MY177814A - Rna-guided transcriptional regulation - Google Patents

Rna-guided transcriptional regulation

Info

Publication number
MY177814A
MY177814A MYPI2015002877A MYPI2015002877A MY177814A MY 177814 A MY177814 A MY 177814A MY PI2015002877 A MYPI2015002877 A MY PI2015002877A MY PI2015002877 A MYPI2015002877 A MY PI2015002877A MY 177814 A MY177814 A MY 177814A
Authority
MY
Malaysia
Prior art keywords
nucleic acid
cell
rnas
domain
dna
Prior art date
Application number
MYPI2015002877A
Other languages
English (en)
Inventor
George M Church
Prashant G Mali
Kevin M Esvelt
Original Assignee
Harvard College
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=52008736&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=MY177814(A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Harvard College filed Critical Harvard College
Publication of MY177814A publication Critical patent/MY177814A/en

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    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
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    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/35—Nature of the modification
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  • Physics & Mathematics (AREA)
  • Mycology (AREA)
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  • Cell Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Peptides Or Proteins (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
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MYPI2015002877A 2013-06-04 2014-06-04 Rna-guided transcriptional regulation MY177814A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201361830787P 2013-06-04 2013-06-04
PCT/US2014/040868 WO2014197568A2 (en) 2013-06-04 2014-06-04 Rna-guideded transcriptional regulation

Publications (1)

Publication Number Publication Date
MY177814A true MY177814A (en) 2020-09-23

Family

ID=52008736

Family Applications (3)

Application Number Title Priority Date Filing Date
MYPI2015002877A MY177814A (en) 2013-06-04 2014-06-04 Rna-guided transcriptional regulation
MYPI2019003198A MY197877A (en) 2013-06-04 2014-06-04 Rna-guided transcriptional regulation
MYPI2021007087A MY208492A (en) 2013-06-04 2014-06-04 Rna-guided transcriptional regulation

Family Applications After (2)

Application Number Title Priority Date Filing Date
MYPI2019003198A MY197877A (en) 2013-06-04 2014-06-04 Rna-guided transcriptional regulation
MYPI2021007087A MY208492A (en) 2013-06-04 2014-06-04 Rna-guided transcriptional regulation

Country Status (15)

Country Link
US (2) US11981917B2 (enExample)
EP (4) EP3003392B1 (enExample)
JP (5) JP6621738B2 (enExample)
KR (3) KR102884236B1 (enExample)
CN (2) CN113846096B (enExample)
AU (4) AU2014274939B2 (enExample)
CA (2) CA2914638A1 (enExample)
ES (1) ES2930537T3 (enExample)
IL (3) IL302205B2 (enExample)
MX (2) MX385330B (enExample)
MY (3) MY177814A (enExample)
RU (2) RU2756865C2 (enExample)
SG (3) SG10201710030QA (enExample)
WO (1) WO2014197568A2 (enExample)
ZA (1) ZA201509025B (enExample)

