JPH10512959A - 多光子レーザ顕微鏡法 - Google Patents
多光子レーザ顕微鏡法Info
- Publication number
- JPH10512959A JPH10512959A JP9512766A JP51276697A JPH10512959A JP H10512959 A JPH10512959 A JP H10512959A JP 9512766 A JP9512766 A JP 9512766A JP 51276697 A JP51276697 A JP 51276697A JP H10512959 A JPH10512959 A JP H10512959A
- Authority
- JP
- Japan
- Prior art keywords
- photons
- excitation
- laser
- photon
- light
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Ceased
Links
Classifications
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/0004—Microscopes specially adapted for specific applications
- G02B21/002—Scanning microscopes
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/0004—Microscopes specially adapted for specific applications
- G02B21/002—Scanning microscopes
- G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
- G02B21/0052—Optical details of the image generation
- G02B21/0076—Optical details of the image generation arrangements using fluorescence or luminescence
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/0004—Microscopes specially adapted for specific applications
- G02B21/002—Scanning microscopes
- G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
- G02B21/008—Details of detection or image processing, including general computer control
- G02B21/0084—Details of detection or image processing, including general computer control time-scale detection, e.g. strobed, ultra-fast, heterodyne detection
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/6456—Spatial resolved fluorescence measurements; Imaging
- G01N21/6458—Fluorescence microscopy
Landscapes
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Optics & Photonics (AREA)
- Computer Vision & Pattern Recognition (AREA)
- Engineering & Computer Science (AREA)
- General Engineering & Computer Science (AREA)
- Microscoopes, Condenser (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Lasers (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Optical Integrated Circuits (AREA)
Abstract
Description
Claims (1)
- 【特許請求の範囲】 1. 特性エネルギーの光子により励起可能な分子を含む目的物の3つ又はそ れ以上の光子の励起技術による顕微鏡検査法であって、 nが上記目的物により吸収されるべき光子の数に等しく、3より大きい若しく はそれに等しい場合に、上記目的物に、上記特性エネルギーの約1/nのエネル ギーの光子を有するレーザ光の強いサブピコ秒のパルスのビームを照射し、 少なくとも3つの入射光子の同時吸収により分子励起を生じさせるべく、十分 に高い照射強度をもたらすように、上記目的物の範囲内に照射光を結像させるス テップを有する方法。 2. 上記目的物が、かご閉じ込めの生物学的に活性な分子を含み、上記照射 強度が、n個の入射光子の同時吸収により、かご閉じ込めされた生物学的に活性 な化合物を解放するのに十分であり、各エネルギーが、上記特性エネルギーの約 1/nに等しい請求の範囲第1項の方法。 3. 上記目的物が、蛍光性の分子を含み、上記照射強度が、n個の入射光子 7の同時吸収により、上記目的物の蛍光を生じさせるのに十分であり、各エネル ギーが、上記特性エネルギーの約1/nに等しい請求の範囲第1項の方法。 4. 上記照射光を結像させるステップが、上記n個の入射光子の同時吸収に より分子励起を生じさせるべく、上記焦点体積においてのみ十分に高い照射強度 をもたらすように、上記目的物の範囲内における小さな焦点体積へ照射光を結像 させることを有している請求の範囲第3項の方法。 5. 上記目的物を通じて、上記焦点体積を走査すべく、上記ビームを走査し 、 上記目的物により生じさせられた蛍光を検出するステップを更に含む請求の範 囲第4項の方法。 6. 上記目的物が組織であり、また、上記分子励起が、内在的な発色団,外 部から与えられた発色団、若しくは上記組織内の内在的な蛍光体による少なくと も3つの照射光子の同時吸収により発生する請求の範囲第1項の方法。 7. 蛍光光子を生じさせるための特性エネルギーの光子による励起に応答す る蛍光成分を含む目的物のレーザ走査蛍光顕微鏡検査用の装置であって、 上記目的物を受合うためのステージ手段と、 nが3よりも大きい、あるいはそれに等しい場合に、上記特性エネルギーの1 /nのエネルギーで構成されるサブピコ秒のコヒーレント光パルスの少なくとも 1つの光源と、 上記目的物に少なくとも3つの光子を吸収させ、上記蛍光光子を生じさせるべ く、上記目的物上にコヒーレント光パルスを結像させる手段と、 上記蛍光光子を検出する検出手段と、 上記蛍光光子を検出手段に対して指向させる手段とを備えた装置。 8. 上記目的物を通じて、上記光パルスを走査する走査手段を更に備えた請 求の範囲第7項の装置。 9. 上記検出手段が、共焦点光電子増倍管を有する請求の範囲第7項の装置 。 10. 上記検出手段が、フーリエ平面検出器を有する請求の範囲第7項の装 置。 11. 上記検出手段が、画像形成検出器を有する請求の範囲第7項の装置。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US395795P | 1995-09-19 | 1995-09-19 | |
US60/003,957 | 1995-09-19 | ||
PCT/US1996/014519 WO1997011355A1 (en) | 1995-09-19 | 1996-09-18 | Multi-photon laser microscopy |
Related Child Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2001229511A Division JP2002139436A (ja) | 1995-09-19 | 2001-07-30 | 多光子レーザ顕微鏡法 |
Publications (1)
Publication Number | Publication Date |
---|---|
