KR20010072050A - 다공성 규소를 사용하여 물질을 세포로 이동시키는 방법 - Google Patents
다공성 규소를 사용하여 물질을 세포로 이동시키는 방법 Download PDFInfo
- Publication number
- KR20010072050A KR20010072050A KR1020017001028A KR20017001028A KR20010072050A KR 20010072050 A KR20010072050 A KR 20010072050A KR 1020017001028 A KR1020017001028 A KR 1020017001028A KR 20017001028 A KR20017001028 A KR 20017001028A KR 20010072050 A KR20010072050 A KR 20010072050A
- Authority
- KR
- South Korea
- Prior art keywords
- porous silicon
- cells
- needle
- silicon
- cell
- Prior art date
Links
- 229910021426 porous silicon Inorganic materials 0.000 title claims abstract description 102
- 239000000463 material Substances 0.000 title claims abstract description 63
- 238000000034 method Methods 0.000 claims abstract description 51
- 230000000975 bioactive effect Effects 0.000 claims abstract description 23
- 239000011148 porous material Substances 0.000 claims abstract description 19
- 229910000389 calcium phosphate Inorganic materials 0.000 claims abstract description 16
- 239000001506 calcium phosphate Substances 0.000 claims abstract description 16
- 235000011010 calcium phosphates Nutrition 0.000 claims abstract description 16
- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 claims abstract description 16
- 239000000126 substance Substances 0.000 claims description 52
- 239000012530 fluid Substances 0.000 claims description 19
- 238000012546 transfer Methods 0.000 claims description 9
- 239000002244 precipitate Substances 0.000 claims description 6
- 238000000576 coating method Methods 0.000 claims description 5
- 239000012634 fragment Substances 0.000 claims description 5
- 210000003296 saliva Anatomy 0.000 claims description 5
- RTAQQCXQSZGOHL-UHFFFAOYSA-N Titanium Chemical compound [Ti] RTAQQCXQSZGOHL-UHFFFAOYSA-N 0.000 claims description 2
- 239000010936 titanium Substances 0.000 claims description 2
- 229910052719 titanium Inorganic materials 0.000 claims description 2
- 239000011247 coating layer Substances 0.000 claims 1
- 238000001000 micrograph Methods 0.000 claims 1
- 239000002344 surface layer Substances 0.000 claims 1
- 230000000149 penetrating effect Effects 0.000 abstract description 16
- 229920001410 Microfiber Polymers 0.000 abstract description 11
- 239000003658 microfiber Substances 0.000 abstract description 11
- 239000002245 particle Substances 0.000 abstract description 6
- 230000008901 benefit Effects 0.000 abstract description 5
- 238000001556 precipitation Methods 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 description 97
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 45
- 108020004414 DNA Proteins 0.000 description 39
- 229910052710 silicon Inorganic materials 0.000 description 39
- 239000010703 silicon Substances 0.000 description 39
- 239000003981 vehicle Substances 0.000 description 24
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 16
- 229910052757 nitrogen Inorganic materials 0.000 description 8
- 239000011856 silicon-based particle Substances 0.000 description 7
- 230000008569 process Effects 0.000 description 6
- 235000012431 wafers Nutrition 0.000 description 6
- 238000003491 array Methods 0.000 description 5
- 238000004520 electroporation Methods 0.000 description 5
- 238000001727 in vivo Methods 0.000 description 5
- 238000004519 manufacturing process Methods 0.000 description 5
- 229910021420 polycrystalline silicon Inorganic materials 0.000 description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- 239000011248 coating agent Substances 0.000 description 4
- 238000005530 etching Methods 0.000 description 4
- 102000004169 proteins and genes Human genes 0.000 description 4
- 108090000623 proteins and genes Proteins 0.000 description 4
- 239000000758 substrate Substances 0.000 description 4
- 239000012620 biological material Substances 0.000 description 3
- 210000000170 cell membrane Anatomy 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 3
- 238000000975 co-precipitation Methods 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 3
- 229910052737 gold Inorganic materials 0.000 description 3
- 239000010931 gold Substances 0.000 description 3
- 210000004962 mammalian cell Anatomy 0.000 description 3
- MWUXSHHQAYIFBG-UHFFFAOYSA-N nitrogen oxide Inorganic materials O=[N] MWUXSHHQAYIFBG-UHFFFAOYSA-N 0.000 description 3
- 239000007787 solid Substances 0.000 description 3
- WFKWXMTUELFFGS-UHFFFAOYSA-N tungsten Chemical compound [W] WFKWXMTUELFFGS-UHFFFAOYSA-N 0.000 description 3
- 229910052721 tungsten Inorganic materials 0.000 description 3
- 239000010937 tungsten Substances 0.000 description 3
- 239000013598 vector Substances 0.000 description 3
- KRHYYFGTRYWZRS-UHFFFAOYSA-N Fluorane Chemical compound F KRHYYFGTRYWZRS-UHFFFAOYSA-N 0.000 description 2
- 230000001464 adherent effect Effects 0.000 description 2
- 239000012080 ambient air Substances 0.000 description 2
- 238000002048 anodisation reaction Methods 0.000 description 2
- 230000008512 biological response Effects 0.000 description 2
- 239000011575 calcium Substances 0.000 description 2
- 210000002421 cell wall Anatomy 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 238000010304 firing Methods 0.000 description 2
- 239000007789 gas Substances 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 239000001307 helium Substances 0.000 description 2
- 229910052734 helium Inorganic materials 0.000 description 2
- SWQJXJOGLNCZEY-UHFFFAOYSA-N helium atom Chemical compound [He] SWQJXJOGLNCZEY-UHFFFAOYSA-N 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 239000011159 matrix material Substances 0.000 description 2
- 238000000386 microscopy Methods 0.000 description 2
- 108020004707 nucleic acids Proteins 0.000 description 2
- 102000039446 nucleic acids Human genes 0.000 description 2
- 150000007523 nucleic acids Chemical class 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 230000035939 shock Effects 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 1
- GRYLNZFGIOXLOG-UHFFFAOYSA-N Nitric acid Chemical compound O[N+]([O-])=O GRYLNZFGIOXLOG-UHFFFAOYSA-N 0.000 description 1
