DK1807698T3 - Assay til bestemmelse af aktiviteten af clostridialt toksin ved kombination af FRET og fluorescenspolarisering - Google Patents
Assay til bestemmelse af aktiviteten af clostridialt toksin ved kombination af FRET og fluorescenspolariseringInfo
- Publication number
- DK1807698T3 DK1807698T3 DK05858107.5T DK05858107T DK1807698T3 DK 1807698 T3 DK1807698 T3 DK 1807698T3 DK 05858107 T DK05858107 T DK 05858107T DK 1807698 T3 DK1807698 T3 DK 1807698T3
- Authority
- DK
- Denmark
- Prior art keywords
- clostridial toxin
- activity
- fluorescence polarization
- fluorophore
- fret
- Prior art date
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
- G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
- C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
- G01N2333/33—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Clostridium (G)
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Organic Chemistry (AREA)
- Molecular Biology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- Hematology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Urology & Nephrology (AREA)
- Medicinal Chemistry (AREA)
- General Engineering & Computer Science (AREA)
- Cell Biology (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Biophysics (AREA)
- Food Science & Technology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Peptides Or Proteins (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Prostheses (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US10/948,097 US7399607B2 (en) | 2004-09-22 | 2004-09-22 | Fluorescence polarization assays for determining clostridial toxin activity |
PCT/US2005/032328 WO2007001358A2 (en) | 2004-09-22 | 2005-09-09 | Fluorescence polarization assays for determining clostridial toxin activity |
Publications (1)
Publication Number | Publication Date |
---|---|
DK1807698T3 true DK1807698T3 (da) | 2010-09-20 |
Family
ID=36074524
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DK05858107.5T DK1807698T3 (da) | 2004-09-22 | 2005-09-09 | Assay til bestemmelse af aktiviteten af clostridialt toksin ved kombination af FRET og fluorescenspolarisering |
Country Status (11)
Country | Link |
---|---|
US (7) | US7399607B2 (da) |
EP (2) | EP2107112A1 (da) |
JP (2) | JP2008513038A (da) |
KR (1) | KR101442246B1 (da) |
AT (1) | ATE472101T1 (da) |
AU (1) | AU2005333632B2 (da) |
CA (1) | CA2581231A1 (da) |
DE (1) | DE602005021981D1 (da) |
DK (1) | DK1807698T3 (da) |
ES (2) | ES2345780T3 (da) |
WO (1) | WO2007001358A2 (da) |
Families Citing this family (29)
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US7332567B2 (en) * | 2001-08-28 | 2008-02-19 | Allergan, Inc. | Fret protease assays for clostridial toxins |
US8022172B2 (en) * | 2001-08-28 | 2011-09-20 | Allergan, Inc. | Luminescence resonance energy transfer (LRET) assays for clostridial toxin activity |
US7208285B2 (en) * | 2001-08-28 | 2007-04-24 | Allergan, Inc. | Fret protease assays for botulinum serotype A/E toxins |
US7374896B2 (en) * | 2001-08-28 | 2008-05-20 | Allergan, Inc. | GFP-SNAP25 fluorescence release assay for botulinum neurotoxin protease activity |
US20080064054A1 (en) * | 2001-08-28 | 2008-03-13 | Ester Fernandez-Salas | Fluorescence resonance energy transfer (fret) assays for clostridial toxin activity |
US7183066B2 (en) * | 2002-09-27 | 2007-02-27 | Allergan, Inc. | Cell-based fluorescence resonance energy transfer (FRET) assays for clostridial toxins |
US7399607B2 (en) | 2004-09-22 | 2008-07-15 | Allergan, Inc. | Fluorescence polarization assays for determining clostridial toxin activity |
WO2008036060A2 (en) * | 2005-04-05 | 2008-03-27 | Allergan, Inc. | Clostridial toxin activity assays |
PT1869459E (pt) * | 2005-04-05 | 2010-08-10 | Allergan Inc | Ensaios fret à base de corantes lipofílicos para a actividade da toxina de clostrídeo |
DE602006013481D1 (de) * | 2005-09-19 | 2010-05-20 | Allergan Inc | Mit clostriedientoxin aktivierbare clostridientoxine |
CA2602972C (en) * | 2005-10-12 | 2013-09-24 | Allergan, Inc. | Assays of molecular or subcellular interactivity using depolarization after resonance energy transfer (daret) |
US8003314B2 (en) | 2007-04-16 | 2011-08-23 | Diagnostic Hybrids, Inc. | Methods for direct fluorescent antibody virus detection in liquids |
US8753831B2 (en) * | 2007-06-05 | 2014-06-17 | City Of Hope | Methods for detection of botulinum neurotoxin |
EP2170400B1 (en) * | 2007-07-10 | 2012-07-25 | Medy-Tox, INC. | Pharmaceutical liquid composition of botulinum toxin with improved stability |
