KR102461234B1 - 올리고뉴클레오타이드의 정제를 위한 소수성 상호 작용 크로마토그래피 - Google Patents

올리고뉴클레오타이드의 정제를 위한 소수성 상호 작용 크로마토그래피 Download PDF

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KR102461234B1
KR102461234B1 KR1020197000396A KR20197000396A KR102461234B1 KR 102461234 B1 KR102461234 B1 KR 102461234B1 KR 1020197000396 A KR1020197000396 A KR 1020197000396A KR 20197000396 A KR20197000396 A KR 20197000396A KR 102461234 B1 KR102461234 B1 KR 102461234B1
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target oligonucleotide
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KR20190018467A (ko
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로버트 에스. 그롱케
라트네쉬 에스. 조쉬
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바이오젠 엠에이 인코포레이티드
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/30—Partition chromatography
    • B01D15/305—Hydrophilic interaction chromatography [HILIC]
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/32—Bonded phase chromatography
    • B01D15/325—Reversed phase
    • B01D15/327—Reversed phase with hydrophobic interaction
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/42—Selective adsorption, e.g. chromatography characterised by the development mode, e.g. by displacement or by elution
    • B01D15/424—Elution mode
    • B01D15/426—Specific type of solvent
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00—Processes for the preparation of sugar derivatives
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00—Processes for the preparation of sugar derivatives
    • C07H1/06—Separation; Purification
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/02—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with ribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biochemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Saccharide Compounds (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
KR1020197000396A 2016-06-14 2017-06-13 올리고뉴클레오타이드의 정제를 위한 소수성 상호 작용 크로마토그래피 Active KR102461234B1 (ko)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201662349970P 2016-06-14 2016-06-14
US62/349,970 2016-06-14
US201762492402P 2017-05-01 2017-05-01
US62/492,402 2017-05-01
PCT/US2017/037126 WO2017218454A1 (en) 2016-06-14 2017-06-13 Hydrophobic interaction chromatography for purification of oligonucleotides

Publications (2)

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KR20190018467A KR20190018467A (ko) 2019-02-22
KR102461234B1 true KR102461234B1 (ko) 2022-10-28

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US (3) US10947262B2 (enExample)
EP (1) EP3468981B1 (enExample)
JP (1) JP7075359B2 (enExample)
KR (1) KR102461234B1 (enExample)
CN (1) CN109641928B (enExample)
AU (1) AU2017284202B2 (enExample)
BR (1) BR112018075667B1 (enExample)
ES (1) ES2988362T3 (enExample)
IL (1) IL263639B (enExample)
MX (1) MX2018015654A (enExample)
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WO (1) WO2017218454A1 (enExample)

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US10436802B2 (en) 2014-09-12 2019-10-08 Biogen Ma Inc. Methods for treating spinal muscular atrophy
AR102198A1 (es) 2014-10-09 2017-02-08 Regeneron Pharma Proceso para reducir partículas subvisibles en una formulación farmacéutica
US11020686B2 (en) 2016-08-16 2021-06-01 Regeneron Pharmaceuticals, Inc. Methods for quantitating individual antibodies from a mixture
JP7102401B2 (ja) 2016-10-25 2022-07-19 リジェネロン・ファーマシューティカルズ・インコーポレイテッド クロマトグラフィーデータ解析のための方法およびシステム
CA3073935A1 (en) 2017-09-19 2019-03-28 Regeneron Pharmaceuticals, Inc. Methods of reducing particle formation and compositions formed thereby
TWI853823B (zh) 2018-07-02 2024-09-01 美商里珍納龍藥品有限公司 自混合物製備多肽之系統及方法
WO2020072062A1 (en) * 2018-10-04 2020-04-09 Gsw Creative Corporation Portable charging case
EA202192422A1 (ru) 2019-03-05 2021-12-09 Ридженерон Фармасьютикалз, Инк. Человеческий сывороточный альбумин в составах
CN110066789B (zh) * 2019-05-17 2021-04-20 通用生物系统（安徽）有限公司 一种长链dna引物的hplc纯化改进的方法
CN119874910A (zh) * 2019-06-07 2025-04-25 达因疗法公司 制备蛋白质-寡核苷酸复合物的方法
CN120505310A (zh) 2020-02-28 2025-08-19 Ionis制药公司 用于调节smn2的化合物和方法
EP4301766A4 (en) * 2021-03-02 2024-12-25 Ionis Pharmaceuticals, Inc. PURIFICATION PROCESSES FOR OLIGOMERIC COMPOUNDS
CN119855824A (zh) 2022-09-07 2025-04-18 株式会社日本触媒 核酸的制造方法、杂质的去除方法以及杂质少的核酸
CN117050120A (zh) * 2022-12-09 2023-11-14 苏州赛分科技股份有限公司 一种纯化小干扰rna的柱层析法
WO2024175051A1 (zh) * 2023-02-23 2024-08-29 奥安医药（苏州）有限公司 一种疏水层析制备寡核苷酸的方法
WO2025149349A1 (en) 2024-01-09 2025-07-17 Cytiva Bioprocess R&D Ab Method for separating nucleic acid molecules
WO2025234897A1 (ru) * 2024-05-07 2025-11-13 Акционерное общество "ГЕНЕРИУМ" Способ получения тиофосфатных олигонуклеотидов для терапевтического применения

