KR870700234A - 형질 전환된 효모균(yeats)에 의한 히로딘(Hirudin) 분비 및 발현 벡터(Vectors) - Google Patents

형질 전환된 효모균(yeats)에 의한 히로딘(Hirudin) 분비 및 발현 벡터(Vectors)

Info

Publication number
KR870700234A
KR870700234A KR860700971A KR860700971A KR870700234A KR 870700234 A KR870700234 A KR 870700234A KR 860700971 A KR860700971 A KR 860700971A KR 860700971 A KR860700971 A KR 860700971A KR 870700234 A KR870700234 A KR 870700234A
Authority
KR
South Korea
Prior art keywords
hirudin
yeast
gene
sequence
functional block
Prior art date
Application number
KR860700971A
Other languages
English (en)
Inventor
제라르 르와송
폴 톨스토셰브
이브 르모완느
쟝-삐에르 레콕
Original Assignee
에티엔느 아이 셍망
트랑스젠느 소시에떼 아노님
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 에티엔느 아이 셍망, 트랑스젠느 소시에떼 아노님 filed Critical 에티엔느 아이 셍망
Publication of KR870700234A publication Critical patent/KR870700234A/ko

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/815—Protease inhibitors from leeches, e.g. hirudin, eglin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/02—Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • C07K14/395—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts from Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Mycology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Hematology (AREA)
  • Diabetes (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Instructional Devices (AREA)
  • Glass Compositions (AREA)
  • Developing Agents For Electrophotography (AREA)

Abstract

내용 없음

Description

형질 전환된 효모균(yeats)에 의한 히로딘(Hirudin) 분비및 발현 벡터 (Vectors)
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음

Claims (31)

