KR950703648A - 바이오틴의 생물공학적 제조방법[biotechnological method of producing biotin) - Google Patents
바이오틴의 생물공학적 제조방법[biotechnological method of producing biotin) Download PDFInfo
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- KR950703648A KR950703648A KR1019950701255A KR19950701255A KR950703648A KR 950703648 A KR950703648 A KR 950703648A KR 1019950701255 A KR1019950701255 A KR 1019950701255A KR 19950701255 A KR19950701255 A KR 19950701255A KR 950703648 A KR950703648 A KR 950703648A
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- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 title claims abstract description 30
- 229960002685 biotin Drugs 0.000 title claims abstract description 15
- 235000020958 biotin Nutrition 0.000 title claims abstract description 15
- 239000011616 biotin Substances 0.000 title claims abstract description 15
- 238000010352 biotechnological method Methods 0.000 title claims 2
- 239000013612 plasmid Substances 0.000 claims abstract description 9
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 9
- 101150076754 bioA gene Proteins 0.000 claims abstract description 7
- 101150023452 bioD gene Proteins 0.000 claims abstract description 7
- 101100381793 Bacillus subtilis (strain 168) bioK gene Proteins 0.000 claims abstract description 5
- 230000015572 biosynthetic process Effects 0.000 claims abstract description 3
- 239000012634 fragment Substances 0.000 claims abstract 13
- 230000002068 genetic effect Effects 0.000 claims abstract 4
- 238000004519 manufacturing process Methods 0.000 claims abstract 4
- 244000005700 microbiome Species 0.000 claims abstract 4
- 230000035772 mutation Effects 0.000 claims abstract 4
- 101150029327 bioB gene Proteins 0.000 claims abstract 3
- 101100218845 Escherichia coli (strain K12) bioH gene Proteins 0.000 claims abstract 2
- 101150085692 bioC gene Proteins 0.000 claims abstract 2
- 101150032820 bioF gene Proteins 0.000 claims abstract 2
- 230000002103 transcriptional effect Effects 0.000 claims abstract 2
- 241000588724 Escherichia coli Species 0.000 claims 9
- 238000006243 chemical reaction Methods 0.000 claims 4
- 108010057366 Flavodoxin Proteins 0.000 claims 3
- 210000004671 cell-free system Anatomy 0.000 claims 3
- 238000000034 method Methods 0.000 claims 3
- 235000018102 proteins Nutrition 0.000 claims 3
- 102000004169 proteins and genes Human genes 0.000 claims 3
- MTCFGRXMJLQNBG-REOHCLBHSA-N (2S)-2-Amino-3-hydroxypropansäure Chemical compound OC[C@H](N)C(O)=O MTCFGRXMJLQNBG-REOHCLBHSA-N 0.000 claims 2
- 241000589158 Agrobacterium Species 0.000 claims 2
- DCXYFEDJOCDNAF-UHFFFAOYSA-N Asparagine Natural products OC(=O)C(N)CC(N)=O DCXYFEDJOCDNAF-UHFFFAOYSA-N 0.000 claims 2
- 241000588921 Enterobacteriaceae Species 0.000 claims 2
- DCXYFEDJOCDNAF-REOHCLBHSA-N L-asparagine Chemical compound OC(=O)[C@@H](N)CC(N)=O DCXYFEDJOCDNAF-REOHCLBHSA-N 0.000 claims 2
- CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 claims 2
- ZDXPYRJPNDTMRX-VKHMYHEASA-N L-glutamine Chemical compound OC(=O)[C@@H](N)CCC(N)=O ZDXPYRJPNDTMRX-VKHMYHEASA-N 0.000 claims 2
- MEFKEPWMEQBLKI-AIRLBKTGSA-N S-adenosyl-L-methioninate Chemical compound O[C@@H]1[C@H](O)[C@@H](C[S+](CC[C@H](N)C([O-])=O)C)O[C@H]1N1C2=NC=NC(N)=C2N=C1 MEFKEPWMEQBLKI-AIRLBKTGSA-N 0.000 claims 2
- MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 claims 2
- XJLXINKUBYWONI-DQQFMEOOSA-N [[(2r,3r,4r,5r)-5-(6-aminopurin-9-yl)-3-hydroxy-4-phosphonooxyoxolan-2-yl]methoxy-hydroxyphosphoryl] [(2s,3r,4s,5s)-5-(3-carbamoylpyridin-1-ium-1-yl)-3,4-dihydroxyoxolan-2-yl]methyl phosphate Chemical compound NC(=O)C1=CC=C[N+]([C@@H]2[C@H]([C@@H](O)[C@H](COP([O-])(=O)OP(O)(=O)OC[C@@H]3[C@H]([C@@H](OP(O)(O)=O)[C@@H](O3)N3C4=NC=NC(N)=C4N=C3)O)O2)O)=C1 XJLXINKUBYWONI-DQQFMEOOSA-N 0.000 claims 2
