MX2011011045A - Medios y metodos para determinacion de cantidad de polipeptido de neurotoxina y de sus actividades cataliticas y proteoliticas. - Google Patents

Medios y metodos para determinacion de cantidad de polipeptido de neurotoxina y de sus actividades cataliticas y proteoliticas.

Info

Publication number
MX2011011045A
MX2011011045A MX2011011045A MX2011011045A MX2011011045A MX 2011011045 A MX2011011045 A MX 2011011045A MX 2011011045 A MX2011011045 A MX 2011011045A MX 2011011045 A MX2011011045 A MX 2011011045A MX 2011011045 A MX2011011045 A MX 2011011045A
Authority
MX
Mexico
Prior art keywords
amount
neurotoxin polypeptide
catalytic
determination
methods
Prior art date
Application number
MX2011011045A
Other languages
English (en)
Inventor
Michael Pfeil
Josef Friedrich
Original Assignee
Merz Pharma Gmbh & Co Kgaa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Merz Pharma Gmbh & Co Kgaa filed Critical Merz Pharma Gmbh & Co Kgaa
Publication of MX2011011045A publication Critical patent/MX2011011045A/es

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54306—Solid-phase reaction mechanisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56911—Bacteria
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6854—Immunoglobulins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
    • G01N2333/33—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Clostridium (G)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Abstract

La presente invención pertenece al campo de las herramientas para asegurar la elaboración de polipéptidos y control de calidad. Específicamente, se relaciona con un método para determinar la cantidad de polipéptido de neurotoxina procesada (activa) en una solución que comprende el polipéptido de neurotoxina procesada y el polipéptido de neurotoxina parcialmente procesada o sin procesar. La presente invención se relaciona además con un dispositivo para determinar la cantidad y un kit adaptado para realizar el método de la presente invención.
MX2011011045A 2009-04-27 2010-04-23 Medios y metodos para determinacion de cantidad de polipeptido de neurotoxina y de sus actividades cataliticas y proteoliticas. MX2011011045A (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US21465009P 2009-04-27 2009-04-27
EP09158788 2009-04-27
PCT/EP2010/055432 WO2010124998A1 (en) 2009-04-27 2010-04-23 Means and methods for the determination of the amount of neurotoxin polypeptide and of its catalytic and proteolytic activities

Publications (1)

Publication Number Publication Date
MX2011011045A true MX2011011045A (es) 2011-11-04

Family

ID=41066304

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2011011045A MX2011011045A (es) 2009-04-27 2010-04-23 Medios y metodos para determinacion de cantidad de polipeptido de neurotoxina y de sus actividades cataliticas y proteoliticas.

Country Status (15)

Country Link
US (2) US8663942B2 (es)
EP (1) EP2425251B1 (es)
JP (1) JP5689113B2 (es)
KR (1) KR101818777B1 (es)
CN (1) CN102414564B (es)
AU (1) AU2010243692B2 (es)
BR (1) BRPI1015311B1 (es)
CA (1) CA2757637C (es)
ES (1) ES2647243T3 (es)
HK (1) HK1169171A1 (es)
IL (1) IL215441A (es)
MX (1) MX2011011045A (es)
PT (1) PT2425251T (es)
RU (1) RU2545783C9 (es)
WO (1) WO2010124998A1 (es)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2544060T3 (es) * 2010-08-11 2015-08-27 Merz Pharma Gmbh & Co. Kgaa Fabricación selectiva de polipéptidos de neurotoxina recombinantes
JP6156954B2 (ja) 2012-11-21 2017-07-05 イプセン バイオイノベーション リミテッド タンパク分解性にプロセシングされたポリペプチドの製造方法
JP6608357B2 (ja) * 2013-06-28 2019-11-20 メルツ ファルマ ゲーエムベーハー ウント コンパニー カーゲーアーアー 細胞において神経毒ポリペプチドの生物活性を決定する手段及び方法
US10634683B2 (en) 2014-11-21 2020-04-28 Merz Pharma Gmbh & Co. Kgaa Methods for the determination of the biological activities of neurotoxin polypeptides
RU2694964C2 (ru) * 2017-12-19 2019-07-19 ФЕДЕРАЛЬНОЕ АГЕНТСТВО ПО ТЕХНИЧЕСКОМУ РЕГУЛИРОВАНИЮ И МЕТРОЛОГИИ (Росстандарт) Способ измерений каталитической активности (каталитической концентрации) ферментов с использованием методов масс-спектрометрии и спектрофотометрии с предварительной идентификацией целевого аналита и определением чистоты веществ
JP7394767B2 (ja) 2018-01-12 2023-12-08 ジェンザイム・コーポレーション ポリペプチドの定量化方法
WO2020206375A1 (en) * 2019-04-03 2020-10-08 Technical University Of Denmark Neurotrophic factor protein conjugates and related embodiments

