NO178076C - Fremgangsmåte til å fremstille heterologe proteiner i Pichia pastoris ved bruk av ekspresjonsvektorer inneholdende sekvenser som induseres av metanol - Google Patents

Fremgangsmåte til å fremstille heterologe proteiner i Pichia pastoris ved bruk av ekspresjonsvektorer inneholdende sekvenser som induseres av metanol

Info

Publication number
NO178076C
NO178076C NO854312A NO854312A NO178076C NO 178076 C NO178076 C NO 178076C NO 854312 A NO854312 A NO 854312A NO 854312 A NO854312 A NO 854312A NO 178076 C NO178076 C NO 178076C
Authority
NO
Norway
Prior art keywords
methanol
expression vectors
pichia pastoris
vectors containing
heterologous proteins
Prior art date
Application number
NO854312A
Other languages
English (en)
Norwegian (no)
Other versions
NO178076B (no
NO854312L (no
Inventor
David Womack Stroman
Paul Frederick Brust
Steven Bradley Ellis
Thomas Raymond Gingeras
Michael Miller Harpold
Juerg Friedrich Tschopp
Original Assignee
Res Corp Technologies Inc
Phillips Petroleum Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US06/666,391 external-priority patent/US4808537A/en
Application filed by Res Corp Technologies Inc, Phillips Petroleum Co filed Critical Res Corp Technologies Inc
Publication of NO854312L publication Critical patent/NO854312L/no
Publication of NO178076B publication Critical patent/NO178076B/no
Publication of NO178076C publication Critical patent/NO178076C/no

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C12N15/68—Stabilisation of the vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Saccharide Compounds (AREA)
NO854312A 1984-10-30 1985-10-29 Fremgangsmåte til å fremstille heterologe proteiner i Pichia pastoris ved bruk av ekspresjonsvektorer inneholdende sekvenser som induseres av metanol NO178076C (no)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US06/666,391 US4808537A (en) 1984-10-30 1984-10-30 Methanol inducible genes obtained from pichia and methods of use
US06/780,102 US4855231A (en) 1984-10-30 1985-09-25 Regulatory region for heterologous gene expression in yeast

Publications (3)

Publication Number Publication Date
NO854312L NO854312L (no) 1986-05-02
NO178076B NO178076B (no) 1995-10-09
NO178076C true NO178076C (no) 1996-01-17

Family

ID=27099454

Family Applications (1)

Application Number Title Priority Date Filing Date
NO854312A NO178076C (no) 1984-10-30 1985-10-29 Fremgangsmåte til å fremstille heterologe proteiner i Pichia pastoris ved bruk av ekspresjonsvektorer inneholdende sekvenser som induseres av metanol

Country Status (23)

Country Link
US (1) US4855231A (fr)
EP (2) EP0183071B1 (fr)
JP (5) JP2502508B2 (fr)
KR (1) KR930001117B1 (fr)
CN (1) CN1011243B (fr)
AR (1) AR242988A1 (fr)
AT (1) ATE83263T1 (fr)
AU (1) AU572002B2 (fr)
CA (1) CA1340733C (fr)
DD (1) DD254211A5 (fr)
DE (1) DE3586889T2 (fr)
DK (1) DK496385A (fr)
ES (1) ES8609458A1 (fr)
FI (1) FI94427C (fr)
GR (1) GR852611B (fr)
HU (1) HU205627B (fr)
IE (1) IE930804L (fr)
IL (1) IL76765A (fr)
NO (1) NO178076C (fr)
NZ (1) NZ213842A (fr)
PL (1) PL158965B1 (fr)
PT (1) PT81399B (fr)
YU (1) YU46695B (fr)

