MX2018015654A - Cromatografía de interacción hidrofóbica para la purificación de oligonucleótidos. - Google Patents

Cromatografía de interacción hidrofóbica para la purificación de oligonucleótidos.

Info

Publication number
MX2018015654A
MX2018015654A MX2018015654A MX2018015654A MX2018015654A MX 2018015654 A MX2018015654 A MX 2018015654A MX 2018015654 A MX2018015654 A MX 2018015654A MX 2018015654 A MX2018015654 A MX 2018015654A MX 2018015654 A MX2018015654 A MX 2018015654A
Authority
MX
Mexico
Prior art keywords
oligonucleotides
hydrophobic interaction
purification
interaction chromatography
chromatography
Prior art date
Application number
MX2018015654A
Other languages
English (en)
Inventor
S Gronke Robert
S Joshi Ratnesh
Original Assignee
Biogen Ma Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biogen Ma Inc filed Critical Biogen Ma Inc
Publication of MX2018015654A publication Critical patent/MX2018015654A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00—Processes for the preparation of sugar derivatives
    • C07H1/06—Separation; Purification
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/30—Partition chromatography
    • B01D15/305—Hydrophilic interaction chromatography [HILIC]
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/32—Bonded phase chromatography
    • B01D15/325—Reversed phase
    • B01D15/327—Reversed phase with hydrophobic interaction
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/42—Selective adsorption, e.g. chromatography characterised by the development mode, e.g. by displacement or by elution
    • B01D15/424—Elution mode
    • B01D15/426—Specific type of solvent
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00—Processes for the preparation of sugar derivatives
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/02—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with ribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • Saccharide Compounds (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

La presente invención se dirige a un método de purificación de oligonucleótidos mediante cromatografía de interacción hidrofóbica.
MX2018015654A 2016-06-14 2017-06-13 Cromatografía de interacción hidrofóbica para la purificación de oligonucleótidos. MX2018015654A (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201662349970P 2016-06-14 2016-06-14
US201762492402P 2017-05-01 2017-05-01
PCT/US2017/037126 WO2017218454A1 (en) 2016-06-14 2017-06-13 Hydrophobic interaction chromatography for purification of oligonucleotides

Publications (1)

Publication Number Publication Date
MX2018015654A true MX2018015654A (es) 2019-06-10

Family

ID=59216016

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2018015654A MX2018015654A (es) 2016-06-14 2017-06-13 Cromatografía de interacción hidrofóbica para la purificación de oligonucleótidos.

Country Status (11)

Country Link
US (2) US10947262B2 (es)
EP (1) EP3468981B1 (es)
JP (1) JP7075359B2 (es)
KR (1) KR102461234B1 (es)
CN (1) CN109641928B (es)
AU (1) AU2017284202B2 (es)
BR (1) BR112018075667B1 (es)
CA (1) CA3027288A1 (es)
IL (1) IL263639B (es)
MX (1) MX2018015654A (es)
WO (1) WO2017218454A1 (es)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2016040748A1 (en) 2014-09-12 2016-03-17 Ionis Pharmaceuticals, Inc. Compositions and methods for detection of smn protein in a subject and treatment of a subject
TW201628649A (zh) 2014-10-09 2016-08-16 再生元醫藥公司 減少醫藥調配物中微可見顆粒之方法
IL264631B2 (en) 2016-08-16 2024-05-01 Regeneron Pharma A method for quantifying individual antibodies from a mixture
SG11201902667UA (en) 2016-10-25 2019-05-30 Regeneron Pharma Methods and systems for chromatography data analysis
SG11202001564QA (en) 2017-09-19 2020-04-29 Regeneron Pharma Methods of reducing particle formation and compositions formed thereby
US11884698B2 (en) 2018-07-02 2024-01-30 Regeneron Pharmaceuticals, Inc. Systems and methods for preparing a polypeptide from a mixture
WO2020072062A1 (en) * 2018-10-04 2020-04-09 Gsw Creative Corporation Portable charging case
CN110066789B (zh) * 2019-05-17 2021-04-20 通用生物系统（安徽）有限公司 一种长链dna引物的hplc纯化改进的方法
US20220306685A1 (en) * 2019-06-07 2022-09-29 Dyne Therapeutics, Inc. Methods of preparing protein-oligonucleotide complexes
MX2022010602A (es) 2020-02-28 2022-09-09 Ionis Pharmaceuticals Inc Compuestos y metodos para modular smn2.
US20240092822A1 (en) * 2021-03-02 2024-03-21 Ionis Pharmaceuticals, Inc. Purification methods for oligomeric compounds
WO2024053578A1 (ja) * 2022-09-07 2024-03-14 株式会社日本触媒 核酸の製造方法、不純物の除去方法及び不純物が少ない核酸
WO2024175051A1 (zh) * 2023-02-23 2024-08-29 奥安医药（苏州）有限公司 一种疏水层析制备寡核苷酸的方法

