KR880011334A - 유전자 조작 미생물에 의한 l-페닐 알라닌 제조방법 - Google Patents

유전자 조작 미생물에 의한 l-페닐 알라닌 제조방법 Download PDF

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KR880011334A
KR880011334A KR870002816A KR870002816A KR880011334A KR 880011334 A KR880011334 A KR 880011334A KR 870002816 A KR870002816 A KR 870002816A KR 870002816 A KR870002816 A KR 870002816A KR 880011334 A KR880011334 A KR 880011334A
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South Korea
Prior art keywords
phenylalanine
producing
genetically engineered
phea
arof
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KR870002816A
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KR890003714B1 (ko
Inventor
이새배
원찬희
박청
임번삼
Original Assignee
홍연석
주식회사 미원
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Priority to KR1019870002816A priority Critical patent/KR890003714B1/ko
Priority to US07/120,461 priority patent/US5008190A/en
Priority to DE19883889143 priority patent/DE3889143T2/de
Priority to EP88301095A priority patent/EP0284185B1/en
Priority to JP63069928A priority patent/JPH062052B2/ja
Publication of KR880011334A publication Critical patent/KR880011334A/ko
Application granted granted Critical
Publication of KR890003714B1 publication Critical patent/KR890003714B1/ko

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C12N15/73—Expression systems using phage (lambda) regulatory sequences
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/22—Processes using, or culture media containing, cellulose or hydrolysates thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/22—Tryptophan; Tyrosine; Phenylalanine; 3,4-Dihydroxyphenylalanine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/22—Tryptophan; Tyrosine; Phenylalanine; 3,4-Dihydroxyphenylalanine
    • C12P13/222—Phenylalanine
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/848—Escherichia
    • Y10S435/849—Escherichia coli

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
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  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Virology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

내용없음

Description

유전자 조작 미생물에 의한 L-페닐 알라닌의 제조방법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 에쉐레시아 콜리에 있어서 방향족 아미노산의 생합성 경로 및 조절기전을 나타낸 것이며,
제2도는 본발명의 재조합 유전자 PMW12 제조공정도 및 제한효소 부위도를 나타낸 것이며,
제3도는 본발명의 재조합 유전자 PMW13 제조공정도 및 제한효소 부위도를 나타낸 것이다.
내용없음

Claims (4)

  1. 신균주 MWPEC 13-60(KAIST, KCTC 8237P)를 발효배지중에 발효시켜서 L-페닐 알라닌을 제조하는 방법.
  2. 제1항에 있어서, 항생제 가나마이신 표식 유전자를 갖고 있는 PMK20 플라스미드와 본문의 상세한 설명에서 사용한 PheA 및 aroF 유전자를 재조합하여 사용하는 방법.
  3. 제2항에서, L-페닐 알라닌 생합성 관여 유전자(PheA, aroF)를 이용한 재조합 플라스미드조작에 제한 효소 PstI, EcoRI, HindⅢ를 사용하는 방법.
  4. 제3항에서 벙이된 온도 조절유전자를 이용 재조합 플라스미드조작에 제한효소 AatI, HacII, BamHI, BglII, PstI를 사용하는 방법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019870002816A 1987-03-26 1987-03-26 유전자 조작 미생물에 의한 l-페닐알라닌 제조방법 KR890003714B1 (ko)

Priority Applications (5)

Application Number Priority Date Filing Date Title
KR1019870002816A KR890003714B1 (ko) 1987-03-26 1987-03-26 유전자 조작 미생물에 의한 l-페닐알라닌 제조방법
US07/120,461 US5008190A (en) 1987-03-26 1987-11-13 Method for production of L-phenylalanine by recombinant E. coli
DE19883889143 DE3889143T2 (de) 1987-03-26 1988-02-10 Verfahren zur Herstellung von L-Phenylalanin durch rekombinantes E.coli.
EP88301095A EP0284185B1 (en) 1987-03-26 1988-02-10 A method for production of L-phenylalanine by recombinant E. coli
JP63069928A JPH062052B2 (ja) 1987-03-26 1988-03-25 遺伝子組換え大腸菌及びそれを用いるｌ‐フエニルアラニンの製造方法

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1019870002816A KR890003714B1 (ko) 1987-03-26 1987-03-26 유전자 조작 미생물에 의한 l-페닐알라닌 제조방법

Publications (2)

Publication Number Publication Date
KR880011334A true KR880011334A (ko) 1988-10-27
KR890003714B1 KR890003714B1 (ko) 1989-09-30

Family

ID=19260316

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1019870002816A KR890003714B1 (ko) 1987-03-26 1987-03-26 유전자 조작 미생물에 의한 l-페닐알라닌 제조방법

Country Status (4)

Country Link
US (1) US5008190A (ko)
EP (1) EP0284185B1 (ko)
JP (1) JPH062052B2 (ko)
KR (1) KR890003714B1 (ko)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0623605B2 (ja) * 1987-05-26 1994-03-30 日本ファーネス工業株式会社 ラジアントチューブバーナ
KR940011838B1 (ko) * 1991-09-12 1994-12-26 주식회사 미원 유전자 조작 미생물 발효에 의한 l-페닐알라닌의 제조방법
US6210937B1 (en) 1997-04-22 2001-04-03 Bechtel Bwxt Idaho, Llc Development of genetically engineered bacteria for production of selected aromatic compounds
EP3312284A3 (en) 2010-07-26 2018-05-30 Genomatica, Inc. Microorganisms and methods for the biosynthesis of aromatics, 2,4-pentadienoate and 1,3-butadiene

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3128411A1 (de) * 1980-07-18 1983-09-15 Unisearch Ltd., 2033 Kensington, New South Wales Kulturstamm-mutante von esccherichia coli sowie verfahren zur herstellung von phenylalanin
AU3187984A (en) * 1983-08-16 1985-02-21 Genentech Inc. Recombinant process for preparing l-amino acids
US4839286A (en) * 1983-10-07 1989-06-13 Biotechnica International, Inc. Method of biosynthesis and cells therefor
GB8333797D0 (en) * 1983-12-19 1984-01-25 Searle & Co Starch utilization
JPS6192565A (ja) * 1984-09-24 1986-05-10 バイオテクニカ・インターナシヨナル・インコーポレーテツド 生合成方法およびそのための細胞
CA1283375C (en) * 1984-09-24 1991-04-23 H.J. Heinz Company Method of biosynthesis and cells therefor
EP0190921A3 (en) * 1985-02-04 1988-01-13 Engenics, Inc. Method for the overproduction of amino acids
JPS63500215A (ja) * 1985-06-24 1988-01-28 ザ　ヌトラスウイ−ト　カンパニ− アミノ酸合成用の複合プラスミド

Also Published As

Publication number Publication date
EP0284185B1 (en) 1994-04-20
KR890003714B1 (ko) 1989-09-30
US5008190A (en) 1991-04-16
JPH01104160A (ja) 1989-04-21
JPH062052B2 (ja) 1994-01-12
EP0284185A1 (en) 1988-09-28

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