MX365312B - Vectores basados en el virus de la tristeza de los cítricos para la expresión de gen/es ajenos. - Google Patents

Vectores basados en el virus de la tristeza de los cítricos para la expresión de gen/es ajenos.

Info

Publication number
MX365312B
MX365312B MX2014003508A MX2014003508A MX365312B MX 365312 B MX365312 B MX 365312B MX 2014003508 A MX2014003508 A MX 2014003508A MX 2014003508 A MX2014003508 A MX 2014003508A MX 365312 B MX365312 B MX 365312B
Authority
MX
Mexico
Prior art keywords
expression
foreign gene
based vectors
citrus tristeza
tristeza virus
Prior art date
Application number
MX2014003508A
Other languages
English (en)
Other versions
MX2014003508A (es
Inventor
William O Dawson
Svetlana Y Folimonova
Choaa El-Mohtar
Original Assignee
Univ Florida
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Florida filed Critical Univ Florida
Publication of MX2014003508A publication Critical patent/MX2014003508A/es
Publication of MX365312B publication Critical patent/MX365312B/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8203—Virus mediated transformation

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Virology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

La presente invención se refiere a un método de transfección de un árbol de cítricos con un gen de interés que tiene un extremo 5´y un extremo 3´, dicho método caracterizado porque comprende inocular dicho árbol de cítricos con una muestra que comprende por lo menos un vector del virus de la tristeza de los cítricos (CTV) para producir un árbol inoculado, dicho vector de CTV diseñado para comprender una construcción que comprende un gen heterólogo de interés y un elemento controlador heterólogo de ARN subgenómico (ARNsg) colocado río arriba del extremo 5´de dicho gen heterólogo de interés para controlar la expresión de dicho gen heterólogo de interés; y crecer dicho árbol inoculado bajo condiciones que permitan una infección sistémica de dicho árbol con dicho por lo menos un vector de CTV; en donde la expresión de dicho gen heterólogo de interés ocurre durante dos meses o más en el árbol con una mayoría de virus en la infección sistémica que comprende dicho vector de CTV.
MX2014003508A 2011-09-21 2012-09-21 Vectores basados en el virus de la tristeza de los cítricos para la expresión de gen/es ajenos. MX365312B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201161537154P 2011-09-21 2011-09-21
PCT/US2012/056565 WO2013044023A2 (en) 2011-09-21 2012-09-21 Citrus tristeza virus based vectors for foreign gene/s expression

Publications (2)

Publication Number Publication Date
MX2014003508A MX2014003508A (es) 2015-03-20
MX365312B true MX365312B (es) 2019-05-29

Family

ID=47915097

Family Applications (2)

Application Number Title Priority Date Filing Date
MX2014003508A MX365312B (es) 2011-09-21 2012-09-21 Vectores basados en el virus de la tristeza de los cítricos para la expresión de gen/es ajenos.
MX2019003256A MX2019003256A (es) 2011-09-21 2014-03-21 Vectores basados en el virus de la tristeza de los citricos para la expresion de gen/es ajenos.

Family Applications After (1)

Application Number Title Priority Date Filing Date
MX2019003256A MX2019003256A (es) 2011-09-21 2014-03-21 Vectores basados en el virus de la tristeza de los citricos para la expresion de gen/es ajenos.

Country Status (6)

Country Link
US (2) US10093939B2 (es)
CN (2) CN110734928B (es)
BR (1) BR112014006794A2 (es)
MX (2) MX365312B (es)
WO (1) WO2013044023A2 (es)
ZA (1) ZA201402793B (es)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110734928B (zh) * 2011-09-21 2023-08-04 佛罗里达大学研究基金会有限公司 使用柑橘衰退病毒载体进行外源基因表达
WO2015031130A1 (en) * 2013-08-28 2015-03-05 Southern Gardens Citrus Citrus plants resistant to citrus huanglongbing (ex greening) caused by candidatus liberibacter asiaticus (las) and bacterial canker caused by (xanthomonas axonopodis pv. citri) (xac)
US10557148B2 (en) * 2014-06-06 2020-02-11 Cornell University Compositions and methods for deterring feeding by psyllids
BR102017001164A2 (pt) 2016-01-26 2019-03-06 Embrapa - Empresa Brasileira De Pesquisa Agropecuária Composições de rna de fita dupla para controle de diaphorina citri e métodos de uso.
WO2021072358A1 (en) * 2019-10-10 2021-04-15 University Of Florida Research Foundation, Incorporated Ctv-based rna replicon transferable to multiple cells and tissues in citrus

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009099877A2 (en) * 2008-01-31 2009-08-13 State Of Oregon Acting By And Through The State Board Of Higher Educ. On Behalf Of Oregon State University Closterovirus vectors and methods
US8629334B2 (en) * 2008-07-16 2014-01-14 University Of Florida Research Foundation, Inc. Viral-based transient-expression vector system for trees
ES2363325B1 (es) 2009-11-18 2012-06-04 Instituto Valenciano De Investigaciones Agrarias Método para conseguir resistencia frente a enfermedades de los cítricos causadas por insectos, por hongos u omicetos o por bacterias o nematodos.
US8389804B2 (en) * 2010-04-08 2013-03-05 University Of Florida Research Foundation, Inc. Viral based transient-expression vector system that allows multiple applications
CN110734928B (zh) * 2011-09-21 2023-08-04 佛罗里达大学研究基金会有限公司 使用柑橘衰退病毒载体进行外源基因表达
US10017747B2 (en) * 2011-09-21 2018-07-10 University Of Florida Research Foundation, Inc. Citrus tristeza virus based vectors for foreign gene/s expression

Also Published As

Publication number Publication date
US10851381B2 (en) 2020-12-01
WO2013044023A3 (en) 2013-06-27
MX2019003256A (es) 2021-11-16
US20130125254A1 (en) 2013-05-16
CN105939599A (zh) 2016-09-14
CN105939599B (zh) 2019-10-18
US10093939B2 (en) 2018-10-09
CN110734928B (zh) 2023-08-04
MX2014003508A (es) 2015-03-20
US20180030461A9 (en) 2018-02-01
WO2013044023A2 (en) 2013-03-28
BR112014006794A2 (pt) 2017-11-21
ZA201402793B (en) 2015-09-30
CN110734928A (zh) 2020-01-31
US20190211345A1 (en) 2019-07-11

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