JPH10512959A true JPH10512959A (ja) | 1998-12-08 |
Family
ID=21708403
Family Applications (3)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP9512766A Ceased JPH10512959A (ja) | 1995-09-19 | 1996-09-18 | 多光子レーザ顕微鏡法 |
JP2001229511A Pending JP2002139436A (ja) | 1995-09-19 | 2001-07-30 | 多光子レーザ顕微鏡法 |
JP2005322027A Pending JP2006106004A (ja) | 1995-09-19 | 2005-11-07 | 多光子レーザ顕微鏡法 |
Family Applications After (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2001229511A Pending JP2002139436A (ja) | 1995-09-19 | 2001-07-30 | 多光子レーザ顕微鏡法 |
JP2005322027A Pending JP2006106004A (ja) | 1995-09-19 | 2005-11-07 | 多光子レーザ顕微鏡法 |
Country Status (9)
Country | Link |
---|---|
US (2) | US6166385A (ja) |
EP (1) | EP0852716B1 (ja) |
JP (3) | JPH10512959A (ja) |
AU (1) | AU700560B2 (ja) |
CA (1) | CA2231222C (ja) |
DE (2) | DE852716T1 (ja) |
NO (1) | NO981032L (ja) |
NZ (1) | NZ318277A (ja) |
WO (1) | WO1997011355A1 (ja) |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
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JP2000347229A (ja) * | 1999-04-27 | 2000-12-15 | Carl Zeiss Jena Gmbh | 顕微鏡における短パルスレーザーのレーザー出力および/またはパルス幅調整のための配置 |
JP2003057554A (ja) * | 2001-08-09 | 2003-02-26 | Olympus Optical Co Ltd | レーザ顕微鏡 |
JP2007538289A (ja) * | 2004-05-19 | 2007-12-27 | ゲーリー、ブルッカー | 共焦点励起平面を有した広視野多光子顕微鏡のための方法およびシステム |
US7342219B2 (en) | 2004-06-01 | 2008-03-11 | Olympus Corporation | Laser scanning microscope |
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DE19851240C1 (de) * | 1998-11-06 | 2000-03-02 | Europ Lab Molekularbiolog | Fluoreszenzmikroskop mit Mehrphotonenanregung |
DE19908883A1 (de) | 1999-03-02 | 2000-09-07 | Rainer Heintzmann | Verfahren zur Erhöhung der Auflösung optischer Abbildung |
ATE235686T1 (de) * | 1999-04-29 | 2003-04-15 | Univ Erasmus | Bestimmung von analytbeweglichkeit |
US6555781B2 (en) * | 1999-05-10 | 2003-04-29 | Nanyang Technological University | Ultrashort pulsed laser micromachining/submicromachining using an acoustooptic scanning device with dispersion compensation |
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DE19935766A1 (de) * | 1999-07-29 | 2001-02-01 | Friedrich Schiller Uni Jena Bu | Verfahren zur optischen Anregung von Fluorophor-markierter DNA und RNA |
DE19939706C2 (de) * | 1999-08-18 | 2002-09-05 | Forschungsverbund Berlin Ev | Fluorophor für die Multi-Photonen-Laser-Scanning-Mikroskopie |
DE19950692A1 (de) * | 1999-10-15 | 2001-04-19 | Zeiss Carl Jena Gmbh | Anordnung zur wellenlängenabhängigen Einstellung von Lichtstrahlung |
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JP2000347229A (ja) * | 1999-04-27 | 2000-12-15 | Carl Zeiss Jena Gmbh | 顕微鏡における短パルスレーザーのレーザー出力および/またはパルス幅調整のための配置 |
JP4701474B2 (ja) * | 1999-04-27 | 2011-06-15 | カール ツアイス マイクロイメージング ゲゼルシャフト ミット ベシュレンクテル ハフツング | 顕微鏡における短パルスレーザーのレーザー出力および/またはパルス長調整のための装置 |
JP2003057554A (ja) * | 2001-08-09 | 2003-02-26 | Olympus Optical Co Ltd | レーザ顕微鏡 |
JP2007538289A (ja) * | 2004-05-19 | 2007-12-27 | ゲーリー、ブルッカー | 共焦点励起平面を有した広視野多光子顕微鏡のための方法およびシステム |
US7342219B2 (en) | 2004-06-01 | 2008-03-11 | Olympus Corporation | Laser scanning microscope |
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AU6972496A (en) | 1997-04-09 |
EP0852716B1 (en) | 2005-11-30 |
CA2231222A1 (en) | 1997-03-27 |
NO981032D0 (no) | 1998-03-09 |
JP2006106004A (ja) | 2006-04-20 |
DE69635521T2 (de) | 2006-08-17 |
EP0852716A4 (en) | 1999-01-13 |
DE852716T1 (de) | 2001-07-19 |
AU700560B2 (en) | 1999-01-07 |
EP0852716A1 (en) | 1998-07-15 |
US6166385A (en) | 2000-12-26 |
CA2231222C (en) | 2001-12-11 |
WO1997011355A1 (en) | 1997-03-27 |
JP2002139436A (ja) | 2002-05-17 |
NZ318277A (en) | 1999-02-25 |
NO981032L (no) | 1998-05-04 |
US6344653B1 (en) | 2002-02-05 |
DE69635521D1 (de) | 2006-01-05 |
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