JPH0134599B2 - - Google Patents

Info

Publication number
JPH0134599B2
JPH0134599B2 JP56213193A JP21319381A JPH0134599B2 JP H0134599 B2 JPH0134599 B2 JP H0134599B2 JP 56213193 A JP56213193 A JP 56213193A JP 21319381 A JP21319381 A JP 21319381A JP H0134599 B2 JPH0134599 B2 JP H0134599B2
Authority
JP
Japan
Prior art keywords
plasmid
trp
promoter
escherichia coli
atcc
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
JP56213193A
Other languages
English (en)
Japanese (ja)
Other versions
JPS58110600A (ja
Inventor
Seiga Ito
Tatsuya Nishi
Tamio Mizukami
Tadashi Matsumoto
Tetsuo Oka
Koreaki Taniguchi
Haruo Sugano
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
KH Neochem Co Ltd
Original Assignee
Kyowa Hakko Kogyo Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kyowa Hakko Kogyo Co Ltd filed Critical Kyowa Hakko Kogyo Co Ltd
Priority to JP56213193A priority Critical patent/JPS58110600A/ja
Priority to DE8282111895T priority patent/DE3277307D1/de
Priority to US06/452,290 priority patent/US4686191A/en
Priority to CA000418366A priority patent/CA1211059A/en
Priority to EP82111895A priority patent/EP0083069B1/en
Publication of JPS58110600A publication Critical patent/JPS58110600A/ja
Publication of JPH0134599B2 publication Critical patent/JPH0134599B2/ja
Granted legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/555—Interferons [IFN]
    • C07K14/565—IFN-beta
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C12N15/71—Expression systems using regulatory sequences derived from the trp-operon
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S930/00—Peptide or protein sequence
    • Y10S930/01—Peptide or protein sequence
    • Y10S930/14—Lymphokine; related peptides
    • Y10S930/142—Interferon

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
JP56213193A 1981-12-25 1981-12-25 ヒトβ型インタ−フエロン遺伝子を含む組みかえ体プラスミド Granted JPS58110600A (ja)

Priority Applications (5)

Application Number Priority Date Filing Date Title
JP56213193A JPS58110600A (ja) 1981-12-25 1981-12-25 ヒトβ型インタ−フエロン遺伝子を含む組みかえ体プラスミド
DE8282111895T DE3277307D1 (en) 1981-12-25 1982-12-22 Recombinant plasmid containing human interferon-beta gene
US06/452,290 US4686191A (en) 1981-12-25 1982-12-22 Recombinant plasmid containing human interferon-beta gene
CA000418366A CA1211059A (en) 1981-12-25 1982-12-22 RECOMBINANT PLASMID CONTAINING HUMAN INTERFERON-.beta. GENE
EP82111895A EP0083069B1 (en) 1981-12-25 1982-12-22 Recombinant plasmid containing human interferon-beta gene

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP56213193A JPS58110600A (ja) 1981-12-25 1981-12-25 ヒトβ型インタ−フエロン遺伝子を含む組みかえ体プラスミド

Publications (2)

Publication Number Publication Date
JPS58110600A JPS58110600A (ja) 1983-07-01
JPH0134599B2 true JPH0134599B2 (https=) 1989-07-20

Family

ID=16635072

Family Applications (1)

Application Number Title Priority Date Filing Date
JP56213193A Granted JPS58110600A (ja) 1981-12-25 1981-12-25 ヒトβ型インタ−フエロン遺伝子を含む組みかえ体プラスミド

Country Status (5)

Country Link
US (1) US4686191A (https=)
EP (1) EP0083069B1 (https=)
JP (1) JPS58110600A (https=)
CA (1) CA1211059A (https=)
DE (1) DE3277307D1 (https=)

