JP6276918B2 - 多能性幹細胞を未分化状態で培養する培地、細胞培養および方法 - Google Patents

多能性幹細胞を未分化状態で培養する培地、細胞培養および方法 Download PDF

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JP6276918B2
JP6276918B2 JP2012538468A JP2012538468A JP6276918B2 JP 6276918 B2 JP6276918 B2 JP 6276918B2 JP 2012538468 A JP2012538468 A JP 2012538468A JP 2012538468 A JP2012538468 A JP 2012538468A JP 6276918 B2 JP6276918 B2 JP 6276918B2
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ミカル アミット，
ミカル アミット，
ヨセフ イツコヴィッツ−エルドー，
ヨセフ イツコヴィッツ−エルドー，
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Technion Research and Development Foundation Ltd
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JP2012538468A 2009-11-12 2010-11-11 多能性幹細胞を未分化状態で培養する培地、細胞培養および方法 Active JP6276918B2 (ja)

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US27286009P 2009-11-12 2009-11-12
US61/272,860 2009-11-12
PCT/IL2010/000937 WO2011058558A2 (en) 2009-11-12 2010-11-11 Culture media, cell cultures and methods of culturing pluripotent stem cells in an undifferentiated state

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JP2013510567A JP2013510567A (ja) 2013-03-28
JP2013510567A5 JP2013510567A5 (enExample) 2013-12-26
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JP2012538468A Active JP6276918B2 (ja) 2009-11-12 2010-11-11 多能性幹細胞を未分化状態で培養する培地、細胞培養および方法
JP2015207773A Pending JP2016047056A (ja) 2009-11-12 2015-10-22 多能性幹細胞を未分化状態で培養する培地、細胞培養および方法
JP2017152419A Active JP6603694B2 (ja) 2009-11-12 2017-08-07 多能性幹細胞を未分化状態で培養する培地、細胞培養および方法
JP2019188087A Active JP7053551B2 (ja) 2009-11-12 2019-10-11 多能性幹細胞を未分化状態で培養する培地、細胞培養および方法
JP2022059325A Active JP7383069B2 (ja) 2009-11-12 2022-03-31 多能性幹細胞を未分化状態で培養する培地、細胞培養および方法

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JP2017152419A Active JP6603694B2 (ja) 2009-11-12 2017-08-07 多能性幹細胞を未分化状態で培養する培地、細胞培養および方法
JP2019188087A Active JP7053551B2 (ja) 2009-11-12 2019-10-11 多能性幹細胞を未分化状態で培養する培地、細胞培養および方法
JP2022059325A Active JP7383069B2 (ja) 2009-11-12 2022-03-31 多能性幹細胞を未分化状態で培養する培地、細胞培養および方法

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EP (3) EP2499236B1 (enExample)
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CA (3) CA2783437C (enExample)
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Families Citing this family (46)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2499236B1 (en) 2009-11-12 2020-01-01 Technion Research & Development Foundation Ltd. Culture media, cell cultures and methods of culturing pluripotent stem cells in an undifferentiated state
US9969981B2 (en) 2010-03-01 2018-05-15 Janssen Biotech, Inc. Methods for purifying cells derived from pluripotent stem cells
US9279107B2 (en) 2010-08-05 2016-03-08 Wisconsin Alumni Research Foundation Simplified basic media for human pluripotent cell culture
KR101829488B1 (ko) * 2010-08-05 2018-02-14 위스콘신 얼럼나이 리서어치 화운데이션 인간 다분화능 세포 배양을 위한 단순화된 기본 배지
US10214722B2 (en) 2010-09-07 2019-02-26 Technion Research & Development Foundation Limited Methods for expanding and maintaining human pluripotent stem cells (PSCs) in an undifferentiated state in a single cell suspension culture
CN106893692B (zh) 2010-12-22 2021-11-26 菲特治疗公司 用于单细胞分选与增强ipsc重新编程的细胞培养平台
EP4086338A1 (en) 2011-03-17 2022-11-09 Minerva Biotechnologies Corporation Method for making pluripotent stem cells
GB201117469D0 (en) * 2011-10-10 2011-11-23 Cell Guidance Systems Ltd Culture media for pluripotent stem cells
WO2013059373A2 (en) 2011-10-17 2013-04-25 Minerva Biotechnologies Corporation Media for stem cell proliferation and induction
WO2013077423A1 (ja) 2011-11-25 2013-05-30 国立大学法人京都大学 多能性幹細胞の培養方法
DK3260140T3 (da) 2011-12-05 2021-04-19 Factor Bioscience Inc Fremgangsmåder og produkter til transficering af celler
US8497124B2 (en) 2011-12-05 2013-07-30 Factor Bioscience Inc. Methods and products for reprogramming cells to a less differentiated state
AU2012355698B2 (en) 2011-12-22 2018-11-29 Janssen Biotech, Inc. Differentiation of human embryonic stem cells into single hormonal insulin positive cells
AR090970A1 (es) * 2012-05-07 2014-12-17 Janssen Biotech Inc Diferenciacion de celulas madre humanas embrionarias en endodermo pancreatico
MX2015008577A (es) 2012-12-31 2015-09-07 Janssen Biotech Inc Cultivo de celulas madre embrionarias humanas en la interfase aire-liquido para la diferenciacion en celulas endocrinas pancreaticas.