Families Citing this family (121)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6261500B2 (ja) 2011-07-22 2018-01-17 プレジデント アンド フェローズ オブ ハーバード カレッジ ヌクレアーゼ切断特異性の評価および改善
US10501791B2 (en) 2011-10-14 2019-12-10 President And Fellows Of Harvard College Sequencing by structure assembly
ES2991004T3 (es) 2011-12-22 2024-12-02 Harvard College Métodos para la detección de analitos
WO2014163886A1 (en) 2013-03-12 2014-10-09 President And Fellows Of Harvard College Method of generating a three-dimensional nucleic acid containing matrix
US9637739B2 (en) * 2012-03-20 2017-05-02 Vilnius University RNA-directed DNA cleavage by the Cas9-crRNA complex
WO2014065596A1 (en) * 2012-10-23 2014-05-01 Toolgen Incorporated Composition for cleaving a target dna comprising a guide rna specific for the target dna and cas protein-encoding nucleic acid or cas protein, and use thereof
WO2014093709A1 (en) 2012-12-12 2014-06-19 The Broad Institute, Inc. Methods, models, systems, and apparatus for identifying target sequences for cas enzymes or crispr-cas systems for target sequences and conveying results thereof
EP4299741A3 (en) 2012-12-12 2024-02-28 The Broad Institute, Inc. Delivery, engineering and optimization of systems, methods and compositions for sequence manipulation and therapeutic applications
WO2014093701A1 (en) 2012-12-12 2014-06-19 The Broad Institute, Inc. Functional genomics using crispr-cas systems, compositions, methods, knock out libraries and applications thereof
EP4481048A3 (en) * 2012-12-17 2025-02-26 President and Fellows of Harvard College Rna-guided human genome engineering
US9234213B2 (en) 2013-03-15 2016-01-12 System Biosciences, Llc Compositions and methods directed to CRISPR/Cas genomic engineering systems
US9828582B2 (en) 2013-03-19 2017-11-28 Duke University Compositions and methods for the induction and tuning of gene expression
MY177814A (en) 2013-06-04 2020-09-23 Harvard College Rna-guided transcriptional regulation
US9267135B2 (en) 2013-06-04 2016-02-23 President And Fellows Of Harvard College RNA-guided transcriptional regulation
KR102733409B1 (ko) * 2013-06-05 2024-11-21 듀크 유니버시티 Rna-가이드 유전자 편집 및 유전자 조절
KR20160056869A (ko) 2013-06-17 2016-05-20 더 브로드 인스티튜트, 인코퍼레이티드 바이러스 구성성분을 사용하여 장애 및 질환을 표적화하기 위한 crispr-cas 시스템 및 조성물의 전달, 용도 및 치료 적용
DK3011029T3 (da) 2013-06-17 2020-03-16 Broad Inst Inc Administration, modificering og optimering af tandem-guidesystemer, fremgangsmåder og sammensætninger til sekvensmanipulering
WO2014204727A1 (en) 2013-06-17 2014-12-24 The Broad Institute Inc. Functional genomics using crispr-cas systems, compositions methods, screens and applications thereof
EP3011031B1 (en) 2013-06-17 2020-09-30 The Broad Institute Inc. Delivery and use of the crispr-cas systems, vectors and compositions for hepatic targeting and therapy
CN105492611A (zh) * 2013-06-17 2016-04-13 布罗德研究所有限公司 用于序列操纵的优化的crispr-cas双切口酶系统、方法以及组合物
US20150044192A1 (en) 2013-08-09 2015-02-12 President And Fellows Of Harvard College Methods for identifying a target site of a cas9 nuclease
US9359599B2 (en) 2013-08-22 2016-06-07 President And Fellows Of Harvard College Engineered transcription activator-like effector (TALE) domains and uses thereof
US9228207B2 (en) 2013-09-06 2016-01-05 President And Fellows Of Harvard College Switchable gRNAs comprising aptamers
US9322037B2 (en) 2013-09-06 2016-04-26 President And Fellows Of Harvard College Cas9-FokI fusion proteins and uses thereof
US9737604B2 (en) 2013-09-06 2017-08-22 President And Fellows Of Harvard College Use of cationic lipids to deliver CAS9
WO2015040402A1 (en) * 2013-09-18 2015-03-26 Kymab Limited Methods. cells & organisms
PT3066201T (pt) 2013-11-07 2018-06-04 Massachusetts Inst Technology Métodos e composições relacionadas com crispr com arng reguladores
KR20250068794A (ko) 2013-12-12 2025-05-16 더 브로드 인스티튜트, 인코퍼레이티드 게놈 편집을 위한 ｃｒｉｓｐｒ-ｃａｓ 시스템 및 조성물의 전달, 용도 및 치료적 응용
CA2932479A1 (en) 2013-12-12 2015-06-18 The Rockefeller University Delivery, use and therapeutic applications of the crispr-cas systems and compositions for hbv and viral diseases and disorders
WO2015089364A1 (en) 2013-12-12 2015-06-18 The Broad Institute Inc. Crystal structure of a crispr-cas system, and uses thereof
CN106103705A (zh) 2013-12-12 2016-11-09 布罗德研究所有限公司 核苷酸重复障碍中crispr‑cas系统的组合物和使用方法
US9068179B1 (en) 2013-12-12 2015-06-30 President And Fellows Of Harvard College Methods for correcting presenilin point mutations
WO2015089473A1 (en) 2013-12-12 2015-06-18 The Broad Institute Inc. Engineering of systems, methods and optimized guide compositions with new architectures for sequence manipulation
WO2015089486A2 (en) 2013-12-12 2015-06-18 The Broad Institute Inc. Systems, methods and compositions for sequence manipulation with optimized functional crispr-cas systems
EP3114227B1 (en) 2014-03-05 2021-07-21 Editas Medicine, Inc. Crispr/cas-related methods and compositions for treating usher syndrome and retinitis pigmentosa
US11141493B2 (en) 2014-03-10 2021-10-12 Editas Medicine, Inc. Compositions and methods for treating CEP290-associated disease
US11339437B2 (en) 2014-03-10 2022-05-24 Editas Medicine, Inc. Compositions and methods for treating CEP290-associated disease
