BRPI0514318A - ensaio de liberação de fluorescência com relação à atividade de protease da toxina do botulismo em gfp-snap25 - Google Patents
ensaio de liberação de fluorescência com relação à atividade de protease da toxina do botulismo em gfp-snap25Info
- Publication number
- BRPI0514318A BRPI0514318A BRPI0514318-7A BRPI0514318A BRPI0514318A BR PI0514318 A BRPI0514318 A BR PI0514318A BR PI0514318 A BRPI0514318 A BR PI0514318A BR PI0514318 A BRPI0514318 A BR PI0514318A
- Authority
- BR
- Brazil
- Prior art keywords
- snap25
- gfp
- fluorescent protein
- partner
- cleavage site
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/001—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof by chemical synthesis
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
- C07K14/43595—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from coelenteratae, e.g. medusae
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
- C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/24—Metalloendopeptidases (3.4.24)
- C12Y304/24069—Bontoxilysin (3.4.24.69), i.e. botulinum neurotoxin
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56911—Bacteria
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
- G01N2333/33—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Clostridium (G)
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicinal Chemistry (AREA)
- Wood Science & Technology (AREA)
- Immunology (AREA)
- Biophysics (AREA)
- Toxicology (AREA)
- Gastroenterology & Hepatology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Urology & Nephrology (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Cell Biology (AREA)
- Hematology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Food Science & Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
ENSAIO DE LIBERAçãO DE FLUORESCêNCIA COM RELAçãO à ATIVIDADE DE PROTEASE DA TOXINA DO BOTULISMO EM GFP-SNAP25. A presente invenção refere-se a uma molécula de ácido nucléico que contém uma seqüência de nucleotídeo que codifica um substrato SNAP-25 que inclui (1) uma proteína fluorescente verde; (ii) um primeiro parceiro de uma dupla de afinidade; e (iii) uma porção de SNAP-25 que inclui uma seqüência de reconhecimento BoNT/A, BoNT/C1 ou BoNT/E contendo um sítio de clivagem, em que o sitio de clivagem intervém entre a proteína fluorescente verde e o primeiro parceiro da dupla de afinidade. Também é provido aqui, neste pedido de patente, uma molécula de ácido nucléico que contêm uma seqüência de nucleotídeo que codifica um substrato de toxina alvo que inclui (1) uma proteína fluorescente, (ii) um primeiro parceiro de uma dupla de afinidade; e (iii) uma seqüência de reconhecimento da toxina do clostrídico contendo um sítio de clivagem, em que o sitio de clivagem intervém entre a proteína fluorescente e o primeiro parceiro da dupla de afinidade.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US10/917,844 US7374896B2 (en) | 2001-08-28 | 2004-08-13 | GFP-SNAP25 fluorescence release assay for botulinum neurotoxin protease activity |
PCT/US2005/028497 WO2006020748A2 (en) | 2004-08-13 | 2005-08-10 | Gfp-snap25 fluorescence release assay for botulinum toxin protease activity |
Publications (1)
Publication Number | Publication Date |
---|---|
BRPI0514318A true BRPI0514318A (pt) | 2008-06-10 |
Family
ID=35908131
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
BRPI0514318-7A BRPI0514318A (pt) | 2004-08-13 | 2005-08-10 | ensaio de liberação de fluorescência com relação à atividade de protease da toxina do botulismo em gfp-snap25 |
Country Status (7)
Country | Link |
---|---|
US (5) | US7374896B2 (pt) |
EP (1) | EP1776380A2 (pt) |
JP (1) | JP2008509667A (pt) |
AU (1) | AU2005272796A1 (pt) |
BR (1) | BRPI0514318A (pt) |
CA (1) | CA2576354A1 (pt) |
WO (1) | WO2006020748A2 (pt) |
Families Citing this family (47)
Publication number | Priority date | Publication date | Assignee | Title |
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US7332567B2 (en) * | 2001-08-28 | 2008-02-19 | Allergan, Inc. | Fret protease assays for clostridial toxins |
US20080064054A1 (en) * | 2001-08-28 | 2008-03-13 | Ester Fernandez-Salas | Fluorescence resonance energy transfer (fret) assays for clostridial toxin activity |
US7374896B2 (en) * | 2001-08-28 | 2008-05-20 | Allergan, Inc. | GFP-SNAP25 fluorescence release assay for botulinum neurotoxin protease activity |
US7208285B2 (en) * | 2001-08-28 | 2007-04-24 | Allergan, Inc. | Fret protease assays for botulinum serotype A/E toxins |
US8022172B2 (en) * | 2001-08-28 | 2011-09-20 | Allergan, Inc. | Luminescence resonance energy transfer (LRET) assays for clostridial toxin activity |
US20040042961A1 (en) * | 2002-07-31 | 2004-03-04 | Robert Menard | Development of an in vivo functional assay for proteases |