EP4039798A1 (en) 2012-12-31 2022-08-10 Janssen Biotech, Inc. Suspension and clustering of human pluripotent cells
US10370644B2 (en) 2012-12-31 2019-08-06 Janssen Biotech, Inc. Method for making human pluripotent suspension cultures and cells derived therefrom
WO2014136581A1 (ja) 2013-03-06 2014-09-12 国立大学法人京都大学 多能性幹細胞の培養システム及び多能性幹細胞の継代方法
CA3135208A1 (en) 2013-06-11 2014-12-18 Ncardia B.V. Culture medium compositions for maturating cardiomyocytes derived from pluripotent mammalian stem cells
WO2014208295A1 (ja) * 2013-06-28 2014-12-31 株式会社カネカ 多能性幹細胞の増殖促進因子のスクリーニング法
JP6174155B2 (ja) * 2013-09-10 2017-08-02 富士フイルム株式会社 多能性幹細胞の培養方法ならびにこれに用いる多能性幹細胞の培養用キットおよび培地
WO2015037622A1 (ja) 2013-09-10 2015-03-19 富士フイルム株式会社 多能性幹細胞の培養方法、培養用キット及び多能性幹細胞培養用培地
JP6516280B2 (ja) * 2013-12-26 2019-05-22 国立大学法人広島大学 ｉＰＳ細胞の樹立方法および幹細胞の長期維持方法
WO2015098111A1 (ja) * 2013-12-26 2015-07-02 国立大学法人広島大学 ｉＰＳ細胞の樹立方法および幹細胞の長期維持方法
SG10201807292YA (en) * 2014-03-04 2018-09-27 Fate Therapeutics Inc Improved reprogramming methods and cell culture platforms
EP3140395B1 (en) * 2014-05-06 2020-01-22 F. Hoffmann-La Roche AG Method for differentiation of pluripotent stem cells into cardiomyocytes
US20150335010A1 (en) 2014-05-21 2015-11-26 Bourbon Corporation Maintenance medium for primate pluripotent stem cells
CN104388382B (zh) * 2014-11-21 2017-05-10 吉林大学 利用搅拌式细胞培养转瓶机快速制备饲养层细胞的方法
US9944894B2 (en) 2015-01-16 2018-04-17 General Electric Company Pluripotent stem cell expansion and passage using a rocking platform bioreactor
CA2996582A1 (en) * 2015-08-31 2017-03-09 I Peace, Inc. Pluripotent stem cell manufacturing system and method for producing induced pluripotent stem cells
ES2971677T3 (es) * 2015-09-08 2024-06-06 Sumitomo Pharma Co Ltd Método para la producción de tejido retiniano
AU2016338680B2 (en) 2015-10-16 2022-11-17 Fate Therapeutics, Inc. Platform for the induction and maintenance of ground state pluripotency
KR102711870B1 (ko) * 2016-07-19 2024-10-02 아셀라타 리미티드 현탁 내 만능줄기세포를 배양하기 위한 배양 배지
WO2018029656A2 (en) * 2016-08-12 2018-02-15 The University Of Chicago Methods for making and using therapeutic exosomes
CN110621323B (zh) * 2017-05-02 2022-10-14 田边刚士 医药品组合物及化妆品组合物
WO2019181999A1 (ja) 2018-03-20 2019-09-26 富士フイルム株式会社 多能性幹細胞を三次元培養する方法
US20210087525A1 (en) * 2019-09-19 2021-03-25 Northwestern University Cost effective culture media and protocol for human induced pluripotent stem cells
JP7742836B2 (ja) * 2019-12-09 2025-09-22 デウン ファーマシューティカル カンパニー リミテッド ヒト多能性幹細胞から間葉系幹細胞の製造方法およびこれによって製造された間葉系幹細胞
JP7753883B2 (ja) * 2020-01-14 2025-10-15 味の素株式会社 細胞培養用培地組成物
CN111484970B (zh) * 2020-04-30 2022-09-16 广州再生医学与健康广东省实验室 一种低蛋白含量的无血清无饲养层胚胎与多能干细胞培养基
CA3221435A1 (en) * 2021-06-09 2022-12-15 Michal Amit Serum free media for suspension culture of mammalian livestock pluripotent stem cells
US11735303B2 (en) 2021-06-22 2023-08-22 David Haase Apparatus and method for determining a composition of a replacement therapy treatment
CN113403262A (zh) * 2021-06-23 2021-09-17 昆明理工大学 一种对食蟹猴干细胞的无饲养层培养方法
WO2023053220A1 (ja) * 2021-09-28 2023-04-06 公益財団法人京都大学ｉＰＳ細胞研究財団 多能性幹細胞の製造方法
WO2024030501A2 (en) * 2022-08-02 2024-02-08 Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College Bovine trophoblast stem cells and uses thereof
USD1069165S1 (en) 2022-11-02 2025-04-01 Abs Global, Inc. Dish holder

Family Cites Families (192)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NL154600B (nl) 1971-02-10 1977-09-15 Organon Nv Werkwijze voor het aantonen en bepalen van specifiek bindende eiwitten en hun corresponderende bindbare stoffen.