  1. 히루딘 또는 히루딘 변이체(H유전자)의 유전자와, 효모균에 의한 H유전자의 전사를 가능케 하는 정보를 담은 DNA시켄스(Str)를 최소한 포함하는 것을 특징으로 하며, 효모균으로부터 히루딘을 제조하는 것을 가능케 하는 기능을 가진 기능성 DNA블록.
  2. 청구범위 1항에 있어서, 최소한 시켄스-Str-Lex-Scl-H 유전자-를 갖는 것을 특징으로 하는 기능성 블륵:여기서 Lex는 유전자 제품의 분비를 얻기 위한 리더 시켄스임, Scl은 절단부위의 정보를 담은 DNA 시켄스임, 요소 Scl-H 유전자는 여러번 반복될 수 있음.
  3. 청구범위 2항에 있어서, 시켄스 Lex는 효모균의 α성 페로몬의 것임을 특징으로 하는 기능성 블록.
  4. 청구범위 1항 내지 3항중 어느 하나에 있어서, Scl은 3'단말의 H유전자를 앞서는 ATG코우돈을 포함하는 시켄스임을 특징으로 하는 기능성 블록.
  5. 청구범위 1항 내지 3항중 어느 하나에 있어서 Scl은 3'단말의 H유전자 앞에 Lys-Arg정보를 담은 두개의 코우돈을 포함하는 시켄스 임을 특징으로 하는 기능성 블록.
  6. 청구범위 1항 내지 3항중 어느 하나에 있어서, Scl은 3'단말에 Ser-Leu-Asp-Lys-Arg의 정보를 담은 다섯개의 코우돈을 포함하는 시켄스 임을 특징으로 하는 기능성블록.
  7. 청구범위 1힝 내지 6항중 어느 하나에 있어서, H유전자에는 효모균 단말 시켄스가 뒤따르는 것을 특징으로 하는 기능성 블륵.
  8. 청구범위 7항에 있어서, 효모균 단말 시켄스는 PGK유전자의 것임을 특징으로 하는 기능성 블록.
  9. 청구범위 1항 내지 8항중 어느 하나에 있어서, 이 블록은 다음 아미노산의 정보를 담은 최소한 한가지의 시켄스를 포함하는 것을 특징으로 하는 기능성 블록.
    1) Lys Arg Glu Ala Glu Ala Trp Leu Gln ValAsp Gly Ser Met……, 2) Lys Arg Glu Ala Glu Ala 히루딘……, 3) Lys Arg Glu Ala Glu Ala Lys Arg……, 4) Lys Arg Glu Ala Glu Lla Ser Leu Asp Lys Arg 히루딘……, 또는, 5) Lys Arg 히루딘…….
  10. 효모균내의 복제 근원중 최소한 하나 및 청구범위 1항 내지 9항중 어느 하나에 의한 기능성 블록을 포함하는 것을 특징으로 하는 효모균에 의한 히루딘 분비에 필요한 요소를 포함하는 플라스미드.
  11. 청구범위 10항에 있어서, 복제의 근원은 2μ플라스미드의 것임을 특징으로 하는 플라스미드.
  12. 청구범위 10항 및 11항중 어느 하나에 있어서, 추가로 선택특성을 포함하는 것을 특징으로 하는 플라스미드.
  13. 청구범위 12항에 있어서, 선택 특성은 URA 3유전자에 의해 주어지는 것을 특징으로 하는 플라스미드.
  14. 상기 발현 블록(청구범위 1항 내지 8항중 어느 하나에 의한 것), 또는 청구 범위 10항 내지 13항중 어느 하나에 의한 플라스미드에 의해 현질 전환된 효모균.
  15. 청구범위 14항에 있어서, 사카로마이스 속(屬)의 효모균임을 특징으로 하는 효모균.
  16. 청구범위 15항에 있어서, 효모균은 Matα성형(性型)임을 특징으로 하는 효모균.
  17. 청구범위 14항 내지 16항중 어느 하나에 있어서, 효모균은 S. 세레비시아에 균주임을 특징으로 하는 효모균.
  18. 배양 매질 내에서 청구범위 14항 내지 17항중 어느 하나에 의한 효모균을 발효시키고, 성숙한 형태로, 또는 생체내에서 성숙화 될 수 있는 히루딘 전구 물질의 상태로 배양 매질 내에서 히루딘을 회수하는 히루딘의 제조방법.
  19. 청구범위 18항에 있어서 히루딘은 배양 매질 내에서 회수되는 것을 특징으로 하는 제조방법.
  20. 청구범위 19항에 있어서, Scl에 해당하는 시켄스를 화학적으로 또는 효소에 의하여 절단할 때 생기는 전구 물체로부터 성숙한 히루딘을 얻는 것을 특징으로 하는 제조방법.
  21. 청구범위 20항에 있어서, 메티오닌부위를 화학적으로 절단하여 성숙한 히루딘을 얻는 것을 특징으로 하는 제조방법.
  22. 청구범위 21항에 있어서, 전구 물체의 Lys-Arg 또는 Ser-Leu-Asp-Lys-Arg시켄스 이후의 부위를 효소로 절단하여 성숙한 히루딘을 얻는 것을 특징으로 하는 제조 방법.
  23. 청구범위 18항 내지 20항중 어느 하나에 있어서, 전구 물체를 절단하여 얻은 히루딘은 변성 상태로 부터 환원시키는 것을 특징으로 하는 제조방법.
  24. 전항(18항 내지 23항중 어느 하나)에 의한 방법을 수행하여 얻은 히루딘.
  25. 의약품으로서의 청구범위 24항에 의한 히루딘의 용도.
  26. 인체 또는 동물내에 형액응결 검출제로서의 청구범위 24항에 의한 히루딘의 용도.
  27. 신체 외적 혈액순환 계통의 최소한 일부분이 청구범위 24항에 의한 히루딘으로 표면 도포 되었을때 신체외적 혈액순환 계통에의 히루딘 용도.
  28. 히루딘을 방출하는 의약품으로서의 청구범위 14항 내지 17항중 어느 하나에 의한 효모균의 용도.
  29. 전(항청구범위 24항)에 의한 히루딘을 포함하는 항응혈제.
  30. 표지된, 청구범위 24항에 의한 히루딘.
  31. 생체내의 이미징(Imaging)에 사용되는, 청구범위 30항에 의한 히루딘의 용도.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR860700971A 1985-05-02 1986-05-02 형질 전환된 효모균(yeats)에 의한 히로딘(Hirudin) 분비 및 발현 벡터(Vectors) KR870700234A (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
FR8506672A FR2593518B1 (fr) 1985-05-02 1985-05-02 Vecteurs d'expression et de secretion de l'hirudine par les levures transformees
FR8506672 1985-05-02
PCT/FR1986/000153 WO1986006406A1 (fr) 1985-05-02 1986-05-02 Vecteurs d'expression et de secretion de l'hirudine par les levures transformees

Publications (1)

Publication Number Publication Date
KR870700234A true KR870700234A (ko) 1987-05-30

Family

ID=9318888

Family Applications (1)

Application Number Title Priority Date Filing Date
KR860700971A KR870700234A (ko) 1985-05-02 1986-05-02 형질 전환된 효모균(yeats)에 의한 히로딘(Hirudin) 분비 및 발현 벡터(Vectors)

