JP2575604B2 - 沈澱異種蛋白質の精製及び活性化法 - Google Patents

沈澱異種蛋白質の精製及び活性化法

Info

Publication number
JP2575604B2
JP2575604B2 JP58241820A JP24182083A JP2575604B2 JP 2575604 B2 JP2575604 B2 JP 2575604B2 JP 58241820 A JP58241820 A JP 58241820A JP 24182083 A JP24182083 A JP 24182083A JP 2575604 B2 JP2575604 B2 JP 2575604B2
Authority
JP
Japan
Prior art keywords
protein
solution
refractor
denaturing
buffer
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
JP58241820A
Other languages
English (en)
Japanese (ja)
Other versions
JPS59161321A (ja
Inventor
スチユア−ト・エリオツト・ビルダ
アンドリユ−・ジユリアン・スチユア−ト・ジヨ−ンズ
ノ−ム・スイン・シヤング・リン
ジヨン・ロバ−ト・オ−ゲズ
ケニス・チヤ−ルズ・オルソン
ロング−チヤング・パイ
ステイ−ブン・ヤコブ・シア
ロナルド・バ−ネル・ウエツゼル
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Genentech Inc
Original Assignee
Genentech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=27575443&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=JP2575604(B2) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Genentech Inc filed Critical Genentech Inc
Publication of JPS59161321A publication Critical patent/JPS59161321A/ja
Application granted granted Critical
Publication of JP2575604B2 publication Critical patent/JP2575604B2/ja
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6478—Aspartic endopeptidases (3.4.23)
    • C12N9/6481—Pepsins (3.4.23.1; 3.4.23.2; 3.4.23.3)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
    • C07K1/1133—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by redox-reactions involving cystein/cystin side chains
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6462—Plasminogen activators u-Plasminogen activator (3.4.21.73), i.e. urokinase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21073—Serine endopeptidases (3.4.21) u-Plasminogen activator (3.4.21.73), i.e. urokinase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Enzymes And Modification Thereof (AREA)
JP58241820A 1982-12-22 1983-12-21 沈澱異種蛋白質の精製及び活性化法 Expired - Lifetime JP2575604B2 (ja)

Applications Claiming Priority (16)

Application Number Priority Date Filing Date Title
US45235582A 1982-12-22 1982-12-22
US45218782A 1982-12-22 1982-12-22
US45225382A 1982-12-22 1982-12-22
US45235682A 1982-12-22 1982-12-22
US45225282A 1982-12-22 1982-12-22
US45235782A 1982-12-22 1982-12-22
US45236382A 1982-12-22 1982-12-22
US45234482A 1982-12-22 1982-12-22
US452252 1982-12-22
US452357 1982-12-22
US452253 1982-12-22
US452344 1982-12-22
US452363 1982-12-22
US452356 1982-12-22
US452187 1982-12-22
US452355 2003-06-02

Publications (2)

Publication Number Publication Date
JPS59161321A JPS59161321A (ja) 1984-09-12
JP2575604B2 true JP2575604B2 (ja) 1997-01-29

Family

ID=27575443

Family Applications (1)

Application Number Title Priority Date Filing Date
JP58241820A Expired - Lifetime JP2575604B2 (ja) 1982-12-22 1983-12-21 沈澱異種蛋白質の精製及び活性化法

Country Status (12)

Country Link
EP (1) EP0114506B1 (show.php)
JP (1) JP2575604B2 (show.php)
AU (1) AU568109B2 (show.php)
BR (1) BR8307086A (show.php)
CA (1) CA1219234A (show.php)
DE (1) DE3380850D1 (show.php)
GR (1) GR79124B (show.php)
HU (1) HU197357B (show.php)
IL (1) IL70474A (show.php)
MX (1) MX166885B (show.php)
NZ (1) NZ206615A (show.php)
PT (1) PT77866B (show.php)

