JP7120630B2 - 核酸エンコーディングを使用した巨大分子解析 - Google Patents
核酸エンコーディングを使用した巨大分子解析 Download PDFInfo
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- JP7120630B2 JP7120630B2 JP2018558133A JP2018558133A JP7120630B2 JP 7120630 B2 JP7120630 B2 JP 7120630B2 JP 2018558133 A JP2018558133 A JP 2018558133A JP 2018558133 A JP2018558133 A JP 2018558133A JP 7120630 B2 JP7120630 B2 JP 7120630B2
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
- G01N33/6818—Sequencing of polypeptides
- G01N33/6824—Sequencing of polypeptides involving N-terminal degradation, e.g. Edman degradation
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
- G01N33/6818—Sequencing of polypeptides
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6402—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from non-mammals
- C12N9/6405—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from non-mammals not being snakes
- C12N9/6416—Metalloendopeptidases (3.4.24)
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6804—Nucleic acid analysis using immunogens
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
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- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B20/00—Methods specially adapted for identifying library members
- C40B20/04—Identifying library members by means of a tag, label, or other readable or detectable entity associated with the library members, e.g. decoding processes
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- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B30/00—Methods of screening libraries
- C40B30/04—Methods of screening libraries by measuring the ability to specifically bind a target molecule, e.g. antibody-antigen binding, receptor-ligand binding
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- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B40/00—Libraries per se, e.g. arrays, mixtures
- C40B40/04—Libraries containing only organic compounds
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- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B40/00—Libraries per se, e.g. arrays, mixtures
- C40B40/04—Libraries containing only organic compounds
- C40B40/10—Libraries containing peptides or polypeptides, or derivatives thereof
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- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B70/00—Tags or labels specially adapted for combinatorial chemistry or libraries, e.g. fluorescent tags or bar codes
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
- G01N33/6842—Proteomic analysis of subsets of protein mixtures with reduced complexity, e.g. membrane proteins, phosphoproteins, organelle proteins
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
- G01N33/6845—Methods of identifying protein-protein interactions in protein mixtures
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
- C12Q2565/60—Detection means characterised by use of a special device
- C12Q2565/619—Detection means characterised by use of a special device being a video camera
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2458/00—Labels used in chemical analysis of biological material
- G01N2458/10—Oligonucleotides as tagging agents for labelling antibodies
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2570/00—Omics, e.g. proteomics, glycomics or lipidomics; Methods of analysis focusing on the entire complement of classes of biological molecules or subsets thereof, i.e. focusing on proteomes, glycomes or lipidomes
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Organic Chemistry (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Medicinal Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biophysics (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Analytical Chemistry (AREA)
- Bioinformatics & Computational Biology (AREA)
- Pathology (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- General Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- General Engineering & Computer Science (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Peptides Or Proteins (AREA)
- Information Retrieval, Db Structures And Fs Structures Therefor (AREA)
Applications Claiming Priority (7)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201662330841P | 2016-05-02 | 2016-05-02 | |
| US62/330,841 | 2016-05-02 | ||
| US201662339071P | 2016-05-19 | 2016-05-19 | |
| US62/339,071 | 2016-05-19 | ||
| US201662376886P | 2016-08-18 | 2016-08-18 | |
| US62/376,886 | 2016-08-18 | ||
| PCT/US2017/030702 WO2017192633A1 (en) | 2016-05-02 | 2017-05-02 | Macromolecule analysis employing nucleic acid encoding |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2020081385A Division JP7097627B2 (ja) | 2016-05-02 | 2020-05-01 | 核酸エンコーディングを使用した巨大分子解析 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2019523635A JP2019523635A (ja) | 2019-08-29 |
| JP2019523635A5 JP2019523635A5 (enExample) | 2020-06-18 |
| JP7120630B2 true JP7120630B2 (ja) | 2022-08-17 |
Family
ID=60203299