Country Status (23)

Country Link
EP (1) EP0200655B1 (ko)
JP (2) JP2580141B2 (ko)
KR (1) KR870700234A (ko)
AT (1) ATE121778T1 (ko)
AU (1) AU600667B2 (ko)
BG (1) BG49717A3 (ko)
CA (1) CA1341501C (ko)
CZ (1) CZ282928B6 (ko)
DE (1) DE3650306T2 (ko)
DK (1) DK174837B1 (ko)
ES (1) ES8703930A1 (ko)
FI (1) FI104635B (ko)
FR (2) FR2593518B1 (ko)
HK (1) HK1006183A1 (ko)
HU (1) HU202919B (ko)
IE (1) IE67136B1 (ko)
MC (1) MC1785A1 (ko)
NO (1) NO302375B1 (ko)
PL (1) PL155074B1 (ko)
PT (1) PT82490B (ko)
RU (1) RU1774950C (ko)
WO (1) WO1986006406A1 (ko)
ZA (1) ZA863261B (ko)

Families Citing this family (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3738541A1 (de) * 1987-11-13 1989-05-24 Hoechst Ag Verfahren zur isolierung und reinigung von hirudin
FR2593518B1 (fr) * 1985-05-02 1989-09-08 Transgene Sa Vecteurs d'expression et de secretion de l'hirudine par les levures transformees
GB8530631D0 (en) 1985-12-12 1986-01-22 Ciba Geigy Ag Thrombin inhibitors
MY101203A (en) * 1985-12-12 1991-08-17 Ucp Gen Pharma Ag Production of thrombin iinhibitors.
MC1834A1 (fr) * 1986-07-10 1988-06-03 Transgene Sa Bloc fonctionnel d'adn et plasmide codant pour l'hirudine,levure transformee,procede de preparation de l'hirudine,hirudine obtenue et son utilisation
FR2607516B2 (fr) * 1986-12-01 1989-12-22 Transgene Sa Vecteurs perfectionnes d'expression d'un variant de l'hirudine dans les levures, procede et produit obtenu
FR2607517B2 (fr) * 1986-12-01 1989-12-22 Transgene Sa Vecteurs d'expression de variants de l'hirudine dans les levures, procede et produit obtenu
FR2611723B1 (fr) * 1987-02-27 1989-09-08 Transgene Sa Vecteur d'expression d'adn recombinant codant pour l'hirudine, cultures de cellules de mammiferes infectees ou transformees, procede de preparation de l'hirudine et proteine obtenue
EP0371041A1 (en) * 1987-06-24 1990-06-06 Novo Nordisk A/S A process for preparing a protein or polypeptide, a dna sequence coding for the polypeptide, a microorganism containing the dna sequence as well as the polypeptide and its use as a pharmaceutical preparation
US5256559A (en) * 1988-03-04 1993-10-26 Biogen, Inc. Methods and compositions for inhibiting platelet aggregation
DE3819079A1 (de) * 1988-06-04 1989-12-07 Hoechst Ag Hirudin-derivate mit verzoegerter wirkung
AU633020B2 (en) * 1988-07-23 1993-01-21 Novozymes Delta Limited New secretory leader sequences
DE3835815A1 (de) * 1988-10-21 1990-04-26 Hoechst Ag Neue isohirudine
CA2000887A1 (en) * 1988-11-01 1990-05-01 Cecilia S.L. Ku Thromboresistant materials and methods for making same
US5053453A (en) * 1988-11-01 1991-10-01 Baxter International Inc. Thromboresistant materials and methods for making same
FR2646437B1 (fr) * 1989-04-28 1991-08-30 Transgene Sa Nouvelles sequences d'adn, leur application en tant que sequence codant pour un peptide signal pour la secretion de proteines matures par des levures recombinantes, cassettes d'expression, levures transformees et procede de preparation de proteines correspondant
DE4009268A1 (de) * 1990-03-22 1991-09-26 Consortium Elektrochem Ind Sekretion von hirudinderivaten
US6514730B1 (en) 1991-03-21 2003-02-04 Consortium für elektrochemische Industrie GmbH Secretion of hirudin derivatives
US5837808A (en) * 1991-08-20 1998-11-17 Baxter International Inc. Analogs of hirudin
DE4140381A1 (de) * 1991-12-07 1993-06-09 Hoechst Ag, 6230 Frankfurt, De Neue synthetische isohirudine mit verbesserter stabilitaet
SG49859A1 (en) * 1992-09-04 2002-03-19 Ucp Gen Pharma Ag Process for the production of protease inhibitors
US6500645B1 (en) 1994-06-17 2002-12-31 Novo Nordisk A/S N-terminally extended proteins expressed in yeast
ZA954983B (en) * 1994-06-17 1996-02-14 Novo Nordisk As N-terminally extended proteins expressed in yeast
DE19529997C1 (de) 1995-08-16 1997-04-10 Hoechst Ag Verfahren zur Inaktivierung von Carboxypeptidase Y in hirudinhaltigen Kulturbrühen
DE19543737A1 (de) * 1995-11-24 1997-05-28 Hoechst Ag Verfahren zur Ultrafiltration von Peptide oder Proteine enthaltender biologischer Matrices
DE19544233A1 (de) * 1995-11-28 1997-06-05 Hoechst Ag Verfahren zur Nutzung des Hefe-ADH II-Promotorsystems zur biotechnologischen Produktion heterologer Proteine in hohen Ausbeuten
ZA9610456B (en) * 1995-12-20 1997-06-20 Novo Nordisk As N-terminally extended proteins expressed in yeast
EP0787569B1 (en) 1996-01-31 2002-10-02 Sumitomo Bakelite Company Limited Method of producing epoxy resin-encapsulated semiconductor device
US6265204B1 (en) * 1997-01-17 2001-07-24 Genencor International, Inc. DNA sequences, vectors, and fusion polypeptides for secretion of polypeptides in filamentous fungi
US7638618B2 (en) 2001-02-20 2009-12-29 Sanofi-Aventis Deutschland Gmbh Nucleic acids encoding a hirudin and pro-insulin as superscretable peptides and for parallel improvement of the exported forms of one or more polypeptides of interest
US7202059B2 (en) 2001-02-20 2007-04-10 Sanofi-Aventis Deutschland Gmbh Fusion proteins capable of being secreted into a fermentation medium
DE10149678A1 (de) 2001-10-09 2009-03-12 Novel Science International Gmbh Organische Verbindungen mit biologischer Wirkung als Thrombinhemmer und ihre Verwendung
DE10301255A1 (de) 2003-01-15 2004-07-29 Cpi Creative Pharma International Gmbh Organische Verbindungen mit biologischer Wirkung als Thrombinhemmer und ihre Verwendung
US7795205B2 (en) 2004-04-12 2010-09-14 Canyon Pharmaceuticals, Inc. Methods for effecting regression of tumor mass and size in a metastasized pancreatic tumor