- 229960001570 ademetionine Drugs 0.000 claims 2
- 235000001014 amino acid Nutrition 0.000 claims 2
- 229940024606 amino acid Drugs 0.000 claims 2
- 150000001413 amino acids Chemical class 0.000 claims 2
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical class N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims 2
- 235000009582 asparagine Nutrition 0.000 claims 2
- 229960001230 asparagine Drugs 0.000 claims 2
- 235000003704 aspartic acid Nutrition 0.000 claims 2
- OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 claims 2
- 210000004027 cell Anatomy 0.000 claims 2
- 235000018417 cysteine Nutrition 0.000 claims 2
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 claims 2
- 239000000284 extract Substances 0.000 claims 2
- 235000004554 glutamine Nutrition 0.000 claims 2
- ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 claims 2
- 150000002500 ions Chemical class 0.000 claims 2
- 229930027945 nicotinamide-adenine dinucleotide Natural products 0.000 claims 2
- 239000002244 precipitate Substances 0.000 claims 2
- 235000004400 serine Nutrition 0.000 claims 2
- 241000894007 species Species 0.000 claims 2
- 229960002363 thiamine pyrophosphate Drugs 0.000 claims 2
- 235000008170 thiamine pyrophosphate Nutrition 0.000 claims 2
- 239000011678 thiamine pyrophosphate Substances 0.000 claims 2
- YXVCLPJQTZXJLH-UHFFFAOYSA-N thiamine(1+) diphosphate chloride Chemical compound [Cl-].CC1=C(CCOP(O)(=O)OP(O)(O)=O)SC=[N+]1CC1=CN=C(C)N=C1N YXVCLPJQTZXJLH-UHFFFAOYSA-N 0.000 claims 2
- 238000013518 transcription Methods 0.000 claims 2
- 230000035897 transcription Effects 0.000 claims 2
- 101710117026 Biotin synthase Proteins 0.000 claims 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims 1
- 241000588923 Citrobacter Species 0.000 claims 1
- 241000588722 Escherichia Species 0.000 claims 1
- XUJNEKJLAYXESH-REOHCLBHSA-N L-Cysteine Chemical compound SC[C@H](N)C(O)=O XUJNEKJLAYXESH-REOHCLBHSA-N 0.000 claims 1
- 241000607142 Salmonella Species 0.000 claims 1
- 230000001851 biosynthetic effect Effects 0.000 claims 1
- 229910052799 carbon Inorganic materials 0.000 claims 1
- 230000002085 persistent effect Effects 0.000 claims 1
- 210000003705 ribosome Anatomy 0.000 claims 1
- 238000003786 synthesis reaction Methods 0.000 claims 1
- 241000305071 Enterobacterales Species 0.000 abstract 1
- 108020004705 Codon Proteins 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108091081024 Start codon Proteins 0.000 description 1
- 238000003776 cleavage reaction Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000037353 metabolic pathway Effects 0.000 description 1
- 230000007017 scission Effects 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
- C12P17/18—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms containing at least two hetero rings condensed among themselves or condensed with a common carbocyclic ring system, e.g. rifamycin
- C12P17/185—Heterocyclic compounds containing sulfur atoms as ring hetero atoms in the condensed system
- C12P17/186—Heterocyclic compounds containing sulfur atoms as ring hetero atoms in the condensed system containing a 2-oxo-thieno[3,4-d]imidazol nucleus, e.g. Biotin
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
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Abstract
본 발명은 유전자들이 전사 단위체에 배열되어있는, 장내세균으로부터의 바이오틴 생합성에 관여할 수 있는 바이오B, 바이오F, 바이오C, 바이오D 및 바이오A를 포함하는 DNA단편 또는 그의 동일한 기능의 유전적 변종 및 돌연변이에 관한 것이다. 본 발명은 또한 상기 DNA단편 및 플라스미드를 함유하는 미생물, 및 상기 미생물을 사용하는 바이오틴 제조방법에 관한 것이다.
