JP7161291B2 - 変異体ポア - Google Patents

変異体ポア Download PDF

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Publication number
JP7161291B2
JP7161291B2 JP2017512039A JP2017512039A JP7161291B2 JP 7161291 B2 JP7161291 B2 JP 7161291B2 JP 2017512039 A JP2017512039 A JP 2017512039A JP 2017512039 A JP2017512039 A JP 2017512039A JP 7161291 B2 JP7161291 B2 JP 7161291B2
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JP
Japan
Prior art keywords
csgg
pore
polynucleotide
seq
monomers
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JP2017512039A
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English (en)
Japanese (ja)
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JP2017527284A5 (enExample
JP2017527284A (ja
Inventor
ホウォルカ，ステファン
リモート，ハン
ジャヤシンゲ，ラクマル
エリザベス，ジェーン ウォレス
アンソニー クラーク，ジェームス
ジョージ ハンブリー，リチャード
バンクス ピュー，ジョナサン
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Vlaams Instituut voor Biotechnologie VIB
Vrije Universiteit Brussel VUB
Original Assignee
Vlaams Instituut voor Biotechnologie VIB
Vrije Universiteit Brussel VUB
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Priority claimed from GB201415455A external-priority patent/GB201415455D0/en
Priority claimed from GBGB1506754.9A external-priority patent/GB201506754D0/en
Priority claimed from GBGB1508287.8A external-priority patent/GB201508287D0/en
Priority claimed from GBGB1511203.0A external-priority patent/GB201511203D0/en
Priority claimed from GBGB1515240.8A external-priority patent/GB201515240D0/en
Application filed by Vlaams Instituut voor Biotechnologie VIB, Vrije Universiteit Brussel VUB filed Critical Vlaams Instituut voor Biotechnologie VIB
Publication of JP2017527284A publication Critical patent/JP2017527284A/ja
Publication of JP2017527284A5 publication Critical patent/JP2017527284A5/ja
Priority to JP2022133867A priority Critical patent/JP2022177005A/ja
Publication of JP7161291B2 publication Critical patent/JP7161291B2/ja
Application granted granted Critical
Priority to JP2024085524A priority patent/JP2024122996A/ja
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/245—Escherichia (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/22—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a Strep-tag
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/116—Nucleic acid detection characterized by the use of physical, structural and functional properties electrical properties of nucleic acids, e.g. impedance, conductivity or resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/60—Detection means characterised by use of a special device
    • C12Q2565/631—Detection means characterised by use of a special device being a biochannel or pore

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Electrochemistry (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Enzymes And Modification Thereof (AREA)
JP2017512039A 2014-09-01 2015-09-01 変異体ポア Active JP7161291B2 (ja)

Priority Applications (2)

Application Number Priority Date Filing Date Title
JP2022133867A JP2022177005A (ja) 2014-09-01 2022-08-25 変異体ポア
JP2024085524A JP2024122996A (ja) 2014-09-01 2024-05-27 変異体ポア

Applications Claiming Priority (15)

Application Number Priority Date Filing Date Title
GB1415455.3 2014-09-01
GB201415455A GB201415455D0 (en) 2014-09-01 2014-09-01 Protein pores and sensing applications
GB201422079 2014-12-11
GB1422079.2 2014-12-11
GB1506489.2 2015-04-16
GBGB1506489.2A GB201506489D0 (en) 2014-09-01 2015-04-16 Protein pores for sensing applications
GB1506754.9 2015-04-21
GBGB1506754.9A GB201506754D0 (en) 2015-04-21 2015-04-21 Mutant pore
GB1508287.8 2015-05-14
GBGB1508287.8A GB201508287D0 (en) 2015-05-14 2015-05-14 Mutant pores
GB1511203.0 2015-06-25
GBGB1511203.0A GB201511203D0 (en) 2015-06-25 2015-06-25 Mutant pore
GBGB1515240.8A GB201515240D0 (en) 2015-08-27 2015-08-27 Mutant pore
GB1515240.8 2015-08-27
PCT/EP2015/069965 WO2016034591A2 (en) 2014-09-01 2015-09-01 Mutant pores