Families Citing this family (115)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5741672A (en) * 1984-07-27 1998-04-21 Unilever Patent Holdings B.V. Expression and production of polypeptides using the promoters of the hansenula polymorpha MOX and DAS genes
US4879231A (en) * 1984-10-30 1989-11-07 Phillips Petroleum Company Transformation of yeasts of the genus pichia
US5032516A (en) * 1985-10-25 1991-07-16 Phillips Petroleum Company Pichia pastoris alcohol oxidase II regulatory region
US4882279A (en) * 1985-10-25 1989-11-21 Phillips Petroleum Company Site selective genomic modification of yeast of the genus pichia
US4818700A (en) * 1985-10-25 1989-04-04 Phillips Petroleum Company Pichia pastoris argininosuccinate lyase gene and uses thereof
US4895800A (en) * 1985-11-26 1990-01-23 Phillips Petroleum Company Yeast production of hepatitis B surface antigen
IL81817A0 (en) * 1986-03-14 1987-10-20 Phillips Petroleum Co Methanol and glucose responsive yeast regulatory regions
ZA872534B (en) * 1986-05-08 1987-11-25 Phillips Petroleum Co Yeast production of streptokinase
IL82935A0 (en) * 1986-08-12 1987-12-20 Phillips Petroleum Co Secretion of heterologous proteins from yeast
US5002876A (en) * 1986-09-22 1991-03-26 Phillips Petroleum Company Yeast production of human tumor necrosis factor
DE3789866T2 (de) * 1987-07-17 1994-09-22 Rhein Biotech Proz & Prod Gmbh DNA-Moleküle, die für FMDH-Kontrollabschnitte und Strukturgene für ein Protein mit FMDH-Aktivität kodieren, sowie deren Anwendung.
ES2204884T3 (es) * 1988-01-05 2004-05-01 Roche Diagnostics Gmbh Procedimiento para la obtencion de proteinas o productos genicos que contienen proteinas.
FI891695A (fi) * 1988-04-11 1989-10-12 Phillips Petroleum Co Expression av laxtillvaexthormon i methyltrofisk jaest av slaektet pichia.
IL90021A0 (en) * 1988-04-25 1989-12-15 Phillips Petroleum Co Process for the production of interferon
NZ228774A (en) * 1988-04-25 1991-05-28 Phillips Petroleum Co Hbv particles containing pres 2 proteins and recombinant processes for their production in yeast cells
IL89992A0 (en) 1988-04-25 1989-12-15 Phillips Petroleum Co Expression of human serum albumin in methylotrophic yeasts
IL89993A0 (en) * 1988-04-25 1989-12-15 Phillips Petroleum Co Expression of the hiv 24kda gag protein in methylotrophic yeasts
IL89989A0 (en) * 1988-04-25 1989-12-15 Phillips Petroleum Co Expression of human interleukin-2 in methylotrophic yeasts
DE3851535T2 (de) * 1988-12-20 1995-02-02 Rhein Biotech Proz & Prod Gmbh Mutanter Stamm von einem methylotrophen Organismus und Verfahren zur Herstellung eines Proteins in methylotrophen Organismus.
AU5196290A (en) * 1989-02-13 1990-09-05 Salk Institute Biotechnology/Industrial Associates, Inc., The Production of superoxide dismutase in pichia pastoris yeast cells
GB8924883D0 (en) * 1989-11-03 1989-12-20 Shell Int Research Expression vector and polypeptide synthesis
US5264554A (en) * 1990-01-19 1993-11-23 The Blood Center Of Southeastern Wisconsin, Inc. Platelet cell adhesion molecule and variants thereof
US5369028A (en) * 1990-04-03 1994-11-29 The Salk Institute Biotechnology/Industrial Associates, Inc. DNA and mRNA encoding human neuronal nicotinic acetylcholine receptor compositions and cells transformed with same
US6440681B1 (en) 1990-04-03 2002-08-27 Merck & Co., Inc. Methods for identifying agonists and antagonists for human neuronal nicotinic acetylcholine receptors
US5258302A (en) * 1990-07-03 1993-11-02 The Salk Institute Biotechnology/Industrial Associates, Inc. DNA for expression of aprotinin in methylotrophic yeast cells
US5612198A (en) * 1990-09-04 1997-03-18 The Salk Institute Production of insulin-like growth factor-1 in methylotrophic yeast cells
CA2090969C (fr) * 1990-09-04 2001-08-21 Russell Arthur Brierley Production de facteur de croissance insulinoide dans les cellules de levure methylotrophiques
