KR880012760A - 이작용성 단백질 - Google Patents

이작용성 단백질 Download PDF

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Publication number
KR880012760A
KR880012760A KR1019880004345A KR880004345A KR880012760A KR 880012760 A KR880012760 A KR 880012760A KR 1019880004345 A KR1019880004345 A KR 1019880004345A KR 880004345 A KR880004345 A KR 880004345A KR 880012760 A KR880012760 A KR 880012760A
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KR
South Korea
Prior art keywords
protein
asp
thr
pro
component
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KR1019880004345A
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English (en)
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KR970000187B1 (ko
Inventor
하버만 파울
Original Assignee
하인리히 벡커, 베른하르트 벡
훽스트 아크티엔게젤샤프트
베른하르트 벡
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Application filed by 하인리히 벡커, 베른하르트 벡, 훽스트 아크티엔게젤샤프트, 베른하르트 벡 filed Critical 하인리히 벡커, 베른하르트 벡
Publication of KR880012760A publication Critical patent/KR880012760A/ko
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Publication of KR970000187B1 publication Critical patent/KR970000187B1/ko

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/53—Colony-stimulating factor [CSF]
    • C07K14/535—Granulocyte CSF; Granulocyte-macrophage CSF
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/54—Interleukins [IL]
    • C07K14/55—IL-2
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones

Abstract

내용 없음

Description

이작용성 단백질
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
도면은 본 발명에 따른 이작용성 단백질을 암호화하는 플라스미드 PB30의 작제를 나타낸다.

Claims (7)

  1. 생물학적으로 활성인 인터로이킨-2(IL-2) 성분 및 과립구 대식세포 콜로니 자극 인자(GM-CSF)성분으로 구성된 이작용성 단백질.
  2. 제 1 항에 있어서, 두개의 생물학적으로 활성인 단백질 성분이 유전적으로 암호화가능한 1 내지 약 20 개의 아미노산으로 구성된 가교에 의해 연결되는 단백질.
  3. 제 2 항에 있어서, 가교가 하기 일반식(II)에 상응하는 단밸질.
    -Asp-(aa)x)-Pro-
    상기식에서 x는 1 내지 18의 정수이고 aa는 cys를 제외하고는 유전적으로 암호화가능한 아미노산이다.
  4. 제 3 항에 있어서, 가교멤버(aa)x가 아미노산서열-Asp-Pro-Met-Ile-Thr-Thr-Tyr-Ala-Asp-Asp-Pro-또는-Pro-Met-Ile-Thr-Thr-Tyr-Leu-Glu-Glu-Leu-Thr-Ile-Asp-Asp-을 나타내는 단백질.
  5. 제 1 항 내지 제 4 항중 어느 한 항에 있어서, IL-2성분이 N-말단에 배열되고 GM-CSF 성분이 C-말단에 배열되는 단백질.
  6. 제 1 항 내지 5 항중 어느 한 항에서 청구된 이작용성 단백질을 암호화하는 유전자의 형성, 및 숙주세포내에서의 발현을 포함하는 이작용성 단백질의 제조방법.
  7. 필요에 따라 약리학적으로 적합한 비히클과 혼합되는 제 1 항 내지 5 항중 어느 한 항에서 청구된 단백질로 구성된 약제.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019880004345A 1987-04-16 1988-04-16 이작용성 단백질, 이의 제조방법 및 이를 포함하는 약제 KR970000187B1 (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DEP3712985.6 1987-04-16
DE19873712985 DE3712985A1 (de) 1987-04-16 1987-04-16 Bifunktionelle proteine
DEP3712985.5 1987-04-16

Publications (2)

Publication Number Publication Date
KR880012760A true KR880012760A (ko) 1988-11-29
KR970000187B1 KR970000187B1 (ko) 1997-01-06

Family

ID=6325799

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1019880004345A KR970000187B1 (ko) 1987-04-16 1988-04-16 이작용성 단백질, 이의 제조방법 및 이를 포함하는 약제

Country Status (20)

