KR20090075825A - 세린 프로테아제의 다중 돌연변이 변이체 - Google Patents

세린 프로테아제의 다중 돌연변이 변이체 Download PDF

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Publication number
KR20090075825A
KR20090075825A KR1020097007937A KR20097007937A KR20090075825A KR 20090075825 A KR20090075825 A KR 20090075825A KR 1020097007937 A KR1020097007937 A KR 1020097007937A KR 20097007937 A KR20097007937 A KR 20097007937A KR 20090075825 A KR20090075825 A KR 20090075825A
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South Korea
Prior art keywords
protease
variant
amino acid
acid sequence
cleaning
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Ceased
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KR1020097007937A
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English (en)
Korean (ko)
Inventor
볼프강 애레
데이비드 에이 에스텔
로날뒤스 더블유.제이. 호메스
브라이언 이 존스
마르크 콜크만
크리스 레플랑
히로시 오
애이루카란 제이 폴로즈
앤드류 쇼
데르 클라에이 빌헬뮈스 에이 에이치 반
마레베이크 레오 반
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다니스코 유에스 인크.
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Application filed by 다니스코 유에스 인크. filed Critical 다니스코 유에스 인크.
Publication of KR20090075825A publication Critical patent/KR20090075825A/ko
Ceased legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/48Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/189Enzymes
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K50/00Feeding-stuffs specially adapted for particular animals
    • A23K50/70Feeding-stuffs specially adapted for particular animals for birds
    • A23K50/75Feeding-stuffs specially adapted for particular animals for birds for poultry
    • CCHEMISTRY; METALLURGY
    • C11ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11DDETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16Organic compounds
    • C11D3/38Products with no well-defined composition, e.g. natural products
    • C11D3/386Preparations containing enzymes, e.g. protease or amylase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52Genes encoding for enzymes or proenzymes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/96Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
    • DTEXTILES; PAPER
    • D06TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
    • D06MTREATMENT, NOT PROVIDED FOR ELSEWHERE IN CLASS D06, OF FIBRES, THREADS, YARNS, FABRICS, FEATHERS OR FIBROUS GOODS MADE FROM SUCH MATERIALS
    • D06M16/00Biochemical treatment of fibres, threads, yarns, fabrics, or fibrous goods made from such materials, e.g. enzymatic
    • D06M16/003Biochemical treatment of fibres, threads, yarns, fabrics, or fibrous goods made from such materials, e.g. enzymatic with enzymes or microorganisms
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y304/00Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21Serine endopeptidases (3.4.21)

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Polymers & Plastics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Animal Husbandry (AREA)
  • Birds (AREA)
  • Food Science & Technology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • General Chemical & Material Sciences (AREA)
  • Textile Engineering (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Detergent Compositions (AREA)
  • Fodder In General (AREA)
KR1020097007937A 2006-10-19 2007-08-29 세린 프로테아제의 다중 돌연변이 변이체 Ceased KR20090075825A (ko)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US58333406A 2006-10-19 2006-10-19
US11/583,334 2006-10-19
US11/809,104 2007-05-31
US11/809,104 US7985569B2 (en) 2003-11-19 2007-05-31 Cellulomonas 69B4 serine protease variants

Related Child Applications (1)

Application Number Title Priority Date Filing Date
KR1020147028525A Division KR20140136033A (ko) 2006-10-19 2007-08-29 세린 프로테아제의 다중 돌연변이 변이체

Publications (1)

Publication Number Publication Date
KR20090075825A true KR20090075825A (ko) 2009-07-09

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KR1020097007937A Ceased KR20090075825A (ko) 2006-10-19 2007-08-29 세린 프로테아제의 다중 돌연변이 변이체
KR1020147028525A Ceased KR20140136033A (ko) 2006-10-19 2007-08-29 세린 프로테아제의 다중 돌연변이 변이체

Family Applications After (1)

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Country Status (9)

Country Link
US (4) US7985569B2 (https=)
EP (1) EP2084273A1 (https=)
JP (1) JP5775666B2 (https=)
KR (2) KR20090075825A (https=)
BR (1) BRPI0718483A2 (https=)
CA (1) CA2667043A1 (https=)
MX (1) MX2009003936A (https=)
RU (1) RU2558261C2 (https=)
WO (1) WO2008048392A1 (https=)