- 239000011149 active material Substances 0.000 description 1
- 210000004102 animal cell Anatomy 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 229920000704 biodegradable plastic Polymers 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 108091092356 cellular DNA Proteins 0.000 description 1
- 238000001311 chemical methods and process Methods 0.000 description 1
- 230000007797 corrosion Effects 0.000 description 1
- 238000005260 corrosion Methods 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 239000008367 deionised water Substances 0.000 description 1
- 229910021641 deionized water Inorganic materials 0.000 description 1
- 230000008021 deposition Effects 0.000 description 1
- 230000001627 detrimental effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 239000000975 dye Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000003628 erosive effect Effects 0.000 description 1
- 238000010353 genetic engineering Methods 0.000 description 1
- 238000007654 immersion Methods 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 150000002500 ions Chemical group 0.000 description 1
- 230000007794 irritation Effects 0.000 description 1
- 239000002502 liposome Substances 0.000 description 1
- 238000001459 lithography Methods 0.000 description 1
- 238000010297 mechanical methods and process Methods 0.000 description 1
- 230000005226 mechanical processes and functions Effects 0.000 description 1
- 230000001404 mediated effect Effects 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 239000007769 metal material Substances 0.000 description 1
- 239000002923 metal particle Substances 0.000 description 1
- 238000004377 microelectronic Methods 0.000 description 1
- 238000013508 migration Methods 0.000 description 1
- 230000005012 migration Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 210000005036 nerve Anatomy 0.000 description 1
- 229910017604 nitric acid Inorganic materials 0.000 description 1
- 239000002773 nucleotide Substances 0.000 description 1
- 125000003729 nucleotide group Chemical group 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 230000004962 physiological condition Effects 0.000 description 1
- 238000009832 plasma treatment Methods 0.000 description 1
- 239000013612 plasmid Substances 0.000 description 1
- 230000001376 precipitating effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 229920006395 saturated elastomer Polymers 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 238000012732 spatial analysis Methods 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000001890 transfection Methods 0.000 description 1
- 238000000844 transformation Methods 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 239000011882 ultra-fine particle Substances 0.000 description 1
- 230000003612 virological effect Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0012—Galenical forms characterised by the site of application
- A61K9/0019—Injectable compositions; Intramuscular, intravenous, arterial, subcutaneous administration; Compositions to be administered through the skin in an invasive manner
- A61K9/0021—Intradermal administration, e.g. through microneedle arrays, needleless injectors
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M37/00—Other apparatus for introducing media into the body; Percutany, i.e. introducing medicines into the body by diffusion through the skin
- A61M37/0015—Other apparatus for introducing media into the body; Percutany, i.e. introducing medicines into the body by diffusion through the skin by using microneedles
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/89—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation using microinjection
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/89—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation using microinjection
- C12N15/895—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation using microinjection using biolistic methods
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M37/00—Other apparatus for introducing media into the body; Percutany, i.e. introducing medicines into the body by diffusion through the skin
- A61M37/0015—Other apparatus for introducing media into the body; Percutany, i.e. introducing medicines into the body by diffusion through the skin by using microneedles
- A61M2037/0046—Solid microneedles
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M37/00—Other apparatus for introducing media into the body; Percutany, i.e. introducing medicines into the body by diffusion through the skin
- A61M37/0015—Other apparatus for introducing media into the body; Percutany, i.e. introducing medicines into the body by diffusion through the skin by using microneedles
- A61M2037/0053—Methods for producing microneedles
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Plant Pathology (AREA)
- Dermatology (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Veterinary Medicine (AREA)
- Cell Biology (AREA)
- Pharmacology & Pharmacy (AREA)
- Anesthesiology (AREA)
- Heart & Thoracic Surgery (AREA)
- Hematology (AREA)
- Sustainable Development (AREA)
- Medicinal Chemistry (AREA)
- Medical Informatics (AREA)
- Epidemiology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Silicon Compounds (AREA)
- Electrotherapy Devices (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Battery Electrode And Active Subsutance (AREA)
- Medicinal Preparation (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Description
Claims (33)
- 다공성 규소를 사용하여 물질을 세포로 이동시킴을 포함하여, 물질을 세포로 이동시키는 방법.
- 제1항에 있어서, 재흡수성 또는 생체부식성 다공성 규소를 사용함을 포함하는 방법.
- 제1항 또는 제2항에 있어서, 적어도 부분적으로 다공성 규소를 포함하는 현미침을 사용함을 포함하는 방법.
- 제3항에 있어서, 적어도 침의 첨단(tip)에 다공성 규소가 제공됨을 포함하는 방법.
- 제1항 내지 제4항 중의 어느 한 항에 있어서, (i) 다공성 규소 피막을 갖고, (ii) 길이의 적어도 일부가 실질적으로 완전히 다공성 규소로 제조된 현미침을 사용함을 포함하는 방법.
- 제1항 내지 제5항 중의 어느 한 항에 있어서, 적어도 부분적으로 다공성 규소를 포함하는 현미침의 배열을 사용함을 포함하는 방법.
- 제1항 내지 제6항 중의 어느 한 항에 있어서, 중공(hollow)을 갖고 다공성 규소를 포함하며, 물질이 중공으로 제공되거나 중공을 통하여 이동하는 현미침 또는 현미침 배열을 사용함을 포함하는 방법.