Families Citing this family (87)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4342832A (en) * 1979-07-05 1982-08-03 Genentech, Inc. Method of constructing a replicable cloning vehicle having quasi-synthetic genes
US4476049A (en) * 1983-09-20 1984-10-09 Hoffmann-La Roche Inc. Method for the extraction of immune interferon
JPH064680B2 (ja) * 1985-01-09 1994-01-19 武田薬品工業株式会社 高濃度ヒトγ型インターフェロンフラグメント水溶液の製造法
JPS60155137A (ja) * 1984-01-23 1985-08-15 Takeda Chem Ind Ltd 高濃度ヒトγ型インタ−フエロン水溶液の製造法
US4530787A (en) * 1984-03-28 1985-07-23 Cetus Corporation Controlled oxidation of microbially produced cysteine-containing proteins
JPS60258129A (ja) * 1984-06-05 1985-12-20 Kyowa Hakko Kogyo Co Ltd インタ−フエロンの活性化法
DE3583361D1 (de) * 1984-06-14 1991-08-08 Ucp Gen Pharma Ag Verfahren zur herstellung von thrombin-inhibitoren.
CA1267615A (en) * 1984-08-27 1990-04-10 Dan Hadary Method for recovering purified growth hormones from genetically engineered microorganisms
US6291662B1 (en) 1984-12-05 2001-09-18 Amgen Inc. Recombinant methods for production of serine protease inhibitors and DNA sequences
US5871956A (en) * 1984-12-06 1999-02-16 Amgen Inc. Recombinant methods for production of serine inhibitors and DNA sequences useful for same
US5900400A (en) * 1984-12-06 1999-05-04 Amgen Inc. Serine protease inhibitor analogs
US6132990A (en) * 1984-12-06 2000-10-17 Amgen Boulder Inc. Recombinant methods for production of serine protease inhibitors and DNA sequences useful for same
HU204563B (en) * 1984-12-06 1992-01-28 Synergen Biolog Inc Process for producing recombinant serine-protease inhibitors and dns sequencyes for them
WO1986004608A1 (en) * 1985-02-05 1986-08-14 Synergen Biologicals, Inc. Metalloproteinase inhibitor sequence recombinant vector system for using same and recombinant-dna method for the manufacture of same
US4861868A (en) * 1985-02-22 1989-08-29 Monsanto Company Production of proteins in procaryotes
US4652630A (en) * 1985-02-22 1987-03-24 Monsanto Company Method of somatotropin naturation
US4731440A (en) * 1985-02-22 1988-03-15 Monsanto Company Method of somatotropin solubilization using dimethylsulfone and naturation
ES8800957A1 (es) * 1985-02-22 1987-12-01 Monsanto Co Un metodo para la solubilizacion y renaturalizacion de proteina somatotropina
US6084073A (en) * 1985-03-25 2000-07-04 Chiron Corporation Recombinant ricin toxin
FR2579223B1 (fr) * 1985-03-25 1988-12-09 Genetica Procede de preparation microbiologique de la serum-albumine humaine
DE3511011A1 (de) * 1985-03-27 1986-10-02 Hoechst Ag, 6230 Frankfurt Verfahren zur isolierung und reinigung von (alpha)-interferonen
GB8508340D0 (en) * 1985-03-29 1985-05-09 Creighton T E Production of protein
DE3683186D1 (de) * 1985-04-25 1992-02-13 Hoffmann La Roche Rekombinantes humaninterleukin-1.
JPH0640832B2 (ja) * 1985-05-10 1994-06-01 味の素株式会社 インタ−ロイキン２ポリペプチドの回収方法
DE3580590D1 (de) * 1985-05-29 1990-12-20 Green Cross Corp Herstellungsverfahren fuer heterogene proteine.
DE3685424D1 (de) 1985-06-26 1992-06-25 Upjohn Co Solubilisation und oxidation von somatotropin aus transformierten mikroorganismen.
US5248769A (en) * 1985-06-26 1993-09-28 Cetus Oncology Corporation Process for recovering refractile bodies containing heterologous proteins from microbial hosts
US4748234A (en) * 1985-06-26 1988-05-31 Cetus Corporation Process for recovering refractile bodies containing heterologous proteins from microbial hosts
US4766224A (en) * 1985-08-19 1988-08-23 International Minerals & Chemical Corp. Purification and activation of proteins from insoluble inclusion bodies
US4656255A (en) * 1985-09-09 1987-04-07 International Minerals & Chemical Corp. Protein recovery
DE3537708A1 (de) 1985-10-23 1987-04-23 Boehringer Mannheim Gmbh Verfahren zur aktivierung von t-pa nach expression in prokaryonten
US5453363A (en) * 1985-10-23 1995-09-26 Boehringer Mannheim Gmbh Process for the activation of t-PA or Ing after genetic expression in prokaryotes
US4766205A (en) * 1985-11-13 1988-08-23 Beatrice Companies, Inc. Method for isolation of recombinant polypeptides in biologically active forms
US4734362A (en) * 1986-02-03 1988-03-29 Cambridge Bioscience Corporation Process for purifying recombinant proteins, and products thereof
US5831022A (en) * 1986-02-18 1998-11-03 Hoffmann-La Roche Inc. Purification of recombinant human IL-1α
FR2594846B1 (fr) * 1986-02-21 1989-10-20 Genetica Procede de preparation de la serum albumine humaine mature
US4689401A (en) * 1986-03-06 1987-08-25 Cetus Corporation Method of recovering microbially produced recombinant ricin toxin a chain
DE3611817A1 (de) * 1986-04-08 1987-10-15 Boehringer Mannheim Gmbh Verfahren zur renaturierung von proteinen
US4705848A (en) * 1986-06-02 1987-11-10 International Minerals & Chemical Corp. Isolation of bioactive, monomeric growth hormone
DE3618817A1 (de) * 1986-06-04 1987-12-10 Behringwerke Ag Verfahren zur gewinnung aktiver proteine aus einer biologisch inaktiven form
US4801686A (en) * 1986-09-04 1989-01-31 Immunex Corporation Purification of recombinant interleukin-1