EP3116997B1 (en) 2014-03-10 2019-05-15 Editas Medicine, Inc. Crispr/cas-related methods and compositions for treating leber's congenital amaurosis 10 (lca10)
WO2015139139A1 (en) * 2014-03-20 2015-09-24 UNIVERSITé LAVAL Crispr-based methods and products for increasing frataxin levels and uses thereof
US11242525B2 (en) 2014-03-26 2022-02-08 Editas Medicine, Inc. CRISPR/CAS-related methods and compositions for treating sickle cell disease
EP3126495A1 (en) 2014-04-02 2017-02-08 Editas Medicine, Inc. Crispr/cas-related methods and compositions for treating primary open angle glaucoma
US10179932B2 (en) 2014-07-11 2019-01-15 President And Fellows Of Harvard College Methods for high-throughput labelling and detection of biological features in situ using microscopy
US10077453B2 (en) 2014-07-30 2018-09-18 President And Fellows Of Harvard College CAS9 proteins including ligand-dependent inteins
NZ728437A (en) 2014-08-27 2018-02-23 Caribou Biosciences Inc Methods for increasing cas9-mediated engineering efficiency
AU2015330699B2 (en) 2014-10-10 2021-12-02 Editas Medicine, Inc. Compositions and methods for promoting homology directed repair
AU2015342749B2 (en) 2014-11-07 2022-01-27 Editas Medicine, Inc. Methods for improving CRISPR/Cas-mediated genome-editing
EP4400584A3 (en) 2014-12-03 2024-10-16 Agilent Technologies, Inc. Guide rna with chemical modifications
WO2016094874A1 (en) * 2014-12-12 2016-06-16 The Broad Institute Inc. Escorted and functionalized guides for crispr-cas systems
WO2016094867A1 (en) 2014-12-12 2016-06-16 The Broad Institute Inc. Protected guide rnas (pgrnas)
WO2016106244A1 (en) 2014-12-24 2016-06-30 The Broad Institute Inc. Crispr having or associated with destabilization domains
JP6929791B2 (ja) 2015-02-09 2021-09-01 デューク ユニバーシティ エピゲノム編集のための組成物および方法
EP3280803B1 (en) 2015-04-06 2021-05-26 The Board of Trustees of the Leland Stanford Junior University Chemically modified guide rnas for crispr/cas-mediated gene regulation
GB201506509D0 (en) 2015-04-16 2015-06-03 Univ Wageningen Nuclease-mediated genome editing
WO2016172727A1 (en) 2015-04-24 2016-10-27 Editas Medicine, Inc. Evaluation of cas9 molecule/guide rna molecule complexes
CA2986310A1 (en) 2015-05-11 2016-11-17 Editas Medicine, Inc. Optimized crispr/cas9 systems and methods for gene editing in stem cells
WO2016196539A2 (en) 2015-06-01 2016-12-08 Temple University - Of The Commonwealth System Of Higher Education Methods and compositions for rna-guided treatment of hiv infection
EP3307887A1 (en) 2015-06-09 2018-04-18 Editas Medicine, Inc. Crispr/cas-related methods and compositions for improving transplantation
US9790490B2 (en) * 2015-06-18 2017-10-17 The Broad Institute Inc. CRISPR enzymes and systems
KR102840885B1 (ko) 2015-06-18 2025-07-30 더 브로드 인스티튜트, 인코퍼레이티드 표적외 효과를 감소시키는 crispr 효소 돌연변이
US10648020B2 (en) 2015-06-18 2020-05-12 The Broad Institute, Inc. CRISPR enzymes and systems
WO2016205759A1 (en) 2015-06-18 2016-12-22 The Broad Institute Inc. Engineering and optimization of systems, methods, enzymes and guide scaffolds of cas9 orthologs and variants for sequence manipulation
WO2017053879A1 (en) 2015-09-24 2017-03-30 Editas Medicine, Inc. Use of exonucleases to improve crispr/cas-mediated genome editing
EP3365356B1 (en) 2015-10-23 2023-06-28 President and Fellows of Harvard College Nucleobase editors and uses thereof
GB2559526B (en) 2015-11-03 2021-02-17 Harvard College Method and apparatus for volumetric imaging of a three-dimensional nucleic acid containing matrix
WO2017095967A2 (en) 2015-11-30 2017-06-08 Duke University Therapeutic targets for the correction of the human dystrophin gene by gene editing and methods of use
US12110490B2 (en) 2015-12-18 2024-10-08 The Broad Institute, Inc. CRISPR enzymes and systems
JP6914274B2 (ja) * 2016-01-22 2021-08-04 ザ・ブロード・インスティテュート・インコーポレイテッド Ｃｒｉｓｐｒｃｐｆ１の結晶構造
EP3433364A1 (en) 2016-03-25 2019-01-30 Editas Medicine, Inc. Systems and methods for treating alpha 1-antitrypsin (a1at) deficiency
EP3433363A1 (en) 2016-03-25 2019-01-30 Editas Medicine, Inc. Genome editing systems comprising repair-modulating enzyme molecules and methods of their use
US20190127713A1 (en) 2016-04-13 2019-05-02 Duke University Crispr/cas9-based repressors for silencing gene targets in vivo and methods of use
US11236313B2 (en) 2016-04-13 2022-02-01 Editas Medicine, Inc. Cas9 fusion molecules, gene editing systems, and methods of use thereof
US12410416B2 (en) 2016-04-19 2025-09-09 The Broad Institute, Inc. CRISPR enzymes and systems with modified pam specificity
EP3445853A1 (en) 2016-04-19 2019-02-27 The Broad Institute, Inc. Cpf1 complexes with reduced indel activity
EP4613756A3 (en) 2016-04-25 2025-11-12 President And Fellows Of Harvard College Hybridization chain reaction methods for in situ molecular detection
DK3272867T3 (da) * 2016-06-02 2019-12-02 Sigma Aldrich Co Llc Anvendelse af programmerbare dna-bindingsproteiner til forbedring af målrettet genommodifikation
US10767175B2 (en) 2016-06-08 2020-09-08 Agilent Technologies, Inc. High specificity genome editing using chemically modified guide RNAs
EP3472321A2 (en) * 2016-06-17 2019-04-24 Genesis Technologies Limited Crispr-cas system, materials and methods
