KR102741603B1 - 암호화 핵산을 사용한 거대분자 분석 - Google Patents

암호화 핵산을 사용한 거대분자 분석 Download PDF

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KR102741603B1
KR102741603B1 KR1020237010494A KR20237010494A KR102741603B1 KR 102741603 B1 KR102741603 B1 KR 102741603B1 KR 1020237010494 A KR1020237010494 A KR 1020237010494A KR 20237010494 A KR20237010494 A KR 20237010494A KR 102741603 B1 KR102741603 B1 KR 102741603B1
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마크 치
케빈 군데르손
마이클 필립 웨이너
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엔코디아, 인코포레이티드
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    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6818—Sequencing of polypeptides
    • G01N33/6824—Sequencing of polypeptides involving N-terminal degradation, e.g. Edman degradation
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    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
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    • C12N9/6405—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from non-mammals not being snakes
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    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
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    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
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    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6842—Proteomic analysis of subsets of protein mixtures with reduced complexity, e.g. membrane proteins, phosphoproteins, organelle proteins
    • G—PHYSICS
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    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6845—Methods of identifying protein-protein interactions in protein mixtures
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    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
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  • Urology & Nephrology (AREA)
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  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Information Retrieval, Db Structures And Fs Structures Therefor (AREA)
  • Peptides Or Proteins (AREA)
KR1020237010494A 2016-05-02 2017-05-02 암호화 핵산을 사용한 거대분자 분석 Active KR102741603B1 (ko)

Applications Claiming Priority (8)

Application Number Priority Date Filing Date Title
US201662330841P 2016-05-02 2016-05-02
US62/330,841 2016-05-02
US201662339071P 2016-05-19 2016-05-19
US62/339,071 2016-05-19
US201662376886P 2016-08-18 2016-08-18
US62/376,886 2016-08-18
KR1020227009243A KR102516168B1 (ko) 2016-05-02 2017-05-02 암호화 핵산을 사용한 거대분자 분석
PCT/US2017/030702 WO2017192633A1 (en) 2016-05-02 2017-05-02 Macromolecule analysis employing nucleic acid encoding

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KR102741603B1 true KR102741603B1 (ko) 2024-12-16

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KR1020227009243A Active KR102516168B1 (ko) 2016-05-02 2017-05-02 암호화 핵산을 사용한 거대분자 분석
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KR1020187034711A Active KR102379048B1 (ko) 2016-05-02 2017-05-02 암호화 핵산을 사용한 거대분자 분석

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US (10) US12123878B2 (enExample)
EP (2) EP3452591B1 (enExample)
JP (5) JP7120630B2 (enExample)
KR (3) KR102741603B1 (enExample)
CN (3) CN119574874A (enExample)
AU (1) AU2017259794B2 (enExample)
CA (1) CA3022863A1 (enExample)
DK (1) DK3452591T5 (enExample)
MX (1) MX2018013341A (enExample)
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Families Citing this family (173)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20190300945A1 (en) 2010-04-05 2019-10-03 Prognosys Biosciences, Inc. Spatially Encoded Biological Assays
US11435358B2 (en) 2011-06-23 2022-09-06 Board Of Regents, The University Of Texas System Single molecule peptide sequencing
US9625469B2 (en) 2011-06-23 2017-04-18 Board Of Regents, The University Of Texas System Identifying peptides at the single molecule level
EP3511423B2 (en) 2012-10-17 2024-05-29 Spatial Transcriptomics AB Methods and product for optimising localised or spatial detection of gene expression in a tissue sample
US10545153B2 (en) 2014-09-15 2020-01-28 Board Of Regents, The University Of Texas System Single molecule peptide sequencing
EP3244992B1 (en) 2015-01-12 2023-03-08 10X Genomics, Inc. Processes for barcoding nucleic acids
SG11201707515SA (en) 2015-04-10 2017-10-30 Spatial Transcriptomics Ab Spatially distinguished, multiplex nucleic acid analysis of biological specimens
WO2016210348A2 (en) 2015-06-26 2016-12-29 Ancera, Inc. Background defocusing and clearing in ferrofluid-based capture assays
MX2018013341A (es) * 2016-05-02 2019-09-18 Encodia Inc Analisis de macromoleculas que emplea la codificacion de acido nucleico.
US10202641B2 (en) 2016-05-31 2019-02-12 Cellular Research, Inc. Error correction in amplification of samples
CN109790574A (zh) * 2016-07-22 2019-05-21 哈佛学院院长及董事 用于蛋白质鉴别的方法和组合物
SG11201901733PA (en) * 2016-09-26 2019-04-29 Cellular Res Inc Measurement of protein expression using reagents with barcoded oligonucleotide sequences
AU2017363146B2 (en) 2016-11-16 2023-11-02 Catalog Technologies, Inc. Systems for nucleic acid-based data storage
US10650312B2 (en) 2016-11-16 2020-05-12 Catalog Technologies, Inc. Nucleic acid-based data storage
DK3548652T3 (da) 2016-12-01 2024-07-08 Nautilus Subsidiary Inc Fremgangsmåder til proteinanalyse
CN110300806B (zh) * 2016-12-16 2024-03-19 艾瑞特姆有限责任公司 使用连接扩增的分子检测
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10815525B2 (en) * 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
US12264411B2 (en) 2017-01-30 2025-04-01 10X Genomics, Inc. Methods and systems for analysis
CN110214186B (zh) 2017-01-30 2023-11-24 10X基因组学有限公司 用于基于微滴的单细胞条形编码的方法和系统
GB201707140D0 (en) * 2017-05-04 2017-06-21 Oxford Nanopore Tech Ltd Method
CA3059559A1 (en) 2017-06-05 2018-12-13 Becton, Dickinson And Company Sample indexing for single cells
GB201715684D0 (en) 2017-09-28 2017-11-15 Univ Gent Means and methods for single molecule peptide sequencing
WO2019084058A2 (en) * 2017-10-23 2019-05-02 Massachusetts Institute Of Technology FUNCTIONALIZED SOLID SUPPORT
EP3704249B1 (en) 2017-10-31 2025-05-21 Encodia, Inc. Kits for analysis using nucleic acid encoding and/or label
SG11202003923PA (en) 2017-10-31 2020-05-28 Encodia Inc Methods and kits using nucleic acid encoding and/or label
WO2019104155A2 (en) * 2017-11-22 2019-05-31 The University Of Chicago Chemical probe-dependent evaluation of protein activity and uses thereof
MX2020006803A (es) 2017-12-29 2020-10-28 Nautilus Biotechnology Inc Estrategias de descodificacion para identificacion de proteinas.
