JP6043354B2 - 熱安定性が向上したサイコースエピマー化酵素変異体及びこれを用いたサイコースの連続的生産 - Google Patents
熱安定性が向上したサイコースエピマー化酵素変異体及びこれを用いたサイコースの連続的生産 Download PDFInfo
- Publication number
- JP6043354B2 JP6043354B2 JP2014527066A JP2014527066A JP6043354B2 JP 6043354 B2 JP6043354 B2 JP 6043354B2 JP 2014527066 A JP2014527066 A JP 2014527066A JP 2014527066 A JP2014527066 A JP 2014527066A JP 6043354 B2 JP6043354 B2 JP 6043354B2
- Authority
- JP
- Japan
- Prior art keywords
- cyclose
- enzyme
- mutant
- amino acid
- epimerizing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/90—Isomerases (5.)
- C12N9/92—Glucose isomerase (5.3.1.5; 5.3.1.9; 5.3.1.18)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/24—Preparation of compounds containing saccharide radicals produced by the action of an isomerase, e.g. fructose
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/90—Isomerases (5.)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N15/77—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Corynebacterium; for Brevibacterium
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/02—Monosaccharides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y501/00—Racemaces and epimerases (5.1)
- C12Y501/03—Racemaces and epimerases (5.1) acting on carbohydrates and derivatives (5.1.3)
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR10-2011-0084712 | 2011-08-24 | ||
| KR1020110084712A KR101203856B1 (ko) | 2011-08-24 | 2011-08-24 | 열 안정성이 향상된 사이코스 에피머화 효소 변이체 및 이를 이용한 사이코스의 연속적 생산 |
| PCT/KR2012/006637 WO2013027999A2 (ko) | 2011-08-24 | 2012-08-21 | 열 안정성이 향상된 사이코스 에피머화 효소 변이체 및 이를 이용한 사이코스의 연속적 생산 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2014525244A JP2014525244A (ja) | 2014-09-29 |
| JP6043354B2 true JP6043354B2 (ja) | 2016-12-14 |
Family
ID=47565145
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2014527066A Active JP6043354B2 (ja) | 2011-08-24 | 2012-08-21 | 熱安定性が向上したサイコースエピマー化酵素変異体及びこれを用いたサイコースの連続的生産 |
Country Status (15)
| Country | Link |
|---|---|
| US (1) | US9217166B2 (enExample) |
| EP (2) | EP2749645B1 (enExample) |
| JP (1) | JP6043354B2 (enExample) |
| KR (1) | KR101203856B1 (enExample) |
| CN (1) | CN104160023B (enExample) |
| DK (1) | DK2749645T3 (enExample) |
| ES (1) | ES2626501T3 (enExample) |
| HR (1) | HRP20170813T1 (enExample) |
| HU (1) | HUE035316T2 (enExample) |
| IN (1) | IN2014CN01614A (enExample) |
| PL (1) | PL2749645T3 (enExample) |
| PT (1) | PT2749645T (enExample) |
| RS (1) | RS56088B1 (enExample) |
| SI (1) | SI2749645T1 (enExample) |
| WO (1) | WO2013027999A2 (enExample) |
Families Citing this family (35)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101318422B1 (ko) * | 2013-04-09 | 2013-10-15 | 주식회사 삼양제넥스 | D-사이코스 에피머화 효소, 및 이를 이용하는 사이코스 생산방법 |