EP4275747A3 (en) 2016-07-19 2024-01-24 Duke University Therapeutic applications of cpf1-based genome editing
EP3494220A1 (en) 2016-08-02 2019-06-12 Editas Medicine, Inc. Compositions and methods for treating cep290 associated disease
JP7231935B2 (ja) 2016-08-03 2023-03-08 プレジデント アンド フェローズ オブ ハーバード カレッジ アデノシン核酸塩基編集因子およびそれらの使用
EP3497214B1 (en) 2016-08-09 2023-06-28 President and Fellows of Harvard College Programmable cas9-recombinase fusion proteins and uses thereof
WO2018039438A1 (en) 2016-08-24 2018-03-01 President And Fellows Of Harvard College Incorporation of unnatural amino acids into proteins using base editing
EP3526320A1 (en) 2016-10-14 2019-08-21 President and Fellows of Harvard College Aav delivery of nucleobase editors
WO2018119010A1 (en) 2016-12-19 2018-06-28 Editas Medicine, Inc. Assessing nuclease cleavage
US10745677B2 (en) 2016-12-23 2020-08-18 President And Fellows Of Harvard College Editing of CCR5 receptor gene to protect against HIV infection
JP7229923B2 (ja) 2017-01-06 2023-02-28 エディタス・メディシン、インコーポレイテッド ヌクレアーゼ切断を評価する方法
TW201839136A (zh) 2017-02-06 2018-11-01 瑞士商諾華公司 治療血色素異常症之組合物及方法
WO2018165504A1 (en) 2017-03-09 2018-09-13 President And Fellows Of Harvard College Suppression of pain by gene editing
WO2018165631A1 (en) 2017-03-09 2018-09-13 President And Fellows Of Harvard College Cancer vaccine
KR20190127797A (ko) 2017-03-10 2019-11-13 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 시토신에서 구아닌으로의 염기 편집제
EP3596217A1 (en) 2017-03-14 2020-01-22 Editas Medicine, Inc. Systems and methods for the treatment of hemoglobinopathies
KR20240116572A (ko) 2017-03-23 2024-07-29 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 핵산 프로그램가능한 dna 결합 단백질을 포함하는 핵염기 편집제
WO2018179578A1 (ja) * 2017-03-30 2018-10-04 国立大学法人京都大学 ゲノム編集によるエクソンスキッピング誘導方法
CN116555353A (zh) 2017-04-20 2023-08-08 E开创生物技术股份有限公司 产生基因修改的动物的方法
WO2018201086A1 (en) 2017-04-28 2018-11-01 Editas Medicine, Inc. Methods and systems for analyzing guide rna molecules
WO2018209158A2 (en) 2017-05-10 2018-11-15 Editas Medicine, Inc. Crispr/rna-guided nuclease systems and methods
US11560566B2 (en) 2017-05-12 2023-01-24 President And Fellows Of Harvard College Aptazyme-embedded guide RNAs for use with CRISPR-Cas9 in genome editing and transcriptional activation
MX2019014640A (es) 2017-06-09 2020-10-05 Editas Medicine Inc Nucleasas de repeticiones palíndromicas agrupadas y regularmente interespaciadas de proteina asociada 9 (cas9) genomanipuladas.
CN107266583B (zh) * 2017-06-27 2021-02-09 深圳大学 一种基于格氏嗜盐碱杆菌Argonaute靶向激活基因转录的方法
US11866726B2 (en) 2017-07-14 2024-01-09 Editas Medicine, Inc. Systems and methods for targeted integration and genome editing and detection thereof using integrated priming sites
WO2019023680A1 (en) 2017-07-28 2019-01-31 President And Fellows Of Harvard College METHODS AND COMPOSITIONS FOR EVOLUTION OF BASIC EDITORS USING PHAGE-ASSISTED CONTINUOUS EVOLUTION (PACE)
EP3676376B1 (en) 2017-08-30 2025-01-15 President and Fellows of Harvard College High efficiency base editors comprising gam
CN111757937A (zh) 2017-10-16 2020-10-09 布罗德研究所股份有限公司 腺苷碱基编辑器的用途
WO2019118949A1 (en) 2017-12-15 2019-06-20 The Broad Institute, Inc. Systems and methods for predicting repair outcomes in genetic engineering
JP7706888B2 (ja) 2018-03-14 2025-07-14 エディタス・メディシン、インコーポレイテッド 異常ヘモグロビン症の治療のためのシステム及び方法
EP3592140A1 (en) 2018-03-19 2020-01-15 Regeneron Pharmaceuticals, Inc. Transcription modulation in animals using crispr/cas systems
US12157760B2 (en) 2018-05-23 2024-12-03 The Broad Institute, Inc. Base editors and uses thereof
EP3575402A1 (en) * 2018-06-01 2019-12-04 Algentech SAS Gene targeting
CA3104856A1 (en) 2018-06-29 2020-01-02 Editas Medicine, Inc. Synthetic guide molecules, compositions and methods relating thereto
GB201813011D0 (en) 2018-08-10 2018-09-26 Vib Vzw Means and methods for drought tolerance in crops
WO2020076976A1 (en) 2018-10-10 2020-04-16 Readcoor, Inc. Three-dimensional spatial molecular indexing
US12281338B2 (en) 2018-10-29 2025-04-22 The Broad Institute, Inc. Nucleobase editors comprising GeoCas9 and uses thereof
WO2020154500A1 (en) 2019-01-23 2020-07-30 The Broad Institute, Inc. Supernegatively charged proteins and uses thereof
MX2021011426A (es) 2019-03-19 2022-03-11 Broad Inst Inc Metodos y composiciones para editar secuencias de nucleótidos.
EP3956349A1 (en) 2019-04-17 2022-02-23 The Broad Institute, Inc. Adenine base editors with reduced off-target effects
US12435330B2 (en) 2019-10-10 2025-10-07 The Broad Institute, Inc. Methods and compositions for prime editing RNA
JP7525140B2 (ja) * 2020-01-31 2024-07-30 国立大学法人京都大学 タンパク質翻訳の制御システム
MX2022014008A (es) 2020-05-08 2023-02-09 Broad Inst Inc Métodos y composiciones para la edición simultánea de ambas cadenas de una secuencia de nucleótidos de doble cadena objetivo.
US20220049303A1 (en) 2020-08-17 2022-02-17 Readcoor, Llc Methods and systems for spatial mapping of genetic variants
KR20240055811A (ko) 2021-09-10 2024-04-29 애질런트 테크놀로지스, 인크. 프라임 편집을 위한 화학적 변형을 갖는 가이드 rna
WO2024193739A1 (en) * 2023-03-23 2024-09-26 Ostravska Univerzita Method for producing proteins in host cells