Families Citing this family (62)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE450606T1 (de) * 2002-01-16 2009-12-15 Genencor Int Mehrfachsubstituierte proteasevarianten
MXPA04006811A (es) * 2002-01-16 2004-10-11 Genencor Int Variantes de proteasa substituidas con multiplos.
JP5785686B2 (ja) * 2006-06-23 2015-09-30 ダニスコ・ユーエス・インク、ジェネンコー・ディビジョン 複数の性質をエンジニアリングするための部位評価ライブラリーを用いた、配列と活性との関係の系統的な評価
WO2008044016A1 (en) * 2006-10-14 2008-04-17 Reckitt Benckiser N.V. Composition
EP2118121A1 (en) * 2007-03-01 2009-11-18 Novo Nordisk A/S Expression of proteins in e. coli
CN101889084A (zh) * 2007-12-04 2010-11-17 诺维信公司 供药学应用的蛋白酶变体
US20090209447A1 (en) * 2008-02-15 2009-08-20 Michelle Meek Cleaning compositions
MX2010009457A (es) * 2008-02-29 2010-09-24 Procter & Gamble Composicion detergente que comprende lipasa.
WO2009107091A2 (en) * 2008-02-29 2009-09-03 The Procter & Gamble Company Detergent composition comprising lipase
EP2100947A1 (en) 2008-03-14 2009-09-16 The Procter and Gamble Company Automatic dishwashing detergent composition
US20090233830A1 (en) 2008-03-14 2009-09-17 Penny Sue Dirr Automatic detergent dishwashing composition
EP2166073A1 (en) * 2008-09-23 2010-03-24 The Procter & Gamble Company Cleaning composition
EP2166075A1 (en) * 2008-09-23 2010-03-24 The Procter and Gamble Company Cleaning composition
US8096062B1 (en) * 2008-10-08 2012-01-17 Bellen Mark L Towel drying system
US20100125046A1 (en) 2008-11-20 2010-05-20 Denome Frank William Cleaning products
US20140014137A1 (en) 2009-09-18 2014-01-16 Ecolab Usa Inc. Treatment of non-trans fats with acidic tetra sodium l-glutamic acid, n, n-diacetic acid (glda)
MX2012006616A (es) 2009-12-09 2012-06-21 Procter & Gamble Productos para el cuidado de las telas y el hogar.
EP2380478A1 (en) 2010-04-23 2011-10-26 The Procter & Gamble Company Automatic dishwashing product
ES2682051T3 (es) 2010-04-23 2018-09-18 The Procter & Gamble Company Composición detergente
ES2565192T3 (es) 2010-04-23 2016-04-01 The Procter & Gamble Company Método para perfumar
ES2533368T3 (es) 2010-04-23 2015-04-09 The Procter & Gamble Company Producto para lavavajillas
EP2383329A1 (en) 2010-04-23 2011-11-02 The Procter & Gamble Company Particle
EP2380962B1 (en) 2010-04-23 2016-03-30 The Procter and Gamble Company Particle
EP3095861B1 (en) 2010-05-06 2019-06-26 The Procter and Gamble Company Consumer products with protease variants
FI123425B (fi) * 2011-03-31 2013-04-30 Ab Enzymes Oy Proteaasientsyymi ja sen käytöt
ES2834102T3 (es) 2011-06-30 2021-06-16 Novozymes As Variantes de alfa-amilasa
BR112014017919A2 (pt) 2012-01-26 2017-06-27 Novozymes As uso de um poliptídeo variante, composição, polinucleotídeo isolado, constructo de ácido nucleico ou vetor de expressão, célula hospedeira de expressão recombinante, métodos de produção do pilipeptídeo, para melhoria do valor nutricional de uma ração animal, e para o tratamento de proteínas, planta, parte de planta ou célula de planta transgênica, aditivo de ração animal, ração animal, e, composição de ração animal ou detergente
ES2599504T3 (es) 2012-05-30 2017-02-02 Clariant International Ltd Utilización de N-metil-N-acil-glucaminas como agentes solubilizantes
IN2014DN09937A (https=) 2012-05-30 2015-08-14 Clariant Int Ltd
EP2866895B1 (de) * 2012-05-30 2016-08-31 Clariant International Ltd Tensidlösungen mit n-methyl-n-oleylglucamin und n-methyl-n-c12-c14-acylglucamin
