante Rech Med Substrat peptidique reconnu par la toxine botulique de type bont/b et son utilisation pour doser et/ou detecter ladite toxine ou des inhibiteurs correspondants
US20040219619A1 (en) * 2000-07-21 2004-11-04 Ester Fernandez-Salas Methods of identifying compounds that alter toxin persistence and/or protease activity
US6841535B2 (en) 2000-07-31 2005-01-11 Active Motif Peptide-mediated transfection agents and methods of use
DE60131468D1 (de) 2000-09-25 2007-12-27 U S Medical Res Inst Of Infect Hoch-durchsatz assays für proteolytische aktivitäten von clostridium neurotoxinen
US6423319B1 (en) 2000-10-04 2002-07-23 Allergan Sales, Inc. Methods for treating muscle injuries
US7349691B2 (en) 2001-07-03 2008-03-25 Microsoft Corporation System and apparatus for performing broadcast and localcast communications
US20060063221A1 (en) 2004-09-21 2006-03-23 Williams Dudley J Lanthanide-based substrates and methods for determining clostridial toxin activity
US7374896B2 (en) 2001-08-28 2008-05-20 Allergan, Inc. GFP-SNAP25 fluorescence release assay for botulinum neurotoxin protease activity
US7208285B2 (en) 2001-08-28 2007-04-24 Allergan, Inc. Fret protease assays for botulinum serotype A/E toxins
US7332567B2 (en) 2001-08-28 2008-02-19 Allergan, Inc. Fret protease assays for clostridial toxins
JP4423851B2 (ja) 2002-02-28 2010-03-03 日本精工株式会社 直動案内装置の製造方法
US6921538B2 (en) 2002-05-10 2005-07-26 Allergan, Inc. Therapeutic treatments for neuropsychiatric disorders
AU2002338692A1 (en) * 2002-09-13 2004-05-04 Otis Elevator Company Safety monitoring system for a people conveyor
US7183066B2 (en) 2002-09-27 2007-02-27 Allergan, Inc. Cell-based fluorescence resonance energy transfer (FRET) assays for clostridial toxins
WO2004031355A2 (en) * 2002-10-01 2004-04-15 University Of Maryland Methods for identifying inhibitors of botulinum neurotoxins
US7166692B2 (en) 2003-03-04 2007-01-23 Canbrex Bio Science Walkersville, Inc. Intracellular delivery of small molecules, proteins, and nucleic acids
EP1751284A4 (en) 2003-12-19 2010-01-13 Wisconsin Alumni Res Found METHOD AND COMPOSITIONS FOR DETECTING BOTULINUM NEUROTOXINE
DE602005003817T2 (de) 2004-05-05 2008-12-04 Pharmaleads Mittels botulinumtoxin a (bont/a) erkennbare peptidsubstrate und verwendung
US7399607B2 (en) 2004-09-22 2008-07-15 Allergan, Inc. Fluorescence polarization assays for determining clostridial toxin activity

Also Published As

Publication number Publication date
EP2264458A1 (en) 2010-12-22
AU2006340711A1 (en) 2007-11-15
DK1920248T3 (da) 2010-12-13
US20090191583A1 (en) 2009-07-30
CA2605160A1 (en) 2006-10-05
DE602006017237D1 (en) 2010-11-11
AU2006340711B2 (en) 2012-02-23
EP1920248A2 (en) 2008-05-14
AU2006340711C1 (en) 2013-02-07
ATE483158T1 (de) 2010-10-15
AU2006340711A8 (en) 2008-08-07
WO2008036060A2 (en) 2008-03-27
EP1920248B1 (en) 2010-09-29
WO2008036060A3 (en) 2008-09-12
US8067231B2 (en) 2011-11-29

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