Families Citing this family (98)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS58141796A (ja) * 1982-02-18 1983-08-23 Kyowa Hakko Kogyo Co Ltd ペプチドの製造法
EP0121569B1 (en) * 1982-10-08 1990-08-22 Kyowa Hakko Kogyo Co., Ltd. Novel vector
JPH0740925B2 (ja) * 1983-03-14 1995-05-10 協和醗酵工業株式会社 新規ヒトインタ−フエロン−γポリペプチド
JPS59203495A (ja) * 1983-04-28 1984-11-17 Kyowa Hakko Kogyo Co Ltd 外来遺伝子の新規な発現方法
EP0152483B1 (en) * 1983-08-22 1989-03-29 Kyowa Hakko Kogyo Co., Ltd. Process for producing peptide
JPS60188077A (ja) * 1984-03-09 1985-09-25 Teruhiko Beppu 仔牛プロキモシンｃＤΝＡの全配列を有する新規な発現プラスミド
EP0165613B1 (en) * 1984-06-22 1992-01-15 Hitachi, Ltd. Process for controlling culture of recombinants
FR2579224B1 (fr) * 1985-03-25 1987-05-22 Genetica Procede de preparation microbiologique de la serum-albumine humaine
DK156072C (da) * 1985-04-03 1989-11-06 Nordisk Gentofte Syntetisk dna-sekvens indeholdende et ribosombindingssted
DK174044B1 (da) 1986-12-23 2002-05-06 Kyowa Hakko Kogyo Kk Polypeptid afledt fra human granulocytkolonistimulerende faktor, og fremgangsmåde til fremstilling deraf, DNA kodende for nævnte polypeptid, rekombinant plasmid indeholdende nævnte DNA, og mikroorganismer indeholdende nævnte rekombinante plasmid.......
DE4128319A1 (de) * 1991-08-27 1993-03-04 Bioferon Biochem Substanz Neues rekombinantes human-ifn-beta, verfahren zu dessen herstellung und dieses enthaltende pharmazeutische zubereitungen
US5723125A (en) * 1995-12-28 1998-03-03 Tanox Biosystems, Inc. Hybrid with interferon-alpha and an immunoglobulin Fc linked through a non-immunogenic peptide
EP2175025A1 (en) 1998-04-14 2010-04-14 Kyowa Hakko Bio Co., Ltd. Process for producing isoprenoid compounds by using microorganisms
AU3956399A (en) 1998-06-02 1999-12-20 Kyowa Hakko Kogyo Co. Ltd. Iga nephropathy-associated dna
US7144574B2 (en) 1999-08-27 2006-12-05 Maxygen Aps Interferon β variants and conjugates
US6531122B1 (en) * 1999-08-27 2003-03-11 Maxygen Aps Interferon-β variants and conjugates
US7431921B2 (en) 1999-08-27 2008-10-07 Maxygen Aps Interferon beta-like molecules
WO2001098493A1 (en) 2000-06-15 2001-12-27 Kyowa Hakko Kogyo Co., Ltd Insulin-like growth factor-binding protein
FR2810674A1 (fr) * 2000-06-22 2001-12-28 Pf Medicament Construction modifiee en amont du codon d'initiation pour la surexpression de proteines recombinantes
US20020169290A1 (en) * 2000-11-02 2002-11-14 Claus Bornaes New multimeric interferon beta polypeptides
JP3765574B2 (ja) * 2001-02-22 2006-04-12 三菱化学株式会社 逆向き反復配列を含む組み換え遺伝子及びその利用
EP2080771A3 (en) 2001-02-27 2010-01-06 Maxygen Aps New interferon beta-like molecules