US5268273A (en) * 1990-12-14 1993-12-07 Phillips Petroleum Company Pichia pastoris acid phosphatase gene, gene regions, signal sequence and expression vectors comprising same
WO1992013951A1 (fr) * 1991-02-04 1992-08-20 The Salk Institute Biotechnology/Industrial Associates, Inc. Production de serumalbumine humaine dans des cellules de levure methylotrophique
EP0578746B1 (fr) * 1991-04-01 2002-07-03 Merck & Co., Inc. GENES INFLUAN ANT L'ACTIVITE PROTEOLYTIQUE DE $i(PICHIA), ET LEURS UTILISATIONS
CA2058820C (fr) * 1991-04-25 2003-07-15 Kotikanyad Sreekrishna Cassettes d'expression et vecteurs pour la secretion de serum albumine humaine dans des cellules pichia pastoris
US5330901A (en) * 1991-04-26 1994-07-19 Research Corporation Technologies, Inc. Expression of human serum albumin in Pichia pastoris
US5834262A (en) * 1992-01-06 1998-11-10 E. I. Du Pont De Nemours And Company Oxidation of glycolic acid to glyoxylic acid using a microbial cell transformant as catalyst
ES2093294T3 (es) * 1992-02-28 1996-12-16 Suntory Ltd Nuevo vector que comprende un promotor inducible por el metanol y/o glicerol.
US5708141A (en) * 1992-05-11 1998-01-13 Corvas International, Inc. Neutrophil inhibitors
AU6355494A (en) * 1993-03-03 1994-09-26 E.I. Du Pont De Nemours And Company Production of glycolate oxidase in methylotrophic yeast
CA2155330C (fr) 1993-03-08 2002-01-29 Kathryn J. Elliott Compositions pour le recepteur nicotinique neuronal humain de l'acetylcholine; methode d'utilisation
WO1994021802A1 (fr) * 1993-03-25 1994-09-29 Biosource Genetics Corporation CLONES GENOMIQUES ZZA1 et ZZA2 D'OXYDASE D'ALCOOL $i(PICHIA PASTORIS)
ES2182843T3 (es) 1993-04-20 2003-03-16 Merck & Co Inc Subunidades de receptor humano de n-metil-d-aspartato, acidos nucleicos que codifican las mismas y su uso.
US5912122A (en) 1993-06-04 1999-06-15 Sibia Neurosciences, Inc. Nucleic acids encoding and method for detecting nucleic acid encoding human metabotropic glutamate receptor subtype mGluR6
US5521297A (en) 1993-06-04 1996-05-28 Salk Institute Biotechnology/Industrial Associates Nucleic acids encoding human metabotropic glutamate receptors
US6001581A (en) * 1993-06-04 1999-12-14 Sibia Neurosciences, Inc. Cation-based bioassay using human metabotropic glutamate receptors
US6638905B2 (en) 1993-06-18 2003-10-28 The Salk Institute For Biological Studies Cloning and recombinant production of CFR receptor(s)
US6495343B1 (en) 1993-06-18 2002-12-17 The Salk Institute For Biological Studies Cloning and recombinant production of CRF receptor(s)
AU1091595A (en) * 1993-11-08 1995-05-29 Salk Institute Biotechnology/Industrial Associates, Inc., The Human neuronal nicotinic acetylcholine receptor compositions and methods employing same
US5453161A (en) * 1993-11-12 1995-09-26 Enichem S.P.A. Polyamic acid to polyimide conversion by microwave heating
US5866542A (en) * 1994-10-18 1999-02-02 Corvas International, Inc. Nematode-extracted anticoagulant protein
CA2202351A1 (fr) * 1994-10-18 1996-04-25 George Phillip Vlasuk Proteines anticoagulantes et inhibiteurs de la serine-protease extraits de nematodes
US5866543A (en) * 1995-06-05 1999-02-02 Corvas International, Inc. Nematode-extracted anticoagulant protein
US5945275A (en) * 1994-10-18 1999-08-31 Corvas International, Inc. Nematode-extracted anticoagulant protein
US5872098A (en) * 1995-06-05 1999-02-16 Corvas International, Inc. Nematode-extracted anticoagulant protein
JP3811186B2 (ja) * 1994-10-18 2006-08-16 コーバス インターナショナル， インコーポレイテッド 線虫から抽出されるセリンプロテアーゼ阻害剤および抗凝固性タンパク質
US5863894A (en) * 1995-06-05 1999-01-26 Corvas International, Inc. Nematode-extracted anticoagulant protein
US6720140B1 (en) * 1995-06-07 2004-04-13 Invitrogen Corporation Recombinational cloning using engineered recombination sites