Related Child Applications (2)

Application Number Title Priority Date Filing Date
JP2020204136A Division JP2021078500A (ja) 2014-09-01 2020-12-09 変異体ポア
JP2022133867A Division JP2022177005A (ja) 2014-09-01 2022-08-25 変異体ポア

Publications (3)

Publication Number Publication Date
JP2017527284A JP2017527284A (ja) 2017-09-21
JP2017527284A5 JP2017527284A5 (enExample) 2018-10-11
JP7161291B2 true JP7161291B2 (ja) 2022-10-26

Family

ID=55440449

Family Applications (4)

Application Number Title Priority Date Filing Date
JP2017512039A Active JP7161291B2 (ja) 2014-09-01 2015-09-01 変異体ポア
JP2020204136A Pending JP2021078500A (ja) 2014-09-01 2020-12-09 変異体ポア
JP2022133867A Pending JP2022177005A (ja) 2014-09-01 2022-08-25 変異体ポア
JP2024085524A Pending JP2024122996A (ja) 2014-09-01 2024-05-27 変異体ポア

Family Applications After (3)

Application Number Title Priority Date Filing Date
JP2020204136A Pending JP2021078500A (ja) 2014-09-01 2020-12-09 変異体ポア
JP2022133867A Pending JP2022177005A (ja) 2014-09-01 2022-08-25 変異体ポア
JP2024085524A Pending JP2024122996A (ja) 2014-09-01 2024-05-27 変異体ポア

Country Status (8)

Country Link
US (3) US10400014B2 (enExample)
EP (2) EP3224274B1 (enExample)
JP (4) JP7161291B2 (enExample)
KR (3) KR20250130441A (enExample)
CN (7) CN117164682A (enExample)
AU (1) AU2015310913B2 (enExample)
CA (1) CA2959220A1 (enExample)
WO (1) WO2016034591A2 (enExample)

Families Citing this family (127)

* Cited by examiner, † Cited by third party
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Ｅｐｓｔｅｉｎ， Ｅｌｉｓａｂｅｔｈ Ａｓｈｍａｎ， "Ａｓｓｅｍｂｌｙ， Ｓｐａｔｉａｌ Ｄｉｓｔｒｉｂｕｔｉｏｎ， ａｎｄ Ｓｅｃｒｅｔｉｏｎ Ａｃｔｉｖｉｔｙ ｏｆ ｔｈｅ Ｃｕｒｌｉｎ Ｓｅｃｒｅｔｉｏｎ Ｌｉｐｏｐｒｏｔｅｉｎ， ＣｓｇＧ．"， Ｄｅｅｐ Ｂｌｕｅ Ｒｅｐｏｓｉｔｏｒｉｅｓ［ｏｎｌｉｎｅ］， ２００８年， Ｔｈｅ Ｕｎｉｖｅｒｓｉｔｙ ｏｆ Ｍｉｃｈｉｇａｎ， Ａｎｎ Ａｒｂｏｒ， ＭＩ， ＵＳＡ．［ｒｅｔｒｉｅｖｅｄ ｏｎ １ Ｊｕｎｅ ２０２２］， Ｒｅｔｒｉｅｖｅｄ ｆｒｏｍ ｔｈｅ Ｉｎｔｅｒｎｅｔ：＜ＵＲＬ：ｈｔｔｐｓ：／／ｄｅｅｐｂｌｕｅ．ｌｉｂ．ｕｍｉｃｈ．ｅｄｕ／ｂｉｔｓｔｒｅａｍ／ｈａｎｄｌｅ／２０２７．４２／６１６６７／ｅｍａｓｈｍａｎ＿１．ｐｄｆ＞， ＩＳＢＮ： ９７８０５４９９８４８５６
ＰＮＡＳ， ２０１４年１２月１日， Ｖｏｌ．１１１， Ｎｏ．５０， ｐｐ．Ｅ５４３９－Ｅ５４４４

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