KR101609894B1 (ko) | 2008-03-14 | 2016-04-08 | 알러간, 인코포레이티드 | 면역-기반 보툴리눔 독소 세로타입 a 활성 검정 |
KR101604515B1 (ko) * | 2008-03-14 | 2016-03-17 | 알러간, 인코포레이티드 | 면역-기반 보툴리눔 독소 세로타입 a 활성 검정 |
RU2543650C2 (ru) * | 2009-03-13 | 2015-03-10 | Аллерган, Инк. | Иммунологические тесты на активность эндопептидаз с измененной нацеленностью |
KR20200146046A (ko) | 2009-03-13 | 2020-12-31 | 알러간, 인코포레이티드 | 면역 기반 보툴리눔 독소 혈청형 a 활성 검정에 유용한 세포 |
US8129139B2 (en) | 2009-07-13 | 2012-03-06 | Allergan, Inc. | Process for obtaining botulinum neurotoxin |
EP2483666A1 (en) | 2009-10-02 | 2012-08-08 | Allergan, Inc. | In vitro method of determining changes in a protein environment |
WO2013102088A2 (en) | 2011-12-31 | 2013-07-04 | Allergan, Inc. | Highly Sensitive Cell-Based Assay to Detect the Presence of Active Botulinum Neurotoxin Serotype-A |
KR102607322B1 (ko) | 2012-01-04 | 2023-11-29 | 바이오메디슨, 인코퍼레이티드 | 보툴리눔 분석 감도를 증진시키기 위한 방법 및 화합물 |
US8962340B2 (en) | 2012-12-03 | 2015-02-24 | Src, Inc. | Real-time assay for the detection of botulinum toxin |
EP3270162B2 (en) | 2013-08-09 | 2023-08-16 | BioMadison, Inc. | Botulinum toxin assay with improved sensitivity |
KR20170026624A (ko) | 2014-07-07 | 2017-03-08 | 알러간, 인코포레이티드 | 조직 샘플에서 절단된 snap25를 검출하는 방법 |
FR3053689B1 (fr) * | 2016-07-06 | 2021-01-08 | Univ Claude Bernard Lyon | Polypeptides substrats des proteases de la famille des astacines et leurs utilisations |
AU2017327369A1 (en) | 2016-09-13 | 2019-05-02 | Allergan, Inc. | Stabilized non-protein Clostridial toxin compositions |
WO2018073370A1 (en) * | 2016-10-21 | 2018-04-26 | Toxogen Gmbh | A functional detection assay devoid of antibodies for serotyping of botulinum neurotoxins |
US11129906B1 (en) | 2016-12-07 | 2021-09-28 | David Gordon Bermudes | Chimeric protein toxins for expression by therapeutic bacteria |
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CA2602972C (en) * | 2005-10-12 | 2013-09-24 | Allergan, Inc. | Assays of molecular or subcellular interactivity using depolarization after resonance energy transfer (daret) |
-
2004
- 2004-09-22 US US10/948,097 patent/US7399607B2/en active Active
-
2005
- 2005-09-09 AT AT05858107T patent/ATE472101T1/de not_active IP Right Cessation
- 2005-09-09 ES ES05858107T patent/ES2345780T3/es active Active
- 2005-09-09 DE DE602005021981T patent/DE602005021981D1/de active Active
- 2005-09-09 EP EP09160607A patent/EP2107112A1/en not_active Withdrawn
- 2005-09-09 KR KR1020077009002A patent/KR101442246B1/ko active IP Right Grant
- 2005-09-09 CA CA002581231A patent/CA2581231A1/en not_active Abandoned
- 2005-09-09 WO PCT/US2005/032328 patent/WO2007001358A2/en active Application Filing
- 2005-09-09 DK DK05858107.5T patent/DK1807698T3/da active
- 2005-09-09 JP JP2007533521A patent/JP2008513038A/ja active Pending
- 2005-09-09 AU AU2005333632A patent/AU2005333632B2/en not_active Ceased
- 2005-09-09 EP EP05858107A patent/EP1807698B1/en not_active Revoked
-
2008
- 2008-05-21 US US12/124,412 patent/US7638294B2/en active Active
- 2008-05-22 US US12/125,235 patent/US7635574B2/en active Active
- 2008-05-22 US US12/125,327 patent/US7674601B2/en active Active
- 2008-05-22 US US12/125,723 patent/US7632655B2/en active Active
-
2009
- 2009-04-23 ES ES21203994T patent/ES2928863T3/es active Active
- 2009-10-22 US US12/604,059 patent/US20100081155A1/en not_active Abandoned
- 2009-10-22 US US12/604,138 patent/US20100081156A1/en not_active Abandoned
-
2012
- 2012-05-31 JP JP2012125033A patent/JP2012210215A/ja active Pending
Also Published As
Publication number | Publication date |
---|---|
US20100081156A1 (en) | 2010-04-01 |
US7399607B2 (en) | 2008-07-15 |
US7632655B2 (en) | 2009-12-15 |
US20080305509A1 (en) | 2008-12-11 |
ES2928863T3 (es) | 2022-11-23 |
US20080293085A1 (en) | 2008-11-27 |
CA2581231A1 (en) | 2007-01-04 |
EP2107112A1 (en) | 2009-10-07 |
DE602005021981D1 (de) | 2010-08-05 |
US7635574B2 (en) | 2009-12-22 |
ATE472101T1 (de) | 2010-07-15 |
US20080293084A1 (en) | 2008-11-27 |
ES2345780T3 (es) | 2010-10-01 |
AU2005333632A1 (en) | 2007-01-04 |
US20100081155A1 (en) | 2010-04-01 |
US7638294B2 (en) | 2009-12-29 |
WO2007001358A3 (en) | 2007-06-28 |
KR20070055609A (ko) | 2007-05-30 |
JP2012210215A (ja) | 2012-11-01 |
WO2007001358A2 (en) | 2007-01-04 |
EP1807698A2 (en) | 2007-07-18 |
JP2008513038A (ja) | 2008-05-01 |
KR101442246B1 (ko) | 2014-10-01 |
AU2005333632B2 (en) | 2011-12-22 |
US7674601B2 (en) | 2010-03-09 |
EP1807698B1 (en) | 2010-06-23 |
US20060063222A1 (en) | 2006-03-23 |
US20080305510A1 (en) | 2008-12-11 |
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