BR0314795A - Testes de transferência de energia de ressonância de fluorescência (fret) à base de células para toxinas clostridianas - Google Patents

Testes de transferência de energia de ressonância de fluorescência (fret) à base de células para toxinas clostridianas

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Publication number
BR0314795A
BR0314795A BR0314795-9A BR0314795A BR0314795A BR 0314795 A BR0314795 A BR 0314795A BR 0314795 A BR0314795 A BR 0314795A BR 0314795 A BR0314795 A BR 0314795A
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BR
Brazil
Prior art keywords
energy transfer
resonance energy
cell
donor fluorophore
clostridial toxin
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Application number
BR0314795-9A
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English (en)
Inventor
Ester Fernandez-Salas
Lance E Steward
Kei Roger Aoki
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Allergan Inc
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Application filed by Allergan Inc filed Critical Allergan Inc
Publication of BR0314795A publication Critical patent/BR0314795A/pt

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    • G—PHYSICS
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    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
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    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
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    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
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Abstract

"TESTES DE TRANSFERêNCIA DE ENERGIA DE RESSONâNCIA DE FLUORESCêNCIA (FRET) à BASE DE CéLULAS PARA TOXINAS CLOSTRIDIANAS". A presente invenção refere-se a um método para determinar a atividade de toxina clostridiana por (a) contatar com uma amostra uma célula contendo um substrato de toxina clostridiana que inclui um fluoróforo doador; um aceitador tendo um espectro de absorbância soprepondo o espectro de emissão do referido fluoróforo doador; e uma seq³ência de reconhecimento de toxina clostridiana contendo um sítio de clivagem que intervém entre fluoróforo doador e um aceitador, onde a transferência de energia de ressonância é demonstrada entre fluoróforo doador e um aceitador sob as condições apropriadas; (b) excitar o fluoróforo doador; e (c) determinar a transferência de energia de ressonância da célula contatada com relação a uma célula de controle, onde a diferença na transferência de energia de ressonância da célula contatada como comparada com a célula de controle é indicativa de atividade de toxina clostridiana.
BR0314795-9A 2002-09-27 2003-09-04 Testes de transferência de energia de ressonância de fluorescência (fret) à base de células para toxinas clostridianas BR0314795A (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US10/261,161 US7183066B2 (en) 2002-09-27 2002-09-27 Cell-based fluorescence resonance energy transfer (FRET) assays for clostridial toxins
PCT/US2003/028092 WO2004029576A2 (en) 2002-09-27 2003-09-04 Cell-based fluorescence resonance energy transfer (fret) assays for clostridial toxins

Publications (1)

Publication Number Publication Date
BR0314795A true BR0314795A (pt) 2005-07-26

Family

ID=32041812

Family Applications (1)

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BR0314795-9A BR0314795A (pt) 2002-09-27 2003-09-04 Testes de transferência de energia de ressonância de fluorescência (fret) à base de células para toxinas clostridianas

Country Status (17)

Country Link
US (3) US7183066B2 (pt)
EP (1) EP1543329B1 (pt)
JP (1) JP4554369B2 (pt)
KR (1) KR20050071506A (pt)
CN (1) CN1296712C (pt)
AU (2) AU2003282790B2 (pt)
BR (1) BR0314795A (pt)
CA (1) CA2500040C (pt)
CO (1) CO5721032A2 (pt)
DK (1) DK1543329T3 (pt)
IL (1) IL167410A (pt)
MX (1) MXPA05003128A (pt)
NO (1) NO20051049L (pt)
PL (1) PL376103A1 (pt)
RU (1) RU2005109385A (pt)
WO (1) WO2004029576A2 (pt)
ZA (1) ZA200501500B (pt)