| KR101455624B1 (ko) | 2013-04-12 | 2014-10-28 | 주식회사한국야쿠르트 | 기능성 희귀당 사이코스의 생산능을 지닌 신규 클로스트리디움 볼티에 유래의 사이코스-3-에피머화 효소 및 이를 이용한 사이코스 생산방법 |
| KR101455759B1 (ko) | 2013-04-23 | 2014-10-28 | 씨제이제일제당(주) | 사이코스 에피머화 효소 변이체 및 이를 이용한 사이코스의 제조 방법 |
| EP2843044A1 (en) * | 2013-09-03 | 2015-03-04 | Roquette Frères | Improved variant of D-psicose 3-epimerase and uses thereof |
| WO2015032761A1 (en) * | 2013-09-03 | 2015-03-12 | Roquette Freres | Improved variant of d-psicose 3-epimerase and uses thereof |
| KR101539097B1 (ko) | 2013-12-26 | 2015-07-23 | 주식회사 삼양제넥스 | 사이코스 에피머화 효소를 암호화 하는 폴리뉴클레오타이드, 및 이를 이용하는 사이코스 생산방법 |
| KR101473918B1 (ko) | 2014-05-28 | 2014-12-17 | 대상 주식회사 | 사이코스 에피머화 효소, 이의 제조방법 및 이를 이용한 사이코스의 제조방법 |
| KR101577147B1 (ko) * | 2014-10-01 | 2015-12-11 | 경상대학교산학협력단 | 사이코스의 생산 방법 |
| KR101607633B1 (ko) | 2014-10-21 | 2016-04-11 | 주식회사 삼양사 | 코리네박테리움속 균주를 이용한 사이코스 생산방법 |
| JP6563013B2 (ja) | 2014-10-30 | 2019-08-21 | サムヤン コーポレイション | プシコースエピメラーゼの発現システムおよびこれを用いたプシコースの生産 |
| US10266862B2 (en) | 2014-11-06 | 2019-04-23 | Industry-Academic Cooperation Foundation Gyeongsang National University | Method for preparing psicose |
| KR102044957B1 (ko) * | 2014-12-31 | 2019-11-14 | 주식회사 삼양사 | 사이코스의 제조방법 |
| KR101677368B1 (ko) * | 2015-04-02 | 2016-11-18 | 씨제이제일제당 (주) | 신규 프로모터 및 이의 용도 |
| KR102087396B1 (ko) | 2015-11-16 | 2020-03-10 | 주식회사 삼양사 | 과당-함유 기질로부터 사이코스를 생산하는 방법 |
| WO2017111563A1 (ko) | 2015-12-23 | 2017-06-29 | 씨제이제일제당(주) | D-사이코스 3-에피머화 효소 및 염을 포함하는 d-사이코스 제조용 조성물 및 이를 이용한 d-사이코스의 제조 방법 |
| KR101723007B1 (ko) | 2016-02-29 | 2017-04-04 | 씨제이제일제당(주) | 고순도 d-사이코스를 제조하는 방법 |
| CN105821027B (zh) | 2016-04-01 | 2023-11-21 | 南京朗奈生物技术有限公司 | 一种3-差向异构酶的用途 |
| KR101946157B1 (ko) * | 2016-07-29 | 2019-02-11 | 씨제이제일제당 (주) | D-타가토스 전환 활성이 향상된 헥수론산 c4-에피머화 효소 변이체 및 이를 이용한 d-타가토스의 제조 방법 |
| CN106148311B (zh) * | 2016-09-12 | 2019-06-21 | 上海立足生物科技有限公司 | 一种d-阿洛酮糖-3-差向异构酶的突变体及其应用 |
| KR101919713B1 (ko) * | 2016-11-16 | 2018-11-19 | 씨제이제일제당 (주) | 신규한 d-사이코스 3-에피머화 효소 및 이를 이용한 d-사이코스의 제조 방법 |
| CN108239633B (zh) * | 2016-12-26 | 2023-05-05 | 上海立足生物科技有限公司 | 一种催化活性得到提高的d-阿洛酮糖-3-差向异构酶的突变体及其应用 |
| KR20190100386A (ko) | 2017-01-06 | 2019-08-28 | 그린라이트 바이오사이언시스, 아이엔씨. | 당의 무세포 생산 |
| CA3088598C (en) | 2018-01-24 | 2021-03-30 | Matsutani Chemical Industry Co., Ltd. | Ketose 3-epimerase with improved thermal stability |
| KR102439295B1 (ko) | 2018-11-30 | 2022-09-02 | 씨제이제일제당 주식회사 | D-사이코스 결정 및 이의 제조 방법 |
| CN110396513B (zh) * | 2019-07-19 | 2022-01-11 | 天津科技大学 | 一种d-阿洛酮糖-3-差向异构酶的突变体及其应用 |
| CN110684762B (zh) * | 2019-11-12 | 2021-03-19 | 南京工业大学 | 一种d-阿洛酮糖-3-差向异构酶突变体及其编码基因、重组载体、重组菌株和应用 |
| CN111019928B (zh) * | 2019-12-11 | 2022-08-16 | 吉林中粮生化有限公司 | D-阿洛酮糖3-差向异构酶的编码基因、载体、重组细胞以及它们的应用 |
| KR102254411B1 (ko) | 2019-12-19 | 2021-05-24 | 대상 주식회사 | 알룰로스 에피머화 효소 변이체, 이의 제조방법 및 이를 이용한 알룰로스의 제조방법 |