Description
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 바이오틴 생합성의 대사 경로의 효소를 나타낸다.
제2도는 플라스미드 pBO30의 구조도를 나타낸다.
제3도는 플라스미드 pBO30, pBO30-9 및 pBO30A-15의 바이오D 유전자의 3'말단 부분 및 바이오A 유전자의 5'말단(파선 화살표;바이오A 출발 코돈을 밑줄긋고, 바이오D 정지 코돈은 점선으로 나타낸다) 대한 DNA 서열과 함께 플라스미드 구조에서 상대적인 제한 효소의 절단위치, 및 바이오A 유전자의 샤인-달가노(Shine-Dalgano, SD)서열을 나타낸다. 잠재적인 "스템-루프"구조는 실선 화살표로 표시한다.
Claims (23)
- 유전자들이 전사 단위체에 배열되어있는, 장내세군으로부터의 바이오틴 생합성 유전자인 바이오B, 바이오F, 바이오C, 바이오D 및 바이오A를 함유하는 DNA단편 또는 그의 동일한 기능의 유전적 변종 및 돌연변이.
- 제1항에 있어서, 장내세균이 에쉐리키아소, 살모넬라속 및 시트로박터속으로 구성된 군으로부터 선택된 DNA단편.
- 제1항 또는 제2항에 있어서, 장내세균이 에쉐리키아 콜라이(Escherichia coli)종으로부터 선택된 DNA단편.
- 제1항 내지 제3항중의 어느 한 항에 있어서, 전사 단위체의 유전자-조절인자가 tac프로모터를 포함하는 DNA단편.
- 제1항 내지 제4항중의 어느 한 항에 있어서, 바이오B 유전자에 직접 연관된 유전자-조절인자가 하기의 서열을 포함하는 DNA단편 : AAGCTTACTC CCCATCCCCC TGTTGACAAT TAATCATCGG CTCGTATAAT GTGTGGAATT GTGAGCGGAT AACAATTTCA CACAGGAAAC AGGATCGGTA CCTTAGGAGG TGACTAGTC.
- 제1항 내지 제4항중의 어느 한 항에 있어서, 바이오B 유전자에 직접 연관된 유전자-조절인자가 하기의 서열을 포함하는 DNA단편: AAGCTTACTC CCCATCCCCC TGTTGACAAT TAATCATCGG CTCGTATAAT GTGTGGAATT GTGAGCGGAT AACAATTTCA CACAGGAAAC AGGATCGGTA CCTAAGGAGG TTTACTAGTC.
- 제1항 내지 제4항중의 어느 한 항에 있어서, 바이오B 유전자에 직접 연관된 유전자-조절인자가 하기의 서열을 포함하는 DNA단편: AAGCTTACTC CCCATCCCCC TGTTGACAAT TAATCATCGG CTCGTATAAT GTGTGGAATT GTGAGCGGAT AACAATTTCA CACAGGAAAC AGGATCGGTA CCTAAGGAGA CTAGTC.
- 제1항 내지 제7항중의 어느 한 항에 있어서, 전사 단위체에서 지속성인 바이오D 및 바이오A 유전자 사이의 거리가 50bp이하인 DNA단편.
- 제1항 내지 제8항중의 어느 한 항에 있어서, 바이오D 및 바이오A 유전자가 바이오D 유전자의 3' 말단이 바이오A 유전자의 리보좀 견합부위를 포함하도록 배열된 DNA 단편.
- 제1항 내지 제9항중의 어느 한 항에 기재된 DNA 단편을 함유하는 플라스미드.
- 수탁번호가 각각 DSM 7246, DSM 7247 및 DSM 8554로 기탁된 에쉐리키아 콜라이 XL1-블루(Blue), 에쉐리키아 콜라이 BM4062 또는 에쉐리키아 콜라이 ED8767 내의 플라스미드 pBO30A-15/9.
- 수탁번호 DSM 8555로 기탁된 애그로박테리움/리조비움종 HK4 내의 폴라스미드 pBO47.
- 제1항 내지 제12 항중의 어느 한 항에 기재된 DNA 단편 또는 플라스미드를 함유한 미생물.
