KR870006188A - 과립구 대식세포 콜로니 촉진 인자(gm-csf) 단백질의 제조방법 - Google Patents

과립구 대식세포 콜로니 촉진 인자(gm-csf) 단백질의 제조방법 Download PDF

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Publication number
KR870006188A
KR870006188A KR860011011A KR860011011A KR870006188A KR 870006188 A KR870006188 A KR 870006188A KR 860011011 A KR860011011 A KR 860011011A KR 860011011 A KR860011011 A KR 860011011A KR 870006188 A KR870006188 A KR 870006188A
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KR
South Korea
Prior art keywords
csf
macrophage colony
granulocyte macrophage
protein
preparing
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KR860011011A
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English (en)
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KR940005585B1 (ko
Inventor
폴하베르만
뮐네르 후베르트
Original Assignee
하인리히 벡커, 베른하르트 벡
훽스트 아크티엔게젤샤프트
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Publication of KR870006188A publication Critical patent/KR870006188A/ko
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Publication of KR940005585B1 publication Critical patent/KR940005585B1/ko

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/54—Interleukins [IL]
    • C07K14/55—IL-2
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/53—Colony-stimulating factor [CSF]
    • C07K14/535—Granulocyte CSF; Granulocyte-macrophage CSF
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones

Abstract

내용 없음

Description

과립구 대식세포 콜로니 촉진 인자(GM-CSF) 단백질의 제조방법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도 및 그의 연속도면인 제1a와 1b도는 (Met-) CSF의 직접발현용 벡터 pW225의 제법이다. 다음의 도면들은 융합단백질을 발현시키는 벡터에 관한 것으로 이후 “IL-2”또는 “IL-2”로 언급할 사람 인터루킨-2의 부분-서열로부터 유도한 “안정(ballast)”단백질이 CSF아미노산 서열의 앞인 N-종결말단에 위치하고 있다.

Claims (4)

  1. CSF암호화 유전자를 세균성 발현 벡터에 삽입하고, 세균을 상기벡터로 형질전화시키며, 발현을 유도하여 사람 과립구 대식세포 콜로니-촉진 인자 단백질(CSF)을 제조하는 방법.
  2. 제1항에 있어서, 세균이 이. 콜라이(E. Coli)인 방법.
  3. 제1 또는 2항에 있어서, CSF를 융합단백질 형태로 발현시키고 이어서 효소적으로 또는 화학적으로 절단하는 방법.
  4. 제3항에 있어서, 융합단백질이 CSF에 인접한 N-종결부에 아미노산서열(Glu)m-(Asp)n-Pro[여기에서, m은 0또는 1이고, n은 1,2 또는 3이다]을 함유하여 이를 단백분해적으로 절단하는 방법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019860011011A 1985-12-21 1986-12-20 과립구 대식세포 콜로니 촉진 인자(gm-csf)단백질의 제조방법 KR940005585B1 (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DEP3545568.3 1985-12-21
DE19853545568 DE3545568A1 (de) 1985-12-21 1985-12-21 Gm-csf-protein, seine derivate, herstellung solcher proteine und ihre verwendung

Publications (2)

Publication Number Publication Date
KR870006188A true KR870006188A (ko) 1987-07-09
KR940005585B1 KR940005585B1 (ko) 1994-06-21

Family

ID=6289242

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1019860011011A KR940005585B1 (ko) 1985-12-21 1986-12-20 과립구 대식세포 콜로니 촉진 인자(gm-csf)단백질의 제조방법

Country Status (17)

Country Link
EP (1) EP0228018B1 (ko)
JP (1) JP2575367B2 (ko)
KR (1) KR940005585B1 (ko)
AT (1) ATE71144T1 (ko)
AU (2) AU601959B2 (ko)
CA (1) CA1341197C (ko)
DE (2) DE3545568A1 (ko)
DK (1) DK170346B1 (ko)
ES (1) ES2055686T3 (ko)
FI (1) FI91168C (ko)
GR (1) GR3003999T3 (ko)
HU (1) HU202584B (ko)
IE (1) IE59779B1 (ko)
IL (1) IL81020A (ko)
NO (1) NO177270C (ko)
PT (1) PT83972B (ko)
ZA (1) ZA869557B (ko)