US5179199A (en) * 1986-10-20 1993-01-12 Genzyme Corporation Protein purification
AU612133B2 (en) * 1987-02-20 1991-07-04 Natinco Nv Production of proteins in active forms
US5026828A (en) * 1987-02-27 1991-06-25 Merck & Co., Inc. Method of purifying recombinant pres-1/S-2/S/S hepatitis B antigen from yeast
US4929700A (en) * 1987-04-16 1990-05-29 Cetus Corporation Production of purified, biologically active, bacterially produced recombinant human CSF-1
IL86090A (en) * 1987-04-16 1993-03-15 Cetus Oncology Corp Production of purified, biologically active, bacterially produced recombinant human csf-1
CA1339757C (en) * 1987-04-16 1998-03-17 Robert F. Halenbeck Production of purified biologically active, bacterially produced recombinant human csf-1
AU621051B2 (en) * 1987-04-28 1992-03-05 Amgen, Inc. Method for purifying granulocyte-macrophage colony stimulating factor
US5162507A (en) * 1987-05-11 1992-11-10 Cetus Corporation Process for recovering purified, oxidized, renatured recombinant interleukin-2 from microorganisms
US4931543A (en) * 1987-05-11 1990-06-05 Cetus Corporation Process for recovering microbially produced interleukin-2
ATE156491T1 (de) * 1987-05-11 1997-08-15 Chiron Corp Prozess zur gewinnung von gereinigtem, oxidiertem, renaturiertem, rekombinantem interleukin-2 aus mikroorganismen
JP2669859B2 (ja) * 1987-08-04 1997-10-29 協和醗酵工業株式会社 タンパク質の再活性化法
US4985544A (en) * 1987-08-04 1991-01-15 Kyowa Hakko Kogyo Co., Ltd. Process for renaturing fish growth hormone
US5011915A (en) * 1987-10-26 1991-04-30 Merck & Co., Inc. Process for purifying recombinant hepatitis antigens
EP0325691B1 (de) * 1988-01-25 1993-04-07 Consortium für elektrochemische Industrie GmbH Verfahren zur Solubilisierung von unlöslichem Protein
JPH01257491A (ja) * 1988-04-08 1989-10-13 Tosoh Corp 不溶性融合異種蛋白質の処理方法
US4999422A (en) * 1988-04-15 1991-03-12 Biogen, N.V. Continuous method of refolding proteins
EP0377023B1 (en) * 1988-05-31 1995-02-15 Cetus Oncology Corporation Process for recovering refractile bodies containing heterologous proteins from microbial hosts
US5089473A (en) 1988-08-29 1992-02-18 Monsanto Company Somatotropin variants and their use
CA1340586C (en) * 1988-09-23 1999-06-08 Cetus Corporation Process for recovering microbially produced interferon-beta
DE3832898A1 (de) * 1988-09-28 1990-04-12 Boehringer Mannheim Gmbh Praeparat von in prokaryonten exprimiertem plasminogenaktivator
DE3835350A1 (de) * 1988-10-17 1990-04-19 Boehringer Mannheim Gmbh Aktivierung von gentechnologisch hergestellten, in prokaryonten exprimierten antikoerpern
US5179196A (en) * 1989-05-04 1993-01-12 Sri International Purification of proteins employing ctap-iii fusions
GB8927546D0 (en) * 1989-12-06 1990-02-07 Ciba Geigy Process for the production of biologically active tgf-beta
DE4039415A1 (de) 1990-02-03 1991-08-08 Boehringer Mannheim Gmbh Verfahren zur herstellung rekombinanter proteine ohne n-terminalen methioninrest
US5023323A (en) * 1990-09-12 1991-06-11 Monsanto Company Method of somatotropin naturation
JPH04113933U (ja) * 1991-03-22 1992-10-06 富泰 本多 化粧板
JPH084290Y2 (ja) * 1991-03-22 1996-02-07 富泰 本多 化粧板
US6869925B1 (en) * 1992-09-09 2005-03-22 Amgen Inc. Inhibition of retrovirus infection
US5399677A (en) * 1993-12-07 1995-03-21 Genetics Institute, Inc. Mutants of bone morphogenetic proteins
US6017880A (en) * 1994-03-09 2000-01-25 Amgen Inc. Inhibition of retrovirus infection
US5437986A (en) * 1994-06-22 1995-08-01 Schering Corporation Extraction of heterologous insoluble proteins from bacteria
CA2194177A1 (en) * 1994-07-25 1996-02-08 Jui Yoa Chang Process for folding of proteins like recombinant hirudin or epidermal growth factor
TW517059B (en) 1994-07-25 2003-01-11 Ciba Geigy Ag New process for the production of biologically active protein
US6037145A (en) * 1994-09-07 2000-03-14 Suntory Limited Process for production of protein
GB9425138D0 (en) 1994-12-12 1995-02-08 Dynal As Isolation of nucleic acid
AU714318B2 (en) * 1996-06-11 2000-01-06 Roche Diagnostics Gmbh Process for the activation of denatured protein
US5914390A (en) * 1997-05-12 1999-06-22 Celtrix Pharmaceuticals, Inc. Methods for increasing yields of recombinant proteins
WO1999010483A2 (de) * 1997-08-22 1999-03-04 Roche Diagnostics Gmbh Autokatalytisch aktivierbare vorstufen von proteasen und deren verwendung
JP3734634B2 (ja) 1999-03-03 2006-01-11 ヒゲタ醤油株式会社 タンパク質の活性化方法及び該装置
IN187900B (show.php) * 2000-02-01 2002-07-20 Torrent Pharmaceuticals Ltd
KR100409092B1 (ko) * 2000-12-22 2003-12-11 주)녹십자 단백질의 제조 방법
CA2540446A1 (en) 2003-09-30 2005-04-07 Daiichi Asubio Pharma Co., Ltd. Polypeptide cleavage method using ompt protease variant
US20070021591A1 (en) * 2005-07-25 2007-01-25 Trubion Pharmaceuticals, Inc. Compositions and methods for protein deaggregation
BR112012022518A2 (pt) 2010-03-17 2020-10-13 Biogenerix Gmbh método para obtenção de g- csf recombinante humano biologicamente ativo
JP5805943B2 (ja) * 2010-12-08 2015-11-10 株式会社北里大塚バイオメディカルアッセイ研究所 百日咳菌のＦｉｍ３の製造方法、及び、百日咳の検出方法
USD1086051S1 (en) * 2022-09-30 2025-07-29 Maxcyte, Inc. Electroporation gasket

