MX370481B - Combinaciones de elementos de vector de expresión, métodos novedosos de generación de células de producción y su uso para la producción recombinante de polipéptidos. - Google Patents

Combinaciones de elementos de vector de expresión, métodos novedosos de generación de células de producción y su uso para la producción recombinante de polipéptidos.

Info

Publication number
MX370481B
MX370481B MX2017012736A MX2017012736A MX370481B MX 370481 B MX370481 B MX 370481B MX 2017012736 A MX2017012736 A MX 2017012736A MX 2017012736 A MX2017012736 A MX 2017012736A MX 370481 B MX370481 B MX 370481B
Authority
MX
Mexico
Prior art keywords
vectors containing
clones
signal sequence
polya signal
productivity
Prior art date
Application number
MX2017012736A
Other languages
English (en)
Inventor
Knoetgen Hendrik
Michael Huelsmann Peter
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Publication of MX370481B publication Critical patent/MX370481B/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2851—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the lectin superfamily, e.g. CD23, CD72
    • C07K16/2854—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the lectin superfamily, e.g. CD23, CD72 against selectins, e.g. CD62
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/46—Hybrid immunoglobulins
    • C07K16/468—Immunoglobulins having two or more different antigen binding sites, e.g. multifunctional antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/36—Vector systems having a special element relevant for transcription being a transcription termination element
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/42—Vector systems having a special element relevant for transcription being an intron or intervening sequence for splicing and/or stability of RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
    • C12N2840/203—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

En la presente se reporta que para transfecciones transitorias el uso del promotor alfa de factor 1 de elongación humano (con el intrón A) proporciona una productividad mejorada (en organización de LC-HC-SM), el uso de la secuencia de señal poliA de hormona del crecimiento bovina proporciona una productividad mejorada en comparación con el uso de la secuencia de señal poliA de SV40, la adición de hGT a la secuencia de señal poliA de bGH resulta en una productividad aumentada en vectores que contienen el promotor hCMV y la organización de vector CL (3'-5')-HC-SM resulta en expresión mejorada. Para acumulados estables se reporta que acumulados generados con vectores que contienen el promotor a de hEF1 muestran una productividad mejorada en análisis por lotes, clones generados con los vectores que contienen el promotor hEF1a muestran un número reducido de clones poco productores, y los clones generados con vectores que contienen el promotor hEF1a muestran una mayor estabilidad de expresión de IgG. Para clones únicos se reporta que la organización del vector con la posición corriente abajo del marcador de selección (LC-HC-SM) tiene un efecto positivo sobre la productividad de clones únicos y que los clones generados con vectores que contienen la secuencia de señal poliA de bGH y hGT tienen las mayores productividades.
MX2017012736A 2011-12-22 2012-12-19 Combinaciones de elementos de vector de expresión, métodos novedosos de generación de células de producción y su uso para la producción recombinante de polipéptidos. MX370481B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP11195361 2011-12-22
PCT/EP2012/076203 WO2013092743A2 (en) 2011-12-22 2012-12-19 Expression vector element combinations, novel production cell generation methods and their use for the recombinant production of polypeptides

Publications (1)

Publication Number Publication Date
MX370481B true MX370481B (es) 2019-12-16

Family

ID=47504950

Family Applications (2)

Application Number Title Priority Date Filing Date
MX2014007359A MX355624B (es) 2011-12-22 2012-12-19 Combinaciones de elementos de vector de expresion, metodos novedosos de generacion de celulas de produccion y su uso para la produccion recombinante de polipeptidos.
MX2017012736A MX370481B (es) 2011-12-22 2012-12-19 Combinaciones de elementos de vector de expresión, métodos novedosos de generación de células de producción y su uso para la producción recombinante de polipéptidos.

Family Applications Before (1)

Application Number Title Priority Date Filing Date
MX2014007359A MX355624B (es) 2011-12-22 2012-12-19 Combinaciones de elementos de vector de expresion, metodos novedosos de generacion de celulas de produccion y su uso para la produccion recombinante de polipeptidos.