NL154598B (nl) 1970-11-10 1977-09-15 Organon Nv Werkwijze voor het aantonen en bepalen van laagmoleculire verbindingen en van eiwitten die deze verbindingen specifiek kunnen binden, alsmede testverpakking.
NL154599B (nl) 1970-12-28 1977-09-15 Organon Nv Werkwijze voor het aantonen en bepalen van specifiek bindende eiwitten en hun corresponderende bindbare stoffen, alsmede testverpakking.
US3901654A (en) 1971-06-21 1975-08-26 Biological Developments Receptor assays of biologically active compounds employing biologically specific receptors
US3853987A (en) 1971-09-01 1974-12-10 W Dreyer Immunological reagent and radioimmuno assay
US3867517A (en) 1971-12-21 1975-02-18 Abbott Lab Direct radioimmunoassay for antigens and their antibodies
NL171930C (nl) 1972-05-11 1983-06-01 Akzo Nv Werkwijze voor het aantonen en bepalen van haptenen, alsmede testverpakkingen.
US3850578A (en) 1973-03-12 1974-11-26 H Mcconnell Process for assaying for biologically active molecules
US3935074A (en) 1973-12-17 1976-01-27 Syva Company Antibody steric hindrance immunoassay with two antibodies
US3996345A (en) 1974-08-12 1976-12-07 Syva Company Fluorescence quenching with immunological pairs in immunoassays
US4034074A (en) 1974-09-19 1977-07-05 The Board Of Trustees Of Leland Stanford Junior University Universal reagent 2-site immunoradiometric assay using labelled anti (IgG)
US3984533A (en) 1975-11-13 1976-10-05 General Electric Company Electrophoretic method of detecting antigen-antibody reaction
US4098876A (en) 1976-10-26 1978-07-04 Corning Glass Works Reverse sandwich immunoassay
US4879219A (en) 1980-09-19 1989-11-07 General Hospital Corporation Immunoassay utilizing monoclonal high affinity IgM antibodies
IL68218A (en) 1983-03-23 1985-12-31 Univ Ramot Compositions for cartilage repair comprising embryonal chondrocytes
US5011771A (en) 1984-04-12 1991-04-30 The General Hospital Corporation Multiepitopic immunometric assay
US4666828A (en) 1984-08-15 1987-05-19 The General Hospital Corporation Test for Huntington's disease
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4801531A (en) 1985-04-17 1989-01-31 Biotechnology Research Partners, Ltd. Apo AI/CIII genomic polymorphisms predictive of atherosclerosis
US5272057A (en) 1988-10-14 1993-12-21 Georgetown University Method of detecting a predisposition to cancer by the use of restriction fragment length polymorphism of the gene for human poly (ADP-ribose) polymerase
US5192659A (en) 1989-08-25 1993-03-09 Genetype Ag Intron sequence analysis method for detection of adjacent and remote locus alleles as haplotypes
US5281521A (en) 1992-07-20 1994-01-25 The Trustees Of The University Of Pennsylvania Modified avidin-biotin technique
US5489304A (en) 1994-04-19 1996-02-06 Brigham & Women's Hospital Method of skin regeneration using a collagen-glycosaminoglycan matrix and cultured epithelial autograft
US5843780A (en) 1995-01-20 1998-12-01 Wisconsin Alumni Research Foundation Primate embryonic stem cells
AU7243100A (en) 1995-02-02 2001-05-03 Tristem Trading (Cyprus) Limited A method of preparing an undifferentiated cell
US7410773B2 (en) 1995-02-02 2008-08-12 Ghazi Jaswinder Dhoot Method of preparing an undifferentiated cell
JPH08308561A (ja) * 1995-05-16 1996-11-26 Sumitomo Electric Ind Ltd 動物細胞培養用無血清培地
US5811297A (en) 1996-03-07 1998-09-22 Amba Biosciences, Llc Immortalized hematopoietic cell lines, cell system thereof with stromal cells, in vitro, ex vivo and in vivo uses, & in vitro generation of dendritic cells and macrophages
JP4307552B2 (ja) 1996-03-15 2009-08-05 ミューニン コーポレイション 細胞外マトリックスシグナリング分子
US6878544B2 (en) 1996-04-19 2005-04-12 Neurotech Sa Retinal cell lines with extended life-span and their applications
EP0986635A4 (en) 1997-01-10 2001-11-07 Life Technologies Inc SERUM SUBSTITUTE FOR EMBRYONIC STEM CELLS
US6331406B1 (en) 1997-03-31 2001-12-18 The John Hopkins University School Of Medicine Human enbryonic germ cell and methods of use
US6090622A (en) 1997-03-31 2000-07-18 The Johns Hopkins School Of Medicine Human embryonic pluripotent germ cells
AU8284198A (en) 1997-07-02 1999-01-25 Board Of Regents, The University Of Texas System P53 as a regulator of cell differentiation