- 수탁번호가 각각 DSM 7246, DSM 7247 및 DSM 8554로 기탁된, 플라스미드 pBO30A-13/9를 각기 함유하는, 에쉐리키아 콜라이 XL1-블루, 에쉐리키아 콜라이 BM4062 및 에쉐리키아 콜라이 ED8767 및 그의 유전적 변종 및 돌연변이.
- 수탁번호 DSM 8555로 기탁된, 플라스미드 pB047을 함유하는, 애그로박테리움/리조비움종 HK4 및 그의 유전적 변종 및 돌연변이.
- 대사될 수 있는 탄소원이 제13항 내지 제15항중의 어느 한 항에 기재된 미생물에 의해 바이오틴으로 발효되는 바이오틴합성을 위한 생물공학적 방법.
- 전환이 티아민 피로포스페이트, NADPH, S-아데노실메티오닌, Fe2+이온, 시스테인, 및 아스파라긴, 아스파르트산, 글루타민 및 세린으로 구성된 군으로부터의 하나 이상의 다른 아미노산의 존재하에서 수행되는, 세포-프르 시스템에서의 바이오틴 합성효소에 의한 탈티오바이오 틴에서 바이오틴으로의 전환을 포함하는 바이오틴 제조방법.
- 제17항에 있어서, 전환이 플라보독신 및 페레독신(플라보독신)-NADP+리덕타제의 존재하에서 수행되는 바이오틴 제조방법.
- 제17항 또는 제18항에 있어서, 전환이 에쉐리키아 콜라이의 세포 추출물의 45% 포화된 황산암모늄 침전물에 의해 수득될 수 있는 단백질 분획의 존재하에서 수행되는 바이오틴 제조방법.
- 에쉐리키아 콜라이의 세포 주출물의 45% 포화된 황산암모늄 침전물에 의해 수득될 수 있는, 바이오틴 합성효소-촉진 작용을 갖는 단백질 분획.
- 세포-프리 시스템에서의 바이오틴 제조방법에 있어서 제20항에 기재된 단백질 분획의 용도.
- 세포-프리 시스템에서의 바이오틴 제조방법에 있어서, 플라보독신 및 페레독신(플라보도신)-NADP+리덕타제의 용도.
- 세포-프리 시스템에서의 바이오틴 제조방법에 있어서, 단독으로 또는 조합된, 티아민 피로포스페이트 및/또는 NADPH 및/또는 S-아데노실메티오닌 및/또는 Fe+이온 및/또는 시스테인 및/또는 아스파라긴 아스파르트산, 글루타민 및 세린으로 구성된 군으로부터의 하나 이상의 다른 아미노산의 용도.※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CH92-3124 | 1992-10-02 | ||
| CH312492 | 1992-10-02 | ||
| CH93-2134 | 1993-07-15 | ||
| CH213493 | 1993-07-15 | ||
| PCT/EP1993/002688 WO1994008023A2 (de) | 1992-10-02 | 1993-10-01 | Biotechnologisches verfahren zur herstellung von biotin |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| KR950703648A true KR950703648A (ko) | 1995-09-20 |
| KR100200542B1 KR100200542B1 (ko) | 1999-06-15 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1019950701255A KR100200542B1 (ko) | 1992-10-02 | 1993-10-01 | 바이오틴의 생물공학적 제조방법 |
Country Status (16)
| Country | Link |
|---|---|
| US (1) | US6083712A (ko) |
| EP (2) | EP0667909B1 (ko) |
| JP (2) | JP3566287B2 (ko) |
| KR (1) | KR100200542B1 (ko) |
| AT (1) | ATE163198T1 (ko) |
| AU (1) | AU4820293A (ko) |
| CA (1) | CA2145400C (ko) |
| CZ (1) | CZ285533B6 (ko) |
| DE (1) | DE59308149D1 (ko) |
| DK (1) | DK0667909T3 (ko) |
| ES (1) | ES2112995T3 (ko) |
| FI (1) | FI117515B (ko) |
| HU (1) | HU219827B (ko) |
| PL (2) | PL177635B1 (ko) |
| SK (1) | SK279669B6 (ko) |
| WO (1) | WO1994008023A2 (ko) |
Families Citing this family (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5859335A (en) * | 1994-12-08 | 1999-01-12 | Novartis Finance Corporation | Enhanced biotin biosynthesis in plant tissue |
| US5869719A (en) * | 1995-03-08 | 1999-02-09 | Novartis Finance Corporation | Transgenic plants having increased biotin content |
| WO1997016560A1 (en) * | 1995-11-02 | 1997-05-09 | Takeda Chemical Industries, Ltd. | Microorganism resistant to threonine analogue and production of biotin |
| ES2201219T3 (es) | 1996-05-06 | 2004-03-16 | F. Hoffmann-La Roche Ag | Produccion fermentativa de biotina. |
| DE69731451T2 (de) | 1996-09-27 | 2005-11-24 | Dsm Ip Assets B.V. | Biotin-Biosynthese-Gene II |
| US6737256B2 (en) | 1997-07-14 | 2004-05-18 | Roche Vitamins Inc. | Overcoming DAPA aminotransferase bottlenecks in biotin vitamers biosynthesis |
| DE19731274A1 (de) | 1997-07-22 | 1999-01-28 | Basf Ag | Verfahren zur Herstellung von Biotin |
| IL135776A0 (en) * | 1997-10-24 | 2001-05-20 | Life Technologies Inc | Recombinational cloning using nucleic acids having recombination sites |
| CA2384496A1 (en) * | 1999-09-08 | 2001-03-15 | Xenogen Corporation | Luciferase expression cassettes and methods of use |
| US6660907B2 (en) | 2000-07-10 | 2003-12-09 | Pioneer Hi-Bred International, Inc. | Genes encoding SCIP-1 orthologs and methods of use |
| US7198924B2 (en) | 2000-12-11 | 2007-04-03 | Invitrogen Corporation | Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites |
| WO2002090596A1 (en) * | 2001-05-07 | 2002-11-14 | Paradigm Genetics, Inc. | Methods for the identification of inhibitors of biotim synthase expression or activity in plants |
| WO2005054438A2 (en) | 2003-12-01 | 2005-06-16 | Invitrogen Corporation | Nucleic acid molecules containing recombination sites and methods of using the same |
| AU2009224600A1 (en) * | 2008-03-14 | 2009-09-17 | Merck Serono S.A. | Variation of recombinant expression titres by optimising bacterial ribosome binding sites |
| GB0920775D0 (en) * | 2009-11-26 | 2010-01-13 | King S College | Cells |
| EP3488006B1 (en) | 2016-07-25 | 2023-06-07 | INVISTA Textiles (U.K.) Limited | Materials and methods utilizing biotin producing mutant hosts for the production of 7-carbon chemicals |
| KR20230070943A (ko) * | 2021-11-15 | 2023-05-23 | 씨제이제일제당 (주) | 바이오틴 신타제 활성을 갖는 폴리펩티드 변이체 및 이를 이용한 바이오틴 생산 방법 |
Family Cites Families (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0740922B2 (ja) * | 1985-03-05 | 1995-05-10 | 株式会社資生堂 | ビオチン生産性微生物 |
| US5110731A (en) * | 1985-08-26 | 1992-05-05 | Amgen, Inc. | System for biotin synthesis |
| NZ217336A (en) * | 1985-08-26 | 1988-06-30 | Amgen | Production of biotin from microorganisms |
| JPS62155081A (ja) * | 1985-12-27 | 1987-07-10 | Shiseido Co Ltd | 新規微生物およびそれを用いる醗酵法によるビオチンの製造法 |
| AU616380B2 (en) * | 1986-09-30 | 1991-10-31 | Transgene S.A. | Cloning of the bioA, bioD, bioF, bioC, and bioH genes of Bacillus sphaericus, vectors and transformed cells and method for preparing biotin |
| CA1322735C (en) * | 1987-11-07 | 1993-10-05 | Shinichiro Haze | Microorganism having low acetate forming capability, and process for production of useful substrate using same |
| GB2216530B (en) * | 1988-03-22 | 1992-07-08 | Mini Agriculture & Fisheries | Genetic material for expression of biotin synthetase enzymes |
| JP3006847B2 (ja) * | 1990-03-27 | 2000-02-07 | 株式会社資生堂 | 新規微生物およびそれを用いるビオチン類の製造方法 |
| JPH04169180A (ja) * | 1990-11-02 | 1992-06-17 | Shiseido Co Ltd | 新規微生物及びそれを用いるビオチン活性物質の製造方法 |