Country Status (15)

Country Link
US (2) US20160208284A1 (es)
EP (2) EP3354660A1 (es)
JP (4) JP6096802B2 (es)
KR (4) KR102285184B1 (es)
CN (4) CN113896787A (es)
BR (1) BR112014014239B1 (es)
CA (2) CA3149402A1 (es)
ES (1) ES2930215T3 (es)
HK (1) HK1200848A1 (es)
HR (1) HRP20221383T1 (es)
MX (2) MX355624B (es)
PL (1) PL2794651T3 (es)
RU (2) RU2756910C2 (es)
SG (3) SG10201900915WA (es)
WO (1) WO2013092743A2 (es)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA3149402A1 (en) * 2011-12-22 2013-06-27 F. Hoffman-La Roche Ag Expression vector element combinations, novel production cell generation methods and their use for the recombinant production of polypeptides
EP3683314A1 (en) * 2013-10-07 2020-07-22 Prestige Biopharma Pte. Ltd. Bicistronic expression vector for antibody expression and method for producing antibody using same
CN105255867A (zh) * 2015-10-19 2016-01-20 北京益生合生物科技有限公司 一种防止启动子在传代培养中逐渐灭活的基因序列及其应用
US11098310B2 (en) 2016-01-27 2021-08-24 Just-Evotec Biologics, Inc. Expression from transposon-based vectors and uses
US11261462B2 (en) 2016-01-27 2022-03-01 Just-Evotec Biologics, Inc. Inducible expression from transposon-based vectors and uses
US11028410B2 (en) 2016-01-27 2021-06-08 Just-Evotec Biologics, Inc. Hybrid promoter and uses thereof
CN110022904B (zh) 2016-10-20 2024-04-19 桑格摩生物治疗股份有限公司 用于治疗法布里病的方法和组合物
JP7282689B2 (ja) * 2017-05-19 2023-05-29 カウンスィル オブ サイエンティフィック アンド インダストリアル リサーチ リフォールディングした組み換えヒト化ラニビズマブの製造方法
JP7081139B2 (ja) * 2017-12-20 2022-06-07 東ソー株式会社 新規プロモーターおよびそれを含む発現ベクター
BR112021005115A2 (pt) * 2018-09-20 2021-06-15 Sanofi métodos e composições de clonagem universal baseada em íntron
KR102559149B1 (ko) * 2018-10-26 2023-07-24 에프. 호프만-라 로슈 아게 재조합효소 매개 카세트 교환을 이용한 다중특이적 항체 스크리닝 방법
TW202237657A (zh) 2021-01-29 2022-10-01 美商默沙東藥廠 計畫性死亡受體1(pd-1)抗體之組合物及獲得該組合物之方法
KR102640929B1 (ko) 2023-06-02 2024-02-27 (주) 멥스젠 미세생체조직시스템 형성 장치

