JP2019518759A5 - - Google Patents

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Publication number
JP2019518759A5
JP2019518759A5 JP2018565677A JP2018565677A JP2019518759A5 JP 2019518759 A5 JP2019518759 A5 JP 2019518759A5 JP 2018565677 A JP2018565677 A JP 2018565677A JP 2018565677 A JP2018565677 A JP 2018565677A JP 2019518759 A5 JP2019518759 A5 JP 2019518759A5
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JP
Japan
Prior art keywords
target oligonucleotide
product
impurity
mixture
hydrophobic adsorbent
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JP2018565677A
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English (en)
Japanese (ja)
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JP2019518759A (ja
JP7075359B2 (ja
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Priority claimed from PCT/US2017/037126 external-priority patent/WO2017218454A1/en
Publication of JP2019518759A publication Critical patent/JP2019518759A/ja
Publication of JP2019518759A5 publication Critical patent/JP2019518759A5/ja
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Publication of JP7075359B2 publication Critical patent/JP7075359B2/ja
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JP2018565677A 2016-06-14 2017-06-13 オリゴヌクレオチドの精製のための疎水性相互作用クロマトグラフィー Active JP7075359B2 (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201662349970P 2016-06-14 2016-06-14
US62/349,970 2016-06-14
US201762492402P 2017-05-01 2017-05-01
US62/492,402 2017-05-01
PCT/US2017/037126 WO2017218454A1 (en) 2016-06-14 2017-06-13 Hydrophobic interaction chromatography for purification of oligonucleotides

Publications (3)

Publication Number Publication Date
JP2019518759A JP2019518759A (ja) 2019-07-04
JP2019518759A5 true JP2019518759A5 (enExample) 2022-04-07
JP7075359B2 JP7075359B2 (ja) 2022-05-25

Family

ID=59216016

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2018565677A Active JP7075359B2 (ja) 2016-06-14 2017-06-13 オリゴヌクレオチドの精製のための疎水性相互作用クロマトグラフィー

Country Status (11)

Country Link
US (3) US10947262B2 (enExample)
EP (1) EP3468981B1 (enExample)
JP (1) JP7075359B2 (enExample)
KR (1) KR102461234B1 (enExample)
CN (1) CN109641928B (enExample)
AU (1) AU2017284202B2 (enExample)
BR (1) BR112018075667B1 (enExample)
ES (1) ES2988362T3 (enExample)
IL (1) IL263639B (enExample)
MX (1) MX2018015654A (enExample)
WO (1) WO2017218454A1 (enExample)

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Publication number Priority date Publication date Assignee Title
WO2016040748A1 (en) 2014-09-12 2016-03-17 Ionis Pharmaceuticals, Inc. Compositions and methods for detection of smn protein in a subject and treatment of a subject
AR102198A1 (es) 2014-10-09 2017-02-08 Regeneron Pharma Proceso para reducir partículas subvisibles en una formulación farmacéutica
AU2017312785B2 (en) 2016-08-16 2024-10-10 Regeneron Pharmaceuticals, Inc. Methods for quantitating individual antibodies from a mixture
KR102759512B1 (ko) 2016-10-25 2025-02-03 리제너론 파아마슈티컬스, 인크. 크로마토그래피 데이터 분석을 위한 방법 및 시스템
WO2019060062A1 (en) 2017-09-19 2019-03-28 Regeneron Pharmaceuticals Inc. METHODS OF REDUCING PARTICLE FORMATION AND COMPOSITIONS FORMED THEREFROM
TWI919355B (zh) 2018-07-02 2026-03-21 美商里珍納龍藥品有限公司 自混合物製備多肽之系統及方法
WO2020072062A1 (en) * 2018-10-04 2020-04-09 Gsw Creative Corporation Portable charging case
EA202192422A1 (ru) 2019-03-05 2021-12-09 Ридженерон Фармасьютикалз, Инк. Человеческий сывороточный альбумин в составах
CN110066789B (zh) * 2019-05-17 2021-04-20 通用生物系统（安徽）有限公司 一种长链dna引物的hplc纯化改进的方法
CN114025805B (zh) * 2019-06-07 2025-01-24 达因疗法公司 制备蛋白质-寡核苷酸复合物的方法
AU2021225957A1 (en) 2020-02-28 2022-09-08 Ionis Pharmaceuticals, Inc. Compounds and methods for modulating SMN2
WO2022187312A1 (en) * 2021-03-02 2022-09-09 Ionis Pharmaceuticals, Inc. Purification methods for oligomeric compounds
JP7897322B2 (ja) * 2022-09-07 2026-07-29 株式会社日本触媒 核酸の製造方法、不純物の除去方法及び不純物が少ない核酸
CN117050120A (zh) * 2022-12-09 2023-11-14 苏州赛分科技股份有限公司 一种纯化小干扰rna的柱层析法
WO2024175051A1 (zh) * 2023-02-23 2024-08-29 奥安医药（苏州）有限公司 一种疏水层析制备寡核苷酸的方法
WO2025149349A1 (en) 2024-01-09 2025-07-17 Cytiva Bioprocess R&D Ab Method for separating nucleic acid molecules
WO2025234897A1 (ru) * 2024-05-07 2025-11-13 Акционерное общество "ГЕНЕРИУМ" Способ получения тиофосфатных олигонуклеотидов для терапевтического применения