US20040117868A1 (en) * 2002-01-29 2004-06-17 Jun Imamura Protein participating in restoration from cytoplasmic male sterility to fertility and gene encoding the same
AU2002251533B2 (en) 2001-04-25 2006-09-28 Institut National De La Recherche Agronomique Protein involved in restoration of cytoplasmic male sterility to fertility and gene encoding the protein and gene
US6995236B2 (en) 2001-05-08 2006-02-07 Riken Sphingomyelin detecting probe
US7402418B2 (en) * 2001-09-20 2008-07-22 Plantech Research Institute Genes participating in the synthesis of fatty acid having trans-11-,cis-13-conjugated double bond and utilization thereof
JP2003144141A (ja) * 2001-11-14 2003-05-20 Gencom Co ＲＮＡｉ効果が増強したＥＳ細胞
PT1502956E (pt) 2002-05-08 2009-08-31 Kyowa Hakko Bio Co Ltd Processo de produção de citidina 5'-difosfato colina
JP4171256B2 (ja) * 2002-07-18 2008-10-22 三菱化学株式会社 パピローマウイルスベクターを用いたＲＮＡｉ表現型を有する非ヒト哺乳動物の作製方法
US20060248617A1 (en) 2002-08-30 2006-11-02 Japan Science And Technology Corporation Method of targeted gene disruption, genome of hyperthermostable bacterium and genome chip using the same
JP2004180641A (ja) * 2002-12-06 2004-07-02 Gencom Co ＲＮＡｉ表現型を有する非ヒト哺乳動物の作製方法
CN100400660C (zh) 2002-12-26 2008-07-09 协和发酵工业株式会社 生产二肽的方法
EP1602720B1 (en) * 2003-03-05 2010-11-03 Juridical Foundation The Chemo-Sero-Therapeutic Research Institute Process for producing heterologous protein in e. coli
EP2327724A3 (en) 2004-02-02 2011-07-27 Ambrx, Inc. Modified human growth hormone polypeptides and their uses
US20080076729A1 (en) * 2005-05-19 2008-03-27 Schering Aktiengesellachaft Interferon-beta gene therapy using an improved, regulated expression system
AR053284A1 (es) * 2005-05-19 2007-04-25 Schering Ag Terapia genica de interferon -beta usando un sistema mejorado de expresion regulada
CN101238214A (zh) * 2005-05-19 2008-08-06 拜尔先灵制药股份公司 使用改进的调节表达系统治疗疾病
US20070179113A1 (en) * 2005-05-19 2007-08-02 Schering Aktiengesellachaft GM-CSF gene therapy for Crohn's disease using an improved regulated expression system
US20090270314A1 (en) 2005-09-29 2009-10-29 Tohoku University Polypeptide having anti-angiogenic activity
US8058037B2 (en) 2005-11-29 2011-11-15 Kyowa Hakko Bio Co., Ltd. Protein and DNA encoding the protein
CA2632419A1 (en) 2005-12-06 2007-06-14 Kyowa Hakko Kogyo Co., Ltd. Genetically recombinant anti-perp antibody
JP5592059B2 (ja) 2005-12-27 2014-09-17 協和発酵バイオ株式会社 Ｌ−グルタミンの製造法
JP5586146B2 (ja) 2006-06-06 2014-09-10 協和発酵キリン株式会社 ヘパリン結合上皮細胞増殖因子様増殖因子に結合するモノクローナル抗体