Family Cites Families (95)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4816567A (en) 1983-04-08 1989-03-28 Genentech, Inc. Recombinant immunoglobin preparations
EP0173552B1 (en) 1984-08-24 1991-10-09 The Upjohn Company Recombinant dna compounds and the expression of polypeptides such as tpa
US5168062A (en) 1985-01-30 1992-12-01 University Of Iowa Research Foundation Transfer vectors and microorganisms containing human cytomegalovirus immediate-early promoter-regulatory DNA sequence
US4676980A (en) 1985-09-23 1987-06-30 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Target specific cross-linked heteroantibodies
US6548640B1 (en) 1986-03-27 2003-04-15 Btg International Limited Altered antibodies
WO1988007089A1 (en) 1987-03-18 1988-09-22 Medical Research Council Altered antibodies
JP2919890B2 (ja) 1988-11-11 1999-07-19 メディカル リサーチ カウンスル 単一ドメインリガンド、そのリガンドからなる受容体、その製造方法、ならびにそのリガンドおよび受容体の使用
US5266491A (en) 1989-03-14 1993-11-30 Mochida Pharmaceutical Co., Ltd. DNA fragment and expression plasmid containing the DNA fragment
JP3051411B2 (ja) 1989-03-14 2000-06-12 持田製薬株式会社 新規ｄｎａならびにそれを含有する発現プラスミド
DE3920358A1 (de) 1989-06-22 1991-01-17 Behringwerke Ag Bispezifische und oligospezifische, mono- und oligovalente antikoerperkonstrukte, ihre herstellung und verwendung
US5959177A (en) 1989-10-27 1999-09-28 The Scripps Research Institute Transgenic plants expressing assembled secretory antibodies
US6075181A (en) 1990-01-12 2000-06-13 Abgenix, Inc. Human antibodies derived from immunized xenomice
US6150584A (en) 1990-01-12 2000-11-21 Abgenix, Inc. Human antibodies derived from immunized xenomice
US5770429A (en) 1990-08-29 1998-06-23 Genpharm International, Inc. Transgenic non-human animals capable of producing heterologous antibodies
CA2405246A1 (en) 1990-12-03 1992-06-11 Genentech, Inc. Enrichment method for variant proteins with alterred binding properties
US5571894A (en) 1991-02-05 1996-11-05 Ciba-Geigy Corporation Recombinant antibodies specific for a growth factor receptor
CA2103059C (en) 1991-06-14 2005-03-22 Paul J. Carter Method for making humanized antibodies
GB9114948D0 (en) 1991-07-11 1991-08-28 Pfizer Ltd Process for preparing sertraline intermediates
CA2116774C (en) 1991-09-19 2003-11-11 Paul J. Carter Expression in e. coli antibody fragments having at least a cysteine present as a free thiol. use for the production of bifunctional f(ab') 2 antibodies
US5587458A (en) 1991-10-07 1996-12-24 Aronex Pharmaceuticals, Inc. Anti-erbB-2 antibodies, combinations thereof, and therapeutic and diagnostic uses thereof
WO1993008829A1 (en) 1991-11-04 1993-05-13 The Regents Of The University Of California Compositions that mediate killing of hiv-infected cells
CA2129663C (en) 1992-02-06 2005-07-05 James S. Huston Biosynthetic binding protein for cancer marker
RU2201449C2 (ru) * 1994-10-14 2003-03-27 Сембайозис Дженетикс Инк. Слитый полипептид, способный к целенаправленному переносу к масляному телу, химерная днк-контрукция, экспрессирующая кассета
US5789199A (en) 1994-11-03 1998-08-04 Genentech, Inc. Process for bacterial production of polypeptides
US5731168A (en) 1995-03-01 1998-03-24 Genentech, Inc. Method for making heteromultimeric polypeptides
US5840523A (en) 1995-03-01 1998-11-24 Genetech, Inc. Methods and compositions for secretion of heterologous polypeptides
US5869046A (en) 1995-04-14 1999-02-09 Genentech, Inc. Altered polypeptides with increased half-life
GB9603256D0 (en) 1996-02-16 1996-04-17 Wellcome Found Antibodies
US5888809A (en) 1997-05-01 1999-03-30 Icos Corporation Hamster EF-1α transcriptional regulatory DNA
DE69830315T2 (de) 1997-06-24 2006-02-02 Genentech Inc., San Francisco Galactosylierte glykoproteine enthaltende zusammensetzungen und verfahren zur deren herstellung
