BR112018075667A2 - cromatografia de interação hidrofóbica para purificação de oligonucleotídeos - Google Patents

cromatografia de interação hidrofóbica para purificação de oligonucleotídeos

Info

Publication number
BR112018075667A2
BR112018075667A2 BR112018075667A BR112018075667A BR112018075667A2 BR 112018075667 A2 BR112018075667 A2 BR 112018075667A2 BR 112018075667 A BR112018075667 A BR 112018075667A BR 112018075667 A BR112018075667 A BR 112018075667A BR 112018075667 A2 BR112018075667 A2 BR 112018075667A2
Authority
BR
Brazil
Prior art keywords
hydrophobic interaction
interaction chromatography
oligonucleotide purification
oligonucleotide
purification
Prior art date
Application number
BR112018075667A
Other languages
English (en)
Other versions
BR112018075667B1 (pt
Inventor
S Joshi Ratnesh
S Gronke Robert
Original Assignee
Biogen Ma Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biogen Ma Inc filed Critical Biogen Ma Inc
Publication of BR112018075667A2 publication Critical patent/BR112018075667A2/pt
Publication of BR112018075667B1 publication Critical patent/BR112018075667B1/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00—Processes for the preparation of sugar derivatives
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00—Processes for the preparation of sugar derivatives
    • C07H1/06—Separation; Purification
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/30—Partition chromatography
    • B01D15/305—Hydrophilic interaction chromatography [HILIC]
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/32—Bonded phase chromatography
    • B01D15/325—Reversed phase
    • B01D15/327—Reversed phase with hydrophobic interaction
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/42—Selective adsorption, e.g. chromatography characterised by the development mode, e.g. by displacement or by elution
    • B01D15/424—Elution mode
    • B01D15/426—Specific type of solvent
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/02—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with ribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biochemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Saccharide Compounds (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

a presente invenção refere-se a um método de purificação de oligonucleotídeos utilizando cromatografia de interação hidrofóbica.
BR112018075667-9A 2016-06-14 2017-06-13 Métodos para separar um oligonucleotídeo alvo de uma mistura contendo o oligonucleotídeo alvo e uma impureza relacionada com o produto BR112018075667B1 (pt)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201662349970P 2016-06-14 2016-06-14
US62/349,970 2016-06-14
US201762492402P 2017-05-01 2017-05-01
US62/492,402 2017-05-01
PCT/US2017/037126 WO2017218454A1 (en) 2016-06-14 2017-06-13 Hydrophobic interaction chromatography for purification of oligonucleotides

Publications (2)

Publication Number Publication Date
BR112018075667A2 true BR112018075667A2 (pt) 2019-04-09
BR112018075667B1 BR112018075667B1 (pt) 2024-02-20

Family

ID=59216016

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112018075667-9A BR112018075667B1 (pt) 2016-06-14 2017-06-13 Métodos para separar um oligonucleotídeo alvo de uma mistura contendo o oligonucleotídeo alvo e uma impureza relacionada com o produto

Country Status (11)

Country Link
US (2) US10947262B2 (pt)
EP (1) EP3468981B1 (pt)
JP (1) JP7075359B2 (pt)
KR (1) KR102461234B1 (pt)
CN (1) CN109641928B (pt)
AU (1) AU2017284202B2 (pt)
BR (1) BR112018075667B1 (pt)
CA (1) CA3027288A1 (pt)
IL (1) IL263639B (pt)
MX (1) MX2018015654A (pt)
WO (1) WO2017218454A1 (pt)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10436802B2 (en) 2014-09-12 2019-10-08 Biogen Ma Inc. Methods for treating spinal muscular atrophy
AR102198A1 (es) 2014-10-09 2017-02-08 Regeneron Pharma Proceso para reducir partículas subvisibles en una formulación farmacéutica
BR112019000872A2 (pt) 2016-08-16 2019-04-30 Regeneron Pharmaceuticals, Inc. métodos para quantificar anticorpos individuais de uma mistura
IL312194A (en) 2016-10-25 2024-06-01 Regeneron Pharma Methods and systems for analysis of chromatography data
CA3073935A1 (en) 2017-09-19 2019-03-28 Regeneron Pharmaceuticals, Inc. Methods of reducing particle formation and compositions formed thereby
BR112020025624A2 (pt) 2018-07-02 2021-03-23 Regeneron Pharmaceuticals, Inc. uso de cromatografia de interação hidrofóbica múltipla para preparar um polipeptídeo a partir de uma mistura
WO2020072062A1 (en) * 2018-10-04 2020-04-09 Gsw Creative Corporation Portable charging case
CN110066789B (zh) * 2019-05-17 2021-04-20 通用生物系统（安徽）有限公司 一种长链dna引物的hplc纯化改进的方法
US20220306685A1 (en) * 2019-06-07 2022-09-29 Dyne Therapeutics, Inc. Methods of preparing protein-oligonucleotide complexes
CR20220485A (es) 2020-02-28 2022-11-10 Ionis Pharmaceuticals Inc Compuestos y métodos para modular smn2
EP4301766A1 (en) * 2021-03-02 2024-01-10 Ionis Pharmaceuticals, Inc. Purification methods for oligomeric compounds
WO2024053578A1 (ja) * 2022-09-07 2024-03-14 株式会社日本触媒 核酸の製造方法、不純物の除去方法及び不純物が少ない核酸