IL122818A0 (en) 1997-07-10 1998-08-16 Yeda Res & Dev Chimeric interleukin-6 soluble receptor/ligand protein analogs thereof and uses thereof
EP1025204A4 (en) 1997-10-23 2001-02-28 Geron Corp Methods and materials for the growth of primate-derived primordial stem cells
US7413904B2 (en) 1998-10-23 2008-08-19 Geron Corporation Human embryonic stem cells having genetic modifications
US6667176B1 (en) 2000-01-11 2003-12-23 Geron Corporation cDNA libraries reflecting gene expression during growth and differentiation of human pluripotent stem cells
US7410798B2 (en) 2001-01-10 2008-08-12 Geron Corporation Culture system for rapid expansion of human embryonic stem cells
CA2348687A1 (en) * 1998-11-09 2000-05-18 Consorzio Per La Gestione Del Centro Di Biotecnologie Avanzate Serum free medium for chondrocyte-like cells
EP1129176A4 (en) 1998-11-09 2002-10-30 Es Cell Int Pte Ltd EMBRYONIC STEM CELLS
US20030129745A1 (en) 1999-10-28 2003-07-10 Robl James M. Gynogenetic or androgenetic production of pluripotent cells and cell lines, and use thereof to produce differentiated cells and tissues
US7455983B2 (en) 2000-01-11 2008-11-25 Geron Corporation Medium for growing human embryonic stem cells
JP2001229797A (ja) 2000-02-15 2001-08-24 Yazaki Corp ガス式電源遮断装置
EP2453010A1 (en) 2000-03-14 2012-05-16 ES Cell International Pte Ltd. Neural progenitor cells derived from embryonic stem cells
EP1268835B1 (en) 2000-03-30 2008-01-16 Oxford Biomedica (UK) Limited Retinoic acid receptor beta-2 and gene therapy vectors for the treatment of neurological disorders
US7473555B2 (en) 2000-04-27 2009-01-06 Geron Corporation Protocols for making hepatocytes from embryonic stem cells
NL1017973C2 (nl) 2000-05-10 2002-11-08 Tristem Trading Cyprus Ltd Inrichting.
US7250294B2 (en) 2000-05-17 2007-07-31 Geron Corporation Screening small molecule drugs using neural cells differentiated from human embryonic stem cells
AU2001263199B2 (en) 2000-05-17 2004-09-16 Asterias Biotherapeutics, Inc. Neural progenitor cell populations
WO2001096532A2 (en) 2000-06-15 2001-12-20 Tanja Dominko Method of generating pluripotent mammalian cells by fusion of a cytoplast fragment with a karyoplast
GB0025088D0 (en) 2000-10-13 2000-11-29 Univ Edinburgh Stem cells
US6576464B2 (en) 2000-11-27 2003-06-10 Geron Corporation Methods for providing differentiated stem cells
CA2430653A1 (en) 2000-11-27 2002-08-08 Yissum Research Development Company Of The Hebrew University In Jerusalem Transfection of human embryonic stem cells
US20020168763A1 (en) 2000-11-30 2002-11-14 Yan Wen Liang Isolated homozygous stem cells, differentiated cells derived therefrom, and materials and methods for making and using same
EP1393066A4 (en) 2001-05-15 2006-01-25 Rappaport Family Inst For Res INSULIN-PRODUCING CELLS DERIVED FROM HUMAN EMBRYONIC STEM CELLS
US20030021771A1 (en) 2001-07-06 2003-01-30 Chunhui Xu Osteoblast precursors from human embryonic stem cells
IL159580A0 (en) 2001-07-12 2004-06-01 Geron Corp Cells of the cardiomyocyte lineage produced from human pluripotent stem cells
AU2002317039B2 (en) 2001-07-24 2007-10-04 Es Cell International Pte Ltd Methods of inducing differentiation of stem cells
US7432104B2 (en) 2001-08-06 2008-10-07 Bresgen Inc. Methods for the culture of human embryonic stem cells on human feeder cells
US20030211603A1 (en) 2001-08-14 2003-11-13 Earp David J. Reprogramming cells for enhanced differentiation capacity using pluripotent stem cells
US20030044976A1 (en) 2001-08-27 2003-03-06 Advanced Cell Technology De-differentiation and re-differentiation of somatic cells and production of cells for cell therapies
JP2005502356A (ja) 2001-09-12 2005-01-27 デヴェロゲン アクチエンゲゼルシャフト フュア エントヴィックルングスビオローギッシェ フォルシュング 治療のための腸内幹細胞の分離、培養および分化方法
US20050095708A1 (en) 2001-11-09 2005-05-05 Pera Martin F. Characterization and isolation of subsets of human embryonic stem cells (HES) and cells associated or derived therefrom
EP1463803B1 (en) 2001-12-07 2018-02-14 Asterias Biotherapeutics, Inc. Hematopoietic cells from human embryonic stem cells
GB2399823B (en) 2001-12-07 2006-02-15 Geron Corp Islet cells from primate pluripotent stem cells
CA2468335C (en) 2001-12-07 2014-07-08 Geron Corporation Chondrocyte precursors derived from human embryonic stem cells
