IL263639B - Hydrophobic interaction chromatography for purification of oligonucleotides - Google Patents
Hydrophobic interaction chromatography for purification of oligonucleotidesInfo
- Publication number
- IL263639B IL263639B IL263639A IL26363918A IL263639B IL 263639 B IL263639 B IL 263639B IL 263639 A IL263639 A IL 263639A IL 26363918 A IL26363918 A IL 26363918A IL 263639 B IL263639 B IL 263639B
- Authority
- IL
- Israel
- Prior art keywords
- oligonucleotides
- purification
- hydrophobic interaction
- interaction chromatography
- chromatography
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H1/00—Processes for the preparation of sugar derivatives
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H1/00—Processes for the preparation of sugar derivatives
- C07H1/06—Separation; Purification
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/30—Partition chromatography
- B01D15/305—Hydrophilic interaction chromatography [HILIC]
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/32—Bonded phase chromatography
- B01D15/325—Reversed phase
- B01D15/327—Reversed phase with hydrophobic interaction
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/42—Selective adsorption, e.g. chromatography characterised by the development mode, e.g. by displacement or by elution
- B01D15/424—Elution mode
- B01D15/426—Specific type of solvent
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
- C07H21/02—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with ribosyl as saccharide radical
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
- C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Biochemistry (AREA)
- Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Analytical Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Saccharide Compounds (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201662349970P | 2016-06-14 | 2016-06-14 | |
| US201762492402P | 2017-05-01 | 2017-05-01 | |
| PCT/US2017/037126 WO2017218454A1 (en) | 2016-06-14 | 2017-06-13 | Hydrophobic interaction chromatography for purification of oligonucleotides |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| IL263639A IL263639A (en) | 2019-01-31 |
| IL263639B true IL263639B (en) | 2021-09-30 |
Family
ID=59216016
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| IL263639A IL263639B (en) | 2016-06-14 | 2018-12-11 | Hydrophobic interaction chromatography for purification of oligonucleotides |
Country Status (12)
| Country | Link |
|---|---|
| US (3) | US10947262B2 (enExample) |
| EP (1) | EP3468981B1 (enExample) |
| JP (1) | JP7075359B2 (enExample) |
| KR (1) | KR102461234B1 (enExample) |
| CN (1) | CN109641928B (enExample) |
| AU (1) | AU2017284202B2 (enExample) |
| BR (1) | BR112018075667B1 (enExample) |
| ES (1) | ES2988362T3 (enExample) |
| IL (1) | IL263639B (enExample) |
| MX (1) | MX2018015654A (enExample) |
| NZ (1) | NZ749360A (enExample) |
| WO (1) | WO2017218454A1 (enExample) |
Families Citing this family (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10436802B2 (en) | 2014-09-12 | 2019-10-08 | Biogen Ma Inc. | Methods for treating spinal muscular atrophy |