Family Cites Families (161)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4123610A (en) 1977-03-09 1978-10-31 The United States Government Nucleic acid crosslinking agent and affinity inactivation of nucleic acids therewith
US5525464A (en) 1987-04-01 1996-06-11 Hyseq, Inc. Method of sequencing by hybridization of oligonucleotide probes
US4981985A (en) 1987-10-20 1991-01-01 Trustees Of The University Of Pennsylvania Synthesis of photolabile chelators for multivalent cations
US4844617A (en) 1988-01-20 1989-07-04 Tencor Instruments Confocal measuring microscope with automatic focusing
US5151189A (en) 1990-09-17 1992-09-29 Gelman Sciences, Inc. Cationic charge modified microporous membrane
WO1993016176A1 (en) 1992-02-13 1993-08-19 Bio-Metric Systems, Inc. Immobilization of chemical species in crosslinked matrices
US5594235A (en) 1993-06-17 1997-01-14 Ultrapointe Corporation Automated surface acquisition for a confocal microscope
US5578832A (en) 1994-09-02 1996-11-26 Affymetrix, Inc. Method and apparatus for imaging a sample on a device
SE9400522D0 (sv) 1994-02-16 1994-02-16 Ulf Landegren Method and reagent for detecting specific nucleotide sequences
US5750341A (en) 1995-04-17 1998-05-12 Lynx Therapeutics, Inc. DNA sequencing by parallel oligonucleotide extensions
US5830708A (en) 1995-06-06 1998-11-03 Advanced Tissue Sciences, Inc. Methods for production of a naturally secreted extracellular matrix
US6284284B1 (en) 1995-06-06 2001-09-04 Advanced Tissue Sciences, Inc. Compositions and methods for production and use of an injectable naturally secreted extracellular matrix
JP4124377B2 (ja) 1996-06-06 2008-07-23 ソレクサ・インコーポレイテッド コードアダプターの連結による配列決定
US5948617A (en) 1997-06-13 1999-09-07 Biospeparations, Inc. Methods of in situ hybridization
US6083726A (en) 1998-02-03 2000-07-04 Lucent Technologies, Inc. Methods for polynucleotide synthesis and articles for polynucleotide hybridization
AU754952B2 (en) 1998-06-24 2002-11-28 Illumina, Inc. Decoding of array sensors with microspheres
DE60044490D1 (de) 1999-02-23 2010-07-15 Caliper Life Sciences Inc Manipulation von mikroteilchen in mikrofluiden systemen
EP1171636A2 (en) 1999-04-22 2002-01-16 Albert Einstein College Of Medicine Of Yeshiva University Assay of gene expression patterns by multi-fluor fish
US20020015952A1 (en) 1999-07-30 2002-02-07 Anderson Norman G. Microarrays and their manufacture by slicing
EP1242131A4 (en) 1999-10-12 2006-05-03 Univ Ohio State REACTIVE POLYMER VALVE, DISTRIBUTION DEVICES AND METHODS OF USING THE SAME
AU1774501A (en) 1999-11-17 2001-05-30 Research Foundation Of State University Of New York, The Three dimensional data storage device and method for reading
AU2001247414A1 (en) 2000-03-14 2001-09-24 Active Motif Oligonucleotide analogues, methods of synthesis and methods of use
JP2003535600A (ja) 2000-06-02 2003-12-02 バイエル コーポレイション インサイチュ分枝ｄｎａハイブリダイゼーションを用いた高感度遺伝子検出および位置決定
ATE447043T1 (de) 2000-12-14 2009-11-15 Gen Probe Inc Verfahren und kits zur verbesserung der hybridisierungsraten von polynukleotiden
DK1370690T3 (da) 2001-03-16 2012-07-09 Kalim Mir Arrays og fremgangsmåder til anvendelse heraf
US7473767B2 (en) 2001-07-03 2009-01-06 The Institute For Systems Biology Methods for detection and quantification of analytes in complex mixtures
US20060248349A1 (en) 2001-09-24 2006-11-02 Rathjen Alicemarie G Method for preparing and using personal and genetic profiles
WO2003031591A2 (en) 2001-10-10 2003-04-17 Superarray Bioscience Corporation Detecting targets by unique identifier nucleotide tags
GB2382137A (en) 2001-11-20 2003-05-21 Mats Gullberg Nucleic acid enrichment
US20090246879A1 (en) 2002-12-20 2009-10-01 Callida Genomics Materials and Methods Relating to Nano-Tags and Nano-Brands
EP1594980B2 (en) 2003-01-29 2017-08-16 454 Life Sciences Corporation Bead emulsion nucleic acid amplification
EP2365095A1 (en) 2003-02-26 2011-09-14 Callida Genomics, Inc. Random array DNA analysis by hybridization
US7394592B2 (en) 2003-05-20 2008-07-01 Lucid, Inc. Confocal microscope for imaging of selected locations of the body of a patient
WO2005047543A2 (en) 2003-06-10 2005-05-26 Applera Corporation Ligation assay
US7193054B2 (en) 2003-08-26 2007-03-20 University Of Rochester Nanofabrication using actin filaments
US20080050718A1 (en) 2003-11-14 2008-02-28 Gesteland Raymond F Methods, Articles, and Compositions for Identifying Oligonucleotides
US7381529B2 (en) 2003-12-31 2008-06-03 Intel Corporation Methods and compositions for detecting nucleic acids using scanning probe microscopy and nanocodes
EP1718769A4 (en) 2004-02-27 2008-05-14 Harvard College Polonie FLUORESCENCE IN SITU SEQUENZIERKÜGELCHEN
WO2005085850A1 (en) 2004-02-27 2005-09-15 Tianxin Wang Methods for multiplexed analyte detection
WO2006076017A2 (en) 2004-04-30 2006-07-20 Applera Corporation Methods and kits for identifying target nucleotides in mixed populations