AU2013279440B2 (en) 2012-06-20 2016-10-06 Novozymes A/S Use of polypeptides having protease activity in animal feed and detergents
US10253281B2 (en) 2012-08-20 2019-04-09 Ecolab Usa Inc. Method of washing textile articles
DE102012021647A1 (de) 2012-11-03 2014-05-08 Clariant International Ltd. Wässrige Adjuvant-Zusammensetzungen
AU2014214032A1 (en) 2013-02-06 2015-07-09 Novozymes A/S Use of polypeptides having protease activity in animal feed
US20160068620A1 (en) 2013-03-15 2016-03-10 Lubrizol Advanced Materials, Inc. Itaconic Acid Polymers
WO2015042013A1 (en) 2013-09-18 2015-03-26 Lubrizol Advanced Materials, Inc. Stable linear polymers
BR112016021154A2 (pt) 2014-03-14 2021-08-24 Lubrizol Advanced Materials, Inc. Polímeros e copolímeros de ácido itacônico
DE102014005771A1 (de) 2014-04-23 2015-10-29 Clariant International Ltd. Verwendung von wässrigen driftreduzierenden Zusammensetzungen
CN106687572A (zh) 2014-09-10 2017-05-17 巴斯夫欧洲公司 包封清洁组合物
BR112017009320A2 (pt) 2014-11-04 2017-12-19 Novozymes As ração animal ou aditivo de ração animal, uso de ração animal ou aditivo de ração animal, e, métodos para preparação de uma ração animal, para melhoria do valor nutricional de uma ração animal, para tratamento de proteínas aumento da digestibilidade e/ou solubilidade da proteína, para melhoria de um ou mais parâmetros de desempenho em um animal e para produção de um polipeptídeo.
EP3034596B2 (en) 2014-12-17 2021-11-10 The Procter & Gamble Company Detergent composition
EP3034590A1 (en) 2014-12-17 2016-06-22 The Procter and Gamble Company Method of automatic dishwashing
EP3034591A1 (en) 2014-12-17 2016-06-22 The Procter and Gamble Company Method of automatic dishwashing
ES2733300T3 (es) 2014-12-17 2019-11-28 Procter & Gamble Composición detergente
EP3034592A1 (en) 2014-12-17 2016-06-22 The Procter and Gamble Company Method of automatic dishwashing
EP3034597A1 (en) 2014-12-17 2016-06-22 The Procter and Gamble Company Detergent composition
EP3034589A1 (en) 2014-12-17 2016-06-22 The Procter and Gamble Company Detergent composition
ES2795009T3 (es) * 2015-01-29 2020-11-20 Ecolab Usa Inc Método para el tratamiento de manchas en textiles
EP3259350A4 (en) 2015-02-16 2018-11-07 Ozymes Multi-domain enzymes having cutinase activity, compositions comprising same and uses thereof
WO2016140703A1 (en) 2015-03-05 2016-09-09 Crossford International, Llc Systems and methods for tabletized tube cleaning
DE102015219608B4 (de) 2015-10-09 2018-05-03 Clariant International Ltd Universelle Pigmentdispersionen auf Basis von N-Alkylglukaminen
DE102015219651A1 (de) 2015-10-09 2017-04-13 Clariant International Ltd. Zusammensetzungen enthaltend Zuckeramin und Fettsäure
US20190085273A1 (en) 2016-03-09 2019-03-21 Basf Se Encapsulated laundry cleaning composition
KR101978828B1 (ko) * 2016-03-21 2019-05-15 주식회사 엘지생활건강 바이오필름 제거용 조성물
DE102016207877A1 (de) 2016-05-09 2017-11-09 Clariant International Ltd Stabilisatoren für Silikatfarben
MX2019007335A (es) * 2016-12-21 2019-09-09 Dupont Nutrition Biosci Aps Métodos para usar serina proteasas termoestables.
KR102316445B1 (ko) * 2019-11-29 2021-10-26 씨제이제일제당 주식회사 신규 세린 프로테아제 변이체
KR102613549B1 (ko) 2021-03-12 2023-12-13 씨제이제일제당 주식회사 신규 세린 프로테아제 변이체
JP7842216B2 (ja) 2021-12-16 2026-04-07 ザ プロクター アンド ギャンブル カンパニー プロテアーゼを含む自動食器洗浄組成物
CN115717135B (zh) * 2022-08-18 2024-07-16 天津科技大学 一种耐热木糖苷酶突变体及其制备
KR20240175756A (ko) * 2023-06-13 2024-12-23 씨제이제일제당 (주) 신규 세린 프로테아제 변이체
KR20250054900A (ko) * 2023-10-16 2025-04-24 씨제이제일제당 (주) 신규 세린 프로테아제 변이체