CN100526455C (zh) 2001-12-21 2009-08-12 斯路姆-X股份有限公司 用于体外分离和培养具有种系传递能力的胚胎干细胞(es)细胞系的组合物
GB2398795A (en) 2001-12-28 2004-09-01 Cellartis Ab A method for the establishment of a pluripotent human blastocyst-derived stem cell line
AU2009213101B2 (en) 2002-06-05 2012-03-08 Es Cell International Pte Ltd Generation of neural stem cells from undifferentiated human embryonic stem cells
AU2003247514A1 (en) 2002-06-11 2003-12-22 Roy Ogle Meningeal-derived stem cells
US7662625B2 (en) 2002-07-02 2010-02-16 Cancer Research Technology Limited Methods for detecting the differentiation status of cells using 5T4 antigen expression
US20050101014A1 (en) 2002-07-11 2005-05-12 Keirstead Hans S. Oligodendrocytes derived from human embryonic stem cells for remyelination and treatment of spinal cord injury
US7285415B2 (en) 2002-07-11 2007-10-23 The Regents Of The University Of California Oligodendrocytes derived from human embryonic stem cells for remyelination and treatment of spinal cord injury
US7267981B2 (en) 2002-10-07 2007-09-11 Technion Research & Development Foundation Ltd. Human foreskin fibroblasts for culturing ES cells
US20060252150A1 (en) 2002-11-08 2006-11-09 Linzhao Cheng Human embryonic stem cell cultures, and compositions and methods for growing same
US20040110286A1 (en) 2002-12-06 2004-06-10 The John P. Robarts Research Institute Method for making hematopoietic cells
EP2457999B1 (en) 2002-12-16 2018-10-17 Technion Research & Development Foundation Ltd. Culture medium for pluripotent stem cells
WO2004078944A2 (en) 2003-03-05 2004-09-16 Maine Medical Center Research Institute Thrombin, soluble jaggedi, and trap and growth of stem cells
US20060134636A1 (en) 2003-03-13 2006-06-22 Stanton Lawrence W Standardization of growth conditions for human embryonic stem cells intended for use in regenerative medicine
US7153650B2 (en) 2003-03-13 2006-12-26 Geron Corporation Marker system for preparing and characterizing high-quality human embryonic stem cells
US20030224411A1 (en) 2003-03-13 2003-12-04 Stanton Lawrence W. Genes that are up- or down-regulated during differentiation of human embryonic stem cells
US20070053890A1 (en) 2003-03-28 2007-03-08 Sunnybrook And Women's College Health Sciences Centre Canine embryonic stem cells
WO2004099394A2 (en) 2003-05-08 2004-11-18 Cellartis Ab A method for efficient transfer of human blastocyst-derived stem cells (hbs cells) from a feeder-supported to a feeder-free culture system
US20050118713A1 (en) 2003-05-12 2005-06-02 Nikolai Strelchenko Morula derived embryonic stem cells
US20040229350A1 (en) 2003-05-12 2004-11-18 Nikolai Strelchenko Morula derived embryonic stem cells
US20050042595A1 (en) 2003-08-14 2005-02-24 Martin Haas Banking of multipotent amniotic fetal stem cells
CA2549158A1 (en) 2003-12-01 2005-06-16 Technion Research And Development Foundation Ltd. Methods of generating stem cells and embryonic bodies carrying disease-causing mutations and methods of using same for studying genetic disorders
TWI280280B (en) 2003-12-09 2007-05-01 Ind Tech Res Inst Culture system and method for expansion and undifferentiated growth of human embryonic stem cells
WO2005065354A2 (en) 2003-12-31 2005-07-21 The Burnham Institute Defined media for pluripotent stem cell culture
WO2005080551A2 (en) 2004-02-12 2005-09-01 University Of Newcastle Upon Tyne Stem cells
US20060216821A1 (en) 2004-02-26 2006-09-28 Reliance Life Sciences Pvt. Ltd. Pluripotent embryonic-like stem cells derived from corneal limbus, methods of isolation and uses thereof
EP1730261A4 (en) 2004-03-10 2007-11-28 Univ California COMPOSITIONS AND METHODS FOR GROWING EMBRYONIC STEM CELLS
IL178006A (en) 2004-03-19 2011-08-31 Geron Corp Method for making high purity cardiomyocyte preparations suitable for regenerative medicine
EP1725654B1 (en) 2004-03-19 2019-05-01 Asterias Biotherapeutics, Inc. Method for making high purity cardiomyocyte preparations suitable for regenerative medicine
JP4688793B2 (ja) 2004-03-23 2011-05-25 敏宏 赤池 多能性幹細胞の増殖方法
US7452718B2 (en) 2004-03-26 2008-11-18 Geron Corporation Direct differentiation method for making cardiomyocytes from human embryonic stem cells
