SG10201700169PA - Expression vector element combinations, novel production cell generation methods and their use for the recombinant production of polypeptides - Google Patents

Expression vector element combinations, novel production cell generation methods and their use for the recombinant production of polypeptides

Info

Publication number
SG10201700169PA
SG10201700169PA SG10201700169PA SG10201700169PA SG10201700169PA SG 10201700169P A SG10201700169P A SG 10201700169PA SG 10201700169P A SG10201700169P A SG 10201700169PA SG 10201700169P A SG10201700169P A SG 10201700169PA SG 10201700169P A SG10201700169P A SG 10201700169PA
Authority
SG
Singapore
Prior art keywords
polypeptides
expression vector
vector element
cell generation
generation methods
Prior art date
Application number
SG10201700169PA
Inventor
Peter Michael Huelsmann
Hendrik Knoetgen
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Publication of SG10201700169PA publication Critical patent/SG10201700169PA/en

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2851Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the lectin superfamily, e.g. CD23, CD72
    • C07K16/2854Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the lectin superfamily, e.g. CD23, CD72 against selectins, e.g. CD62
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/46Hybrid immunoglobulins
    • C07K16/468Immunoglobulins having two or more different antigen binding sites, e.g. multifunctional antibodies
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2317/00Immunoglobulins specific features
    • C07K2317/10Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14Specific host cells or culture conditions, e.g. components, pH or temperature
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00Vector systems having a special element relevant for transcription
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00Vector systems having a special element relevant for transcription
    • C12N2830/36Vector systems having a special element relevant for transcription being a transcription termination element
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00Vector systems having a special element relevant for transcription
    • C12N2830/42Vector systems having a special element relevant for transcription being an intron or intervening sequence for splicing and/or stability of RNA
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00Vectors comprising a special translation-regulating system
    • C12N2840/20Vectors comprising a special translation-regulating system translation of more than one cistron
    • C12N2840/203Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
SG10201700169PA 2011-12-22 2012-12-19 Expression vector element combinations, novel production cell generation methods and their use for the recombinant production of polypeptides SG10201700169PA (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP11195361 2011-12-22

Publications (1)

Publication Number Publication Date
SG10201700169PA true SG10201700169PA (en) 2017-02-27

Family

ID=47504950

Family Applications (3)

Application Number Title Priority Date Filing Date
SG10201700169PA SG10201700169PA (en) 2011-12-22 2012-12-19 Expression vector element combinations, novel production cell generation methods and their use for the recombinant production of polypeptides
SG10201900915WA SG10201900915WA (en) 2011-12-22 2012-12-19 Expression vector element combinations, novel production cell generation methods and their use for the recombinant production of polypeptides
SG11201403443WA SG11201403443WA (en) 2011-12-22 2012-12-19 Expression vector element combinations, novel production cell generation methods and their use for the recombinant production of polypeptides

Family Applications After (2)

Application Number Title Priority Date Filing Date
SG10201900915WA SG10201900915WA (en) 2011-12-22 2012-12-19 Expression vector element combinations, novel production cell generation methods and their use for the recombinant production of polypeptides
SG11201403443WA SG11201403443WA (en) 2011-12-22 2012-12-19 Expression vector element combinations, novel production cell generation methods and their use for the recombinant production of polypeptides

Country Status (15)

Country Link
US (2) US20160208284A1 (en)
EP (2) EP2794651B1 (en)
JP (4) JP6096802B2 (en)
KR (4) KR102229491B1 (en)
CN (4) CN113881702A (en)
BR (1) BR112014014239B1 (en)
CA (2) CA3149402A1 (en)
ES (1) ES2930215T3 (en)
HK (1) HK1200848A1 (en)
HR (1) HRP20221383T1 (en)
MX (2) MX370481B (en)
PL (1) PL2794651T3 (en)
RU (2) RU2756910C2 (en)
SG (3) SG10201700169PA (en)
WO (1) WO2013092743A2 (en)

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US11261462B2 (en) 2016-01-27 2022-03-01 Just-Evotec Biologics, Inc. Inducible expression from transposon-based vectors and uses
US11098310B2 (en) 2016-01-27 2021-08-24 Just-Evotec Biologics, Inc. Expression from transposon-based vectors and uses
EP3528852A4 (en) 2016-10-20 2020-06-03 Sangamo Therapeutics, Inc. Methods and compositions for the treatment of fabry disease
CN110636859B (en) * 2017-05-19 2024-05-03 科学与工业研究委员会 Methods for producing refolded recombinant humanized ranibizumab
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AU2019342866A1 (en) * 2018-09-20 2021-05-20 Sanofi Intron-based universal cloning methods and compositions
KR102559149B1 (en) * 2018-10-26 2023-07-24 에프. 호프만-라 로슈 아게 Multispecific antibody screening method using recombinase-mediated cassette exchange
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US20160208284A1 (en) 2016-07-21
MX2014007359A (en) 2014-08-01
CA2854249A1 (en) 2013-06-27
EP2794651B1 (en) 2022-09-21
BR112014014239A2 (en) 2017-12-26
RU2014129727A (en) 2016-02-20
JP2017108753A (en) 2017-06-22
CA3149402A1 (en) 2013-06-27
EP3354660A1 (en) 2018-08-01
KR102048556B1 (en) 2019-11-26
HRP20221383T1 (en) 2023-01-06
CN113896787A (en) 2022-01-07
KR20200032240A (en) 2020-03-25
HK1200848A1 (en) 2015-08-14
SG11201403443WA (en) 2014-07-30
WO2013092743A2 (en) 2013-06-27
RU2017140661A (en) 2019-02-12
SG10201900915WA (en) 2019-03-28
JP2019030311A (en) 2019-02-28
CA2854249C (en) 2022-05-03
BR112014014239B1 (en) 2021-10-13
JP2015500656A (en) 2015-01-08
MX355624B (en) 2018-04-25
KR20140106585A (en) 2014-09-03
CN107119073A (en) 2017-09-01
ES2930215T3 (en) 2022-12-07
MX370481B (en) 2019-12-16
JP2020054372A (en) 2020-04-09
KR102229491B1 (en) 2021-03-18
RU2756910C2 (en) 2021-10-06
CN104011075B (en) 2017-06-06
US20210024952A1 (en) 2021-01-28
RU2017140661A3 (en) 2021-08-09
JP6417436B2 (en) 2018-11-07
KR102166824B1 (en) 2020-10-19
CN104011075A (en) 2014-08-27
JP7096806B2 (en) 2022-07-06
KR20190132702A (en) 2019-11-28
PL2794651T3 (en) 2022-12-27
WO2013092743A3 (en) 2013-10-03
KR102285184B1 (en) 2021-08-03
KR20210031002A (en) 2021-03-18
CN113881702A (en) 2022-01-04
JP6096802B2 (en) 2017-03-15
RU2639519C2 (en) 2017-12-21
EP2794651A2 (en) 2014-10-29

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