US6040498A (en) 1998-08-11 2000-03-21 North Caroline State University Genetically engineered duckweed
WO1999022764A1 (en) 1997-10-31 1999-05-14 Genentech, Inc. Methods and compositions comprising glycoprotein glycoforms
US6610833B1 (en) 1997-11-24 2003-08-26 The Institute For Human Genetics And Biochemistry Monoclonal human natural antibodies
DK1034298T3 (da) 1997-12-05 2012-01-30 Scripps Research Inst Humanisering af murint antistof
ATE458007T1 (de) 1998-04-20 2010-03-15 Glycart Biotechnology Ag Glykosylierungs-engineering von antikörpern zur verbesserung der antikörperabhängigen zellvermittelten zytotoxizität
CA2704600C (en) 1999-04-09 2016-10-25 Kyowa Hakko Kirin Co., Ltd. A method for producing antibodies with increased adcc activity
US7125978B1 (en) 1999-10-04 2006-10-24 Medicago Inc. Promoter for regulating expression of foreign genes
DE60022369T2 (de) 1999-10-04 2006-05-18 Medicago Inc., Sainte Foy Verfahren zur regulation der transkription von fremden genen in gegenwart von stickstoff
JP4668498B2 (ja) 1999-10-19 2011-04-13 協和発酵キリン株式会社 ポリペプチドの製造方法
US20030180714A1 (en) 1999-12-15 2003-09-25 Genentech, Inc. Shotgun scanning
AU2001247616B2 (en) 2000-04-11 2007-06-14 Genentech, Inc. Multivalent antibodies and uses therefor
KR100408844B1 (ko) 2000-07-29 2003-12-06 한국산업기술평가원 동물세포 발현벡터
HU231090B1 (hu) 2000-10-06 2020-07-28 Kyowa Kirin Co., Ltd. Antitest-kompozíciót termelő sejt
US6946292B2 (en) 2000-10-06 2005-09-20 Kyowa Hakko Kogyo Co., Ltd. Cells producing antibody compositions with increased antibody dependent cytotoxic activity
US7064191B2 (en) 2000-10-06 2006-06-20 Kyowa Hakko Kogyo Co., Ltd. Process for purifying antibody
US6596541B2 (en) 2000-10-31 2003-07-22 Regeneron Pharmaceuticals, Inc. Methods of modifying eukaryotic cells
US7041870B2 (en) 2000-11-30 2006-05-09 Medarex, Inc. Transgenic transchromosomal rodents for making human antibodies
CA2838062C (en) 2001-08-03 2015-12-22 Roche Glycart Ag Antibody glycosylation variants having increased antibody-dependent cellular cytotoxicity
US7053202B2 (en) 2001-10-19 2006-05-30 Millennium Pharmaceuticals, Inc. Immunoglobulin DNA cassette molecules, monobody constructs, methods of production, and methods of use therefor
HUP0600342A3 (en) 2001-10-25 2011-03-28 Genentech Inc Glycoprotein compositions
EP1308517A1 (en) * 2001-10-31 2003-05-07 Aventis Pharmacueticals Products Inc. Vectors for expressing multiple transgenes
CN1585822A (zh) * 2001-11-16 2005-02-23 拜奥根Idec公司 抗体的多顺反子表达
US20040093621A1 (en) 2001-12-25 2004-05-13 Kyowa Hakko Kogyo Co., Ltd Antibody composition which specifically binds to CD20
BR0309145A (pt) 2002-04-09 2005-02-01 Kyowa Hakko Kogyo Kk Células das quais o genoma é modificado
WO2003084570A1 (fr) 2002-04-09 2003-10-16 Kyowa Hakko Kogyo Co., Ltd. Medicament contenant une composition d'anticorps appropriee au patient souffrant de polymorphisme fc$g(g)riiia
AU2003236020B2 (en) 2002-04-09 2009-03-19 Kyowa Hakko Kirin Co., Ltd. Cell with depression or deletion of the activity of protein participating in GDP-fucose transport
JPWO2003085118A1 (ja) 2002-04-09 2005-08-11 協和醗酵工業株式会社 抗体組成物の製造方法
JPWO2003084569A1 (ja) 2002-04-09 2005-08-11 協和醗酵工業株式会社 抗体組成物含有医薬
US20040259150A1 (en) 2002-04-09 2004-12-23 Kyowa Hakko Kogyo Co., Ltd. Method of enhancing of binding activity of antibody composition to Fcgamma receptor IIIa
EP1513879B1 (en) 2002-06-03 2018-08-22 Genentech, Inc. Synthetic antibody phage libraries
TWI335821B (en) 2002-12-16 2011-01-11 Genentech Inc Immunoglobulin variants and uses thereof
CN100383244C (zh) * 2003-01-07 2008-04-23 西福根有限公司 用于生产重组多克隆蛋白质的方法