US20050214938A1 (en) 2004-03-26 2005-09-29 Gold Joseph D Cardiac bodies: clusters of spontaneously contracting cells for regenerating cardiac function
US20050227352A1 (en) 2004-04-09 2005-10-13 Stowers Institute For Medical Research Use of BMP (BAM) signaling to control stem cell fate determination
US9375514B2 (en) 2004-05-21 2016-06-28 Fraunhofer-Gesellschaft Zur Forderung Der Angewandten Forschung E.V. Multicellular tissue and organ culture systems
JP5128946B2 (ja) * 2004-05-21 2013-01-23 ウィセル リサーチ インスティテュート インコーポレイテッド 胚性幹細胞のフィーダー非依存性長期培養
US20080038820A1 (en) 2004-06-22 2008-02-14 Rudy-Reil Diane E Induction of pluripotent stem cells into mesodermal lineages
US20090136559A1 (en) 2004-07-09 2009-05-28 Athanasiou Kyriacos A Chondrocyte Differentiation from Human Embryonic Stem Cells and Their Use in Tissue Engineering
AU2005271723B2 (en) 2004-07-13 2010-12-16 Asterias Biotherapeutics, Inc. Medium for growing human embryonic stem cells
CA2504451A1 (en) 2004-08-10 2006-02-10 Geron Corporation Dendritic cell vaccines for treating cancer made from embryonic stem cells
WO2006017855A2 (en) 2004-08-12 2006-02-16 Board Of Regents Of The University Of Texas System Methods and compositions for regulation of stem cell survival, proliferation, and differentiation by protein ubiquitination
DE102004043256B4 (de) 2004-09-07 2013-09-19 Rheinische Friedrich-Wilhelms-Universität Bonn Skalierbarer Prozess zur Kultivierung undifferenzierter Stammzellen in Suspension
US7442548B2 (en) 2004-09-08 2008-10-28 Wisconsin Alumni Research Foundation Culturing human embryonic stem cells in medium containing pipecholic acid and gamma amino butyric acid
CA2580754A1 (en) 2004-09-21 2006-03-30 Nvr Labs, Ltd. Compositions and methods for stem cell expansion and differentiation
EP1799810A2 (en) 2004-10-12 2007-06-27 Technion Research & Development Foundation Ltd. Isolated primate embryonic cells and methods of generating and using same
JP4646600B2 (ja) * 2004-11-02 2011-03-09 オリンパス株式会社 間葉系幹細胞の培養方法
US7641897B2 (en) 2004-11-23 2010-01-05 The Board Of Trustees Of The Leland Stanford Junior University Feeder layer and serum independent embryonic stem cells
FR2878860A1 (fr) 2004-12-08 2006-06-09 Vivalis Sa Lignees de cellules souches humaines derivees de cellules es et utilisations pour la production de vaccins et de proteines recombinantes
WO2006070370A2 (en) 2004-12-29 2006-07-06 Hadasit Medical Research Services & Development Limited Stem cells culture systems
US8597947B2 (en) 2004-12-29 2013-12-03 Hadasit Medical Research Services & Development Limited Undifferentiated stem cell culture systems
JP2008538276A (ja) 2005-01-28 2008-10-23 ノヴァセラ・リミテッド 胚幹細胞培養のための方法
US20060182724A1 (en) 2005-02-15 2006-08-17 Riordan Neil H Method for expansion of stem cells
CN101189329A (zh) 2005-02-23 2008-05-28 新加坡科技研究局 无限繁殖化饲养细胞
US8012751B2 (en) 2005-03-31 2011-09-06 Wisconsin Alumni Research Foundation Differentiation of pluripotent embryonic stem cells
US7972767B2 (en) 2005-05-09 2011-07-05 Olympus Corporation Method for culturing mesenchymal stem cell and method for producing biological tissue prosthesis
JP4755460B2 (ja) * 2005-07-27 2011-08-24 オリンパス株式会社 間葉系幹細胞の培養方法
US9446073B2 (en) 2005-05-15 2016-09-20 Biodontos, Llc Non-lineage committed precursor cells from the dental papillary tissue of teeth
US20110311977A1 (en) 2005-05-17 2011-12-22 Arundhati Mandal In Vitro Generation of Hepatocytes from Human Embryonic Stem Cells
EP1893748A1 (en) 2005-05-17 2008-03-05 Reliance Life Sciences Pvt., Ltd. Establishment of a human embryonic stem cell line using mammalian cells
WO2007002210A2 (en) 2005-06-20 2007-01-04 Bresagen, Inc. Embryonic stem cell culture compositions and methods of use thereof
AU2006262369B2 (en) 2005-06-22 2012-07-05 Asterias Biotherapeutics, Inc. Suspension culture of human embryonic stem cells
JP2009502124A (ja) * 2005-07-15 2009-01-29 プライムジェン バイオテック エルエルシー 生殖系列幹細胞の療法的再プログラミング
EP1962719A4 (en) 2005-08-29 2011-05-04 Technion Res And Dev Of Foundation Ltd MEDIA FOR BREEDING STEM CELLS
US7754483B2 (en) 2005-11-09 2010-07-13 The Penn State Research Foundation Systems and methods for selection and maintenance of homogeneous and pluripotent human embryonic stem cells