WO2019157529A1 (en) 2018-02-12 2019-08-15 10X Genomics, Inc. Methods characterizing multiple analytes from individual cells or cell populations
JP7364604B2 (ja) 2018-03-16 2023-10-18 カタログ テクノロジーズ， インコーポレイテッド 核酸ベースのデータ記憶のための化学的方法
JP7326778B2 (ja) * 2018-03-19 2023-08-16 株式会社リコー 核酸試料含有容器、核酸試料含有容器の製造方法、核酸試料含有容器の製造装置及び核酸試料含有容器の製造プログラム、並びに、核酸試料
JP7407128B2 (ja) 2018-05-03 2023-12-28 ベクトン・ディキンソン・アンド・カンパニー ハイスループットマルチオミクスサンプル解析
AU2019301750A1 (en) * 2018-07-12 2021-01-28 Board Of Regents, The University Of Texas System Molecular neighborhood detection by oligonucleotides
CN108866046A (zh) * 2018-07-25 2018-11-23 辽宁润基生物科技有限公司 一种分离、纯化样品中外泌体和/或游离核酸的方法
US20200032335A1 (en) 2018-07-27 2020-01-30 10X Genomics, Inc. Systems and methods for metabolome analysis
JP7576536B2 (ja) 2018-08-03 2024-10-31 カタログ テクノロジーズ， インコーポレイテッド 誤り保護とともに核酸に基づくデータを記憶および読むためのシステムおよび方法
US11519033B2 (en) 2018-08-28 2022-12-06 10X Genomics, Inc. Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample
WO2020051162A1 (en) * 2018-09-04 2020-03-12 Encodia, Inc. Proximity interaction analysis
WO2020061036A1 (en) * 2018-09-17 2020-03-26 California Institute Of Technology Detection of molecular associations
US11078480B2 (en) * 2018-09-19 2021-08-03 California Institute Of Technology High-throughput identification of intermolecular interactions
CA3117476A1 (en) 2018-10-05 2020-04-09 Board Of Regents, The University Of Texas System Solid-phase n-terminal peptide capture and release
US20210340192A1 (en) * 2018-10-05 2021-11-04 University Of Washington Reporter constructs for nanopore-based detection of biological activity
AU2019380606A1 (en) 2018-11-15 2021-05-27 Quantum-Si Incorporated Methods and compositions for protein sequencing
WO2020123316A2 (en) * 2018-12-10 2020-06-18 10X Genomics, Inc. Methods for determining a location of a biological analyte in a biological sample
US11459607B1 (en) 2018-12-10 2022-10-04 10X Genomics, Inc. Systems and methods for processing-nucleic acid molecules from a single cell using sequential co-partitioning and composite barcodes
US11926867B2 (en) 2019-01-06 2024-03-12 10X Genomics, Inc. Generating capture probes for spatial analysis
US11649485B2 (en) 2019-01-06 2023-05-16 10X Genomics, Inc. Generating capture probes for spatial analysis
US11499979B2 (en) * 2019-01-08 2022-11-15 Massachusetts Institute Of Technology Single-molecule protein and peptide sequencing
AU2020210618A1 (en) * 2019-01-21 2021-08-12 Encodia, Inc. Methods and compositions of accelerating reactions for polypeptide analysis and related uses
WO2020163789A1 (en) * 2019-02-08 2020-08-13 The Trustees Of Columbia University In The City Of New York Single cell/exosome/vesicle protein profiling
EP3924506A1 (en) 2019-02-14 2021-12-22 Becton Dickinson and Company Hybrid targeted and whole transcriptome amplification
US11427814B2 (en) 2019-03-26 2022-08-30 Encodia, Inc. Modified cleavases, uses thereof and related kits
CN113993997A (zh) * 2019-03-26 2022-01-28 Encodia 公司 修饰的切割酶，及其使用和相关试剂盒
AU2020261334A1 (en) 2019-04-23 2021-11-18 Encodia, Inc. Methods for spatial analysis of proteins and related kits
CN114072499B (zh) * 2019-04-30 2024-08-06 Encodia公司 用于制备分析物的方法和相关试剂盒
WO2020223133A1 (en) * 2019-04-30 2020-11-05 Encodia, Inc. Methods and reagents for cleavage of the n-terminal amino acid from a polypeptide
US11610651B2 (en) 2019-05-09 2023-03-21 Catalog Technologies, Inc. Data structures and operations for searching, computing, and indexing in DNA-based data storage
JP7578294B2 (ja) * 2019-05-20 2024-11-06 エンコディア， インコーポレイテッド 空間分析のための方法および関連キット
WO2020243579A1 (en) 2019-05-30 2020-12-03 10X Genomics, Inc. Methods of detecting spatial heterogeneity of a biological sample
WO2020254672A1 (en) * 2019-06-19 2020-12-24 Therycell Gmbh Spatial characterisation of target structures in a sample
US11346842B2 (en) 2019-06-20 2022-05-31 Massachusetts Institute Of Technology Single molecule peptide sequencing methods
KR20220038367A (ko) * 2019-07-08 2022-03-28 루드비히 인스티튜트 포 캔서 리서치 리미티드 비술파이트-무함유 전체 게놈 메틸화 분석
CN110689929B (zh) * 2019-08-29 2021-12-17 浙江工业大学 一种基于接触概率辅助的蛋白质atp对接方法
US11535842B2 (en) 2019-10-11 2022-12-27 Catalog Technologies, Inc. Nucleic acid security and authentication
CN114981448A (zh) * 2019-10-28 2022-08-30 宽腾矽公司 单细胞蛋白质和核酸测序的方法
WO2021086908A1 (en) * 2019-10-28 2021-05-06 Quantum-Si Incorporated Methods, kits and devices of preparing samples for multiplex polypeptide sequencing
WO2021092433A2 (en) 2019-11-08 2021-05-14 10X Genomics, Inc. Enhancing specificity of analyte binding
CN115038794A (zh) 2019-12-23 2022-09-09 10X基因组学有限公司 在基于分区的测定中使用固定生物样品的组合物和方法
CN121022991A (zh) 2019-12-23 2025-11-28 10X基因组学有限公司 使用rna模板化连接进行空间分析的方法
US20230056532A1 (en) * 2020-01-07 2023-02-23 Encodia, Inc. Methods for information transfer and related kits
EP4073263A4 (en) * 2020-01-07 2023-11-08 Encodia, Inc. METHOD FOR STABLE COMPLEX FORMATION AND CORRESPONDING KITS
