PT102491B - Processo para producao e purificacao de dna plasmidico - Google Patents

Processo para producao e purificacao de dna plasmidico

Info

Publication number
PT102491B
PT102491B PT102491A PT10249100A PT102491B PT 102491 B PT102491 B PT 102491B PT 102491 A PT102491 A PT 102491A PT 10249100 A PT10249100 A PT 10249100A PT 102491 B PT102491 B PT 102491B
Authority
PT
Portugal
Prior art keywords
purification
production
plasmidic dna
plasmidic
dna
Prior art date
Application number
PT102491A
Other languages
English (en)
Other versions
PT102491A (pt
Inventor
Duarte Miguel De Fran Prazeres
Maria Margarida Fonseca Diogo
Joao Antonio De Sampai Queiroz
Original Assignee
Inst Superior Tecnico
Univ Da Beira Interior
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Inst Superior Tecnico, Univ Da Beira Interior filed Critical Inst Superior Tecnico
Priority to PT102491A priority Critical patent/PT102491B/pt
Priority to PCT/PT2001/000012 priority patent/WO2002004027A1/en
Priority to US10/332,633 priority patent/US7169917B2/en
Publication of PT102491A publication Critical patent/PT102491A/pt
Publication of PT102491B publication Critical patent/PT102491B/pt

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Biophysics (AREA)
  • Public Health (AREA)
  • Plant Pathology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Epidemiology (AREA)
  • Medicinal Chemistry (AREA)
  • Veterinary Medicine (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
PT102491A 2000-07-10 2000-07-10 Processo para producao e purificacao de dna plasmidico PT102491B (pt)

Priority Applications (3)

Application Number Priority Date Filing Date Title
PT102491A PT102491B (pt) 2000-07-10 2000-07-10 Processo para producao e purificacao de dna plasmidico
PCT/PT2001/000012 WO2002004027A1 (en) 2000-07-10 2001-07-06 Purification of plasmid dna by hydrophobic interaction chromatography
US10/332,633 US7169917B2 (en) 2000-07-10 2001-07-06 Purification of plasmid DNA by hydrophobic interaction chromatography

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PT102491A PT102491B (pt) 2000-07-10 2000-07-10 Processo para producao e purificacao de dna plasmidico

Publications (2)

Publication Number Publication Date
PT102491A PT102491A (pt) 2002-01-30
PT102491B true PT102491B (pt) 2003-02-28

Family

ID=20085969

Family Applications (1)

Application Number Title Priority Date Filing Date
PT102491A PT102491B (pt) 2000-07-10 2000-07-10 Processo para producao e purificacao de dna plasmidico

Country Status (3)

Country Link
US (1) US7169917B2 (pt)
PT (1) PT102491B (pt)
WO (1) WO2002004027A1 (pt)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1462519A1 (en) 2003-03-24 2004-09-29 Boehringer Ingelheim Austria GmbH Method and devices for producing biomolecules
US8501402B2 (en) 2003-03-24 2013-08-06 Boehringer Ingelheim Rcv Gmbh & Co Kg Methods and devices for producing biomolecules
US20090047734A1 (en) * 2006-03-31 2009-02-19 Ge Healthcare Bio-Sciences Ab Method of separation of deoxyribonucleic acids
CN101702938A (zh) * 2007-05-23 2010-05-05 Vgx药品公司 包含高浓度的生物活性分子的组合物及其制备工艺
EP2088196A1 (en) 2008-02-08 2009-08-12 Boehringer Ingelheim RCV GmbH & Co KG Methods and devices for producing biomolecules
AU2009242587B2 (en) 2008-04-30 2015-05-28 Strike Bio, Inc. Highly pure plasmid DNA preparations and processes for preparing the same
BR112018075667B1 (pt) * 2016-06-14 2024-02-20 Biogen Ma, Inc Métodos para separar um oligonucleotídeo alvo de uma mistura contendo o oligonucleotídeo alvo e uma impureza relacionada com o produto
CN109294968B (zh) * 2017-07-25 2022-06-14 格兰柏生化科技有限公司 一种动物核酸疫苗的大规模生产技术
US20230167457A1 (en) 2019-12-20 2023-06-01 Engenes Biotech Gmbh Continuous bioproduction by decoupling growth and production
WO2022073995A1 (en) * 2020-10-05 2022-04-14 Bia Separations D.O.O. Improved removal of rna and contaminants from dna plasmid preparations by hydrophobic interaction chromatography
CN114907430B (zh) * 2022-06-21 2023-12-01 中国科学院过程工程研究所 一种mRNA的分离纯化方法
EP4299753A1 (en) 2022-06-29 2024-01-03 enGenes Biotech GmbH Method for producing extrachromosomal nucleic acids
US20250327106A1 (en) 2022-06-29 2025-10-23 Takeda Pharmaceutical Company Limited Method for producing extrachromosomal nucleic acids
CN115125240B (zh) * 2022-07-29 2025-08-01 苏州纳微科技股份有限公司 一种质粒dna工业化分离纯化方法及其应用
WO2025166642A1 (zh) * 2024-02-07 2025-08-14 首都医科大学 高生物活性质粒dna及其制备和存储、疫苗、试剂盒及其应用

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5561064A (en) * 1994-02-01 1996-10-01 Vical Incorporated Production of pharmaceutical-grade plasmid DNA
US5981735A (en) * 1996-02-12 1999-11-09 Cobra Therapeutics Limited Method of plasmid DNA production and purification
US5707812A (en) 1996-08-06 1998-01-13 Vical Incorporated Purification of plasmid DNA during column chromatography
GB9709728D0 (en) * 1997-05-13 1997-07-02 Dynal As Single step method

Also Published As

Publication number Publication date
WO2002004027A1 (en) 2002-01-17
US20040038393A1 (en) 2004-02-26
US7169917B2 (en) 2007-01-30
PT102491A (pt) 2002-01-30

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Legal Events

Date Code Title Description
BB1A Laying open of patent application

Effective date: 20001128

FG3A Patent granted, date of granting

Effective date: 20021227