Families Citing this family (68)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080038274A1 (en) * 1999-09-23 2008-02-14 Foster Keith A Inhibition of secretion from non-neuronal cells
US7354735B2 (en) * 2001-05-24 2008-04-08 Hong Kong University Of Science And Technology Nucleic acid encoding fluorescent protein constructs and methods for detecting apoptosis
US7374896B2 (en) * 2001-08-28 2008-05-20 Allergan, Inc. GFP-SNAP25 fluorescence release assay for botulinum neurotoxin protease activity
US7208285B2 (en) * 2001-08-28 2007-04-24 Allergan, Inc. Fret protease assays for botulinum serotype A/E toxins
US20080064054A1 (en) * 2001-08-28 2008-03-13 Ester Fernandez-Salas Fluorescence resonance energy transfer (fret) assays for clostridial toxin activity
US20060063221A1 (en) * 2004-09-21 2006-03-23 Williams Dudley J Lanthanide-based substrates and methods for determining clostridial toxin activity
US7332567B2 (en) * 2001-08-28 2008-02-19 Allergan, Inc. Fret protease assays for clostridial toxins
US8022172B2 (en) * 2001-08-28 2011-09-20 Allergan, Inc. Luminescence resonance energy transfer (LRET) assays for clostridial toxin activity
US7183066B2 (en) * 2002-09-27 2007-02-27 Allergan, Inc. Cell-based fluorescence resonance energy transfer (FRET) assays for clostridial toxins
AU2003272800A1 (en) * 2002-10-01 2004-04-23 University Of Maryland Methods for identifying inhibitors of botulinum neurotoxins
US8137924B2 (en) * 2003-12-19 2012-03-20 Wisconsin Alumni Research Foundation Method and compositions for detecting botulinum neurotoxin
WO2005082096A2 (en) * 2004-02-24 2005-09-09 Allergan, Inc. Botulinum toxin screening assays
US8497081B2 (en) 2004-02-24 2013-07-30 Allergan, Inc. Botulinum toxin screening assays
AU2005279741B2 (en) * 2004-09-01 2011-10-06 Allergan, Inc. Degradable clostridial toxins
WO2006101520A2 (en) * 2004-09-20 2006-09-28 University Of Massachusetts Fluorescent proteins and related methods and compounds
US7399607B2 (en) * 2004-09-22 2008-07-15 Allergan, Inc. Fluorescence polarization assays for determining clostridial toxin activity
US8031338B2 (en) * 2004-12-02 2011-10-04 Vanderbilt University Measuring Forster resonance energy transfer with polarized and depolarized light
ES2351942T3 (es) 2005-04-05 2011-02-14 Allergan Inc Analisis de la actividad de una toxina clostridial.
ES2345506T3 (es) * 2005-04-05 2010-09-24 Allergan, Inc. Ensayos de fret a base de colorantes lipofilos para determinar la actividad de la toxina clostridial.
DE102005020384A1 (de) * 2005-05-02 2006-11-09 Therainvention Gmbh Spektroskopisches Verfahren zum Nachweis von Analyten