Family Applications (5)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2018558133A Active JP7120630B2 (ja) | 2016-05-02 | 2017-05-02 | 核酸エンコーディングを使用した巨大分子解析 |
| JP2020081385A Active JP7097627B2 (ja) | 2016-05-02 | 2020-05-01 | 核酸エンコーディングを使用した巨大分子解析 |
| JP2022025551A Active JP7333975B2 (ja) | 2016-05-02 | 2022-02-22 | 核酸エンコーディングを使用した巨大分子解析 |
| JP2022106383A Withdrawn JP2022126865A (ja) | 2016-05-02 | 2022-06-30 | 核酸エンコーディングを使用した巨大分子解析 |
| JP2024006005A Pending JP2024041969A (ja) | 2016-05-02 | 2024-01-18 | 核酸エンコーディングを使用した巨大分子解析 |
Family Applications After (4)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2020081385A Active JP7097627B2 (ja) | 2016-05-02 | 2020-05-01 | 核酸エンコーディングを使用した巨大分子解析 |
| JP2022025551A Active JP7333975B2 (ja) | 2016-05-02 | 2022-02-22 | 核酸エンコーディングを使用した巨大分子解析 |
| JP2022106383A Withdrawn JP2022126865A (ja) | 2016-05-02 | 2022-06-30 | 核酸エンコーディングを使用した巨大分子解析 |
| JP2024006005A Pending JP2024041969A (ja) | 2016-05-02 | 2024-01-18 | 核酸エンコーディングを使用した巨大分子解析 |
Country Status (12)
| Country | Link |
|---|---|
| US (10) | US12123878B2 (enExample) |
| EP (2) | EP3452591B1 (enExample) |
| JP (5) | JP7120630B2 (enExample) |
| KR (3) | KR102741603B1 (enExample) |
| CN (3) | CN110199019B (enExample) |
| AU (1) | AU2017259794B2 (enExample) |
| CA (1) | CA3022863A1 (enExample) |
| DK (1) | DK3452591T5 (enExample) |
| MX (1) | MX2018013341A (enExample) |
| NZ (1) | NZ747941A (enExample) |
| SG (2) | SG10201911917WA (enExample) |
| WO (1) | WO2017192633A1 (enExample) |
Families Citing this family (171)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20190300945A1 (en) | 2010-04-05 | 2019-10-03 | Prognosys Biosciences, Inc. | Spatially Encoded Biological Assays |
| US11435358B2 (en) | 2011-06-23 | 2022-09-06 | Board Of Regents, The University Of Texas System | Single molecule peptide sequencing |
| DE112012002570B4 (de) | 2011-06-23 | 2023-11-23 | Board Of Regents, The University Of Texas System | Identifizieren von Peptiden auf der Einzelmolekülebene |
| DK3511423T4 (da) | 2012-10-17 | 2024-07-29 | Spatial Transcriptomics Ab | Fremgangsmåder og produkt til optimering af lokaliseret eller rumlig detektion af genekspression i en vævsprøve |
| EP3543703A1 (en) | 2014-09-15 | 2019-09-25 | Board of Regents, The University of Texas System | Improved single molecule peptide sequencing |
| SG11201705615UA (en) | 2015-01-12 | 2017-08-30 | 10X Genomics Inc | Processes and systems for preparing nucleic acid sequencing libraries and libraries prepared using same |
| EP4119677B1 (en) | 2015-04-10 | 2023-06-28 | Spatial Transcriptomics AB | Spatially distinguished, multiplex nucleic acid analysis of biological specimens |
| WO2016210348A2 (en) | 2015-06-26 | 2016-12-29 | Ancera, Inc. | Background defocusing and clearing in ferrofluid-based capture assays |
| KR102741603B1 (ko) | 2016-05-02 | 2024-12-16 | 엔코디아, 인코포레이티드 | 암호화 핵산을 사용한 거대분자 분석 |
| US10202641B2 (en) | 2016-05-31 | 2019-02-12 | Cellular Research, Inc. | Error correction in amplification of samples |
| WO2018017914A1 (en) * | 2016-07-22 | 2018-01-25 | President And Fellows Of Harvard College | Methods and compositions for protein identification |
| KR102638006B1 (ko) * | 2016-09-26 | 2024-02-20 | 셀룰러 리서치, 인크. | 바코딩된 올리고뉴클레오티드 서열을 갖는 시약을 이용한 단백질 발현의 측정 |
| KR20230054484A (ko) | 2016-11-16 | 2023-04-24 | 카탈로그 테크놀로지스, 인크. | 핵산-기반 데이터 저장용 시스템 |
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| EP3548652B1 (en) | 2016-12-01 | 2024-04-03 | Nautilus Subsidiary, Inc. | Methods of assaying proteins |
| DK3929304T3 (da) * | 2016-12-16 | 2024-04-08 | Aratome Llc | Molekylær detektering ved ligeringsforstærkning |
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| GB201707140D0 (en) * | 2017-05-04 | 2017-06-21 | Oxford Nanopore Tech Ltd | Method |
| EP4345172A3 (en) | 2017-06-05 | 2024-07-03 | Becton, Dickinson and Company | Sample indexing for single cells |
| GB201715684D0 (en) * | 2017-09-28 | 2017-11-15 | Univ Gent | Means and methods for single molecule peptide sequencing |
| US20200256862A1 (en) * | 2017-10-23 | 2020-08-13 | Massachusetts Institute Of Technology | Functionalized solid support |
| SG11202003923PA (en) | 2017-10-31 | 2020-05-28 | Encodia Inc | Methods and kits using nucleic acid encoding and/or label |
| KR102556494B1 (ko) | 2017-10-31 | 2023-07-18 | 엔코디아, 인코포레이티드 | 핵산 암호화 및/또는 표지를 이용한 분석용 키트 |
| WO2019104155A2 (en) * | 2017-11-22 | 2019-05-31 | The University Of Chicago | Chemical probe-dependent evaluation of protein activity and uses thereof |
| CA3086915A1 (en) | 2017-12-29 | 2019-07-04 | Sujal M. Patel | Decoding approaches for protein identification |
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| AU2019262048B2 (en) | 2018-05-03 | 2025-09-04 | Becton, Dickinson And Company | High throughput multiomics sample analysis |
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| US12385083B2 (en) | 2018-12-10 | 2025-08-12 | 10X Genomics, Inc. | Methods of using master / copy arrays for spatial detection |
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