WO2021141924A1 (en) 2020-01-07 2021-07-15 Encodia, Inc. Methods for stable complex formation and related kits
US12365942B2 (en) 2020-01-13 2025-07-22 10X Genomics, Inc. Methods of decreasing background on a spatial array
US12405264B2 (en) 2020-01-17 2025-09-02 10X Genomics, Inc. Electrophoretic system and method for analyte capture
US11702693B2 (en) 2020-01-21 2023-07-18 10X Genomics, Inc. Methods for printing cells and generating arrays of barcoded cells
US11732299B2 (en) 2020-01-21 2023-08-22 10X Genomics, Inc. Spatial assays with perturbed cells
US20210230681A1 (en) 2020-01-24 2021-07-29 10X Genomics, Inc. Methods for spatial analysis using proximity ligation
EP4097228B1 (en) 2020-01-29 2024-08-14 Becton, Dickinson and Company Barcoded wells for spatial mapping of single cells through sequencing
US12076701B2 (en) 2020-01-31 2024-09-03 10X Genomics, Inc. Capturing oligonucleotides in spatial transcriptomics
US12110541B2 (en) 2020-02-03 2024-10-08 10X Genomics, Inc. Methods for preparing high-resolution spatial arrays
US11898205B2 (en) 2020-02-03 2024-02-13 10X Genomics, Inc. Increasing capture efficiency of spatial assays
US11732300B2 (en) 2020-02-05 2023-08-22 10X Genomics, Inc. Increasing efficiency of spatial analysis in a biological sample
WO2021158925A1 (en) 2020-02-07 2021-08-12 10X Genomics, Inc. Quantitative and automated permeabilization performance evaluation for spatial transcriptomics
US12281357B1 (en) 2020-02-14 2025-04-22 10X Genomics, Inc. In situ spatial barcoding
AU2021224760A1 (en) * 2020-02-21 2022-09-15 10X Genomics, Inc. Capturing genetic targets using a hybridization approach
US11891654B2 (en) 2020-02-24 2024-02-06 10X Genomics, Inc. Methods of making gene expression libraries
CN115151810A (zh) 2020-02-25 2022-10-04 贝克顿迪金森公司 实现使用单细胞样品作为单色补偿对照的双特异性探针
KR102376443B1 (ko) * 2020-02-26 2022-03-17 포항공과대학교 산학협력단 나노구조체, 나노구조체를 포함하는 바이오센서, 및 스크리닝 방법
KR102482668B1 (ko) * 2020-03-10 2022-12-29 사회복지법인 삼성생명공익재단 고유 분자 식별자의 표지 정확도를 증진하는 방법
EP4127157A4 (en) * 2020-03-24 2024-04-24 Encodia, Inc. MODIFIED DIPEPTIDE CLIVASES, CORRESPONDING USES AND CORRESPONDING KITS
CN112280829B (zh) * 2020-04-07 2023-12-26 南方科技大学 一种试剂盒、样本标记方法、单细胞测序方法
US20230193355A1 (en) * 2020-04-16 2023-06-22 Singleron (Nanjing) Biotechnologies, Ltd. Methods and compositions for high-throughput target sequencing in single cells
ES2965354T3 (es) 2020-04-22 2024-04-12 10X Genomics Inc Métodos para análisis espacial que usan eliminación de ARN elegido como diana
KR20220159496A (ko) * 2020-04-22 2022-12-02 퀀텀-에스아이 인코포레이티드 시퀀싱을 위한 장치 및 방법
AU2021271639A1 (en) 2020-05-11 2022-12-08 Catalog Technologies, Inc. Programs and functions in DNA-based data storage
EP4150118A1 (en) 2020-05-14 2023-03-22 Becton Dickinson and Company Primers for immune repertoire profiling
JP2023527764A (ja) 2020-05-20 2023-06-30 クアンタム－エスアイ インコーポレイテッド タンパク質シーケンスのための方法及び組成物
ES2989052T3 (es) 2020-05-22 2024-11-25 10X Genomics Inc Medición espacio-temporal simultánea de la expresión génica y la actividad celular
WO2021237087A1 (en) 2020-05-22 2021-11-25 10X Genomics, Inc. Spatial analysis to detect sequence variants
WO2021242834A1 (en) 2020-05-26 2021-12-02 10X Genomics, Inc. Method for resetting an array
WO2021247543A2 (en) * 2020-06-02 2021-12-09 10X Genomics, Inc. Nucleic acid library methods
CN115803445A (zh) 2020-06-02 2023-03-14 贝克顿迪金森公司 用于5撇基因表达测定的寡核苷酸和珠
US12031177B1 (en) 2020-06-04 2024-07-09 10X Genomics, Inc. Methods of enhancing spatial resolution of transcripts
ES2981265T3 (es) 2020-06-08 2024-10-08 10X Genomics Inc Métodos para determinar un margen quirúrgico y métodos de uso del mismo
WO2021252591A1 (en) 2020-06-10 2021-12-16 10X Genomics, Inc. Methods for determining a location of an analyte in a biological sample
WO2021252800A1 (en) 2020-06-11 2021-12-16 Nautilus Biotechnology, Inc. Methods and systems for computational decoding of biological, chemical, and physical entities
EP4172362B1 (en) 2020-06-25 2024-09-18 10X Genomics, Inc. Spatial analysis of dna methylation
US11761038B1 (en) 2020-07-06 2023-09-19 10X Genomics, Inc. Methods for identifying a location of an RNA in a biological sample
US11981960B1 (en) 2020-07-06 2024-05-14 10X Genomics, Inc. Spatial analysis utilizing degradable hydrogels
US12209280B1 (en) 2020-07-06 2025-01-28 10X Genomics, Inc. Methods of identifying abundance and location of an analyte in a biological sample using second strand synthesis
CA3179280A1 (en) * 2020-07-14 2022-01-20 Arjun Ganesan Systems, devices and methods for analysis
US11434525B2 (en) 2020-08-06 2022-09-06 Singular Genomics Systems, Inc. Spatial sequencing
US11492662B2 (en) 2020-08-06 2022-11-08 Singular Genomics Systems, Inc. Methods for in situ transcriptomics and proteomics
EP4196581A4 (en) 2020-08-19 2024-05-29 Encodia, Inc. Sequential encoding methods and related kits
US11981958B1 (en) 2020-08-20 2024-05-14 10X Genomics, Inc. Methods for spatial analysis using DNA capture