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3382547D1 (de) * 1983-01-12 1992-05-27 Chiron Corp Sekretorische expression in eukaryoten.
AU572125B2 (en) * 1983-03-17 1988-05-05 Mabco Limited Monoclonal antibodies with specificity for crosslinked fibrin and their diagnotic uses
JPS60501140A (ja) * 1983-04-22 1985-07-25 アムジエン 酵母による外因性ポリペプチドの分泌
JPS6041487A (ja) * 1983-04-25 1985-03-05 ジエネンテツク・インコ−ポレイテツド 酵母発現系でのアルフア因子配列の使用
NZ207926A (en) * 1983-04-25 1988-04-29 Genentech Inc Use of yeast #a#-factor to assist in expression of proteins heterologus to yeast
JPS59227899A (ja) * 1983-05-25 1984-12-21 チロン・コ−ポレイシヨン 成長ホルモン放出因子の製造方法
CA1341417C (fr) * 1984-03-27 2003-01-21 Paul Tolstoshev Vecteurs d'expression de l'hirudine, cellules transformees et procede de preparation de l'hirudine
ATE64956T1 (de) * 1984-06-14 1991-07-15 Ciba Geigy Ag Verfahren zur herstellung von thrombininhibitoren.
DE3429430A1 (de) * 1984-08-10 1986-02-20 Hoechst Ag, 6230 Frankfurt Gentechnologisches verfahren zur herstellung von hirudin und mittel zur durchfuehrung dieses verfahrens
DE3445517C2 (de) * 1984-12-13 1993-11-18 Ciba Geigy Für ein Hirudin-ähnliches Protein codierende DNA-Sequenz und Verfahren zur Herstellung eines Hirudin-ähnlichen Proteins
FR2593518B1 (fr) * 1985-05-02 1989-09-08 Transgene Sa Vecteurs d'expression et de secretion de l'hirudine par les levures transformees