EP2067857B1 (en) 2006-09-25 2012-04-11 Kyowa Hakko Bio Co., Ltd Method for production of dipeptide
EP2123751A4 (en) 2007-01-25 2010-06-02 Kyowa Hakko Kirin Co Ltd NEW PEPTIDE
WO2008114733A1 (ja) 2007-03-16 2008-09-25 Kyowa Hakko Kirin Co., Ltd. 抗Claudin-4抗体
EP2140876A4 (en) 2007-03-29 2011-08-03 Univ Okayama Nat Univ Corp THERAPEUTIC AGENT WITH VASOHIBIN
WO2008126784A1 (ja) 2007-04-06 2008-10-23 Kyowa Hakko Bio Co., Ltd. グルタチオンおよびγ-グルタミルシステインの製造法
ES2526490T3 (es) 2007-04-06 2015-01-12 Kyowa Hakko Bio Co., Ltd. Método para producir un dipéptido
MX2009011870A (es) * 2007-05-02 2009-11-12 Ambrx Inc Polipeptidos de interferon beta modificados y usos de los mismos.
JP5550041B2 (ja) 2007-09-27 2014-07-16 塩野義製薬株式会社 チトクロームｐ４５０を用いたアダマンタン水酸化体の製造方法
JP5532401B2 (ja) 2007-12-05 2014-06-25 協和発酵キリン株式会社 ヘパリン結合上皮細胞増殖因子様増殖因子に結合するモノクローナル抗体
KR20110039220A (ko) 2008-06-30 2011-04-15 교와 핫꼬 기린 가부시키가이샤 항ｃｄ27 항체
US8268592B2 (en) 2008-07-17 2012-09-18 Kyowa Hakko Kirin Co., Ltd Anti-system ASC amino acid transporter 2 (ASCT2) antibody
KR20110069138A (ko) 2008-10-09 2011-06-22 교와 메덱스 가부시키가이샤 신규 프럭토실펩티드옥시다아제
WO2010074266A1 (ja) 2008-12-26 2010-07-01 協和発酵キリン株式会社 抗cd4抗体
EP2395096B1 (en) 2009-02-09 2014-04-09 Kyowa Hakko Bio Co., Ltd. Process for producing L-amino acids
CA2759146C (en) 2009-04-20 2017-06-13 Kyowa Kirin Co., Ltd. Agonist anti-cd40 antibody
WO2011155607A1 (ja) 2010-06-11 2011-12-15 協和発酵キリン株式会社 抗ｔｉｍ－３抗体
JP5058332B2 (ja) 2010-07-14 2012-10-24 住友ゴム工業株式会社 イソプレンオリゴマー、ポリイソプレン、及びこれらの製造方法、ゴム組成物、並びに空気入りタイヤ
AU2012274461A1 (en) 2011-06-20 2014-01-16 Kyowa Hakko Kirin Co., Ltd. Anti-erbB3 antibody
CN104411720B (zh) 2012-07-02 2018-05-11 协和发酵麒麟株式会社 以抗bmp9抗体作为有效成分的、对肾性贫血、癌性贫血等贫血的治疗剂
JP6224619B2 (ja) 2012-12-07 2017-11-01 協和発酵キリン株式会社 抗ｆｏｌｒ１抗体
JP6328115B2 (ja) 2013-07-09 2018-05-23 協和メデックス株式会社 糖化ヘキサペプチドオキシダーゼとその利用
CN115011572A (zh) 2016-03-04 2022-09-06 国立大学法人新潟大学 变异型四异戊烯基-β-姜黄烯环化酶及龙涎香醇的制备方法
WO2018021301A1 (ja) 2016-07-26 2018-02-01 静岡県 抗ｂ７－ｈ４抗体
JP6842720B2 (ja) 2016-08-10 2021-03-17 Ｓｐｉｂｅｒ株式会社 不溶性組換えタンパク質凝集体の製造方法
CN109843905A (zh) 2016-10-03 2019-06-04 丝芭博株式会社 重组蛋白的纯化方法
EP3611253A4 (en) 2017-04-13 2020-12-23 Kyowa Hakko Bio Co., Ltd. THEANINE PRODUCTION PROCESS
US11091545B2 (en) 2017-07-13 2021-08-17 Kyowa Kirin Co., Ltd. Methods of using an antibody to stabilize and analyze a BRIL fusion protein
TWI910093B (zh) 2017-07-18 2026-01-01 日商協和麒麟股份有限公司 抗人類ｃｃｒ１單株抗體與其製造方法及其用途、產生抗人類ｃｃｒ１單株抗體之細胞、編碼抗人類ｃｃｒ１單株抗體之核酸及包含該核酸之轉形細胞、人類ｃｃｒ１之檢測或測定用試劑、人類ｃｃｒ１相關疾病之診斷及治療藥、以及抗人類ｃｃｒ１單株抗體之衍生物
US11692015B2 (en) 2017-07-26 2023-07-04 Spiber Inc. Modified fibroin
CN111094571B (zh) 2017-09-01 2023-10-27 国立大学法人新潟大学 龙涎香醇的有效制备方法