- 제1항 내지 제7항 중의 어느 한 항에 있어서, 지지재로부터 연장되고, 실질적으로 완전히 또는 적어도 부분적으로 다공성 규소를 포함하며, 각각의 기저로부터 첨단까지 유체를 이동시키도록 개조된 유체 운송 수단을 갖는 복수의 침, 및 유체 운송 수단과 연결되어 있고 침의 기저에 주입되는 유체를 공급하는 유체 공급 수단을 포함하는 현미침 배열이 사용되는 방법.
- 제3항 내지 제8항 중의 어느 한 항에 있어서, 침이 중앙 루멘(central lumen)을 갖지 않고 마이크로피어서(micropiercer)를 포함하며, 다공성 규소가 침 위에 제공되어 운반되는 물질을 고정시키는 방법.
- 제1항 내지 제9항 중의 어느 한 항에 있어서, 저장소 또는 채널로부터 침의 표면에 제공된 물질 운반 영역에 이르는 기공 망상 조직을 갖는 침 또는 침들을 제공함을 포함하는 방법.
- 제1항 내지 제10항 중의 어느 한 항에 있어서, 다공성 규소가 벌크한 티탄과같은 생체불활성 물질 위에 제공되는 경우 물질을 이의 이동성과 비교하여 부동화시키는 방법.
- 제1항 또는 제2항에 있어서, 다공성 규소 바이오리스틱 탄환을 포함하는 방법.
- 제1항 또는 제2항에 있어서, 결합된 물질을 갖는 다공성 규소를 사용하고, 세포로 흡수되는 공침물을 형성하는 또 다른 물질과 공침되도록 개조된 형태로 다공성 규소를 제공함을 포함하는 방법.
- 제13항에 있어서, 공침물로서 인산칼슘을 포함하는 방법.
- 제1항 또는 제2항에 있어서, 적어도 부분적으로 다공성 규소를 포함하는 전기적으로 생체활성인 전극을 사용하고, 전자기공형성법(electroporosis)을 사용하여 물질을 세포로 이동시킴을 포함하는 방법.
- 제15항에 있어서, 세포가 전극에 부착되는 방법.
- 제1항 내지 제16항 중의 어느 한 항에 있어서, 물질이 DNA 또는 RNA, DNA 또는 RNA의 단편, 또는 DNA 또는 RNA의 구성물을 포함하는 방법.
- 다공성 규소를 포함하는 현미침.
- 제18항에 있어서, 도관(duct)을 갖는 현미침.
- 제19항에 있어서, 도관이 침의 기저 영역으로부터 침의 첨단에 이르는 현미침.
- 제18항 내지 제20항 중의 어느 한 항에 있어서, 침의 적어도 일부가 다공성 규소로 실질적으로 완전히 이루어지는 현미침.
- 제18항 내지 제21항 중의 어느 한 항에 있어서, 침의 적어도 일부가 다공성 규소의 피막 또는 표면 층을 포함하는 현미침.
- 제18항 내지 제22항 중의 어느 한 항에 있어서, 다공성 또는 모세관 망상 구조가 제공되는 현미침.
- 제18항 내지 제23항 중의 어느 한 항에 있어서, 세포로 운반되도록 개조된 물질을 추가로 포함하는 현미침 배열.
- 제24항에 있어서, 물질이 다공성 규소에 의해 운반되거나, 침 위에 고정되는 침.
- 제18항 내지 제25항 중의 어느 한 항에 있어서, 재흡수성 또는 생체흡수성이거나, 적어도 부분적으로 재흡수성 또는 생체흡수성인 침.
- 현미침이 제18항 내지 제26항 중의 어느 한 항에 따르는, 지지재로부터 확장된 현미침의 배열.
- 적어도 부분적으로 다공성 규소 및 세포로 이동시키는 물질을 포함하는, 물질을 세포로 이동시키기 위한 비히클.
- 제28항에 있어서, 다공성 규소가 재흡수성인 비히클.
- 제28항 또는 제29항에 있어서, 다공성 규소 바이오리스틱 탄환을 포함하는 비히클.
- 제28항 또는 제29항에 있어서, 사용시 세포로 흡수되는 공침물과 공침되는 물질을 포함하는 비히클.
- 제28항 또는 제29항에 있어서, 전기 전도성 생체활성 다공성 규소 전극을 포함하는 비히클.