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Publication number Priority date Publication date Assignee Title
KR100448021B1 (ko) * 2001-12-28 2004-09-08 크레아젠 주식회사 마우스 과립구 대식세포 콜로니 촉진인자 발현벡터 ｐＧＭ-ＣＳＦ로 형질전환된 대장균 및 상기 마우스 과립구 대식세포 콜로니 촉진인자의 대량생산방법

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US5078996A (en) * 1985-08-16 1992-01-07 Immunex Corporation Activation of macrophage tumoricidal activity by granulocyte-macrophage colony stimulating factor
JPS63502795A (ja) * 1985-10-03 1988-10-20 バイオジェン インコーポレイテッド 顆粒球‐マクロファージコロニー刺激因子‐様ポリペプチド、ｄｎａ配列、組換えｄｎａ分子並びに微生物細胞中でヒト顆粒球−マクロファ−ジコロニ−刺激因子−様ポリペプチドを高収量で生産する方法
DE3712985A1 (de) * 1987-04-16 1988-11-03 Hoechst Ag Bifunktionelle proteine
US5359035A (en) * 1985-12-21 1994-10-25 Hoechst Aktiengesellschaft Bifunctional proteins including interleukin-2 (IL-2) and granuloctyte macrophage colony stimulating factor (GM-CSF)
JP2583770B2 (ja) * 1986-09-17 1997-02-19 大塚製薬株式会社 遺伝子
JPS6420097A (en) * 1987-03-02 1989-01-24 Sumitomo Chemical Co Human granulocyte-macrophage colony stimulating factor
PT87133B (pt) * 1987-04-02 1992-07-31 Amrad Corp Ltd Metodo de purificacao do factor inibidor da leucemia (lif) e de composicoes farmaceuticas contendo polipeptidos com actividade do lif
AU609128B2 (en) * 1987-04-02 1991-04-26 Amrad Operations Pty. Limited Leukaemia-inhibitory factor
JPH03500481A (ja) * 1987-06-25 1991-02-07 イミュネックス・コーポレーション ウシ顆粒球マクロファージコロニー刺激因子
WO1989004173A1 (en) * 1987-11-12 1989-05-18 Schering Corporation Acceleration of bone formation with gm-csf
GB2212159B (en) * 1987-11-13 1992-01-22 British Bio Technology Synthetic gene for human granulocyte/macrophage colony stimulating factor.
AU615550B2 (en) * 1988-04-21 1991-10-03 Medvet Science Pty. Ltd. Human gm-csf variants
AU5355790A (en) * 1989-04-19 1990-11-16 Cetus Corporation Multifunctional m-csf proteins and genes encoding therefor
ATE103932T1 (de) * 1989-08-22 1994-04-15 Immunex Corp Fusionsprotein bestehend aus gm-csf und il-3.
NZ236819A (en) * 1990-02-03 1993-07-27 Max Planck Gesellschaft Enzymatic cleavage of fusion proteins; fusion proteins; recombinant dna and pharmaceutical compositions
US5270181A (en) * 1991-02-06 1993-12-14 Genetics Institute, Inc. Peptide and protein fusions to thioredoxin and thioredoxin-like molecules
AU7095796A (en) * 1995-09-28 1997-04-17 Otsuka Pharmaceutical Co., Ltd. Neutrophil chemotactic lymphokine, and drug and kit for the diagnosis of drug hypersensitive granulocytopenia containing the same
CN102917709B (zh) 2009-12-23 2018-04-24 格兰达利斯有限公司 弗林蛋白酶敲减和gm-csf增强的（fang）癌症疫苗
SG181881A1 (en) 2009-12-23 2012-07-30 Gradalis Inc Furin-knockdown bi-functional rna