US6143557A (en) * 1995-06-07 2000-11-07 Life Technologies, Inc. Recombination cloning using engineered recombination sites
US6485967B1 (en) 1995-06-07 2002-11-26 Merck & Co., Inc. Human neuronal nicotinic acetylcholine receptor α6 and β3 nucleic acid
US5965389A (en) * 1995-11-09 1999-10-12 Zymogenetics, Inc. Production of GAD65 in methylotrophic yeast
MX9605082A (es) 1996-10-24 1998-04-30 Univ Autonoma De Nuevo Leon Levaduras metilotroficas modificadas geneticamente para la produccion y secrecion de hormona de crecimiento humano.
DE69834533T2 (de) 1997-10-01 2006-10-12 Dsm Ip Assets B.V. Verfahren zur Protein-Herstellung
US7351578B2 (en) * 1999-12-10 2008-04-01 Invitrogen Corp. Use of multiple recombination sites with unique specificity in recombinational cloning
US20030124555A1 (en) * 2001-05-21 2003-07-03 Invitrogen Corporation Compositions and methods for use in isolation of nucleic acid molecules
WO1999021977A1 (fr) * 1997-10-24 1999-05-06 Life Technologies, Inc. Clonage recombinatoire au moyen d'acides nucleiques possedant des sites de recombinaison
CN101125873A (zh) * 1997-10-24 2008-02-20 茵维特罗根公司 利用具重组位点的核酸进行重组克隆
DE69941406D1 (de) * 1998-07-06 2009-10-22 Tosoh Corp IL-6 Rezeptor IL-6-gekoppeltes Fusionsprotein
AU5584999A (en) * 1998-08-28 2000-03-21 Invitrogen Corporation System for the rapid manipulation of nucleic acid sequences
US6780615B1 (en) 1998-12-31 2004-08-24 Genway Biotech Inc. Production of recombinant monellin using methylotrophic yeast expression system
US6720174B1 (en) 1999-01-28 2004-04-13 Novozymes A/S Phytases
ATE443074T1 (de) 1999-03-02 2009-10-15 Life Technologies Corp Zubereitungen und methoden zur verwendung in rekombinatorischem klonen von nukleinsäuren
US7504253B2 (en) * 1999-06-11 2009-03-17 The Burnham Institute For Medical Research Nucleic acid encoding proteins involved in protein degradation, products and methods related thereof
US6261820B1 (en) * 1999-10-01 2001-07-17 Amgen Inc. Fibronolytically active polypeptide
US6440414B1 (en) * 1999-10-01 2002-08-27 Amgen Inc. Pharmaceutical compositions of fibrinolytic agent
MXPA02004666A (es) 1999-11-12 2003-06-24 Fibrogen Inc Gelatinas recombinantes.
CA2392753A1 (fr) 1999-12-10 2001-06-14 Invitrogen Corporation Utilisation de sites de recombinaison multiples avec une specificite unique dans le clonage de recombinaison
US7033776B2 (en) * 1999-12-17 2006-04-25 Amgen Inc. Method for treatment of indwelling catheter occlusion using fibrinolytic metalloproteinases
WO2001077351A1 (fr) * 2000-04-07 2001-10-18 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Vecteurs et procedes d'expression de proteine double dans pichia pastoris et escherichia coli
US7244560B2 (en) * 2000-05-21 2007-07-17 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
US7863020B2 (en) * 2000-06-28 2011-01-04 Glycofi, Inc. Production of sialylated N-glycans in lower eukaryotes
US8697394B2 (en) * 2000-06-28 2014-04-15 Glycofi, Inc. Production of modified glycoproteins having multiple antennary structures
US7598055B2 (en) * 2000-06-28 2009-10-06 Glycofi, Inc. N-acetylglucosaminyltransferase III expression in lower eukaryotes
WO2002000879A2 (fr) 2000-06-28 2002-01-03 Glycofi, Inc. Procede de production de glycoproteines modifiees
US7449308B2 (en) * 2000-06-28 2008-11-11 Glycofi, Inc. Combinatorial DNA library for producing modified N-glycans in lower eukaryotes
US7625756B2 (en) 2000-06-28 2009-12-01 GycoFi, Inc. Expression of class 2 mannosidase and class III mannosidase in lower eukaryotic cells
US6803225B2 (en) 2000-06-30 2004-10-12 Flanders Interuniversity Institute For Biotechnology Protein glycosylation modification in Pichia pastoris
US7009045B2 (en) * 2000-07-14 2006-03-07 Archer-Daniels-Midland Company Transformation systems for flavinogenic yeast