CA3188680A1 (en) * 2020-08-21 2022-02-24 Arjun Ganesan Systems, devices and methods for determining most probable number in biological sample analysis
US11926822B1 (en) 2020-09-23 2024-03-12 10X Genomics, Inc. Three-dimensional spatial analysis
WO2022094148A1 (en) * 2020-10-30 2022-05-05 Encodia, Inc. Conjugation reagents and methods using 1,2-cyclohexanediones
EP4244382A1 (en) 2020-11-11 2023-09-20 Nautilus Subsidiary, Inc. Affinity reagents having enhanced binding and detection characteristics
US11827935B1 (en) 2020-11-19 2023-11-28 10X Genomics, Inc. Methods for spatial analysis using rolling circle amplification and detection probes
EP4121555A1 (en) 2020-12-21 2023-01-25 10X Genomics, Inc. Methods, compositions, and systems for capturing probes and/or barcodes
EP4278004A4 (en) * 2021-01-13 2024-10-30 The Regents of the University of Colorado, a body corporate Methods and compositions for molecular tracking in individual cells in vivo
CA3203106A1 (en) 2021-01-20 2022-07-28 Parag Mallick Systems and methods for biomolecule quantitation
CA3203535A1 (en) 2021-01-21 2022-07-28 Gregory KAPP Systems and methods for biomolecule preparation
IL305336B2 (en) 2021-03-11 2025-09-01 Nautilus Subsidiary Inc Systems and methods for preserving biomolecules
EP4428246B1 (en) 2021-04-14 2025-12-24 10X Genomics, Inc. Methods of measuring mislocalization of an analyte
EP4334706A4 (en) 2021-05-05 2025-08-06 Singular Genomics Systems Inc MULTIOMICS ANALYSIS DEVICE AND METHODS OF USE THEREOF
WO2022236054A1 (en) 2021-05-06 2022-11-10 10X Genomics, Inc. Methods for increasing resolution of spatial analysis
CN117396613A (zh) * 2021-06-01 2024-01-12 10X基因组学有限公司 用于分析物检测和探针解析的方法和组合物
EP4582555A3 (en) 2021-06-03 2025-10-22 10X Genomics, Inc. Methods, compositions, kits, and systems for enhancing analyte capture for spatial analysis
US20220412998A1 (en) 2021-06-24 2022-12-29 Nautilus Biotechnology, Inc. Methods and systems for assay refinement
US11521947B1 (en) * 2021-07-14 2022-12-06 Nxp Usa, Inc. Space efficient flip chip joint design
ES3011462T3 (en) 2021-09-01 2025-04-07 10X Genomics Inc Methods for blocking a capture probe on a spatial array
EP4399528A1 (en) 2021-09-09 2024-07-17 Nautilus Subsidiary, Inc. Characterization and localization of protein modifications
AU2022352593A1 (en) 2021-09-22 2024-02-15 Nautilus Subsidiary, Inc. Methods and systems for determining polypeptide interactions
US20230149883A1 (en) 2021-11-03 2023-05-18 Nautilus Biotechnology, Inc. Systems and methods for surface structuring
US12474346B2 (en) * 2021-11-05 2025-11-18 Encodia, Inc. Methods and kits for multicycle encoding assay
EP4419707A1 (en) 2021-11-10 2024-08-28 10X Genomics, Inc. Methods, compositions, and kits for determining the location of an analyte in a biological sample
US11753677B2 (en) * 2021-11-10 2023-09-12 Encodia, Inc. Methods for barcoding macromolecules in individual cells
EP4305195A2 (en) 2021-12-01 2024-01-17 10X Genomics, Inc. Methods, compositions, and systems for improved in situ detection of analytes and spatial analysis
KR20240137133A (ko) * 2021-12-14 2024-09-19 글리픽 바이오테크놀로지스, 인크. 분자 바코딩 및 엑스-시츄 분석을 통한 단일 분자 펩티드 서열분석
EP4206674B1 (en) 2021-12-28 2025-10-22 Encodia, Inc. High-throughput serotyping and antibody profiling assays
EP4500157A1 (en) 2022-03-29 2025-02-05 Nautilus Subsidiary, Inc. Integrated arrays for single-analyte processes
EP4515026A1 (en) 2022-04-25 2025-03-05 Nautilus Subsidiary, Inc. Systems and methods for assessing and improving the quality of multiplex molecular assays
EP4544077A1 (en) 2022-06-21 2025-04-30 Nautilus Subsidiary, Inc. Method for detecting analytes at sites of optically non-resolvable distances
JP2025527266A (ja) 2022-08-02 2025-08-20 グリフィック バイオテクノロジーズ，インク． 重合性分子の結合を介したタンパク質の配列決定
WO2024040236A2 (en) 2022-08-19 2024-02-22 Abrus Bio, Inc. Determination of protein information by recoding amino acid polymers into dna polymers
US20240087679A1 (en) 2022-09-13 2024-03-14 Nautilus Subsidiary, Inc. Systems and methods of validating new affinity reagents
US20240094215A1 (en) 2022-09-15 2024-03-21 Nautilus Subsidiary, Inc. Characterizing accessibility of macromolecule structures
WO2024073599A1 (en) 2022-09-29 2024-04-04 Nautilus Subsidiary, Inc. Preparation of array surfaces for single-analyte processes
CN115951051B (zh) * 2022-10-18 2024-01-12 北京卓诚惠生生物科技股份有限公司 一种高灵敏度新型冠状病毒抗原胶体金检测试剂盒及其制备方法
EP4630816A1 (en) 2022-12-09 2025-10-15 Nautilus Subsidiary, Inc. A method comprising performing on a single-analyte array at least 50 cycles of a process
EP4634285A1 (en) 2022-12-15 2025-10-22 Nautilus Subsidiary, Inc. Inhibition of photon phenomena on single molecule arrays
CN120752535A (zh) * 2023-01-05 2025-10-03 俄勒冈健康与科学大学 肽测序仪
CN120752349A (zh) * 2023-02-24 2025-10-03 小利兰·斯坦福大学理事会 对多肽进行测序的方法和相关组合物
US20240301469A1 (en) 2023-02-24 2024-09-12 Nautilus Subsidiary, Inc. Modifying, separating and detecting proteoforms
US20240344116A1 (en) 2023-03-24 2024-10-17 Nautilus Subsidiary, Inc. Transfer of nanoparticles to array surfaces