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US4438032A (en) * 1981-01-30 1984-03-20 The Regents Of The University Of California Unique T-lymphocyte line and products derived therefrom
SE8300693L (sv) * 1983-02-09 1984-08-10 Sven Lofdahl Sett att framstella och isolera proteiner och polypeptider samt en hybridvektor for detta
IL71991A (en) * 1983-06-06 1994-05-30 Genentech Inc Preparation of human FGI and FGE in their processed form through recombinant AND tranology in prokaryotes
AU594014B2 (en) * 1984-03-21 1990-03-01 Research Corporation Technologies, Inc. Recombinant DNA molecules
ATE67244T1 (de) * 1984-07-06 1991-09-15 Sandoz Ag Herstellung und reinigung von lymphokinen.
ZA856108B (en) * 1984-10-29 1986-10-29 Immunex Corp Cloning of human granulocyte-macrophage colony simulating factor gene
AU588819B2 (en) * 1984-10-29 1989-09-28 Immunex Corporation Cloning of human granulocyte-macrophage colony stimulating factor gene
PT81513B (pt) * 1984-11-20 1988-03-03 Schering Biotech Corp Clones de dna codificando polipeptideos que apresentam actividade de factor de crescimento celular para granulocitos, madrofagos e eosinofilos humanos
JPS63502795A (ja) * 1985-10-03 1988-10-20 バイオジェン インコーポレイテッド 顆粒球‐マクロファージコロニー刺激因子‐様ポリペプチド、ｄｎａ配列、組換えｄｎａ分子並びに微生物細胞中でヒト顆粒球−マクロファ−ジコロニ−刺激因子−様ポリペプチドを高収量で生産する方法
DE3541856A1 (de) * 1985-11-27 1987-06-04 Hoechst Ag Eukaryotische fusionsproteine, ihre herstellung und verwendung sowie mittel zur durchfuehrung des verfahrens
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100448021B1 (ko) * 2001-12-28 2004-09-08 크레아젠 주식회사 마우스 과립구 대식세포 콜로니 촉진인자 발현벡터 ｐＧＭ-ＣＳＦ로 형질전환된 대장균 및 상기 마우스 과립구 대식세포 콜로니 촉진인자의 대량생산방법

Also Published As

Publication number Publication date
IE863335L (en) 1987-06-21
HU202584B (en) 1991-03-28
EP0228018B1 (de) 1992-01-02
DE3683267D1 (de) 1992-02-13
HUT44615A (en) 1988-03-28
AU601959B2 (en) 1990-09-27
ZA869557B (en) 1987-10-28
FI91168C (fi) 1994-05-25
FI865186A0 (fi) 1986-12-18
DK170346B1 (da) 1995-08-07
IE59779B1 (en) 1994-04-06
FI865186A (fi) 1987-06-22
FI91168B (fi) 1994-02-15
JPS62164695A (ja) 1987-07-21
KR940005585B1 (ko) 1994-06-21
ES2055686T3 (es) 1994-09-01
DK619086A (da) 1987-06-22
EP0228018A3 (en) 1987-12-02
IL81020A (en) 1995-11-27
CA1341197C (en) 2001-03-06
AU637139B2 (en) 1993-05-20
AU6675686A (en) 1987-06-25
IL81020A0 (en) 1987-03-31
ATE71144T1 (de) 1992-01-15
DE3545568A1 (de) 1987-07-16
NO177270C (no) 1995-08-16
NO865191D0 (no) 1986-12-19
NO177270B (no) 1995-05-08
DK619086D0 (da) 1986-12-19
JP2575367B2 (ja) 1997-01-22
PT83972A (de) 1987-01-01
GR3003999T3 (ko) 1993-03-16
EP0228018A2 (de) 1987-07-08
AU6268390A (en) 1990-12-13
PT83972B (pt) 1989-07-31
NO865191L (no) 1987-06-22

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