- 물질을 살아있는 세포로 이동시키기 위한 이동 매질로서의 다공성 규소의 용도.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GBGB9815819.9A GB9815819D0 (en) | 1998-07-22 | 1998-07-22 | Transferring materials into cells and a microneedle array |
GB9815819.9 | 1998-07-22 |
Publications (2)
Publication Number | Publication Date |
---|---|
KR20010072050A true KR20010072050A (ko) | 2001-07-31 |
KR100633273B1 KR100633273B1 (ko) | 2006-10-16 |
Family
ID=10835869
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
KR1020017001028A KR100633273B1 (ko) | 1998-07-22 | 1999-07-22 | 다공성 규소를 사용하여 물질을 세포로 이동시키는 방법 |
Country Status (15)
Country | Link |
---|---|
US (2) | US6770480B1 (ko) |
EP (3) | EP1522578B1 (ko) |
JP (1) | JP4585119B2 (ko) |
KR (1) | KR100633273B1 (ko) |
CN (1) | CN1252275C (ko) |
AT (1) | ATE223479T1 (ko) |
AU (1) | AU753542B2 (ko) |
CA (1) | CA2337331C (ko) |
DE (1) | DE69902794T2 (ko) |
DK (1) | DK1098957T3 (ko) |
ES (2) | ES2179666T3 (ko) |
GB (1) | GB9815819D0 (ko) |
HK (1) | HK1039146B (ko) |
NZ (1) | NZ509142A (ko) |
WO (1) | WO2000005339A1 (ko) |
Families Citing this family (121)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB9808052D0 (en) | 1998-04-17 | 1998-06-17 | Secr Defence | Implants for administering substances and methods of producing implants |
GB9815819D0 (en) * | 1998-07-22 | 1998-09-16 | Secr Defence | Transferring materials into cells and a microneedle array |
GB0008494D0 (en) | 2000-04-07 | 2000-05-24 | Secr Defence | Microprojectile delivery system |
GB2363115A (en) * | 2000-06-10 | 2001-12-12 | Secr Defence | Porous or polycrystalline silicon orthopaedic implants |
US6533949B1 (en) * | 2000-08-28 | 2003-03-18 | Nanopass Ltd. | Microneedle structure and production method therefor |
US6821281B2 (en) | 2000-10-16 | 2004-11-23 | The Procter & Gamble Company | Microstructures for treating and conditioning skin |
US7828827B2 (en) | 2002-05-24 | 2010-11-09 | Corium International, Inc. | Method of exfoliation of skin using closely-packed microstructures |
US6902933B2 (en) | 2000-11-03 | 2005-06-07 | Regents Of The University Of Michigan | Surface transfection and expression procedure |
US6897067B2 (en) | 2000-11-03 | 2005-05-24 | Regents Of The University Of Michigan | Surface transfection and expression procedure |
CA2427916C (en) * | 2000-11-03 | 2012-01-17 | The Regents Of The University Of Michigan | Surface transfection and expression procedure |
DE60216735D1 (de) * | 2001-02-21 | 2007-01-25 | Univ Rochester | Mikrokavitätsbiosensor, herstellungsverfahren und verwendungen davon |
GB0104383D0 (en) | 2001-02-22 | 2001-04-11 | Psimedica Ltd | Cancer Treatment |
US6663820B2 (en) * | 2001-03-14 | 2003-12-16 | The Procter & Gamble Company | Method of manufacturing microneedle structures using soft lithography and photolithography |
WO2002088165A1 (en) * | 2001-04-30 | 2002-11-07 | Doranz Benjamin J | Elucidation of gene function |
US20040087992A1 (en) * | 2002-08-09 | 2004-05-06 | Vladimir Gartstein | Microstructures for delivering a composition cutaneously to skin using rotatable structures |
US7429258B2 (en) | 2001-10-26 | 2008-09-30 | Massachusetts Institute Of Technology | Microneedle transport device |
US6908453B2 (en) * | 2002-01-15 | 2005-06-21 | 3M Innovative Properties Company | Microneedle devices and methods of manufacture |
JP2004121064A (ja) * | 2002-10-01 | 2004-04-22 | Jfe Steel Kk | 遺伝子制御用のセラミック被覆針 |
AU2003222691A1 (en) * | 2002-04-30 | 2003-11-17 | Morteza Shirkhanzadeh | Arrays of microneedles comprising porous calcium phosphate coating and bioactive agents |
US6864692B1 (en) * | 2002-06-20 | 2005-03-08 | Xsilogy, Inc. | Sensor having improved selectivity |
CA2492867C (en) | 2002-07-19 | 2011-07-05 | 3M Innovative Properties Company | Microneedle devices and microneedle delivery apparatus |
US7244513B2 (en) * | 2003-02-21 | 2007-07-17 | Nano-Proprietary, Inc. | Stain-etched silicon powder |
US7578954B2 (en) * | 2003-02-24 | 2009-08-25 | Corium International, Inc. | Method for manufacturing microstructures having multiple microelements with through-holes |
US20070087436A1 (en) * | 2003-04-11 | 2007-04-19 | Atsushi Miyawaki | Microinjection method and device |
CA2536249A1 (en) | 2003-08-25 | 2005-03-10 | 3M Innovative Properties Company | Delivery of immune response modifier compounds |
GB0402131D0 (en) * | 2004-01-30 | 2004-03-03 | Isis Innovation | Delivery method |
EP1718452A1 (en) * | 2004-02-23 | 2006-11-08 | 3M Innovative Properties Company | Method of molding for microneedle arrays |
CA2560840C (en) | 2004-03-24 | 2014-05-06 | Corium International, Inc. | Transdermal delivery device |