US7198924B2 (en) * 2000-12-11 2007-04-03 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
EP1385866A4 (fr) 2001-04-05 2006-03-29 Univ Nebraska Sequences nucleotidiques regulatrices d'alcool oxydase 1 pour l'expression de genes heterologues dans une levure
US6645739B2 (en) 2001-07-26 2003-11-11 Phoenix Pharmacologies, Inc. Yeast expression systems, methods of producing polypeptides in yeast, and compositions relating to same
EP1497434A4 (fr) * 2002-04-15 2005-08-24 Merck & Co Inc Analyse matricielle d'expression genique dans des cellules
US7252933B2 (en) 2002-06-26 2007-08-07 Flanders Interuniversity Institute For Biotechnology Protein glycosylation modification in methylotrophic yeast
US20040204953A1 (en) * 2002-07-17 2004-10-14 Lincoln Muir Subscription based systems, methods and components for providing genomic and proteomic products and services
DE60335602D1 (de) * 2002-12-18 2011-02-17 Roche Diagnostics Gmbh Rekombinante Desoxyribonuclease I aus Rinder-Pankreas mit hoher spezifischer Aktivität
ATE387494T1 (de) * 2002-12-20 2008-03-15 Hoffmann La Roche Hitzelabile desoxyribonuklease i-varianten
US7332299B2 (en) 2003-02-20 2008-02-19 Glycofi, Inc. Endomannosidases in the modification of glycoproteins in eukaryotes
EP1460425A1 (fr) * 2003-03-17 2004-09-22 Boehringer Mannheim Gmbh Enzymes déglycosylés pour conjugués
EP1673457B1 (fr) * 2003-08-25 2011-05-18 Funzyme Biotechnologies SA Nouvelles proteines fongiques et acides nucleiques codant pour celles-ci
US8293503B2 (en) 2003-10-03 2012-10-23 Promega Corporation Vectors for directional cloning
EP1697534B1 (fr) 2003-12-01 2010-06-02 Life Technologies Corporation Molecule d'acide nucleique contenant des sites de recombinaison et leurs procedes d'utilisation
DE602004025192D1 (de) * 2003-12-05 2010-03-11 Roche Diagnostics Gmbh Rekombinante Carboxypeptidase B und ihre Aufreinigung
ATE517919T1 (de) * 2005-09-14 2011-08-15 Sanofi Aventis Deutschland Spaltung von insulinvorstufen durch eine trypsinvariante
AT504588B1 (de) * 2006-11-16 2008-08-15 Univ Graz Tech Nukleinsäure-promotor
ES2531194T3 (es) 2008-03-03 2015-03-11 Abbvie Inc Métodos para transformar levaduras
KR20110101198A (ko) 2008-12-12 2011-09-15 이 아이 듀폰 디 네모아 앤드 캄파니 재생 가능 자원으로부터의 선형 다이카르복실산의 제조 방법
EP2258855A1 (fr) 2009-05-28 2010-12-08 Universität für Bodenkultur Wien Séquences d'expression
CA2784944A1 (fr) 2009-12-21 2011-06-30 Centre Hospitalier Universitaire Vaudois Action synergique d'une prolyl protease et de tripeptidyl proteases
SG194937A1 (en) 2011-05-20 2013-12-30 Alderbio Holdings Llc High-purity production of multi-subunit proteins such as antibodies in transformed microbes such as pichia pastoris
BR112014003841B1 (pt) 2011-08-19 2022-02-15 H. Lundbeck A/S Método para identificar uma célula hospedeira diploide de pichia pastoris que produz um anticorpo desejado com uma maior pureza
KR102128957B1 (ko) 2011-10-07 2020-07-02 론자 리미티드 조절가능한 프로모터
TWI670279B (zh) 2013-03-15 2019-09-01 美商艾爾德生物製藥股份有限公司 抗體純化及純度監測
CA2905180C (fr) 2013-03-15 2023-03-07 Alder Biopharmaceuticals, Inc. Changement de temperature pour augmenter le rendement de l'expression de polypeptides dans la levure et d'autres cellules transformees
US9150870B2 (en) 2013-03-15 2015-10-06 Lonza Ltd. Constitutive promoter
WO2014139608A1 (fr) 2013-03-15 2014-09-18 Lonza Ltd Promoteur constitutif
US10053501B2 (en) 2013-03-21 2018-08-21 Commonwealth Scientific And Industrial Research Organisation Purification of triple helical proteins
JP6440687B2 (ja) 2013-04-30 2018-12-19 ドナルド ダンフォース プラント サイエンス センター 抗真菌性植物タンパク質、ペプチド、及び使用方法
KR102384622B1 (ko) 2013-09-09 2022-04-11 에보닉 오퍼레이션스 게엠베하 변형된 세균 콜라겐-유사 단백질
WO2016120764A1 (fr) 2015-01-26 2016-08-04 Centre Hospitalier Universitaire Vaudois (Chuv) Prolyl-endopeptidases d'aspergillus oryzae et utilisation associée dans la dégradation de polypeptides