US20240394902A1 (en) 2023-05-24 2024-11-28 Nautilus Subsidiary, Inc. Systems and methods for machine vision
WO2024249961A1 (en) 2023-06-01 2024-12-05 Singular Genomics Systems, Inc. Methods and probes for detecting polynucleotide sequences in cells and tissues
US20240426839A1 (en) 2023-06-21 2024-12-26 Nautilus Subsidiary, Inc. Compositions and methods for improving affinity reagent avidity
WO2025043159A1 (en) 2023-08-24 2025-02-27 Nautilus Subsidiary, Inc. Compositions and methods for detecting binding interactions under equilibrium or non-equilibrium conditions
WO2025079184A1 (ja) * 2023-10-11 2025-04-17 中外製薬株式会社 複数のライブラリを用いたペプチドのスクリーニング方法
US20250154584A1 (en) * 2023-11-09 2025-05-15 Encodia, Inc. Methods for identifying polypeptides
US20250154579A1 (en) * 2023-11-12 2025-05-15 Interplay Bio System and method for high throughput screening of small molecule-protein interactions
US20250189519A1 (en) 2023-12-11 2025-06-12 Nautilus Subsidiary, Inc. Methods and compositions of particle-based arrays
WO2025137166A1 (en) 2023-12-20 2025-06-26 Nautilus Subsidiary, Inc. Fluidic devices for single-analyte assays
WO2025199236A1 (en) 2024-03-22 2025-09-25 Nautilus Subsidiary, Inc. Plasmonic particle systems for single-analyte assays
WO2025212338A1 (en) 2024-04-01 2025-10-09 Nautilus Subsidiary, Inc. Nanostructures for modulation of analyte conformation
WO2025247276A1 (zh) * 2024-05-28 2025-12-04 岚图生物科技(苏州)有限公司 一种偶联含有索引的pcr引物的微粒及其应用
CN118335197B (zh) * 2024-06-17 2024-09-24 南方科技大学 一种基于纳米孔测序芯片的dna数据存储方法
CN118988183B (zh) * 2024-08-09 2025-07-15 中国农业科学院油料作物研究所 一种富含甘油二酯的健康食用油制备方法及其产品
KR102804035B1 (ko) 2024-12-09 2025-05-09 주식회사 비씨젠 이차전지 캡 용접 장치
CN119851760B (zh) * 2025-01-14 2025-09-30 大连理工大学 一种基于三进制dna编码库的核酸检测方法

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000057183A1 (en) 1999-03-23 2000-09-28 Biovation Limited Protein isolation and analysis
US20080107660A1 (en) 2004-02-25 2008-05-08 Colin Self Binding Agents
WO2009050261A1 (en) 2007-10-19 2009-04-23 Pronota N.V. Preparation of samples for proteome analysis
US20090246879A1 (en) 2002-12-20 2009-10-01 Callida Genomics Materials and Methods Relating to Nano-Tags and Nano-Brands
US20090264300A1 (en) * 2005-12-01 2009-10-22 Nuevolution A/S Enzymatic encoding methods for efficient synthesis of large libraries
US20130052665A1 (en) 2011-08-25 2013-02-28 Bruce Xuefeng Ling Methods for diagnosis of systemic juvenile idiopathic arthritis
US20160253584A1 (en) 2015-02-27 2016-09-01 Cellular Research, Inc. Spatially addressable molecular barcoding

Family Cites Families (125)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4863870A (en) 1988-03-10 1989-09-05 Bio-Affinity Systems, Inc. Method of thioacylation peptide sequencing with alcoholysis of thiazolinones
US5484735A (en) 1989-08-23 1996-01-16 Northwestern University Immunoassay of glycosylated proteins employing antibody directed to reductively glycosylated N-terminal amino acids
US5049507A (en) 1989-12-21 1991-09-17 Applied Biosystems, Inc. Method of C-terminal peptide sequencing
EP0773227A1 (en) 1991-09-18 1997-05-14 Affymax Technologies N.V. Diverse collections of oligomers in use to prepare drugs, diagnostic reagents, pesticides or herbicides
US5573905A (en) 1992-03-30 1996-11-12 The Scripps Research Institute Encoded combinatorial chemical libraries
SE9400088D0 (sv) 1994-01-14 1994-01-14 Kabi Pharmacia Ab Bacterial receptor structures
US5834252A (en) 1995-04-18 1998-11-10 Glaxo Group Limited End-complementary polymerase reaction
CA2176414A1 (en) 1995-05-18 1996-11-19 S. David Kimball Acyl guanidine and amidine prodrugs
US20030198642A1 (en) 1995-08-03 2003-10-23 Johannes J. Geuze Cell derived antigen presenting vesicles
JP3124507B2 (ja) 1996-05-24 2001-01-15 セイコーインスツルメンツ株式会社 タンパク質あるいはペプチドのカルボキシ末端からのアミノ酸配列を決定する方法
US5900481A (en) 1996-11-06 1999-05-04 Sequenom, Inc. Bead linkers for immobilizing nucleic acids to solid supports
DE69835143T2 (de) 1997-01-21 2007-06-06 The General Hospital Corp., Boston Selektion von proteinen mittels rns-protein fusionen
US6306588B1 (en) 1997-02-07 2001-10-23 Invitrogen Corporation Polymerases for analyzing or typing polymorphic nucleic acid fragments and uses thereof
US6465193B2 (en) 1998-12-11 2002-10-15 The Regents Of The University Of California Targeted molecular bar codes and methods for using the same
US6875851B1 (en) 1999-03-05 2005-04-05 University Of Georgia Research Foundation, Inc. Prolyl tripeptidyl peptidases nucleic acid of Porphyromonas gingivalis
AU5723700A (en) 1999-05-25 2000-12-12 Praelux Incorporated Method for sequency and characterizing polymeric biomolecules using aptamers anda method for producing aptamers
CA2377468C (en) 1999-07-27 2010-04-20 Phylos, Inc. Peptide acceptor ligation methods
GB9927320D0 (en) 1999-11-18 2000-01-12 Chiron Spa Exosome separation
US7306904B2 (en) 2000-02-18 2007-12-11 Olink Ab Methods and kits for proximity probing