Country Link
EP (1) EP0288809B1 (ko)
JP (1) JP2667193B2 (ko)
KR (1) KR970000187B1 (ko)
AR (1) AR242991A1 (ko)
AT (1) ATE79135T1 (ko)
AU (1) AU613022B2 (ko)
CA (1) CA1322157C (ko)
DE (2) DE3712985A1 (ko)
DK (1) DK170741B1 (ko)
ES (1) ES2033981T3 (ko)
FI (1) FI98830C (ko)
GR (1) GR3006141T3 (ko)
HU (1) HU204303B (ko)
IE (1) IE61574B1 (ko)
IL (1) IL86086A (ko)
NO (1) NO176922C (ko)
NZ (1) NZ224247A (ko)
PH (1) PH25327A (ko)
PT (1) PT87237B (ko)
ZA (1) ZA882659B (ko)

Families Citing this family (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5662896A (en) * 1988-03-21 1997-09-02 Chiron Viagene, Inc. Compositions and methods for cancer immunotherapy
US5225538A (en) * 1989-02-23 1993-07-06 Genentech, Inc. Lymphocyte homing receptor/immunoglobulin fusion proteins
US5116964A (en) * 1989-02-23 1992-05-26 Genentech, Inc. Hybrid immunoglobulins
US6406697B1 (en) 1989-02-23 2002-06-18 Genentech, Inc. Hybrid immunoglobulins
AU5355790A (en) * 1989-04-19 1990-11-16 Cetus Corporation Multifunctional m-csf proteins and genes encoding therefor
US5108910A (en) * 1989-08-22 1992-04-28 Immunex Corporation DNA sequences encoding fusion proteins comprising GM-CSF and IL-3
US5073627A (en) * 1989-08-22 1991-12-17 Immunex Corporation Fusion proteins comprising GM-CSF and IL-3
ATE103932T1 (de) * 1989-08-22 1994-04-15 Immunex Corp Fusionsprotein bestehend aus gm-csf und il-3.
US5376367A (en) * 1991-11-22 1994-12-27 Immunex Corporation Fusion proteins comprising MGF and IL-3
US5723125A (en) * 1995-12-28 1998-03-03 Tanox Biosystems, Inc. Hybrid with interferon-alpha and an immunoglobulin Fc linked through a non-immunogenic peptide
WO1999029732A2 (en) 1997-12-08 1999-06-17 Lexigen Pharmaceuticals Corporation Heterodimeric fusion proteins useful for targeted immune therapy and general immune stimulation
SK782002A3 (en) 1999-07-21 2003-08-05 Lexigen Pharm Corp FC fusion proteins for enhancing the immunogenicity of protein and peptide antigens
MXPA02001417A (es) 1999-08-09 2002-08-12 Lexigen Pharm Corp Complejos multiples de citosina-anticuerpo.
AU1551801A (en) * 1999-11-30 2001-06-12 Shionogi & Co., Ltd. Chemokine slc-il2 fused protein and gene thereof
EP1252192B1 (en) 2000-02-11 2006-08-16 MERCK PATENT GmbH Enhancing the circulating half-life of antibody-based fusion proteins
DK1366067T3 (da) 2001-03-07 2012-10-22 Merck Patent Gmbh Ekspressionsteknologi for proteiner indeholdende en hybrid isotype-antistof-enhed
US6992174B2 (en) 2001-03-30 2006-01-31 Emd Lexigen Research Center Corp. Reducing the immunogenicity of fusion proteins
BR0209177A (pt) 2001-05-03 2004-10-05 Merck Patent Gmbh Anticorpo especìfico a tumor recombinante e uso do mesmo
EP2292271A3 (en) 2001-10-10 2011-09-14 BioGeneriX AG Remodelling and glycoconjugation of an antibody
PT1454138E (pt) 2001-12-04 2012-03-28 Merck Patent Gmbh Imunocitoquinas com seletividade modulada
CA2510180C (en) 2002-12-17 2012-09-11 Merck Patent Gesellschaft Mit Beschraenkter Haftung Humanized antibody (h14.18) of the mouse 14.18 antibody binding to gd2 and its fusion with il-2
ES2359473T3 (es) * 2003-07-21 2011-05-23 Transgene S.A. Citoquinas multifuncionales.
DE602004031341D1 (de) 2003-07-21 2011-03-24 Transgene Sa Multifunktionelle cytokine
WO2010001414A1 (en) * 2008-07-03 2010-01-07 Lupin Limited Expression of heterologous proteins in bacterial system using a gm-csf fusion tag