US7183066B2 (en) * | 2002-09-27 | 2007-02-27 | Allergan, Inc. | Cell-based fluorescence resonance energy transfer (FRET) assays for clostridial toxins |
AU2003272800A1 (en) * | 2002-10-01 | 2004-04-23 | University Of Maryland | Methods for identifying inhibitors of botulinum neurotoxins |
US8137924B2 (en) | 2003-12-19 | 2012-03-20 | Wisconsin Alumni Research Foundation | Method and compositions for detecting botulinum neurotoxin |
US7399607B2 (en) * | 2004-09-22 | 2008-07-15 | Allergan, Inc. | Fluorescence polarization assays for determining clostridial toxin activity |
ES2351942T3 (es) * | 2005-04-05 | 2011-02-14 | Allergan Inc | Analisis de la actividad de una toxina clostridial. |
ES2345506T3 (es) | 2005-04-05 | 2010-09-24 | Allergan, Inc. | Ensayos de fret a base de colorantes lipofilos para determinar la actividad de la toxina clostridial. |
CA2610103A1 (en) * | 2005-09-19 | 2007-03-19 | Allergan, Inc. | Clostridial toxin activatable clostridial toxins |
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CA2699612C (en) * | 2007-09-14 | 2013-01-08 | Biosentinel, Llc | Resonance energy transfer assay with cleavage sequence and spacer |
ES2524312T3 (es) | 2008-03-14 | 2014-12-05 | Allergan, Inc. | Ensayos de actividad de serotipo A de toxina botulínica de base inmunológica |
KR101604515B1 (ko) * | 2008-03-14 | 2016-03-17 | 알러간, 인코포레이티드 | 면역-기반 보툴리눔 독소 세로타입 a 활성 검정 |
AU2009286973B2 (en) | 2008-08-29 | 2014-06-12 | Merz Pharma Gmbh & Co. Kgaa | Clostridial neurotoxins with altered persistency |
KR20110106346A (ko) | 2008-12-10 | 2011-09-28 | 알러간, 인코포레이티드 | 클로스트리디움 독소 약제학적 조성물 |
SG174353A1 (en) * | 2009-03-13 | 2011-11-28 | Allergan Inc | Immuno-based retargeted endopeptidase activity assays |
AU2011348204B2 (en) | 2010-12-22 | 2017-03-02 | President And Fellows Of Harvard College | Continuous directed evolution |
EP2578695A1 (en) * | 2011-10-05 | 2013-04-10 | Albert-Ludwigs-Universität Freiburg | Assay for Clostridium botulinum Neurotoxin |
WO2013102088A2 (en) | 2011-12-31 | 2013-07-04 | Allergan, Inc. | Highly Sensitive Cell-Based Assay to Detect the Presence of Active Botulinum Neurotoxin Serotype-A |
GB201219602D0 (en) | 2012-10-31 | 2012-12-12 | Syntaxin Ltd | Recombinant clostridium botulinum neurotoxins |
US8962340B2 (en) | 2012-12-03 | 2015-02-24 | Src, Inc. | Real-time assay for the detection of botulinum toxin |
PL2952205T3 (pl) | 2014-06-06 | 2018-01-31 | Kleiner Fisman Galit | Toksyna botulinowa do zastosowania do leczenia paratonii |
US10571477B2 (en) * | 2014-07-07 | 2020-02-25 | Protagen Gmbh | Marker sequences for diagnosing and stratifying systemic sclerosis patients |
US10920208B2 (en) | 2014-10-22 | 2021-02-16 | President And Fellows Of Harvard College | Evolution of proteases |
CN107003310B (zh) * | 2014-11-21 | 2019-07-19 | 莫茨药物股份两合公司 | 用于测定神经毒素多肽的生物活性的方法 |
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US10392674B2 (en) | 2015-07-22 | 2019-08-27 | President And Fellows Of Harvard College | Evolution of site-specific recombinases |
US11524983B2 (en) | 2015-07-23 | 2022-12-13 | President And Fellows Of Harvard College | Evolution of Bt toxins |
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TWI737742B (zh) | 2016-06-22 | 2021-09-01 | 德商梅茲製藥有限兩合公司 | 肉毒桿菌毒素的預填充式注射器系統、具有其之套組以及其之使用 |
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CN116693638B (zh) * | 2023-05-15 | 2024-03-08 | 西北农林科技大学 | Pg1-lc蛋白作为snap-25的水解酶的应用 |
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2004
- 2004-08-13 US US10/917,844 patent/US7374896B2/en not_active Expired - Lifetime
-
2005
- 2005-08-10 BR BRPI0514318-7A patent/BRPI0514318A/pt not_active Application Discontinuation
- 2005-08-10 JP JP2007525782A patent/JP2008509667A/ja active Pending
- 2005-08-10 AU AU2005272796A patent/AU2005272796A1/en not_active Abandoned
- 2005-08-10 EP EP05826811A patent/EP1776380A2/en not_active Withdrawn
- 2005-08-10 CA CA002576354A patent/CA2576354A1/en not_active Abandoned
- 2005-08-10 WO PCT/US2005/028497 patent/WO2006020748A2/en active Application Filing
-
2008
- 2008-01-23 US US12/018,637 patent/US20080166739A1/en not_active Abandoned
- 2008-01-24 US US12/019,188 patent/US20080171348A1/en not_active Abandoned
- 2008-01-24 US US12/019,070 patent/US20080176249A1/en not_active Abandoned
- 2008-01-24 US US12/019,120 patent/US7838260B2/en not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
US20060154314A9 (en) | 2006-07-13 |
US7838260B2 (en) | 2010-11-23 |
CA2576354A1 (en) | 2006-03-23 |
US7374896B2 (en) | 2008-05-20 |
US20080176249A1 (en) | 2008-07-24 |
JP2008509667A (ja) | 2008-04-03 |
AU2005272796A1 (en) | 2006-02-23 |
US20080213796A1 (en) | 2008-09-04 |
EP1776380A2 (en) | 2007-04-25 |
WO2006020748A2 (en) | 2006-02-23 |
US20050100973A1 (en) | 2005-05-12 |
WO2006020748A3 (en) | 2006-10-05 |
US20080171348A1 (en) | 2008-07-17 |
US20080166739A1 (en) | 2008-07-10 |
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