US7591806B2 (en) * | 2004-05-18 | 2009-09-22 | Bai Xu | High-aspect-ratio microdevices and methods for transdermal delivery and sampling of active substances |
US20090208556A1 (en) * | 2004-10-29 | 2009-08-20 | Regents Of The University Of California, The | Porous photonic crystals for drug delivery to the eye |
DE602005021541D1 (de) | 2004-12-07 | 2010-07-08 | 3M Innovative Properties Co | Verfahren zum formen einer mikronadel |
US20060276047A1 (en) * | 2005-03-14 | 2006-12-07 | University Of Rochester | Macroporous silicon microcavity with tunable pore size |
EP1888459A4 (en) * | 2005-05-09 | 2010-12-29 | Vesta Res Ltd | SILICON NANOEPONGES PARTICLES |
US8048017B2 (en) * | 2005-05-18 | 2011-11-01 | Bai Xu | High-aspect-ratio microdevices and methods for transdermal delivery and sampling of active substances |
US8043250B2 (en) * | 2005-05-18 | 2011-10-25 | Nanomed Devices, Inc. | High-aspect-ratio microdevices and methods for transdermal delivery and sampling of active substances |
CA2612005C (en) | 2005-06-17 | 2013-10-29 | Georgia Tech Research Corporation | Coated microstructures and method of manufacture thereof |
EP1896115B2 (en) | 2005-06-27 | 2020-01-22 | 3M Innovative Properties Company | Microneedle cartridge assembly |
CN101227940A (zh) * | 2005-07-25 | 2008-07-23 | 纳米技术维多利亚有限公司 | 微阵列装置 |
US20070066934A1 (en) * | 2005-09-19 | 2007-03-22 | Transport Pharmaceuticals, Inc. | Electrokinetic delivery system and methods therefor |
US20070185432A1 (en) * | 2005-09-19 | 2007-08-09 | Transport Pharmaceuticals, Inc. | Electrokinetic system and method for delivering methotrexate |
US20080262416A1 (en) * | 2005-11-18 | 2008-10-23 | Duan Daniel C | Microneedle Arrays and Methods of Preparing Same |
CA2629193C (en) * | 2005-11-18 | 2016-03-29 | 3M Innovative Properties Company | Coatable compositions, coatings derived therefrom and microarrays having such coatings |
EP1962942A1 (en) * | 2005-12-21 | 2008-09-03 | 3M Innovative Properties Company | Microneedle devices |
WO2007076458A1 (en) * | 2005-12-21 | 2007-07-05 | Primegen Biotech Llc | Microinjector chip |
US9339641B2 (en) | 2006-01-17 | 2016-05-17 | Emkinetics, Inc. | Method and apparatus for transdermal stimulation over the palmar and plantar surfaces |
US9610459B2 (en) | 2009-07-24 | 2017-04-04 | Emkinetics, Inc. | Cooling systems and methods for conductive coils |
US20100168501A1 (en) * | 2006-10-02 | 2010-07-01 | Daniel Rogers Burnett | Method and apparatus for magnetic induction therapy |
DE102006028781A1 (de) * | 2006-06-23 | 2007-12-27 | Robert Bosch Gmbh | Verfahren zur Herstellung von porösen Mikronadeln und ihre Verwendung |
WO2008042902A2 (en) | 2006-10-02 | 2008-04-10 | Emkinetics, Inc. | Method and apparatus for magnetic induction therapy |
US9005102B2 (en) | 2006-10-02 | 2015-04-14 | Emkinetics, Inc. | Method and apparatus for electrical stimulation therapy |
US11224742B2 (en) | 2006-10-02 | 2022-01-18 | Emkinetics, Inc. | Methods and devices for performing electrical stimulation to treat various conditions |
US10786669B2 (en) | 2006-10-02 | 2020-09-29 | Emkinetics, Inc. | Method and apparatus for transdermal stimulation over the palmar and plantar surfaces |
WO2008076465A1 (en) * | 2006-12-21 | 2008-06-26 | Primegen Biotech, Llc | Microinjector chip |
US10525246B2 (en) | 2006-12-22 | 2020-01-07 | Nanomed Skincare, Inc. | Microdevice and method for transdermal delivery and sampling of active substances |
US20080214987A1 (en) * | 2006-12-22 | 2008-09-04 | Nanomed Devices, Inc. | Microdevice And Method For Transdermal Delivery And Sampling Of Active Substances |
CN100998901B (zh) * | 2007-01-12 | 2012-10-10 | 中国科学院上海微系统与信息技术研究所 | 多孔硅无痛注射微针阵列及其制备方法 |
DE102007002832A1 (de) * | 2007-01-19 | 2008-07-24 | Robert Bosch Gmbh | Verfahren zur Herstellung einer Vorrichtung umfassend eine Anordnung von Mikronadeln auf einem Träger und nach diesem Verfahren herstellbare Vorrichtung |
WO2008091602A2 (en) | 2007-01-22 | 2008-07-31 | Corium International, Inc. | Applicators for microneedle arrays |
CN101678166B (zh) * | 2007-03-02 | 2012-08-22 | 特克法马许可公司 | 模块化的给药系统 |
US20090017562A1 (en) * | 2007-03-13 | 2009-01-15 | Iowa State University Research Foundation, Inc. | Raman-active reagents |
CA2686093C (en) * | 2007-04-16 | 2018-05-08 | Corium International, Inc. | Solvent-cast microneedle arrays containing active |
AU2014200648B2 (en) * | 2007-04-16 | 2015-09-24 | Corium Pharma Solutions, Inc. | Solvent-cast microneedle arrays containing active |
CA2685544C (en) * | 2007-04-27 | 2016-08-09 | Board Of Regents Of The University Of Texas System | Porous particles and methods of making thereof |
AU2008275181B2 (en) * | 2007-07-10 | 2014-06-26 | The Regents Of The University Of California | Materials and methods for delivering compositions to selected tissues |
