KR930006159A - 유전자 조작 미생물 발효에 의한 l-페닐알라닌의 제조방법 - Google Patents

유전자 조작 미생물 발효에 의한 l-페닐알라닌의 제조방법 Download PDF

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KR930006159A
KR930006159A KR1019910015909A KR910015909A KR930006159A KR 930006159 A KR930006159 A KR 930006159A KR 1019910015909 A KR1019910015909 A KR 1019910015909A KR 910015909 A KR910015909 A KR 910015909A KR 930006159 A KR930006159 A KR 930006159A
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South Korea
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phenylalanine
promoter
producing
encoding
host cell
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KR1019910015909A
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English (en)
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KR940011838B1 (ko
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김화영
임홍
이동준
원찬희
임병락
최홍규
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하용채
주식회사 미 원
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Priority to KR1019910015909A priority Critical patent/KR940011838B1/ko
Priority to US07/851,682 priority patent/US5304475A/en
Priority to FR9209146A priority patent/FR2681330B1/fr
Priority to DE4228525A priority patent/DE4228525C2/de
Priority to ITTO920742A priority patent/IT1257392B/it
Priority to JP4241745A priority patent/JPH05244956A/ja
Priority to GB9219344A priority patent/GB2259512B/en
Publication of KR930006159A publication Critical patent/KR930006159A/ko
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Publication of KR940011838B1 publication Critical patent/KR940011838B1/ko

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/22—Tryptophan; Tyrosine; Phenylalanine; 3,4-Dihydroxyphenylalanine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/06—Alanine; Leucine; Isoleucine; Serine; Homoserine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/22—Tryptophan; Tyrosine; Phenylalanine; 3,4-Dihydroxyphenylalanine
    • C12P13/222—Phenylalanine
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/848—Escherichia
    • Y10S435/849—Escherichia coli

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Abstract

본 발명은 유전자 재조합 기술에 의해 재조합된 L-페닐알라닌의 생선성이 향상된 새로운 플라스미드에 의해 형질 전환된 숙주 세포를 배양 요지에서 배양하여 L-페닐알라닌을 생산, 축적시키고, 배양액중으로부터 L-페닐알라닌을 회수함을 특징으로 하는 L-페닐알라닌을 고수율로 제조하는 방법에 관한 것이다.

Description

유전자 조작 미생물 발효에 의한 L-페닐알라닌의 제조방법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 에쉬리시아 콜리(Escherichia coli)에 있어서 방향족 아미노산의 생합성 경로 및 조절 기작을 나타낸 것이며,
제2도는 본 발명의 재조합 플라스미드 pMW16의 제조 공정도 및 제한효소 부위도를 나타낸 것이다.

Claims (6)

  1. 코리스메이트 뮤타아제 p-프리페네이트 디히드라타제를 코딩하는 pheA 유전자, β-데옥시-D-아라비노헵툴로소네이트-7-포스페이트 신타아제를 코딩하는 AroF, 이들 두 유전자의 발현을 위한 두개의 프로모터 및 온도 감수성 리프레서 유전자를 함유하는 플라스미드에 의해 형질전환된 숙주 세포를 배지에서 배양하여 L-페닐알라닌을 생산 축적하고, 배양액으로부터 L-페닐알라닌을 회수함을 특징으로 하는 L-페닐알라닌의 제조방법.
  2. 제1항에 형질전환된 숙주 세포는 에쉬리시아 콜리(Escherichia coli)MWPWJ304(KFCC 10738)임을 특징으로 하는 제조방법.
  3. 제1항에 있어서, 프로모터는 PL프로모터임을 특징으로 하는 제조방법.
  4. 제1항에 있어서, 온도 감수성 리프레서는 C1867리프레서임을 특징으로 하는 방법.
  5. 코리스메이트 뮤타아제 p-프리페네이트 디히드라타제를 코딩하는 pheA 유전자, α-데옥시-D-아라비노헵룰로소네이트-7-포스페이트 신타아제를 코딩하는 AroF, 이들 두 유전자의 발현을 위한 두개의 프로모터 및 온도 감수성 리프레서 유전자를 함유함을 특징으로 하는 플라스미드 pMW16.
  6. 플라스미드 pMW16에 의해 형질전환된 것임을 특징으로 하는 숙주 세포.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019910015909A 1991-09-12 1991-09-12 유전자 조작 미생물 발효에 의한 l-페닐알라닌의 제조방법 KR940011838B1 (ko)

Priority Applications (7)