US6511809B2 (en) 2000-06-13 2003-01-28 E. I. Du Pont De Nemours And Company Method for the detection of an analyte by means of a nucleic acid reporter
CA2314398A1 (en) 2000-08-10 2002-02-10 Edward Shipwash Microarrays and microsystems for amino acid analysis and protein sequencing
EP1360490B1 (en) 2001-01-23 2011-12-21 President and Fellows of Harvard College Nucleic-acid programmable protein arrays
US20030087232A1 (en) * 2001-01-25 2003-05-08 Fred Christians Methods for screening polypeptides
US7727713B2 (en) 2001-06-20 2010-06-01 Nuevolution A/S Templated molecules and methods for using such molecules
AU2002330671B2 (en) 2001-07-06 2008-04-03 Centre National De La Recherche Scientifique (Cnrs) Method for obtaining cells with new properties
US7169894B2 (en) 2001-11-14 2007-01-30 Mirari Biosciences, Inc. Methods and compositions for reverse translation
AU2002353713A1 (en) 2001-11-23 2003-06-10 Simon Fredriksson Method and kit for proximity probing with multivalent proximity probes
DE10242016A1 (de) 2002-09-11 2004-03-25 Esplora GmbH c/o TU Darmstadt Institut für Biochemie Verfahren zur Identifizierung BHS-spezifischer Proteine und Fragmente davon
KR20050052666A (ko) 2002-10-10 2005-06-03 다이버사 코포레이션 프로테아제, 이를 암호화하는 핵산, 및 이를 제조하고사용하는 방법
WO2005015239A2 (en) 2003-08-07 2005-02-17 Cornell Research Foundation, Inc. Method for n-terminal labeling of proteins
US7198900B2 (en) 2003-08-29 2007-04-03 Applera Corporation Multiplex detection compositions, methods, and kits
ATE447626T1 (de) * 2003-09-18 2009-11-15 Nuevolution As Methode zur gewinnung struktureller informationen kodierter moleküle und zur selektion von verbindungen
EP1561755A2 (en) 2003-10-16 2005-08-10 Shimadzu Corporation Method for derivatizing protein or peptide to sulfonic acid derivative
EP1694693A2 (en) 2003-12-17 2006-08-30 Praecis Pharmaceuticals Inc. Methods for synthesis of encoded libraries
WO2005090566A2 (en) 2004-03-22 2005-09-29 Nuevolution A/S Ligational encoding using building block oligonucleotides
WO2005108615A2 (en) 2004-04-14 2005-11-17 President And Fellows Of Harvard College Nucleic-acid programmable protein arrays
US7393665B2 (en) 2005-02-10 2008-07-01 Population Genetics Technologies Ltd Methods and compositions for tagging and identifying polynucleotides
US20060199279A1 (en) 2005-03-07 2006-09-07 Viorica Lopez-Avila Methods for identifying post-translationally modified polypeptides
US7229769B2 (en) 2005-03-25 2007-06-12 Illumina, Inc. Compositions and methods for detecting protease activity
US7883848B2 (en) 2005-07-08 2011-02-08 Olink Ab Regulation analysis by cis reactivity, RACR
US20070020640A1 (en) 2005-07-21 2007-01-25 Mccloskey Megan L Molecular encoding of nucleic acid templates for PCR and other forms of sequence analysis
US7611834B2 (en) 2005-09-30 2009-11-03 Sandia Corporation Methods and devices for protein assays
US20070218503A1 (en) 2006-02-13 2007-09-20 Mitra Robi D Methods of polypeptide identification, and compositions therefor
DE102006015001A1 (de) 2006-03-31 2007-10-11 NMI Naturwissenschaftliches und Medizinisches Institut an der Universität Tübingen Verfahren zum Nachweis und/oder zur Anreicherung von Analytproteinen und/oder Analytpeptiden aus einer komplexen Proteinmischung
US8758991B2 (en) 2006-04-26 2014-06-24 University Of Louisville Research Foundation, Inc. Isolation of membrane vesicles from biological fluids and methods of using same
US8349167B2 (en) 2006-12-14 2013-01-08 Life Technologies Corporation Methods and apparatus for detecting molecular interactions using FET arrays
EP2191276B1 (en) 2007-08-03 2013-11-20 DKFZ Deutsches Krebsforschungszentrum Method for prenatal diagnosis using exosomes and cd24 as a marker
WO2010065322A1 (en) 2008-12-01 2010-06-10 Research Triangle Institute Concurrent identification of multitudes of polypeptides
WO2010065531A1 (en) 2008-12-01 2010-06-10 Robi David Mitra Single molecule protein screening
WO2010068278A2 (en) * 2008-12-10 2010-06-17 The Scripps Research Institute Production of carrier-peptide conjugates using chemically reactive unnatural amino acids
WO2010127186A1 (en) 2009-04-30 2010-11-04 Prognosys Biosciences, Inc. Nucleic acid constructs and methods of use
US9566335B1 (en) 2009-09-25 2017-02-14 The Governing Council Of The University Of Toronto Protein sequencing method and reagents
US9175340B2 (en) 2009-11-04 2015-11-03 President And Fellows Of Harvard College Reactivity-dependent and interaction-dependent PCR
US9371598B2 (en) * 2010-04-05 2016-06-21 Prognosys Biosciences, Inc. Spatially encoded biological assays
WO2011127006A1 (en) 2010-04-05 2011-10-13 Prognosys Biosciences, Inc. Co-localization affinity assays
CA2795776A1 (en) 2010-04-06 2011-10-13 Caris Life Sciences Luxembourg Holdings, S.A.R.L. Circulating biomarkers for disease
WO2011143583A1 (en) 2010-05-13 2011-11-17 Illumina, Inc. Binding assays for markers
US20130288929A1 (en) 2010-09-27 2013-10-31 Vipergen Method for Making an Enriched Library