CN103083794B (zh) | 2007-08-14 | 2016-03-02 | 弗雷德哈钦森癌症研究中心 | 用于递送治疗药物的针阵列组件和方法 |
WO2009048607A1 (en) | 2007-10-10 | 2009-04-16 | Corium International, Inc. | Vaccine delivery via microneedle arrays |
JP5178132B2 (ja) * | 2007-10-11 | 2013-04-10 | キヤノン株式会社 | 画像処理システム並びに画像処理方法 |
US9220678B2 (en) | 2007-12-24 | 2015-12-29 | The University Of Queensland | Coating method |
EP2247527A4 (en) | 2008-02-07 | 2014-10-29 | Univ Queensland | PATCH PRODUCTION |
EP2100850A1 (en) * | 2008-03-11 | 2009-09-16 | Stichting Voor De Technische Wetenschappen | Microneedle array and a method for manufacturing microneedles |
US9387000B2 (en) | 2008-05-23 | 2016-07-12 | The University Of Queensland | Analyte detection using a needle projection patch |
EP2429627B1 (en) * | 2009-04-24 | 2017-06-14 | Corium International, Inc. | Methods for manufacturing microprojection arrays |
CN105476951A (zh) | 2009-05-04 | 2016-04-13 | 普西维达公司 | 多孔硅药物洗脱颗粒 |
AU2010313487A1 (en) | 2009-10-26 | 2012-05-24 | Emkinetics, Inc. | Method and apparatus for electromagnetic stimulation of nerve, muscle, and body tissues |
MX2012012567A (es) | 2010-04-28 | 2012-11-21 | Kimberly Clark Co | Metodo para aumentar la permeabilidad de una barrera epitelial. |
CA2797205C (en) | 2010-04-28 | 2019-04-16 | Kimberly-Clark Worldwide, Inc. | Medical devices for delivery of sirna |
JP5868953B2 (ja) | 2010-04-28 | 2016-02-24 | キンバリー クラーク ワールドワイド インコーポレイテッド | 射出成形型マイクロニードルアレイ及びその製造方法 |
JP5860032B2 (ja) | 2010-04-28 | 2016-02-16 | キンバリー クラーク ワールドワイド インコーポレイテッド | 関節リウマチ薬の送達のためのデバイス |
JP6327852B2 (ja) | 2010-05-04 | 2018-05-23 | コリウム インターナショナル, インコーポレイテッド | 微小突起アレイを使用した副甲状腺ホルモンの経皮送達のための方法及びデバイス |
US8588884B2 (en) | 2010-05-28 | 2013-11-19 | Emkinetics, Inc. | Microneedle electrode |
WO2012006677A1 (en) | 2010-07-14 | 2012-01-19 | The University Of Queensland | Patch applying apparatus |
WO2012061377A1 (en) * | 2010-11-01 | 2012-05-10 | Psivida Us, Inc. | Bioerodible silicon-based devices for delivery of therapeutic agents |
US9394369B2 (en) | 2011-01-03 | 2016-07-19 | The Regents Of The University Of California | Luminescent porous silicon nanoparticles for targeted delivery and immunization |
WO2012159058A2 (en) * | 2011-05-18 | 2012-11-22 | Brigham Young University | Apparatuses and related methods for delivering biological material into a cell |
GB2492167C (en) | 2011-06-24 | 2018-12-05 | Nexeon Ltd | Structured particles |
AU2012323782B2 (en) | 2011-10-12 | 2017-04-06 | Vaxxas Pty Limited | Delivery device |
EP3542851B1 (en) | 2011-10-27 | 2021-12-15 | Sorrento Therapeutics, Inc. | Implantable devices for delivery of bioactive agents |
US20170246439A9 (en) | 2011-10-27 | 2017-08-31 | Kimberly-Clark Worldwide, Inc. | Increased Bioavailability of Transdermally Delivered Agents |
KR102145638B1 (ko) | 2011-10-27 | 2020-08-18 | 소렌토 쎄라퓨틱스, 인코포레이티드 | 고점도 생체활성 제제의 경피 전달 방법 |
CA2851828A1 (en) | 2011-10-28 | 2013-05-02 | Presage Biosciences, Inc. | Methods for drug delivery |
CN102553066B (zh) * | 2011-12-28 | 2013-07-17 | 上海交通大学 | 基于多孔微针结构的经皮给药系统 |
KR20140128379A (ko) | 2012-01-30 | 2014-11-05 | 넥세온 엘티디 | 에스아이/씨 전기활성 물질의 조성물 |
GB2499984B (en) | 2012-02-28 | 2014-08-06 | Nexeon Ltd | Composite particles comprising a removable filler |
GB2502625B (en) | 2012-06-06 | 2015-07-29 | Nexeon Ltd | Method of forming silicon |
WO2014053081A1 (en) | 2012-10-01 | 2014-04-10 | The Hong Kong University Of Science And Technology | Design and manufacture of nonelectronic, active-infusion patch and device for transdermal delivery across skin |
GB2507535B (en) | 2012-11-02 | 2015-07-15 | Nexeon Ltd | Multilayer electrode |
AU2013364053B2 (en) | 2012-12-21 | 2018-08-30 | Corium Pharma Solutions, Inc. | Microarray for delivery of therapeutic agent and methods of use |
CN105025974A (zh) * | 2013-03-12 | 2015-11-04 | 普西维达公司 | 包含硅基载体粒子的药物递送装置 |
RU2674083C2 (ru) | 2013-03-12 | 2018-12-04 | Кориум Интернэшнл, Инк. | Аппликаторы с микровыступами |
US10384046B2 (en) | 2013-03-15 | 2019-08-20 | Corium, Inc. | Microarray for delivery of therapeutic agent and methods of use |
CA2903459C (en) | 2013-03-15 | 2024-02-20 | Corium International, Inc. | Multiple impact microprojection applicators and methods of use |
WO2014150293A1 (en) | 2013-03-15 | 2014-09-25 | Corium International, Inc. | Microarray with polymer-free microstructures, methods of making, and methods of use |
WO2014151381A1 (en) | 2013-03-15 | 2014-09-25 | Psivida Us, Inc. | Bioerodible silicon-based compositions for delivery of therapeutic agents |
ES2761580T3 (es) | 2013-03-15 | 2020-05-20 | Corium Inc | Micromatrices para suministro de agente terapéutico, métodos de uso y métodos de fabricación |