CA2610103A1 (en) * 2005-09-19 2007-03-19 Allergan, Inc. Clostridial toxin activatable clostridial toxins
ATE456798T1 (de) * 2005-10-12 2010-02-15 Allergan Inc Tests der molekularen oder subzellulären interaktivität unter verwendung von depolarisierung nach resonanzenergietransfer (daret)
FR2903495A1 (fr) * 2006-07-06 2008-01-11 Pharmaleads Soc Par Actions Si PROCEDE DE DETECTION D'INTERACTIONS A L'AIDE DU RAPPORTEUR DE FLUORESCENCE Pya
JP2008220302A (ja) * 2007-03-14 2008-09-25 Hiroshima Univ タンパク質蓄積による神経変性疾患治療薬物のスクリーニング系
US7733470B2 (en) * 2007-06-26 2010-06-08 Photon Technology International, Inc. Method and apparatus for real-time measurement and calculation of a fluorescent lifetime
CA2699612C (en) 2007-09-14 2013-01-08 Biosentinel, Llc Resonance energy transfer assay with cleavage sequence and spacer
ES2524312T3 (es) 2008-03-14 2014-12-05 Allergan, Inc. Ensayos de actividad de serotipo A de toxina botulínica de base inmunológica
KR101604515B1 (ko) * 2008-03-14 2016-03-17 알러간, 인코포레이티드 면역-기반 보툴리눔 독소 세로타입 ａ 활성 검정
KR20110106346A (ko) 2008-12-10 2011-09-28 알러간, 인코포레이티드 클로스트리디움 독소 약제학적 조성물
US8492109B2 (en) * 2009-01-20 2013-07-23 Trustees Of Tufts College Methods for the delivery of toxins or enzymatically active portions thereof
SG174353A1 (en) 2009-03-13 2011-11-28 Allergan Inc Immuno-based retargeted endopeptidase activity assays
KR102017327B1 (ko) 2009-03-13 2019-09-03 알러간, 인코포레이티드 면역 기반 보툴리눔 독소 혈청형 ａ 활성 검정에 유용한 세포
US9247611B2 (en) * 2009-06-01 2016-01-26 Apple Inc. Light source with light sensor
US8129139B2 (en) 2009-07-13 2012-03-06 Allergan, Inc. Process for obtaining botulinum neurotoxin
WO2011039953A1 (ja) * 2009-09-29 2011-04-07 三井造船株式会社 Ｆｒｅｔ測定方法及び装置
EP2483666A1 (en) 2009-10-02 2012-08-08 Allergan, Inc. In vitro method of determining changes in a protein environment
FR2958293B1 (fr) 2010-04-01 2014-09-26 Centre Nat Rech Scient Outils pour l'identification de ligands de lingo-1,lingo-2, lingo-3 et lingo-4, et utilisations
CN102243176B (zh) * 2010-05-14 2013-11-20 中国人民解放军军事医学科学院毒物药物研究所 一种体外分子水平荧光法排除筛选药物假阳性结果的方法
EP2580345A4 (en) * 2010-06-11 2014-06-18 Synaptic Res Llc METHODS OF INDICATING N-TERMINAL RULE PROTEASE ACTIVITY
CN103228669B (zh) * 2010-09-27 2016-02-24 国立大学法人京都大学 基于荧光共振能量转移的原理的单分子型fret生物传感器接头
US8569254B2 (en) 2010-12-10 2013-10-29 National Yang Ming University Methods for modulating the expression and aggregation of CAG-expanded gene product in cells and methods for identifying agents useful for doing the same
US10908146B2 (en) 2011-06-01 2021-02-02 Biomadison, Inc. Compositions and methods for improving sensitivity in cell based assays
EP2715355B1 (en) * 2011-06-01 2017-03-08 Biomadison, Inc. Non-fret botulinum assay