US20080070303A1 (en) 2005-11-21 2008-03-20 West Michael D Methods to accelerate the isolation of novel cell strains from pluripotent stem cells and cells obtained thereby
KR101420740B1 (ko) 2005-12-13 2014-07-17 교또 다이가꾸 핵초기화 인자
US20070249044A1 (en) 2006-01-30 2007-10-25 University Of Illinois At Chicago Microstructures in three dimensional gel suspensions for growth of cells
KR101348325B1 (ko) 2006-01-31 2014-01-08 각고호우징 게이오기주크 줄기세포 및 태아에서 유래된 심근세포 및 예정 심근세포의제조방법
EP1994141B1 (en) 2006-02-23 2017-11-15 ViaCyte, Inc. Compositions and methods useful for culturing differentiable cells
JP2007228815A (ja) 2006-02-27 2007-09-13 Gifu Univ 胚性幹細胞の維持方法
WO2007122233A1 (en) 2006-04-25 2007-11-01 Vrije Universiteit Brussel Preparation of mesenchymal progenitor cells, particularly osteogenic progenitor cells
US8241908B2 (en) 2006-05-16 2012-08-14 Becton, Dickinson And Company Extracellular matrix coated surface for culturing cells
US9096831B2 (en) 2006-05-19 2015-08-04 Versitech Limited Methods to enhance cell migration and engraftment
GB0613756D0 (en) * 2006-07-12 2006-08-23 Univ Sheffield Cell culture medium
DK3441459T3 (da) 2006-08-02 2021-06-07 Technion Res & Dev Foundation Fremgangsmåder til ekspansion af embryonale stamceller i en suspensionskultur
WO2008033469A1 (en) 2006-09-15 2008-03-20 Children's Medical Center Corporation Methods for producing embryonic stem cells from parthenogenetic embryos
WO2008054819A2 (en) 2006-11-02 2008-05-08 The General Hospital Corporation Cardiovascular stem cells, methods for stem cell isolation, and uses thereof
DK2081956T3 (da) 2006-11-13 2013-06-24 Charite Universitaetsmedizin FREMGANGSMÅDE TIL CELLEDYRKNING OG FREMGANGSMÅDE TIL BEHANDLING OMFATTENDE EN vEPO-PROTEINVARIANT
US8414928B2 (en) 2006-12-06 2013-04-09 George R. Dodge Methods for the generation of cartilage-like material by mechanical loading
WO2008075378A2 (en) 2006-12-19 2008-06-26 Stempeutics Research Private Limited Xeno-free culture conditions for human embryonic stem cells and methods thereof
US20080182328A1 (en) 2006-12-19 2008-07-31 The Burnham Institute Mammalian extraembryonic endoderm cells and methods of isolation
DK2137295T3 (en) 2007-01-04 2018-06-25 Karl Tryggvason Use of a composition and method for enabling proliferation of pluripotent stem cells
US10829733B2 (en) 2007-01-04 2020-11-10 Biolamina Ab Composition and method for enabling proliferation of pluripotent human stem cells
JP5419279B2 (ja) 2007-01-17 2014-02-19 ウィスコンシン アラムニ リサーチ ファンデーション 改良された幹細胞の培養
JP2010516239A (ja) 2007-01-18 2010-05-20 スオメン プナイネン リスティ，ヴェリパルベル 細胞の産生に対する新規方法および試薬
US20110033928A1 (en) 2007-03-09 2011-02-10 The Regents Of The University Of Michigan Methods and compositions for growth of cells and embryonic tissue on a synthetic polymer matrix
DE102007020302B4 (de) 2007-04-20 2012-03-22 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Verbesserte dreidimensionale biokompatible Gerüststruktur, die Nanopartikel beinhaltet
US20090053182A1 (en) 2007-05-25 2009-02-26 Medistem Laboratories, Inc. Endometrial stem cells and methods of making and using same
FI20075417A0 (fi) * 2007-06-05 2007-06-05 Marjo-Riitta Suuronen Koostumuksia ja menetelmiä alkion kantasolujen kasvatukseen
JP2008307007A (ja) 2007-06-15 2008-12-25 Bayer Schering Pharma Ag 出生後のヒト組織由来未分化幹細胞から誘導したヒト多能性幹細胞
KR20100059789A (ko) 2007-06-29 2010-06-04 셀룰러 다이내믹스 인터내셔널, 인코포레이티드 배아줄기세포 배양을 위한 자동화 방법 및 장치
EP2028268A1 (en) 2007-08-20 2009-02-25 Université Libre De Bruxelles Generation of neuronal cells from pluripotent stem cells
WO2009026474A2 (en) 2007-08-21 2009-02-26 The Salk Institute For Biological Studies Robust regression based exon array protocol system and applications
US8623650B2 (en) 2007-10-19 2014-01-07 Viacyte, Inc. Methods and compositions for feeder-free pluripotent stem cell media containing human serum
EP2072618A1 (en) 2007-12-14 2009-06-24 Johannes Gutenberg-Universität Mainz Use of RNA for reprogramming somatic cells
AU2008338989A1 (en) 2007-12-17 2009-06-25 Gliamed, Inc. Stem-like cells and method for reprogramming adult mammalian somatic cells
WO2009099555A2 (en) 2008-01-30 2009-08-13 Corning Incorporated Synthetic surfaces for culturing cells in chemically defined media