| WO2021199445A1 (en) * | 2020-04-03 | 2021-10-07 | Rensselaer Polytechnic Institute | Method for producing sulfated polysaccharide and method for producing paps |
| KR102448351B1 (ko) | 2020-04-27 | 2022-09-28 | 대상 주식회사 | 알룰로스 에피머화 효소 변이체, 이의 제조방법 및 이를 이용한 알룰로스의 제조방법 |
| JP7348457B2 (ja) | 2020-06-03 | 2023-09-21 | ティアンゴン バイオテクノロジー（ティアンジン）カンパニー，リミテッド | プシコース３－エピメラーゼミュータント、それを発現するための遺伝子工学菌、その固定化酵素及び固定化方法 |
| CN111793616B (zh) * | 2020-08-07 | 2022-04-12 | 天津科技大学 | 一种差向异构酶的突变体及其应用 |
| KR102682846B1 (ko) * | 2021-11-19 | 2024-07-08 | 대상 주식회사 | 열 안정성이 우수한 알룰로스 에피머화 효소 변이체, 이의 제조방법 및 이를 이용한 알룰로스의 제조방법 |
| KR20240108871A (ko) * | 2022-12-30 | 2024-07-10 | 주식회사 삼양사 | 알룰로스 3-에피머화 변이 효소 및 이의 용도 |
| WO2025111274A2 (en) * | 2023-11-20 | 2025-05-30 | Novozymes A/S | Epimerase variants and polynucleotides encoding same |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100744479B1 (ko) * | 2005-06-01 | 2007-08-01 | 씨제이 주식회사 | 사이코스 에피머화 효소에 의한 사이코스의 생산 방법 |
| GB0512150D0 (en) * | 2005-06-15 | 2005-07-20 | Scottish Crop Res Inst | Production of L-ascorbic acid |
| KR20110035805A (ko) * | 2009-09-30 | 2011-04-06 | 씨제이제일제당 (주) | 사이코스-에피머화 효소의 고정화 및 이를 이용한 사이코스의 제조방법 |
-
2011
- 2011-08-24 KR KR1020110084712A patent/KR101203856B1/ko active Active
-
2012
- 2012-08-21 PL PL12826072T patent/PL2749645T3/pl unknown
- 2012-08-21 DK DK12826072.6T patent/DK2749645T3/en active
- 2012-08-21 HU HUE12826072A patent/HUE035316T2/en unknown
- 2012-08-21 JP JP2014527066A patent/JP6043354B2/ja active Active
- 2012-08-21 US US14/240,647 patent/US9217166B2/en active Active
- 2012-08-21 EP EP12826072.6A patent/EP2749645B1/en active Active
- 2012-08-21 PT PT128260726T patent/PT2749645T/pt unknown
- 2012-08-21 RS RS20170604A patent/RS56088B1/sr unknown
- 2012-08-21 IN IN1614CHN2014 patent/IN2014CN01614A/en unknown
- 2012-08-21 CN CN201280052112.0A patent/CN104160023B/zh active Active
- 2012-08-21 SI SI201230996T patent/SI2749645T1/sl unknown
- 2012-08-21 EP EP17153617.0A patent/EP3192867A1/en not_active Withdrawn
- 2012-08-21 WO PCT/KR2012/006637 patent/WO2013027999A2/ko not_active Ceased
- 2012-08-21 HR HRP20170813TT patent/HRP20170813T1/hr unknown
- 2012-08-21 ES ES12826072.6T patent/ES2626501T3/es active Active
Also Published As
| Publication number | Publication date |
|---|---|
| WO2013027999A3 (ko) | 2013-05-10 |
| ES2626501T3 (es) | 2017-07-25 |
| PL2749645T3 (pl) | 2017-09-29 |
| WO2013027999A2 (ko) | 2013-02-28 |
| KR101203856B1 (ko) | 2012-11-21 |
| WO2013027999A9 (ko) | 2013-07-11 |
| US9217166B2 (en) | 2015-12-22 |
| HRP20170813T1 (hr) | 2017-08-11 |
| EP2749645A2 (en) | 2014-07-02 |
| JP2014525244A (ja) | 2014-09-29 |
| CN104160023A (zh) | 2014-11-19 |
| EP2749645B1 (en) | 2017-04-26 |
| HUE035316T2 (en) | 2018-05-02 |
