KR20070085922A - 니트릴히드라타제를 발현하는 형질 전환체 - Google Patents

니트릴히드라타제를 발현하는 형질 전환체 Download PDF

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KR20070085922A
KR20070085922A KR1020077012947A KR20077012947A KR20070085922A KR 20070085922 A KR20070085922 A KR 20070085922A KR 1020077012947 A KR1020077012947 A KR 1020077012947A KR 20077012947 A KR20077012947 A KR 20077012947A KR 20070085922 A KR20070085922 A KR 20070085922A
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South Korea
Prior art keywords
nitrile hydratase
subunit
sequence
dna
seq
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KR1020077012947A
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English (en)
Korean (ko)
Inventor
가오루 후루야
아끼라 다마끼
신이찌로 나가사와
아야노 스즈끼
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아사히 가세이 가부시키가이샤
동서석유화학주식회사
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Publication of KR20070085922A publication Critical patent/KR20070085922A/ko
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/02—Amides, e.g. chloramphenicol or polyamides; Imides or polyimides; Urethanes, i.e. compounds comprising N-C=O structural element or polyurethanes

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
KR1020077012947A 2004-12-09 2005-12-09 니트릴히드라타제를 발현하는 형질 전환체 Ceased KR20070085922A (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2004357035 2004-12-09
JPJP-P-2004-00357035 2004-12-09

Publications (1)

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KR20070085922A true KR20070085922A (ko) 2007-08-27

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ID=36578009

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KR1020077012947A Ceased KR20070085922A (ko) 2004-12-09 2005-12-09 니트릴히드라타제를 발현하는 형질 전환체

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JP (1) JPWO2006062189A1 (https=)
KR (1) KR20070085922A (https=)
TW (1) TW200634151A (https=)
WO (1) WO2006062189A1 (https=)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5080787B2 (ja) * 2006-11-15 2012-11-21 達彦 小林 タンパク質改変方法、ニトリルヒドラターゼ成熟化方法、成熟化ニトリルヒドラターゼ生産方法、成熟化ニトリルヒドラターゼを用いたアミド化合物生産方法
JP5817088B2 (ja) * 2010-03-24 2015-11-18 三菱レイヨン株式会社 ニトリルヒドラターゼ遺伝子を欠失又は不活性化させた微生物
JP2011217665A (ja) * 2010-04-08 2011-11-04 Mitsubishi Rayon Co Ltd ニトリルヒドラターゼ遺伝子を置換した微生物
BR112014020899A2 (pt) * 2012-02-28 2018-05-02 Mitsubishi Rayon Co., Ltd. métodos para produzir, preservar, e ativar nitrila hidratase
JP6156442B2 (ja) * 2015-05-28 2017-07-05 三菱ケミカル株式会社 ニトリルヒドラターゼ遺伝子を置換した微生物
KR102324118B1 (ko) * 2016-12-28 2021-11-08 미쓰이 가가쿠 가부시키가이샤 변이형 나이트릴 히드라타아제, 그 변이형 나이트릴 히드라타아제를 코드하는 핵산, 그 핵산을 포함하는 발현 벡터 및 형질전환체, 그 변이형 나이트릴 히드라타아제의 제조 방법, 및 아마이드 화합물의 제조 방법
JP7149337B2 (ja) * 2017-11-14 2022-10-06 コロンビア エス．アール．エル． アミド調製のための微生物プロセス
CN111334495B (zh) * 2020-03-12 2022-04-29 宜昌东阳光生化制药有限公司 制备右旋酰胺酮洛芬的方法
CN119220525B (zh) * 2024-09-23 2025-11-21 浙江工业大学 腈水解酶突变体及其在饲料中的应用

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS63137688A (ja) * 1986-12-01 1988-06-09 Res Assoc Util Of Light Oil アミド化合物の製造法
JPH0753104B2 (ja) * 1989-02-28 1995-06-07 秀明 山田 細菌の培養法
FR2708936B1 (fr) * 1993-08-10 1995-11-10 Rhone Poulenc Chimie Enzymes à activité nitrile-hydratase, outils génétiques et micro-organismes hôtes permettant leur obtention et procédé d'hydrolyse mettant en Óoeuvre lesdites enzymes.
JP3763157B2 (ja) * 1996-03-18 2006-04-05 住友化学株式会社 ニトリルヒドラターゼ、その遺伝子及びその利用
US6153415A (en) * 1998-04-29 2000-11-28 Board Of Trustees Operating Michigan State University Method for producing amide compounds using a nitrile hydratase from a thermophilic bacillus
KR100320038B1 (ko) * 1999-11-19 2002-02-01 이상현 로도코커스 로도크로스(Ｒｈｏｄｏｃｏｃｃｕｓｒｈｏｄｏｃｈｒｏｕｓ) Ｍ33 ＶＫＭ Ａｃ-1515Ｄ로부터유래된 니트릴 히드라타제를 코딩하는 유전자 및 이유전자를 함유한 형질전환체
CN1806047A (zh) * 2003-06-10 2006-07-19 旭化成株式会社 新型腈水合酶

Also Published As

Publication number Publication date
JPWO2006062189A1 (ja) 2008-06-12
TWI327596B (https=) 2010-07-21
TW200634151A (en) 2006-10-01
WO2006062189A1 (ja) 2006-06-15

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