Family Cites Families (110)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3840433A (en) 1968-09-23 1974-10-08 Novo Terapeutisk Labor As Dehairing of leather
GB1296839A (https=) 1969-05-29 1972-11-22
GB1372034A (en) 1970-12-31 1974-10-30 Unilever Ltd Detergent compositions
ES421535A1 (es) 1973-01-13 1976-06-16 Roehm Gmbh Procedimiento para la preparacion de pellejos dispuestos para curtir a partir de cuero y pieles de origen animal.
DE3005845A1 (de) 1980-02-16 1981-09-03 Boehringer Mannheim Gmbh, 6800 Mannheim Aminosaeure- und peptidester von leuko-indoanilinen, verfahren zu deren herstellung sowie diese verbindungen enthaltende mittel zum nachweis proteolytischer enzyme
DK187280A (da) 1980-04-30 1981-10-31 Novo Industri As Ruhedsreducerende middel til et fuldvaskemiddel fuldvaskemiddel og fuldvaskemetode
GR76237B (https=) 1981-08-08 1984-08-04 Procter & Gamble
JPS58144105A (ja) 1982-02-12 1983-08-27 Kurabo Ind Ltd スケ−ル除去獣毛繊維の製法
US4760025A (en) 1984-05-29 1988-07-26 Genencor, Inc. Modified enzymes and methods for making same
US5972682A (en) 1984-05-29 1999-10-26 Genencor International, Inc. Enzymatically active modified subtilisins
US5801038A (en) 1984-05-29 1998-09-01 Genencor International Inc. Modified subtilisins having amino acid alterations
US5264366A (en) 1984-05-29 1993-11-23 Genencor, Inc. Protease deficient bacillus
US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
DK154572C (da) 1985-08-07 1989-04-24 Novo Industri As Enzymatisk detergentadditiv, detergent og fremgangsmaade til vask af tekstiler
JPH0697997B2 (ja) 1985-08-09 1994-12-07 ギスト ブロカデス ナ−ムロ−ゼ フエンノ−トチヤツプ 新規の酵素的洗浄剤添加物
US5364770A (en) 1985-08-29 1994-11-15 Genencor International Inc. Heterologous polypeptides expressed in aspergillus
US5766912A (en) 1986-03-17 1998-06-16 Novo Nordisk A/S Humicola lipase produced in aspergillus
DK122686D0 (da) 1986-03-17 1986-03-17 Novo Industri As Fremstilling af proteiner
US5322770A (en) 1989-12-22 1994-06-21 Hoffman-Laroche Inc. Reverse transcription with thermostable DNA polymerases - high temperature reverse transcription
ATE110768T1 (de) 1986-08-29 1994-09-15 Novo Nordisk As Enzymhaltiger reinigungsmittelzusatz.
AU620326B2 (en) 1987-02-27 1992-02-20 Dsm Ip Assets B.V. Stable gene amplification in chromosomal dna of prokaryotic microorganisms
BR8805647A (pt) 1987-02-27 1989-10-31 Gist Brocades Nv Processo para clonagem molecular e expressao de genes que codificam para enzimas proteoliticas
EP0322429B1 (en) 1987-05-29 1994-10-19 Genencor International, Inc. Cutinase cleaning composition
US5314692A (en) 1987-08-24 1994-05-24 Cultor Ltd. Enzyme premix for feed and method
DE3854249T2 (de) 1987-08-28 1996-02-29 Novonordisk As Rekombinante Humicola-Lipase und Verfahren zur Herstellung von rekombinanten Humicola-Lipasen.
WO1989004552A1 (en) 1987-10-30 1989-05-18 Lsi Logic Corporation Method and means of fabricating a semiconductor device package
EP0471265B1 (en) 1988-01-07 1995-10-25 Novo Nordisk A/S Specific protease
US5324653A (en) 1988-02-11 1994-06-28 Gist-Brocades N.V. Recombinant genetic means for the production of serine protease muteins
JP3079276B2 (ja) 1988-02-28 2000-08-21 天野製薬株式会社 組換え体dna、それを含むシュードモナス属菌及びそれを用いたリパーゼの製造法
US4977252A (en) 1988-03-11 1990-12-11 National Starch And Chemical Investment Holding Corporation Modified starch emulsifier characterized by shelf stability
CA1333777C (en) 1988-07-01 1995-01-03 Randy M. Berka Aspartic proteinase deficient filamentous fungi
