IT1257392B - Un metodo per la produzione di l-fenilalanina mediante e.coli ricombinante - Google Patents

Un metodo per la produzione di l-fenilalanina mediante e.coli ricombinante

Info

Publication number
IT1257392B
IT1257392B ITTO920742A ITTO920742A IT1257392B IT 1257392 B IT1257392 B IT 1257392B IT TO920742 A ITTO920742 A IT TO920742A IT TO920742 A ITTO920742 A IT TO920742A IT 1257392 B IT1257392 B IT 1257392B
Authority
IT
Italy
Prior art keywords
coli
gene
recombinant
phenylalanin
production
Prior art date
Application number
ITTO920742A
Other languages
English (en)
Inventor
Hwa Young Kim
Hong Rhym
Dond Joon Lee
Chan Hee Won
Byung Lak Lim
Hong Kyu Choi
Original Assignee
Miwon Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Miwon Co Ltd filed Critical Miwon Co Ltd
Publication of ITTO920742A0 publication Critical patent/ITTO920742A0/it
Publication of ITTO920742A1 publication Critical patent/ITTO920742A1/it
Application granted granted Critical
Publication of IT1257392B publication Critical patent/IT1257392B/it

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/22—Tryptophan; Tyrosine; Phenylalanine; 3,4-Dihydroxyphenylalanine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/06—Alanine; Leucine; Isoleucine; Serine; Homoserine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/22—Tryptophan; Tyrosine; Phenylalanine; 3,4-Dihydroxyphenylalanine
    • C12P13/222—Phenylalanine
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/848—Escherichia
    • Y10S435/849—Escherichia coli

Landscapes

  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Un nuovo E.coli (RCCM 10.031) che può produrre rese elevate di L-fenilalanina e un metodo la produzione di L-fenilalanina mediante E.coli ricombinante trasformabile con un nuovo plasmidio pMW16 contenente due promotori e un repressore sensibile alla temperatura per manifestare un gene pheA e un gene aroF in cui corismato mutasi p-prefenato deidratasi sia codificata dal gene pheA e 3-deossi-D-arabinoeptulosonato 7-fosfato sintasi sia codificato dal gene aroF.
ITTO920742A 1991-09-12 1992-09-04 Un metodo per la produzione di l-fenilalanina mediante e.coli ricombinante IT1257392B (it)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1019910015909A KR940011838B1 (ko) 1991-09-12 1991-09-12 유전자 조작 미생물 발효에 의한 l-페닐알라닌의 제조방법

Publications (3)

Publication Number Publication Date
ITTO920742A0 ITTO920742A0 (it) 1992-09-04
ITTO920742A1 ITTO920742A1 (it) 1994-03-04
IT1257392B true IT1257392B (it) 1996-01-15

Family

ID=19319858

Family Applications (1)

Application Number Title Priority Date Filing Date
ITTO920742A IT1257392B (it) 1991-09-12 1992-09-04 Un metodo per la produzione di l-fenilalanina mediante e.coli ricombinante

Country Status (7)

Country Link
US (1) US5304475A (it)
JP (1) JPH05244956A (it)
KR (1) KR940011838B1 (it)
DE (1) DE4228525C2 (it)
FR (1) FR2681330B1 (it)
GB (1) GB2259512B (it)
IT (1) IT1257392B (it)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5776736A (en) * 1992-12-21 1998-07-07 Purdue Research Foundation Deblocking the common pathway of aromatic amino acid synthesis
RU2229514C2 (ru) * 2000-11-13 2004-05-27 Закрытое акционерное общество "Научно-исследовательский институт "Аджиномото-Генетика" 3-дезокси-d-арабиногептулозонат-7-фосфатсинтаза и фрагмент днк, кодирующий 3-дезокси-d-арабиногептулозонат-7-фосфатсинтазу из methylophilus methylotrophus
US8048654B2 (en) 2010-06-09 2011-11-01 Joule Unlimited Technologies, Inc. Methods and compositions for the recombinant biosynthesis of fatty acids and esters
EP2706111A1 (en) * 2008-03-03 2014-03-12 Joule Unlimited Technologies, Inc. Engineered CO2 fixing microorganisms producing carbon-based products of interest
EP2998402A1 (en) 2008-10-17 2016-03-23 Joule Unlimited Technologies, Inc. Ethanol production by microorganisms
WO2011011464A2 (en) * 2009-07-20 2011-01-27 Joule Unlimited, Inc. Constructs and methods for efficient transformation of micro-organisms for production of carbon-based products of interest
CN103074292B (zh) * 2013-01-22 2014-07-09 江南大学 一种高产l-苯丙氨酸的重组谷氨酸棒杆菌及其应用

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS60160890A (ja) * 1984-01-30 1985-08-22 Kyowa Hakko Kogyo Co Ltd Ｌ−フエニルアラニンの製造法
JPS61247392A (ja) * 1985-02-04 1986-11-04 エンジニツクス　インコ−ポレ−テツド ２種またはより多くのアミノ酸を同時に産生できる組換え微生物
US4783213A (en) * 1986-10-16 1988-11-08 Stauffer Chemical Company Certain 2-(2-substituted benzoyl)-4-(substituted oxy or substituted thio)-1,3-cyclohexanediones
KR890003680A (ko) * 1986-10-16 1989-04-17 죤 알.페넬 2-(2-치환 벤조일)-4-(치환)-1,3-시클로헥산디온 및 그 제조방법과 이를 이용한 조성물 및 식물체의 억제방법
KR890003714B1 (ko) * 1987-03-26 1989-09-30 주식회사 미원 유전자 조작 미생물에 의한 l-페닐알라닌 제조방법
KR890003682B1 (ko) * 1987-03-26 1989-09-30 주식회사 미원 유전자조작 미생물에 의한 l-페닐알라닌의 제조방법
US5026730A (en) * 1987-08-21 1991-06-25 Ciba-Geigy Corporation Anilinophenylthioureas, compositions containing them, and the use thereof in pest control
IL87542A (en) * 1987-08-28 1993-01-31 Uniroyal Chem Co Inc Arylenediamino substituted triazine derivatives, processes for the preparation thereof and compositions containing the same

Also Published As

Publication number Publication date
FR2681330B1 (fr) 1996-01-12
FR2681330A1 (fr) 1993-03-19
GB2259512A (en) 1993-03-17
KR940011838B1 (ko) 1994-12-26
KR930006159A (ko) 1993-04-20
DE4228525C2 (de) 1994-11-03
US5304475A (en) 1994-04-19
ITTO920742A0 (it) 1992-09-04
GB9219344D0 (en) 1992-10-28
ITTO920742A1 (it) 1994-03-04
DE4228525A1 (de) 1993-06-09
JPH05244956A (ja) 1993-09-24
GB2259512B (en) 1995-06-21

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Legal Events

Date Code Title Description
0001 Granted
TA Fee payment date (situation as of event date), data collected since 19931001

Effective date: 19970924