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JPS60104097A (ja) * 1983-11-08 1985-06-08 Sagami Chem Res Center Ｄνａの精製方法
DE3433649A1 (de) * 1984-09-13 1986-03-20 Gesellschaft für Biotechnologische Forschung mbH (GBF), 3300 Braunschweig Verfahren zur aufreinigung synthetischer oligonucleotide
US6087491A (en) * 1993-01-08 2000-07-11 Hybridon, Inc. Extremely high purity oligonucleotides and methods of synthesizing them using dimer blocks
CN1155887A (zh) * 1994-07-05 1997-07-30 海布里顿公司 用deae-5pw阴离子-离子交换色谱和疏水作用色谱纯化硫代磷酸寡脱氧核苷酸
WO1996001268A1 (en) * 1994-07-05 1996-01-18 Hybridon, Inc. Purification of oligodeoxynucleotide phosphorothioates using deae 5pw anion ion-exchange chromatography and hydrophobic interaction chromatography
JP2001520660A (ja) * 1997-04-21 2001-10-30 プロリゴ・エルエルシー オリゴヌクレオチドの溶液相合成方法
JP2000035423A (ja) 1998-05-11 2000-02-02 Tosoh Corp 液体クロマトグラフィ―による核酸の分離法
CN1264854C (zh) * 1999-05-28 2006-07-19 坎布雷科斯生物科学巴尔的摩公司 Dna纯化方法和纯化的dna
PT102491B (pt) * 2000-07-10 2003-02-28 Inst Superior Tecnico Processo para producao e purificacao de dna plasmidico
US7345163B2 (en) 2002-08-28 2008-03-18 Quiatech Ab Process for separating and deprotecting oligonucleotides
CA2504694C (en) 2002-11-05 2013-10-01 Isis Pharmaceuticals, Inc. Polycyclic sugar surrogate-containing oligomeric compounds and compositions for use in gene modulation
US20050019951A1 (en) * 2003-07-14 2005-01-27 Gjerde Douglas T. Method and device for extracting an analyte
DK1661905T3 (da) 2003-08-28 2012-07-23 Takeshi Imanishi Hidtil ukendte syntetiske nukleinsyrer af N-O-krydsbindingstype
MXPA06011568A (es) * 2004-04-19 2006-12-20 Centelion Metodo para purificar adn plasmidico.
PT2548560E (pt) 2005-06-23 2015-09-22 Isis Pharmaceuticals Inc Composições e métodos de modulação da excisão-união de smn2
DE602007009487D1 (de) 2006-01-27 2010-11-11 Isis Pharmaceutical Inc 6-modifizierte bicyclische nukleinsäureanaloga
SG2014015010A (en) * 2008-04-30 2014-05-29 Gradalis Inc Highly pure plasmid dna preparations and processes for preparing the same
KR20220084437A (ko) 2009-06-17 2022-06-21 바이오젠 엠에이 인코포레이티드 대상에게서 ｓｍｎ２ 스플라이싱을 조정하기 위한 조성물 및 방법
HUE050704T2 (hu) 2014-04-01 2020-12-28 Biogen Ma Inc Összetételek a SOD-1 expressziójának modulálására

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