US20060024711A1 (en) 2004-07-02 2006-02-02 Helicos Biosciences Corporation Methods for nucleic acid amplification and sequence determination
US7253947B2 (en) 2004-10-07 2007-08-07 New York University Portable automated confocal microscope
NZ555644A (en) * 2004-11-09 2009-04-30 Santaris Pharma As Potent LNA oligonucleotides for the inhibition of HIF-1A expression
US7393665B2 (en) 2005-02-10 2008-07-01 Population Genetics Technologies Ltd Methods and compositions for tagging and identifying polynucleotides
ITRM20050068A1 (it) 2005-02-17 2006-08-18 Istituto Naz Per Le Malattie I Metodo per la rivelazione di acidi nucleici di agenti patogeni batterici o di parassiti nelle urine.
US20060234261A1 (en) 2005-03-08 2006-10-19 Pierce Niles A Colorimetric readout of hybridization chain reaction
US7727721B2 (en) 2005-03-08 2010-06-01 California Institute Of Technology Hybridization chain reaction amplification for in situ imaging
US20070020650A1 (en) 2005-04-01 2007-01-25 Avak Kahvejian Methods for detecting proteins
MX2007012486A (es) 2005-04-08 2007-12-13 Mycosol Inc Ensayos de investigacion sobre estilbazio.
EP1910537A1 (en) 2005-06-06 2008-04-16 454 Life Sciences Corporation Paired end sequencing
US7842793B2 (en) 2005-06-14 2010-11-30 The California Institute Of Technology Methods of making nucleic acid nanostructures
EP1907571B1 (en) 2005-06-15 2017-04-26 Complete Genomics Inc. Nucleic acid analysis by random mixtures of non-overlapping fragments
CN101495650B (zh) 2005-06-20 2015-02-04 领先细胞医疗诊断有限公司 检测单个细胞中的核酸和鉴定异质大细胞群中罕见细胞的方法
US8486621B2 (en) 2005-08-11 2013-07-16 Cornell Research Foundation, Inc. Nucleic acid-based matrixes
CA2640385C (en) 2005-12-23 2014-07-15 Nanostring Technologies, Inc. Nanoreporters and methods of manufacturing and use thereof
US7864996B2 (en) 2006-02-17 2011-01-04 Lucid, Inc. System for macroscopic and confocal imaging of tissue
US20090220968A1 (en) 2006-03-10 2009-09-03 President And Fellows Of Harvard College Methods and Apparatus for Near Field Irradiation
GB0605584D0 (en) 2006-03-20 2006-04-26 Olink Ab Method for analyte detection using proximity probes
US20070231823A1 (en) 2006-03-23 2007-10-04 Mckernan Kevin J Directed enrichment of genomic DNA for high-throughput sequencing
US8975216B2 (en) 2006-03-30 2015-03-10 Pacific Biosciences Of California Articles having localized molecules disposed thereon and methods of producing same
EP2018622B1 (en) 2006-03-31 2018-04-25 Illumina, Inc. Systems for sequence by synthesis analysis
CN101495654A (zh) 2006-04-19 2009-07-29 阿普里拉股份有限公司 无凝胶珠基测序的试剂、方法和文库
US7964347B2 (en) 2006-06-15 2011-06-21 Krassen Dimitrov Labels for electronic detection of individual molecules and methods for their detection
WO2007149696A1 (en) 2006-06-23 2007-12-27 Applera Corporation Systems and methods for cooling in biological analysis instruments
US7745129B1 (en) 2006-07-12 2010-06-29 Kenneth David Schatz Methods for sequencing of a necleic acid
WO2008033848A2 (en) 2006-09-11 2008-03-20 The Arizona Board Of Regents, A Body Corporate Of The State Of Arizona Acting For And On Behalf Of Arizona State University Self-assembled combinatorial encoding nanoarrays for multiplexed biosensing
US20090208965A1 (en) 2006-10-25 2009-08-20 Ikonisys, Inc. Automated method for detecting cancers and high grade hyperplasias
US9201063B2 (en) 2006-11-16 2015-12-01 General Electric Company Sequential analysis of biological samples
GB2457402B (en) 2006-12-01 2011-10-19 Univ Columbia Four-color DNA sequencing by synthesis using cleavable fluorescent nucleotide reversible terminators
US20080269068A1 (en) 2007-02-06 2008-10-30 President And Fellows Of Harvard College Multiplex decoding of sequence tags in barcodes
NZ579002A (en) 2007-03-02 2012-03-30 Danisco Cultures with improved phage resistance
US8145677B2 (en) 2007-03-27 2012-03-27 Faleh Jassem Al-Shameri Automated generation of metadata for mining image and text data
US9217151B2 (en) 2007-05-16 2015-12-22 California Institute Of Technology Versatile nucleic acid hairpin motif for programming biomolecular self-assembly pathways
JP5745842B2 (ja) 2007-06-19 2015-07-08 ストラトス ゲノミクス インコーポレイテッド 拡張によるハイスループット核酸配列決定
WO2009018576A1 (en) 2007-08-02 2009-02-05 Biodesic Compositions and methods for analyte detection and quantitation
WO2009046149A1 (en) 2007-10-01 2009-04-09 Applied Biosystems Inc. Chase ligation sequencing
CN101889074A (zh) 2007-10-04 2010-11-17 哈尔西恩莫尔丘勒公司 采用电子显微镜对核酸聚合物测序
US20090139311A1 (en) 2007-10-05 2009-06-04 Applied Biosystems Inc. Biological Analysis Systems, Devices, and Methods
US20110020291A1 (en) 2007-11-17 2011-01-27 Debrabrata Banerjee Use of stem cells for wound healing
CN101586150B (zh) 2008-05-23 2016-09-28 陕西佰美基因股份有限公司 检测探针、通用寡核苷酸芯片及核酸检测方法及其用途
US8546553B2 (en) 2008-07-25 2013-10-01 University Of Georgia Research Foundation, Inc. Prokaryotic RNAi-like system and methods of use