DE3841152A1 (de) 1988-12-07 1990-06-13 Hoechst Ag Verwendung von bakterienlysierendem enzymprodukt als zusatzstoff zur verbesserung der futterverwertung in der tierproduktion
WO1990009446A1 (en) 1989-02-17 1990-08-23 Plant Genetic Systems N.V. Cutinase
DK158989D0 (da) 1989-03-31 1989-03-31 Novo Industri As Fremgangsmaade til enzymatisk afhaaring af huder eller skind
DE3922748B4 (de) 1989-07-11 2006-01-05 Röhm GmbH & Co. KG Enzymatisches Weichverfahren
EP0528828B2 (de) 1990-04-14 1997-12-03 Genencor International GmbH Alkalische bacillus-lipasen, hierfür codierende dna-sequenzen sowie bacilli, die diese lipasen produzieren
US5354559A (en) 1990-05-29 1994-10-11 Grain Processing Corporation Encapsulation with starch hydrolyzate acid esters
US5401657A (en) 1990-08-06 1995-03-28 Gist-Brocades, N.V. Gram-positive alkaliphilic microorganisms
DE69129988T2 (de) 1990-09-13 1999-03-18 Novo Nordisk A/S, Bagsvaerd Lipase-varianten
DE4035839A1 (de) 1990-11-10 1992-05-14 Roehm Gmbh Protease als wirkenzym enthaltende, tensidfreie feste enzympraeparate
DE69133035T2 (de) 1991-01-16 2003-02-13 The Procter & Gamble Company, Cincinnati Kompakte Waschmittelzusammensetzungen mit hochaktiven Cellulasen
DE4109826A1 (de) 1991-03-26 1992-11-05 Roehm Gmbh Enzymatisch unterstuetze aescher- und beizverfahren
JP2905311B2 (ja) 1991-04-24 1999-06-14 ユニチカ株式会社 獣毛繊維製品の防縮加工方法
US5340735A (en) 1991-05-29 1994-08-23 Cognis, Inc. Bacillus lentus alkaline protease variants with increased stability
US5646028A (en) 1991-06-18 1997-07-08 The Clorox Company Alkaline serine protease streptomyces griseus var. alkaliphus having enhanced stability against urea or guanidine
FI952648L (fi) 1992-12-01 1995-07-28 Novo Nordisk As Entsymaattisten reaktioiden tehostaminen
AU6029894A (en) 1993-01-18 1994-08-15 Procter & Gamble Company, The Machine dishwashing detergent compositions
DK48693D0 (da) 1993-04-30 1993-04-30 Novo Nordisk As Enzym
DK77393D0 (da) 1993-06-29 1993-06-29 Novo Nordisk As Aktivering af enzymer
US5698504A (en) 1993-07-01 1997-12-16 The Procter & Gamble Company Machine dishwashing composition containing oxygen bleach and paraffin oil and benzotriazole compound silver tarnishing inhibitors
US5486303A (en) 1993-08-27 1996-01-23 The Procter & Gamble Company Process for making high density detergent agglomerates using an anhydrous powder additive
DE4332692C1 (de) 1993-09-25 1994-07-28 Deutsches Wollforschinst Verfahren zur Druckvorbehandlung und/oder Filzfreiausrüstung von Wolle
US5861271A (en) 1993-12-17 1999-01-19 Fowler; Timothy Cellulase enzymes and systems for their expressions
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
ES2364774T3 (es) 1994-02-24 2011-09-14 HENKEL AG & CO. KGAA Enzimas mejoradas y detergentes que las contienen.
WO1995023221A1 (en) 1994-02-24 1995-08-31 Cognis, Inc. Improved enzymes and detergents containing them
US5691295A (en) 1995-01-17 1997-11-25 Cognis Gesellschaft Fuer Biotechnologie Mbh Detergent compositions
US5686014A (en) 1994-04-07 1997-11-11 The Procter & Gamble Company Bleach compositions comprising manganese-containing bleach catalysts
PT766727E (pt) 1994-06-17 2002-11-29 Genencor Int Composicoes de limpeza contendo enzimas degradantes de paredes de celulas de plantas e seu uso em metodos de limpeza
GB2294268A (en) 1994-07-07 1996-04-24 Procter & Gamble Bleaching composition for dishwasher use
GB9416841D0 (en) 1994-08-19 1994-10-12 Finnfeeds Int Ltd An enzyme feed additive and animal feed including it