US20050079574A1 (en) 2003-01-16 2005-04-14 Genentech, Inc. Synthetic antibody phage libraries
US20080241884A1 (en) 2003-10-08 2008-10-02 Kenya Shitara Fused Protein Composition
EP1705251A4 (en) 2003-10-09 2009-10-28 Kyowa Hakko Kirin Co Ltd PROCESS FOR PRODUCING ANTIBODY COMPOSITION BY RNA INHIBITION OF FUNCTION OF $ G (A) 1,6-FUCOSYLTRANSFERASE
WO2005044859A2 (en) 2003-11-05 2005-05-19 Glycart Biotechnology Ag Cd20 antibodies with increased fc receptor binding affinity and effector function
JPWO2005053742A1 (ja) 2003-12-04 2007-06-28 協和醗酵工業株式会社 抗体組成物を含有する医薬
AU2005230848B9 (en) 2004-03-31 2011-06-02 Genentech, Inc. Humanized anti-TGF-beta antibodies
US7785903B2 (en) 2004-04-09 2010-08-31 Genentech, Inc. Variable domain library and uses
ES2403055T3 (es) 2004-04-13 2013-05-13 F. Hoffmann-La Roche Ag Anticuerpos anti-P-selectina
SI2116605T1 (sl) * 2004-06-17 2012-07-31 Wyeth Llc Plazmid s tremi popolnimi transkripcijskimi enotami in imunogeni sestavki za induciranje imunskega odgovora na HIV
TW200732472A (en) * 2005-10-21 2007-09-01 Hoffmann La Roche Method for the recombinant expression of a polypeptide
EP2465870A1 (en) 2005-11-07 2012-06-20 Genentech, Inc. Binding polypeptides with diversified and consensus VH/VL hypervariable sequences
US20070237764A1 (en) 2005-12-02 2007-10-11 Genentech, Inc. Binding polypeptides with restricted diversity sequences
CA2651567A1 (en) 2006-05-09 2007-11-22 Genentech, Inc. Binding polypeptides with optimized scaffolds
US10118970B2 (en) 2006-08-30 2018-11-06 Genentech, Inc. Multispecific antibodies
CN101563460A (zh) * 2006-12-22 2009-10-21 弗·哈夫曼-拉罗切有限公司 选择方法
US20080226635A1 (en) 2006-12-22 2008-09-18 Hans Koll Antibodies against insulin-like growth factor I receptor and uses thereof
BRPI0809361A2 (pt) * 2007-03-30 2014-09-02 Abbott Lab Elementos de vetor de expressão recombinante (reves) para melhorar a expressão de proteínas recombinantes em células hospedeiras
EP1995309A1 (en) 2007-05-21 2008-11-26 Vivalis Recombinant protein production in avian EBx® cells
CN100592373C (zh) 2007-05-25 2010-02-24 群康科技(深圳)有限公司 液晶显示面板驱动装置及其驱动方法
CN101688222B (zh) * 2007-06-29 2013-03-06 弗·哈夫曼-拉罗切有限公司 启动子
US8227577B2 (en) 2007-12-21 2012-07-24 Hoffman-La Roche Inc. Bivalent, bispecific antibodies
US8242247B2 (en) 2007-12-21 2012-08-14 Hoffmann-La Roche Inc. Bivalent, bispecific antibodies
US20090162359A1 (en) 2007-12-21 2009-06-25 Christian Klein Bivalent, bispecific antibodies
US9266967B2 (en) 2007-12-21 2016-02-23 Hoffmann-La Roche, Inc. Bivalent, bispecific antibodies
DK2235064T3 (en) 2008-01-07 2016-01-11 Amgen Inc A process for the preparation of heterodimeric Fc molecules using electrostatic control effects
PL2329020T3 (pl) * 2008-08-28 2013-08-30 Novartis Ag Prezentacja izoform polipeptydu na powierzchni komórki poprzez nadczytywanie kodonu stop
GB0903207D0 (en) * 2009-02-25 2009-04-08 Ucb Pharma Sa Method for expressing multimeric proteins
MX2011010159A (es) 2009-04-02 2011-10-17 Roche Glycart Ag Anticuerpos multiespecificos que comprenden anticuerpos de longitud completa y fragmentos fab de cadena sencilla.
JP5616428B2 (ja) 2009-04-07 2014-10-29 ロシュ グリクアート アクチェンゲゼルシャフト 三価の二重特異性抗体
EP2435473B1 (en) 2009-05-27 2013-10-02 F.Hoffmann-La Roche Ag Tri- or tetraspecific antibodies
US9676845B2 (en) 2009-06-16 2017-06-13 Hoffmann-La Roche, Inc. Bispecific antigen binding proteins
US8703132B2 (en) 2009-06-18 2014-04-22 Hoffmann-La Roche, Inc. Bispecific, tetravalent antigen binding proteins
CA3149402A1 (en) * 2011-12-22 2013-06-27 F. Hoffman-La Roche Ag Expression vector element combinations, novel production cell generation methods and their use for the recombinant production of polypeptides

