KR100270644B1 - 서브틸리신 타입의 아스퍼질러스 세린 프로테아제,이를 암호화 하는 dna 서열 및 이들의 제조방법 - Google Patents

서브틸리신 타입의 아스퍼질러스 세린 프로테아제,이를 암호화 하는 dna 서열 및 이들의 제조방법 Download PDF

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KR100270644B1
KR100270644B1 KR1019930006178A KR930006178A KR100270644B1 KR 100270644 B1 KR100270644 B1 KR 100270644B1 KR 1019930006178 A KR1019930006178 A KR 1019930006178A KR 930006178 A KR930006178 A KR 930006178A KR 100270644 B1 KR100270644 B1 KR 100270644B1
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South Korea
Prior art keywords
aspergillus niger
aspergillus
dna
gene
subtilisin
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KR1019930006178A
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English (en)
Korean (ko)
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KR930021783A (ko
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프랭크벅스톤
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더블류. 하링, 지. 보이롤
노바티스 아게
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Priority claimed from GB939305097A external-priority patent/GB9305097D0/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
    • C12N9/62—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi from Aspergillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Mycology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Detergent Compositions (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
KR1019930006178A 1992-04-15 1993-04-14 서브틸리신 타입의 아스퍼질러스 세린 프로테아제,이를 암호화 하는 dna 서열 및 이들의 제조방법 Expired - Lifetime KR100270644B1 (ko)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
EP92810281 1992-04-15
EP92810281.3 1992-04-15
GB939305097A GB9305097D0 (en) 1993-03-12 1993-03-12 Noval fungal protease
GB9305097.9 1993-03-12

Publications (2)

Publication Number Publication Date
KR930021783A KR930021783A (ko) 1993-11-23
KR100270644B1 true KR100270644B1 (ko) 2000-11-01

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KR1019930006178A Expired - Lifetime KR100270644B1 (ko) 1992-04-15 1993-04-14 서브틸리신 타입의 아스퍼질러스 세린 프로테아제,이를 암호화 하는 dna 서열 및 이들의 제조방법

Country Status (17)

Country Link
EP (1) EP0574347B1 (enrdf_load_stackoverflow)
JP (2) JP3424840B2 (enrdf_load_stackoverflow)
KR (1) KR100270644B1 (enrdf_load_stackoverflow)
AT (1) ATE252156T1 (enrdf_load_stackoverflow)
AU (1) AU663173B2 (enrdf_load_stackoverflow)
CA (1) CA2093950C (enrdf_load_stackoverflow)
CY (1) CY2507B1 (enrdf_load_stackoverflow)
DE (1) DE69333249T2 (enrdf_load_stackoverflow)
DK (1) DK0574347T3 (enrdf_load_stackoverflow)
ES (1) ES2208637T3 (enrdf_load_stackoverflow)
FI (1) FI112667B (enrdf_load_stackoverflow)
HU (1) HU219809B (enrdf_load_stackoverflow)
IL (2) IL105383A0 (enrdf_load_stackoverflow)
MX (1) MX9302202A (enrdf_load_stackoverflow)
NO (1) NO315379B1 (enrdf_load_stackoverflow)
NZ (1) NZ247397A (enrdf_load_stackoverflow)
PT (1) PT574347E (enrdf_load_stackoverflow)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5821350A (en) * 1995-11-01 1998-10-13 Nexia Biotechnologies, Inc. Aspergillus niger beta-galactosidase gene
AU1092897A (en) * 1995-12-15 1997-07-14 Novo Nordisk A/S A funguns wherein the area, pepc and/or pepe genes have been inactivated
WO1997035956A1 (en) * 1996-03-27 1997-10-02 Novo Nordisk A/S Alkaline protease deficient filamentous fungi
WO1997046689A1 (en) * 1996-06-05 1997-12-11 Gist-Brocades B.V. Fungal metallo protease genes
JPH1016992A (ja) * 1996-07-04 1998-01-20 Toagosei Co Ltd 接着剤用容器
US6806062B1 (en) 1998-10-05 2004-10-19 Novozymes A/S Fungal transcriptional activator useful in methods for producing polypeptides
CN1425068B (zh) 2000-03-14 2011-05-18 诺维信公司 在制备多肽的方法中有效的真菌转录激活因子
WO2007045248A1 (en) 2005-10-17 2007-04-26 Novozymes A/S Use of fungal mutants for expression of antibodies
US8633010B2 (en) 2007-12-06 2014-01-21 Novozymes A/S Fungal PepC inhibitor
CN102762714B (zh) 2009-12-18 2015-11-25 诺维信股份有限公司 用于在木霉属的蛋白酶缺陷突变体中产生多肽的方法
MD4186C1 (ro) * 2012-02-20 2013-06-30 Институт Микробиологии И Биотехнологии Академии Наук Молдовы Tulpină de fungi Fusarium gibbosum - producătoare de proteaze acide şi neutre, xilanaze şi b-glucozidaze
US10435731B2 (en) 2013-07-10 2019-10-08 Glykos Finland Oy Multiple proteases deficient filamentous fungal cells and methods of use thereof
US10619144B2 (en) 2013-08-23 2020-04-14 Novozymes A/S Regulated PepC expression
SG11201700446XA (en) 2014-07-21 2017-02-27 Glykos Finland Oy Production of glycoproteins with mammalian-like n-glycans in filamentous fungi
CN116121151A (zh) * 2023-03-20 2023-05-16 广东溢多利生物科技股份有限公司 黑曲霉突变株anpep15m1及其应用

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990000192A1 (en) * 1988-07-01 1990-01-11 Genencor, Inc. Aspartic proteinase deficient filamentous fungi

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990000192A1 (en) * 1988-07-01 1990-01-11 Genencor, Inc. Aspartic proteinase deficient filamentous fungi

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Chem Pharm Bull, vol. 37, pp. 1333-36 (1989) *

Also Published As

Publication number Publication date
NO315379B1 (no) 2003-08-25
EP0574347A3 (enrdf_load_stackoverflow) 1994-04-13
DE69333249T2 (de) 2004-07-29
JP2003235591A (ja) 2003-08-26
PT574347E (pt) 2004-02-27
HU219809B (hu) 2001-08-28
AU663173B2 (en) 1995-09-28
AU3695993A (en) 1993-10-21
EP0574347B1 (en) 2003-10-15
FI112667B (fi) 2003-12-31
JPH0646863A (ja) 1994-02-22
MX9302202A (es) 1994-04-29
CY2507B1 (en) 2005-12-23
CA2093950A1 (en) 1993-10-16
FI931634A0 (fi) 1993-04-08
JP3636455B2 (ja) 2005-04-06
IL105383A0 (en) 1993-08-18
KR930021783A (ko) 1993-11-23
ATE252156T1 (de) 2003-11-15
CA2093950C (en) 2006-05-09
IL170763A (en) 2007-10-31
NO931368D0 (no) 1993-04-14
EP0574347A2 (en) 1993-12-15
JP3424840B2 (ja) 2003-07-07
NZ247397A (en) 1995-08-28
DE69333249D1 (de) 2003-11-20
ES2208637T3 (es) 2004-06-16
DK0574347T3 (da) 2004-01-26
NO931368L (no) 1993-10-18
HU9301087D0 (en) 1993-06-28
FI931634L (fi) 1993-10-16
HUT67800A (en) 1995-05-29

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