US9303285B2 (en) 2012-01-04 2016-04-05 Biomadison, Inc. Methods and compounds for increasing sensitivity of botulinum assays
US11325954B2 (en) 2011-06-01 2022-05-10 Biomadison, Inc. Compositions and methods for stability testing of botulinum toxin
WO2013102088A2 (en) 2011-12-31 2013-07-04 Allergan, Inc. Highly Sensitive Cell-Based Assay to Detect the Presence of Active Botulinum Neurotoxin Serotype-A
WO2013103983A1 (en) * 2012-01-06 2013-07-11 The Research Foundation Of State University Of New York High throughput screening of live cells
GB201219024D0 (en) * 2012-10-23 2012-12-05 Syntaxin Ltd Assay
WO2015021433A1 (en) 2013-08-09 2015-02-12 Biomadison, Inc. Botulinum toxin assay with improved sensitivity
LT3060677T (lt) 2013-10-25 2018-08-27 Biomadison, Inc. Fermentų tyrimo su dublikuotais fluoroforais būdas
JP6630717B2 (ja) 2014-07-07 2020-01-15 アラーガン、インコーポレイテッドＡｌｌｅｒｇａｎ，Ｉｎｃｏｒｐｏｒａｔｅｄ 組織試料における切断ｓｎａｐ２５の検出方法
AU2015301737B2 (en) 2014-08-12 2021-01-28 Biomadison, Inc. Botulinum neurotoxins with modified light chain specifity and methods for producing same
CN104359883A (zh) * 2014-11-13 2015-02-18 贵阳医学院 人源沉默信息调节因子6的活性荧光检测方法
SG11201705019PA (en) 2014-12-23 2017-07-28 Merz Pharma Gmbh & Co Kgaa Botulinum toxin prefilled container
CN105784656B (zh) * 2016-03-16 2019-01-01 大连理工大学 一种检测活细胞内RhoGDIα蛋白活性的生物探针
TWI737742B (zh) 2016-06-22 2021-09-01 德商梅茲製藥有限兩合公司 肉毒桿菌毒素的預填充式注射器系統、具有其之套組以及其之使用
AU2017326253B2 (en) 2016-09-13 2021-10-21 Allergan, Inc. Stabilized non-protein clostridial toxin compositions
US11129906B1 (en) 2016-12-07 2021-09-28 David Gordon Bermudes Chimeric protein toxins for expression by therapeutic bacteria
CN108305912B (zh) * 2017-01-11 2024-03-26 中国科学院上海微系统与信息技术研究所 具有波长选择性的石墨烯仿生光探测器及其制备方法
BR112019025394A2 (pt) * 2017-06-07 2020-06-30 Original G B.V. método para detectar um micróbio de deterioração de alimentos e kit para a detecção de microrganismos de deterioração de alimentos
JP7316266B2 (ja) * 2018-03-09 2023-07-27 ザルトリウス バイオアナリティカル インストゥルメンツ， インコーポレイテッド 細胞内ａｔｐの生細胞分析のための方法と組成物
CN108333342A (zh) * 2018-05-14 2018-07-27 广东药科大学 一种快速检测牛奶中四环素类药物残留的方法
EP3856923A1 (en) * 2018-09-28 2021-08-04 Ipsen Biopharm Limited Cell-based clostridal neurotoxin assays
CN109211863B (zh) * 2018-10-26 2021-04-20 大连民族大学 利用Eu2+f-f跃迁光谱检测爆炸物TNP的方法
TW202113072A (zh) * 2019-06-07 2021-04-01 美商辛那皮克研究有限責任公司 基因工程化細胞
US20210373021A1 (en) * 2020-06-01 2021-12-02 Galderma Holding S.A. Snap-25 reporter constructs and methods of using the same
CN112557353B (zh) * 2020-12-17 2022-02-18 天津工业大学 一种基于延迟发光光谱的细胞活力检测方法和装置
WO2024107023A1 (ko) * 2022-11-18 2024-05-23 주식회사 바이오링크스 재조합 형광단백질을 이용한 세포 기반 보툴리눔 독소의 검출 또는 활성 측정 방법