KR101567203B1 (ko) | 2014-04-09 | 2015-11-09 | (주)오렌지파워 | 이차 전지용 음극 활물질 및 이의 방법 |
KR101604352B1 (ko) | 2014-04-22 | 2016-03-18 | (주)오렌지파워 | 음극 활물질 및 이를 포함하는 리튬 이차 전지 |
EP3188714A1 (en) | 2014-09-04 | 2017-07-12 | Corium International, Inc. | Microstructure array, methods of making, and methods of use |
KR101549086B1 (ko) * | 2014-11-10 | 2015-09-02 | 주식회사 스몰랩 | 마이크로 니들 및 마이크로 니들 패치 |
GB2533161C (en) | 2014-12-12 | 2019-07-24 | Nexeon Ltd | Electrodes for metal-ion batteries |
US11147954B2 (en) | 2015-02-02 | 2021-10-19 | Vaxxas Pty Limited | Microprojection array applicator and method |
US10857093B2 (en) | 2015-06-29 | 2020-12-08 | Corium, Inc. | Microarray for delivery of therapeutic agent, methods of use, and methods of making |
JP6894423B2 (ja) | 2015-07-09 | 2021-06-30 | ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニアThe Regents Of The University Of California | 融合性リポソーム被覆多孔質ケイ素ナノ粒子 |
WO2017045031A1 (en) | 2015-09-18 | 2017-03-23 | Vaxxas Pty Limited | Microprojection arrays with microprojections having large surface area profiles |
JP2017176652A (ja) * | 2016-03-31 | 2017-10-05 | 花王株式会社 | 微細中空突起具 |
KR102413416B1 (ko) | 2016-06-14 | 2022-06-27 | 넥세온 엘티디 | 금속-이온 전지들용 전극들 |
KR101746024B1 (ko) * | 2016-07-14 | 2017-06-12 | 경북대학교 산학협력단 | 다공성 코팅층이 코팅된 마이크로니들과 이것의 제조 방법 그리고 마이크로니들이 구비된 마이크로니들패치 |
WO2018176102A1 (en) | 2017-03-31 | 2018-10-04 | Vaxxas Pty Limited | Device and method for coating surfaces |
AU2018285954A1 (en) | 2017-06-13 | 2019-12-19 | Vaxxas Pty Limited | Quality control of substrate coatings |
AU2018309562A1 (en) | 2017-08-04 | 2020-02-20 | Vaxxas Pty Limited | Compact high mechanical energy storage and low trigger force actuator for the delivery of microprojection array patches (MAP) |
CN109529186B (zh) * | 2018-12-17 | 2021-05-11 | 广东工业大学 | 一种药物涂层非晶合金微针及其制作方法 |
CN110251824B (zh) * | 2019-04-30 | 2021-03-16 | 山东大学 | 一种用于透皮给药的柔性微针贴片及其制作方法 |
Family Cites Families (18)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4784737A (en) * | 1986-04-18 | 1988-11-15 | The United States Department Of Energy | Electromicroinjection of particles into living cells |
US5262128A (en) * | 1989-10-23 | 1993-11-16 | The United States Of America As Represented By The Department Of Health And Human Services | Array-type multiple cell injector |
GB8927709D0 (en) * | 1989-12-07 | 1990-02-07 | Secretary Of The State For Def | Silicon quantum wires |
WO1992001802A1 (en) * | 1990-07-19 | 1992-02-06 | Chris Brinegar | Methods and apparatus for ice-medicated introduction of substances into biological material in a non-lethal manner |
JPH0634361A (ja) * | 1992-07-15 | 1994-02-08 | Canon Inc | マイクロティップ及びこれを用いた原子間力顕微鏡装置 |
US5635216A (en) * | 1993-12-16 | 1997-06-03 | Eli Lilly And Company | Microparticle compositions containing peptides, and methods for the preparation thereof |
EP0887867B1 (en) * | 1993-11-02 | 2004-04-21 | Matsushita Electric Industrial Co., Ltd | Semiconductor device comprising an aggregate of semiconductor micro-needles |
US5457041A (en) * | 1994-03-25 | 1995-10-10 | Science Applications International Corporation | Needle array and method of introducing biological substances into living cells using the needle array |
US5591139A (en) * | 1994-06-06 | 1997-01-07 | The Regents Of The University Of California | IC-processed microneedles |
US5484720A (en) * | 1994-09-08 | 1996-01-16 | Genentech, Inc. | Methods for calcium phosphate transfection |
CA2201315A1 (en) | 1994-09-30 | 1996-04-11 | Peter Ping Ling | Direct introduction of foreign materials into cells |
JP2697645B2 (ja) * | 1994-10-31 | 1998-01-14 | 日本電気株式会社 | 半導体装置の製造方法 |
DE19525607A1 (de) * | 1995-07-14 | 1997-01-16 | Boehringer Ingelheim Kg | Transcorneales Arzneimittelfreigabesystem |
GB9611437D0 (en) * | 1995-08-03 | 1996-08-07 | Secr Defence | Biomaterial |
US6284345B1 (en) * | 1997-12-08 | 2001-09-04 | Washington University | Designer particles of micron and submicron dimension |
US6503231B1 (en) * | 1998-06-10 | 2003-01-07 | Georgia Tech Research Corporation | Microneedle device for transport of molecules across tissue |
GB9815819D0 (en) * | 1998-07-22 | 1998-09-16 | Secr Defence | Transferring materials into cells and a microneedle array |
GB0008494D0 (en) * | 2000-04-07 | 2000-05-24 | Secr Defence | Microprojectile delivery system |
-
1998
- 1998-07-22 GB GBGB9815819.9A patent/GB9815819D0/en not_active Ceased
-
1999
- 1999-07-22 JP JP2000561286A patent/JP4585119B2/ja not_active Expired - Lifetime
- 1999-07-22 CA CA2337331A patent/CA2337331C/en not_active Expired - Lifetime
- 1999-07-22 CN CNB99809028XA patent/CN1252275C/zh not_active Expired - Lifetime