| SI2749645T1 (sl) | 2017-08-31 |
| RS56088B1 (sr) | 2017-10-31 |
| EP3192867A1 (en) | 2017-07-19 |
| IN2014CN01614A (enExample) | 2015-05-08 |
| CN104160023B (zh) | 2017-03-29 |
| US20140199732A1 (en) | 2014-07-17 |
| PT2749645T (pt) | 2017-07-28 |
| DK2749645T3 (en) | 2017-08-21 |
| EP2749645A4 (en) | 2015-06-10 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP6043354B2 (ja) | 熱安定性が向上したサイコースエピマー化酵素変異体及びこれを用いたサイコースの連続的生産 | |
| US8735106B2 (en) | Immobilization of psicose-epimerase and a method of producing D-psicose using the same | |
| JP6200578B2 (ja) | サイコースエピマー化酵素変異体及びこれを用いたサイコースの製造方法 | |
| KR100744479B1 (ko) | 사이코스 에피머화 효소에 의한 사이코스의 생산 방법 | |
| EP2540835B1 (en) | PROCESS FOR PRODUCTION OF POLY(3-HYDROXYBUTYRATE-CO-3-HYDROXYHEXANOATE) USING A GENETICALLY MODIFIED CUPRIAVIDUS NECATOR HAVING AN ENOYL-CoA HYDRATASE GENE INTRODUCED THEREIN | |
| CN104903457A (zh) | 3-差向异构酶 | |
| CN101631856A (zh) | 由棒状杆菌属表达的阿拉伯糖异构酶和使用其制造塔格糖的方法 | |
| JP2010510776A (ja) | Ｇｒａｓ微生物から発現される食品安全型好熱性のアラビノース異性化酵素及びそれを用いたタガトースの製造方法 | |
| CN114480317A (zh) | 表达乙酰乙酰辅酶a还原酶变体的工程化微生物及提高pha产量的方法 | |
| JP7784060B2 (ja) | ３－ヒドロキシアジピン酸および／またはα－ヒドロムコン酸を生産するための遺伝子改変微生物および当該化学品の製造方法 | |
| US20150344865A1 (en) | Method of Production of Rare Disaccharides | |
| US8137946B2 (en) | Recombinant GRAS strains expressing thermophilic arabinose isomerase as an active form and method of preparing food grade tagatose by using the same | |
| CN110462041A (zh) | 突变型2-脱氧-青蟹肌糖合酶 | |
| HK1175207B (en) | Immobilization of psicose-epimerase and a method of producing d-psicose using the same |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20150512 |
|
| A601 | Written request for extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A601 Effective date: 20150806 |
|
| A601 | Written request for extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A601 Effective date: 20150909 |
|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20151009 |
|
| A02 | Decision of refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A02 Effective date: 20160301 |
|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20160701 |
|
| A911 | Transfer to examiner for re-examination before appeal (zenchi) |
Free format text: JAPANESE INTERMEDIATE CODE: A911 Effective date: 20160816 |
|
| TRDD | Decision of grant or rejection written | ||
| A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 Effective date: 20161018 |
|
| A61 | First payment of annual fees (during grant procedure) |
Free format text: JAPANESE INTERMEDIATE CODE: A61 Effective date: 20161111 |
|
| R150 | Certificate of patent or registration of utility model |
Ref document number: 6043354 Country of ref document: JP Free format text: JAPANESE INTERMEDIATE CODE: R150 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |