KR950700329A - 신규한 세포 표층 단백질(Novel cell cortex protein) - Google Patents

신규한 세포 표층 단백질(Novel cell cortex protein)

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Publication number
KR950700329A
KR950700329A KR1019940703193A KR19940703193A KR950700329A KR 950700329 A KR950700329 A KR 950700329A KR 1019940703193 A KR1019940703193 A KR 1019940703193A KR 19940703193 A KR19940703193 A KR 19940703193A KR 950700329 A KR950700329 A KR 950700329A
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South Korea
Prior art keywords
leu
ala
cell surface
asp
surface protein
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KR1019940703193A
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English (en)
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KR0164235B1 (ko
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히사시 가와사키
마코토 쓰치야
기요시 미와
요시오 가와하라
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도바 다다스
아지노모토 가부시키가이샤
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/345—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Brevibacterium (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/77—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Corynebacterium; for Brevibacterium

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  • Chemical & Material Sciences (AREA)
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  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
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  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
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  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
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  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)

Abstract

분자내 2개의 서열
(1) Thr-Leu-Arg-Gln-His-Tyr-Ser-Ser-Leu-Ile-Pro-Asn-Leu-Phe-Ile-Ala-Ala-Val-Gly-Asn-Ile-Asn-Glu-Leu-Asn-Asn-Ala-Asp-Gln-Ala-Ala-Arg-Glu-Leu-Phe-Leu-Asp-Trp-Asp-Thr, 및
(2) Asn-Lys-Thr-Asp-Phe-Ala-Glu-Ile-Glu-Leu-Tyr-Asp-Val-Leu-Tyr-Thr-Asp-Ala-Asp-Ile-Ser-Gly-Asp-Ala-Pro-Leu-Leu-Ala-Pro-Ala-Tyr-Lys.
을 갖고, 분자량이 약 63,000달톤인 브레비박테리움 락토페르멘툼(Brevibacterium lactofermentum)으로부터 유도되는 신규한 세포 표증 단백질의 암호화 유전자를 포함하는 DNA 단편을 코리네포름 세균에 도입시켜 형질전환체를 제조한다. 새로이 제조된, 신규한 세포 표층 단백질이 부족한 이러한 형질전환체 또는 균주는 L-아미노산 발효법에 의해 L-아민노산을 포함한 유용물질을 제조하는데 사용된다.

Description

신규한 세포 표층 단백질(Novel cell cortex protein)
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도 : 상동 재조합에 의해 유전자를 붕괴시키기 위한 도식도, 제2도 : 암모늄 이온의 삽입을 위한 K-단백질의 기여를 나타냄.

Claims (11)

  1. 분자내 2개의 서열
    (1) Thr-Leu-Arg-Gln-His-Tyr-Ser-Ser-Leu-Ile-Pro-Asn-Leu-Phe-Ile-Ala-Ala-Val-Gly-Asn-Ile-Asn-Glu-Leu-Asn-Asn-Ala-Asp-Gln-Ala-Ala-Arg-Glu-Leu-Phe-Leu-Asp-Trp-Asp-Thr, 및
    (2) Asn-Lys-Thr-Asp-Phe-Ala-Glu-Ile-Glu-Leu-Tyr-Asp-Val-Leu-Tyr-Thr-Asp-Ala-Asp-Ile-Ser-Gly-Asp-Ala-Pro-Leu-Leu-Ala-Pro-Ala-Tyr-Lys.
    을 갖고, 분자량이 약 63,000달톤인 브레비박테리움 락토페르멘툼(Brevibacterium lactofermentum)으로부터 유도되는 세포 표층 단백질.
  2. 제1항에 따른 세포 표층 단백질의 암호화 유전자를 포함하는 DNA 단편.
  3. 제2항에 다른 DNA 단편을 코리네포름(Coryneform) 세균 세포에서 자동 복제할 수 있는 벡터와 결합시켜 수득된 재조합 DNA.
  4. 제2항에 따른 DNA 단편을 코리네포름 세균 세포에서 증폭시켜 수득된 형질전환체.
  5. 유용 물질 생성 활성을 갖는 제4항에 다른 형질전환체를 배양 배지에서 배양하고, 배양 배지에 유용물질을 생성 및 축척시킨 후, 배양 배지로부터 유용물질을 수집하는 단계를 포함하여 발효 과정에 의해 유용물질을 제조하는 방법.
  6. 제5항에 있어서, 유용물질이 L-아미노산인 방법.
  7. 제1항의 세포 표층 단백질 또는 이 세포 표층 단백질과 실질적으로 동일한 단백질이 부족하고 세포 응집 특성이 있음을 특징으로 하는 코리네포름 세균.
  8. 제7항에 있어서, 제1항에 따른 세포 표층 단백질의 암호화 유전자 또는 이 세포 표층 단백질과 실질적으로 동일하고 코리네포름 세균의 세포 표층에 존재하는 단백질의 암호화 유전자가, 세포 표층 단백질의 암호화 유전자 일부분 이상을 포함하는 DNA 단편과 이 DNA 단편과 동일하거나 상동성인 염색체상의 DNA 서열과의 상동 재조합(homologous recombination)에 의해 파괴됨을 특징으로 하는 코리네포름 세균.
  9. 제7항 또는 제8항에 있어서, 코리네포름 세균이 브레비박테리움 락토페르멘툼인 코리네포름 세균.
  10. 유용물질을 생성하는 활성을 갖는, 제7항 내지 제9항중 어느 한 항에 따른 코리네포름 세균을 배양하고, 유용물질을 배양 배지에 생성 및 축적시킨후, 유용물질을 배양 배지로부터 수집하며, 추가로 배양 완료후 배양 배지를 정치시켜 코리네포름 세균의 세포를 침전시키는 단계를 포함하여 유용물질을 제조하는 방법.
  11. 제10항에 있어서, 유용물질이 L-아미노산인 방법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019940703193A 1993-01-13 1994-01-13 신규한 세포 표층 단백질 KR0164235B1 (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP93-4069 1993-01-13
JP406993 1993-01-13
PCT/JP1994/000039 WO1994015963A1 (en) 1993-01-13 1994-01-13 Novel cell cortex protein

Publications (2)

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KR950700329A true KR950700329A (ko) 1995-01-16
KR0164235B1 KR0164235B1 (ko) 1999-01-15

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US (2) US5547864A (ko)
EP (1) EP0644200B1 (ko)
JP (1) JP3541385B2 (ko)
KR (1) KR0164235B1 (ko)
CN (2) CN1036850C (ko)
AT (1) ATE165606T1 (ko)
BR (1) BR9403528A (ko)
DE (1) DE69409895T2 (ko)
ES (1) ES2115208T3 (ko)
WO (1) WO1994015963A1 (ko)

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DE19903345A1 (de) * 1999-01-28 2000-08-03 Werner Lubitz Kompartimentierung von rekombinanten Polypeptiden in Wirtszellen
US6927046B1 (en) * 1999-12-30 2005-08-09 Archer-Daniels-Midland Company Increased lysine production by gene amplification using coryneform bacteria
DE60234247D1 (de) * 2001-03-30 2009-12-17 Ajinomoto Kk Verfahren zur sekretion und produktion von protein
BR0317132A (pt) * 2002-12-09 2005-10-25 Chidren S Hospital Medical Ct Métodos de diagnóstico e tratamento de doença pulmonar intersticial
WO2005010144A2 (en) * 2003-04-21 2005-02-03 Wisconsin Alumni Research Foundation Displacing a plasmid in a bacterial population
WO2014126260A1 (ja) * 2013-02-18 2014-08-21 味の素株式会社 タンパク質の沈殿による製造方法

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JP3541385B2 (ja) 2004-07-07
ATE165606T1 (de) 1998-05-15
DE69409895T2 (de) 1998-12-24
KR0164235B1 (ko) 1999-01-15
CN1101208A (zh) 1995-04-05
US5547864A (en) 1996-08-20
CN1124339C (zh) 2003-10-15
ES2115208T3 (es) 1998-06-16
US5681717A (en) 1997-10-28
DE69409895D1 (de) 1998-06-04
BR9403528A (pt) 1999-05-25
EP0644200B1 (en) 1998-04-29
EP0644200A1 (en) 1995-03-22
CN1036850C (zh) 1997-12-31
EP0644200A4 (en) 1996-01-24
WO1994015963A1 (en) 1994-07-21
CN1160081A (zh) 1997-09-24

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