KR102782136B1 (ko) 2017-11-08 2025-03-18 쿄와 기린 가부시키가이샤 CD40과 EpCAM에 결합하는 이중특이적 항체
CA3085563A1 (en) 2017-12-12 2019-06-20 Kyowa Kirin Co., Ltd. Anti-bmp10 antibody, and therapeutic agent for hypertension and hypertensive diseases comprising said antibody as an active ingredient
CN112424357B (zh) 2018-06-26 2024-12-24 协和麒麟株式会社 与硫酸软骨素蛋白聚糖5结合的抗体
TWI857959B (zh) 2018-06-26 2024-10-11 日商協和麒麟股份有限公司 與細胞黏著分子3 結合之抗體
US12241101B2 (en) 2018-08-10 2025-03-04 Kyowa Hakko Bio Co., Ltd. Microorganism producing polyunsaturated fatty acid and method for producing polyunsaturated fatty acid
BR112021002300A2 (pt) 2018-08-10 2021-05-04 Kyowa Hakko Bio Co., Ltd microrganismo que produz ácido eicosapentaenoico e método para a produção de ácido eicosapentaenoico
JP7506607B2 (ja) 2018-12-28 2024-06-26 協和キリン株式会社 ＴｆＲに結合するバイスペシフィック抗体
JPWO2020145363A1 (ja) 2019-01-09 2021-11-25 Ｓｐｉｂｅｒ株式会社 改変フィブロイン
BR112021014805A2 (pt) 2019-01-31 2022-01-04 Spiber Inc Métodos de produção para uma proteína recombinante e para aumentar o volume de produção
WO2020230899A1 (ja) 2019-05-15 2020-11-19 協和キリン株式会社 Ｃｄ４０とｆａｐに結合するバイスペシフィック抗体
WO2020230901A1 (ja) 2019-05-15 2020-11-19 協和キリン株式会社 Ｃｄ４０とｇｐｃ３に結合するバイスペシフィック抗体
WO2021125245A1 (ja) 2019-12-16 2021-06-24 協和発酵バイオ株式会社 改変されたラクトースパーミアーゼを有する微生物及びラクトース含有オリゴ糖の製造法
TWI888487B (zh) 2020-02-14 2025-07-01 日商協和麒麟股份有限公司 與ｃｄ３結合之雙特異性抗體
JPWO2021187502A1 (https=) 2020-03-16 2021-09-23
US20240141402A1 (en) 2020-06-25 2024-05-02 Kyowa Hakko Bio Co., Ltd. Method for producing dipeptide
CN116848248A (zh) 2021-02-08 2023-10-03 协和发酵生化株式会社 具有含有岩藻糖的糖质的转运活性的蛋白和含有岩藻糖的糖质的制造方法
CA3229748A1 (en) 2021-08-26 2023-03-02 Akifumi Kato Bispecific antibody that binds to cd116 and cd131
KR20240043794A (ko) 2021-09-10 2024-04-03 교와 핫꼬 바이오 가부시키가이샤 Cdp-콜린의 제조에 이용하는 재조합 미생물 및 상기 재조합 미생물을 이용하는 cdp-콜린의 제조 방법
CN118679260A (zh) 2022-02-09 2024-09-20 国立研究开发法人医药基盘·健康·营养研究所 与fcrl1结合的抗体或该抗体片段
CN118984876A (zh) 2022-04-28 2024-11-19 协和发酵生化株式会社 具有nampt活性的蛋白质和nmn的制造方法
AU2023284238A1 (en) 2022-06-06 2024-12-19 Plumino Precision Fermentation Japan Co., Ltd. Microorganism having improved 3’-sialyllactose productivity and production method of 3’-sialyllactose
US20260002181A1 (en) 2022-06-30 2026-01-01 Kirin Holdings Kabushiki Kaisha Method for producing 4-(aminomethyl)cyclohexane-1-carboxylic acid
JP7820529B2 (ja) 2022-08-10 2026-02-25 協和キリン株式会社 抗ｆｇｆ２３抗体又は該抗体断片
JPWO2024122636A1 (https=) 2022-12-09 2024-06-13
EP4726047A1 (en) 2023-06-29 2026-04-15 Kirin Holdings Kabushiki Kaisha Method for producing 4-(aminomethyl)cyclohexane-1-carboxylic acid