Also Published As

Publication number Publication date
HRP20221383T1 (hr) 2023-01-06
KR20200032240A (ko) 2020-03-25
JP6417436B2 (ja) 2018-11-07
RU2756910C2 (ru) 2021-10-06
CN113896787A (zh) 2022-01-07
BR112014014239B1 (pt) 2021-10-13
JP2017108753A (ja) 2017-06-22
KR102229491B1 (ko) 2021-03-18
JP2019030311A (ja) 2019-02-28
CN104011075A (zh) 2014-08-27
PL2794651T3 (pl) 2022-12-27
CN107119073A (zh) 2017-09-01
CA3149402A1 (en) 2013-06-27
EP3354660A1 (en) 2018-08-01
RU2639519C2 (ru) 2017-12-21
CN113881702A (zh) 2022-01-04
KR20190132702A (ko) 2019-11-28
KR102166824B1 (ko) 2020-10-19
JP2020054372A (ja) 2020-04-09
SG11201403443WA (en) 2014-07-30
HK1200848A1 (en) 2015-08-14
MX355624B (es) 2018-04-25
RU2017140661A3 (es) 2021-08-09
CN104011075B (zh) 2017-06-06
KR20140106585A (ko) 2014-09-03
WO2013092743A3 (en) 2013-10-03
KR102285184B1 (ko) 2021-08-03
JP6096802B2 (ja) 2017-03-15
SG10201700169PA (en) 2017-02-27
SG10201900915WA (en) 2019-03-28
US20160208284A1 (en) 2016-07-21
WO2013092743A2 (en) 2013-06-27
JP7096806B2 (ja) 2022-07-06
KR20210031002A (ko) 2021-03-18
RU2017140661A (ru) 2019-02-12
KR102048556B1 (ko) 2019-11-26
MX2014007359A (es) 2014-08-01
CA2854249A1 (en) 2013-06-27
RU2014129727A (ru) 2016-02-20
ES2930215T3 (es) 2022-12-07
CA2854249C (en) 2022-05-03
BR112014014239A2 (pt) 2017-12-26
US20210024952A1 (en) 2021-01-28
EP2794651B1 (en) 2022-09-21
JP2015500656A (ja) 2015-01-08
EP2794651A2 (en) 2014-10-29

Similar Documents

Publication Publication Date Title
MX370481B (es) Combinaciones de elementos de vector de expresión, métodos novedosos de generación de células de producción y su uso para la producción recombinante de polipéptidos.
FI3536797T3 (fi) Siirtogeenin tehostettu ilmentäminen ja prosessointi
HRP20170380T1 (hr) Životinje s humaniziranim il-5
WO2015042557A4 (en) Non-human animals having a humanized signal-regulatory protein gene
AR078544A1 (es) Procedimiento de cultivo de celulas de mamiferos para la produccion de proteinas
BR112014014913A2 (pt) mutante prongf e método de preparação de uma beta-ngf humana biologicamente ativa
MY157086A (en) Cells for producing recombinant iduronate-2-sulfatase
RU2015112607A (ru) Генетически модифицированные, не принадлежащие к человеческому роду животные и способ их использования
MX2015012361A (es) Metodos de fabricacion para controlar el contenido de lisina c-terminal, galactosa y acido sialico en proteinas recombinantes.
NZ733113A (en) Methods of producing long acting ctp-modified growth hormone polypeptides
SG10201901417UA (en) Production of proteins in glutamine-free cell culture media
HRP20200744T1 (hr) Organizacija ekspresijskog vektora, novi postupci generiranja proizvodnje stanica i njihova uporaba za rekombinantnu proizvodnju polipeptida
WO2007003582A3 (en) Serum-free stable transfection and production of recombinant human proteins in human cell lines
MX371007B (es) Vectores de expresión que comprenden un promotor de citomegalovirus quimérico y secuencias mejoradoras.
MY161552A (en) A polynucleotide and polypeptide sequence and methods thereof
NZ603196A (en) Methods of use of soluble cd24 for therapy of rheumatoid arthritis
JP2015516156A5 (es)
MX2019003256A (es) Vectores basados en el virus de la tristeza de los citricos para la expresion de gen/es ajenos.
CN104974226B (zh) 一种用于蛋白质表达的信号肽
WO2013032999A3 (en) Polydnavirus vectors
MX352229B (es) Metodo de produccion de polipeptido recombinante.
MX2013013937A (es) Anticuerpos del receptor eritropoyetina (epo) anti-humana y metodos de uso.
MX2015013842A (es) Metodos y constructos para expresar proteinas biologicamente activas en celulas de mamifero.
WO2020068014A3 (en) Tetrahymena thermophila artificial chromosome 1 (ttac1) and its use for the production of recombinant proteins
RU2013123459A (ru) Плазмида для экспрессии рекомбинантного фактора свертываемости крови viii человека с делетированным в-доменом, моноклональная линия клеток млекопитающего - продуцент рекомбинантного фактора свертываемости крови viii человека с делетированным в-доменом и способ получения указанного фактора