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4350764A (en) * 1980-03-10 1982-09-21 The Regents Of The University Of California Microbiological synthesis of beta endorphin
DK170476B1 (da) * 1980-04-03 1995-09-11 Biogen Inc Rekombinant DNA-molekyle, uni-cellulær vært transformeret med mindst ét rekombinant DNA-molekyle, fremgangsmåde til transformering af en uni-cellulær vært, samt fremgangsmåder til fremstilling af et polpeptid, der udviser den immunologiske eller biologiske aktivitet af human beta-interferon
NZ198445A (en) * 1980-09-25 1984-05-31 Genentech Inc Production of human fibroblast interferon by recombinant dna technology
AU561343B2 (en) * 1981-10-19 1987-05-07 Genentech Inc. Human immune interferon by recombinant dna
JPS58157800A (ja) * 1982-03-16 1983-09-19 Sankyo Co Ltd プラスミドｐｓａｎ１８１

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
Ｂｉｏｃｈｅｍｉｓｔｒｙ，９［２５］Ｐ．５０１５−５０２２３
Ｊ．Ｂｉｏｌ，Ｃｈｅｍ，２５０［２１］Ｐ．８４７７−８４８２
Ｊ．Ｂｉｏｌ，Ｃｈｅｍ，２５３［１０］（１９７８）Ｐ．３４５３−３４５８