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IE54046B1 (en) * 1981-08-25 1989-05-24 Celltech Ltd Expression vectors
IE54592B1 (en) * 1982-03-08 1989-12-06 Genentech Inc Anumal interferons, processes involved in their production, compositions containing them, dna sequences coding therefor and espression vehicles containing such sequences and cells transformed thereby
PT76727B (en) * 1982-05-19 1985-12-27 Gist Brocades Nv Process for the preparation of clones for kluyveromyces species
WO1984001153A1 (fr) * 1982-09-15 1984-03-29 Collaborative Res Inc Production d'interferon dans une levure par l'utilisation d'un promoteur d'invertase
EP0173378B1 (fr) * 1984-07-27 1991-06-12 Unilever N.V. Procédé de préparation d'un polypeptide par culture des micro-organismes transformés, micro-organisme correspondant et séquences d'ADN utiles pour la préparation d'un tel micro-organisme.
US4837148A (en) * 1984-10-30 1989-06-06 Phillips Petroleum Company Autonomous replication sequences for yeast strains of the genus pichia
US4879231A (en) * 1984-10-30 1989-11-07 Phillips Petroleum Company Transformation of yeasts of the genus pichia
US4885242A (en) * 1984-10-30 1989-12-05 Phillips Petroleum Company Genes from pichia histidine pathway and uses thereof