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WO1996001268A1 (en) 1994-07-05 1996-01-18 Hybridon, Inc. Purification of oligodeoxynucleotide phosphorothioates using deae 5pw anion ion-exchange chromatography and hydrophobic interaction chromatography
JP2000035423A (ja) 1998-05-11 2000-02-02 Tosoh Corp 液体クロマトグラフィ―による核酸の分離法

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US6087491A (en) * 1993-01-08 2000-07-11 Hybridon, Inc. Extremely high purity oligonucleotides and methods of synthesizing them using dimer blocks
CN1155887A (zh) * 1994-07-05 1997-07-30 海布里顿公司 用deae-5pw阴离子-离子交换色谱和疏水作用色谱纯化硫代磷酸寡脱氧核苷酸
JP2001520660A (ja) * 1997-04-21 2001-10-30 プロリゴ・エルエルシー オリゴヌクレオチドの溶液相合成方法
RU2001135838A (ru) * 1999-05-28 2004-01-20 Камбрекс Байо Сайенс Инк. (Us) Способы очистки ДНК и очищенная ДНК
PT102491B (pt) * 2000-07-10 2003-02-28 Inst Superior Tecnico Processo para producao e purificacao de dna plasmidico
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US8969068B2 (en) * 2008-04-30 2015-03-03 Gradalis, Inc. Processes for the preparation of highly pure plasmid compositions by enzymatic digestion of colanic acid
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HUE050704T2 (hu) 2014-04-01 2020-12-28 Biogen Ma Inc Összetételek a SOD-1 expressziójának modulálására

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Publication number Priority date Publication date Assignee Title
WO1996001268A1 (en) 1994-07-05 1996-01-18 Hybridon, Inc. Purification of oligodeoxynucleotide phosphorothioates using deae 5pw anion ion-exchange chromatography and hydrophobic interaction chromatography
JP2000035423A (ja) 1998-05-11 2000-02-02 Tosoh Corp 液体クロマトグラフィ―による核酸の分離法

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NZ749360A (en) 2025-11-28
ES2988362T3 (es) 2024-11-20
EP3468981B1 (en) 2024-07-24
CN109641928B (zh) 2022-08-30
JP7075359B2 (ja) 2022-05-25
KR20190018467A (ko) 2019-02-22
IL263639B (en) 2021-09-30
JP2019518759A (ja) 2019-07-04
MX2018015654A (es) 2019-06-10
IL263639A (en) 2019-01-31
US10947262B2 (en) 2021-03-16
US20190248823A1 (en) 2019-08-15
BR112018075667A2 (pt) 2019-04-09
EP3468981A1 (en) 2019-04-17
BR112018075667B1 (pt) 2024-02-20
CA3027288A1 (en) 2017-12-21
CN109641928A (zh) 2019-04-16
AU2017284202B2 (en) 2021-11-04
US20250011357A1 (en) 2025-01-09
US20210269467A1 (en) 2021-09-02
WO2017218454A1 (en) 2017-12-21
AU2017284202A1 (en) 2019-01-17

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