JP5924888B2 - 核酸抽出方法、核酸抽出試薬キットおよび核酸抽出用試薬 - Google Patents

核酸抽出方法、核酸抽出試薬キットおよび核酸抽出用試薬 Download PDF

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Publication number
JP5924888B2
JP5924888B2 JP2011185275A JP2011185275A JP5924888B2 JP 5924888 B2 JP5924888 B2 JP 5924888B2 JP 2011185275 A JP2011185275 A JP 2011185275A JP 2011185275 A JP2011185275 A JP 2011185275A JP 5924888 B2 JP5924888 B2 JP 5924888B2
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Prior art keywords
nucleic acid
acid extraction
reagent
test sample
extraction reagent
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Japanese (ja)
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JP2013042750A5 (https=
JP2013042750A (ja
Inventor
卓磨 佐野
卓磨 佐野
智之 千室
智之 千室
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Kanto Chemical Co Inc
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Kanto Chemical Co Inc
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Priority to JP2011185275A priority Critical patent/JP5924888B2/ja
Priority to US13/592,390 priority patent/US20130066062A1/en
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/02—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with ribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2011185275A 2011-08-26 2011-08-26 核酸抽出方法、核酸抽出試薬キットおよび核酸抽出用試薬 Active JP5924888B2 (ja)

Priority Applications (2)

Application Number Priority Date Filing Date Title
JP2011185275A JP5924888B2 (ja) 2011-08-26 2011-08-26 核酸抽出方法、核酸抽出試薬キットおよび核酸抽出用試薬
US13/592,390 US20130066062A1 (en) 2011-08-26 2012-08-23 Nucleic acid extraction method, nucleic acid extraction reagent kit, and nucleic acid extraction reagent

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP2011185275A JP5924888B2 (ja) 2011-08-26 2011-08-26 核酸抽出方法、核酸抽出試薬キットおよび核酸抽出用試薬

Publications (3)

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JP2013042750A JP2013042750A (ja) 2013-03-04
JP2013042750A5 JP2013042750A5 (https=) 2014-09-18
JP5924888B2 true JP5924888B2 (ja) 2016-05-25

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US (1) US20130066062A1 (https=)
JP (1) JP5924888B2 (https=)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012170907A2 (en) 2011-06-08 2012-12-13 Life Technologies Corporation Polymerization of nucleic acids using proteins having low isoelectric points
FI3461807T3 (fi) 2011-06-08 2023-09-07 Life Technologies Corp Uudenlaisten detergenttien suunnittelu ja kehitys pcr-järjestelmissä käyttöä varten
EP3539944A1 (en) 2013-10-25 2019-09-18 Life Technologies Corporation Novel compounds for use in pcr systems and applications thereof
JP7143596B2 (ja) * 2017-03-13 2022-09-29 東ソー株式会社 核酸抽出および増幅試薬
CN111549024B (zh) * 2020-05-09 2023-06-30 浙江省中药研究所有限公司 一种核酸提取试纸条及其使用方法
CN113106148A (zh) * 2021-03-31 2021-07-13 湖南菲思特精准医疗科技有限公司 一种氯吡格雷剂量相关的基因多态性检测试剂盒及其检测方法和应用

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9425138D0 (en) * 1994-12-12 1995-02-08 Dynal As Isolation of nucleic acid
JP4298912B2 (ja) * 1997-07-07 2009-07-22 メディカル リサーチ カウンシル ｉｎｖｉｔｒｏ選別法
US6914137B2 (en) * 1997-12-06 2005-07-05 Dna Research Innovations Limited Isolation of nucleic acids
US7214484B2 (en) * 2002-12-17 2007-05-08 Sigma-Aldrich Co. Compositions and methods for nucleic acid extraction from biological samples
JP4228688B2 (ja) * 2002-12-25 2009-02-25 和光純薬工業株式会社 Ｄｎａ遊離方法
JP2004254525A (ja) * 2003-02-24 2004-09-16 Kubota Corp クリプトスポリジウムの種識別的検出方法
JP2006061041A (ja) * 2004-08-25 2006-03-09 Kubota Corp 核酸抽出方法及び核酸抽出キット
CN101128604A (zh) * 2005-02-28 2008-02-20 生物探索公司 用于进行涉及核酸分子的直接酶反应的方法
JP2007124926A (ja) * 2005-11-02 2007-05-24 Visionbio Corp Ｄｎａ抽出剤およびそれを用いるｄｎａ抽出方法
KR20080066727A (ko) * 2005-11-02 2008-07-16 가부시키가이샤 시마즈세이사쿠쇼 Ｒｎａ의 추출방법 및 ｒｎａ의 검출방법
JPWO2007116450A1 (ja) * 2006-03-30 2009-08-20 Ｂｉｏｃｏｓｍ株式会社 核酸抽出方法及び核酸抽出キット
CA2861667C (en) * 2007-10-01 2017-06-13 Longhorn Vaccines And Diagnostics, Llc Biological specimen collection and transport system and methods of use

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US20130066062A1 (en) 2013-03-14
JP2013042750A (ja) 2013-03-04

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