Family Cites Families (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5804604A (en) 1989-12-21 1998-09-08 Biogen, Inc. Tat-derived transport polypeptides and fusion proteins
AU658818B2 (en) * 1989-12-21 1995-05-04 Johns Hopkins University School Of Medicine, The Method of delivering molecules into eukaryotic cells using tat protein conjugate
GB9411138D0 (en) * 1994-06-03 1994-07-27 Microbiological Res Authority Toxin assay
US5962637A (en) 1994-06-03 1999-10-05 Microbiological Research Authority Toxin assay
GB9508204D0 (en) 1995-04-21 1995-06-07 Speywood Lab Ltd A novel agent able to modify peripheral afferent function
US6069010A (en) 1995-09-11 2000-05-30 Axys Pharmaceuticals, Inc. High throughput gene inactivation with large scale gene targeting
US6803188B1 (en) 1996-01-31 2004-10-12 The Regents Of The University Of California Tandem fluorescent protein constructs
US5965699A (en) * 1996-11-06 1999-10-12 The United States Of America As Represented By The Secretary Of The Army Assay for the proteolytic activity of serotype a from clostridium botulinum
US6221355B1 (en) 1997-12-10 2001-04-24 Washington University Anti-pathogen system and methods of use thereof
AU3665899A (en) 1998-04-28 1999-11-16 Washington University Methods for transducing fusion molecules
US7090976B2 (en) * 1999-11-10 2006-08-15 Rigel Pharmaceuticals, Inc. Methods and compositions comprising Renilla GFP
JP3863372B2 (ja) 1998-10-30 2006-12-27 セロミックス インコーポレイテッド プロテアーゼバイオセンサーをコードする組換え核酸、組換え発現ベクター、プロテアーゼバイオセンサー、化合物を同定する方法、及び化合物を同定するためのキット
DE19856897A1 (de) * 1998-12-10 2000-06-15 Biotecon Ges Fuer Biotechnologische Entwicklung & Consulting Mbh Therapeutikum zur Unterdrückung von Schnarchgeräuschen
WO2000034308A2 (en) 1998-12-10 2000-06-15 Washington University Protein transduction system and methods of use thereof
AU2001262906A1 (en) 2000-02-11 2001-08-20 Cellomics, Inc. Peptide biosensors for anthrax protease
US6325899B1 (en) 2000-03-10 2001-12-04 Action Caps, Llc Disposable and recyclable intermediates for use in electrostatic coating processes
FR2809733B1 (fr) * 2000-06-02 2004-04-30 Inst Nat Sante Rech Med Substrat peptidique reconnu par la toxine botulique de type bont/b et son utilisation pour doser et/ou detecter ladite toxine ou des inhibiteurs correspondants
DE60131468D1 (de) 2000-09-25 2007-12-27 U S Medical Res Inst Of Infect Hoch-durchsatz assays für proteolytische aktivitäten von clostridium neurotoxinen
AU2002217839A1 (en) * 2000-11-22 2002-06-03 The Regents Of The University Of California A method to measure the activation state of signaling pathways in cells
EP1267171A1 (en) * 2001-06-14 2002-12-18 Institut National De La Sante Et De La Recherche Medicale (Inserm) Cofactor-based screening method for nuclear receptor modulators
US7332567B2 (en) 2001-08-28 2008-02-19 Allergan, Inc. Fret protease assays for clostridial toxins
US7208285B2 (en) 2001-08-28 2007-04-24 Allergan, Inc. Fret protease assays for botulinum serotype A/E toxins
US7374896B2 (en) 2001-08-28 2008-05-20 Allergan, Inc. GFP-SNAP25 fluorescence release assay for botulinum neurotoxin protease activity
US20080064054A1 (en) 2001-08-28 2008-03-13 Ester Fernandez-Salas Fluorescence resonance energy transfer (fret) assays for clostridial toxin activity
US8022172B2 (en) 2001-08-28 2011-09-20 Allergan, Inc. Luminescence resonance energy transfer (LRET) assays for clostridial toxin activity
US7183066B2 (en) 2002-09-27 2007-02-27 Allergan, Inc. Cell-based fluorescence resonance energy transfer (FRET) assays for clostridial toxins
WO2005082096A2 (en) 2004-02-24 2005-09-09 Allergan, Inc. Botulinum toxin screening assays
US7399607B2 (en) 2004-09-22 2008-07-15 Allergan, Inc. Fluorescence polarization assays for determining clostridial toxin activity
ES2345506T3 (es) 2005-04-05 2010-09-24 Allergan, Inc. Ensayos de fret a base de colorantes lipofilos para determinar la actividad de la toxina clostridial.
ATE456798T1 (de) 2005-10-12 2010-02-15 Allergan Inc Tests der molekularen oder subzellulären interaktivität unter verwendung von depolarisierung nach resonanzenergietransfer (daret)

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