- 1999-07-22 US US09/743,447 patent/US6770480B1/en not_active Expired - Lifetime
- 1999-07-22 AU AU50554/99A patent/AU753542B2/en not_active Expired
- 1999-07-22 KR KR1020017001028A patent/KR100633273B1/ko not_active IP Right Cessation
- 1999-07-22 AT AT99934933T patent/ATE223479T1/de active
- 1999-07-22 EP EP04030257A patent/EP1522578B1/en not_active Expired - Lifetime
- 1999-07-22 DK DK99934933T patent/DK1098957T3/da active
- 1999-07-22 WO PCT/GB1999/002383 patent/WO2000005339A1/en active IP Right Grant
- 1999-07-22 NZ NZ509142A patent/NZ509142A/en not_active IP Right Cessation
- 1999-07-22 ES ES99934933T patent/ES2179666T3/es not_active Expired - Lifetime
- 1999-07-22 ES ES04030257T patent/ES2400472T3/es not_active Expired - Lifetime
- 1999-07-22 EP EP99934933A patent/EP1098957B1/en not_active Expired - Lifetime
- 1999-07-22 EP EP02004895A patent/EP1231259A3/en not_active Withdrawn
- 1999-07-22 DE DE69902794T patent/DE69902794T2/de not_active Expired - Lifetime
-
2002
- 2002-01-30 HK HK02100735.8A patent/HK1039146B/zh not_active IP Right Cessation
-
2004
- 2004-05-27 US US10/854,255 patent/US7332339B2/en not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
DE69902794D1 (de) | 2002-10-10 |
ATE223479T1 (de) | 2002-09-15 |
JP2002521024A (ja) | 2002-07-16 |
US6770480B1 (en) | 2004-08-03 |
ES2400472T3 (es) | 2013-04-10 |
EP1231259A3 (en) | 2003-11-19 |
NZ509142A (en) | 2004-01-30 |
WO2000005339A1 (en) | 2000-02-03 |
AU5055499A (en) | 2000-02-14 |
EP1522578B1 (en) | 2013-01-09 |
EP1522578A1 (en) | 2005-04-13 |
HK1039146A1 (en) | 2002-04-12 |
KR100633273B1 (ko) | 2006-10-16 |
DE69902794T2 (de) | 2003-08-07 |
CN1252275C (zh) | 2006-04-19 |
EP1231259A2 (en) | 2002-08-14 |
JP4585119B2 (ja) | 2010-11-24 |
HK1039146B (zh) | 2006-10-27 |
US7332339B2 (en) | 2008-02-19 |
EP1098957A1 (en) | 2001-05-16 |
EP1098957B1 (en) | 2002-09-04 |
ES2179666T3 (es) | 2003-01-16 |
GB9815819D0 (en) | 1998-09-16 |
US20040220535A1 (en) | 2004-11-04 |
AU753542B2 (en) | 2002-10-24 |
CA2337331A1 (en) | 2000-02-03 |
CA2337331C (en) | 2012-08-28 |
DK1098957T3 (da) | 2003-01-06 |
CN1310755A (zh) | 2001-08-29 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
KR100633273B1 (ko) | 다공성 규소를 사용하여 물질을 세포로 이동시키는 방법 | |
US7358098B2 (en) | Device for capturing beads and method and apparatus for arraying beads | |
JP4530991B2 (ja) | マイクロインジェクション方法および装置 | |
CN103221091B (zh) | 用纳米线的分子递送 | |
US9316615B2 (en) | Device for trapping biologically-relevant substances and system for collecting biologically-relevant substances | |
US20150362459A1 (en) | Method and System for Concentrating Particles from a Solution | |
US7521224B2 (en) | Microelectronic cell electroporation array | |
WO2008051169A1 (en) | Tip electrode chamber for small volume electroporation | |
AU2003200223B2 (en) | Transferring materials into cells using resorbable silicon | |
US20200347393A1 (en) | Micro- and nanoneedles for plant and other cell penetration | |
EP4289929A1 (en) | Membrane structure for stimulating and sensing biological materials, and cell culture plate and microfluidic device both using the same | |
KR20200043369A (ko) | 나노 니들 및 관련된 기기 및 방법 | |
JP2006187261A (ja) | 細胞内への導入目的物質導入方法および装置 | |
Paik et al. | A highly dense nanoneedle array for intracellular gene delivery | |
WO2004033614A1 (ja) | 分子放出装置及び分子放出方法 | |
CN114874909A (zh) | 一种细胞培养装置及方法 | |
TWI274075B (en) | An apparatus of electroporation utilizing micro-electrophoresis and the method for making the same | |
US20120295345A1 (en) | Apparatuses and related methods for delivering biological material into a cell | |
Kitazawa et al. | ELECTROOSMOSIS-DRIVEN POSITIONING OF HETEROTYPIC CELLS IN OPEN-ACCESS FLOW CHAMBER | |
Goryu et al. | Electrical catching and transfer of nanoparticles via nanotip silicon probe arrays |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
N231 | Notification of change of applicant | ||
N231 | Notification of change of applicant | ||
A201 | Request for examination | ||
E902 | Notification of reason for refusal | ||
E701 | Decision to grant or registration of patent right | ||
GRNT | Written decision to grant | ||
FPAY | Annual fee payment |
Payment date: 20120924 Year of fee payment: 7 |
|
FPAY | Annual fee payment |
Payment date: 20130926 Year of fee payment: 8 |
|
FPAY | Annual fee payment |
Payment date: 20140923 Year of fee payment: 9 |
|
FPAY | Annual fee payment |
Payment date: 20150918 Year of fee payment: 10 |
|
FPAY | Annual fee payment |
Payment date: 20160921 Year of fee payment: 11 |
|
FPAY | Annual fee payment |
Payment date: 20170919 Year of fee payment: 12 |
|
FPAY | Annual fee payment |
Payment date: 20180918 Year of fee payment: 13 |
|
EXPY | Expiration of term |