EP2638057B1 (en) 2010-11-10 2019-03-06 Exosome Diagnostics, Inc. Method for isolation of nucleic acid containing particles and extraction of nucleic acids therefrom
GB201101621D0 (en) 2011-01-31 2011-03-16 Olink Ab Method and product
WO2012112804A1 (en) * 2011-02-18 2012-08-23 Raindance Technoligies, Inc. Compositions and methods for molecular labeling
EP3150750B1 (en) 2011-04-08 2018-12-26 Prognosys Biosciences, Inc. Peptide constructs and assay systems
CN103748236B (zh) 2011-04-15 2018-12-25 约翰·霍普金斯大学 安全测序系统
ES2744589T3 (es) 2011-05-11 2020-02-25 Exosome Diagnostics Inc Extracción de ácido nucleico de materiales biológicos heterogéneos
US9625469B2 (en) 2011-06-23 2017-04-18 Board Of Regents, The University Of Texas System Identifying peptides at the single molecule level
EP3492606B1 (en) 2011-08-22 2023-10-04 Exosome Diagnostics, Inc. Urine biomarkers
US9335292B2 (en) 2011-10-13 2016-05-10 Auburn University Electrochemical proximity assay
EP2776587A4 (en) 2011-11-10 2015-07-15 Exosome Diagnostics Inc LIQUOR ASSAY
US20150087526A1 (en) 2012-01-24 2015-03-26 The Regents Of The University Of Colorado, A Body Corporate Peptide identification and sequencing by single-molecule detection of peptides undergoing degradation
PL2814959T3 (pl) 2012-02-17 2018-07-31 Fred Hutchinson Cancer Research Center Kompozycje i sposoby do dokładnej identyfikacji mutacji
US9862995B2 (en) 2012-03-13 2018-01-09 Abhijit Ajit Patel Measurement of nucleic acid variants using highly-multiplexed error-suppressed deep sequencing
PT2828218T (pt) 2012-03-20 2020-11-11 Univ Washington Through Its Center For Commercialization Métodos para baixar a taxa de erro da sequenciação paralela massiva de adn utilizando sequenciação duplex de consensus
US9005888B2 (en) 2012-06-14 2015-04-14 System Biosciences, Llc Methods for microvesicle isolation and selective removal
NL2009191C2 (en) 2012-07-16 2014-01-20 Univ Delft Tech Single molecule protein sequencing.
EP2885418A4 (en) 2012-08-14 2016-03-02 10X Genomics Inc MICROCAPSE COMPOSITIONS AND METHOD THEREFOR
US20140378322A1 (en) 2012-08-14 2014-12-25 10X Technologies, Inc. Compositions and methods for sample processing
US9557336B2 (en) * 2012-09-07 2017-01-31 University Of Rochester Methods and compositions for site-specific labeling of peptides and proteins
CA2887058C (en) 2012-10-03 2022-02-22 Exosome Diagnostics, Inc. Use of microvesicles in diagnosis, prognosis, and treatment of medical diseases and conditions
ES2912033T3 (es) 2012-10-23 2022-05-24 Caris Science Inc Aptámeros y usos de los mismos
US10006919B2 (en) 2013-03-15 2018-06-26 Arizona Board Of Regents On Behalf Of Arizona State University Peptide array quality control
CA2905517C (en) 2013-03-15 2024-11-12 Lineage Biosciences, Inc. METHODS AND COMPOSITIONS FOR LABELING AND ANALYZING SAMPLES
WO2014142981A1 (en) 2013-03-15 2014-09-18 Illumina, Inc. Enzyme-linked nucleotides
US9435810B2 (en) 2013-03-15 2016-09-06 Washington University Molecules and methods for iterative polypeptide analysis and processing
US10053725B2 (en) 2013-04-23 2018-08-21 President And Fellows Of Harvard College In situ interaction determination
US10011830B2 (en) 2013-05-19 2018-07-03 The Board of Trustee of theLeland Stanford junior University Devices and methods for display of encoded peptides, polypeptides, and proteins on DNA
US20160122753A1 (en) * 2013-06-12 2016-05-05 Tarjei Mikkelsen High-throughput rna-seq
LT3013983T (lt) * 2013-06-25 2023-05-10 Prognosys Biosciences, Inc. Erdviniai koduoti biologiniai tyrimai, naudojant mikrofluidinį įrenginį
AU2014287071A1 (en) * 2013-07-10 2016-01-28 Children's Medical Center Corporation Compositions and methods relating to nucleic acid-protein complexes
CN105980615A (zh) 2013-08-28 2016-09-28 卡里斯生命科学瑞士控股有限公司 寡核苷酸探针及其用途
WO2015042506A1 (en) 2013-09-23 2015-03-26 The Trustees Of Columbia University In The City Of New York High-throughput single molecule protein identification
WO2015070037A2 (en) 2013-11-08 2015-05-14 Prognosys Biosciences, Inc. Polynucleotide conjugates and methods for analyte detection
US9834814B2 (en) 2013-11-22 2017-12-05 Agilent Technologies, Inc. Spatial molecular barcoding of in situ nucleic acids
CN106030304B (zh) 2014-02-27 2018-02-06 得克萨斯大学体系董事会 用于分离外来体的方法和组合物
WO2015148606A2 (en) 2014-03-25 2015-10-01 President And Fellows Of Harvard College Barcoded protein array for multiplex single-molecule interaction profiling
JP6853667B2 (ja) 2014-04-21 2021-03-31 プレジデント アンド フェローズ オブ ハーバード カレッジ 核酸をバーコーディングするためのシステムおよび方法
EP3134427B1 (en) 2014-04-24 2020-06-03 Nanyang Technological University Asx-specific protein ligase
JP6593710B2 (ja) 2014-05-02 2019-10-23 Ｉｄａｃセラノスティクス株式会社 単一細胞由来核酸の解析方法
EP3143165A4 (en) 2014-05-15 2018-05-16 Two Pore Guys, Inc. Scaffold data storage and target detection in a sample using a nanopore
MX2016016902A (es) 2014-06-26 2017-03-27 10X Genomics Inc Metodos para analizar acidos nucleicos de celulas individuales o poblaciones de celulas.