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JPS60104097A (ja) * 1983-11-08 1985-06-08 Sagami Chem Res Center Ｄνａの精製方法
DE3433649A1 (de) * 1984-09-13 1986-03-20 Gesellschaft für Biotechnologische Forschung mbH (GBF), 3300 Braunschweig Verfahren zur aufreinigung synthetischer oligonucleotide
US6087491A (en) * 1993-01-08 2000-07-11 Hybridon, Inc. Extremely high purity oligonucleotides and methods of synthesizing them using dimer blocks
CN1155887A (zh) * 1994-07-05 1997-07-30 海布里顿公司 用deae-5pw阴离子-离子交换色谱和疏水作用色谱纯化硫代磷酸寡脱氧核苷酸
JPH10505577A (ja) * 1994-07-05 1998-06-02 ハイブライドン インコーポレイテッド Ｄｅａｅ ５ｐｗ陰イオン・イオン交換クロマトグラフィー及び疎水性相互作用クロマトグラフィーを用いるオリゴデオキシヌクレオチドホスホロチオエートの精製
WO1998047910A1 (en) * 1997-04-21 1998-10-29 Proligo Llc Method for solution phase synthesis of oligonucleotides
JP2000035423A (ja) 1998-05-11 2000-02-02 Tosoh Corp 液体クロマトグラフィ―による核酸の分離法
IL146695A0 (en) * 1999-05-28 2002-07-25 Bio Science Contract Productio Methods of dna purification and purified dna
PT102491B (pt) * 2000-07-10 2003-02-28 Inst Superior Tecnico Processo para producao e purificacao de dna plasmidico
US7345163B2 (en) 2002-08-28 2008-03-18 Quiatech Ab Process for separating and deprotecting oligonucleotides
CA2504694C (en) 2002-11-05 2013-10-01 Isis Pharmaceuticals, Inc. Polycyclic sugar surrogate-containing oligomeric compounds and compositions for use in gene modulation
US20050019951A1 (en) * 2003-07-14 2005-01-27 Gjerde Douglas T. Method and device for extracting an analyte
JP4731324B2 (ja) 2003-08-28 2011-07-20 武 今西 Ｎ−ｏ結合性架橋構造型新規人工核酸
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HUE027486T2 (en) 2005-06-23 2016-09-28 Isis Pharmaceuticals Inc Preparations and methods for modifying SMN2-Splicing
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Also Published As

Publication number Publication date
JP2019518759A (ja) 2019-07-04
CN109641928A (zh) 2019-04-16
CN109641928B (zh) 2022-08-30
EP3468981A1 (en) 2019-04-17
IL263639A (en) 2019-01-31
US20190248823A1 (en) 2019-08-15
AU2017284202B2 (en) 2021-11-04
AU2017284202A1 (en) 2019-01-17
BR112018075667A2 (pt) 2019-04-09
EP3468981B1 (en) 2024-07-24
WO2017218454A1 (en) 2017-12-21
KR102461234B1 (ko) 2022-10-28
IL263639B (en) 2021-09-30
JP7075359B2 (ja) 2022-05-25
US20210269467A1 (en) 2021-09-02
CA3027288A1 (en) 2017-12-21
US10947262B2 (en) 2021-03-16
KR20190018467A (ko) 2019-02-22
BR112018075667B1 (pt) 2024-02-20

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