CN102171234A (zh) 2008-08-05 2011-08-31 康奈尔大学 光交联核酸水凝胶
WO2010019826A1 (en) 2008-08-14 2010-02-18 Nanostring Technologies, Inc Stable nanoreporters
US20100076057A1 (en) * 2008-09-23 2010-03-25 Northwestern University TARGET DNA INTERFERENCE WITH crRNA
US9156010B2 (en) 2008-09-23 2015-10-13 Bio-Rad Laboratories, Inc. Droplet-based assay system
US20100087325A1 (en) 2008-10-07 2010-04-08 Illumina, Inc. Biological sample temperature control system and method
US9404098B2 (en) 2008-11-06 2016-08-02 University Of Georgia Research Foundation, Inc. Method for cleaving a target RNA using a Cas6 polypeptide
US8309306B2 (en) 2008-11-12 2012-11-13 Nodality, Inc. Detection composition
EP3150724A1 (en) 2008-12-19 2017-04-05 President and Fellows of Harvard College Particle-assisted nucleic acid sequencing
JP4528345B2 (ja) 2009-01-28 2010-08-18 シャープ株式会社 動画再生装置、動画再生方法、動画再生方法をコンピュータで実現するためのプログラム及びそのプログラムを記録した記録媒体
FR2948475A1 (fr) 2009-07-24 2011-01-28 Bionext Procede de caracterisation d'objets tridimensionnels
US8431151B2 (en) 2009-08-06 2013-04-30 Syracuse University Antimicrobial nanostructured hydrogel web containing silver
EP3236264A3 (en) 2009-10-13 2017-11-08 Nanostring Technologies, Inc Protein detection via nanoreporters
CN102712955A (zh) 2009-11-03 2012-10-03 Htg分子诊断有限公司 定量核酸酶保护测序(qNPS)
WO2011075516A2 (en) 2009-12-18 2011-06-23 President And Fellows Of Harvard College Active scaffolds for on-demand drug and cell delivery
WO2011100617A2 (en) 2010-02-12 2011-08-18 Life Technologies Corporation Nucleic acid, biomolecule and polymer identifier codes
US10267808B2 (en) 2010-03-08 2019-04-23 California Institute Of Technology Molecular indicia of cellular constituents and resolving the same by super-resolution technologies in single cells
US10087431B2 (en) 2010-03-10 2018-10-02 The Regents Of The University Of California Methods of generating nucleic acid fragments
GB201004292D0 (en) 2010-03-15 2010-04-28 Olink Ab Assay for localised detection of analytes
RS54482B1 (sr) 2010-04-05 2016-06-30 Prognosys Biosciences, Inc. Biološka testiranja sa prostornim kodiranjem
EP2569425B1 (en) 2010-05-10 2016-07-06 The Regents of The University of California Endoribonuclease compositions and methods of use thereof
US20130059741A1 (en) 2010-05-13 2013-03-07 Illumina, Inc. Binding assays for markers
SMT201800213T1 (it) 2010-07-09 2018-07-17 Cergentis B V Strategie 3-d di sequenziamento di regioni genomiche di interesse
WO2012056440A1 (en) 2010-10-28 2012-05-03 Nanodoc Ltd. COMPOSITIONS AND METHODS FOR ACTIVATING EXPRESSION BY A SPECIFIC ENDOGENOUS miRNA
EP2633080B1 (en) 2010-10-29 2018-12-05 President and Fellows of Harvard College Method of detecting targets using fluorescently labelled nucleic acid nanotube probes
JP5998148B2 (ja) 2010-11-16 2016-09-28 ナブシス ２．０ エルエルシー ハイブリダイズされたプローブの相対位置を検出することによる生体分子のシークエンシングのための方法
WO2012110899A2 (en) 2011-02-15 2012-08-23 Bernitz Mats Nilsson Method for localizedin situdetection of mrna
JP5687514B2 (ja) 2011-02-17 2015-03-18 株式会社日立ハイテクノロジーズ 核酸配列解析装置
DK2500436T3 (en) 2011-03-17 2016-08-29 Pasteur Institut A method, probe and kit for in situ hybridization of DNA and use thereof
WO2012150035A1 (de) 2011-05-04 2012-11-08 Genovoxx Gmbh Nukleosid-triphosphat-konjugate und methoden zu deren anwendung
EP2714938B1 (en) 2011-05-27 2017-11-15 President and Fellows of Harvard College Methods of amplifying whole genome of a single cell
US20140113376A1 (en) 2011-06-01 2014-04-24 Rotem Sorek Compositions and methods for downregulating prokaryotic genes
PL222511B1 (pl) 2011-06-08 2016-08-31 Międzynarodowy Inst Biologii Molekularnej I Komórkowej W Warszawie Endorybonukleazy dsRNA
US10501791B2 (en) 2011-10-14 2019-12-10 President And Fellows Of Harvard College Sequencing by structure assembly
WO2014163886A1 (en) 2013-03-12 2014-10-09 President And Fellows Of Harvard College Method of generating a three-dimensional nucleic acid containing matrix
GB201122458D0 (en) 2011-12-30 2012-02-08 Univ Wageningen Modified cascade ribonucleoproteins and uses thereof
SG11201405103SA (en) 2012-02-24 2014-09-26 Hutchinson Fred Cancer Res Compositions and methods for the treatment of hemoglobinopathies
GB2513024B (en) 2012-02-27 2016-08-31 Cellular Res Inc A clonal amplification method
HK1203566A1 (zh) 2012-02-29 2015-10-30 桑格摩生物科学股份有限公司 治療亨廷頓氏病的方法和組合物
WO2013141680A1 (en) 2012-03-20 2013-09-26 Vilnius University RNA-DIRECTED DNA CLEAVAGE BY THE Cas9-crRNA COMPLEX
US9637739B2 (en) 2012-03-20 2017-05-02 Vilnius University RNA-directed DNA cleavage by the Cas9-crRNA complex
MY189533A (en) 2012-05-25 2022-02-16 Univ California Methods and compositions for rna-directed target dna modification and for rna-directed modulation of transcription
US9082418B2 (en) 2012-07-16 2015-07-14 Marvell International Ltd. Methods for reading data from a storage medium using a reader and storage devices