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JPS60104097A (ja) * 1983-11-08 1985-06-08 Sagami Chem Res Center Ｄνａの精製方法
DE3433649A1 (de) * 1984-09-13 1986-03-20 Gesellschaft für Biotechnologische Forschung mbH (GBF), 3300 Braunschweig Verfahren zur aufreinigung synthetischer oligonucleotide
US6087491A (en) * 1993-01-08 2000-07-11 Hybridon, Inc. Extremely high purity oligonucleotides and methods of synthesizing them using dimer blocks
CN1155887A (zh) * 1994-07-05 1997-07-30 海布里顿公司 用deae-5pw阴离子-离子交换色谱和疏水作用色谱纯化硫代磷酸寡脱氧核苷酸
EP0765334A1 (en) * 1994-07-05 1997-04-02 HYBRIDON, Inc. Purification of oligodeoxynucleotide phosphorothioates using deae 5pw anion ion-exchange chromatography and hydrophobic interaction chromatography
EP0979233A4 (en) * 1997-04-21 2001-03-21 Proligo L L C METHOD FOR SYNTHESIS OF OLIGONUCLEOTIDES IN SOLUTION
JP2000035423A (ja) 1998-05-11 2000-02-02 Tosoh Corp 液体クロマトグラフィ―による核酸の分離法
IL146695A0 (en) * 1999-05-28 2002-07-25 Bio Science Contract Productio Methods of dna purification and purified dna
PT102491B (pt) * 2000-07-10 2003-02-28 Inst Superior Tecnico Processo para producao e purificacao de dna plasmidico
US7345163B2 (en) 2002-08-28 2008-03-18 Quiatech Ab Process for separating and deprotecting oligonucleotides
EP1562971B1 (en) 2002-11-05 2014-02-12 Isis Pharmaceuticals, Inc. Polycyclic sugar surrogate-containing oligomeric compounds and compositions for use in gene modulation
US20050019951A1 (en) * 2003-07-14 2005-01-27 Gjerde Douglas T. Method and device for extracting an analyte
JP4731324B2 (ja) 2003-08-28 2011-07-20 武 今西 Ｎ−ｏ結合性架橋構造型新規人工核酸
DE602005026113D1 (de) * 2004-04-19 2011-03-10 Aventis Pharma Sa Verfahren zur herstellung von plasmid dns in pharmazeutischer qualität
EP3470072A1 (en) 2005-06-23 2019-04-17 Biogen MA Inc. Compositions and methods for modulation of smn2 splicing
DE602007009487D1 (de) 2006-01-27 2010-11-11 Isis Pharmaceutical Inc 6-modifizierte bicyclische nukleinsäureanaloga
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LT3757214T (lt) 2014-04-01 2022-07-25 Biogen Ma Inc. Kompozicijos, skirtos sod-1 ekspresijos moduliavimui

Also Published As

Publication number Publication date
US20190248823A1 (en) 2019-08-15
EP3468981A1 (en) 2019-04-17
IL263639B (en) 2021-09-30
CN109641928A (zh) 2019-04-16
KR20190018467A (ko) 2019-02-22
KR102461234B1 (ko) 2022-10-28
US20210269467A1 (en) 2021-09-02
CA3027288A1 (en) 2017-12-21
WO2017218454A1 (en) 2017-12-21
MX2018015654A (es) 2019-06-10
BR112018075667B1 (pt) 2024-02-20
EP3468981B1 (en) 2024-07-24
JP7075359B2 (ja) 2022-05-25
IL263639A (en) 2019-01-31
AU2017284202B2 (en) 2021-11-04
AU2017284202A1 (en) 2019-01-17
US10947262B2 (en) 2021-03-16
JP2019518759A (ja) 2019-07-04
CN109641928B (zh) 2022-08-30

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Legal Events

Date Code Title Description
B350 Update of information on the portal [chapter 15.35 patent gazette]
B06W Patent application suspended after preliminary examination (for patents with searches from other patent authorities) chapter 6.23 patent gazette]
B15K Others concerning applications: alteration of classification

Free format text: A CLASSIFICACAO ANTERIOR ERA: C07H 1/00

Ipc: B01D 15/30 (2006.01), B01D 15/32 (2006.01), B01D 1

B09A Decision: intention to grant [chapter 9.1 patent gazette]
B09X Republication of the decision to grant [chapter 9.1.3 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 13/06/2017, OBSERVADAS AS CONDICOES LEGAIS