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IE52036B1 (en) * 1979-05-24 1987-05-27 Univ California Non-passageable viruses
AU542264B2 (en) * 1979-06-01 1985-02-14 G.D. Searle & Co. Plasmid vectors
ZA811368B (en) * 1980-03-24 1982-04-28 Genentech Inc Bacterial polypedtide expression employing tryptophan promoter-operator
FI88175C (fi) * 1980-04-03 1993-04-13 Biogen Inc Rekombinant-dna-molekyler och foerfaranden foer framstaellning av polypeptider liknande humant -interferon
NZ198445A (en) * 1980-09-25 1984-05-31 Genentech Inc Production of human fibroblast interferon by recombinant dna technology

Also Published As

Publication number Publication date
EP0083069B1 (en) 1987-09-16
DE3277307D1 (en) 1987-10-22
EP0083069A2 (en) 1983-07-06
EP0083069A3 (en) 1984-03-28
CA1211059A (en) 1986-09-09
JPS58110600A (ja) 1983-07-01
US4686191A (en) 1987-08-11

Similar Documents

Publication Publication Date Title
JPH0134599B2 (https=)
US4673640A (en) Regulated protein production using site-specific recombination
US5122457A (en) Expression systems utilizing bacteriophage t7 promoters, gene sequences, and t7 rna polymerase
EP0198015B2 (en) Production of streptavidin-like polypeptides
JP2887248B2 (ja) ポリペプチドの製造方法
CA1198068A (en) Bacterial polypeptide expression employing tryptophan promoter-operator
IL173399A0 (en) Circular dna molecule with conditional origin of replication which contains nucleic acid of interest and selection marker but does not encode its replication initiation protein, method for preparing the same and use thereof in gene therapy
JPH0669375B2 (ja) 特異的切断リンカ−
JPH0665305B2 (ja) 組換えｄｎａ含有宿主細胞の安定化法
KR890014738A (ko) 단백질 합성의 cks 방법
JP3837154B2 (ja) 遺伝子発現用プロモーター
US4716112A (en) Vectors for increased expression of cloned genes
US4795706A (en) Novel expression control sequences
Rosenberg et al. T7 RNA polymerase can direct expression of influenza virus cap-binding protein (PB2) in Escherichia coli
GB2024229A (en) Process for producing proteins by the expression of the corresponding ADN in bacteria, modified DNA and vectors applicable in such processes
Trojanowska et al. The bacteriophage T4 regA gene: primary sequence of a translational repressor
JPH0829099B2 (ja) 組換ｄｎａ、発現ベクター及びプラスミド、これらの製法及び誘発可能で抑制可能な外来遺伝子の発現法
WO1985004419A1 (en) High copy number expression vectors
EP0314184B1 (en) Expression plasmids
KR830007827A (ko) 신규 재조합 플라스미드의 제조방법
JP2570651B2 (ja) Ｍｅｔ−インスリン様成長因子Ｉの製造方法
JPH02100685A (ja) ポリペプチド分泌発現ベクター，該ベクターで形質転換した微生物及び該微生物によるポリペプチドの製造
DE3882465D1 (de) Expression von mutarotase.
Fraser The future of recombinant DNA technology in medicine
JPH0817708B2 (ja) Ｍｅｔ−インスリン様成長因子Ｉの発現ベクター