| AR102198A1 (es) | 2014-10-09 | 2017-02-08 | Regeneron Pharma | Proceso para reducir partículas subvisibles en una formulación farmacéutica |
| US11020686B2 (en) | 2016-08-16 | 2021-06-01 | Regeneron Pharmaceuticals, Inc. | Methods for quantitating individual antibodies from a mixture |
| JP7102401B2 (ja) | 2016-10-25 | 2022-07-19 | リジェネロン・ファーマシューティカルズ・インコーポレイテッド | クロマトグラフィーデータ解析のための方法およびシステム |
| CA3073935A1 (en) | 2017-09-19 | 2019-03-28 | Regeneron Pharmaceuticals, Inc. | Methods of reducing particle formation and compositions formed thereby |
| TWI853823B (zh) | 2018-07-02 | 2024-09-01 | 美商里珍納龍藥品有限公司 | 自混合物製備多肽之系統及方法 |
| WO2020072062A1 (en) * | 2018-10-04 | 2020-04-09 | Gsw Creative Corporation | Portable charging case |
| EA202192422A1 (ru) | 2019-03-05 | 2021-12-09 | Ридженерон Фармасьютикалз, Инк. | Человеческий сывороточный альбумин в составах |
| CN110066789B (zh) * | 2019-05-17 | 2021-04-20 | 通用生物系统（安徽）有限公司 | 一种长链dna引物的hplc纯化改进的方法 |
| CN119874910A (zh) * | 2019-06-07 | 2025-04-25 | 达因疗法公司 | 制备蛋白质-寡核苷酸复合物的方法 |
| CN120505310A (zh) | 2020-02-28 | 2025-08-19 | Ionis制药公司 | 用于调节smn2的化合物和方法 |
| EP4301766A4 (en) * | 2021-03-02 | 2024-12-25 | Ionis Pharmaceuticals, Inc. | PURIFICATION PROCESSES FOR OLIGOMERIC COMPOUNDS |
| CN119855824A (zh) | 2022-09-07 | 2025-04-18 | 株式会社日本触媒 | 核酸的制造方法、杂质的去除方法以及杂质少的核酸 |
| CN117050120A (zh) * | 2022-12-09 | 2023-11-14 | 苏州赛分科技股份有限公司 | 一种纯化小干扰rna的柱层析法 |
| WO2024175051A1 (zh) * | 2023-02-23 | 2024-08-29 | 奥安医药（苏州）有限公司 | 一种疏水层析制备寡核苷酸的方法 |
| WO2025149349A1 (en) | 2024-01-09 | 2025-07-17 | Cytiva Bioprocess R&D Ab | Method for separating nucleic acid molecules |
| WO2025234897A1 (ru) * | 2024-05-07 | 2025-11-13 | Акционерное общество "ГЕНЕРИУМ" | Способ получения тиофосфатных олигонуклеотидов для терапевтического применения |
Family Cites Families (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS60104097A (ja) * | 1983-11-08 | 1985-06-08 | Sagami Chem Res Center | Ｄνａの精製方法 |
| DE3433649A1 (de) * | 1984-09-13 | 1986-03-20 | Gesellschaft für Biotechnologische Forschung mbH (GBF), 3300 Braunschweig | Verfahren zur aufreinigung synthetischer oligonucleotide |
| US6087491A (en) * | 1993-01-08 | 2000-07-11 | Hybridon, Inc. | Extremely high purity oligonucleotides and methods of synthesizing them using dimer blocks |
| CN1155887A (zh) * | 1994-07-05 | 1997-07-30 | 海布里顿公司 | 用deae-5pw阴离子-离子交换色谱和疏水作用色谱纯化硫代磷酸寡脱氧核苷酸 |
| CA2194350A1 (en) | 1994-07-05 | 1996-01-18 | Hybridon, Inc. | Purification of oligodeoxynucleotide phosphorothioates using deae 5pw anion ion-exchange chromatography and hydrophobic interaction chromatography |
| JP2001520660A (ja) * | 1997-04-21 | 2001-10-30 | プロリゴ・エルエルシー | オリゴヌクレオチドの溶液相合成方法 |
| JP2000035423A (ja) | 1998-05-11 | 2000-02-02 | Tosoh Corp | 液体クロマトグラフィ―による核酸の分離法 |
| RU2001135838A (ru) * | 1999-05-28 | 2004-01-20 | Камбрекс Байо Сайенс Инк. (Us) | Способы очистки ДНК и очищенная ДНК |
| PT102491B (pt) * | 2000-07-10 | 2003-02-28 | Inst Superior Tecnico | Processo para producao e purificacao de dna plasmidico |
| US7345163B2 (en) | 2002-08-28 | 2008-03-18 | Quiatech Ab | Process for separating and deprotecting oligonucleotides |