US5879584A (en) 1994-09-10 1999-03-09 The Procter & Gamble Company Process for manufacturing aqueous compositions comprising peracids
US5516448A (en) 1994-09-20 1996-05-14 The Procter & Gamble Company Process for making a high density detergent composition which includes selected recycle streams for improved agglomerate
US5489392A (en) 1994-09-20 1996-02-06 The Procter & Gamble Company Process for making a high density detergent composition in a single mixer/densifier with selected recycle streams for improved agglomerate properties
US5691297A (en) 1994-09-20 1997-11-25 The Procter & Gamble Company Process for making a high density detergent composition by controlling agglomeration within a dispersion index
US5660982A (en) 1994-10-04 1997-08-26 Tryggvason; Karl Laminin chains: diagnostic uses
JPH10506944A (ja) 1994-10-07 1998-07-07 ノボ ノルディスク アクティーゼルスカブ 獣皮又は外皮を、トリプシン作用微生物プロテアーゼを用いて酵素処理することを含んでなる、獣皮又は外皮を革に加工する方法
PT796317E (pt) 1994-12-09 2000-08-31 Procter & Gamble Composicao para lavagem automatica de loica contendo particulas de peroxidos de diacilo
US5534179A (en) 1995-02-03 1996-07-09 Procter & Gamble Detergent compositions comprising multiperacid-forming bleach activators
US5574005A (en) 1995-03-07 1996-11-12 The Procter & Gamble Company Process for producing detergent agglomerates from high active surfactant pastes having non-linear viscoelastic properties
US5569645A (en) 1995-04-24 1996-10-29 The Procter & Gamble Company Low dosage detergent composition containing optimum proportions of agglomerates and spray dried granules for improved flow properties
ATE429490T1 (de) 1995-05-05 2009-05-15 Novozymes As Protease-varianten und verbindungen
US5597936A (en) 1995-06-16 1997-01-28 The Procter & Gamble Company Method for manufacturing cobalt catalysts
TR199701633T1 (xx) 1995-06-16 1998-04-21 The Procter & Gamble Company Kobalt kataliz�r i�eren otomatik bula��k makinas� deterjan bile�ikleri.
US5565422A (en) 1995-06-23 1996-10-15 The Procter & Gamble Company Process for preparing a free-flowing particulate detergent composition having improved solubility
DE19530816A1 (de) 1995-08-23 1997-02-27 Cognis Bio Umwelt Verwendung von mutierter Subtilisin-Protease in kosmetischen Produkten
US5576282A (en) 1995-09-11 1996-11-19 The Procter & Gamble Company Color-safe bleach boosters, compositions and laundry methods employing same
DE69620003T3 (de) 1995-09-18 2006-11-30 The Procter & Gamble Company, Cincinnati Freisetzungssysteme
MA24137A1 (fr) 1996-04-16 1997-12-31 Procter & Gamble Fabrication d'agents de surface ramifies .
EP0942931A2 (en) 1996-11-21 1999-09-22 Cor Therapeutics, Inc. Modulation of interactions between myosin and integrins
CN1263759C (zh) 1997-03-07 2006-07-12 宝洁公司 制备交联桥大环化合物的改进方法
AU731577B2 (en) 1997-03-07 2001-04-05 Procter & Gamble Company, The Bleach compositions containing metal bleach catalyst, and bleach activators and/or organic percarboxylic acids
AR016969A1 (es) 1997-10-23 2001-08-01 Procter & Gamble VARIANTE DE PROTEASA, ADN, VECTOR DE EXPRESIoN, MICROORGANISMO HUESPED, COMPOSICIoN DE LIMPIEZA, ALIMENTO PARA ANIMALES Y COMPOSICIoN PARA TRATAR UN TEXTIL
US5935826A (en) 1997-10-31 1999-08-10 National Starch And Chemical Investment Holding Corporation Glucoamylase converted starch derivatives and their use as emulsifying and encapsulating agents
US6287839B1 (en) 1997-11-19 2001-09-11 Genencor International, Inc. Cellulase producing actinomycetes, cellulase produced therefrom and method of producing same