Also Published As

Publication number Publication date
IL70474A (en) 1989-09-28
HU197357B (en) 1989-03-28
PT77866B (en) 1986-04-09
GR79124B (show.php) 1984-10-02
EP0114506A1 (en) 1984-08-01
EP0114506B1 (en) 1989-11-15
CA1219234A (en) 1987-03-17
PT77866A (en) 1984-01-01
MX166885B (es) 1993-02-10
AU568109B2 (en) 1987-12-17
JPS59161321A (ja) 1984-09-12
NZ206615A (en) 1988-03-30
AU2259483A (en) 1984-06-28
DE3380850D1 (en) 1989-12-21
IL70474A0 (en) 1984-03-30
BR8307086A (pt) 1985-02-12

Similar Documents

Publication Publication Date Title
EP0114506B1 (en) Methods of purification and reactivation of precipitated heterologous proteins
US4512922A (en) Purification and activity assurance of precipitated heterologous proteins
US4620948A (en) Purification and activity assurance of precipitated heterologous proteins
US4511502A (en) Purification and activity assurance of precipitated heterologous proteins
US4518526A (en) Purification and activity assurance of precipitated heterologous proteins
US4511503A (en) Purification and activity assurance of precipitated heterologous proteins
US4599197A (en) Purification and activity assurance of precipitated heterologous proteins
US4766224A (en) Purification and activation of proteins from insoluble inclusion bodies
EP0279619B1 (en) Production of proteins in active forms
EP0215625B1 (en) Protein recovery
JPH06102034B2 (ja) タンパク質の生産方法
AU593548B2 (en) A process for the production of a protein
NO863424L (no) Fremgangsmaate for rensing av et interferon.
IE66334B1 (en) Production of proteins in active forms
EP0173215B1 (en) Method for recovering purified growth hormones from genetically engineered microorganisms
JPH01257491A (ja) 不溶性融合異種蛋白質の処理方法
US5340926A (en) Process for the recovery of recombinantly produced protein from insoluble aggregate
HUP0002573A2 (hu) Eljárás csont eredetű faktor tisztított dimerjének előállítására
JP2517100B2 (ja) ヒト顆粒球コロニ―刺激因子活性を有する蛋白質の精製法
JP3930051B2 (ja) 正しく折畳まれた生物学的に活性の組換えタンパク質の製造方法
IE56376B1 (en) Methods of purification and reactivation of precipitated heterologous proteins
RU2123010C1 (ru) Способ получения рекомбинантного интерферона-альфа-2 из нерастворимых тел включения
Lutsenko et al. Development of the method of isolation and purification of the recombinant human interleukin-7
JPH02227076A (ja) 遺伝子組換え菌で製造された不溶性蛋白質の活性化法
JPH04320693A (ja) タンパクの精製方法