| JP3290473B2 (ja) * | 1991-09-13 | 2002-06-10 | 株式会社資生堂 | ビオチンオペロン |
| JP3192704B2 (ja) * | 1991-10-17 | 2001-07-30 | 株式会社資生堂 | デスチオビオチンからビオチンへの変換方法 |
| US5374543A (en) * | 1992-10-02 | 1994-12-20 | Amgen Inc. | Enhanced indole biosynthesis |
| US5445952A (en) * | 1993-01-22 | 1995-08-29 | Basf Aktiengesellschaft | Method to produce biotin |
-
1993
- 1993-10-01 DE DE59308149T patent/DE59308149D1/de not_active Expired - Lifetime
- 1993-10-01 KR KR1019950701255A patent/KR100200542B1/ko not_active IP Right Cessation
- 1993-10-01 JP JP50871694A patent/JP3566287B2/ja not_active Expired - Fee Related
- 1993-10-01 HU HU9500951A patent/HU219827B/hu unknown
- 1993-10-01 SK SK420-95A patent/SK279669B6/sk not_active IP Right Cessation
- 1993-10-01 WO PCT/EP1993/002688 patent/WO1994008023A2/de active IP Right Grant
- 1993-10-01 PL PL93308301A patent/PL177635B1/pl unknown
- 1993-10-01 AU AU48202/93A patent/AU4820293A/en not_active Abandoned
- 1993-10-01 CZ CZ95809A patent/CZ285533B6/cs not_active IP Right Cessation
- 1993-10-01 AT AT93920855T patent/ATE163198T1/de active
- 1993-10-01 DK DK93920855T patent/DK0667909T3/da active
- 1993-10-01 EP EP93920855A patent/EP0667909B1/de not_active Expired - Lifetime
- 1993-10-01 US US08/411,768 patent/US6083712A/en not_active Expired - Lifetime
- 1993-10-01 EP EP97107803A patent/EP0798384A1/de not_active Withdrawn
- 1993-10-01 PL PL93333484A patent/PL179166B1/pl unknown
- 1993-10-01 ES ES93920855T patent/ES2112995T3/es not_active Expired - Lifetime
- 1993-10-01 CA CA002145400A patent/CA2145400C/en not_active Expired - Lifetime
-
1995
- 1995-03-31 FI FI951547A patent/FI117515B/fi not_active IP Right Cessation
-
2003
- 2003-09-04 JP JP2003312645A patent/JP3577075B2/ja not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| FI117515B (fi) | 2006-11-15 |
| JPH08501694A (ja) | 1996-02-27 |
| HUT71781A (en) | 1996-02-28 |
| CA2145400A1 (en) | 1994-04-14 |
| EP0667909A1 (de) | 1995-08-23 |
| PL308301A1 (en) | 1995-07-24 |
| AU4820293A (en) | 1994-04-26 |
| HU9500951D0 (en) | 1995-05-29 |
| SK279669B6 (sk) | 1999-02-11 |
| ES2112995T3 (es) | 1998-04-16 |
| EP0667909B1 (de) | 1998-02-11 |
| HU219827B (hu) | 2001-08-28 |
| KR100200542B1 (ko) | 1999-06-15 |
| JP3577075B2 (ja) | 2004-10-13 |
| JP3566287B2 (ja) | 2004-09-15 |
| CZ285533B6 (cs) | 1999-08-11 |
| DE59308149D1 (de) | 1998-03-19 |
| EP0798384A1 (de) | 1997-10-01 |
| FI951547A0 (fi) | 1995-03-31 |
| CA2145400C (en) | 2005-08-09 |
| JP2004000283A (ja) | 2004-01-08 |
| WO1994008023A3 (de) | 1994-06-23 |
| DK0667909T3 (da) | 1998-09-23 |
| PL177635B1 (pl) | 1999-12-31 |
| WO1994008023A2 (de) | 1994-04-14 |
| CZ80995A3 (en) | 1995-09-13 |
| FI951547A (fi) | 1995-03-31 |
| PL179166B1 (pl) | 2000-07-31 |
| US6083712A (en) | 2000-07-04 |
| SK42095A3 (en) | 1995-11-08 |
| ATE163198T1 (de) | 1998-02-15 |
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