WO2015200869A1 (en) * 2014-06-26 2015-12-30 10X Genomics, Inc. Analysis of nucleic acid sequences
WO2016004068A1 (en) 2014-06-30 2016-01-07 Jae-Won Shin Hydrogel compositions comprising encapsulated cells and methods of use thereof
US10465183B2 (en) 2014-07-09 2019-11-05 Exosome Diagnostics, Inc. Methods for isolating microvesicles and extracting nucleic acids from biological samples
EP3169812A4 (en) 2014-07-18 2017-12-20 CDI Laboratories Inc. Methods and compositions to identify, quantify, and characterize target analytes and binding moieties
CA2956925C (en) 2014-08-01 2024-02-13 Dovetail Genomics, Llc Tagging nucleic acids for sequence assembly
US10545153B2 (en) 2014-09-15 2020-01-28 Board Of Regents, The University Of Texas System Single molecule peptide sequencing
JP6808617B2 (ja) 2014-10-17 2021-01-06 イルミナ ケンブリッジ リミテッド 連続性を維持した転位
US9771412B2 (en) 2014-10-31 2017-09-26 Wisconsin Alumni Research Foundation Engineered intein for improved production of protein-intein fusions
EP3725893A1 (en) 2015-02-10 2020-10-21 Illumina, Inc. Compositions for analyzing cellular components
WO2016145416A2 (en) 2015-03-11 2016-09-15 The Broad Institute, Inc. Proteomic analysis with nucleic acid identifiers
WO2016164530A1 (en) 2015-04-07 2016-10-13 University Of Maryland, College Park Compositions and methods for high throughput protein sequencing
CA2988771A1 (en) 2015-06-09 2016-12-15 The Board Of Regents Of The University Of Texas System Diagnostic test for early stage cancer
US11965891B2 (en) 2015-12-30 2024-04-23 Bio-Rad Laboratories, Inc. Digital protein quantification
EP3916107B1 (en) 2016-02-17 2023-04-05 President and Fellows of Harvard College Molecular programming tools
MX2018013341A (es) 2016-05-02 2019-09-18 Encodia Inc Analisis de macromoleculas que emplea la codificacion de acido nucleico.
EP3464636B1 (en) 2016-05-27 2022-07-06 President and Fellows of Harvard College Conditional primer extension for single-molecule detection
WO2018005720A1 (en) 2016-06-30 2018-01-04 President And Fellows Of Harvard College Method of determining the molecular binding between libraries of molecules
CN109790574A (zh) 2016-07-22 2019-05-21 哈佛学院院长及董事 用于蛋白质鉴别的方法和组合物
CN110214186B (zh) 2017-01-30 2023-11-24 10X基因组学有限公司 用于基于微滴的单细胞条形编码的方法和系统
EP3583214B1 (en) 2017-02-02 2023-11-22 New York Genome Center, Inc. Methods and compositions for identifying or quantifying targets in a biological sample
CA3081446A1 (en) 2017-10-31 2019-05-09 Encodia, Inc. Methods and compositions for polypeptide analysis
SG11202003923PA (en) 2017-10-31 2020-05-28 Encodia Inc Methods and kits using nucleic acid encoding and/or label
EP3704249B1 (en) 2017-10-31 2025-05-21 Encodia, Inc. Kits for analysis using nucleic acid encoding and/or label
MX2020006803A (es) 2017-12-29 2020-10-28 Nautilus Biotechnology Inc Estrategias de descodificacion para identificacion de proteinas.
EP3740567A4 (en) 2018-01-18 2022-03-09 Nanosomix Inc. DETECTION OF EXOSOMES AND EXOSOMAL BIOMARKERS FOR DIAGNOSIS AND PROGNOSIS OF DISEASES AND DISORDERS
US12270741B2 (en) 2018-04-03 2025-04-08 Tymora Analytical Operations, Llc Methods for affinity-based non-antibody capture and purification of extracellular vesicles

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000057183A1 (en) 1999-03-23 2000-09-28 Biovation Limited Protein isolation and analysis
US20090246879A1 (en) 2002-12-20 2009-10-01 Callida Genomics Materials and Methods Relating to Nano-Tags and Nano-Brands
US20080107660A1 (en) 2004-02-25 2008-05-08 Colin Self Binding Agents
US20090264300A1 (en) * 2005-12-01 2009-10-22 Nuevolution A/S Enzymatic encoding methods for efficient synthesis of large libraries
WO2009050261A1 (en) 2007-10-19 2009-04-23 Pronota N.V. Preparation of samples for proteome analysis
US20130052665A1 (en) 2011-08-25 2013-02-28 Bruce Xuefeng Ling Methods for diagnosis of systemic juvenile idiopathic arthritis
US20160253584A1 (en) 2015-02-27 2016-09-01 Cellular Research, Inc. Spatially addressable molecular barcoding

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