US6562612B2 (en) 1997-11-19 2003-05-13 Genencor International, Inc. Cellulase producing actinomycetes, cellulase produced therefrom and method of producing same
AU1225099A (en) 1997-11-21 1999-06-15 Novo Nordisk A/S Protease variants and compositions
AU2207099A (en) 1997-12-24 1999-07-19 Genencor International, Inc. An improved method of assaying for a preferred enzyme and/or preferred detergentcomposition
EP2261359B1 (en) 1998-06-10 2014-08-20 Novozymes A/S Mannanases
US6376450B1 (en) 1998-10-23 2002-04-23 Chanchal Kumar Ghosh Cleaning compositions containing multiply-substituted protease variants
AU2026100A (en) 1998-11-30 2000-06-19 Procter & Gamble Company, The Process for preparing cross-bridged tetraaza macrocycles
US20020142934A1 (en) * 1999-09-09 2002-10-03 Procter & Gamble Company Detergent composition containing a protease
PL203212B1 (pl) 2000-02-08 2009-09-30 Dsm Ip Assets Bv Zastosowanie trwałej w kwasie proteazy w karmie zwierzęcej oraz w wytwarzaniu kompozycji do stosowania w karmie zwierzęcej, dodatek do karmy zwierzęcej, kompozycja karmy zwierzęcej i sposób obróbki białek roślinnych
CA2418317A1 (en) 2000-08-11 2002-02-21 Genencor International, Inc. Bacillus transformation, transformants and mutant libraries
US6440991B1 (en) 2000-10-02 2002-08-27 Wyeth Ethers of 7-desmethlrapamycin
US6846653B2 (en) 2000-10-10 2005-01-25 Genencor International, Inc. Enhanced secretion of a polypeptide by a microorganism
US6582914B1 (en) 2000-10-26 2003-06-24 Genencor International, Inc. Method for generating a library of oligonucleotides comprising a controlled distribution of mutations
CA2442741A1 (en) 2001-03-27 2003-01-16 Human Genome Sciences, Inc. Human secreted proteins
BR0117078B1 (pt) 2001-07-14 2011-06-14 mÉtodo para preparaÇço de couro utilizando protease e mÉtodo para tratamento de dejetos derivados do processamento do couro utilizando o mesmo.
MXPA04006811A (es) 2002-01-16 2004-10-11 Genencor Int Variantes de proteasa substituidas con multiplos.
WO2004072279A2 (en) 2003-02-07 2004-08-26 Novozymes A/S Proteases
RU2364626C2 (ru) * 2003-03-18 2009-08-20 Ново Нордиск Хелт Кэр Аг Способ получения, способ очистки и способ стабилизации полипептидов фактора vii, ix и x
EP2311942A1 (en) 2003-03-21 2011-04-20 Genecor International, Inc. Novel cbh1 homologs and variant cbh1 cellulases
WO2005052146A2 (en) 2003-11-19 2005-06-09 Genencor International, Inc. Serine proteases, nucleic acids encoding serine enzymes and vectors and host cells incorporating same
DE102004029475A1 (de) 2004-06-18 2006-01-26 Henkel Kgaa Neues enzymatisches Bleichsystem
US7473544B2 (en) * 2004-10-08 2009-01-06 Kao Corporation Alkaline protease
US7378256B2 (en) 2004-11-18 2008-05-27 Genencor International, Inc. Aspergillus niger promoter for expressing genes in host cells
JP5785686B2 (ja) * 2006-06-23 2015-09-30 ダニスコ・ユーエス・インク、ジェネンコー・ディビジョン 複数の性質をエンジニアリングするための部位評価ライブラリーを用いた、配列と活性との関係の系統的な評価

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US8455234B2 (en) 2013-06-04
US7985569B2 (en) 2011-07-26
US20080063774A1 (en) 2008-03-13
US20150072913A1 (en) 2015-03-12
BRPI0718483A2 (pt) 2013-11-26
WO2008048392A1 (en) 2008-04-24
JP5775666B2 (ja) 2015-09-09
JP2010506590A (ja) 2010-03-04
US20110251116A1 (en) 2011-10-13
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US20140005094A1 (en) 2014-01-02
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