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1985000817A1 (en) * 1983-08-10 1985-02-28 Amgen Microbial expression of interleukin ii
GB8327880D0 (en) * 1983-10-18 1983-11-16 Ajinomoto Kk Saccharomyces cerevisiae
EP0158198A1 (en) * 1984-03-29 1985-10-16 Takeda Chemical Industries, Ltd. DNA and use thereof
WO1985004673A1 (en) * 1984-04-10 1985-10-24 Takeda Chemical Industries, Ltd. Novel dna and its use
DE3419995A1 (de) * 1984-05-29 1985-12-05 Hoechst Ag, 6230 Frankfurt Gentechnologisches verfahren zur herstellung von human-interleukin-2 und mittel zur durchfuehrung dieses verfahrens
AU588819B2 (en) * 1984-10-29 1989-09-28 Immunex Corporation Cloning of human granulocyte-macrophage colony stimulating factor gene
JPS61128889A (ja) * 1984-11-27 1986-06-16 Green Cross Corp:The 組換えｄｎａ及び該ｄｎａによる形質転換体
JPH0646957B2 (ja) * 1985-03-11 1994-06-22 武田薬品工業株式会社 インタ−ロイキン−２の製造方法
JPS63502795A (ja) * 1985-10-03 1988-10-20 バイオジェン インコーポレイテッド 顆粒球‐マクロファージコロニー刺激因子‐様ポリペプチド、ｄｎａ配列、組換えｄｎａ分子並びに微生物細胞中でヒト顆粒球−マクロファ−ジコロニ−刺激因子−様ポリペプチドを高収量で生産する方法
DE3541856A1 (de) * 1985-11-27 1987-06-04 Hoechst Ag Eukaryotische fusionsproteine, ihre herstellung und verwendung sowie mittel zur durchfuehrung des verfahrens
DE3545568A1 (de) * 1985-12-21 1987-07-16 Hoechst Ag Gm-csf-protein, seine derivate, herstellung solcher proteine und ihre verwendung

Also Published As

Publication number Publication date
FI98830B (fi) 1997-05-15
EP0288809A1 (de) 1988-11-02
ZA882659B (en) 1988-10-14
PT87237B (pt) 1992-07-31
KR970000187B1 (ko) 1997-01-06
EP0288809B1 (de) 1992-08-05
DE3873397D1 (de) 1992-09-10
DE3712985A1 (de) 1988-11-03
NO176922B (no) 1995-03-13
FI881743A (fi) 1988-10-17
FI881743A0 (fi) 1988-04-14
GR3006141T3 (ko) 1993-06-21
HUT47319A (en) 1989-02-28
JP2667193B2 (ja) 1997-10-27
DK170741B1 (da) 1996-01-08
NO881658D0 (no) 1988-04-15
CA1322157C (en) 1993-09-14
AU1466188A (en) 1988-10-20
IE61574B1 (en) 1994-11-16
AR242991A1 (es) 1993-06-30
DK209188A (da) 1988-10-17
NO881658L (no) 1988-10-17
PT87237A (pt) 1988-05-01
NZ224247A (en) 1990-04-26
DK209188D0 (da) 1988-04-15
NO176922C (no) 1995-06-21
HU204303B (en) 1991-12-30
ES2033981T3 (es) 1993-04-01
IL86086A (en) 1995-01-24
FI98830C (fi) 1997-08-25
IE881146L (en) 1988-10-16
PH25327A (en) 1991-04-30
AU613022B2 (en) 1991-07-25
IL86086A0 (en) 1988-09-30
JPS63301898A (ja) 1988-12-08
ATE79135T1 (de) 1992-08-15

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