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US4208479A (en) * 1977-07-14 1980-06-17 Syva Company Label modified immunoassays
DE69033457T2 (de) * 1989-08-16 2000-09-14 Chiron Corp Prohormonspaltungsplatzblockierungsantikörper
GB9411138D0 (en) * 1994-06-03 1994-07-27 Microbiological Res Authority Toxin assay
DE60032367T3 (de) * 1999-08-25 2011-03-10 Allergan, Inc., Irvine Aktivierbare rekombinante neurotoxine
US6678651B2 (en) 2000-09-15 2004-01-13 Mindspeed Technologies, Inc. Short-term enhancement in CELP speech coding
US20060063221A1 (en) * 2004-09-21 2006-03-23 Williams Dudley J Lanthanide-based substrates and methods for determining clostridial toxin activity
US7332567B2 (en) * 2001-08-28 2008-02-19 Allergan, Inc. Fret protease assays for clostridial toxins
JP5178009B2 (ja) * 2003-12-19 2013-04-10 ウィスコンシン アルムニ リサーチ ファンデイション ボツリヌス神経毒素検出のための方法および複合体
FR2869908B1 (fr) * 2004-05-05 2006-07-28 Pharmaleads Substrat peptidique reconnu par la toxine botulique de type a, bont/a et son utilisation pour detecter et/ou doser ladite toxine ou des inhibiteurs de son activite
KR20100020972A (ko) * 2007-06-01 2010-02-23 메르츠 파마 게엠베하 운트 코. 카가아 보툴리늄 톡신의 신경독 성분에 기초한 온도-안정한 근육 이완제를 제공하는 방법
WO2008157374A1 (en) * 2007-06-13 2008-12-24 The United States Of America, As Represented By The Secretrary Of Agriculture High-affinity monoclonal antibodies for botulinum toxin type a
EP2178905B1 (en) * 2007-07-26 2013-11-06 Allergan, Inc. Methods of activiting clostridial toxins
CN101386648B (zh) * 2008-09-25 2012-05-30 中国人民解放军军事医学科学院微生物流行病研究所 抗b型肉毒神经毒素中和性单克隆抗体、其制备方法及用途
MX340772B (es) * 2009-02-19 2016-07-26 Merz Pharma Gmbh & Co Kgaa Medios y metodos para fabricar neurotoxina altamente pura.

Also Published As

Publication number Publication date
CN102414564A (zh) 2012-04-11
RU2545783C9 (ru) 2016-02-27
CA2757637C (en) 2017-10-24
AU2010243692A1 (en) 2011-10-27
IL215441A0 (en) 2011-12-29
ES2647243T3 (es) 2017-12-20
AU2010243692B2 (en) 2015-03-05
HK1169171A1 (en) 2013-01-18
RU2011147994A (ru) 2013-06-10
IL215441A (en) 2015-04-30
US20120142024A1 (en) 2012-06-07
EP2425251B1 (en) 2017-10-25
US8663942B2 (en) 2014-03-04
PT2425251T (pt) 2017-11-27
KR20120005041A (ko) 2012-01-13
CN102414564B (zh) 2015-01-21
JP2012525563A (ja) 2012-10-22
CA2757637A1 (en) 2010-11-04
KR101818777B1 (ko) 2018-01-15
US20140134643A1 (en) 2014-05-15
BRPI1015311B1 (pt) 2024-01-09
EP2425251A1 (en) 2012-03-07
BRPI1015311A2 (pt) 2016-04-19
JP5689113B2 (ja) 2015-03-25
RU2545783C2 (ru) 2015-04-10
WO2010124998A1 (en) 2010-11-04

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