KR102134361B1 (ko) 2012-07-20 2020-08-26 가부시키가이샤 엘에스아이 메디엔스 광응답성 핵산류를 포함한 프로브를 이용한 광연결 방법
HK1207111A1 (en) 2012-08-03 2016-01-22 加利福尼亚大学董事会 Methods and compositions for controlling gene expression by rna processing
BR112015003464B1 (pt) 2012-08-15 2022-01-18 Lucid, Inc Sistemas e processos para formação de imagens de tecido
CN102908119A (zh) 2012-09-26 2013-02-06 温州医学院眼视光研究院 一种共焦扫描成像系统及其像差控制方法
WO2014065596A1 (en) 2012-10-23 2014-05-01 Toolgen Incorporated Composition for cleaving a target dna comprising a guide rna specific for the target dna and cas protein-encoding nucleic acid or cas protein, and use thereof
AU2013355214B2 (en) 2012-12-06 2017-06-15 Sigma-Aldrich Co. Llc Crispr-based genome modification and regulation
CN119752887A (zh) 2012-12-12 2025-04-04 布罗德研究所有限公司 用于序列操纵的系统、方法和优化的指导组合物的工程化
WO2014093694A1 (en) 2012-12-12 2014-06-19 The Broad Institute, Inc. Crispr-cas nickase systems, methods and compositions for sequence manipulation in eukaryotes
EP2931898B1 (en) 2012-12-12 2016-03-09 The Broad Institute, Inc. Engineering and optimization of systems, methods and compositions for sequence manipulation with functional domains
CA2894668A1 (en) 2012-12-12 2014-06-19 The Broad Institute, Inc. Crispr-cas systems and methods for altering expression of gene products in eukaryotic cells
US8697359B1 (en) 2012-12-12 2014-04-15 The Broad Institute, Inc. CRISPR-Cas systems and methods for altering expression of gene products
EP4299741A3 (en) 2012-12-12 2024-02-28 The Broad Institute, Inc. Delivery, engineering and optimization of systems, methods and compositions for sequence manipulation and therapeutic applications
EP4481048A3 (en) * 2012-12-17 2025-02-26 President and Fellows of Harvard College Rna-guided human genome engineering
CN104995302B (zh) 2013-01-16 2021-08-31 爱默蕾大学 Cas9-核酸复合物及其相关用途
US9411930B2 (en) 2013-02-01 2016-08-09 The Regents Of The University Of California Methods for genome assembly and haplotype phasing
WO2014150624A1 (en) 2013-03-14 2014-09-25 Caribou Biosciences, Inc. Compositions and methods of nucleic acid-targeting nucleic acids
US9330295B2 (en) 2013-03-15 2016-05-03 Brown University Spatial sequencing/gene expression camera
US9234213B2 (en) 2013-03-15 2016-01-12 System Biosciences, Llc Compositions and methods directed to CRISPR/Cas genomic engineering systems
US20140273230A1 (en) 2013-03-15 2014-09-18 Sigma-Aldrich Co., Llc Crispr-based genome modification and regulation
KR102210319B1 (ko) 2013-03-15 2021-02-01 더 제너럴 하스피탈 코포레이션 특정 게놈 좌위에 대한 유전적 및 후성적 조절 단백질의 rna-안내 표적화
FI2992115T4 (fi) 2013-04-30 2025-05-15 California Institute Of Technology Molekyylien limitetty leimaus sekventiaalisella hybridisaatioviivakoodauksella
US10510435B2 (en) 2013-04-30 2019-12-17 California Institute Of Technology Error correction of multiplex imaging analysis by sequential hybridization
US20160122774A1 (en) * 2013-05-29 2016-05-05 Cellectis A method for producing precise dna cleavage using cas9 nickase activity
US9267135B2 (en) * 2013-06-04 2016-02-23 President And Fellows Of Harvard College RNA-guided transcriptional regulation
MY177814A (en) * 2013-06-04 2020-09-23 Harvard College Rna-guided transcriptional regulation
CN105492611A (zh) 2013-06-17 2016-04-13 布罗德研究所有限公司 用于序列操纵的优化的crispr-cas双切口酶系统、方法以及组合物
EP3058091B1 (en) 2013-10-18 2020-03-25 The Broad Institute, Inc. Spatial and cellular mapping of biomolecules in situ by high-throughput sequencing
WO2015089473A1 (en) 2013-12-12 2015-06-18 The Broad Institute Inc. Engineering of systems, methods and optimized guide compositions with new architectures for sequence manipulation
GB201401885D0 (en) 2014-02-04 2014-03-19 Olink Ab Proximity assay with detection based on hybridisation chain reaction (HCR)
JP6456969B2 (ja) 2014-02-21 2019-01-23 マサチューセッツ インスティテュート オブ テクノロジー 膨張顕微鏡法
CN113403373B (zh) 2014-11-21 2025-04-11 布鲁克空间生物学公司 无酶且无扩增的测序
ES2836802T3 (es) 2015-02-27 2021-06-28 Becton Dickinson Co Códigos de barras moleculares espacialmente direccionables
EP4180535A1 (en) 2015-03-30 2023-05-17 Becton, Dickinson and Company Methods and compositions for combinatorial barcoding
GB2559526B (en) 2015-11-03 2021-02-17 Harvard College Method and apparatus for volumetric imaging of a three-dimensional nucleic acid containing matrix
US10386300B2 (en) 2015-12-21 2019-08-20 Verily Life Sciences Llc Spectrally and spatially multiplexed fluorescent probes for in situ cell labeling
US10844426B2 (en) 2016-03-17 2020-11-24 President And Fellows Of Harvard College Methods for detecting and identifying genomic nucleic acids
US10450599B2 (en) 2016-07-05 2019-10-22 California Institute Of Technology Fractional initiator hybridization chain reaction
WO2018183876A1 (en) 2017-03-31 2018-10-04 Ultivue, Inc. Dna-antigen exchange and amplification

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