WO2011127340A3 - Viral-based transient-expression vector system that allows multiple applications - Google Patents

Viral-based transient-expression vector system that allows multiple applications Download PDF

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Publication number
WO2011127340A3
WO2011127340A3 PCT/US2011/031671 US2011031671W WO2011127340A3 WO 2011127340 A3 WO2011127340 A3 WO 2011127340A3 US 2011031671 W US2011031671 W US 2011031671W WO 2011127340 A3 WO2011127340 A3 WO 2011127340A3
Authority
WO
WIPO (PCT)
Prior art keywords
viral
multiple applications
vectors
expression vector
vector system
Prior art date
Application number
PCT/US2011/031671
Other languages
French (fr)
Other versions
WO2011127340A2 (en
Inventor
O. Dawson William
Folimonova Svetlana
Original Assignee
University Of Florida Research Foundation, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University Of Florida Research Foundation, Inc. filed Critical University Of Florida Research Foundation, Inc.
Priority to CN201180027897.1A priority Critical patent/CN102933713B/en
Priority to ES201290074A priority patent/ES2477141B2/en
Priority to US13/207,731 priority patent/US8389804B2/en
Publication of WO2011127340A2 publication Critical patent/WO2011127340A2/en
Publication of WO2011127340A3 publication Critical patent/WO2011127340A3/en
Priority to ZA2012/08259A priority patent/ZA201208259B/en
Priority to US13/761,845 priority patent/US9611483B2/en
Priority to US15/477,563 priority patent/US10472641B2/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8203—Virus mediated transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8283—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for virus resistance

Abstract

Disclosed herein are viral vectors suitable for transfection into woody trees for purposes of delivering and expressing beneficial genes. Specifically exemplified herein are vectors for transfecting citrus trees. The vectors allow for the expression of useful proteins, such as those that can protect the tree from disease. Specifically exemplified herein are methods of transfecting woody trees that allow multiple applications of vectors while avoiding superinfection exclusion.
PCT/US2011/031671 2010-04-08 2011-04-08 Viral-based transient-expression vector system that allows multiple applications WO2011127340A2 (en)

Priority Applications (6)

Application Number Priority Date Filing Date Title
CN201180027897.1A CN102933713B (en) 2010-04-08 2011-04-08 Viral-based transient-expression vector system that allows multiple applications
ES201290074A ES2477141B2 (en) 2010-04-08 2011-04-08 SYSTEM BASED ON A VIRAL TRANSITIONAL EXPRESSION VECTOR THAT ALLOWS MULTIPLE APPLICATIONS
US13/207,731 US8389804B2 (en) 2010-04-08 2011-08-11 Viral based transient-expression vector system that allows multiple applications
ZA2012/08259A ZA201208259B (en) 2010-04-08 2012-11-05 Viral-based transient-expression vector system that allows multiple applications
US13/761,845 US9611483B2 (en) 2010-04-08 2013-02-07 Viral based transient-expression vector system that allows multiple applications
US15/477,563 US10472641B2 (en) 2010-04-08 2017-04-03 Viral based transient-expression vector system that allows multiple applications

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US32197010P 2010-04-08 2010-04-08
US61/321,970 2010-04-08
US201161440445P 2011-02-08 2011-02-08
US61/440,445 2011-02-08

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US13/207,731 Continuation US8389804B2 (en) 2010-04-08 2011-08-11 Viral based transient-expression vector system that allows multiple applications

Publications (2)

Publication Number Publication Date
WO2011127340A2 WO2011127340A2 (en) 2011-10-13
WO2011127340A3 true WO2011127340A3 (en) 2012-08-09

Family

ID=44763557

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2011/031671 WO2011127340A2 (en) 2010-04-08 2011-04-08 Viral-based transient-expression vector system that allows multiple applications

Country Status (5)

Country Link
CN (2) CN102933713B (en)
BR (1) BRPI1101853A2 (en)
ES (1) ES2477141B2 (en)
WO (1) WO2011127340A2 (en)
ZA (1) ZA201208259B (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2015031130A1 (en) * 2013-08-28 2015-03-05 Southern Gardens Citrus Citrus plants resistant to citrus huanglongbing (ex greening) caused by candidatus liberibacter asiaticus (las) and bacterial canker caused by (xanthomonas axonopodis pv. citri) (xac)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040029280A1 (en) * 1997-03-14 2004-02-12 Selective Genetics, Inc. Viral vectors with modified tropism
US20040087537A1 (en) * 2002-09-19 2004-05-06 Molmed Spa Compositions comprising fusion polypeptides HIV-Nef and NGF, and methods of using the same
WO2009099877A2 (en) * 2008-01-31 2009-08-13 State Of Oregon Acting By And Through The State Board Of Higher Educ. On Behalf Of Oregon State University Closterovirus vectors and methods

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1500394A1 (en) * 2003-07-22 2005-01-26 Polichem S.A. Pharmaceutical compositions comprising ascorbic acid or the treatment of fungal superinfections and fungal recurrences
JP5992654B2 (en) * 2007-12-21 2016-09-14 ニュートリー株式会社 Composition for intestinal regulation and / or improvement of bowel movement
CN101270395A (en) * 2008-05-08 2008-09-24 重庆大学 Real time fluorescence reverse transcription polyase chain reaction solid phase reagent kit with one-step method of citrus decline virus and testing method thereof

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040029280A1 (en) * 1997-03-14 2004-02-12 Selective Genetics, Inc. Viral vectors with modified tropism
US20040087537A1 (en) * 2002-09-19 2004-05-06 Molmed Spa Compositions comprising fusion polypeptides HIV-Nef and NGF, and methods of using the same
WO2009099877A2 (en) * 2008-01-31 2009-08-13 State Of Oregon Acting By And Through The State Board Of Higher Educ. On Behalf Of Oregon State University Closterovirus vectors and methods

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
J. D. CHILDS.: "Superinfection Exclusion by Incomplete Genomes of Bacteriophage T4.", JOURNAL OF VIROLOGY., vol. 11, no. 1, 1973, pages 1 - 8 *
MARKUS U EHRENGRUBER ET AL.: "Semliki Forest virus vectors with mutations in the nonstructural protein 2 gene permit extended superinfection of neuronal and non-neuronal cells.", JOURNAL OF NEUROVIROLOGY., vol. 13, no. 4, 2007, pages 353 - 363 *
SVETLANA Y. FOLIMONOVA ET AL.: "Infection with Strains of Citrus Tristeza Virus Does Not Exclude Superinfection by Other Strains of the Virus.", JOURNAL OF VIROLOGY., vol. 84, no. 3, February 2010 (2010-02-01), pages 1314 - 1325, XP055072179, DOI: doi:10.1128/JVI.02075-09 *

Also Published As

Publication number Publication date
ES2477141B2 (en) 2015-02-23
CN102933713B (en) 2015-05-13
WO2011127340A2 (en) 2011-10-13
ES2477141R1 (en) 2014-08-06
CN105087638B (en) 2019-07-12
ES2477141A2 (en) 2014-07-15
ZA201208259B (en) 2013-07-31
CN105087638A (en) 2015-11-25
BRPI1101853A2 (en) 2015-08-11
CN102933713A (en) 2013-02-13

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