Also Published As

Publication number Publication date
FI865303A (fi) 1986-12-23
BG49717A3 (en) 1992-01-15
FI104635B (fi) 2000-03-15
HK1006183A1 (en) 1999-02-12
FR2593518B1 (fr) 1989-09-08
CZ282928B6 (cs) 1997-11-12
DK174837B1 (da) 2003-12-15
WO1986006406A1 (fr) 1986-11-06
AU5778786A (en) 1986-11-18
ES8703930A1 (es) 1987-03-16
CA1341501C (fr) 2006-03-14
DK634386D0 (da) 1986-12-30
IE861183L (en) 1986-11-02
PT82490A (fr) 1986-05-01
JPH09103295A (ja) 1997-04-22
PT82490B (pt) 1988-03-03
ATE121778T1 (de) 1995-05-15
HUT42131A (en) 1987-06-29
JPS62502661A (ja) 1987-10-15
IE67136B1 (en) 1996-03-06
HU202919B (en) 1991-04-29
JP2580141B2 (ja) 1997-02-12
FI865303A0 (fi) 1986-12-23
EP0200655B1 (fr) 1995-04-26
FR2601383B2 (fr) 1989-12-22
DE3650306T2 (de) 1995-10-12
ZA863261B (en) 1986-12-30
PL155074B1 (en) 1991-10-31
DE3650306D1 (de) 1995-06-01
ES555121A0 (es) 1987-03-16
RU1774950C (ru) 1992-11-07
FR2593518A1 (fr) 1987-07-31
JP2779930B2 (ja) 1998-07-23
NO302375B1 (no) 1998-02-23
FR2601383A2 (fr) 1988-01-15
AU600667B2 (en) 1990-08-23
CZ321786A3 (en) 1997-07-16
NO865336L (no) 1987-03-02
EP0200655A1 (fr) 1986-11-05
MC1785A1 (fr) 1987-09-02
DK634386A (da) 1986-12-30

Similar Documents

Publication Publication Date Title
KR870700234A (ko) 형질 전환된 효모균(yeats)에 의한 히로딘(Hirudin) 분비 및 발현 벡터(Vectors)
Mizuno et al. A novel protease from yeast with specificity towards paired basic residues
CA1341203C (en) Eukaryotic fusion proteins, containing interleukin-2 sequences
GB2160206B (en) Dna sequence coding for an enzyme cleavage site
US4892743A (en) Novel hybrid interferon species
DK728588A (da) Gaercelle omfattende en dna-konstruktion, der tilvejebringer ekspression og secernering af et ikke-gaerprotein, samt dobbeltstrenget dna-molekyle
IE810433L (en) A specific cleavage linker
DE68922917D1 (de) KEX2-Endoprotease und Verfahren zu ihrer Herstellung.
Suzuki et al. A rapid and large-scale isolation of renin from mouse submaxillary gland by pepstatin-aminohexyl-agarose affinity chromatography
KR880012758A (ko) 폴리펩타이드의 유전공학적 제조방법
EP0276778A3 (en) High level expression in e. coli of soluble mature hil-1beta and derivatives with altered biological activity
IL92178A (en) Process for preparing pre-insulin in streptomycetes
JP2696316B2 (ja) ヒルジンの発現のためのベクター、形質転換細胞及びヒルジンの製造方法
US5821079A (en) Vectors for the expression and secretion of hirudin by transformed yeasts
US5316919A (en) Method of producing 2 KD to 10 KD peptides having no L-methionine residue
Okada et al. Novel Leu-Lys-specific peptidase (Leulysin) produced by gel-entrapped yeast cells
SE8102267L (sv) Forfarande for framstellning av ej koagulerbart blod medelst proteolytiska enzymer och proteinkoncentrat framstellt derur
YAGI et al. Primary structure of the N-terminal portion of D-amino acid oxidase
ATE160586T1 (de) Pharmazeutische zusammensetzungen die superoxiddismutase aus bacillus stearothermophilus und bacillus caldotenax enthalten
JPH06228000A (ja) 気道閉塞改善剤
JPH0782168A (ja) 間質性肺炎の治療剤

Legal Events

Date Code Title Description
A201 Request for examination
E902 Notification of reason for refusal
E601 Decision to refuse application
J2X1 Appeal (before the patent court)

Free format text: APPEAL AGAINST DECISION TO DECLINE REFUSAL

J2X2 Appeal (before the supreme court)

Free format text: APPEAL BEFORE THE SUPREME COURT FOR APPEAL AGAINST DECISION TO DECLINE REFUSAL