KR880011333A - 유전자 조작 미생물에 의한 l-페닐 알라닌의 제조방법 - Google Patents

유전자 조작 미생물에 의한 l-페닐 알라닌의 제조방법 Download PDF

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Publication number
KR880011333A
KR880011333A KR870002815A KR870002815A KR880011333A KR 880011333 A KR880011333 A KR 880011333A KR 870002815 A KR870002815 A KR 870002815A KR 870002815 A KR870002815 A KR 870002815A KR 880011333 A KR880011333 A KR 880011333A
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South Korea
Prior art keywords
phenylalanine
producing
genetically engineered
engineered microorganism
phea
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KR870002815A
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English (en)
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KR890003682B1 (ko
Inventor
이새배
원찬희
박청
임번삼
Original Assignee
홍연석
주식회사 미원
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Application filed by 홍연석, 주식회사 미원 filed Critical 홍연석
Priority to KR1019870002815A priority Critical patent/KR890003682B1/ko
Priority to US07/120,148 priority patent/US5030567A/en
Publication of KR880011333A publication Critical patent/KR880011333A/ko
Application granted granted Critical
Publication of KR890003682B1 publication Critical patent/KR890003682B1/ko

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/22—Tryptophan; Tyrosine; Phenylalanine; 3,4-Dihydroxyphenylalanine
    • C12P13/222—Phenylalanine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/22—Tryptophan; Tyrosine; Phenylalanine; 3,4-Dihydroxyphenylalanine
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/848—Escherichia
    • Y10S435/849—Escherichia coli

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  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

내용없음

Description

유전자 조작 미생물에 의한 L-페닐 알라닌의 제조방법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 에쉐리시아콜리에 있어서 방향족 아미노산의 생합성 경로 및 조절기전을 나타낸 것이며,
제2도는 본발명의 재조합 유전자 PMW12 제조공정도 및 제한효소 부위도를 나타낸 것이다.

Claims (3)

  1. 신균주 MWPEC 12-45(KAIST, KCTC 8236P)를 발효배지중에 발효시켜서 L-페닐 알라닌을 제조하는 방법.
  2. 제1항에 있어서, 항생제 가나마이신 표식 유전자를 갖고 있는 PMK20 플라스미드와 PheA, aroF 유전자를 재조합 사용하는 방법.
  3. 제1항에 있어서, L-페닐 알라닌 생산에 관여하는 두 유전자(PheA, aroF)를 이용한 재조합 균주조작에 제한 효소 PstI, EcoRI를 사용하는 방법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019870002815A 1987-03-26 1987-03-26 유전자조작 미생물에 의한 l-페닐알라닌의 제조방법 KR890003682B1 (ko)

Priority Applications (2)

Application Number Priority Date Filing Date Title
KR1019870002815A KR890003682B1 (ko) 1987-03-26 1987-03-26 유전자조작 미생물에 의한 l-페닐알라닌의 제조방법
US07/120,148 US5030567A (en) 1987-03-26 1987-11-13 Method for production of L-phenylalanine by recombinant E. coli ATCC 67460

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1019870002815A KR890003682B1 (ko) 1987-03-26 1987-03-26 유전자조작 미생물에 의한 l-페닐알라닌의 제조방법

Publications (2)

Publication Number Publication Date
KR880011333A true KR880011333A (ko) 1988-10-27
KR890003682B1 KR890003682B1 (ko) 1989-09-30

Family

ID=19260314

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1019870002815A KR890003682B1 (ko) 1987-03-26 1987-03-26 유전자조작 미생물에 의한 l-페닐알라닌의 제조방법

Country Status (2)

Country Link
US (1) US5030567A (ko)
KR (1) KR890003682B1 (ko)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR940011838B1 (ko) * 1991-09-12 1994-12-26 주식회사 미원 유전자 조작 미생물 발효에 의한 l-페닐알라닌의 제조방법
US6210937B1 (en) 1997-04-22 2001-04-03 Bechtel Bwxt Idaho, Llc Development of genetically engineered bacteria for production of selected aromatic compounds
CN111187794B (zh) * 2020-04-03 2023-06-16 杭州巴洛特生物科技有限公司 一种利用大肠杆菌发酵制备l-苯丙氨酸的方法

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3128411A1 (de) * 1980-07-18 1983-09-15 Unisearch Ltd., 2033 Kensington, New South Wales Kulturstamm-mutante von esccherichia coli sowie verfahren zur herstellung von phenylalanin

Also Published As

Publication number Publication date
US5030567A (en) 1991-07-09
KR890003682B1 (ko) 1989-09-30

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