| WO2004041889A2 (en) | 2002-11-05 | 2004-05-21 | Isis Pharmaceuticals, Inc. | Polycyclic sugar surrogate-containing oligomeric compounds and compositions for use in gene modulation |
| US20050019951A1 (en) * | 2003-07-14 | 2005-01-27 | Gjerde Douglas T. | Method and device for extracting an analyte |
| EP1661905B9 (en) | 2003-08-28 | 2012-12-19 | IMANISHI, Takeshi | Novel artificial nucleic acids of n-o bond crosslinkage type |
| NZ549835A (en) * | 2004-04-19 | 2008-12-24 | Centelion | Method for purifying plasmid DNA |
| EP3470072A1 (en) | 2005-06-23 | 2019-04-17 | Biogen MA Inc. | Compositions and methods for modulation of smn2 splicing |
| WO2007090071A2 (en) | 2006-01-27 | 2007-08-09 | Isis Pharmaceuticals, Inc. | 6-modified bicyclic nucleic acid analogs |
| US8969068B2 (en) * | 2008-04-30 | 2015-03-03 | Gradalis, Inc. | Processes for the preparation of highly pure plasmid compositions by enzymatic digestion of colanic acid |
| ES2699827T3 (es) | 2009-06-17 | 2019-02-13 | Biogen Ma Inc | Composiciones y métodos para la modulación de corte y empalme de SMN2 en un sujeto |
| HUE050704T2 (hu) | 2014-04-01 | 2020-12-28 | Biogen Ma Inc | Összetételek a SOD-1 expressziójának modulálására |
-
2017
- 2017-06-13 BR BR112018075667-9A patent/BR112018075667B1/pt active IP Right Grant
- 2017-06-13 NZ NZ749360A patent/NZ749360A/en unknown
- 2017-06-13 EP EP17733243.4A patent/EP3468981B1/en active Active
- 2017-06-13 JP JP2018565677A patent/JP7075359B2/ja active Active
- 2017-06-13 WO PCT/US2017/037126 patent/WO2017218454A1/en not_active Ceased
- 2017-06-13 ES ES17733243T patent/ES2988362T3/es active Active
- 2017-06-13 MX MX2018015654A patent/MX2018015654A/es unknown
- 2017-06-13 AU AU2017284202A patent/AU2017284202B2/en active Active
- 2017-06-13 KR KR1020197000396A patent/KR102461234B1/ko active Active
- 2017-06-13 US US16/309,622 patent/US10947262B2/en active Active
- 2017-06-13 CN CN201780037028.4A patent/CN109641928B/zh active Active
-
2018
- 2018-12-11 IL IL263639A patent/IL263639B/en unknown
-
2021
- 2021-03-12 US US17/199,921 patent/US20210269467A1/en not_active Abandoned
-
2024
- 2024-06-13 US US18/742,011 patent/US20250011357A1/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| KR102461234B1 (ko) | 2022-10-28 |
| NZ749360A (en) | 2025-11-28 |
| ES2988362T3 (es) | 2024-11-20 |
| EP3468981B1 (en) | 2024-07-24 |
| CN109641928B (zh) | 2022-08-30 |
| JP7075359B2 (ja) | 2022-05-25 |
| KR20190018467A (ko) | 2019-02-22 |
| JP2019518759A (ja) | 2019-07-04 |
| MX2018015654A (es) | 2019-06-10 |
| IL263639A (en) | 2019-01-31 |
| US10947262B2 (en) | 2021-03-16 |
| US20190248823A1 (en) | 2019-08-15 |
| BR112018075667A2 (pt) | 2019-04-09 |
| EP3468981A1 (en) | 2019-04-17 |
| BR112018075667B1 (pt) | 2024-02-20 |
| CA3027288A1 (en) | 2017-12-21 |
| CN109641928A (zh) | 2019-04-16 |
| AU2017284202B2 (en) | 2021-11-04 |
| US20250011357A1 (en) | 2025-01-09 |
| US20210269467A1 (en) | 2021-09-02 |
| WO2017218454A1 (en) | 2017-12-21 |
| AU2017284202A1 (en) | 2019-01-17 |
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