Application Number Priority Date Filing Date Title
KR1019910015909A KR940011838B1 (ko) 1991-09-12 1991-09-12 유전자 조작 미생물 발효에 의한 l-페닐알라닌의 제조방법
US07/851,682 US5304475A (en) 1991-09-12 1992-03-12 Method for production of L-phenylalanine by recombinant E. coli
FR9209146A FR2681330B1 (fr) 1991-09-12 1992-07-24 Nouveau plasmide recombinant replicable pour la production de phenylalanine.
DE4228525A DE4228525C2 (de) 1991-09-12 1992-08-27 Verfahren zur Herstellung von L-Phenylalanin durch rekombinante E.coli
ITTO920742A IT1257392B (it) 1991-09-12 1992-09-04 Un metodo per la produzione di l-fenilalanina mediante e.coli ricombinante
JP4241745A JPH05244956A (ja) 1991-09-12 1992-09-10 組換大腸菌によるｌ−フェニルアラニンの製造方法
GB9219344A GB2259512B (en) 1991-09-12 1992-09-10 Plasmid for the production of phenylalanine

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1019910015909A KR940011838B1 (ko) 1991-09-12 1991-09-12 유전자 조작 미생물 발효에 의한 l-페닐알라닌의 제조방법

Publications (2)

Publication Number Publication Date
KR930006159A true KR930006159A (ko) 1993-04-20
KR940011838B1 KR940011838B1 (ko) 1994-12-26

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KR1019910015909A KR940011838B1 (ko) 1991-09-12 1991-09-12 유전자 조작 미생물 발효에 의한 l-페닐알라닌의 제조방법

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US (1) US5304475A (ko)
JP (1) JPH05244956A (ko)
KR (1) KR940011838B1 (ko)
DE (1) DE4228525C2 (ko)
FR (1) FR2681330B1 (ko)
GB (1) GB2259512B (ko)
IT (1) IT1257392B (ko)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5776736A (en) * 1992-12-21 1998-07-07 Purdue Research Foundation Deblocking the common pathway of aromatic amino acid synthesis
RU2229514C2 (ru) * 2000-11-13 2004-05-27 Закрытое акционерное общество "Научно-исследовательский институт "Аджиномото-Генетика" 3-дезокси-d-арабиногептулозонат-7-фосфатсинтаза и фрагмент днк, кодирующий 3-дезокси-d-арабиногептулозонат-7-фосфатсинтазу из methylophilus methylotrophus
EP2285948B1 (en) * 2008-03-03 2014-01-08 Joule Unlimited Technologies, Inc. Engineered co2 fixing microorganisms producing carbon-based products of interest
US8048654B2 (en) 2010-06-09 2011-11-01 Joule Unlimited Technologies, Inc. Methods and compositions for the recombinant biosynthesis of fatty acids and esters
CN103725717A (zh) 2008-10-17 2014-04-16 焦耳无限科技公司 微生物的乙醇生产
WO2011011464A2 (en) * 2009-07-20 2011-01-27 Joule Unlimited, Inc. Constructs and methods for efficient transformation of micro-organisms for production of carbon-based products of interest
CN103074292B (zh) * 2013-01-22 2014-07-09 江南大学 一种高产l-苯丙氨酸的重组谷氨酸棒杆菌及其应用

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS60160890A (ja) * 1984-01-30 1985-08-22 Kyowa Hakko Kogyo Co Ltd Ｌ−フエニルアラニンの製造法
JPS61247392A (ja) * 1985-02-04 1986-11-04 エンジニツクス　インコ−ポレ−テツド ２種またはより多くのアミノ酸を同時に産生できる組換え微生物
KR890003680A (ko) * 1986-10-16 1989-04-17 죤 알.페넬 2-(2-치환 벤조일)-4-(치환)-1,3-시클로헥산디온 및 그 제조방법과 이를 이용한 조성물 및 식물체의 억제방법
US4783213A (en) * 1986-10-16 1988-11-08 Stauffer Chemical Company Certain 2-(2-substituted benzoyl)-4-(substituted oxy or substituted thio)-1,3-cyclohexanediones
KR890003682B1 (ko) * 1987-03-26 1989-09-30 주식회사 미원 유전자조작 미생물에 의한 l-페닐알라닌의 제조방법
KR890003714B1 (ko) * 1987-03-26 1989-09-30 주식회사 미원 유전자 조작 미생물에 의한 l-페닐알라닌 제조방법
US5026730A (en) * 1987-08-21 1991-06-25 Ciba-Geigy Corporation Anilinophenylthioureas, compositions containing them, and the use thereof in pest control
IL87542A (en) * 1987-08-28 1993-01-31 Uniroyal Chem Co Inc Arylenediamino substituted triazine derivatives, processes for the preparation thereof and compositions containing the same

Also Published As

Publication number Publication date
JPH05244956A (ja) 1993-09-24
GB2259512A (en) 1993-03-17
US5304475A (en) 1994-04-19
FR2681330B1 (fr) 1996-01-12
KR940011838B1 (ko) 1994-12-26
FR2681330A1 (fr) 1993-03-19
ITTO920742A1 (it) 1994-03-04
GB2259512B (en) 1995-06-21
ITTO920742A0 (it) 1992-09-04
GB9219344D0 (en) 1992-10-28
DE4228525C2 (de) 1994-11-03
IT1257392B (it) 1996-01-15
DE4228525A1 (de) 1993-06-09

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