SG188098A1 (en) * 2008-01-30 2013-03-28 Geron Corp Synthetic surfaces for culturing stem cell derived cardiomyocytes
CN102232109A (zh) 2008-05-27 2011-11-02 奥尔胡斯大学 用于哺乳动物干细胞生长和分化的生物相容性材料
US20100120142A1 (en) 2008-07-11 2010-05-13 Suomen Punainen Risti Veripalvelu Culture of human embryonic cells
US20100035327A1 (en) 2008-08-11 2010-02-11 Ann Marie Steele Use of rice-derived products in a universal cell culture medium
SG160248A1 (en) 2008-09-18 2010-04-29 Agency Science Tech & Res Use of novel monoclonal antibodies targeting human embryonic stem cells to characterize and kill induced pluripotent stem cells
US8008075B2 (en) 2008-11-04 2011-08-30 Viacyte, Inc. Stem cell aggregate suspension compositions and methods of differentiation thereof
US8895300B2 (en) 2008-11-04 2014-11-25 Viacyte, Inc. Scalable primate pluripotent stem cell aggregate suspension culture and differentiation thereof
US8956867B2 (en) 2008-11-07 2015-02-17 Wisconsin Alumni Research Foundation Method for culturing stem cells
KR101720961B1 (ko) 2009-02-27 2017-03-29 셀룰러 다이내믹스 인터내셔널, 인코포레이티드 다능성 세포의 분화
SG10201401864VA (en) 2009-04-27 2014-09-26 Viacyte Inc Small Molecules Supporting Pluripotent Cell Growth And Methods Thereof
WO2010134619A1 (ja) 2009-05-18 2010-11-25 国立大学法人東北大学 人工多能性幹細胞からの上皮系前駆細胞・幹細胞群及び角膜上皮細胞群の分化誘導方法
EA026732B1 (ru) 2009-06-11 2017-05-31 Минерва Байотекнолоджиз Корпорейшн Способы культивирования стволовых клеток и клеток-предшественников
WO2010144059A2 (en) 2009-06-12 2010-12-16 Nanyang Polytechnic Novel uses
KR101166257B1 (ko) 2009-06-16 2012-07-19 한국생명공학연구원 미분화 다능성줄기세포의 제작, 유지 및 증식을 위한 뉴로펩타이드 ｙ를 포함하는 배지 조성물 및 이를 이용한 다능성줄기세포의 배양 방법
WO2011019953A1 (en) 2009-08-12 2011-02-17 University Of Southern California Defined conditions for human embryonic stem cell culture and passage
CA2777552C (en) 2009-10-19 2019-05-21 Cellular Dynamics International, Inc. Cardiomyocyte production
EP2499236B1 (en) 2009-11-12 2020-01-01 Technion Research & Development Foundation Ltd. Culture media, cell cultures and methods of culturing pluripotent stem cells in an undifferentiated state
WO2011063005A2 (en) 2009-11-17 2011-05-26 Advanced Cell Technology, Inc. Methods of producing human rpe cells and pharmaceutical preparations of human rpe cells
WO2011075686A2 (en) 2009-12-18 2011-06-23 Ventria Bioscience Methods & compositions comprising heat shock proteins
CA2784847C (en) 2009-12-18 2017-11-21 Molly Sandra Shoichet Injectable polymer composition for use as a cell delivery vehicle
US20140030236A1 (en) 2010-02-25 2014-01-30 The Johns Hopkins University Derivation and maturation of synthetic and contractile vascular smooth muscle cells from human pluripotent stem cells
WO2011110886A1 (en) 2010-03-09 2011-09-15 Biolamina Ab Composition and method for enabling proliferation of pluripotent human stem cells
US20120100110A1 (en) 2010-04-20 2012-04-26 International Stem Cell Corporation Physiological methods for isolation of high purity cell populations
WO2011140397A2 (en) 2010-05-05 2011-11-10 The Regents Of The University Of California Office Of The President Stem cell defined media for xeno-free and feeder free conditions and uses thereof
US11786636B2 (en) 2010-06-15 2023-10-17 Versitech Limited Methods for complex tissue engineering
KR101861171B1 (ko) 2010-06-18 2018-05-31 후지필름 셀룰러 다이내믹스, 인코포레이티드 투석된 혈청이 있는 심근세포 배지
ES2874953T3 (es) 2010-07-23 2021-11-05 Astellas Inst For Regenerative Medicine Métodos para la detección de subpoblaciones raras de células y composiciones muy purificadas de células
US20120141432A1 (en) 2010-09-03 2012-06-07 University Of Pittsburgh- Of The Commonwealth System Of Higher Education Use of catalytic antioxidant to preserve stem cell phenotype and control cell differentiation
US10214722B2 (en) 2010-09-07 2019-02-26 Technion Research & Development Foundation Limited Methods for expanding and maintaining human pluripotent stem cells (PSCs) in an undifferentiated state in a single cell suspension culture
WO2012034101A2 (en) 2010-09-09 2012-03-15 The Regents Of The University Of California Expandable cell source of neuronal stem cell populations and methods for obtaining and using them

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