Also Published As

Publication number Publication date
YU46695B (sh) 1994-04-05
CN85109718A (zh) 1986-08-20
JPH08173161A (ja) 1996-07-09
AU572002B2 (en) 1988-04-28
NO178076B (no) 1995-10-09
PL256012A1 (en) 1986-09-23
JPH0698789A (ja) 1994-04-12
US4855231A (en) 1989-08-08
EP0183071A2 (fr) 1986-06-04
DD254211A5 (de) 1988-02-17
JPH06233686A (ja) 1994-08-23
EP0183071A3 (en) 1987-09-23
HUT38678A (en) 1986-06-30
ATE83263T1 (de) 1992-12-15
GR852611B (fr) 1986-03-04
PT81399B (pt) 1987-11-11
FI854142L (fi) 1986-05-01
EP0483115A3 (fr) 1992-05-06
KR930001117B1 (ko) 1993-02-18
JPH08196275A (ja) 1996-08-06
HU205627B (en) 1992-05-28
EP0183071B1 (fr) 1992-12-09
CN1011243B (zh) 1991-01-16
JP2552808B2 (ja) 1996-11-13
DE3586889D1 (de) 1993-01-21
YU169585A (en) 1991-04-30
DK496385A (da) 1986-05-01
IE930804L (en) 1986-04-30
CA1340733C (fr) 1999-09-14
JPS61173781A (ja) 1986-08-05
AU4875285A (en) 1986-06-12
ES548307A0 (es) 1986-09-01
EP0483115A2 (fr) 1992-04-29
PL158965B1 (pl) 1992-10-30
FI94427C (fi) 1995-09-11
JP2552807B2 (ja) 1996-11-13
AR242988A1 (es) 1993-06-30
NZ213842A (en) 1991-11-26
PT81399A (en) 1985-11-01
JP2502508B2 (ja) 1996-05-29
FI854142A0 (fi) 1985-10-23
ES8609458A1 (es) 1986-09-01
KR870003197A (ko) 1987-04-15
IL76765A (en) 1991-06-30
NO854312L (no) 1986-05-02
FI94427B (fi) 1995-05-31
DK496385D0 (da) 1985-10-29
DE3586889T2 (de) 1993-04-15

Similar Documents

Publication Publication Date Title
NO178076C (no) Fremgangsmåte til å fremstille heterologe proteiner i Pichia pastoris ved bruk av ekspresjonsvektorer inneholdende sekvenser som induseres av metanol
DK171284D0 (da) Fremgangsmaade til fremstilling af methanol i kemi-kvalitet
DK434381A (da) Fremgangsmaade til fremstilling af substituerede imonodicarboxylsyrer
DE3587377T2 (de) Verfahren zur herstellung von halbleiteranordnungen unter verwendung von silizium-auf- isolator techniken.
DE3680551D1 (de) Verfahren zur herstellung von halbleiteranordnungen mittels eines bondierungsverfahrens.
DK250184D0 (da) Fremgangsmaade til fremstilling af methanol
DK361281A (da) Fremgangsmaade til fremstilling af 2,5-diketo-d-gluconsyre
DK151968C (da) Fremgangsmaade til fremstilling af pentapeptidet h-arg-x-asp-y-tyr-r ved oploesningssyntese
DE3580626D1 (de) Verfahren zur herstellung von schleifmitteln.
DE58901324D1 (de) Verfahren zur herstellung von solarsilicium.
DE3587839T2 (de) Verfahren zur Herstellung von L-Phenylalanin.
NO844675L (no) Fremgangsmaate for fremstilling av metanol i vaeskefase.
DK112589A (da) Fremgangsmaade til fremstilling af l-alpha-aminosyrer
DK436685D0 (da) Fremgangsmaade til fremstilling af 2,4-dichlor-5-fluor-benzoesyre
DK24083D0 (da) Fremgangsmade til fremstilling af 0,0'-dithiodibenzoesyrer
DK147885D0 (da) Fremgangsmaade til fremstilling af glycosidbindingrelateret antigen
DE3679412D1 (de) Verfahren zur herstellung von halbleiteranordnungen mittels fluessiger epitaxie.
DK162997C (da) Fremgangsmaade til fremstilling af 5,6,7,8-tetrahydrofolinsyre
DE3678189D1 (de) Verfahren zur herstellung von aminoalkoholen durch elektrochemische reduktion von nitroalkoholen.
DK231987D0 (da) Fremgangsmaade til fremstilling af 2,3-epoxyamider
AT386973B (de) Verfahren zur herstellung von wendemessern
DK164923C (da) Fremgangsmaade til fremstilling af l-alfa-aminosyrer
DK295885D0 (da) Fremgangsmaade til fremstilling af 1-azidoaldoser
AT387716B (de) Verfahren zur herstellung von 2-brom-alphaergocriptin enthaltenden zusammensetzungen
DK413787D0 (da) Fremgangsmaade til fremstilling af 2,4-diamino-5-benzylpyrimidiner

Legal Events

Date Code Title Description
MK1K Patent expired